미국인이 즐겨먹는 기능성 고추케찹과 한국산 고추케찹의 생리활성 특성을 비교하고, 한국산 고추케찹의 외국인 관능 평가를 진행하여 고추케찹의 특성과의 상관관계를 분석하여 고부가 가치 고추가공품의 기초자료를 마련하고자 연구를 진행하였다. 미국산 고추케찹 4종과 한국산 고추케찹의 총 당, 염도, 당도의 이화학적 분석과 ASTA 값, 총 캡사이시노이드, 총 카로티노이드, 총 페놀, 총 항산화력의 생리활성 성분을 분석하고, 관능평가를 실시하였다. 한국산 고추케찹은 미국 고추케찹보다 생리활성 성분이 모두 높게 나타났으며, 관능평가 결과, 미국 고추케찹에 비하여 단맛과 짠맛이 약하고, 종합적 기호도가 높은 것으로 평가되었다. 생리활성 성분과 관능평가 결과의 상관관계 분석을 통하여 ASTA 값, 총 캡사이시노이드, 총 카로티노이드 함량과는 통계적으로 서로 양(positive)의 상관관계를 갖는 것으로 분석되었고, 이 3가지 생리활성 성분은 종합적 기호도와 높은 상관관계가 있는 것으로 나타났다.

Platycodon grandiflorum have been used as a traditional remedy and food source. This study was performed to investigate the immunomodulating effects of Platycodon grandiflorum in mouse, using ex vivo experiments. Six to seven-week old mice were fed ad libitum on a chow diet, and water extract of Platycodon grandiflorum was orally administrated at two different concentractions (50 and 500 ㎎/㎏ B.W./day) every other day for four weeks. In ex vivo experiments, the highest proliferation of splenocytes and levels of cytokine (IL-1β, IL-6, TNF-α) production were observed in 500 ㎎/㎏ BW/day supplementation group for all three cytokines stimulated by LPS. In conclusion, this study suggests that Platycodon grandiflorum extracts may enhance the immune function by regulating the splenocytes proliferation and cytokine production capacity by activating macrophages in mice.

In this study, soybean is used to produce soymilk according to various extracting methods and heating time. Specifically, the soy slurry is being filtered before being heated, or heated before being filtered. Following that the soymilk produced is freeze-dried to be powdered, and then, the quality characteristics of the powdered soymilk are mutually compared to determine the applicability of various food additives. The freeze-dried soymilk powder shows 2.03~6.35% of moisture content, and in terms of unit quantity, retained more proteins, which suggests that the ratio of protein extraction is higher than any other nutrients. Accordingly, the protein coefficient is significantly higher in soymilk powder being heated and processed than in raw soybeans. In particular, protein coefficient is the highest in the soymilk which is heated for 20 minutes before being filtered (SHBF20). The longer the heating time was, the trypsin inhibitor (TI) tended to be far less active. Such an inactivation seems to be more apparent in the “SHAF” soymilk powder than “SHBF” soymilk powder. Because protein had to be denaturated by heating for soymilk, the nitrogen solubility index (NSI) of soymilk powder is decreased considerably, while the protein digestibility, water absorption, emulsification and foaming activity all increase. Oil absorption tends to decrease slightly. As discussed above, the soymilk heated for 10 minutes after being filtered (SHAF10) and the soymilk heated for 20 minutes before being filtered (SHBF20) show optimum processing conditions for soymilk powder.

The purpose of this study is to examine the relationships between body mass index (BMI), satisfaction of perceived somatotype, and eating disorder of 133 female college students from Gyeong-gi province. The average weight is 55.3±8.8kg, and the BMI is categorized into three groups: underweight, normal weight, and overweight. In terms of the satisfaction for perceived somatotype, 58.6% of the total respondents replied "unsatisfied," which consists of 14% of the underweight group, 37.6% of the normal weight group, and 6.8% of the overweight group. This reveals that the negative association between the satisfaction levels of perceived somatotype and BMI in the normal weight group is statistically significant (p<0.001). Among three eating disorder scores, the first factor, the drive for thinness, shows significant difference (p<0.005) among groups with 2.82 points in the underweight group, 3.38 points in the normal weight group, and 3.86 points in the overweight group; such result shows that there is a drive for being skinny even within the underweight group. Second, the satisfaction levels of perceived somatotype type shows significant dissatisfaction among all groups with 3.41 points in the underweight group, 4.15 points in the normal weight group and 4.73 points in the overweight group. Third, the bulimia degree reveals a significant difference within each group, 2.15 points in the underweight group, 2.33 points in the normal weight group, and 2.78 points in the overweight group. Overall, more than half of the female college students are dissatisfied with their body shapes despite the fact that they are under or within the normal weight. In addition, the drive for being skinny and dissatisfaction levels of perceived somatotypes appear to be significantly higher with higher BMI levels.

The following study is the result of herbal teas puffed at different temperatures between 140~220℃. Depending on treatment temperatures, the water contents decreased, while some carbonization occurred and crude ash contents relatively increased. Also, the crude protein and crude fat experienced little changes. B(α)P contents (0.16~0.17 ppb) showed little change according to treatment temperatures. From this result, the B(α)P content differed depending on the treatment temperature and raw materials. Solid elution rate figures of the herbal teas ranged from 0.27~0.45% (w/w) and the rate of solid elution increased along with higher puffing temperatures. The reason for the increase in solid elution rates is due to the breakage of cross bridges between the raw materials in the herbal tea which are carbohydrates, proteins, lipids and etc. after treatments of physical changes rather than chemical ones.

The purpose of this study is to compare the perceptions and needs for nutrition education between early childhood teachers and parents. This data will address the effective plans and suggest further nutrition education programs for early childhood education centers. This study was conducted with 200 early childhood teachers and 174 parents in Busan. We collected data through questionnaires from teachers and parents and then computed the frequency and χ2 test to analyze the data. The questionnaire was revised and complemented on the basis of related literatures and previous researches. According to our results in this study, there was a significant difference between the teachers’ and parents’ perceptions on the need of nutrition education and the reason for needing nutrition education. And there were differences among aims, contents, methods, and effective ways of nutrition education for children. In addition, there was a significant difference among the needs from teachers and parents on nutrition education. Finally, the results of this study suggest various implications for developing nutrition education programs in early childhood education centers. These results can be used to develop nutrition education program by considering the teachers’ and parents’ perceptions and needs. These may be useful data when planning for the nutrition curriculum in early childhood education centers.

들기름 및 미강유를 베이스로 개발한 혼합 식용유는 오메가-3 지방산인 리놀렌산 함량이 약 20% 정도로 매우 높다. 이는 기존의 일반 식용유 중 오메가-3 지방 함량이 매우 높은 콩기름과 카놀라유의 리놀렌산 함량이 각각 6% 및 10% 내외인 점을 감안하면, 이들 식용유에 비하여 새로 들기름과 미강유를 혼합한 식용유는 2~3배 높은 비율로 오메가-3 지방산을 제공할 수 있어서, 오메가-3 지방산 섭취가 부족한 현대인의 식단에서 중요한 오메가-3 지방산 급원으로서의 역할을 할 수 있을 것이다. 뿐만 아니라, 토코페롤 함량도 약 46.77 ㎎/ 100 g oil 정도인데, 가장 주목할 일은 토코페롤 동족류 중 비타민 E의 활성이 가장 강한 알파-토코페롤(31.71 ㎎/100 g oil)이 가장 많이 존재하여, 총 토코페롤의 64%를 차지하고 있어서 비타민 E로서의 기능이 높다. 혈중 콜레스테롤 함량을 낮추는 기능성이 알려진 파이토스테롤 함량이 들기름과 미강유를 혼합한 식용유에 약 7,200 ppm 함유되어 있어, 일반적인 식용유의 3,000 ppm의 약 2.3배에 달한다. 본 연구에서 개발한 들기름과 미강유를 혼합한 식용유는 일반적인 식용유인 콩기름과 거의 유사한 산화안정성을 갖는 것으로 확인되어, 일반 들기름에 비하여 현저히 높은 산화안정성이 확보되었다.

국내 벼 품종 중 아밀로스 함량과 아밀로펙틴 조성이 다양한 품종의 막걸리 특성을 분석하여 막걸리 품질 고급화 및 다양화를 위한 자료를 제공하고자 본 실험을 수행한 결과는 다음과 같았다. ‘고아미2호’, ‘고아미3호’, ‘고아미4호’는 품종별 전분 특성과 호화 특성 분석 결과, 낮은 아밀로펙틴 단쇄 비율과 높은 아밀로스 함량에 기인하여 점성이 낮게 나타남을 확인할 수 있었고, 높은 호화개시온도로 인해 가열흡수비와 팽창용적이 각각 2.09~2.13, 25.02~26.78 ㎤로 다른 품종에 비해 유의하게 낮아, 막걸리 발효에 적합한 전분의 호화가 이루어지지 않음을 확인할 수 있어, 막걸리 제조를 위해 호화를 증진시키기 위한 가공 방법 연구가 필요할 것으로 판단되었다. 품종별 막걸리의 품질 특성은 ‘설갱’, ‘백진주’, ‘일품’의 알코올 함량은 17.3~18.0% 범위로 통계적인 유의차는 없었으나, ‘고아미2호’와 ‘고아미3호’, ‘고아미4호’는 10.0~10.5%로 상대적으로 낮은 알코올 함량을 나타내었고, 당도 또한 ‘설갱’, ‘백진주’, ‘일품’에 비해 절반 가까이 낮았으나, pH와 산도는 품종 간 유의한 차이를 나타내지 않았다. 또한 원료곡에서 높은 난소화성 다당류 함량을 지닌 ‘고아미4호’ 등은 막걸리에서도 역시 난소화성 다당류 함량이 유의하게 높아, 향후 막걸리 제품으로 이용 시 건강 기능성에 기여할 가능성이 높을 것으로 기대되었다. 결과적으로 아밀로펙틴 조성과 난소화성 다당류 함량에서 차이를 보인 ‘고아미2호’, ‘고아미3호’, ‘고아미4호’ 막걸리의 높은 제조수율과 난소화성 다당류 함량 및 낮은 알코올 함량과 당도를 확인하였으며, 지속적인 발효 공정 연구를 통해 국내 기능성 품종을 이용한 고품질 막걸리 개발이 필요할 것으로 판단된다.

생체 내 실험에서 발효 인삼꽃 추출물(FM), 발효하지 않은 인삼꽃 추출물(FD)과 대조군으로 생리식염수를 2주간 마우스 체중 ㎏ 당 100, 200 ㎎/㎏ B.W.의 농도로 마우스에 경구 투여한 후 LPS에 의해 활성화된 복강 대식세포가 분비하는 염증성 사이토카인 IL-6, TNF-α의 생성량을 측정하였다. 그 결과, IL-6는 발효 LPS로 자극한 경우, 두 가지 농도에서 모두 처리한 군에 비해 높은 증식능을 나타내었고, LPS로 자극한 결과, 특히 발효 인삼꽃 추출물 200 ㎎/㎏ B.W. 농도에서 유의적으로 낮은 IL-6 분비량을 보였다. TNF-α의 경우, 100 ㎎/㎏ B.W.와 200 ㎎/㎏ B.W. 두 농도 모두에서 LPS로 자극하지 않은 경우, 낮은 증식 효과를 보여주었고, 자극한 경우, 인삼꽃 시료를 첨가한 군이 대조군보다 낮은 TNF-α 분비량을 보였으며, FM에서 FD보다 더 TNF-α를 억제하는 효과가 큰 것을 볼 수 있었다. 이상의 결과에 따르면 FD의 사이토카인 IL-6, TNF-α 생성 효과는 200 ㎎/㎏ B.W. 농도 투여 시 효과적으로 면역 세포와 면역 기관의 주요 기능을 증진시킬 가능성이 있을 것으로 사료된다. 이러한 연구결과를 토대로 앞으로 인삼꽃 발효를 이용한 기능성 사료 개발과 더불어 산업적 측면에서 보다 긍정적인 효과를 얻을 수 있을 것으로 판단된다.

오렌지의 위생성 및 보존성을 향상시키고, 수입국의 검역 기준을 정립하기 위한 연구의 일환으로 오렌지에 0.4, 0.6, 0.8, 1, 1.5 kGy로 감마선 조사를 실시하여 3℃에서 60일 동안 저장하면서 미생물 및 이화학적 품질 특성을 조사하였다. 오렌지 1.5 kGy 조사구의 호기성세균, 효모 및 곰팡이는 각각 3.59 log CFU/g, 3.75 log CFU/g에서 1.75 log CFU/g, 2.26 log CFU/g까지 감소하여 조사선량과 저장기간에 따라 유의적인 차이를 보였다. pH는 비조사구와 조사구 사이의 유의적인 차이를 나타내지 않았고, 산도는 조사선량과 저장기간의 증가에 따라 감소하였다. Vitamin C 함량은 조사선량과 저장기간의 증가에 따라 유의적으로 감소하였다. 관능평가는 조사선량과 저장기간이 증가할수록 감소하였고, 1.5 kGy는 색깔, 신맛, 단맛, 향, 질감, 전체적인 선호도에서 가장 낮은 선호도를 보였다. 이상의 결과를 종합하여 볼 때, 감마선 조사는 미생물학적 안정성에는 효과적이지만, 1 kGy와 1.5 kGy는 물리적 특성과 관능 특성에 좋지 않은 영향을 끼치는 것으로 보고, 품질 변화를 최소화하기 위한 최적 선량은 0.4~0.6 kGy 인 것으로 사료된다.

헤미셀룰레이즈를 0.005%, 0.010%, 0.015%, 0.020%를 첨가한 자색고구마 제조한 식빵의 품질 특성과 관능검사를 실시하였다. 반죽의 pH와 총 산도는 시료들 간에 유의적인 차이가 없었다(p<0.05). 발효 시간에 따른 발효 팽창력은 발효시간이 길어질수록 유의적으로 증가하였고(p<0.05), 헤미셀룰레이즈 첨가량에 따른 발효 팽창력은 45분과 60분에서는 헤미셀룰레이즈 0.015% 첨가군이 가장 높았으며, 대조군이 가장 낮게 나타났다(p<0.05). 식빵의 pH는 대조군보다 헤미셀룰레이즈 첨가군들이 유의적인 차이가 없었으며(p<0.05), 총 산도는 헤미셀룰레이즈 첨가군들이 대조군보다 유의적으로 높았다(p<0.05). 비용적과 굽기 손실률은 헤미셀룰레이즈 첨가군들이 대조군보다 유의적으로 높았다(p<0.05). 수분 함량은 시료들 간에 유의적인 차이가 없었다(p<0.05). 명도는 대조군보다 헤미셀룰레이즈 첨가군들이 유의적으로 낮았고(p<0.05), 적색도는 0.005% 첨가군을 제외하고, 대조군보다 헤미셀룰레이즈 첨가군들이 유의적으로 낮았다(p<0.05). 황색도는 헤미셀룰레이즈 첨가군들이 대조군보다 다소 높았다(p<0.05). 견고성과 부서짐성은 헤미셀룰레이즈 첨가량이 증가할수록 유의적으로 감소하였으며(p<0.05), 복원성은 0.005% 첨가군을 제외하고, 헤미셀룰레이즈 첨가군들이 대조군보다 유의적으로 높았다(p<0.05). 소비자 기호도 검사는 색과 부드러움은 실험군들 간에 유의적인 차이를 나타내지 않았고(p<0.05), 향미는 헤미셀룰레이즈 첨가군들이 대조군보다 유의적으로 높았다(p<0.05). 종합적인 기호도는 헤미셀룰레이즈 0.010% 첨가군이 5.67로 가장 높은 값을 나타냈다. 이상의 실험에서 헤미셀룰레이즈 0.010% 첨가가 자색고구마 식빵의 부피를 개선하는데 최적 첨가량으로 확인되었다.

This study is conducted to investigate the effects of Chinese artichoke powder on the antioxidant activities and the quality characteristics of tofu. Tofus are being prepared with different amounts of Chinese artichoke powder in ratios of 0, 0.2, 0.4, 0.6 and 0.8% according to the soy milk quantity. According to the increasing contents of Chinese artichoke powder, the yield and turbidity of tofus are significantly increased (p<0.001) whereas the pH levels of tofus are significantly decreased (p<0.001). In terms of color, the L, a and b values are significantly decreased with increasing Chinese artichoke powder concentrations (p<0.001). From the texture profile analysis of the tofus, the chewiness, gumminess and cohesiveness of tofus are significantly decreased (p>0.01) according to the levels of added Chinese artichoke powder content. The contents of isoflavones such as daidzin and genistin are significantly increased with increasing concentrations of Chinese artichoke powder (p<0.001). The microstructure of tofus is being examined by SEM (Scanning Electron Microscope). In the sensory test, the sample which contains 0.4% of Chinese artichoke powder is ranked significantly higher (p>0.001) than the other groups according to several sensory parameters such as appearance, color, flavor, taste, texture and the overall preferences.

This study is performed to examine the functional and quality characteristics of glutinous barley Jeung-pyun added with beet powder. Jeung-pyun is being prepared with concentration of 0, 2, 4, 6 and 8% (w/w) of the beet according to nonglutinous rice powder weight. The pH level of Jeung-pyun batters decrease along with fermentation time. However, the pH level of Jeung-pyun is higher than that of the Jeung-pyun batter. For hunter’s color value of glutinous barley Jeung-pyun added with beet powder, the L (lightness) values decrease with increasing concentrations of beet powder, whereas a (redness) values increase (p<0.001). In the texture analysis, the hardness, chewiness and gumminess are significantly increased with the amount of glutinous barley Jeung-pyun with added beet powder. According to sensory evaluations, the appearance, color, flavor, taste, texture and overall quality are considered very good in the 4% of added beer powder in glutinous barley Jeung-pyun (p<0.001). The antioxidant activities of beet powder by DPPH free radical scavenging activity, ferric reducing antioxidant power and total phenolic contents are being recorded at 259.52 ㎕/㎎, 0.44±0.01, 15.29±0.04 ㎎ GAE/g respectively. The antioxidant activities of glutinous barley Jeung-pyun added with beet powder increase with increasing concentrations of beet powder. These results suggest that beet powder are useful as functional food resources within antioxidant activities.

This study was conducted to develop a Han-sik database as well as to assess the effects of Han-sik on dietary quality among Koreans. The Han-sik database was developed by a focus group composed of food and nutrition specialists considering the results of Han-sik perception surveys conducted in previous studies for frequently consumed dishes. Among the 1,322 dish items identified in the 4th (2007-2009) Korea National Health and Nutrition Examination Survey (KNHANES), 973 items (73.6%) were classified as Han-sik. Han-sik usage was defined as the percentage of Han-sik items of all individually consumed dish items in the dietary data of 22,113 subjects who participated in a 24-hour diet recall in the 4th (2007-2009) KNHANES. Dietary quality was evaluated based on adherence to the Korean Food Guidance System (KFGS), which was calculated as the percentage of the number of servings consumed in each food group out of recommended servings according to age and sex. Mean usage of Han-sik was 80.1% and was higher in older age groups, rural areas, as well as in households at the lowest income level. Han-sik usage was also higher on weekdays as well as for breakfast. Adherence to the KFGS was significantly higher for grains, meat · fish · egg · beans, vegetables, and fruits (p<0.001) but lower for milk · dairy products and oils · fats · sugars (p<0.001) across the quartiles of Han-sik usage. The results of this study indicate that Han-sik usage is high among Koreans. Further studies are needed to update the Han-sik database as well as investigate the association between health-related factors and Han-sik use.

The effects of incorporating yacon powder on the physicochemical properties and consumer acceptance of cookieswere investigated. With increased yacon powder content, the pH and density of cookies decreased significantly(p<0.05) while moisture content increased (p<0.05). Also the spread factor decreased with the addition of yaconpowder (p<0.05). Control showed the maximum value of hardness, but no significant differences were foundamong the 10-40% samples (p>0.05). Lightness and yellowness decreased significantly while redness increased sig-nificantly with a high content of yacon powder in the formulation (p<0.05). A consumer acceptance test indicatedthat the 10% sample received significantly higher scores in all attributes including overall acceptance (p<0.05).Cookies with 10% yacon powder are recommended to take advantage of the functional properties of yacon powderwithout sacrificing consumer acceptability.

The feasibility of incorporating jujube concentrate (JC) as a value-added food ingredient in convenience food prod-ucts was investigated using a model system of gelatin jelly. With an increasing level of JC added, the pH, moisturecontent, and lightness gradually decreased while the soluble solids content increased (p<0.05). Hardness and totalpolyphenol content also increased significantly with a higher amount of JC in the formulation (p<0.05). A con-sumer acceptance test indicated that the addition of JC had a favorable effect on consumer preferences in allattributes. Jelly with 20% JC is recommended (with respect to the overall preference score) for taking advantage ofthe functional properties of JC without sacrificing consumer acceptability.

주류를 제외한 과일 및 탄산음료 등을 제조하는 비알코올성 음료품 제조시설에서 발생되는 폐수는 높은 농도의유기물과 낮은 농도의 질소, 인 등을 함유한다. 이러한 폐수의 처리 시설은 주로 호기성 공정과 약품응집 공정으로 구성하고 후단에 사여과지 또는 활성탄 공정을 추가하기도 한다. 하지만 이러한 방식은 긴 체류시간과 침전지 설치로 인해 많은 부지를 필요로 하는 문제가 있다. 본 연구에서는 부지소요 문제와 슬러지 유출로 인한 수질저하 문제를 해결하고자 W식품공장 폐수처리장 인근에 MBR pilot plant를 설치하고 장기간 운영을 통해 데이터를 확보하고 처리 효율을 평가하였다. 약 3개월간음료수 제조공정 폐수를 평막을 적용한 MBR pilot plant로 운전조건을 변화하며 처리한 결과, 처리유량 20 m3/day, HRT 29hr, 4Q 반송조건까지는 유기물 제거율 97% 이상으로 안정적인 처리가 가능했다. 하지만 그 이상의 운전조건에서는 생물반응조의 오염물질 제거율이 감소하였고 TMP가 급격히 증가하는 모습을 보였다.

This study was to investigate the effects of differences in the temperature of steamed rice powder at the additionof wheat flour on the quality characteristics of Injeolmi after storage for 7 days at 4℃. The L values in color ofthe control and with 75℃ and 95℃ treatments decreased after storage whereas that of the 55℃ treatment increasedfrom 94.24 to 96.07. The amylose content of the control increased from 4.13% to 12.43%, while that of the 55℃,75℃, and 95℃ treatments increased from 5.10%, 5.28%, and 5.14% to 20.43%, 19.8%, and 19.34% respectively.Hardness of the 55℃, 75℃, and 95℃ treatments after storage increased by 109.2%, 2,023.2%, and 2,886.5%respectively compared with that at the initial storage stage. Hardness of the 55℃ treatment after storage was 3.17%compared with that of the control, which shows that the 55℃ treatment significantly reduced retrogradation inInjeolmi during storage. Total aerobic bacteria content of the control and the 55℃ treatment was 3.55 and 3.98 logCFU/g respectively, which were slightly higher numbers than those of the 75℃ (3.62log CFU/g) and 95℃ (3.30logCFU/g) treatments after storage for 7 days at 4℃.

This study aimed to evaluate the fermentation profile, organic acid composition, and volatile flavor components inthe meshes of Sul-dut prepared using different pre-treatment methods of rice (Godubap, Baeksulgi, and Ikbanjuk)and different fermentation agents (Nuruk and Ipguk). Among the three pre-treatment methods used, Ikbanjuk pro-duced the lowest alcohol content (12.2% for Nuruk and 12.6% for Ipguk) and the titratable acidity of the differentfermentation profiles seemed to be related to the degree of gelatinization during the pre-treatment of rice. Thedegree of gelatinizaion for Godubab and Baeksulgi was 100%, while that for Ikbanjuk was only 62.5%. Generally,titratable acidity was higher in the Ipguk group compared with the Nuruk group regardless of the pre-treatmentmethod. Citric acid was the major organic acid in the Ipguk group, while lactic acid was the major organic acid inthe Nuruk group. In particular, Ikbanjuk produced the largest amount of lactic acid in Nuruk. Volatile flavor com-ponents of Sul-dut were largely dependent on the fermentation agents and were less affected by the pre-treatmentmethod.

Grape seed tea (GST) was prepared by roasting grape seed at 150-190oC for 10-60 minutes and then soaking inwater at 100oC for 20 minutes. GSTs made with grape seed with an L value of 19.5-20.5 were the best in termsof color, flavor, and sensory test. GSTs prepared using roasted grape seeds had higher total phenolics and antioxi-dant activity than GST prepared from raw grape seed. Roasting times to reach an L value of 19.5-20.5 were over60 minutes at 160oC, 40-50 minutes at 170oC, 30-40 minutes at 180oC, and below 10 minutes at 190oC. In a sensorytest, the total preference increased depending on the degree of roasting. GSTs with the highest preference used grapeseed roasted at 170oC and 180oC for 30 minutes.