The purposes of this study are to evaluate the development of disabled children us- ing LAP(Learning Accomplishment Profile) and to investigate the correlation between subtest scores of LAP. The development scores were obtained using 244 items in 5 su-btests (gross motor, fine motor, cognitive, language/cognitive, and self help) of LAP in 34 children with delayed development from February, 1998 to March, 1999. The birth history and risk factors for developmental disablilty such as prematurity, hyperbilirubi-nemia, low birth weight, and seizure were evaluated. Collected data were analized by Pearson's correla tion coefficient and Mann-Whitney U test. The results were as follow : 1) Among the subtests of LAP scores, fine motor, language/cognitive, and self-help were signi ficantly correlated with the cognitive subtests scores (p > 0.001) 2) On the other hand, gross motor subtest scores were independent to cognitive sub-test scores. 3) The children who had history of seizure during neonatal period showed significantly low scores in language-naming, fine motor-writing, and self help-eating. The LAP is considered as a useful tool to evaluate the cognitive development disab- led children. Cognitive function significantly influence on other developmental areas es-pecially on fine motor development.

The purpose of this case study was to introduce botulinum toxin A injection in cerebral palsy. Spasticity can be managed using a variety of methods. Eliminating aggravating sources, promoting stretching and bracing, and positioning are the least invasive methods of treatment. Botulinum toxin A injection is a relatively recent method of spasticity management in children with cerebral palsy. A 3-year old boy was evaluated for possible botulinum toxin injection to promote left side function. The patient had left hemiparetic cerebral palsy. He walked with bilateral intoning, much worse on the left than on the right and with excessive plantar flexion on the left. Botulinum toxin A was injected into the left medial gastrocnemius, with the goals of improving quality of gait. Finally, botulinum toxin treatment of would improve the motor function and ambulatory status in cerebral palsy by hypertonicity, spasticity, dynamic contracture and athetoid movement.

은 튜브에 장입되기 전의 초기 분말의 입자 크기가 Ag/Bi-2223초전도 선재의 미세구조와 상전이, 임계 전류 밀도등에 미치는 영향에 대해 고찰하였다. 분말의 입자 크기는 하소 분말을 볼밀을 이용하여 0-48 시간 동안 분쇄하여 조절하였다. 열처리 후 최종 초전도 선재의 전기적 성질은 초기 분말의 입자 크기에 영향을 받는 것으로 나타났으며 분말의 분쇄에 의한 반응성의 증가에 의해 열처리시 2223 상으로의 상전이가 빠르게 일어났고 이차상의 크기와 분율이 감소된 미세구조를 얻을 수 있었다. 그러나 과다한 분쇄에 의한 반응성의 증가에 의해 열처리시 2223상으로의 상전이가 빠르게 일어났고 이차상의 크기와 분율이 감소된 미세구조를 얻을 수 있었다. 그러나 과다한 분쇄는 분말의 비정질화를 유발하여 2223 상으로의 전이를 방해함으로써 선재의 임계 전류 밀도를 감소시키는 결과를 나타내었다.

The purpose of this study is to explore the daily meal pattern in Seoul families. The informations of this study were obtained from 443 housewives in Seoul during August 20 to September 20, 1994, by using questionaires. The χ^2 test was applied to see the individual data. The following observations were made in this study. 57% of Seoul families had rice with side dish set three times a day, the first choice for rice substitute was bread in breakfast and noodle in lunch, 98% of the subjects had rice in dinner. The change of the meal pattern from boiled rice to bread and noodle was the result of social changes such as urbanization, heightening up of education level of woman and the resulting increase of woman employment. They were consumed 2∼5 varieties of side dishes. The favorite were Kimchi and Cigae` and Guk. The major meal pattern is Seoul is still the traditional one composed of boiled rice and side dishes.

본 연구(硏究)는 식물(植物)의 발생(發生)과 분화(分化)에 관(關)한 생화학적(生化學的) 분자생물학적(分子生物學的) 구명(究明)을 위해 조직(組織) 발생(發生) 단계(段階)의 특이적인 유전자(遺傳子)의 발현조절 기작과 역할(役割)을 연구(硏究)하기 위한 기초(基礎) 지식을 얻고자 수행(遂行)되었다. 벼를 실험재료(實險材料)로 하여 현미로부터 callus를 유도(誘道)하고 이들 callus에서 여러가지 방법(方法)을 통(通)해 embryogenic callus와 nonembryogenic callus를 분리(分離)해 somatic embryogenesis에 관련된 특수한 단백질(蛋白質)을 찾고자 하였고 그 결과(結果)를 요약(要約)하면 다음과 같다. 지금까지의 보고(報告)에 의하면 재분화가 잘되는 callus와 그렇지 않은 callus간에는 여러가지 면에서 차이가 난다고 알려져 있다. 재분화가 잘되는 callus는 표면이 거칠고 흰색에 가까우며 조직(組織)이 단단하다. 반면에 재분화가 되지않는 callus는 표면(表面)에 윤기가 나며 단단하지 않다. 이러한 보고(報告)를 기초(基礎)로 하여 media에 1mg/1의 2,4-D가 첨가(添加)된 배지(培地)에서 2주 정도 callus를 유기시키고 유기된 callus는 다시 배양(培養)하였다. 7-8차 계대배양하면서 embryogenic callus와 nonembryogenic callus를 분리(分離)한 다음 이를 시료(試料)로 하여 protein을 추출(抽出)하여 SDS-PAGE 상에서 비교하여 보았다. 그 결과(結果) 두 callus간에 차이가 있음을 알 수 있었다. 7-8차 계대배양한 두 종류(種類)의 callus로 부터 protein을 분리하여 two-dimensional gel 전기영동에 의해 비교하여 본 결과(結果) 몇 개의 단백질(蛋白質)이 서로 다름을 알 수 있었다.

In the view of the fact that data are scare concerning the change of Methionine composition of poultry meat in function of storage temperature and cooking method. The present study was carried out to change in these compounds and the results were summarized as follows. 1. The result of measuring methionine compound of raw material stored under the condition of 5℃, 10℃ for 6 weeks were increased as temperature increases. 2. The result of change in methionine compound of roasting were decreased than raw material It was greatly decreased in the storage for 42 days at 10℃ and slightly decreased in the storage for 14 days at 5℃ 3. When the boiling in the water, methionine compound was decreased than roasting to compare with raw material 4. By the results of this study, one of the best cooking method is roasting, because of the change in methionine compound of roasting is slightly decrease.

목적: 본 연구의 목적은 대학 여자축구선수들이 재활과정에서 느끼는 심리적 변화를 탐색하고 이를 극복할 수 있 는 전략을 살펴보는 데 있다. 방법: 참가자는 2018년 대학 여자축구연맹에 등록되어 있고, 부상으로 인한 재활경 험이 있는 7명의 선수이다. 이들을 대상으로 개인 면담과 집단면담을 시행하여 수집된 자료를 구성주의 근거이론 (Constructing Grounded Theory) 접근방법을 통해 분석하였다. 결과: 선수들은 재활 과정 초기에서 부정적인 생각 을 하게 되는 동시에 긍정적으로 생각하려는 노력이 순환적으로 반복되는 심리적인 양상을 겪으며, 선수 생활에 대한 양가감정을 느끼는 것으로 나타났다. 재활 과정 중기의 심리적 변화에서는 재활상황 자체에 대한 초조함과 불안함, 심리적 압박감을 느끼면서도 점차 회복하는 신체에 자신감을 얻고 복귀를 위한 노력에 집중하는 것으로 나타났다. 재활 과정 후기에서는 복귀에 대한 설렘을 가지면서도 재발 불안감 등으로 부담과 압박을 느끼는 복귀 에 대한 양가감정이 드러났다. 선수들의 경험을 바탕으로 구성한 극복전략은 다음과 같다. 첫째, 자신감 향상전 략, 긍정적 사고의 전환 등의 심리적 극복, 둘째, 복귀를 위한 훈련을 포함한 신체적 극복, 셋째, 여자선수들을 위 한 전문적 프로그램 필요의 환경적 지원, 넷째, 주요타자지원과 같은 사회적 지원이다. 결론: 대학 여자축구선수 들은 재활과정에서 여러 가지 복합적인 심리적 변화를 겪으며, 선수로서의 삶을 진지하게 고민해보는 양가감정의 갈등이 함께 나타나는 것을 알 수 있었다. 이렇게 드러난 양가감정의 폭을 줄이거나 해결하는 심리적 과정은 선 수로서의 정체성을 재확립하는 계기가 될 수 있다. 이를 위해서는 이에 기반하는 내용을 포함한 전문적인 스포츠 심리상담과 체계적인 지원이 재활과정의 선수들에게 필요할 것이다.

본 연구는 지진계측시스템이 설치되지 않은 중소형 교량의 지진손상 수준을 평가하기 위하여 대상 중소형 교량 인근에 위치한 지진관측소의 지진관측 데이터를 이용하여 대상 교량위치에서의 지반응답스펙트럼을 추정하기 위한 알고리즘을 제시하였다. 일반적으로 중소형 교량의 내진설계 및 성능평가는 동적해석법 중 응답스펙트럼해석법이 가장 널리 활용되고 있으므로 대상 중소형 교량에 대한 평가 지진력으로 지반응답스펙트럼을 적용할 수 있는 알고리즘을 제시하였으며, 제안된 알고리즘을 이용한 프로그램 알고리즘도 제안하고 제안된 알고리즘을 통하여 실제 지진계측데이터를 이용하여 특정 위치에서의 지반응답스펙트럼 추정 예를 나타내었다.

BAT-AEL(Best Available Techniques Associate Emission Level) is the basis for establishing permissible emission standards for the workplace. Therefore, it is necessary to formulate a regulated BAT-AEL setting methodology that is generally applicable to all relevant industries. For the BAT-AEL settings, various factors should be considered such as the pollutants item, whether the workplace is subject to integrated pollution prevention and control, whether BAT is applicable, the basic data type, the emission classification system, and the suitability of the collected data. Among these factors, it is the most important factor to establish the classification system for the emitting facilities such that the emission characteristics of an industrial facility and its pollutants can be effectively reflected. Furthermore the target of the survey workplace should adhere to the BAT guidelines, even if it is a workplace that is subject to an the integrated environmental system. Certified data (SEMS, TMS, cleanSYS, WEMS, etc.) can be used to prioritize the classification system for the emission facility and the emission levels of pollutants. However, the self-measured data, daily logs, and questionnaire data from the workplace can also be used upon agreement of the relevant TWG. The collected data should only be used only when the facility is operating normally. Data that have been determined to be outliers or inappropriate validation methods should also be excluded. The BAT-AEL can be establish by adhering to the following procedure: 1) investigate all relevant workplaces with in the industry, 2)select workplaces for integrated management, 3)Identify BAT application, 4)identify whether BAT is generally applicable, 5)establish a classification system for emitting facilities, 6)collection available data, 7)verify conformity, 8)remove of outliers, 9)prepare the BAT-AEL draft, 10)deliberate, and 11) perform the confirmation procedure.

Background: Various Schisandra chinensis (SC) varieties grow in diverse regions in Korea. However, there is no valid scientific evidence of these varieties. This study aimed to select the excellent resources in terms of the growth characteristics, antioxidant activities, and analysis of the active compounds of the SC collection. Method and Results: In total, 154 resources of SC were collected from various regions of Korea. The growth characteristics were measured by the number of fruit bunches, fruit number, and weight of 100 fruits. The antioxidant activities were investigated by analyzing the total flavonoid and total polyphenol contents and the radical scavenging activity of DPPH and ABTS. Schizandrin A, schizandrin c, gomisin a, and gomisin N were analyzed by HPLC. Each resource showed different growth characteristics. Among the antioxidative effects, the highest 20 resources showed high antioxidant activities in selected 29 resources. Analysis of the SC lignan index showed that all resources contained more than 1.16% of active compounds. Conclusions: All of the selected 29 SC resources were shown to have excellent growth characteristics, antioxidant activities, and bioactive compound richness. Especially, SC-004, SC-007, and SC-154 showed the best growth characteristics, and SC-22, SC-40, and SC-45 showed the best antioxidant activities and bioactive compound richness.

This study was carried out to investigate the economic value of organic rice production using gelatin ․ chitin microorganisms in eco-friendly complex, Gongsan, Naju city. The soil condition of experiment paddy field was Jeonbuk series and silt loam with a slightly poor drainage. Except for the high effective silicate, the chemical characteristics of soils used were included in the optimum range of paddy soils in Korea. In growth, plant length, tiller number, ear number, and ear length were observed to be higher in conventional paddy fields than organic paddy fields. However, number of grain per panicle and grain filling ratio (%) were higher in organic paddy fields than conventional paddy fields. Incidences of diseases and insect pests were slightly higher in the organic paddy fields. Water weevil, sheath blight, rice leaf roller and rice blast were more occurred in organic paddy field. On the other hand, false smut was higher occurred in conventional paddy field. There was a significant negative correlation between rice sheath blight and rice leaf roller, and rice yield. In the milled rice quality, the quality of organically cultivated milled rices was lower by the increase of broken rice than that of conventionally cultivated milled rices. The quality and palatability of rice were higher in organic cultivation with decreasing of protein content. Net income of conventionally and organically cultivated rice was 360,000 won/10a and 610,000 won/10a, respectively. Premium net income of the organically cultivated rice was 68%.

Background: For stable induction of tetraploidy in Fallopia multiflora Haraldson, colchicine was treated to establish the condition of induction and investigated the morphological and cytogenetic traits of the tetraploid plants obtained compared to those of diploid ones. Methods and Results: For the induction of tetraploidy, F. multiflora plants were soaked in aqueous solutions of colchicine at various concentration (0.1, 0.5, and 1.0%). After this, 2% dimethyl sulfoxide (DMSO) was added at room temperature on a shaker set at 150 rpm for periods of 12, 24, and 48 h. The induction rate of tetraploids appeared to be the highest in plants treated with 0.5% colchicine for 24 h. As the colchicine concentration and soaking time increased above these levels, the growing tip of the roots did not develop and they began to rot. When compared to diploid plants, tetraploids differed greatly in various characteristics, including the sizes and shapes of the leaves, fruits, flowers and roots. The induced tetraploid F. multiflora had larger guard cells, and chloroplasts, increased number of chloroplast in the guard cells and decreased stomatal densities. Conclusions: When colchicine induced plants for tetraploid, it can be distinguished from diploids, in various characteristics such as morphological changes as stomatal size, number of chloroplasts per guard cell, number of chromosomes and flow cytometry. Therefore, it proved that these methods are suitable, quick and easy methods for the identification of the ploidy level of F. multiflora.

Background : Rehmannia glutinosa root has been commonly used as traditional medicine in Korea. This root including prepared rehmannia that is the steamed root of rehmannia can be also used as food. This study was performed to analyze the quality properties of the root and the tea with different steaming conditions to evaluate the potential as a tea. Methods and Results : For this study, we used ‘Dagang’, new variety of R. glutinosa, developed by NIHHS. The root was dried and steamed for 9 times. Every steaming, powder and extract were used for pH, antioxidant activity analysis (total phenolic acid and total flavonoid content) and sensory evaluation. As steaming repeated, pH was decreased. The total phenolic acid and total flavonoid content was increased during steaming process. In the sensory evaluation for the tea, 56% of respondents (A group) recognized the difference between 6th and 9th steaming tea correctly but 44% (B group) didn’t. The sensory scores showed that 6th and 7th steaming tea had higher overall preference scores than other samples. This tendency was more clear in the A group. Depending on the age group, there were significant different tendency among 3 age groups (20 – 30 s, 40 – 50 s, over 60 s). The youngest group was preferred 6th than 9th steaming tea. On the other hands, 9th steaming tea had more priority than 6th steaming tea in older age group. Conclusion : This study showed a potential of R. root as a tea material. The quality properties of the R. root are affected by steaming conditions and the preference tendency of tea was different depending on the age group. These data can be used to develop tea material suitable for consumer age and establish optimal steaming conditions in industrial use.

Background : The causative organism of Astragalus membranaceus (Hwanggi) root rot disease was known as Pythophthora drechsleri. In this study, a new species of root rot causative organism has been identified and reported. Methods and Results : Hwanggi, which has root rot disease, was collected in Jungsun-gun, Gangwon-do. The diseased root was cut into 1 ㎝ size and disinfected with 1% sodium hypochlorite solution for 1 minute and washed with sterile distilled water. The dried samples were dipped in the PDA medium and cultured in at 25℃ incubator. The 21 isolates were cultured under the same conditions and genomic DNA was extracted. The genomic DNA of isolated strains was extracted using a kit of Biofact. The isolated strains were identified using internal transcribed spacer (ITS) region sequencing. The ITS region was amplied using the primer pair ITS1 (5’-TCCGTAGGTGAACCTGCGG-3’) and ITS4 (5’-TCCTCCGCTTATTGATATGC-3’). The PCR was performed in a final volume of 50 ㎕ containing 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5 mM MgCl2, each dNTP at a concentration of 0.2 mM, 1.25 IU of Taq polymerase, each primer at a concentration of 10 pmol, and 2ul of the DNA template. Sequencing was performed by asking Macrogen. The analyzed sequences were compared using the Blast program provided by NCBI. As a result of identification of 21 strains, 13 Fusarium spp., 1 Peronospora sp. (causative organism of downy mildew), 3 Acremonium spp., 3 Uncultured fungus, 1 Phyrtophthora sp.. The main pathogen of wilt disease was known as Fusarium sp., a soil-borne pathogen. After analyzing the systematic taxonomic relationships, the systematic diagram was created using the neighbor-joining method. Conclusion : We have finally identified Phytophthora cryptogea as a causative organism of Hwanggi root rot disease and will check whether the disease has occurred on the inoculation through pot test.

Background : Rehmannia glutinosa (RG) has been utilized as a traditional medicine in Asia. However, the development of varieties is limited and the climate is changing gradually. Therefore, it is required to develop a superior lineage suitable for this. So we have secured several species, and it is necessary to confirm the cytotoxicity of various kinds of cells for its safety and to secure safety for further utilization. Methods and Results : 11 cultivars and 21 lineages of RG were collected from Rural Development Administration (RDA) at Eumseong of Chungcheongbuk-do and national farmhouse. They were cultivated in test-research farm in RDA and used as materials. Human (THP-1 cell, human leukemia monocytic cell line) and rodent-derived immune cells (RAW264.7, murine monocyte/macrophage cell line) and hepatocytes (HepG2, human liver cell line) were used to assess cytotoxicity. Cytotoxicity was determined by using MTT assay. As a result of evaluation of cytoxicity, 11 cultivars and 21 lineages of RG were not shown cytotoxicity range from 250 - 1,000 ㎍/㎖ concentration in THP-1 cell, RAW264.7 cell and HepG2 cell. Conclusion : Development of RG with superior lineage suitable for changing climate is required. We selected a good lineage (21 ea), and result of the cytotoxicity evaluation from low to high concentrations in immune- and liver-derived cells, there was no toxicity at all. Therefore, if these excellent lineages are distributed to farmers, they can help farmers. And it can help research on immunity and liver function in the future.

Background : This study was carried out to introduce Atractylodes macrocephala as a new income element in Gangwon area and to develop the technology necessary for stable quality seedling production. Methods and Results : For the production of high quality seedlings of Atractylodes macrocephala, seedling growth characteristics were investigated according to the types of plug cell size and seedling raising period. Atractylodes macrocephala seeds were sown on February 14, 2018 in 72, 105, 128, 162, and 200 plug trays. The emergence period after sowing was March 2, and the final occurrence rate was 76.6 - 79.5%. The number of days of emergence took 18 to 20 days from sowing date. Growth of seedlings tended to be better with less number of plug trays, such as seedling height, seedling width, leaf length, leaf width and leaf number. On the other hand, roots (net formation) increased rapidly as the number of plug trays increased. After 60 days, the matured seedling rate was good at 75.5 ± 8.4% for the 200 plug tray and 72.5 ± 4.1% for the 162 plug tray. The net formation ratio of matured seedling was the best in 60 days of seeding in 162 plug trays. The rooting rate was 98.0 ± 2.1 - 99.3 ± 1.2% when seeded for 60 days or more regardless of the type of plug tray. Conclusion : In order to produce efficient and stable seedlings in the cultivation of Atractylodes macrocephala in Gangwon area, it was considered to be advantageous for 60 days of seedling settling in the plug trays of more than 162 and less than 200.

Background : Atractylodes macrocephala is a perennial herbaceous plant belonging to the family Asteraceae and should be cultivated in field soils with good water dripping due to plant characteristics. However, cultivating farmers mainly have recently been cultivated in paddy soil due to their regional characteristics, which causes the decrease in yield due to poor drainage. Therefore, this study was carried out to investigate the cultivation in high ridge and subsoil breaking effect for stable paddy soils cultivation technology of A. macrocephala. Methods and Results : Soil was paddy soils in the fall of 2017, and the pH (1 : 5) was 6.61 ± 0.15 as a result of chemical and physical properties. EC was 0.49 ± 0.05 dS/m, and organic matter content was 28.69 ± 69 g/㎏ and effective phosphoric acid was 306 ± 17.8 ㎎/㎏. As a result of the soil layering survey, the surface layer was 0 - 26 ㎝ deep as paddy soil mounded with sandy loam in the past. In the surface layer, there was a light layer after 17 ㎝ depth, and volume density was 1.71 ± 1.3 g/㎤. The porosity of the plow pan was 33.41 ± 2.34%. The cultivation methods were cultivation in high ridge (30 ㎝ or more) and level row (10 ㎝ or less) at 2 levels and 3 repetitions. In subsoil breaking, the depth of the plow pan was increased from 17.1 ± 0.5 ㎝ before treatment to 31.1 ± 3.6 ㎝ after treatment and the hardness was 24.8 ± 1.5 ㎜. In the case of rotary plowing, the depth of the plow pan was 17.1 ± 1.9 ㎝ before treatment and 26 ± 2.4 ㎝ after treatment and the hardness was 25.8 ± 2.9 ㎜. The medium growth characteristics of A. macrocephala per treatment showed the tendency of increase in plant length, culm length, number of nodes, number of leaves, and fresh weight in level row cultivation after subsoil breaking. Root growth of cultivation in high ridge after subsoil breaking tended to be good with rhizome weight of 11.6 g per hill. The survival percentages were 98.8 - 100% and the bolting rate was 93.4 - 96.2% Conclusion : In cultivation in high ridge after subsoil breaking in the paddy field of Gangwon area, the decrease of yield of A. macrocephala due to drainage was expected to be alleviated, but final conclusion should be drawn after analyzing soil temperature and soil moisture data.