Two sorghum(Sorghum bicolor L. Moench cv. Hwanggeumchal-susu and Miryang 3) samples were milled using different milling methods, and their physicochemical properties were tested. Particle size was classified into five groups such as pin mill and low temperature-microparticulation(LTM; 10, 000, 20, 000, 30, 000, and 40, 000 rpm). The water absorption index (WAI), water solubility index(WSI), and a rapid Visco analyzer(RVA) were used to examine particle size distribution and color differences. Particle size of sorghum flour prepared using LTM was lower than that prepared using a pin mill. Particle size was further reduced by successive dry milling of the LTM flour. Lightness of colored pigments increased when particle size decreased. The WAI of Miryang 3 pin milling(M1) flour was the lowest after LTM, and WSI was higher in the order of M2, M3, M4, and M5. LTM sorghum flour had significantly higher pasting viscosity, as determined using a rapid Visco analyzer. LTM Miryang 3 sorghum flour(M2～M5) flour showed lower breakdown viscosity and higher final viscosity compared to those of M1 flour, resulting in an increased setback value.

The purpose of this study was to evaluate the appearance, sensory and physicochemical characteristics of muffin added glutinous(GSP) and non-glutinous sorghum powder(NGSP). As increasing the amounts of sorghum powder, proximate (moisture, ash and protein) and minerals composition(K, Ca, Mg and Na) of muffin were significant difference. The weight, volume, height, lightness(L-value) and yellowness(b-value) were decreased as increasing the amounts of sorghum powder. In the results of sensory evaluation, appearance, color, flavor, taste, texture and overall quality of untreated muffin were 1.4, 1.6, 1.4, 1.0, 1.1 and 0.9, and muffin added 10% GSP and NGSP were scored relatively high. Antioxidant compounds contents of muffin added sorghum powder were increased as increasing the amounts of sorghum powder. DPPH and ABTS free radical scavenging activities on methanolic extracts of untreated muffin were 0.74 and 1.31 ㎎ TE/g ER, respectively. DPPH and ABTS free radical scavenging activities of 100% GSP muffin were 9.40 and 19.14 ㎎ TE/g ER, and 100% NGSP muffin were 10.59 and 18.78 ㎎ TE/g ER, respectively. The results of this study show that notable antioxidant compounds and antioxidant activity on muffin added sorghum powder are considered to have significant health benefits.

The objectives of this study were to form comparisons of total polyphenol compounds, the antioxidant activities and the urushiol contents of lacquer tree(Rhus verniciflua) bark and the sensory properties of chicken soup was made with lacquer tree bark that was cultivated from different cultivation areas; Hamyang, Wonju and China. Total polyphenol contents of Hamyang, Wonju and China were estimated as 375.28±3.48, 403.60±6.6 and 311.06±4.99 ㎎/g. The total flavonoids contents of Hamyang, Wonju and China were measured as 374±14.12, 683.70±12.64 and 334.64±18.40 ㎎/g. The total phenolic compounds and flavonoids concentration, DPPH radical scavenging activity, and ABTS radical scavenging of lacquer tree cultivated in Wonju were higher than the others; Hamyang and China. The urushiol content of lacquer tree bark from Hamyang was 4.59±0.04 ppm and higher than others. Urushiol was not detected in China lacquer tree bark. Sensory evaluation tests for chicken soup containing lacquer tree bark showed that the scores of Wonju lacquer tree bark chicken soup was highest, however there are no differences between Hamyang, Wonju, and China significantly(p<0.05).

본 연구는 무용영재 판별 시, 보다 신뢰가 높고 타당하게 평가할 수 있는 평가방법은 무엇인 지 선행연구와 문헌을 통해 모색하는데 그 목적이 있다. 무용은 신체를 통해 표현하는 예술로서, 무용영재성을 판별하기 위해서는 신체골격이 완성되기 이전에 조기선발 되어야 한다. 하지만 조 기선발은 선발시기의 특성상, 무용영재로서의 잠재된 능력을 100% 판별해내기에는 다소 무리가 있으므로 무용영재성 판별은 매우 신중하게 고려되어야 한다. 일반 영재성 평가방법을 살펴보면, 학생의 영재성을 평가하는 교사평가방법과 자녀의 영재성 을 관찰하는 부모관찰방법, 그리고 자신을 평가하는 자기진단평가방법 등 다양하게 적용하고 있 다. 다양한 평가방법을 동원하여 영재성을 판별하는 이유는 단일한 평가방법에서 나타나지 않았 던 잠재된 영재성을 판별해 내고, 판별결과에 대한 오류를 최소화하기 위해서이다. 이에 따라 일 반 영재성 평가도구는 평가자의 특성을 고려하여 다양하게 개발되고 있으며, 예술분야에서도 예 술영재성 평가도구 개발에 관심을 기울이고 있다. 특히 무용영재성 판별에 관한 선행연구 중에서 도 무용영재성을 객관적으로 평가할 수 있는 측정도구를 개발(이진효, 2010)하여, 보다 신뢰가 높 고 타당하게 무용영재를 선발할 수 있는 기틀을 마련하였다. 하지만 앞서 언급하였듯이, 무용영 재는 조기선발이라는 선발시기를 고려해볼 때, 잠재되어 있는 능력을 다차원적으로 평가하기 위 해서는 선행연구에서 개발된 교사평가도구만으로는 부족하다. 따라서 본 연구는 일반영재성 판별 방법을 통해 무용영재성 판별의 타당성을 높이는 평가 방법을 모색하고, 국내·외 영재기관에서 는 어떤 평가도구를 통해 무용영재를 선발하는지 분석하였다. 본 연구를 통해 분석된 무용영재성 판별 방법은 추후 무용영재성 평가도구를 개발하고자 할 때, 유용한 자료로서 제공될 뿐만 아니라 무용영재로서 선발된 이후, 후천적인 노력을 통해 발현 되는 무용영재성 요인에 대해서도 보다 객관적으로 평가할 수 있는 평가기준을 제공할 것이다.

The purpose of this study was to assess the influence of patellar height on quadriceps muscles' electromyography (EMG) activity during a squat exercise in adults with patella baja. For the study, we recruited 15 volunteers who had patella baja on the right side. We measured the EMG activity of the right rectus femoris, vastus medialis oblique, and vastus lateralis muscles during squat exercises under two conditions, specifically with and without an infra-patellar strap. The infra-patellar strap was applied below the tested patella to elevate the patella to a normal height. A paired t-test was used to compare the effects of patella height on EMG activity of the quadriceps muscles. The EMG activity of the rectus femoris (RF), vastus medialis oblique (VMO), and vastus lateralis (VL) muscles were significantly decreased during the squat exercise with the infra-patellar strap compared to the same exercise without the infra-patellar strap (p<.05), while the VMO/VL ratio was not different significantly between two conditions (p>.05). The findings of this study suggest that an infra-patellar strap may benefit people with patellar baja, as changes in patellar height could improve the efficiency of the quadriceps muscles.

Recurrence-metastasis status of squamous cell carcinoma of tongue is a challenging oncologic problem. This study examined the expression of E-cadherin/β-catenin cell adhesion complex in squamous cell carcinoma of the tongue through an immunohistochemical study. Twenty samples from 15 patients with squamous cell carcinoma of the tongue, who were treated at the Department of Oral and Maxillofacial Surgery, consisted of primary or recurrent tumors along with matched metastatic lymph nodes were retrieved for immunohistochemical staining and grouped based on recurrence-metastasis status.Differences in stain localization were noted in E-cadherin, β–catenin and phospho β–catenin staining between different tumor groups based on the recurrence-metastasis status. The number of phospho β-catenin stain positive cells was found to have a significant role in survival. E-cadherin confirms its role as a powerful individual differentiation indicator and the role of β-catenin specially the phospho type elicts interest

국예비 해기사 교육의 일환으로 심화교육의 일환으로 IMO 활동, 국제적인 주요협약 및 규정의 종류, 체계 및 전반적인 내용이해를 교육하기 위한 산학간 해기사 교육프로그램을 제안한다. 전체적 교육의 구성, 교육결과에 대한 만족도 분석을 통하여 바람직한 교육프로그램을 제시한다. 교육의 결과로서 미래 해기사의 IMO 활동에 대한 관심을 드높이고, 참신한 아이디어 발굴을 통한 국가 의제개발의 씨앗을 창출해내 는 역할이 기대되고, 해기사의 자긍심을 고양시키는 부수적인 잇점도 가질 수 있을 것이다.

The effects of an abdominal drawing-in maneuver (ADIM) using a pressure bio-feedback unit (PBFU) were compared to the effects of a pelvic belt (PB) on the muscle activities of the hip and back extensor muscles during hip extension in the prone position. Fifteen healthy male participants all performed prone hip extensions under three conditions: 1) preferred hip extension (PHE), 2) performing an ADIM, and 3) using a PB. The muscle activities of the erector spinae, the gluteus maximus, and the medial hamstring on the right side were recorded by surface electromyography. The muscle activity of the erector spinae was significantly lower while performing an ADIM during prone hip extension than during PHE or with a PB (p<.05). Gluteus maximus muscle activity was significantly higher while performing an ADIM (p<.05). No significant difference was found for the medial hamstring muscle among the three conditions (p>.05). We concluded that the internal stabilization of the pelvis and lumbar spine afforded by the ADIM using a PBFU could be more effective than the external stabilization provided by a PB in terms of increasing selectively gluteus maximus activation during prone hip extension.

This study was conducted to investigate the residue amount of harmful materials on the 113 commercial fruit teas (Gugija, Omija, Sansuyu) in Gwangju area. It was performed using the GC-ECD, GC-NPD, GCMSD and the LC-UVD, LC-FLD, LC-MSD to analyze 200 pesticides. The heavy metals were determined using a Mercury analyzer and AAS. The sulfur dioxides were analyzed by modified Monnier-Williams method. The residual pesticides were detected in 4 samples (Gugija). The mean values of heavy metal contents (mg/kg) were Pb, 0.024; Cd,0.031; As, 0.010; Hg, 0.003. The measured values of Pb, Cd, As, Hg showed within MRLs. The sulfur dioxides were over MRLs in 4 samples (Gugija). These results will be used to establish on the regulation of commercial fruit teas in Gwangju area.

A blood test is a laboratory analysis performed on a blood sample that is usually extracted from a vein in the arm using a needle, or via fingerprick. They are used to determine physiological and biochemical states, such as disease, mineral contents, drug effectiveness, and organ function. Although the term blood test is used, most routine tests (except for most haematology) are done on plasma or serum, instead of blood cells. Main advantage of using saliva in diagnostics is easy and non invasive sample taking compared to peripheral blood. According to the study published, saliva contains more than 20 percent of the proteins found in blood. The purpose of present study is to compare biochemical enzymes in saliva and in blood serum and to evaluate the usefulness of saliva specimens instead of blood in dental clinic. The saliva from 215 healthy over 50 years of aged people lived in Dong-gu district, Gwangu city was collected and the analysis was performed by six enzyme-linked immunosorbent assays (ELISA). ELISA results were compared with blood chemistry results. The values or patterns on Alanine Aminotransperase (ALT), Aspartate Aminotransperase (AST), Cholesterol and Triglyceride in saliva were not correlated with those in blood serum. However, Albumine and γ-glutamyl transpeptidase (γ-GTP) were followed the positive relationship with blood chemistry. These result showed that detection and identification of Albumine and γ-GTP level could be established by saliva ELISA analysis, so that ELISA assay on saliva could be useful alternative to serum testing.

One-step dilution and direct transfer would be a practical technique for the field application of frozen embryo. This study was to examine whether Jeju Black Cattle (JBC, Korean Cattle) can be successfully cloned from vitrified and one-tep diluted somatic cell nuclear transfer (SCNT) blastocyst after direct transfer. For vitrification, JBC-SCNT blastocysts were serially exposed in glycerol (G) and ethylene glycol (EG) mixtures〔10% (v/v) G for 5 min., 10% G plus 20% EG (v/v) for 5 min., and 25% G plus 25% EG (v/v) for 30 sec.〕which is diluted in 10% FBS added D-PBS. And then SCNT blastocysts were loaded in 0.25 ml mini straw, placed in cold nitrogen vapor for 3 min. and then plunged into LN2. One-step dilution in straw was done in 25℃ water for 1 min, by placing vertically in the state of plugged- end up and down for 0.5 min, respectively. When in vitro developmental capacity of vitrified SCNT blastocyst was examined at 48 h after one-step dilution, hatched rate (56.4%) was slightly lower than that of control group (62.5%). In field trial, when the vitrified-thawed SCNT blastocysts were transferred into uterus of synchronized 5 recipients, a cloned female JBC was delivered by natural birth on day 299 and healthy at present. In addition, when the short tandem repeat marker analysis of the cloned JBC was evaluated, microsatellite loci of 11 numbers was perfectly matched genotype with donor cell (BK94-14). This study suggested that our developed vitrification and one-step dilution technique can be applied effectively on field trial for cloned animal production, which is even no longer in existence.

The purpose of this study was to assess visual biofeedback's influence on trunk muscles' (EMG) activity and endurance holding time for correct position during whole-body tilt exercise. For the study, we recruited 14 volunteers who showed no symptom of lumbar disease during medical tests. We measured the EMG activity of their rectus abdominis, external abdominal oblique, internal abdominal oblique and erector spinae muscles, and their endurance holding time for correct position during anterior and posterior whole-body tilt under two conditions: whole-body tilt with and without visual biofeedback. Resistance with gravitational force on the trunk during whole-body tilt was applied by using a device that had a monitor on which the subjects could check their alignment and that sounded an alarm if a subject's alignment collapsed. The study showed an increase in the EMG activity of external abdominal oblique, internal abdominal oblique/rectus abdominis ratio and endurance holding time for correct position during both anterior and posterior whole-body tilt with visual biofeedback compared with without visual biofeedback (p<.05). We suggest that the whole-body tilt exercise with visual biofeedback could be a beneficial strategy for selectively strengthening the internal abdominal oblique muscle and minimizing the rectus abdominis muscle's activity while maintaining correct alignment during whole-body tilt exercise.

This research was carried out as a survey on the contents of Lead (Pb) and Cadmium (Cd) in 5 type agricultural products unestablished safety guideline in Korea. The average levels of Pb: onion 0.010 mg/kg, cucumber 0.018 mg/kg, garlic 0.012 mg/kg, green pepper 0.027 mg/kg, sesame 0.029 mg/kg. The average levels of Cd: onion 0.006 mg/kg, cucumber 0.002 mg/kg, garlic 0.008 mg/kg, green pepper 0.011 mg/kg, sesame 0.024 mg/kg. The present result of this study showed that Pb and Cd contents in the whole samples were less than the maximum residual levels of the codex standard. The levels of exposure assessment for Pb and Cd by intake from vegetables and sesame were merely at 1.3 × 10−⁴(mean) ~5.1 × 10−⁴(95th percentiles) μg/kg bw/day for Pb, 7.5 × 10−5(mean)~3.6 × 10−⁴(95th percentiles) μg/kg bw/day for Cd. The data from this research will be valuable source for database construction for science-based safety control and management for the trace metal contamination in food including agricultural products.

목적 : 본 연구의 목적은 스노즐렌 치료 적용이 발달장애 아동의 문제행동 변화에 미치는 영향을 알아보고, 이들 결과를 신경 생리적으로 검증하기 위해 뇌파의 변화를 알아보고자 하였다. 연구방법 : 연구의 대상은 2명의 발달장애 아동이었고, 연구 설계에는 개별사례 실험 연구 중 ABA 설계를 사용하였다. 실험의 절차는 기초선(A1)에서의 2회기, 치료과정(B)에서의 8회기, 치료추적(A2)조사에서의 2회기로 모두 12회기로 이루어졌다. 치료는 스노즐렌 치료실에서 30분 동안 비지시적인 아동중심치료접근법으로 각 아동에게 제공했다. 종속변수인 문제행동검사(CBCL)는 치료전과 후에 각 1씩 실시하였고, 뇌파 측정은 연구의 전 과정동안 매 회기마다 이루어졌다. 뇌파 자료는 파워 스펙트럼 분석을 통해 정량적으로 분석하였는데, 결과의 분석을 위해 상대뇌파(쎄타파, 알파파, 베타파, 감마파) 값을 선택하였다. 결과 : 연구 결과, 문제행동검사의 전체 점수는 스노즐렌 치료 후 두 아동 모두 감소함을 보여 스노즐렌 치료의 긍정적인 행동변화 효과를 알 수 있었다. 뇌파 변화의 결과에서는 상대뇌파 분석에서 대상자들 모두가 쎄타파의 감소와 알파파의 증가를 보였고, 대상자2는 이와 함께 감마파의 큰 감소를 보였다. 결론 : 본 연구를 통하여 스노즐렌 치료 적용이 발달장애 아동의 문제행동과 뇌파 변화에 영향을 미친다는 것을 알 수 있었다. 본 연구는 발달장애 아동의 문제행동 완화와 이에 관련된 뇌파 변화를 검증함으로써 스노즐렌 치료의 임상적 유용성을 확인하였다.

본 논문은 합성형 성장호르몬으로 천연 프로게스테론에 비해 20~30배 강하게 작용하는 초산메드록시프로게스테론 에 대한 정량한계, 표준물질의 직선성, 회수율 등의 유효성 평가 및 내부표준물질 선정을 통한 분석방법 최적화를 식품공전의 시험법을 기초로 수행하고, 이를 바탕으로 국내 유통식육 내 초산메드록시프로게스테론의 잔류량을 조사 하였다. 그 결과 초산메드록시프로게스테론의 검출한계는 1.5 ug/kg, 정량한계는 5.0 ug/kg 으로 나타났으며 표준물질의 좋은 직선성 (r2= 0.9968)을 확인 하였다. 내부표준물질 의 비교결과 식품에 오염될 우려가 없는 progesterone-D9으 로 선정하였다. 이를 시험한 결과 7.69분의 머무름 시간을 가지며, 정량이온은 m/z 324-100, 정성이온은 m/z 324-113 으로 설정하였다. 내부표준물질에 대한 정량의 회수율 실험결과 67.5~109.5%로 높아진 회수율을 나타낸 것을 알 수 있었다. 이와 함께 전국의 서울특별시 및 6개 광역시에서 수거한 국내산 쇠고기 46건, 수입산 쇠고기 43건, 국내산 돼지고 기 60건, 수입산 돼지고기 12건과 국내산 닭고기 35건의 초산메드록시프로게스테론의 잔류량을 실태 조사한 결과 검출된 경우는 한건도 없었다. 따라서, 초산메드록시프로게 스테론에 대한 유통 식육은 안전한 것으로 조사되었다.

This study was to investigate the effect of flavonoid treatment on in vitro development of bovine somatic cell nuclear transfer (SCNT) embryos, and their pregnancy and delivery rate after embryo transfer into recipient. In experiment 1, to optimize the flavonoid concentration, parthenogenetic day 2 (≥ 2-cell) embryos were cultured in 0 (control), 1, 10 and 20 μM flavonoid for 6 days. In the results, in vitro development rate was the highest in 10 μM flavonoid group (57.1%) among treatment groups (control, 49.5%; 1 μM, 54.2%; 20 μM, 37.5%), and numbers of total and ICM cells were significantly (p<0.05) higher in 10 μM flavonoid group than other groups. We found that 10 μM flavonoid treatment can significantly (p<0.05) decrease the apoptotic index and derive high expression of anti-oxidant, anti-apoptotic, cell growth and development marker genes such as Mn-SOD, Survivin, Bax inhibitor, Glut-5, In-tau, compared to control group. In experiment 2, to produce the cloned Jeju Black Cattle, beef quality index grade 1 bull somatic cells were transferred into enucleated bovine MII oocytes and reconstructed embryos were cultured in 10 μM flavonoid added medium. When the in vitro produced day 7 or 8 SCNT blastocysts were transferred into a number of recipients, 10 μM flavonoid treatment group presented higher pregnancy rate (10.2%, 6/59) than control group (5.9%, 2/34). Total three cloned Jeju Black calves were born. Also, two cloned calves in 10 μM flavonoid group were born and both were all healthy at present, while the one cloned calf born in control group was dead one month after birth. In addition, when the result of short tandem repeat marker analysis of each cloned calf was investigated, microsatellite loci of 11 numbers matched genotype between donor cell and cloned calf tissue. These results demonstrated that the flavonoid addition in culture medium may have beneficial effects on in vitro and in vivo developmental capacity of SCNT embryos and pregnancy rate.

The purpose of this study was to investigate the variations in gait parameters in terms of the type of arm sling used in hemiplegic patients. Ten patients with hemiplegia and ten healthy adults participated in this study and walked at self-selected speeds on a GAITRite-instrumented carpet. The activities of the opposite shoulder girdle muscle including the latissimus dorsi, anterior deltoid, and posterior deltoid were simultaneously recorded using surface EMG during gait. They were randomly assigned a condition: without an arm sling, a single strap arm sling, a Harris hemi arm sling, a Rolyan humeral cuff arm sling, and a Bobath roll arm sling. The following gait variables were analyzed: the temporo-spatial parameters of velocity, step length, stride length, swing phase, stance phase, single support, step time and toe in/toe out. The statistical analysis was one-way ANOVA with repeated measures to compare the variation of each variable. In comparison of parameters in each trial in the hemiplegia group, the non-affected side stride length, single support, and toe in/toe out resulted in statistically significantly changes (p<.05). But without an arm sling group did not show any gait parameter differences with arm slings. This study found that several arm slings varied gait patterns in patients with hemiplegia and in healthy adults. In the EMG analysis, the Rolyan humeral cuff arm sling and the Bobath roll arm sling were higher muscle activity for the latissimus dorsi muscle than did the single strap ann sling. Further study should examine the problems that appeared in patients who worn arm slings by focusing on a larger number of subjects and by studying the variety of responses in more detail using an assessment tool that measures variation.