본 논문은 남해안 자란만 패류양식어장에서 약 2년 동안 월별로 기초생산력, Chl. a, 영양염류, 입자유기물질과 퇴적물의 유기오 염 정도 및 생화학 조성 등 주요 양식생물의 서식환경인자의 변동특성과 상관성 등을 분석하였다. 또한, 다른 연안 어장과 기초생산력을 비교하고 어장환경관리와 관련된 정책방안을 제시하였다. 월별 평균 기초생산력은 6.43∼115.43 ㎎C m-2 hr-1 범위로 여름과 가을에 높았는 데, 가막만과 마산만 보다는 낮았고, 가로림만과 서해보다는 높았으며, 대체적으로 양식장이 많이 분포한 내만은 그 변동 폭이 상대적으 로 컸다. Chl. a를 구성하는 식물플랑크톤의 크기별 점유율이 시기별로 다소 차이가 있었고, 영양염의 고갈로 인한 식물플랑크톤의 생산력 제한은 거의 없었으나, 대부분 시기에 N/P비가 16 이하로 질소가 상대적으로 부족한 것으로 판단되었다. 수층 입자유기물질의 생화학적 조성은 탄수화물이 가장 높았으나, 반면 표층 퇴적물에서는 지질과 단백질 함량이 높았다. 퇴적물의 TOC와 AVS 농도는 만 안쪽에서 높 았고 일부 시기에는 어장환경기준을 초과한 상태였으며, C:N 비는 평균 8.1∼10.4 범위로 나타났다. 기초생산력은 Chl. a와의 상관성이 가 장 높았고, 입자물질성분 중에서는 탄소보다는 질소 및 단백질과의 상관성이 높았다. 최근 5년 동안의 수층에서의 Chl. a, DIN, DIP 농도는 감소하는 경향이었지만, 반대로 퇴적물의 오염도는 증가하는 추세였다. 자란만의 연간 기초생산력 125.9 gC m-2 yr-1, 굴 양식장 면적 4.97 ㎞2 를 고려하면 연간 식물플랑크톤으로부터 생산되는 탄소량이 약 625 ton이며, 연간 굴 생산 습중량은 약 6,250 ton으로 추정되었다.

식중독 세균에 의한 biofilm 형성을 억제하는 효과를 시 험하기 위하여 cinnamon, clove 및 lemongrass 정유의 휘 발성 성분을 분석하였다. 또한 정유의 주요 항균활성 성 분이 polyethylene과 stainless steel 표면에서 식중독 세균 에 의한 biofilm 형성을 억제하는 효과에 대하여 조사하였 다. Cinnamon 정유의 주요 휘발성 성분은 cinnamaldehyde (38.30%), linalool (9.61%), β–caryophyllene (8.90%) 및 1,3,4-eugenol (8.19%)로 동정되었다. Clove 정유의 주요 휘발성 성분은 1,3,4-eugenol (61.84%)로 분석되었다. Lemongrass의 주요 휘발성 성분은 citral의 이성질체인 geranial (19.11%)과 neral (19.23%)로 검출되었으며, citral 은 isomeric acyclic monoterpene aldehydes로서 geranial (trans-citral, 19.11%)과 neral (cis-citral, 19.23%)의 혼합물 로 분석되었다. Cinnamon, clove 및 lemongrass의 주요 성 분 중 cinnamaldehyde, linalool, eugenol 및 citral이 disc diffusion assay에 의해 시험한 6종의 식중독 세균에 대하 여 강한 항균활성을 나타냈다. Eugenol (0.1%)과 citral은 polyethylene 및 stainless steel coupon 표면에서 식중독 세 균에 의해 형성된 biofilm에 대하여 강한 억제 작용을 나 타났다. Cinnamaldehyde (0.1%)는 Listeria monocytogenes ATCC 19112와 Staphylococcus aureus KCCM 11812에 의 해 형성된 biofilm에 대해 장한 억제 작용을 나타냈다. 연 구 결과 cinnamaldehyde, eugenol 및 citral 처리에 의해 식 중독 세균에 의한 biofilm 형성을 억제가 가능할 것으로 판단된다.

Hepatic diseases are divided into two types: alcoholic and non-alcoholic. Non-alcoholic liver injury finally induces fatty liver and damages liver function. Many studies have demonstrated that Ecklonia stolonifera has antioxidative, antiinflammatory, and hepatoprotective activities. We conducted a 12-week double-blind, placebo-controlled, randomized trial to examine the efficacy of E. stolonifera extracts (ESE) on biochemical markers of hepatic function. Sixty-five subjects with mild or moderate liver injuries were randomly allocated to receive either 420 mg/d of ESE or a placebo for 12 weeks. Fifty-five participants completed the trial. No significant adverse events were observed among the subjects during the study. The primary end points were changes in plasma levels of aspartate transaminase (AST), alanine transaminase (ALT), and γ-glutamyltransferase (γ-GT). The secondary end points were changes in lipid profile levels, including total cholesterol (TC), triglyceride (TG), highdensity lipoprotein cholesterol (HDL), and low-density lipoprotein cholesterol (LDL). Compared with the baseline, AST and ALT levels decreased significantly in the ESE group compared to those in the placebo group (P<0.001). In addition, γ-GT levels in the ESE group were significantly lower than those in the placebo group (P=0.016). There were no differences in the TC, TG, HDL, and LDL levels between groups. In conclusion, ESE consumption for 12 weeks improved liver parameters in subjects with liver injury. Regular consumption of ESE could maintain liver health in individuals at risk of hepatic damage.

본 연구는 권취식 측창을 갖는 아치형 단동 플라스틱 온실 내 강제환기장치 설치 및 운용, 환기 성능 개선방안 등을 제안 하기 위해 온실 내부에 유동팬과 배기팬을 설치하여 강제환기 장치 사용에 따른 온실 내부기온 강하 특성을 정량적으로 조 사하였다. 시험은 3가지 환기 조건(측창, 측창+순환팬, 측창+ 순환팬+배기팬)에서 수행되었다. 각 조건 데이터로거를 이용 하여 환기 시작과 동시에 온실 내외부 기온 및 외부환경 변화 를 측정·기록하였고, 환기 방식별 기온차 변화의 평균값으로 부터 정규기온차를 계산하여 기온 강하 효과를 비교하였다. 오전(11:00－12:00)에는 환기 방식에 상관없이 환기 초반 정 규기온차가 일시적으로 증가했다가 감소하는 것으로 나타났 다. 강제환기장치가 더 많이 사용될수록 최대 정규기온차는 1.158에서 1.037로 감소하였고 최대 정규기온차에 도달하는 시간도 340초에서 110초로 단축되었다. 강제환기장치의 사 용은 정규기온차가 0.8까지 감소하는데 소요된 시간을 1,030 초에서 550초로, 0.6까지는 1,610초에서 915초로, 0.4까지는 2,315초에서 1,360초로, 자연환기의 약 60% 수준으로 감소 시켰다. 오후(14:00－15:00)에는 정규기온차의 증가가 관측되지 않았지만, 환기 시작과 동시에 기온차가 감소하기 시작 했다. 또한 강제환기장치가 더 많이 사용될수록 정규기온차 가 0.8까지 내려가는 시간을 560초에서 345초로, 0.6까지는 825초에서 540초로, 0.4까지는 560초에서 345초로, 약 70% 수준으로 감소시켰다. 따라서, 보다 효과적이고 경제적인 환 기를 위해 강제환기장치는 오전과 같이 열부하가 높은 환경에 서 적극적으로 사용하는 것이 바람직하다고 판단된다.

We investigated physicochemical properties and isotopic compositions of organic matter (δ13CTOC and δ15NTN) in the old fish farming (OFF) site after the cessation of aquaculture farming. Based on this approach, our objective is to determine the organic matter origin and their relative contributions preserved at sediments of fish farming. Temporal and spatial distribution of particulate and sinking organic matter (OFF sites: 2.0 to 3.3 mg L-1 for particulate matter concentration, 18.8 to 246.6 g m-2 day-1 for sinking organic matter rate, control sites: 2.0 to 3.5 mg L-1 for particulate matter concentration, 25.5 to 129.4 g m-2 day-1 for sinking organic matter rate) between both sites showed significant difference along seasonal precipitations. In contrast to variations of δ13CTOC and δ15NTN values at water columns, these isotopic compositions (OFF sites: -21.5‰ to - 20.4‰ for δ13CTOC, 6.0‰ to 7.6‰ for δ15NTN, control sites: - 21.6‰ to - 21.0‰ for δ13CTOC, 6.6‰ to 8.0‰ for δ15NTN) investigated at sediments have distinctive isotopic patterns (p<0.05) for seawater-derived nitrogen sources, indicating the increased input of aquaculture-derived sources (e.g., fish fecal). With respect to past fish farming activities, representative sources (e.g., fish fecal and algae) between both sites showed significant difference (p<0.05), confirming predominant contribution (55.9±4.6%) of fish fecal within OFF sites. Thus, our results may determine specific controlling factor for sustainable use of fish farming sites by estimating the discriminative contributions of organic matter between both sites.

Fisheries is known as a high-risk industry in Korea, and various efforts have been made to reduce occupational accidents. Trap fisheries represent crustacean production, accounting for 4.7% of total fisheries production and 10.7% of its production value, which is classified as a relatively high-risk industry. With the disaster insurance payment data of the National Federation of Fisheries Cooperatives (NFFC) from 2016 to 2020, the accident rate of the entire fishery, the accident rate of trap fisheries, and the type of disasters in the past five years were analyzed. As a result, the average fishery accident rate for the past five years was 5.31%, but it was high at 6.15% for coastal trap fisheries and 5.59% for offshore trap fisheries. Slips and trips, struck by objects and contact with machinery were the most common types of the accident according to the characteristics of the work, and hand injuries were analyzed the most. Additional efforts, including education for accident prevention, development of personal protective equipment and improvement of the working environment, are needed to prevent accidents caused by repeated types of disasters.

This study was carried out to investigate the effect of side vent heights on temperature and relative humidity inside and outside the single-span plastic greenhouse (W: 7 m, L: 40 m H: 3.9 m) during natural ventilation. Four different heights (120, 100, 80, 60 cm) of the side vent were used as an experimental condition. Variations of temperature and relative humidity inside and outside the greenhouse and the differences between heights were compared by using one-way ANOVA. In the daytime, the difference in temperature between inside and outside the greenhouse was dropped from 14.0°C to 7.1°C as the side vent height increased. The temperature difference in the nighttime was less than 0.2°C regardless of the height. One-way ANOVA on the temperature difference between heights presented that the statistical significance was founded between all of the combinations of height in the daytime. The difference in relative humidity between inside and outside the greenhouse was grown from –13.8% to – 22.2% with a decrease in the side vent height. The humidity difference in the nighttime was less than 1% regardless of the height. One-way ANOVA on the humidity difference revealed that most of the side vent heights showed significance in the daytime but between 100 and 80 cm was not significant. It seemed because the external air became cooler during the experiment with a height of 80 cm. Conclusively, this study empirically demonstrated that the higher side vents resulted in the decrease of differences in temperature and relative humidity between inside and outside the greenhouse, and also the effect of side vent height was statistically significant. This study may be helpful for deciding the height of the side vent effective for controlling temperature and relative humidity in a single-span greenhouse during natural ventilation.

Known for its effectiveness in weight loss and diabetes prevention, Gymnema sylvestre products can be found in the US, Japanese, and Indian markets. However, the recommended dosage or safety of these products has not yet been proven. Therefore, development of an analytical method for detecting the content of Gymnema sylvestre in food products is required. Accordingly, this study proposes an analysis method that can examine Gymnema sylvestre in food using LC-ESI-MS/MS and KASP (Kompetitive Allele-Specific PCR) markers. In LC-ESI-MS/MS, a simultaneous analysis method for gymnemic acid and deacylgymnemic acid was optimized using negative ionization mode, and its validation test was completed for solid and liquid samples. In addition, KASP markers were prepared by finding the specific SNP of G. sylvestre in ITS2 and matK through DNA barcodes. The two KASP markers returned positive FAM fluorescence result when combined with G. sylvestre, and this aspect was confirmed in raw G. sylvestre as well. The applicability of the method was tested on 21 different food and healthy functional products containing G. sylvestre purchased on the internet. As a result, although there was a difference in the ratios of gymnemic acid and deacylgymnemic acid in LC-ESI-MS/MS, the index component was detected in all 21 products samples. In the KASP analysis, 9 products returned positive FAM result, and the rest of the products were found to be containing G. sylvestre extract. This study is the first study to use the dual system of LC-ESI-MS/MS and KASP for the analysis of G. sylvestre. The study has confirmed that these two methods are applicable to the examine G. sylvestre content in food products.

This study analyzed the wake characteristics of the rim-driven propeller (RDP) used in an underwater robot. For underwater robots to perform specific missions, not only propulsion characteristics but also wake characteristics must be considered. In this study, a blade was designed based on NAC 0012 with a symmetrical cross-section. The RDP was hubless with three or four blades. The influence of both the free water surface and the bottom was considered, and the wake was measured using a particle image velocimetry in the advance ratio of 0.2 to 1. Model 1 showed symmetrical wakes in the entire advance ratio section. Model 2 showed asymmetric wakes due to the influence of the free water surface and the bottom at low advance ratio.

Fishery sea anchor is widely used for many fishing vessels. However, standardization of the dimension and terminology of the fishery sea anchor has not been achieved, reducing the reliability of the performance and safety of the fishery sea anchor. Therefore, this study researches the reality of usage of fishery sea anchor and then attempts to suggest the development direction of the standard draft as basic data. As a result of the survey on the reality of usage show that various terms were used for each part of the fishery sea anchor (including the incorrect form of non-standard words derived from Japanese) and that the production of this product in the fishery sea anchor market was concentrated on one specific manufacturer. In addition, the main specifications of the fishery sea anchor are set and manufactured based on conventional experience without standards. In the field, there was no standardized drawing for fishery sea anchor and users had low awareness of the main specifications of fishery sea anchor. Therefore, this study suggested the following regarding the fishery sea anchor: standard terms for each part including Korean and English names and standard drawing of sea anchor. It is hoped that this study will contribute to research for the standardization of fishery sea anchor, which will increase in reliability and lead to increased interest in standardization in the fishery field.

PURPOSES : Steel deck bridges are the preferred structural type for reducing dead load, and the use of thin-layer asphalt concrete with excellent adhesion to the steel deck and excellent deformation followability is increasing for bridge pavements. However, because these materials are constructed at a high temperature of 240 °C or higher to maintain high fluidity during construction, excessive thermal deformation and stress may be temporarily induced in the steel deck. Therefore, the stability of the structure must be assessed by considering the environmental conditions of the site during pavement construction. Herein, a method is presented for estimating the heat source equation, in which conduction and convection effects are removed using temperature measurement data, for modeling U-rib using plate elements. The validity of the study is assessed by deriving the equivalent heat source equation using the temperature data measured from the underside of the steel deck while constructing a 40-mm-thick goose asphalt concrete pavement layer on a 12-mm-thick steel deck. In addition, the practicality is verified by performing heat transfer and thermal stress analyses. METHODS : By comparing the temperature data measured during the construction of high-fluidity asphalt concrete with the results of repeated heat transfer numerical analysis, heat source data without field conduction and convection conditions are obtained. Subsequently, a heat source equation suitable for the heat source data is derived using the least-squares method. RESULTS : The results of the heat transfer analysis using the equivalent heat source equation calculated using the presented method are almost consistent with the measured temperature data. In addition, the behavioral characteristics of the structure that matches the behavior of the actual structure can be derived through thermal stress analysis, which considers heat conduction and convection to adjacent members. CONCLUSIONS : Even when the steel deck and U-rib member are modeled as plate elements, thermal effect analysis can be performed reasonably while considering field conditions.

PURPOSES : In this study, the results of an intensive field survey of a composite pavement applied to an expressway in Korea are analyzed. The results are intended to be used as basic data for evaluating the composite pavement and for preparing quality control measures through a review and analysis of the change in the state of the tensile bonding strength of the composite pavement. METHODS : To evaluate, repeated field coring and indoor tensile bonding strength evaluations are conducted for five years, and the commonality evaluation of pavement using pavement condition investigation equipment is conducted annually. RESULTS : The results of this study show that the degree of reflective crack generation varies depending on the type of concrete pavement and type of asphalt mixture applied to the intermediate layer, and that the CRCP composite pavement exhibits better initial commonality than the JCP composite pavement. In addition, it is confirmed that the composite cross-section pavement section with MASTIC applied as an intermediate layer shows excellent adhesion performance between the lower concrete layers from the beginning of construction in all sections, as well as satisfied the quality control standards for good pavement and tensile adhesion even after approximately five years of public use. CONCLUSIONS : Based on a follow-up survey of the composite pavement testing sections, it is confirmed that the section constructed with the MASTIC intermediate layer and CRCP satisfies the quality control standards for good pavement conditions and tensile adhesion even after approximately five years of public use.

본 연구는 액티브 시니어 골프참여자를 대상으로 지각된 환경적요인과 주관적 건강 및 활동적 노화에는 어떠한 관계가 있는지 규명하는데 목적이 있다. 본 연구의 목적을 달성하기 위하여 SPSS(Statistical Package for the Social Sciences) 18.0과 AMOS(Analysis of Moment Structures) 18.0을 이용하여 빈도분석, 확인적 요인분석, 신뢰도분석과 상관관계분석을 실시하였고 모형을 설정한 뒤 구조방 적식모형(Structural Equation Modeling)을 통하여 변인 간의 인과적 관계를 규명하였다. 이상과 같은 연 구방법과 연구모형 검증을 기초로 하여 본 연구에서 도출된 결과는 다음과 같다. 첫째, 지각된 환경적요인 은 활동적 노화에 정적인 영향을 미치는 것으로 나타났다. 둘째, 활동적 노화는 주관적 건강에 정적인 영향 을 미치는 것으로 나타났다. 셋째, 지각된 환경적요인은 주관적 건강에 정적인 영향을 미치는 것으로 나타 났다. 넷째, 지각된 환경적요인과 주관적 건강의 관계에서 활동적 노화는 매개하는 것으로 나타났다.

본 연구에서는 어류가두리양식장 시설의 재배치 이후 기존 양식장 아래 퇴적물의 회복상태를 규명하고자 하였으며, 이를 위하 여 양식장 아래 퇴적물과 저서다모류 군집 조사를 수행하였다. 양식장 철거 이전인 2017년 10월에 사전 조사를 수행하였으며, 양식장 철 거 이후인 2017년 11월부터 2018년 10월까지는 매달, 이후 2020년 10월까지는 2 ~ 3달 간격으로 조사하였다. 조사 정점은 철거된 양식장 위 치에 3개 정점(Farm1 ~ 3)과 양식시설물이 없는 주변 해역에 3개의 대조 정점(Con1 ~ 3)으로 선정하였다. 사전 조사에서 기존 양식장의 총유 기탄소(평균 22.67 mg․g-1 dry weight)는 대조 정점(평균 13.68 mg․g-1 dry weight)보다 높았으나, 양식장 철거 이후 점차 감소하여 약 1년 이 후에는 통계적으로 유의한 차이를 보이지 않았다(p<0.05). 저서다모류 군집은 여름철 기존 정점에서 무생물 군집이 출현하였으며, 무생물 시기 이후 오염지시종인 Capitella capitata 단일종이 극우점하는 낮은 다양도의 군집으로 천이하였다. 다음해 여름철 무생물 시기 이전까지 종다양도가 증가하고 오염지시종의 비율이 감소하여 저서다모류 군집이 회복되는 경향을 나타내었으며, 이러한 변화는 매년 반복되었다. 연구 지역은 양식장 아래의 지형학적인 특성으로 인하여 매년 무생물 군집이 출현하고 있으나, 조사가 진행될수록 무생물 발생 기간은 짧아지고, 군집이 회복되는 과정은 빠르게 진행되었다. 어류 양식장의 이전 후 기존 양식장 정점의 퇴적물은 생물학적인 회복이 여전히 진행 중이며, 추가적인 모니터링을 통해 회복의 경향을 연구할 필요가 있다.

An ecological niche is defined as the specific role of a species influenced by time, space, and other resources. By investigating overlaps between ecological niches of different species, we could estimate the degrees of interspecific competition. Such studies often use geographic information systems (GIS) to discover niche overlaps between species. In this study, we used GIS to estimate the spatial niches of two Korean frog species (Pelophylax nigromaculatus and P. chosenicus). This enabled us to predict their geographic distributions in order to identify their coexistence regions and distribution patterns. The results confirmed that altitude was an important variable for predicting their distribution, with a correlation with their climatic range. Spatial distributions of the two frog species were highly overlapped, as the distribution range for P. nigromaculatus included most of the range of P. chosenicus, showing a sympatric distribution pattern. Within the coexisting regions, however, the presence sites for the two species did not overlap, implying weak competition. To confirm the principal factors influencing their competitive relationship and reasons for their sympatric distribution pattern, we need more detailed in-depth studies on the diverse environmental variables within the regions where the two species coexist. By doing so, we would be able to identify various mechanisms for avoiding competition in sympatric frog species.

In this study, an accelerated weathering test was performed to examine the variation of thermal insulation performance according to the service life. A widely used class 1 thermal screen (matt georgette + polyethylene (PE) foam + chemical cotton + felt + matt georgette) was selected as the target thermal screen. The ultraviolet irradiation that reached the target thermal screen specimen (60 x 60cm) was 5mW/cm2. Thus, the ultraviolet irradiance was set to 5mW/cm2, and the exposure periods of accelerated weathering conditions on the specimens were set to 0, 282, 847, and 1412h. The radiation exposure periods of the weathering conditions for 0, 282, 847, and 1412h indicate the amount of ultraviolet accumulation for 0, 1, 3, and 5years, respectively. In the accelerated weathering test, the target specimens that completed each exposure phase were subjected to the hotbox test to analyze their thermal insulation performances. Consequently, the thermal insulation performance of the multi-layer thermal screen was estimated to degrade rapidly after approximately two years. In the accelerated weathering condition, a quadratic function model was used to calculate the expected service life, since it adequately described the variation in thermal insulation of the thermal screen according to time. The results showed that when the thermal insulation performance degraded by 5, 10, 20, and 30%, the expected service lives were 2.5, 3.3, 4.5, and 5.5years, respectively.

The efficient nutrient solution cooling system consisting of buffer tank, three-way valve, and heat pump et al. was developed for hydroponics of leafy vegetables at high temperatures period. It was designed in such a way that the buffer tank was first cooled to enable precise temperature control of the nutrient solution with small capacity heat pump. Developed system was installed in the NFT hydroponics farm cultivating leafy vegetables and performance evaluation was carried out. For cultivation of Lalique lettuce(Lactuca sativa var.), the temperature of nutrient solution tank, buffer tank, and the root zone, the performance of cooling system, and the environment of greenhouse were analyzed. Also, by measuring the growth and yield of lettuce, the effect of cooling nutrient solution on the growth of lettuce NFT hydroponics was analyzed.