This experiment was carried out to investigate the effect of harvesting time of Italian ryegrass (Lolium multiflorum Lam; IRG) in spring on dry matter (DM) yield. IRG cultivars ‘Kowinearly’ and ‘Greenfarm’ were seeded at 50 kg/ha and grown on rice paddy fields. There was no difference in growth characteristics between both varieties before winter. However, cold tolerance of Kowinearly was higher than that of Greenfarm, as demonstrated by post-wintering growth characteristics. The heading date of both varieties was affected by the spring weather. The IRG was harvested three times at 5-day intervals beginning from 9 May. The DM yield of Greenfarm was 6,306; 7,335; and 8,109 kg/ha, and that of Kowinearly was 7,498; 9,196; and 10,449 kg/ha at the three consecutive harvests. The delay of the harvesting time for 5 and 10 days increased the DM yield of Greenfarm by 16% and 29% and that of Kowinearly by 23% and 39%, respectively, compared to the yield at first harvest (p < 0.05). Therefore, IRG harvest later than early to mid-May is expected to increase productivity. The feed values of Greenfarm were: 12.2% of crude protein (CP), 34.5% of acid detergent fiber (ADF), 57.7% of neutral detergent fiber (NDF), 61.6% of total digestible nutrients (TDN), and 72.3% of in vitro DM digestibility (IVDMD). For Kowinearly, these values were 16.4% of CP, 30.4% of ADF, 52.7% of NDF, 64.9% of TDN and 79.0% of IVDMD.

This study was conducted to evaluate the growth characteristics and dry matter yields of Italian ryegrass (Lolium multiflorum Lam., IRG) cultivars for three years (2011~2013). The cold-resistance of domestic cultivars was stronger than other foreign varieties. Among early-maturing cultivars, dry matter (DM) yield of ‘Kowinearly’ was significantly higher (8,751 kg/ha) than other cultivars (p<0.05). In medium-maturing cultivars, DM yield of ‘Kowinmaster’ and ‘Tam 90’ was 9,394 and 7,461 kg/ha, respectively. Of the two, the former’s DM yield was higher than the latter’s (p<0.05). Among late-maturing cultivars, DM yield of ‘Hwasan 104’ (9,809 kg/ha) was similar to ‘Hwasan 101’ (9,354 kg/ha), but significantly higher than the other late-maturing cultivars (p<0.05). According to the results obtained from present study, early-maturing cultivars with cold-tolerance and high productivity is required for the paddy field cultivation of an Italian ryegrass cultivar after rice harvest in Cheonan region. Medium- and late-maturing cultivars are more advantageous for the dry field cultivation of the maximum quantity that is possible. In conclusion, to select which cultivar/s to grow, the climate of the growing area, weather conditions, and the cropping system must all be considered

The production scale of mushrooms in Korea is approximately 600 billion won, which is 1.6% of Korea’s gross agricultural output. In Korea, ca. 190,000 tons of mushrooms are harvested annually. Although the numbers of mushroom farms and cultivators are constantly decreasing, total mushroom yields are increasing owing to large-scale cultivation facilities and automation. The recent expansion of the well-being trend has caused an increase in mushroom consumption in Korea: the annual per capita mushroom was 3.9 kg (’13), whichis a little higher than that in Europe. Thus, mushroom export, mainly Flammulina velutipes and Pleurotus ostreatus, has increased since the mid-2000s. Recently, however, it is slightly reduced. Nevertheless, Vietnam, Hong Kong, the United States, and the Netherlands continue to export mushrooms, and Korea has increased its export to Australia, Canada, Southeast Asia, etc. Canned Agaricus bisporus, the first export of the Korean mushroom industry, reached it speak sales in 1977-1978. When Korea initiated trade with China in 1980, the international prices of mushrooms fell sharply, leading to shrinkage of the domestic markets. Spurred by the high demand to develop substitute goods for A. bisporus, the oyster mushroom (P. ostreatus) gained attention since it seemed to suit the taste of Korean consumers. Although the log cultivation technique for oyster mushroom was developed in the early 1970s, it required a great deal of labor. Thus, we developed the shelf cultivation technique, which is easier to manage and allows for mass production. In this technique, the growing shelf is made mafrom fermented rice straw, whichis the only P. ostreatus medium in the world and isused only in South Korea. After then, the use of cotton wastes as an additional material of medium, the productivity. Currently, we are developing a standard cultivation technique and environmental control system that can stably produce mushrooms throughout the year. The increase of oyster mushroom production may boostthe domestic market and contribute to industrial development. In addition, oyster mushroom production technology played a role in forming the basis for the development of bottle cultivation, which made mass production . In particular, bottle cultivation using liquid spawn could allow for the export of F. velutipes and Pleurotus eryngii. In addition, the white varieties of F. velutipes were second developed in the world after Japan. We also developed the new A. bisporus cultivar ‘Saeah’, which is easy to grow in Korea. In hopes to advance the mushroom industry, we will continue to develop cultivars with international competitive power and to improve cultivation techniques.

This study was performed to select the high-yield soybean cultivar suitable for the mid part of Korea and to provide as a basic data for improving productivity of the farmhouse. To this, Daewon as a control and four soybean cultivars such as Uram, Taeseon, Saegeum and Seonpung soybean seeds known that high-yield varieties developed after 2010 were selected. Growth characteristics and functional isoflavone contents of five soybeans were field tested at the field in Goesan, Chungbuk during 2014 and 2015. In comparison with Daewon, maturity date was recorded the earliest at Taesun with 15th Oct. Height of the first pod for the mechanical harvesting have a benefit to Uram (16.8 cm) and Taeseon (14.6 cm), however; shattering percentage of Taeseon were showed the high value of 11.2%. Productivity of Uram, Seonpung and Taesun were increased with 20, 9, and 5 %, respectively, compare to that of Daewon. Total isoflavones contents (μg/g) of five soybeans were ordered as Saegeum (1222.2) > Uram (1111.9) > Seonpung (897.2) > Daewon (885.8) > Taesun (829.5). Total isoflavones contents when soil mulching compare to the non-mulching were increased 206.6 μg/g at Daewon and 460.8 μg/g at Uram soybean.

Simple sequence repeats (SSR), also referred to “microsatellites” consist of tandemly repeated short DNA sequence motifs and have been applied in various marker-based studies. SSRs were isolated and characterized from ‘Heuktari’ and ‘Miso’, which are major oyster mushroom cultivars in Korea, by genome sequencing and bioinformatic analysis. The genome sizes of ‘Heuktari’ and ‘Miso’ were estimated to be 40.8 and 40.3 Mb, respectively, which are larger than those of other P. ostreatus species (PC9 and PC10) and smaller than those of P. eryngii (KNR2312P5). In total, 949 and 968 SSRs were found in the ‘Heuktari’ and ‘Miso’ genomes, respectively. Comparative analysis of five mushrooms including P. ostreatus var. florida (PC9 and PC15) and P. eryngii revealed that the number of SSRs in ‘Heuktari’ and ‘Miso’ were the highest among them. All mushrooms studied showed similar SSR distribution patterns. Tri-, hexa-, and octanucleotide motifs accounted for the top three fractions of all SSRs.

This study aimed to evaluate the quality characteristics of wheat-Makgeolli (WM), a traditional Korean cereal alcoholic drink, using three varieties of wheat, namely Jokyoung (JK), Baegjoong (BJ) and Keumkang (KK). Samples of WM brewed from 100%, 85% and 70% milling rates of the three Korean wheat cultivars were analyzed for alcohol, pH, coloring degree, total acids, soluble solid, free sugars, and organic acids. As the milling rates in wheat decreased, total sugar content in WM increased while the pH of all samples decreased. The WM exhibited 0.95~1.27% in acidity, 10.2~12.5 °Brix in total sugar, and 14~16% in alcohol content. The most organic acids in WM was lactic acid, ranging in all the samples from 85.3~650.3 mg%. The results showed that BJ under a 70% milling rate had the highest reducing sugar contents and 15.97% in alcohol content. The carbohydrate content increased with the milling rate of wheat. Resulting in a positive correlation between carbohydrate content of wheat and total acids, reducing sugars (p<0.001), and alcohol content (p<0.05) in WM. Total sugar content is positively correlated with alcohol and reducing sugar content (p<0.001). Considering the yield, the milling rates will be adjusted to raw material prices.

Among several insect pests in the potato fields, Liriomyza huidobrensis, pea leafminer, is emerging as a new threat to potato plants. Adult females damage the host mesophyll cells host due to ovipositor probing. After egg hatching, the eclosing larva also damage the leaf by mining the mesophyll and causing tissue death. Despite the importance of leafminer in potato production, there is no information available regarding its resistance level to potato cultivars in Korea. Using choice test, 21 potato cultivars consisting of 11 early-maturing, 6 mid-maturing and 4 late-maturing cultivars were evaluated the resistance to pea leafminer in the greenhouse and field condition. In this test, significant differences for resistance were observed. High levels of resistance to leafminers were obtained from cvs. Namsuh, Seohong, Jayoung and Superior. These resistant cultivars might be useful in future breeding programmes.

본 시험은 만개기 기온(15~40oC) 및 생장조절제(GA4+7 +BA와 prohexadione-calcium) 살포가 국내 주요 사과 품종(‘후지’, ‘쓰가루’)과 국내에서 육성한 품종들(‘추광’, ‘감홍’, ‘홍안’, ‘홍금’, ‘홍로’, ‘홍소’, ‘화홍’, ‘섬머드림’, ‘선홍’)의 화분 발아율 및 화분관 신장에 미치는 영향을 조사하고자 실시하였다. 15oC부터 25oC까지 온도가 올라갈수록 화분 발아율과 화분관 신장 길이는 증가되었으나, 30oC 이상의 고온은 화분 발아율과 화분관 신장 길이를 억제시켰다. 30oC 이상의 고온에서 화분 발아율과 화분관 신장 길이가 가장 높았던 국내 육성 사과품종은 ‘선홍’이었다. GA4+7+BA 살포는 무처리구에 비해 화분 발아율 및 화분관 신장 길이를 증진시켰으나, 이러한 효과는 40oC에서 발생하지 않았다. prohexadione-calcium 살포는 화분 발아율과 화분관 신장 길이에 영향을 주지 않았다. 결론적으로, 사과나무 만개기 기온이 30~35oC 정도로 높아지면, 인공수분 후 GA4+7+BA를 살포하는 것이 착과 증진에 좋을 것으로 판단되었다.

Differentiation of Pleurotus eryngii is laborious and time-consuming tasks especially in mycelial status. For development of a method for differentiation of P. eryngii cultivars, simple sequence repeats (SSR) from whole genomic DNA sequence analysis was used for genotyping and two multiplex-SSR primer sets were developed. These SSR primer sets were employed to distinguish 12 cultivars and strains. Five polymorphic markers were selected based on the genotypes. PCR with the each primer produced one to four distinct bands ranging in size from 200 to 300 bp. Polymorphism information content (PIC) values of the five markers were in range of 0.6627 to 0.6848 with an average of 0.6775. Unweighted pair-group method with arithmetic mean clustering analysis based on genetic distances using five SSR markers classified 12 cultivars into 2 clusters. Cluster I and II comprised of 4 and 8 cultivars, respectively. Two multiplex sets, Multi-1 (SSR312 and SSR366) and Multi-2 (SSR178 and SSR277) completely discriminated 12 cultivar and strains with 21 allele with a PIC value of 0.9090. These results might be useful to provide an efficient method for the identification of P. eryngii cultivars with separate PCR reactions. (This work was supported by a grant from the Golded Seed Project (213003-04-3-SBY20), MIFAFF, Republic of Korea.]

본 연구에서는 국내산 고려엉겅퀴를 건강기능식품 소재로 활용시 기초자료 제공하고자 주요 산지별 pectolinarin함량, 총 플라보노이드 함량, DPPH라디칼 소거능 및 ORAC 지수 등을 분석하였다. 산지별 고려엉겅퀴의 pectolinarin함량은 3.95 ± 0.05 ~ 7.29 ± 0.07 mg/g로 강원도 D농장(GW-D)에서 가장 높은 함량을 보였다. 총 플라보노이드 함량은 산지별로 40.42 ± 0.91 ~ 76.70 ± 2.24 mg PNE/g 수준으로 전라남도 A농장(JN-A) 추출물에서 가장 높은 함량을 나타내었고 강원도 A농장(GW-A) 추출물에서(40.42 ± 0.91 mg PNE/g)로 가장 낮은 함량을 보였다. DPPH 라디칼 소거능의 경우, 산지별로 31.25% ~ 81.93%의 활성을 보였고, ORAC 지수는 산지별로 179.62~514.49 μM TE/g로 강원도 D농장(GW-D)과 강원도 E농장(GW-E)에서 각각 514.49, 501.73 μM TE/g 으로 가장 높은 ORAC 수치를 나타내었다. 결론적으로 고려엉겅퀴 추출물은 pectolinarin과 유용성분을 함유하고 DPPH 라디칼 소거능 및 ORAC 활성 등의 항산화활성을 갖는 것으로 나타났다.

서로 다른 숙기를 가진 여섯 개의 자두 품종을 이용하여 과실 발육 단계에 따른 주요 과실 특성의 변 화를 조사하였다. 자두 과실의 산도는 유과기에 가장 높은 수치를 보였다가 과실의 발육이 급속하게 이 루어지는 시기를 기점으로 감소되고 이후 수확기까지 점진적으로 감소하였다. 가용성 고형물 함량은 산 도와 거의 반대로 증가하는 경향을 보였고 숙기가 빠른 품종 일수록 보다 빠른 시기에 변화가 나타났 다. 수확기에 측정 된 산도와 가용성 당함량의 축적량은 품종에 따라 차이가 있어 품종 특이적인 변이 가 있음을 확인하였다. 과실의 발육동안 유리당과 유기산의 조성변화와 수확기에 조사된 총 유리당과 유기산 함량의 검정 결과에서도 품종에 따른 변이를 관찰하였다. 이를 통하여 과실의 발육과정 중 일어 나는 품종 특이적 과실특성의 다양한 변화 양상이 결과적으로 수확기 품종 간 과실품질에 있어서 변이 를 일으키는 주요한 요인으로 작용하고 있음을 간접적으로 확인하였다. 과실의 특성과 관련된 형질들의 값을 이용하여 주성분 분석을 실시한 결과, 본 실험에 이용된 여섯 개의 품종을 대조적인 과실 특성을 가진 세 개의 그룹으로 나눌 수가 있었다. 본 연구를 통해서 밝혀진 과실발육 기간 동안과 수확기에 대 조적인 과실특성을 가진 품종들을 이용한 추가적인 생리 및 육종 연구들은 고품질 자두의 육종에 도움 을 줄 수 있을 것이다.

This study was performed to investigate the content of carotenoid (α-carotene, β-carotene, β-cryptoxanthin, capxanthin, lutein, lycopene, zeaxanthin) and physicochemical characteristics (size, color, dietary fiber, total tannin content) of unripen and ripen persimmons (Diospyros kaki) by seven cultivars. The length of the unripen and ripen persimmons ranged from 40.87-64.75 and 48.80-80.29 cm, the width were from 44.03 to 66.73 and 57.65 to 91.53 cm, respectively. Ripen persimmons were showed in the highest Hunter’s L, a and b values. Total tannin content were resulted of unripen- and ripen persimmons ranged from 7.58 to 19.80 and 2.36 to 5.85 mg/g. Total carotenoid content were from 1.09 to 6.45 and 7.46 to 29.46 μg/g, respectively. From the above results, unripen- and ripen persimmon fruit could be useful for the health functional food.

Agro-morphological characterization of Hibiscus germplasm is a fundamental prerequisite to initiate plant breeding programs. The prime aim of this study was to characterize 107 Hibiscus syriacus genotypes using agromorphological characteristics and to collect useful data to facilitate the breeders for H. syriacus breeding programs. In this study, it has been recorded that this species appears to have innumerable variability in the morphological characteristics among Korean and exotic cultivars. Plant height of Korean and exotic H. syriacus cultivars were recorded and data revealed that the maximum plant height (143 cm) was recorded in ‘Woodbridge’ which had tall-erect plant shape. ‘Serenade’ and ‘Saemaeul’ cultivars had vigorous growth and had plant height of 141 and 137 cm, respectively and both had tall-erect plant shape. The diameters of H. syriacus flower ranged from 4.1 cm in ‘Kakchang’ to 9.4 cm in ‘Pyonghwa’. The data analysis exhibited that analysis flowers of ~ 86% of Korean cultivars had single type of flower’s form compared to exotic cultivars (~ 61%). The data related to flower shape showed that among Korean native cultivars the maximum plants (52.1%) had intermediate type of flowers followed by bowl (26.1%) and flat type (21.8%). Similar trend in exotic cultivars were observed for flower shape. In case of petal shape among total cultivars studied, 27.9% native H. syriacus plants had broard type of petals whereas 17.7 exotic cultivars had intermediate type of petals. Present results indicated high variability among H. syriacus cultivars, which presents great significance for Hibiscus breeding programs and for genetic studies in order to develop new varieties of H. syriacus.

본 연구는 국내 보리 재배시 보리그물무늬병 감염정도를 조사하여 지역과 연차간 종자 전염 발생 변 화와 기상 요인의 상관관계를 확인하기 위하여 수행하였다. 병징은 3월 5일부터 잎에 흑갈색의 작은 반 점들로부터 시작되어 보리의 출수기경에는 잎 전체가 고사되는 증상을 보였다. 감염 종자는 약한 붉은 색의 종피 변색을 나타내었다. 2조와 6조 겉보리 19품종을 시험한 결과 지역에 따라 종자 감염률이 차 이를 보였는데 평균 종자 감염률은 김제와 익산에서 각각 0.09-1.36%와 1.44-2.81%로 익산 지역에서 높은 것으로 조사되었다. 또한, 6조 겉보리가 2조에 비해 병 감염에 의한 종자 감염률에 대해 저항성이 큰 것으로 나타났으며 보리그물무늬병에 의한 연차간 종자감염률의 변화를 보였다. 두 맥종에서 2012년 도에는 0.2%와 0.8%를 보였으나, 2013년도에는 1.4%와 2.8%로 높은 발생을 보였다. 이들 연차간 종자 감염률의 차이에 대한 평균기온과 강수량을 비교하였을때, 평균기온은 병 발생이 많았던 2013년도에 2012년에 비해 저온으로 경과하는 양상이었다. 강수량을 비교한 결과 2013년도에 2012년도에 비해 약 120mm 이상의 많은 강수량을 보였다. 이 결과에서 보리 신장기에서 등숙기까지 저온과 높은 강우시 병 발생에 크게 영향을 미치는 것으로 나타났다.

Differentiation of Pleurotus eryngii is laborious and time-consuming tasks especially in mycelial status. For development of a method for differentiation of P. eryngii cultivars, simple sequence repeats (SSR) from whole genomic DNA sequence analysis was used for genotyping and two multiplex-SSR primer sets were developed. These SSR primer sets were employed to distinguish 12 cultivars and strains. Five polymorphic markers were selected based on the genotypes. PCR with the each primer produced one to four distinct bands ranging in size from 200 to 300 bp. Polymorphism information content (PIC) values of the five markers were in range of 0.6627 to 0.6848 with an average of 0.6775. Unweighted pair-group method with arithmetic mean clustering analysis based on genetic distances using five SSR markers classified 12 cultivars into 2 clusters. Cluster I and II comprised of 4 and 8 cultivars, respectively. Two multiplex sets, Multi-1 (SSR312 and SSR366) and Multi-2 (SSR178 and SSR277) completely discriminated 12 cultivar and strains with 21 allele with a PIC value of 0.9090. These results might be useful to provide an efficient method for the identification of P. eryngii cultivars with separate PCR reactions. (This work was supported by a grant from the Gold Seed Project [Supported by a grant from the IPET (213003-04-3-WTI11), MIFAFF, Republic of Korea.]

Since 2000, the damages of hemipteran pests have severely increased. This study was performed to find the proper monitoring methods and investigate seasonal occurrences of the two apple-infesting stink bug species by the year and to evaluate the orchard rate of infestation and rate of damaged apple. Also the preferences of stink bugs to the crabapple cultivars were investigated in orchards. Compared data obtained from monitoring methods using visible counts, aggregation pheromone traps or mercury light traps, the occurrence density of apple-infesting stink bugs (Plautia stali and Halyomorpha halys) could be monitored more effectively in aggregation pheromone trap than visible count or mercury light trap. The occurrence densities of two species during growing season were low in 2013, but increased in 2014. From the investigations at 25 orchards of eight districts was the infestation rate was at 16.7% in 2013 and 62.1% in 2014 . The occurrence density of stink bugs in crabapples was highest in ‘Purple lemoine’ followed by ‘Professor sprenger’ ‘M. baccata’, ‘Robinson crab’, ‘Sandong pingguo’, damaged fruit rate. The number of damaged holes was high in order as ‘Hopa-A’, ‘Sandong pingguo’, ‘Adam’s crab’, ‘Professor sprenger’, ‘Purple lemoine’.

방사선 동위원소 코발트(60Co)를 유기원으로 한 100Gy의 감마선을 2008년 5월과 9월 두 번에 걸쳐 ‘Clara’ 품종의 캘러스묘에 24시간 처리하였다. 1차에 감마선을 처리하여 유기된 돌연변이 중 착색엽이 분홍색인 변이지를선발하였다. 2010년까지 포인세티아의 특성을 3차에 걸쳐 검정 한 후 ‘Clara Pink’로 명명하였다. 2차로 감마선을 처리한 캘러스묘에서는 2011년까지 세대를 진전시키면서 형질의 안정성 검토 후 착색엽이 연황색인 변이주를 선발하여 계통화하였다. 2012년부터 2013년까지 3차에 걸쳐 특성 검정하였으며, ‘Clara White’로 명명하였다.‘Clara Pink’와 ‘Clara White’는 ‘Clara’와 같은 소형으로적심하지 않은 상태에서 분지가 많이 발생하였다. 두 품종 모두 착색엽이 결각이 없는 난형으로 엽맥 사이에약한 주름이 있으며, 단일처리 약 9주 후 완전히 착색되었다. 그러나 ‘Clara’의 착색엽은 적색인 반면 ‘ClaraPink’는 분홍색이며, ‘Clara White’는 연황색이다.

절화용 국산 스프레이 장미의 정식 후 1차 절곡시기와 2차 절곡방법이 절화품질 및 생산성에 미치는 영향을 조사하였다. 적심 후 절곡시기까지의 경과일수가 길어질수록 주당 엽수, 줄기수, 줄기 굵기 및 1차 측지수가 증가되었다. 특히 ‘White Zen’, ‘Glory Purple’ 및‘Live Pink’는 적심 25일 이후에 충분한 엽수가 확보되었다. 품종별 개화는 절곡시기가 늦어질수록 빨라졌고, 절화장, 마디수와 7매엽은 시험품종 모두 절곡시기가 늦어질수록 우수해지는 경향이었다. 1차 절곡 후 발생한 줄기수는 절곡시기가 늦어질수록 많았으나, 상품 줄기수와수출 가능한 줄기수는 적뢰 25-35일 후 절곡처리에서 대체로 높았다. 2차 절곡방법에 따른 절화장, 소화수 및 줄기 굵기 등의 절화품질은 1차 절곡 후 발생한 줄기 중상품성 있는 줄기 1본을 수확하고 나머지를 절곡한 처리(T2)가 모두 절곡한 처리(T3, T4)보다 우수하거나 유사하였고, 상품성 있는 줄기를 모두 수확하고 비상품 줄기만 절곡한 처리(T1)에서 저조하였다. 상품성 줄기와 수출 가능한 줄기수는 T1 처리가 낮았고 나머지 처리는 유사하였으며, 1차 절곡 후 발생한 상품성 있는 줄기 1본을수확하는 T2 처리가 적절한 2차 절곡방법이라고 생각된다.