This study was conducted to obtain the basic data related to seed characteristics and germination conditions of the Coriandrum sativum L. at different storage condition and temperature. The shape of fruit was oval with light brown color. Fruit was mericarp, biloculate, with one ovule in each locus. The length and width of seed were 1.37 ± 0.067㎜ and 0.52 ± 0.039㎜, respectively. Weight of 1,000 seeds was 6.55 ± 0.15 g. Seeds which were stored at room (15℃) and cold temperature (4℃) in vinyl container showed the highest germination rate (93.3%) under the room temperature germination condition. Percentage of germination in cold and freeze temperature was 20% and 0%, respectively. Germination rate of room storage seeds with paper container increased to about 91.3%, however, decreased in cold (4℃) and freeze storage (−20℃) with paper container (76.7% and 78.0%, respectively). Germination rate of seed in vinyl stock container was better than that of paper stock container. Germination rate of seeds stored at room temperature for 4 years (2010 - 2013) ranged from 80.0% to 91.3%. Therefore, coriander seeds are expected to be stored at room temperature for increasing the germination rate and keeping a long term.

간척지에서 재배될 수 있는 염생식물은 국토의 효율적 이용과 식량의 안정적인 확보 측면에서 매우 중요한 유전자원이다. 본 연구에서는 염생식물인 퉁퉁마디의 작물로서의 이용을 위하여 종자의 발아특성과 염농도에 따른 생육특성을 규명하고자 하였다. 퉁퉁마디 종자는 화피로 둘러싸여 있는 난형으로 연한 갈색을 띄었으며, 수분흡수 후 종피가 파열되면서 유근이 출현하여 발아가 시작되었다. 퉁퉁마디 종자의 발아에서 화피의 영향을 알아보기 위하여 최적 발아온도로 알려진 25℃와 30℃에서 raw seed와 화피제거 종자의 발아율을 조사하였다. Raw seed는 25℃에서 5%, 30℃에서 7%의 발아율을 보인 반면, 화피 제거 종자는 25℃에서 53%, 30℃에서 58%의 발아율을 보여 화피 제거에 의하여 종자의 발아율이 급격히 상승하였다. 따라서 종자의 발아율을 높이기 위하여 500㎛ sieve를 이용하여 화피를 제거할 수 있는 기계적 방법을 개발하였으며 이 방법에 의하여 화피가 제거된 종자를 90% 이상 확보할 수 있었다. 화피 제거 종자의 염농도에 따른 종자의 발아율을 조사한 결과, 0mM에서 53%, 50mM에서 49%, 100mM에서 35% 그리고 200mM에서는 26%로 염농도가 낮을수록 발아율이 높은 것으로 나타났다. 한편, 유묘의 염농도에 따른 생장량 조사를 위하여, 2주된 유묘를 상토에 이식하고 0~200mM의 염처리를 하여 5주간 초장과 분지수를 조사한 결과, 퉁퉁마디의 생장량은 100mM에서 가장 많았다. 따라서 퉁퉁마디의 유묘확보율을 높이기 위해서는 종자의 화피를 제거하여 염농도 0mM인 25~30℃에서 발아를 시키고, 유묘생장단계에서는 100mM 전후의 염농도가 최적조건으로 판단되었다.

This study was conducted to improve the postharvest storage techniques of managing and storing seeds, totest qualities and viabilities of the seeds and to examine the germination rate and the protein expression of Achyranthesjaponica Nakai. The seeds collected from different areas of Je-Cheon and Gwang-Ju were stored with different temperaturesand durations. Two plant growth regulators and two seed priming were treated to investigate their effect on the germinationrates and the days required for germination. The weight of one hundred seed collected in Gwang-Ju was heavier than thosein Je-Cheon. Seed length collected in Gwang-Ju was also longer about 5.12㎜ than those in Je-Cheon about 4.90㎜ andseed width was longer in Gwang-Ju than those in Je-Cheon. The rates of seed germination in two different collection areaswere higher about 2.9 to 13.0% in Gwang-Ju compared to those in Je-Cheon. Comparing its rates with the storing tempera-tures and durations, they were not clearly different in between 4℃ and 25℃ and they also were gradually decreased withgetting longer storing durations. The germination rates treated by plant growth regulators were higher with GA3 than thosewith Kinetin. The highest seed germination rate was appeared at 50 ppm of GA3. Comparing its rates with different seedpriming, they were relatively higher with KNO3 than those with PEG6000. In protein expression patterns between before thegerminating and after the germinating of seeds, more and clear bands were appeared in the seed after the germination com-pared to those before the germination of seeds, especially 10~20kDa. These results showing more and clear bands weremore clearly appeared in Gwang-Ju compared to Je-Cheon. Comparing the protein expression with plant growth regulatorsand seed primings, GA3 was better expression than those with Kinetin and KNO3 was better than those with PEG6000. Moreand clear bands were closely related to the germination rates of seeds and more detailed studies would be required.

Characteristic of seed for Sageretia thea fruits collected from the habitat of harvest season in Jeju and theirgermination ratio in different temperatures were considered. The average weight was 0.2g, average diameter was 7.2㎜,average length was 6.5㎜, and size distribution range was 5.1~10.0mm. The number of seeds per fruit was 1.8, and 1000grain weight was 7.77g with diameter of 3.7㎜ and thickness of 1.7㎜ in size. The fruit maturation was investigated to befrom April 27 to June 1, when the best maturation period was about a week, May 11 to May 18. Also, the fruits grown onMay 4 to May 11 were seen to have the bigger in transverse size and weight with a tendency of the earlier maturation datehas the greater the number of seeds. S. thea seeds pretreated for 24 hours at 50℃ were not germinated, while most of thosepretreated for 24 hours at 4℃ were successfully germinated at any degree of temperature (except at a temperature of 10℃).Particularly, the highest germination rate of 55% was made at 15℃, and plumule and radicle were best grown within thetemperature range of 25℃.

겨울철 도시경관의 향상과 생태적 건전성을 위해 조경용 상록활 엽수 종자 및 묘목의 수요가 빠르게 증가할 것이다. 본 연구는 종가 시나무에 대하여 채종시기와 종자크기에 따른 종자발아 및 유묘생 장의 특성을 구명하고자 수행되었다. 경남 진주시에 위치한 남부산 림자원연구소(N35°09’58.62”, E128°06’23.42”, 67m)에 식재 된 종가시나무에서 2011년 11월부터 이듬해 2월까지 30일 간격으 로 종자를 채집하여 종자크기에 따라서 3가지로 구분하고 종자특 성과 발아 및 유묘생장 특성을 분석하였다. 전반적으로 종자의 수 분함량은 채종을 개시한 11월에서부터 이듬해 1월까지 점차 증가 하는 경향을 나타냈고, 채종시기가 늦어질수록 배의 피해가 증가하 며 특히 대립에서 더욱더 많은 피해가 나타냈다. 발아율은 채종경 과일수와 종자 내 배의 피해 유무와 통계적으로 유의성 있는 상관 을 나타냈고, 종자크기와는 뚜렷한 상관을 나타내지 않았다. 12월 까지 채종된 종자는 높은 발아율을 보였지만 이후에는 점차 발아율 이 낮아지는 경향을 보였고, 발아속도, 발아지수, T50값도 11월과 12월이 상대적으로 양호하였다. 유묘생장은 채종경과일수와 상관 을 나타내지 않았고, 종자크기와 유의성 있는 상관을 나타냈다. 종 자크기가 클수록 총 건중량이 높게 나타냈으며, 11월부터 12월까 지 묘목품질지수가 상대적으로 높게 나타냈다. 결과적으로, 건전 한 종자발아 및 유묘생장을 위해서 종가시나무의 채종시기는 12월 까지가 적합하며 상대적으로 큰 종자를 선별하는 것이 적합할 것으 로 판단되었다.

This study was conducted to investigate the quality of seeds, the germination rates and the days required for germination, to examine the patterns of protein expressions during the germination and to improve the techniques of managing and storing seeds and viability of the seeds of Lithospermum erythrorhizon Sieb. et Zucc. After collecting and harvesting seeds, they were classified to white and brown colors of seed coat through testing their seed size, weight, and quality. The germination rates, the days required for germination, and the protein expressions were examined with different colors of seed coats, storing temperatures and durations by treating the different plant growth regulators and primings. One hundred seed weight of white color was heavier about 1.17 g than those of brown one about 0.81 g. The germination rates in white color of seed coat was higher, 3.05 ~ 5.75%, than those in brown one. Its rates were decreased with getting longer in storage durations. There was no big differences on germination rates between storage temperatures. The plant growth regulator of GA3 and Kinetin was affected to improve the seed germination. GA3 increased the seed germination clearly at 25 ppm level, while kinetin increased it gradually from 25 to 100 ppm levels. In germination by seed primings, PEG6000 made higher germination rate with increasing their levels, whereas KNO3 increased the germination until 100 mM level and then decreased it with 200 mM unlike PEG6000. The protein expressed during the seed germination were appeared more and clearer bands in the seed after germination, especially 20 ~ 30 kDa, compared to those in the seed before germination. These results showing more and clearer bands were positively related to the germination rates which were different by seed colors, storage temperatures and durations, and plant growth regulators and primings.

본 연구에서는 검정콩 발아를 위한 수침조건을 설정하고 발아시 발아수의 염 농도를 달리하여 그에 따른 발아율, 피니톨 및 기타 품질특성변화를 살펴보고자 하였다. 그 결과 수분 흡수율의 변화는 수침초기 6시간 까지는 급격한 증가를 보였으며, 수침 시간 및 수침 온도가 증가할수록 수분 흡수율이 증가하는 것으로 나타났다. pH는 수침 온도가 높을수록 낮아지는 것으로 나타났다. 안토시아닌 용출율은 수침 시간이 증가할수록 점차적으로 증가하는 것으로 나타나 수침 시간은 단시간에 필요한 수분 흡수율을 나타나내는 25℃, 6시간이 적당한 것으로 나타났다. 발아시 검정콩 발아율은 염 농도가 증가할수록 감소하는 것으로 나타났으며, 피니톨 함량을 분석한 결과 염 농도 0.7%일 때 피니톨함량이 높게 나타났다. 총 페놀성 화합물 함량은 염 농도 0.3～0.5%에서 높은 것으로 나타났으며, 플라보노이드 함량은 염 농도 0.1%에서 높은 것으로 나타났다. 본 연구를 통하여 검정콩 발아시 염 스트레스 처리는 혈당강하 성분인 피니톨 함량을 증대하여 검정콩의 기능성 식품소재로서의 가치를 높일 수 있을 것으로 사료된다.

This study was conducted to determine the effects of seed storage temperature and pre-treatment on seed ger-mination, seedling quality and vigor index of wild Trichosanthes kirilowii and Trichosanthes kirilowii var. japonica. As a resultthe highest germination rate of T. kirilowii was the 95% at seed stored in room temperature and then soaking for 24 hours indH₂O. And the highest germination rate of T. kirilowii var. japonica was 96% at seed stored in 2℃ and then soaking for 24hours in GA₃(100ppm). But the seed germination rate was non-significance in pre-treatment at seed stored in roomtemperature 2℃ .Seedlings of T. kirilowii and T. kirilowii var. japonica showed not only the best seedling quality but alsoseedling vigor index in seed stored at 2℃ and then soaking for 24 hours in GA₃(100ppm). In this research, T. kirilowii and T.kirilowii var. japonica seed were stored in room temperature or 2℃, and then sown in peat moss, seed germination rate wasmore than 90% and production of superior quality seedlings.

찰벼 4품종을 두 온도조건(저온; 15℃, 대조; 25℃)에서 왕겨의 유무에 따라(정조 및 현미) 발아특성을 조사한 결과, 현미 발아율은 품종간 차이가 없었으나 정조 발아율은 저온 에서 품종간 차이가 컸다. 종자를 침윤시키면 온도나 왕겨 의 유무에 관계없이 수분이 급격히 증가하다가 30%에서 더 이상 증가하지 않고 정체하는데 이때 정체기에 도달하는 시 간은 온도가 높을수록, 정조보다 현미가 빨랐다. 그러나 수 분정체기에 도달하는 시간은 품종간 차이가 있고 저온에서 차이가 컸다. 수분흡수시간은 발아율, 발아세, 발아속도, 평 균발아일수와 모두 높은 부의 상관을 보여 초기 수분흡수시 간이 빠를수록 발아를 촉진하는 것으로 나타났다. 침윤 24 시간 뒤 guaiacol peroxidase활성은 처리 및 품종간에 큰 차 이를 나타냈으며 발아세와 발아속도와 정의 상관이 있었으 나 최종발아율과는 상관이 낮았다. Catalase활성은 발아율, 발아속도 등과 상관이 매우 낮았으며, 용해성 단백질함량 및 maltose농도도 발아특성과 상관이 낮았다. 종자활력검사 (TTC활력검사)와 표준발아율검사(25℃)에서 발아율이 높 더라도 정조는 저온에서 품종간 발아율의 변이가 커 종자활력검사만으로 발아율을 예측하기 힘들었다. 따라서 초기의 종자활력은 TTC검사와 guaiacol peroxidase활성을 함께 고 려하는 것이 더 정확한 것으로 생각된다. 또 품종간 발아율 의 변이를 줄이고 발아속도를 높혀 균일한 발아를 유도하기 위해서는 적정범위에서 가능한 온도를 높혀 수분흡수를 촉 진시켜야 한다.

This study was investigated to evaluate germination rate of Astragalus membranaceus B. in Korea as affectedby storage temperature, germination temperature and storage period of seed. The highest germination rate was obtainedfrom condition of 25℃ in germination temperature. Seeds were stored at −20℃ and 5℃ for 8 weeks has showed higher ger-mination rate than one at room temperatures. The germination rates showed significantly difference by harvested year of2010, 2011 and 2012. The seed of A. membranaceus in harvested year of 2011 and 2012 had germinated well. On the otherhand, seeds in harvested year of 2010 were not nearly germinated. Consequently, the longer storage period after seed har-vest lower germination rate and seed vigor as well.

This study was carried out to compare the characteristics of seed germination and the first stage of growth in Cynanchum wilfordii (Maxim.) by different light conditions such ass fluorescent light, dark and light emitting diode (LED). There was not much difference from the germination rate of C. wilfordii (Maxim.) in different light conditions for 10 days. The highest of germination rate occurred over 91.11 percentage by LED red condition at 26℃. Also mean germination velocity and promptness index were represented the highest results of 2.3 ea/day and 52.67 under red light, respectively. But mean germination time under both LED red and blue light was represented low data that it takes over three days for germinating of seed. Even though handling both LED red and blue lights, the germination uniformity was not constant. These results may help our understanding of variations in germination characteristics for C. wilfordii (Maxim.) seeds treated under different light conditions.