Low water activity foods usually do not support growth of pathogens. However, in recent years, a number of outbreaks associated with low water activity foods occurred, making people have a new understanding of microorganisms about low water activity foods and resulting in the safety of these foods becoming a major concern. Traditional thermal sterilization methods are difficult to be applied in low water activity foods because of its poor heat transfer and strong heat resistance of microorganisms. Radio frequency (RF) is considered as one of the most potential technologies in food industry and has an excellent prospect on content of the advantages of rapid and uniform heating. The Principle of RF-Sterilization is similar to Microwave, but RF has lower frequency and wider wavelength, therefore RF penetration depth is more suitable for Sterilization. For the pre-packaged food, there is few report on the effect of RF on the food and packaging materials. This presentation aims to discuss the influence of RF through comparing to Microwave.

Due to ever increasing trends in food safety, food manufacturers should take sanitary/ hygienic processing/packaging into key consideration. Minimizing the attachment of spoilage and pathogenic organisms to food contact surfaces and packaging materials is one of the major challenges in the fields of food science and biosafety. The bacteria adhering to the surface can produce subsequent hard-to-remove biofilms that potentially cause cross-contamination of processed foods. The contamination of spoilage to the food leads to shorten product shelf-life and, if foodborne pathogens are present, the consumption of contaminated food may possess a health hazard to consumers. In particular, a variety of surfaces in food packaging are vulnerable to development of biofilms as bacterial community is formed by adhering pathogens from native microflora in raw materialsfor prolonged contact time. It is now recognized that 80% of food outbreaks are related to biofilms. Therefore, inhibition of initial adhesion of biofilm-forming pathogenic cells on food contact surface is critically needed to minimize foodborne pathogen outbreaks.Bacterial adhesion is highly influenced by the substratum topography, i.e. roughness and porosity. Recent advancements in fabrication have made it possible to create well-organized nanofeatures (i.e. nanoporous and nanopillared) uniformly over a large surface area of a metal specimen. Nanosmooth (control) and nanoporous stainless steel foil surfaces were fabricated by precisely anodizing the degreased specimen in a 5% vol. of perchloric acid in anhydrous ethylene glycol. The applied voltage and anodization time were varied to obtain different pore diameters. The presences of 50 and 80 nm nanoporous patterns significantly inhibited the adhesion of L. monocytogenes by 2.0 to 2.3 log-cycles, depending on the pore diameters. It was found that nanoscale surface patterning and treatments are capable of enabling precise controls of molecular, physical, and biochemical interactions that govern bacterial adhesion to the solid substratum.

Antioxidant packaging have been investigated for a very long time, since lipid oxidation may cause a lot of quality problems which are not just on lipids but also to other components of food during storage and transportation. Lipid oxidation of packaged food can be by partly excluded by adding antioxidants to the packaged food, however, in order to make sure sustain release of antioxidant in a relatively long term and avoid food safety problems concerned by consumers, controlled release antioxidant active packaging, on the basis of active packaging, has been introduced and investigated. Controlled release packaging (CRP) means that active compounds release from packaging materials in a controlled manner which offers a prolonged delivery of active compounds at predictable and reproducible release rate. This study focuses on three subject in regard to the realized methods of controlled release of antioxidants in food packaging, which include encapsulation of active compounds with a mesoporous matrix, modification of structure and composition of multilayer film, and preparation of hydrogel bio-based packaging polymers.

Food quality indicator is a simple device to communicate information of food quality to consumers. Change of specific gas composition in the headspace of food packaging can be considered as a food quality indication. For examples, level of carbon dioxide (CO2) increases in the headspace of packaged kimchi which is fermented over time during storage. The elevated ammonia (NH3) level inside a food package is an indication of spoilage. In this study, CO2 indicators and NH3 indicators were developed to monitor food quality and their performance were evaluated. Especially, the correlation between the food quality change and the indicator change were studied during storage. Two types (chitosan-based and whey protein isolate-based) of CO2 indicators was developed to monitor the CO2 concentration in the headspace of a food package. The CO2 indicators showed a visual change depending on CO2 level in the package headspace and were installed on the packaged kimchi and the packaged makgeolli in order to notify their ripening stage. NH3 indicators were developed to monitor the headspace NH3 concentration in the packaged fishery products such as fermented skate. The NH3 indicators showed a prominent color change depending on NH3 level as the skate was fermented over time. In addition, the polylactic acid (PLA)-based package box installing NH3 indicator was developed for the fermented skate and the color change of the indicator was evaluated at different storage temperatures over time. In conclusion, the gas indicators incorporated into the package was able to simply inform food quality over time.

With the development of marketing, time-temperature indicator (TTI) is applied to monitor the storage temperature and time to predict the shelf-life of food by irreversible color change. This study mainly focused on developing the solid-type enzymatic time temperature indicator as a first exploration on printable TTI. It was based on the phenomenon that starch would change its color into blue when meets iodine, and the hydrolysis of enzyme make the color turn to colorless gradually. Combining the microencapsulation technology to immobilize enzyme, several TTIs with different formulas were achieved, and hopefully to be applied on different foods, whose storage temperature is 4 and their shelf-life could be 5-6 days. Chilled pork meat was chosen as an indicating target to figure out a suitable specific TTI by activation energy comparing and lasting time matching.

Aims: The objective of this study was to demonstrate the possible use of the psycrhotrophic, acid producing, and non-pathogenic microflora of the target chilled food (e.g. imitation crab sticks, ICSs) as time-temperature integrator-based materials for a quality indicator of the food. Methods and Results: Three strains out of four hundred and twelve psychrotrophic LAB colonies isolated from the ICSs were selected and identified as the genus Weissella (16S rDNA gene sequences), Gram-positive, catalase-negative, acid producing, and not hemolytic. Three prototype TTIs were correspondingly developed. A modified imitation crab medium was used as the bacterial nutrient source. The color change of a particular TTI in response to the experimental static and dynamic temperature conditions well represented the microbial growth and spoilage points of the Pseudomonas spp. and LAB which were justified as the spoilage microorganisms of thirty-three commercial ICSs. Conclusions: The psychrotrophic LAB based TTI is likely to be an effective tool for monitoring ICSs shelf life during storage and distribution. Significance and Impact of the Study: This finding suggests that the indigenous microflora of target food can be considered as potential materials for customizing a new TTI particularly for the target food. The food analog is potentially used as the microbial medium.

The purpose of this study was to analyze University student’s dietary behavior for processed foods and recognition on food nutrition labelling according to the degree of health concern. For this study, 302 University students in Gyeonggi region was surveyed by questionnaire from May in 2016 and it included the questions about general matters, dietary behavior on processed foods, and the food nutrition label system. It was analysed by SPSS win 23.0 program. And the results are as follows. According to the result of the factor analysis, the preference of processed foods were showed 3.21~3.97. There were significant differences in milk and milk products preference according to health concern (p<0.05). Also frequency of processed foods was shown significant differences in milk and milk products, noodles, beverage, and fast food (p<0.05), whereas no significant differences were found in meat products, snack, bread and frozen food (p>0.05). Also, the processed food purchase consideration according to health concern was shown significant differences in nutrition, origin, manufacturing company (p<0.05), whereas no significant differences were found in taste, price, exterior, brand, and shelf life (p>0.05). Thus, it is necessary to do continuing public relations and to provide more opportunities for nutrition education so that the University students may obtain right dietary behavior on processed foods and the system of food nutrition indication be established properly.

This study examines the effects of consumer beliefs for food certifications on the behavioral intentions and the behavioral intention biases to purchase the certified seafoods by a subjective probability model which is on the basis of the mathematical probability model and the covariance model. The food certifications used on this study are‘Organic foods’,‘Traceability system of food products’and ‘. HACCP’. The representative foods of fishery products on this study is seasoned laver. The current study showed the following results. First, consumers have more than two different beliefs each for all certifications which are the subjects of this study. The beliefs of the certifications have an impact on the consumers when they consider to buy the certified seafood products. Second, consumers try to persuade by themselves to ensure that their particular belief about the certification could lead to a purchase the seafood products. Consumer beliefs of the“environmentally friendly production”on the organic foods certification is an important factor as much as the“guarantee of food safety”belief making a positive purchasing behavior intentions(PBI) bias for the organic seafood products. Consumers also have a positive PBI bias for certified seafood products in all certifications as long as a certification is considered to “guarantee the transparency of the food distribution process”as its belief.‘Traceability system’was the only one which didn’t generate a positive PBI bias from the belief of“guarantee of food safety”out of three certifications.

The purpose of this study was to determine the optimal mixing ratio of olive oil, parmesan cheese, and nuts for preparation of pesto with spinach. Based on a surface response methodology (RSM), the independent variables were olive oil (100~300 g), parmesan cheese (25~75 g) and nuts (12~36 g). The dependent variables were physico-chemical properties as pH, color values (L, a, and b values), viscosity and sensory evaluation. pH decreased with increasing parmesan cheese levels. The L value decreased and the a and b values increased with increasing olive oil levels. Viscosity was negatively correlated with olive oil levels but positively correlated with the amounts of parmesan cheese and nuts. The addition of oil exerted a positive effect on gloss, and oily taste. In the sensory evaluation, the values of appearance, color, flavor, taste and overall acceptance were in the range of 4.4~7.2, 3.7~7.4, 4.4~7.1, 3.9~7.3 and 3.5~7.6, respectively. The sensory evaluation results showed significant values in appearance (p<0.01), flavor (p<0.01), taste (p<0.01) and overall acceptance (p<0.05). The optimal amounts of spinach pesto with olive oil, parmesan cheese, and nuts were determined by numerical optimization of a canonical model and graphical optimization. The optimal amounts were 175.29 g of olive oil, 49.51 g parmesan cheese, and 27.37 g of nuts per 100 g of spinach.

The objective of the current study was to compare the execution of hygiene and safety management according to the characteristics of children’s food service stations, with the ultimate objective of providing sanitary and safe food service to children. To this end, the study conducted surveys of 73 public and private pre-schools with fewer than 100 children in district A from July 30 to October 31 2015. The differences were evaluated by t-test and analysis of variance. The confidence and validity of the results were analyzed by six food ingredient and facilities management questions, three personal hygiene and facilities management questions, four environmental management questions, and five storage and treatment management questions; for a total of 18 questions. The cumulative distribution of the four factors was 61.569%, and the Cronbach’s alpha was 0.821, which indicated that the results were reliable. The results revealed that public pre-schools (a) and home-based pre-schools (b) had different levels of food ingredient and facility management, but no differences in the number of cooks, number of children served, type of food distribution, and the existence of mass food service facilities. The results of the study may be used to develop hygiene and safety management manual and educational contents to promote the health of the children served.

This study was conducted to evaluate the quality of barley (Huinchalssalbori) and domestic wheats (Keumkangmil, Baegjoongmil, Jogyeongmil). The pH and total acidity of mixed Makgeolli were 4.04~4.12% and 0.94~1.06%, respectively. The total acidity, sugar and alcohol contents of Makgeolli, but not pH, varied significantly by wheat cultivar (p<0.05). In terms of color values, the L-value of Baegjoongmil, a-value and b-value of Keumkangmil were highest. The reducing sugar contents was approximately 5.65~7.85 mg/mL, and those of Jogyeongmil and imported wheat were approximately 5.70 mg/mL lower. The yeast cell numbers did not differ significantly, with the exception of in the rice Makgeolli (p<0.05). Among the organic acids (citric, malic, pyruvic and lactic acids) in Makgeolli, citric acid was present at the highest concentration. Regarding the sensory characteristics of Makgeolli mixed with barley and wheat, taste and overall acceptability were highest in Baegjoongmil, and appearance and flavor were highest in Keumkangmil. The rice Makgeolli showed the lowest sensory values, with the exception of appearance. The results of this study suggest that mixing Makgeolli with barley and wheat is an expected to replace the wheat materials in the domestic wheat to be imported.

This study was performed to develop and evaluate beverage prepared with optimally roasted seoritae to maximize the isoflavone content and antioxidant activities of the beverage. Isoflavone content was maximized at the roasting temperature of 110℃ for 20 min. Both DPPH radical scavenging activity and ABTS radical scavenging activity along with total polyphenol content were highest when seoritae was roasted at 110℃ for 20 min. Western blotting was used to determine the level of nuclear factor of activated T-cells 1(NFATc1) involved in controlling osteoclast differentiation. The results showed that NFATc1 had a concentration-dependent inhibitory effect when the RoS110 (roasted seoritae at 110℃ for 20 min) samples were processed at varying concentrations (10, 50, and 100 μg/mL). Tea samples were prepared from optimally roasted seoritae by varying brewing times (5~90 min) at 65℃, and tea brewed for 60 min had the highest preference with 65℃ as the preferred temperature for drinking.

연산 오계육은 오래전부터 건강기능 증진 및 치료 효능이 높은 것으로 알려져 왔다. 최근 천 연물 단백질 유래 기능성 펩타이드 효능이 알려짐에 따라, 본 연구는 연산오계 부산물인 내장육 단백질 로부터 고압처리기술과 프로티아제를 이용하여 펩타이드 생산 최적공정과 생성물의 특성을 연구하였다. 내장육의 가수분해는 효소 bromelain 과 내장육을 고압 반응기에 투입을 하여 실시하였다. 최적 공정 조건 확립을 위하여 고압처리기의 압력(30 - 100 MPa), 효소반응 시간 (1 - 5시간), 내장육의 양(10 – 30%)의 범위에서 수행되었다. 효소 반응 후 각 조건에 따른 내장육 단백질의 가수분해도, 생산 펩타이 드들의 아미노산 및 분자량 분포를 분석하였다. 연구 결과 내장육 단백질 가수분해 최적조건으로 압력 90 MPa, 효소반응시간 3–4시간, 내장육의 함량 20%에서 결정 되었다. 최적조건에서 오계 내장육 단백 질의 65% 이상이 가수분해 되었다. 대부분의 가수분해물의 분자량들은 400–1,000 Da 이하의 분포를 보여주어 대부분이 펩타이드로 판단되었다. 생산 펩타이드들은 비극성 소수성 아미노산들 42.3%, 극성 비전하 아미노산들 26.0%, 양 전하 아미노산들 13.3%, 음 전하 아미노산들 18.6% 로 분포되었다. 따라 서 항산화 능력이 뛰어난 비극성 아미노산의 분포를 보아 건강 기능 식품 소재로서 활용할 가치가 높을 것으로 기대를 한다.

The purpose of this study was to investigate the relationship between frequency of coffee consumption, metabolic biomarkers, and nutrition intake in adult participants in the combined 2007~2009 Korean National Health and Nutrition Examination Survey (KNHANES). Subjects (2,095 males and 3,297 females) were classified according to sex and frequency of coffee consumption (≤1 time/month, ≥2 times/month and ≤6 times/week, 1 time/day, 2 times/day, 3 times/day) using food frequency questionnaires. Nutrition intake was analyzed using 24 h recall data. The 3 times/day coffee consumption group had a significantly higher age, and frequency of smokers and drinkers compared to the ≤1 time/month coffee consumption group in both male and female participants. Males in the 3 times/day coffee consumption group had a significantly lower HDL-cholesterol level, but females had a higher waist circumference compared with the ≤1 time/month coffee consumption group. Males in the 3 times/day coffee consumption group had a significantly lower nutrient density of fiber, vitamin B2, vitamin C, calcium and phosphorus compared with the ≤1 time/month coffee intake group. Females in the 3 times/day coffee consumption group had a significantly higher nutrient density of fat and niacin, but lower nutrient density of carbohydrate, calcium, phosphorus, and iron compared with the ≤1 time/month coffee intake group. In males, the frequency of coffee consumption was not associated with the levels of metabolic biomarkers. In females, the frequency of coffee consumption was positively associated with diastolic blood pressure after adjustments for multiple confounding factors, including age, BMI, smoking status, alcohol consumption, physical activity and energy intake. Coffee consumption was associated with decreased diastolic blood pressure in females. These findings suggest the importance of an awareness of the association between coffee consumption and metabolic risk.

This study was carried out to evaluate the quality characteristics of Korean domestic commercial white wines. Four sample wines were analyzed in terms of their pH, total acidity, volatile acidity, alcohol content, soluble solid, free SO2, total SO2, color, free sugar content, organic acid, volatile compounds, total polyphenol content, DPPH radical scavenging activity and sensory characteristics. The pH of wines ranged from 3.06 to 3.76 and their total acidity from 0.43 to 0.83% (v/v). The alcohol content of samples ranged from 9.4 to 12.8% (v/v) and their soluble solid contents from 9.9 to 12.3 °Brix. The free SO2 and total SO2 contents of the W1 wine were lower than those of the other wines, while the color intensity, a (redness) and b (yellowness) of W1 wine were higher than the values of the other wines. The malic acid contents of the four wines indicated that complete malolactic fermentation (MLF) occurred in W1, incomplete MLF in W3, and was absent in W2 and W4. Aroma analysis resulted in identification of 10 alcohols, 11 esters, 2 ketones and 6 miscellaneous compounds. 2,3-Epoxybutane and 1-(ethenyloxy)-pentane levels in were significantly higher W1 than the other wines, while several types of ester predominated in W2, W3 and W4. W1 had the lowest sensory score among the four wines. Therefore, the quality characteristics of domestic white wines are considered to be acceptable.

This study was conducted to investigate changes in the proximate composition, antioxidant activities, and α-glucosidase inhibitory activity of Opuntia ficus-indica (OFI) cladodes cultivated in Jeju (JJ1, JJ2, JJ3) and Jeonnam (JN1, JN2). The difference in the proximate composition (crude protein, lipid and ash content) of OFI between the two regions was not significant. Ca, Mg and Na were the major mineral components of OFI. The ascorbic acid content of OFI ranged from 57.87 to 143.72 mg/100 g. A 70% ethanol extract was used to investigate the antioxidant content and activity as well as the α-glucosidase inhibitory activity. The total polyphenol and flavonoid contents of OFI were 38.69~55.29 and 3.33~4.03 mg/g, respectively. The antioxidant activities based on the DPPH and ABTS free radical scavenging assays were 45.19~ 61.52% and 39.15~51.96%, respectively, at a concentration of 1 mg/mL. The inhibitory activity of OFI extracts against rat intestinal α-glucosidase was 29.72~45.73% at 1 mg/mL concentration, and JN1 showed the highest α-glucosidase inhibitory activity. This information could be very useful for authentication of Opuntia species with the highest potential as sources of nutritional and therapeutic elements.

Recently, the incidence of cardiovascular diseases including high blood pressure and heart disease has increased with increased meat consumption in Korea. This study was performed to determine the optimal mixing ratio among ratios of 0, 1:0, 0:1, 1:1, 2:1, and 1:2 of mulberry leaf powder to mulberry fruit powder based on the anti-inflammatory effects. Then, the quality characteristics of the sauce for meat prepared with different mixing ratios (0, 1:1, 1:2, 1:3, 1:4, and 1:5) of mulberry leaf powder to mulberry fruit powder were assessed. The inhibitory effects of ML2MF1 (2:1 ratio of mulberry leaf powder to mulberry fruit powder) on NO and TNF-α production were superior to those of other mixing ratios. With respect to color values of the sauce for meat prepared with different mixing ratio of mulberry leaf powder to mulberry fruit powder, as the mulberry fruit powder mixing ratios was increased, redness was increased but lightness and yellowness were decreased. The sensory evaluation of ML1MF5S (1:5 ratio of mulberry leaf powder to mulberry fruit powder) sauce for meat showed the highest score with respect to color, smell, taste, harmony with meat and the overall preference. In conclusion, as the mulberry leaf powder mixing ratio was increased, the anti-inflammatory activities were increased. But sensory evaluation scores were increased as the mulberry fruit powder mixing ratio was increased. Therefore, by performing further study, the method to increase mulberry leaf powder in the mixing ratio for improving the sensory evaluation should be provided.

Bitter melon (Momordica charantia) is used in traditional herbal medicine in many Asian countries for the treatment of several diseases such as diabetes, eczema, night blindness, psoriasis, and rheumatism. Especially, most reports concerning the biological activities of bitter melon have focused on its effects on diabetes and hyperglycemia. Also, bitter melon is regarded as a longevity food, suggesting that it has several beneficial effects on anti-aging and the maintenance of a healthy state. Thus, we investigated whether bitter melon could increase the capacity of exercise in this study. Interestingly, bitter melon fruit extract activated AMP-activated protein kinase (AMPK), which is important for regulating glucose homeostasis, mitochondrial content and exercise capacity. In addition, bitter melon extract increased the expression of enzymes involved in fatty acid oxidation such as mitochondrial uncoupling protein 3 (UCP3), carnitine palmitoyl transferase 1b (CPT1b), and pyruvate dehydrogenase lipoamide kinase isozyme 4 (PDK4). Moreover, exercise tolerance was much more enhanced in bitter melon treated animals compared to the non-treated control group. These results suggest that bitter melon is a promising candidate for the development of functional foods beneficial for physical strength and the enhancement of exercise capacity.

To improve the shelf-life of mushrooms, Lentinula edodes GNA01 mushrooms were treated with gel packs containing slow-released chlorine dioxide (ClO2) gas at 5~10 ppm for 5 days at 20℃ and the weight loss rate as well as the changes in pH, color and texture properties of the treated samples were investigated. The weight of the control and ClO2 gas treated samples were decreased slightly, and there were no differences during the storage period. However, the weight of the control changed faster than those of the ClO2 gas treated samples during storage period. The pH in the control and in the ClO2 gas treated samples were decreased during storage period, but the samples treated with 5 and 7 ppm ClO2 gas were the least changed. On the other hand, the samples treated with 10 ppm ClO2 gas showed no difference from the other treatments during 4 days, but the pH was lower than that of the control on the fifth day. The lightness of inside and outside in mushroom were decreased whereas redness and yellowness were increased during storage period. However, color changes in the ClO2 gas treated samples were lower than those of the control. Especially, the samples treated with 5 and 7 ppm ClO2 gas were the least changed. The texture of the mushroom were decreased consistently during storage period. The texture of the control changed faster than those of the ClO2 gas treatments during 5 days. Especially, the samples treated 5 ppm ClO2 gas were the least changed.

This study aimed to examine the health-related lifestyle habits and eating behaviors according to gender, ethnicity, and residence type of university students in Yanbian, China. Self-administered questionnaires were collected from 302 university students. Of the male students, 12.1% and 42.0% were in the underweight and overweight groups, respectively, and of the female subjects, 21.3% and 16.3%, were in those respective groups. More male than female students preferred the overweight body somatotype. In contrast, about 49.4% of female students were hoping to be underweight, and female students had more obvious difficulties with body somatotype perception, whereas their exercise frequency and time spent exercising per day were much less than those of male students. More Chinese than Korean-Chinese subjects exhibited regular eating habits, which included eating at the same time everyday and at the same frequency per day; these habits were, accompanied by generally healthier lifestyle habits regarding regularity of activity and exercise time. Self-boarding students had a significantly higher BMI (23.7±5.1 kg/m2) and were more likely to be overweight (43.5%) as compared to students who lived in a dormitory or with family. Salty taste was preferred by Chinese students more than Korean-Chinese students, and greasy taste was preferred by Korean-Chinese students as compared with Chinese students. This study found that inherent and environmental factors are related with the dietary behaviors of university students in Yanbian, China. Further studies are required to elucidate the structural elements of family life and the sociocultural factors associated with dietary behaviors in Yanbian.