By successful establishment of SCM(Supply Chain Management) System they should make the supply chains speedy and combine production information system with the outcome system and thus they reinforce the competition of the production system of local enterprises in accordance with the rapid decision making This study is to develop the most appropriate production system models through the reflection of JIT system in the SCM which is the necessity of the times and its importance. To achieve this goal the researcher diversifies the prerequisites for the success of the business which will be the strategical factors of the production systems. The objects of this study is to develop the most appropriate production system models and to provide the enterprises with the models which are based on the SCM.

The Robust Design method uses a mathematical tool called orthogonal arrays to study a large number of decision variables with a small number of experiments. It also uses a new measure of quality, called signal-to-noise (S/N) ratio, to predict the quality from the customer's perspective. Thus, the most economical product and process design from both manufacturing and customers' viewpoints can be accomplished at the smallest, affordable development cost. Many companies, big and small, high-tech and low-tech, have found the Robust Design method valuable in making high-quality products available to customers at a low competitive price while still maintaining an acceptable profit margin. A study to analyze and solve problems of a biochemical process experiment has presented in this paper. We have taken Taguchi's parameter design approach, specifically orthogonal array, and determined the optimal levels of the selected variables through analysis of the experimental results using S/N ratio.

To establish the optimal culture systems for production of transferable embryos in Korean Cattle, pregnancy rates of IVF-derived blastocysts according to different culture media, culture method and culture duration were compared. Development of IVF-derived embryos to blastocysts was most effective in YS medium group co-cultre with cumulus cells. Blastocysts cultured for 6 to 8 d in vitro showed higher hatching rate and good quality. Pregnancy rates after transfer of IVF-derived blastocysts cultured for 7 or 8 d were high. Through our experiments, it is considered that improvement of culture media and culture method is necessary for mass production of blastocysts with excellent of good quality in Korean Cattle.

Cordyceps has been used in traditional Chinese medicine more than 2000 year ago. In this study, the new Cordyceps militaris was founded and isolated from O-dae mountain in Korea, and was identified its genetic characteristics. The newly isolation strain HB8 was most closet to Cordyceps militaris W141449 (99.82%), Cordyceps militaris JLCY-LI819 (99.82%) and Cordyceps militaris 4642 (99.81%), respectively. the genotypic result was show that train HB8 was belonging to the Cordyceps militaris genus, therefore, Cordycep militaris HB8 proposed with accession number MT835161. This study we find the optimal condition for production of cordycepin from Cordyceps militaris HB8 was 8 ㎎ /g (200 g of pupa, 1 g of KH2PO4, 0.5 g of K2HPO4, 20 g of glucose, 1 g of MgSO4, 0.05 g of vitamin B1, and 1 ㎎ of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h) and the optimum condition for the production of adenosine was 2.6 ㎎/g (15 g of skim milk powder, 1 g of KH2PO4, 0.5 g of K2HPO4, 20 g of glucose, 1 g of MgSO4, 0.05 g of vitamin B1, and 1 ㎎ of NAA per liter; light condition 300-700 Lux and day/night was 14 h/10 h).

Background : Rehmannia glutinosa L. that is a perennial plant belonging to the family Scrophulariaceae is one of the most popular local income crops. Recently, the cultivation area of Chungnam (2017) accounts for 50.5% (106 ha) of the whole country. In July and August, which is the off-season at the highest price of R, glutinosa L. This experiment was carried out to develop early harvest cultivation technology for the improve farm income. Methods and Results : In order to investigate the growth characteristic and yield of R, glutinosa L. by using the rhizome of 'Tokang' were transplanted four times at intervals of 10 days from mid-March to mid-April 2017 and 2018. Growth characteristics of the above and under ground part were investigated by 3 repetitions for 10 ea harvested in mid-July. Regression analysis was performed on the relationship between the planting time and fresh root weight. The highest emergence rate was 88.7% in the mid-March, and the highest above ground part growth was observed in the plant height, leaf number, leaf length, leaf width, and fresh leaves weight. The number of days to flowering tended to be shortened to 60, 53, 52, and 49 days from the mid-March to the mid-April. The growth of underground part was also highest in mid-March and the total root weight per 10 a was also 1,127.4 ㎏ in mid-March. The faster the planting time, the higher the yield. The results of the linear regression on the roots weight according to the planting time showed that there was a negative correlation relation between the planting time, the marketable roots weight, rhizome weight and total roots weight. Conclusion : The total roots weight improved by 80% in the mid-March than mid-April. Therefore, mid-March is considered optimal planting time for off-season production of R. glutinosa L.

파종기에 따른 울금의 초장, 경직경 및 엽수는 5월 10일 파종구에서 가장 양호하였다. 생육단계에 따른 울금의 생육변화를 살펴본 결과 울금의 생육최성기인 10월에 초장은 121 cm로 가장 길게 나타났고, 경직경 또한 3.45 cm로 가장 두꺼웠다. 엽수는 9월 20일 6매로 조사되었으나 이후 엽수가 감소함이 관찰되었다. 생육단계에 따른 울금 지하경 무게 변화는 생육기간에 경과함에 따라 지하경의 무게 증가가 비례하였으며, 11월 20일 조사에서 울금의 수량이 가장 크게 나타났다. 울금을 건조하는데 가장 적합한 온도는 40℃로 확인되었다. 부형제를 달리하여 제조한 울금환의 관능검사 결과는 향, 색, 맛, 조직감 및 종합적 기호도에서 찹쌀과 꿀을 부형제로 사용한 시험구의 기호도가 가장 우수하였으며 밀가루만을 사용한 시험구의 선호도는 낮게 나타났다.

Monascus purpureus MMK2를 이용하여 황색색소 생산을 위한 배양 조건의 최적화에 관한 결과는 다음과 같다. 실험 균주가 생산하는 황색색소의 최적 배양 조건은 탄소원으로 wheat flour 3.0% 첨가, 질소원으로 0.15%, 인산염으로 의 농도가 0.25% 및 의 농도가 0.15%일 때 가장 높은 황색색소 생성을 나타내었다. 배양 온도는 , 초기 PH가 6.5 및 배양 시간 7일 일 때 황색색소의 생성능이 28.91 unit

Scopolamine and hyoscyamine are important anticholinergic compounds obtained from Hyoscyamus niger. Adventitious roots induced from rhizome of H. niger and roots were cultured in SH medium supplemented with 3% (w/v) sucrose and 0.5 mg/L IBA. Roots were grown rapidly after 10 days of cultures. Scopolamine production was increased 7 times and hyoscyamine production was increased 12 times after 10 day of cultures. SH medium was best in root growth. But, highest scopolamine productivity was observed in WPM medium, followed White medium and best hyoscyamine productivity was resulted in MS medium. Sucrose was increased scopolamine and hyoscyamine production were increased the medium supplemented by sucrose comparing to than those by other carbon sources.

Optimal culture conditions for efficient in vitro propagation and polysaccharide production of Orostachys japonicus were established. The highest growth yield was achieved in 1/2 MS medium, while the lowest growth yield was obtained in 4 MS medium. The patterns of polysaccharide formation were a little similar in all cases, but on MB5 medium, the po]ysaccharide contents of plant were higher than others. Among the modified nitrate levels, effective growth level were obtained in 1/4 N and 1/2 N. High contents of polysaccharide were obtained in 4 N. Different concentration of potassium and calcium did not improve the growth and polysaccharide production. The micropropagated shoots were successfully acclimatized artificial soils.

Monascus rubber KCTC 6122를 이용하여 액체 배양을 통한 실험균의 적색 색소의 생산을 위한 배양 조건의 최적화에 관한 결과는 다음과 같다. 실험 균주가 생산하는 적색색소의 최적 배양 조건은 탄소원으로 rice powder 4% 첨가, 질소원으로 NaN0 0.2%, 인산염으로 HPO의 농도가 0.3%, MgSO의 농도가 0.15%일 때 가장 높은 적색 색소 생성을 나타내었다. 배양 온도는 3, 초기 pH가 6.5, 진탕속도를 15

미치광이풀의 모상근 뱌양에 의해 의학적으로 중요한 물질인 scopolamine과 hyoscyamine 생산을 증진시키기 위해 3종의 곰팡이와 1종의 효모 유래의 생물학적 elicitor를 supernatant와 homogenate의 형태로 처리하였다. Elicitor 중 Candida albicans는 supernatant와 homogenate 모두 scopolamine 생산성을 증가시켰다. Fusarium solani의 homogenate와 C. albicans의 supernatant 처리로 hyoscyamine 함량이 증가되었다. 그러나 나머지 fungus strain도 대조구에 비해 tropane alkaloids의 생산을 다소 증가시켰다. 본 연구에서는 C. albicans가 tropane alkaloids 생산에 적합한 elicitor임으로 구명되었다. 본 연구 결과는 미치광이풀 모상근 대량배양에 의한 tropane alklaoids 대량생산에 기여할 것이다.

A biosurfactant-producing microorganism was isolated from activated sludge by enrichment culture when grown on a minimal salt medium containing n-hexadecane as a sole carbon source. This microorganism was identified as Pseudomonas sp. and it was named Pseudomonas sp. EL-G527. It's optimal culture condition is 2% n-hexadecane, 0.2% NH4NO3, 0.3% KH2PO4, 0.3% K2HPO4, 0.02% MgSO4ㆍ7H2O, 0.0025% CaCl2ㆍ6H2O, 0.0015% FeSO4ㆍ7H2O in 1ℓ distilled water and initial pH 7.0. Cultivation was initiated with a 2% inoculum obtained from starter cultures grown in 30 ㎖ of the same medium in 250 ㎖ flask. They were cultivated at 30 ℃ in reciprocal shaking incubator and the highest biosurfactant production was observed after 4 days.