한반도에 분포하는 백악기 육성퇴적층 중 경상누층군 퇴적물의 기원지 조성과 조구조 환경에 대한 연구는 활발 하게 이루어진 반면에 그 외 퇴적층에 대한 사암의 암석학적 연구는 아직 부족하다. 본 연구에서는 부산 기장군 일광 읍 신평리 해안가 일대에 분포하는 백악기 이천리층의 사암을 대상으로 암석기재학적 연구를 수행하여 기원지의 특성 에 대한 예비 해석 결과를 보고하였다. 이천리층 사암은 미성숙한 조성과 조직을 나타내며 백악기 당시 유라시아 동쪽 연변부에서 일어난 고태평양판의 섭입에 의해 융기하여 발달한 화산호로부터 단시간에 퇴적물이 생성되어 운반된 것으 로 보인다. 그리고 사암 내 부수광물로 크롬 첨정석이 다수 관찰되는데, 이는 기원지에 초고철질암이 분포하였음을 의 미한다. 향후 추가 연구를 바탕으로 크롬 첨정석 기원암의 형성 환경 뿐만 아니라 이천리층 퇴적 당시 경상분지 동남 부의 조구조 환경에 대한 해석과 경상누층군과의 층서 대비가 가능할 것으로 기대된다.

해양을 이용하려는 수요자 증가에 따라, 해양공간을 합리적으로 배분하기 위해 해양공간계획(Marine Spatial Planning)제도가 도 입되었다. 이 중 어업활동보호구역은 수산자원보호 등 어업활동 보호를 위해 지정되었다. 그러나, 어업활동보호구역을 주로 항해하는 어 선은 우리나라에서 발생하는 해양사고의 약 70 %를 차지할 정도로 위험에 노출되어 관리가 필요하다. 본 연구의 목적은 어업활동보호구 역 내 어선 통항 안전 확보를 위해 어업활동보호구역과 항만·항행구역에서 발생하는 해양사고를 분석하는 것이다. 이를 위해 해양용도구 역의 교통량을 조사하고 선종별, 사고 유형별, 톤수별, 사고원인별, 인명피해별로 해양사고를 조사하였다. 분석 결과 각 유형별 단위 면적 당 해양사고는 대부분 항만·항행구역에서 더 많이 발생하였으나 전체적으로 발생한 해양사고는 어업활동보호구역이 높았다. 특히 인명사 고가 많이 발생하여 통항 안전관리가 필요한 것으로 식별되었다.

The objective of this study is to evaluate the antioxidant components, elastase inhibition activities, and melanin synthesis rates of Korean traditional Actinidia (Actinidia arguta) fruits and leaves depending on the ethanol extraction concentrations. The total polyphenol content was the highest in the 50% ethanol extract of both fruits and leaves, with values of 634.1 mg GAE/100 g and 3,985.2 mg GAE/100 g, respectively. The total flavonoid content was the highest in the fruit 90% extract and leaf 50% extract at 191.9 mg/100 g and 2655.6 mg/100 g, respectively. The vitamin C content was the highest in the 50% extract of leaves at 2990.3 mg/100 g. Elastase inhibition was the highest at 56.9% in the leaf 50% extract at a concentration of 1,000 μg/mL. Melanin synthesis inhibition showed the highest melanin synthesis inhibitory effect among the extracts, as the leaf 50% extract showed an inhibitory rate of 65% or more. Therefore, the antioxidant components, elastase inhibition activities, and melanin synthesis inhibitory rate were better in leaves than in fruits. The leaf 50% extract was particularly the best among the extracts. Korean traditional Actinidia leaves can be considered as potential sources for new functional materials.

본 연구는 후박나무 용기묘를 대상으로 전광, 35%, 55%, 75% 차광수준에 따른 생장 특성 및 생리적 반응을 알아보고자 실시하였다. 후박나무 묘목은 차광 시 상대적으로 지상부의 생장특성을 보여주는 지표들이 높은 경향을 보였으며, 전광에서는 뿌리와 관련된 지표인 뿌리건중비, 뿌리생장에 서 유의적으로 높은 경향을 보였다. 또한, 전광에서의 낮은 광보상점과 높은 순양자수율은 이미 후박나무가 내음성을 가지고 있고, 광이 부족한 환경에 노출된 차광처리구들에서 암호흡량의 감소, PIABS 및 SFIABS의 증가로 광합성 기구의 활력을 효율적으로 증가시키는 것을 알 수 있다. 한편, 전광은 지상부 비율의 증가, 낮은 T/R율과 가장 높은 순광합성속도를 보여 광합성으로 합성되는 탄수화물이 지상부 뿐만 아니라 지하부의 생장 역시 증가시킨 것을 알 수 있는 결과였다. 따라서 후박나무 용기묘는 전광조건에서 생장 및 생리적 특성이 가장 양호한 경향을 보였다.

Cyanobacterial resting cells, such as akinetes, are important seed cells for cyanobacteria’s early development and bloom. Due to their importance, various methods have been attempted to isolate resting cells present in the sediment. Ludox is a solution mainly used for cell separation in marine sediments, but finding an accurate method for use in freshwater is difficult. This study compared the two most commonly used Ludox methods (direct sediment treatment and sediment distilled water suspension treatment). Furthermore, we proposed a highly efficient method for isolating cyanobacterial resting cells and eDNA amplification from freshwater sediments. Most of the resting cells found in the sediment were akinete to the Nostocale and were similar to those of Dolichospermum, Cylindrospermum, and Aphanizomenon. Twenty times more akinetes were found in the conical tube column using the sediment that had no treatment than in the sample treated by suspending the sediment in distilled water. Akinete separated through Ludox were mainly spread over the upper and lower layers in the column rather than concentrated at a specific depth in the column layer. The mibC, Geo, and 16S rDNA genes were successfully amplified using the sediment directly in the sample. However, the amplification products of all genes were not found in the sample in which the sediment was suspended in distilled water. Therefore, 5 g to 10 g of sediment is used without pretreatment when isolating cyanobacterial resting cells from freshwater sediment. Cell isolation and gene amplification efficiency are high when four times the volume of Ludox is added. The Ludox treatment method presented in this study isolates cyanobacterial resting cells in freshwater sediment, and the same efficiency may not appear in other biotas. Therefore, to apply Ludox to the separation of other biotas, it is necessary to conduct a pre-experiment to determine the sediment pretreatment method and the water layer where the target organism exists.

This study compares the abundance and community structure of zooplankton organisms from pelagic regions, and considers particularly the trophic levels vs. zooplankton abundances and biomass. Zooplankton samples were collected three times from May to November 2022, at 30 temperate lakes and reservoirs, which belong to four different river basins. The total zooplankton abundance, biomass and species index were showed considerable spatial variation. The spatial pattern of rotifer abundance was similar to that of total zooplankton abundance, while there were not showed similar patterns of zooplankton biomass (μg L-1) in lentic ecosystems. The rotifer strongly dominated the zooplankton assemblage in smaller lentic system than that of larger. A total of 130 species of zooplankton were identified (83 rotifers, 34 cladocerans and 13 copepods). The total average of zooplankton abundance and biomass were 213.7±342.3 Ind. L-1 (n=129) and 1382.8±1850.4 μg L-1, respectively. Total and average of zooplankton abundance were usually dominated by the rotifers (>56.9%), while those of zooplankton biomass were dominated by the cladocerans and copepods (>73.6%) in lentic ecosystems. Considering the Trophic State Index (TSI), the factors of zooplankton abundance and biomass were included in between meso- and eutrophic states (27 lakes, 90% of all). The mean abundance and biomass of zooplankton in eutrophic systems were higher than that of meso- and hypertrophic systems. From this result, we suggest that management strategy for the lentic ecosystem water environment has to be focused more on small-sized lakes and reservoirs, in terms of zooplankton assemblages.

Recently, Korean government has introduced Multi Metric Indices (MMI) using various biocommunity information for aquatic ecosystem monitoring and ecosystem health assessment at the national level. MMI is a key tool in national ecosystem health assessment programs. The MMI consists of indices that respond to different target environmental factors, including environmental disturbance (e.g. nutrients, hydrological and hydraulic situation of site etc.). We used zooplankton community information collected from Korean lakes to estimate the availability of candidate zooplankton MMI indices that can be used to assess lake ecosystem health. First, we modified the candidate indices proposed by the U.S. EPA to suit Korean conditions. The modified indices were subjected to individual index suitability analysis, correlation analysis with environmental variables, and redundancy analysis among indices, and 19 indices were finally selected. Taxonomic diversity was suggested to be an important indicator for all three taxonomic groups (cladoceran, copepod, rotifer), on the other hand, the indices using biomass for large cladocerans and copepods, while the indices using abundance were suggested for small cladocerans and rotifers.

Environmental DNA (eDNA) can exist in both intracellular and extracellular forms in natural ecosystems. When targeting harmful cyanobacteria, extracellular eDNA indicates the presence of traces of cyanobacteria, while intracellular eDNA indicates the potential for cyanobacteria to occur. However, identifying the “actual” potential for harmful cyanobacteria to occur is difficult using the existing sediment eDNA analysis method, which uses silica beads and cannot distinguish between these two forms of eDNA. This study analyzes the applicability of a density gradient centrifugation method (Ludox method) that can selectively analyze intracellular eDNA in sediment to overcome the limitations of conventional sediment eDNA analysis. PCR was used to amplify the extracted eDNA based on the two different methods, and the relative amount of gene amplification was compared using electrophoresis and Image J application. While the conventional bead beating method uses sediment as it is to extract eDNA, it is unknown whether the mic gene amplified from eDNA exists in the cyanobacterial cell or only outside of the cell. However, since the Ludox method concentrates the intracellular eDNA of the sediment through filtration and density gradient, only the mic gene present in the cyanobacteria cells could be amplified. Furthermore, the bead beating method can analyze up to 1 g of sediment at a time, whereas the Ludox method can analyze 5 g to 30 g at a time. This gram of sediments makes it possible to search for even a small amount of mic gene that cannot be searched by conventional bead beating method. In this study, the Ludox method secured sufficient intracellular gene concentration and clearly distinguished intracellular and extracellular eDNA, enabling more accurate and detailed potential analysis. By using the Ludox method for environmental RNA expression and next-generation sequencing (NGS) of harmful cyanobacteria in the sediment, it will be possible to analyze the potential more realistically.

This experiment was conducted to confirm the possibility of preparing Sorghum×sudangrass hybrid artificial hay using far-infrared rays in Korea. The machine used in this experiment is a drying device based on far-infrared rays, and is designed to control temperature, air flow rate, far-infrared radiation amount, and air flow speed. The Sorghum×sudangrass hybrids harvested in late September were wilted in the field for one day, and a drying test was performed on them. Conditions for drying were performed by selecting a total of 7 conditions, and each condition induced a change in radiation amount in a single condition (42%) and two steps (4 treatments) and three steps (2 treatments). The speed of the air flow in the device was fixed at 60 m/s, and the run time was changed to 30, 60, and 90 minutes. The average dry matter (DM) content was 82.84%. The DM content was 59.94 and 76.91%, respectively, in drying conditions 1 and 3, which were not suitable for hay. In terms of drying rate, it was significantly higher than 80% in the 5, 6 and 7 treatment, and power consumption was slightly high with an average of 5.7 kw/h. As for the feed value according to each drying condition, the crude protein (CP) content increased as the drying time increased, and there was no significant difference between treatments in ADF, NDF, IVDMD and TDN content. In terms of RFV, treatment 1, which is a single condition, was significantly lower than the complex condition. Through the above results, it was determined that the drying conditions 4 and 5 were the most advantageous when considering the drying speed, power consumption, and quality.

Broad bean wilt virus 2 (BBWV2) is a species in the genus Fabavirus and family Secoviridae, which is transmitted by aphids and has a wide host range. The BBWV2 genome is composed of two single-stranded, positive-sense RNAs, RNA-1 and RNA-2. The representative symptoms of BBWV2 are mosaic, mottle, vein clearing, wilt, and stunting on leaves, and these symptoms cause economic damage to various crops. In 2019, Perilla fructescens leaves with mosaic and yellowing symptoms were found in Geumsan, South Korea. Reverse-transcription polymerase chain reaction (RTPCR) was performed with specific primers for 10 reported viruses, including BBWV2, to identify the causal virus, and the results were positive for BBWV2. To characterize a BBWV2 isolate (BBWV2-GS-PF) from symptomatic P. fructescens, genetic analysis and pathogenicity tests were performed. The complete genomic sequences of RNA-1 and RNA-2 of BBWV2-GS-PF were phylogenetically distant to the previously reported BBWV2 isolates, with relatively low nucleotide sequence similarities of 76-80%. In the pathogenicity test, unlike most BBWV2 isolates with mild mosaic or mosaic symptoms in peppers, the BBWV2-GS-PF isolate showed typical ring spot symptoms. Considering these results, the BBWV2-GS-PF isolate from P. fructescens could be classified as a new strain of BBWV2.

본 연구에서는 석문간척지 주변 농가들을 대상으로 간척지 논 범용화를 위한 암거공법 관련 세부 기술요인에 대한 농가 수요조사 및 분석을 통해 다음과 같은 결과들을 도출하였다. 첫째, 지하흡수공을 보다 필요하고 중요하게 인식할수록 해당 기술에 대한 수용의사가 증가하는 것으로 나타났다. 둘째, 지하배수를 보다 필요하고 중요하게 인식할수록 해당 기술에 대한 수용의사가 증가하는 것으로 나타났다. 셋째, 지하관수를 보다 필요하고 중요하게 인식할수록 해당 기술에 대한 수용의사가 증가하는 것으로 나타났다. 넷째, 심토파쇄를 보다 중요하게 인식할수록 해당 기술에 대한 수용의사가 증가하는 것으로 나타났다. 다섯째, 지하흡수공 기술에 대한 수용의사가 높은 농가들이 무굴착암거공법 수용확률이 높은 것으로 나타났다.