The objective of this study was to investigate the effect of amino acid and vitamin C supplementation on the quality attributes of top round (TR) muscle from Korean cattle (Hanwoo). Sixteen-month-old steers were divided into three different feeding groups and raised until they were 28 months old. A control group (9 heads) was fed a basal diet and rice straw, whereas two experimental groups (8 heads/group) were fed a basal diet and rice straw supplemented with rumen-protected amino acids (≥16.0% L-lysine monohydrochloride, ≥8.0% DL-methionine) and vitamin C at a dose of 100 g/head/day (AA+VC group) or only vitamin C at 100 g/head/day (VC group). After slaughter, TR muscle (M. semimembranosus) was collected from carcasses and used to measure the quality attributes. Crude fat content was significantly higher (p<0.05) in the AA+VC group than in the VC group. No significant difference was observed for water-holding capacity and shear force value between the control, AA+VC, and VC groups. Contents of the free amino acids (FAAs) glycine, aspartate, and glutamate were significantly higher (p<0.05) in the VC group than in the control group. Sensory evaluation of tenderness and flavor preference revealed significantly higher scores (p<0.05) in the AA+VC group than in the control group. These findings suggest that vitamin C supplementation increases the contents of some FAAs, and the combined supplementation of amino acids and vitamin C improves the marbling and sensory attributes of TR muscle in Hanwoo.

A total of 30 Korean native pigs (gilt 15, boar 15) were used to investigate the carcass characteristics, meat quality, amino acid, and fatty acid composition by gender. The carcass weight of boars were significantly higher than gilts, whereas the carcass yield of gilts had significantly higher than boars (p<0.01). Boars had significantly higher moisture contents in loin muscle than gilts, whereas the protein contents of loin muscle had significantly higher in gilts than boars (p<0.01). In the results of meat quality analysis, the cooking loss (p<0.01), shearing force (p<0.05), lightness (L) and yellowness (b) in meat color (p<0.05) were significantly higher, but the pH was significantly lower (p<0.01) in gilts compared with boars. Arginine (p<0.05), alanine, aspartic acid, histidine, leucine, lysine, phenylalanine, serine, threonine and tyrosin (p<0.01) for gilts were significantly higher than those for boars. The results of fatty acid composition showed that gilts had significantly higher contents of C16:1n7, C18:1n9, C20:1n9 (p<0.01) than boars in intramuscular fat, whereas boars had significantly higher contents of C18:2n6, C20:4n6 (p<0.01) and C18:3n3 (p<0.05) than gilts in intramuscular fat.

Sulphur deficiency has become widespread over the past several decades in most of the agricultural area. Oilseed rape (Brassica napus L.) is a very sensitive to S limitation which is becoming reduction of quality and productivity of forage. Few studies have assessed the sulphur mobilization in the source-sink relationship, very little is known about the regulatory mechanism in interaction between sulphur and nitrogen during the short-term sulphur deficiency. In this study, therefore, amount of sulphur and nitrogen incorporated into amino acids and proteins as affected by different S-supplied level (Control: 1 mM SO4 2 , S-deficiency: 0.1 mM SO4 2 , and S-deprivation: 0 mM SO4 2 ) were examined. The amount of sulphur in sulphate (S-sulphate) was significantly decreased by 25.8% in S-deprivation condition, compare to control, but not nitrogen in nitrate (N-nitrate). The markedly increase of sulphur and nitrogen incorporated amino acids (S-amino acids and N-amino acids) was observed in both S-deficiency and S-deprivation treatments. The amount of nitrogen incorporated proteins (N-protein) was strongly decreased as sulphur availability while the amount of sulphur incorporated into proteins (S-protein) was not affected. A highly significant (p<0.001) relationship between S-sulphate and S-amino acid was observed whereas the increase of N-amino acids is closely associated with decrease of N-proteins. These data indicate that increase of sulphur and nitrogen incorporated into amino acids was from different nitrogen and sulphur metabolites, respectively.

The objective of this study was to investigate the effects of NEAA and leptin supplemented to in vitro culture medium on the developmental competence of porcine embryos after intracytoplasmic sperm injection (ICSI), and to modify the culture condition to improve the quality and the development of ICSI-derived porcine embryos in vitro. After ICSI, the putative zygotes were then cultured in PZM-3 medium with/without NEAA or leptin. The proportion of embryos that developed to the blastocyst stage significantly increased when 1% NEAA (24.62%) was added to the medium compared with 2% NEAA and no NEAA group (17.24% and 20.24%, respectively, p<0.05). The effect of different concentration of leptin (0, 10, 100, 500 ng/ml) was evaluated on the development of porcine ICSI embryos cultured in vitro. In case of blastocyst formation, 100 ng/ml group (27.05%) showed significantly higher rate than 10, 500 ng/ml, and control group (23.45%, 17.99%, and 19.68%, respectively, p<0.05). We also evaluated the effects of different NEAA and leptin treatment time on the development of porcine embryos after ICSI. Among groups of embryos cultured in the presence of NEAA or leptin for whole 7 days (D 1-7), first 4 days (D 1-4), the subsequent 3 days (D 5-7), both NEAA (27.13%, 21.17 %, and 17.56%, respectively, p<0.05) and leptin (25.60%, 20.61%, and 16.53%, respectively, p<0.05) showed that supplementation for whole 7 days significantly increased the blastocyst formation rate compared with the other groups of D1-4 and D5-7. We further evaluated the combination effect of 1% NEAA and 100 ng/ml leptin compared with the effect of each supplementation with 1% NEAA or 100 ng/ml leptin or no supplementation on development of embryos. For blastocyst formation, combination group of NEAA and leptin (24.78%) showed significantly higher rate than other three groups (18.37%, 20.44 %, and 13.27%, respectively, p<0.05). We further evaluated the expression of proapoptosis genes such as BAX and BAK and anti-apoptosis genes, BCL-XL and BCL-2 in blastocysts cultured in the presence of 100 ng/ml leptin. RT-PCR analysis revealed that leptin supplementation significantly decreased the expression of pro-apoptosis genes as well as increased the expression of anti-apoptosis genes. These results of present study demonstrate that NEAA and leptin could improve the in vitro development of ICSI- derived porcine embryos with optimal concentration of each reagent. Furthermore, the optimal culture condition could increase the quality of ICSI-derived embryos in vitro.

본 연구는 쌀을 다량 첨가하여 양조한 맥주의 품질개선을 위하여 아밀라아제, 프로테아제, 쌀에 결핍된 아미노산의 첨가를 시도하여 그 품질 개선의 효과를 분석하였다. 품질 요소는 당화액인 wort의 당도, 환원당, pH, 점도의 변화를 측정하였고, 최종 제품인 맥주의 알코올 함량, 거품 안정성, 쓴맛, 색도의 변화를 측정하였다. 실험에는 200 mesh의 쌀가루를 맥아와 쌀가루의 총량에 대하여 0, 40, 50, 60, 70%를 첨가하였다. 실험 결과 쌀 함량이 증가함에 따라 wort의 당도, 환원당 및 점도는 감소하였고, pH는 증가하여 당화력이 떨어졌다. 그러나 아밀라아제 첨가에 의하여 당화력이 다시 회복되었기 때문에 아밀라아제 첨가로 쌀맥주 제조 및 제품의 성능이 향상되는 결과를 보였다. 쌀 함량 증가에 따라 맥주의 알코올 함량 및 거품 안정성은 감소하였고, 쓴맛과 노란색이 증가하였다. 그러나 프로테아제 첨가에 의하여 알코올 함량, 쓴맛 및 노란색은 다시 회복되었으나 거품 안정성은 더욱 감소하였다. 글루타민산의 첨가에 의하여 노란색 외의 성질은 모두 회복되었다. 그러나 라이신 및 프롤린의 첨가에 의하여 알코올 함량 및 쓴맛은 회복되었으나 거품 안전성이 낮아지고, 색이 비정상적으로 탁하게 나타났다. 결과적으로 쌀 첨가 맥주의 품질향상 인자는 아밀라아제의 첨가, 글루타민산의 첨가, 프로테아제의 첨가의 순으로 결정되었다.

Squid ink was added to the low salt fermented squid by 4% of concentration and ripened at 10℃ for 6 weeks and at 20℃ for 28 days. The effect of the squid ink on the titrable acidity and free amino acids of low salt fermented squid were investigated. The results are as follows; The titrable acidity in the salt fermented squid without addition of the squid ink was continuously decreased except for the salt fermented squid with 9% salt content till the latter stage of the ripening, had larger decreasing range than treatment groups. Seeing the composition of free amino acid, the major amino acids are proline, arginine, glutamic acid. leucine and glycine.

This study was carried out to investigate the effect of muscle part and aging period on free amino acids and aroma compounds of Hanwoo (Korean cattle) cow beef. The M. longissimus (ML) and M. semitendinosus (MS) from 101 mon-old-cows were aged at 2℃ for 14 d. The free amino acids concentration increased in both ML and MS on 14 d of aging. In ML, asparagine, glutamine, histidine, glycine, threonine, arginine, tyrosine, phenylalanine, isoleucine, leucine and lysine were significantly (p<0.05) higher than those in MS. Varieties of aldehydes, ketones, alcohols, hydrocarbons, nitrogen and sulfur compounds were detected in both ML and MS and majority of these compounds showed increasing trend on aging. The ML had higher 14 aldehydes (acetaldehyde, 2-methylpropanal, 3-methylbutanal, 2- methylbutanal, pentanal, hexanal, heptanal, E-2-heptanal, octanal, 2-octenal, 2-nonenal, E-2-decenal, E,E-2,4-decadienal and 2-undecenal), 5 ketones (2-propanone, 2,3-butanedione, 2-butanone, 2-heptanone and 2,3-octanedione), 4 alcohols (ethanol, 1-pentanol, 1-hexanol and 1-octanol), 3 hydrocarbons (3-ethyl-2-methyl-1,3-hexadiene, 3- methyldecane and 2,2-dimethyloctane) significantly (p<0.05) compared with MS. However, the MS had higher 5 nitrogen and sulfur compounds (methanethiol, dimethyldisulfide, fufural, 2,5-dimethylpyrazine and 2-octylfuran) significantly (p<0.05) compared with ML.

This study was carried out to investigate the effect of amino acids complex and choline supplementation on the antioxidant enzyme activities and oxidative stability of Hanwoo (Korean cattle) beef. Fifteen months-old-Hanwoo steers were assigned into two groups and fed on a basal diets with or without amino acids complex (≥25% L-lysine monohydrochloride+≥8% DL- methionine)+choline (≥25% choline chloride) for 12 months. After slaughter, the M. longissimus from carcasses were stored at 4℃ for 7 days. Catalase, glutathione peroxidase and total superoxide dismutase activities were found to be unaffected by supplementation of amino acids+choline. After 2 days of storage, significant decline (p<0.05) in lipid oxidation (2-thiobarbituric acid reactive substances, TBARS) was observed when supplemented with amino acids+choline. However, supplementation of amino acids+choline maintained meat color as indicated by higher CIE L* (Lightness), a* (Redness), b* (Yellowness) and C* (Chroma) values during storage. It was therefore concluded that supplemental amino acids+choline could stabilize the lipid oxidation stability and meat color in Hanwoo beef.

Glycoprotein hormones have a common α-subunit that is involved in the signaling pathway together with G protein, adenylcyclase and cAMP induction; however, it is an unclear how this common structure is related to hormonal action. To determine the biological functions of the COOH-terminal amino acids in the α-subunit of these glycoprotein hormones, a tethered-molecule was constructed by fusing the NH2-terminus of the α-subunit to the COOH-terminus of the β-subunit of equine chorionic gonadotropin (eCG). The following deletion mutants were created by PCR; Ile was inserted at position 96 to form Δ96, Lys was substituted at position 95 to form Δ95, His was inserted at position 93 to form Δ93 and Tyr was substituted at position 87 to form Δ87. Each mutant was transfected into CHO-K1 cells. Tethered-wt eCG, and Δ96, Δ95, and Δ93 mutants were efficiently secreted into the medium but the Δ87 mutant was not secreted. Interestingly, the RT-PCR, real-time PCR, and northern blot analyses confirmed that the RNA was transcribed in the Δ87 mutant. However, the Δ87 mutant protein was not detected in the medium or the intracellular fraction of the cell lysates. The LH- and FSH-like activities of the recombinant proteins were assayed in terms of cAMP production using rat LH/CG and rat FSH receptors. The metabolic clearance rate (MCR) was determined by injecting rec-eCG (2 IU) into the tail vein. The Δ95 and Δ93 mutants were completely inactive in both the LH- and FSH-like activity assays. The Δ96 mutant showed slight activity in the LH-like activity assay. In comparison to the wild type, the activity of the Δ96 mutant in the FSH-like activity assay was the highest among all the mutants. The MCR assay in which rec-eCG was injected showed a peak at 10 min in all the treatment groups, which disappeared 4 h after injection. These results imply a direct interaction between the receptor and the COOH-terminal region of the α-subunit. The data also reveal a significant difference in the mechanism by which the eCG hormone interacts with the rLH and rFSH receptors. The COOH-terminal region of the α-subunit is very important for the secretion and functioning of this hormone.

This study was carried out to investigate amino acid contents of golden mushroom and pink mushroom. The amino acid analysis was followed by AccQ-Tag method and HPLC on gradient conditions. Seventeen amino acids were analyzed and sixteen amino acids were found in golden mushroom ; fifteen amino acids in pink mushroom respectively. Among total amino acid in golden mushroom, cystein content was the highest and glycine, glutamic acid, proline were followed. Among total amino acid in pink mushroom, cystein was the highest and glycine, lysine, methionine were followed. As shown in Fig.1, 2, concerning amino acids, cystein was dominant. and alanine was detected in golden mushroom and Pleurotus ostreatus sold in market but pink mushroom. Phenylalanine was detected in Pleurotus ostreatus sold in market but that was not detected in golden mushroom and pink mushroom.

This study was designed to investigate the effect of essential amino acids (EAA) and/or non-essential amino acids (NEAA) on the development of parthenogenetic and somatic cell nuclear transfer (SCNT) porcine embryos in vitro. To evaluate the timing of amino acids supplementation, activated oocytes were cultured in NCSU23-PVA with EAA, NEAA or NEAA+EAA (AAs) during specific periods as below: EAA, NEAA or AAs were supplemented during Day 0 to 6 (whole culture period: ALL), Day 2 to Day 6 (post-maternal embryonic transition period: POST-MET), Day 5 to Day 6 (post-compaction period: POST-CMP), Day 0 to Day 2 (pre-maternal embryonic transition period: PRE-MET), or Day 0 to Day 4 (post-compaction period: PRE-CMP). Supplementation of NEAA decreased cleavage rates in PRE-MET and PRE-CMP and also decreased blastocyst rates in POST-CMP. On the other hand, EAA significantly enhanced blastocyst formation rate in POST-MET and no detrimental effect on embryonic development in other groups. Interestingly, NEAA and EAA had synergistic effect when they were supplemented to the medium during whole culture period. Supplementation of AAs also enhanced SCNT porcine embryo development whereas BSA-free medium without AAs could not supported blastocyst formation of SCNT embryos. In conclusion, existence of EAA and NEAA in defined culture medium variously influences the development of parthenogenetic and SCNT porcine embryos, and their positive effect are only occurred when both EAA and NEAA are supplemented to the medium during whole culture period. Additionally, AAs supplementation enhances the blastocyst formation of SCNT porcine embryos when they are cultured in the defined condition.

This study was carried out to investigate amino acid contents of golden mushroom and pink mushroom. The amino acid analysis was followed by AccQ-Tag method and HPLC on gradient conditions. Seventeen amino acids were analyzed and sixteen amino acids were found in golden mushroom ; fifteen amino acids in pink mushroom respectively. Among total amino acid in golden mushroom, cystein content was the highest and glycine, glutamic acid, proline were followed. Among total amino acid in pink mushroom, cystein was the highest and glycine, lysine, methionine were followed. As shown in Fig.1, 2, concerning amino acids, cystein was dominant. and alanine was detectied in golden mushroom but pink mushroom.

본 연구는 아미노산의 첨가가 돼지 수정란의 체외 발달율에 미치는 영향을 구명하고자 PEF가 함유된 NCSU-23을 기본배지로 체외성숙 및 체외배양액을 조성한 후 EA(Essential amino acid), NA(Non-essential amino acid) 및 EANA(EA+ NA)를 첨가하여 체외성숙, 체외수정 및 체외발달에 미치는 영향을 조사하였다. 체외성숙 배지에 아미노산을 첨가한 결과 MH 단계까지의 체외성숙율은 NA 첨가군이 83.3%로 대조구 70.0%에 비하여 유의적으로(p<0.05) 높았다. 그러나 체외 수정 이후의 배 발달율과 수정율에서는 아미노산 첨가군과 무첨가군 사이에 유의적인 차이는 없었다. 체외배양액에 아미노산을 첨가한 후 배반포의 내부세포괴(ICM) 세포와 영양배엽(TE) 세포의 발달에 미치는 영향을 조사한 결과, ICM에서는 유의차를 발견할 수 없었으나 TE 세포는 EANA 처리구가 18.0±0.5개로 대조구 16.09±0.56개에 비해 유의적(p<0.05)으로 많았다. 총세포수에서도 EANA 처리구가 50.0±1.0개로 대조구 44.2±1.1개보다 유의적(p<0.05)으로 많았다. 이상의 결과를 종합할 때, 돼지의 체외수정란 생상에 있어서 아미노산의 첨가는 배반포로의 발달율에는 영향을 미치지 못하였으나 체외성숙율을 높이고 배반포의 세포수 향상에 도움을 주는 것으로 판단된다. 특히, 영양배엽(W) 세포의 발달율이 높은 것으로 보아 아미노산의 첨가는 돼지수정란의 착상에 도움을 줄 것이라 기대된다.