이 시험은 도입남양재 원목에 부착 또는 서식하는 유해병해충의 방제를 목적으로 실시하는 해수수침소독에 있어 농약의 소요량기준을 설정함을 목표로 실시한 것으로써 결과를 요약하면 다음과 같다. 1. 우리나라에 도입되는 남양재 원목의 평균 길이는 11.12m이었으며 직경은 69.55cm이었다. 2. 원목의 전재적에 대한 부상재적은 평균 이었다. 3. 원목의 전재적에 대한 부상표면적의 비의 값은 2.3356( 신뢰수준)이었다. 4. 원목의 전재종 대 부상표면적에 대한 표본추출오차는 1본선내 원목간의 오차(0.2087)가 10본선간의 오차(0.2952)보다 적었다. 5. 원목 당 농약의 적정소요량은 수동식살포기를 이용하는 경우 0.368cc, 그리고 동력분무기를 이용하는 경우는 0.295cc이었다. 6. 예를 들어 남양재 원목 을 도입하여 동력분무기로 해수수침소독을 실시하는 경우 농약소요량은 다음과 같이 계산된다. .

종자 산균제로써 Dithane M-45는 종래 사용해오던 유기수은제 보다 약간 우세한 살균력을 나타냈으며 적정농도는 1000ppm이였고, 적정시간은 4-6시간이었다. 그러나 Fusarium spp.에 대해서는 어느 농도에서도 흡족할만한 살균력을 나타내지 못하였다.

콩 탄저병에 대한 우수한 종자소독제를 선발하고자 병원균을 종자와 토양접종한 다음 각종소독제에 의한 시험과 조지대에 의한 약효비교시험을 수행하였다. 종자소독제는 Arasan, Orthocide, Phygon-XL, Luberon, Mercuron 및 P.T.A.B등을 공시하였다. Arasan은 모든 시험구에서 가장 효과가 좋았으며 종자접종구에서는 모든 공시 약제의 효과가 현저하였으나 약제간에 유의성이 없었고 토양접종구에석는 루베론을 제외한 약제는 무처리보다 효과가 있었으나 약제간에 유의성은 없었다 조지용법에 의한 시험에서는 약효가 일치되지 않았으나 경향은 비슷하였다.

본 연구는 대전, 충청지역 초음파실 종사자를 대상으로 병원감염관리에 관한 설문 조사를 실시하여 분석 하였고, ATP를 이용하여 임상에서 사용되는 초음파 Probe의 오염도를 측정하였으며, 70% 알코올 이용하여 소독한 후 결과를 확인하였다. 기본적인 조사 대상자의 일반적인 특성 및 직종 별 대상자의 특성으로 나누어 학력, 직종, 초음파 전문자격증 보유 유무, 기관 내 감염 담당 부서 및 감염 교육의 여부를 분석하였다. 검사 후 젤 제거 여부 및 방법, Probe의 소독여부 및 변수 별 상관분석을 수행하여 초음파 Probe의 세균감염에 관한 인식 및 세척 및 소독에 대한 수행 정도를 분석하였다. 검사 후 Probe 세척에 관하여 수건을 가장 많이 사용하였으며, Gel 용기는 3개월 이상 교환하지 않는 것으로 나타났다. 70% 알코올 소독 후 ATP 오염도는 1055.4±944.2에서 133.5±93.2으로 감소하였고 통계적으로 유의한 것으로 분석되었다.(ρ<0.01) 병원 균종은 CNS, Gram positive bacillus, Micrococcus species 검출되었다. 이를 해결하기 위해 시중에서 손쉽게 구할 수 있는 70% 알코올을 이용하여 소독을 실시하였으며 소독 후 병원균종은 검출되지 않았다. 연구 결과 초음파실을 관리하는 의료인 또는 의료기사 등은 감염관리에 대한 주기적인 교육과 감염예방에 대한 노력이 필요하다고 볼 수 있으며, 70% 알코올은 병원균 사멸에 효과적인 것으로 나타났다. 따라서 의료기관은 적극적인 병원감염관리 교육을 실시하여 종사자들의 병원감염에 관한 인식을 개선하고, 환자 감염 예방에 기여해야 할 것이다. 또한 본 연구 결과를 적절히 활용한다면 초음파 Probe를 매개로 한 감염 예방에 도움이 될 것으로 사료된다.

본 연구는 황토유황을 활용하여 벼 키다리병 발병을 최소화할 수 있는 최적 종자소독조 건을 선발하기 위해 수행되었다. 키다리병에 이병된 ‘신동진’ 품종 벼 종자를 공시하였고 황토유황 농도, 침지온도, 시간, 온탕여부를 구분하여 실내 및 온실검정을 통해 침지시간은 48시간, 침지농도는 1%로 최적 소독조건을 선발하였다. 그리고 모판과 포장시험을 수행하 여 온탕처리 후 20℃, 1% 황토유황에 48시간 침지 처리구와 30℃, 1% 황토유황에 48시간 단독 침지할 때 가장 효과가 높았다는 것을 확인하였고 위 처리조건으로 소독한 종자가 육 묘, 이앙, 출수기를 거치면서도 100%의 높은 방제효과가 유지되는 것을 확인할 수 있었다. 그러나 20℃에서 1% 황토유황으로 침지한 처리구에서는 포장에서 78.2%의 낮은 방제가를 보였다. 또한, 온탕소독만 수행한 처리구에서도 60℃, 10분간 충실히 수행될 경우 효과적인 벼 키다리병 방제가 가능함을 보여주었지만 온탕처리 후 황토유황으로 추가 침지함으로서 많은 양의 종자를 소독할 때 좀 더 나은 키다리병 방제 효과를 가져다 줄 수 있을 것으로 판단된다. 이상의 결과를 종합하여 볼 때 농가에서 온탕소독기와 종자소독기 둘 중 한가지 만 구비하더라도 황토유황을 이용하여 충분한 소독효과를 낼 수 있기 때문에 현장에서 보 다 쉽게 활용될 수 있을 것으로 판단되며 제조방법이 쉽고 비용이 적게 드는 황토유황의 특성상 농가의 경영비 절감효과도 클 것으로 기대된다.

In this study, we examined the suitability of ten disinfection models for predicting the inactivation of Artemia sp. via single or combined physical and chemical treatments. The effect of Hydraulic Retention Time (HRT) on the inactivation of Artemia sp. was examined experimentally. Disinfection models were fitted to the experimental data by using the GInaFiT plug-in for Microsoft Excel. The inactivation model were evaluated on the basis of RMSE (Root Mean Square Error), SSE (mean Sum Square Error) and r2. An inactivation model with the lowest RMSE, SSE and r2 close to 1 was considered the best. The Weibull+Tail model was found to be the most appropriate for predicting the inactivation of Artemia sp. via electrolytic treatment and electrolytic-ultrasonic combined treatment. The Log-linear+Tail model was the most appropriate for modeling inactivation via homogenization and combined electrolytic-homogenization treatment. The double Weibull disinfection model was the most suitable for the predicting inactivation via ultrasonic treatment.

Background : This experiment was conducted to select GAP applying seed disinfectants in Astragalus membranaceus and Platycodon grandiflorum. Methods and Results : We carried out the chemical efficacy and injury test. For the efficacy test, we investigated fungal detection rate by seed disinfectants and for the chemical injury, we investigated germination rate and emergence rate by seed disinfectants in reference amount and fold amount. These experiments carried out two times. The results obtained are as follows. In the experiment for seed disinfectants selection of Astragalus membranaceus, all tested chemicals such as Tebuconazole emulsifiable concentrate(EC), Thiophanate-methyl + Triflumizole wettable powder(WP), Prochloraz copper chloride complex+Tebuconazole suspension concentrate(SC), Prochloraz emulsifiable concentrate(EC), Fludioxonil wetting liquid(WL) and Hexaconazol+Prochloraz emulsifiable concentrate(EC) had control value of 80% or above against seed contaminated fungi. However two chemicals such as Tebuconazole EC and Prochloraz copper chloride complex+Tebuconazole SC and two chemicals such as Prochloraz EC and Hexaconazol+Prochloraz EC exhibited chemical injury significantly in reference amount and in fold amount respectively, compared to non treated control. In the case of seed disinfectants selection of Platycodon grandiflorum, Prochloraz copper chloride complex+Tebuconazole SC, Prochloraz EC and Hexaconazol+Prochloraz EC had control value of above 80% against seed contaminated fungi except Thiophanate-methyl+Triflumizole WP and Fludioxonil WL. However Hexaconazol+Prochloraz EC and Prochloraz copper chloride complex+Tebuconazole SC exhibited chemical injury significantly in reference amount and in fold amount respectively, compared to non treated control. Conclusion : From the above results, we finally selected three items of seed disinfectants including Thiophanate-methyl+Triflumizole WP and Fludioxonil WL in Astragalus membranaceus and Prochloraz emulsion in Platycodon grandiflorum.

Background : The area of ginseng cultivation has nearly exhausted in the main ginseng growing area in Korea but virgin fields need for ginseng cultivation due to continuous cropping injury. To count for the decrease of the virgin fields of ginseng cultivation, technical improvement to increase, or maintain the amount of harvest are necessary in ginseng cultivation. The Ginseng Rain-sheltered Shade House is one of the efficient cultivation techniques as reducing the damages caused by natural disaster such as blight, high temperature & humidity, dry, and heavy rain & snow. However a soil description needs to be developed to reduce the period of re-cultivation because ginseng has to be cultivated at the only one ginseng rain-sheltered house without soil or cultivation change. Methods and Results : This study was carried out in the ginseng rain-sheltered house where ginseng had cultivated and harvested one time. Each section of Cultivation of sudan grass+flooding, rice straw+flooding, and flooding was covered with vinyl and then, all areas were solarized through the summer season. The soil temperature was inspected at 5cm, 10cm, 15cm, and 20cm below ground. As a result, the soil temperature of the place with vinyl covered and solarized was approached to 40℃ regularly during the period of high temperature in July and August. After the solarization, the inspection result of Cylindrocarpon destructans spore density checked by Real time PCR was that C. destructans spores were not found at the place where sudan glass+flooding and rice straw+flooding were used. However, the detected number of C. destructans spores in the inspection condition was roughly 9 at the soil samples from the fields which were flooded and solarized, and 33 at those from the repeatedly cultivated fields. The evaluations of aerial part growth by measurements were good in the following order: Sudan grass+flooding, rice straw+flooding, and flooding. Conclusion : The purpose of this study was to reduce the period of ginseng re-cultivation in a ginseng rain-sheltered shade house. The incidence rate of the pathogens distantly decreased by the treatments of sudan glass + flooding, rice straw+flooding, and solarization.

Background : Root diseases caused by Cylindrocarpon destructans and Fusarium solani decrease the yield and quality of ginseng. Cylindrocarpon root rot is a major disease caused by replanting failure in ginseng garden. Methods and Results : Solarization was done in the infested soil of the greenhouse for summer season (from July 24 to Autumn 31, 2014) after putting green manure (Sudan grass) and calcium cyanamide (CC) into the soil. Mycelium and conidia of C. destructans died at 4 0℃ after 15 hours, and 45℃ after 5 h, but it did not die at 35℃ after 15 h. Those of C. destructans died after keeping it for 2 hours daily at 40℃ for 9 days, and 45℃ for 8 days, but did not die at 38℃ for 9 days. Maximum soil temperature was 55.4℃ in 5 cm depth, 48.7℃ in 10 cm, 44.7℃ in 15 cm, 42.5℃ in 20 cm, and 31.9℃ in 30 cm by putting green manure into the soil and solarization. Reduction of sudan grass increased electrical conductivity (EC), organic matter, P2O5, K, and Mg, while decreased pH, NO3-N, and Na. Addition of calcium cyanamide and urea gave a negative effect on the growth of ginseng because EC and NO3-N were increased excessively than the optimal range. Solarization using green manure mixed with CC was the most effective in decreasing soil-borne pathogens of 2-year-old ginseng. But the root disease that occurred between single treatment of sudan grass and the treatment mixed with calcium cyanamide showed not a significant different. Addition of calcium cyanamide showed the decrease of root weight because leaves were dead early by a excessive increase of EC and NO3-N. Conclusion: Soil disinfection using green manure and solarization in greenhouse was effective in inhibiting root rot, however, it did not completely kill the soil-borne pathogens.

Background: Root diseases caused by Cylindrocarpon destructans and Fusarium solani decrease the yield and quality of ginseng. Cylindrocarpon root rot is a major disease caused by replant failure in ginseng fields. Methods and Results: Solarization of infested greenhouse soil was carried out during the summer season after applying green manure (Sudan grass) and Calcium Cyanamide (CC) on the soil. Mycelium and conidia of C. destructans died at 40℃ after 15 h, but they did not die at 35℃ after 15 h. They also died after keeping the soil at 40℃ for 2 h daily for 9 days, and at 45℃ for 8 days, but they did not die at 38℃ for 9 days. Maximum soil temperature was 55.4℃ at 5 ㎝ depth, 48.7℃ at 10 ㎝, 44.7℃ at 15 ㎝, 42.5℃ at 20 ㎝, and 31.9℃ at 30 ㎝ by incorporating green manure into the soil and using solarization. Solarization using green manure mixed with CC was the most effective in decreasing soil-borne pathogens of 2-year-old ginseng. However, the addition of CC decreased the root weight due to the increase in EC and NO3-N. Conclusions: Soil disinfection using green manure and solarization in a greenhouse environment was effective in inhibiting root rot, however, it did not completely kill the soil-borne pathogens.

his study was carried out to investigate antimicrobial activities of water extracted Pleurotus ostreatus var Heuktari (PO), and their application to F. fujikuroi Nirenberg growth inhibition material. Various organic solvents (chloroform, hexane, ethyl acetate, ethanol) were tested to investigate the antimicrobial activities of Pleurotus ostreatus var Heuktari against F. fujikuroi Nirenberg. Chloroform, hexane, ethyl acetate and hot water extracts had no antimicrobial activities, but butanol extract showed 2.5% strong activities in order of F. fujikuroi Nirenberg disk diffusion test. Then we observe Antifungal activity using green house. Bed soil and mushroom extract mixed at 10%, 7.5%, 5%, 2.5%, 1% total bed soil mass. Screening of Antifungal activity was tested two periode 18day and 25day. The Antifungal activity rate of each period and extract density was 7.5% mixed bedsoil has investigated 80% Antifungal activity rate. The result indicates that the Pleurotus ostreatus var Heuktari extract using F. fujikuroi Nirenberg registance, which shows the development of organic agriculture seed disinfectant.

In this study, we discussed about the application of the single physical and chemical treatment processes and the physical-chemical complex treatment processes on the inactivation of Artemia sp. in order to satisfy the USCG Phase Ⅱ (United States Coast Guard). The results showed that initial disinfection rate of ultrasonic process in single batch process is higher than that of electrolysis. However, the inactivation rate showed slower than electrolysis. The inactivation rate of Artemia sp. on the single continuous treatment process ranked in the following order: homogenizer 〉 electrolysis 〉 ultrasonic process. Inactivation rate of Artemia sp. in continuous homogenizer-electrolysis complex process was reached at 100% immediately. A synergistic effect of ultrasonic–electrolytic complex process was found to be a small. The order of processes in a complex process did not affect the disinfection performance.

Formation of disinfection by-products (DBPs) including trihalomethans (THMs) and haloacetic acids (HAAs) from chlorination of six different species (Chlorella vulgaris, Scenedesmus sp., Anabaena cylindrical, Microcystis aeruginosa, Asterionella formosa and Aulacoseira sp.) of algal extracellular organic matter (EOM). The EOM characteristics evaluation of six algal species reaching at the stationary phase in the growth curve showed most of its SUVA254 showed below 1 and this means hydrophilic organic matter is much higher than hydrophobic organic matter. Chloroform formation potential (CFFP), dichloroacetic acid formation potential (DCAAFP) and trichloroacetic acid formation potential (TCAAFP) were mainly composed of THMFP and HAAFP in the EOM of various algal species. In the case of THMFP/DOC and HAAFP/DOC values, EOM of blue-green algae has appeared highest and EOM of green algae and diatom in order. THMFP/DOC was higher than HAAFP/DOC in EOM of blue-green algae. In comparison of formation potential by unit DOC composed of HAAFP in algal species EOM, DCAAFP/DOC was 1.5 times to 7.5 time higher than TCAAFP/DOC in the EOM of blue-green algae, while DCAAFP/DOC was found to be relatively high compared to TCAAFP/DOC in the EOM of green algae and diatom.

Food waste, food leachate and livestock wastes from an usual farm and a farm using much disinfectant were mixed to incubate within anaerobic serum bottle for BMP test. The methane yield rate and lag phase were determined by the modified Gompertz model and the Logistic model. The maximum methane yield rates by the modified Gompertz model were 15.9 ~ 41.0 mL CH4/g VS and higher than by the Logistic model. The modified Gompertz model was more appropriate than the Logistic model to have higher determination coefficient R2. The methane fermentation of mix with sole livestock waste from the farm using much disinfectant had ninefold lag phase and 40% or lower maximum yield rate comparing with the mix with sole usual livestock waste. The methane yield rate from a tonne of mix was increased as the ratio of food waste and food leachate increased. The cumulative methane yield was in the proportion of 40 m3 to a tonne of food wastes. The results of BMP test were analyzed by a response surface methodology (RSM) and modelized to a binomial expression, which was verified by analysis of variance (ANOVA) and to be appropriate for this case.