The number 01' patien ts with tongue carcinoma is increasing rapidly among young indiv idua ls in many parts of the worl d. Until now‘ most of studies were focused on the comparison malignancy wi th normal 01' dysplasia. There is little report of gene a lterations in normal to cancer , oral carcinogenesis. The purpose of present study is to evaluate the gene a ltc ration in every steps of oral carcinogenes is by DD- PCR. To induce tongue carcinoma in rat by 4- NQO. each dri nking water made 10ppm. 25ppm. 50ppm and control(o nly D.W without 4-NQO) . Specimens were classified into 4 groups s uch as co ntrol, I(mild & mocle rate dysplasia) , II(severe dysplasia and car cinoma in s itu) , III(carcinoma). Total RNA was ext racted and DD- PCR was performed using customized random primers. And to confïrmed t he results of DD-PCR‘ RT- PCR a nd real-time PCR with specific primers were carried out. There was phenotypic alteration in tongue 。f dosc a nd t imc dc pcndcnt man ncr. In gross examination, multiple papules, patch form or ulcerations were observed during 4 - NQO t reatment Hi s tologicall y, dysplasia was observed in 3 to 6 month and tumor formation in 6 to 8 month For DD-PCR, RT-PCR and real-time PCR, cyclophilin A, BAC RP23-372MB and BAC CH230-103E9 were differ entia lly expressed. Taken together, cyclophilin A has a role in all steps of oral carcinogenesis. BAC RP23-372N田is implica ted in carcinoma in s itu a nd BAC CH230-103E9 mRNA expression is assoicated with dysplasia and carcinom in s itu Conclus ively. some genes a re impli catcd a ll st eps of oral carci nogenesis, others are associated with one step, whi ch meant that genes are di fferentia lly expressed in every steps

Earthquake resistance design has been developed many countries like Japan, USA, Mexico, New Zealand etc., which countries have experienced many earthquakes. Nowadays, earthquake resistance design has come into worldwide use. In Korea, the seismic design regulations have been established since 1988 in order to minimize the economic losses. Recently performance based design method has been adopted as a new Earthquake resistance design method. These regulations, however, are targeted for newly constructed buildings, In Korea, there are no regulations for existing buildings that built before 1988. So, we need to prepare the regulations that evaluate the seismic performance, furthermore proper retrofitting design guideline needs to be proposed when remodeling old buildings. This study was performed that many existing apartments is being a Remodeling object when considering the present condition of existing apartment and the problerns of cost and environment in the future plan. When Remodeling construction is reviewed by former the Seismic Performance Evaluation Method, generating problems is evaluation by using Push-over. According to this, it provides the appropriate method of calculating the Seismic Performance Index.

LEDs have been shown to be a safe, efficient, light-weight, and less-expensive alternative to heal wound. LED irradiation at the same biostimulatory wavelength of previous laser studies have similar biochemical effects The purpose of present study is to evaluate the effects of wound healing by LED irradiation. Thirty 34-day-old sprague dawley were used for present study. 1.5mm diameter defected holes were formed in both ear lobes of rat by rubber dam punch. 635nm and 890nm irradiation was performed by LED for 2 weeks, followed by histologíc examination staíned with H&E and Masson trichrome. Also, RT-PCR was carried out to find out the mRNA expression level in gingival fjbroblast irradiated by 635nm for 1 hour. In gross exarnination, wound healing was observed in irradiated group comparing to control For microscopic exarnination, repair by connective tissues was filled in defects of irradiated group, while dense cellular bands consisting of fjbroblasts and capi llaries were found at the end of defect in control By staining of masson trichrome, amount of collagens were found in irradiated group. In a result of RT-PCR, mRNA expressions of TGF- ß , MMP-1,3 and Timp-3 were down-regulated in irradiated group comparing with their expression in control group. Taken together, LED irradiation increase the prolifeation and the activity of fibroblasts and down-regualted the TGF-ß , MMP- 1,3 and Timp- 3 mRNA, followed by activation of would healing.

본 연구의 목적은 과학 교사가 중학교 과학 교과서에 제시된 물리 영역 탐구 활동 자료를 평가 하는 기준을 알아보는 것이다. 이를 위해 3가지 유형의 탐구 활동 주제를 선정하여 16명의 과학 교사가 5종의 교과서 탐구 활동 자료를 평가하였다. 수집된 답변은 줄 단위 분석으로 반복적으로 비교하여 코드화하였다. 그 결과, 과학 교사는 교과서 탐구 활동 자료를 평가할 때 다음과 같은 기준을 고려하는 것으로 분석되었다. 첫째, 과학 교과서 탐구 활동 자료의 내·외적 체제로서 내적 체제(탐구 목표, 탐구 준비물, 탐구 과정 및 탐구 내용, 정리하기)와 외적 체제(시각적 자료의 배치, 합리적인 지면 구성)를 고려하였다. 둘째, 학습자 요소, 학교 환경 요소, 교사 요소로 과학 교과서 탐구 활동 자료를 사용하는 사용자 환경을 고려하였다. 본 연구를 바탕으로 다양해지는 과학 교과서에 제시된 탐구 활동 자료를 평가하는 기준을 제시할 수 있다. 과학 교사가 과학 교과서에 제시 된 탐구 활동 자료를 평가하는 데 있어 고려한 개념과 범주들은 추후 각 학교 내 교과서 선정 과정에서 평가 기준으로 사용될 수 있을 것이다. 또한, 교과서 집필 과정에서 탐구 활동 내용을 구성할 때 고려될 수 있을 것이다.

1990년대 초반 다양한 시설물 붕괴사고와 인해 발생하는 국가적인 재산손실과 국민의 안전성 확보를 위한 시설물안전관리 특별법이 재정되었으나 최근 건축 및 토목 구조물 등 30년 이상의 노후 된 시설물의 급속한 증가 및 인건비증가, 공기단축, 민원증가로 P.C구조물의 공사가 증가됨에 따라 P.C구조물을 포함한 안전점검 및 유지관리를 위한 정밀안전진단의 필요도와 중요도는 높아졌으나 수행 기술자의 기술 숙련도에 따라 진단결과와 측정 정밀도에 영향을 미치므로 본 연구에서는 P.C구조물의 안전진단을 위한 3축(X, Y, Z축) 가속도 측정 센서 부착 시 P.C구조물의 구조해석을 통한 모드분석을 수행하여 센서 의 최적 위치 도출에 대한 연구가 필요한 실정이다.

현재 이루어지는 구조물의 안전진단과 성능에 대한 정밀검사는 기술자에 의해 수행되어 측정된 데이터가 경험적 판단에 의해 변화될 수 있는 수준에 머물러 있는 실정으로 본 연구에서는 프리캐스트 콘크리트 구조물을 실제 현장에서 이루어지는 설치방법과 동일하게 설치 후 기존 동적센서와 3축(3측(X, Y, Z축) 가속도 측정 센서 부착하여 손상정도에 따른 가속도 응답과 FFT분석 결과를 비교하여 P.C구조물의 안전진단에 대한 데이터의 불확실성에 대한 개선과 교체시기, 손상예측 등에 대한 기초자료로 활용하기 위함이다.

This study was conducted to propose a strength correction factor to compensate the strength delay at designed age caused by the temperature drop in low temperature condition. Strength correction factors due to temperature drop T28 were provided using the constant function model.

본 연구는 전라남도 여수시 돌산읍에 위치하는 돌산도의 관속식물상을 조사하고 분포가 확인된 주요 식물에 대해 논의하였다. 현지조사는 2017년부터 2018년까지 총 15회에 걸쳐 수행되었으며, 이에 대한 증거표본은 국립수목원 산림생물표본관(KH)에 소장하였다. 분포가 확인된 관속식물은 113과 338속 517종 1아종 49변종 3품종 총 570분류군이다. 이중 한국특산식물은 개족도리풀 등 18분류군을 확인되었으며, 환경부지정 식물구계학적특정식물종은 V-I등급으로 총 90분류군이 조사되었다. 희귀식물은 위기종(EN)인 백양꽃 1분류군을 포함하여 총 4분류군이며, 적색목록에 속하는 관속식물은 취약종(VU) 갯취 등 총 8분류군이다.

This study aimed to estimate the effect of inoculant application level on chemical composition and bacterial count of fermented chestnut meal (FCM), and its rumen fermentation characteristics. The inoculant contained Lactobacillus acidophilus (1.2 x 1010 cfu/g), Bacillus subtilis (2.1 x 1010 cfu/g), and Saccharomyces cerevisiae (2.3 x 1010 cfu/g). The chestnut meal mixed with molasses, double distilled water, and inoculant at 1 kg, 3 g, 480 mL, and 20 mL ratio for the basal chestnut meal diet. The double distilled water from basal chestnut meal diet was substituted with bacterial inoculant at a level of 0 (Control), 20 (Medium), and 40 mL (High) in the experimental diets. The mixed experimental diets were incubated at 39°C for 7, 14, and 21 days, respectively. On 7 days of FCM incubation, the contents of crude protein (CP) (quadratic, P=0.043) and neutral detergent fiber (quadratic, P=0.071) decreased by increases of inoculant application levels, whereas bacterial count (quadratic, P=0.065) and rumen NH3-N (linear, P=0.063) increased. By increases of inoculant application levels on 14 days of FCM incubation, the increases were found on dry matter (DM) (quadratic, P=0.085), CP (quadratic, P=0.059), acid detergent fiber (quadratic, P=0.056), in vitro DM digestibility (linear, P=0.002), rumen total volatile fatty acid (VFA) (linear, P=0.057), and rumen iso-butyrate (linear, P=0.054). However, the decreases were found on bacterial count (linear, P=0.002), propionate (linear, P=0.099), and butyrate (quadratic, P=0.082). On 21 days of FCM incubation, in vitro DM digestibility (linear, P=0.002) and total VFA (linear, P=0.001) increased by increases of inoculant application levels, whereas the contents of CP (quadratic, P=0.034) and neutral detergent fiber (quadratic, P=0.047) decreased. These results indicate that the FCM with a medium level of inoculant application and 14 of fermentation had beneficial effects by increasing DM digestibility and rumen total VFA content, without altering bacterial count.

In this study, the effect of probiotic supplementation on growth performance, blood metabolites, and meat quality of Hanwoo steer was investigated. A total of 32 Hanwoo steers (15-17 months, average body weight 462±37.9 kg) were randomly allotted to 4 dietary treatments (0, 0.5, 1.0, and 1.5% mixed probiotics), with four Hanwoo steers per pen (two replicates per treatments), and reared for 12 months. There were no differences among treatments in growth performance of Hanwoo steer (P>0.05); however, feed intake decreased linearly with increasing levels of mixed probiotics. Growth hormone and Blood Urea Nitrogen (BUN) levels responded linearly with increasing levels of dietary mixed probiotics (P<0.05), but not insulin and blood glucose did not. In particular, total cholesterol was significantly lower for the 1% mixed probiotic treatment in comparison with that of the other treatments (P<0.05). The pH, Thiobarbituric Acid Reactive Substances (TBARS), cooking loss, and meat color were influenced by increasing levels of mixed probiotics (P<0.05), but the carcass characteristics and shear force were not. Regarding sensory evaluation, the addition of mixed probiotics resulted in significant difference in meat color, tenderness, aroma, off-flavor, juiciness, and marbling score, but not in overall acceptability. In addition, fatty acid profiles indicated no differences between control and mixed probiotic treatments. In conclusion, mixed probiotic treatment at 1% levels can enhance consumer preferences possibly by reducing cholesterol and TBARS.

This study was carried out to investigate the vascular plants of Mt. Nochu (1,322m) from April, 2014 to October, 2015. The vascular plants were summarized as 515 taxa, including 89 families, 304 genera, 448 species, 4 subspecies, 57 varieties and 6 forms. Among the investigated 515 taxa, 11 Korean endemic plants and 14 rare and endangered plants were included. The floristic regional indicator plants found in this area were 11 taxa of grade IV, 30 taxa of grade III. Naturalized plants consisted of 24 taxa that made up 4.6% of the total vascular plants in this area. Medicinal plants of the Korean Pharmacopoeia and Korean Herbal Pharmacopoeia distributed in this area consisted of 67 taxa.

The objectives of this study were to evaluate the effect of replacing 40% corn meal with three different types of barley (Youngyang, Wooho, and Yuyeon) on the chemical compositions, microbial indices, gas emission, and volatile fatty acid (VFA) content in feces of growing pigs. Sixteen pigs (Landrace × Yorkshire × Duroc) with an initial average body weight of 71 ± 2 kg were maintained in metabolic cages and randomly allotted to four treatments containing different sources of barley, for 23 days. The treatment with three different barley types replacing 40% ground corn showed no effect (P>0.05) on fecal chemical compositions, microbial indices, gas emission, and VFA. However, some differences (P<0.05) were noted in the chemical compositions of crude fiber and ash, and in the levels of Lactobacillus and Salmonella in the feces of the growing pigs. In conclusion, methane and hydrogen sulfide decreased by replacing 40% corn meal with Youngyang and Wooho barley, respectively.

여드름은 면포, 구진, 낭종, 결절, 색소 침착 등 다양한 피부병변으로 나타나는 모낭피지선의 만성 염증질 환으로 사춘기부터 성인기까지 발생 연령대가 다양해지고 있다. 한편, 약물 부작용으로 여드름이 발생하기도 하 는데, 표피성장인자(epidermal grouwth factor, EGF) 수용체 억제제 항암제를 사용할 경우 75 ∼ 100%에서 여드름양 모낭염이 발생된다고 보고되고 있다. 여드름의 치료로 항생제, 레티노이드 경구 복용 및 외용 약제 도 포 등 다양한 방법이 이용되고 있다. 그러나 최근 들어 레티노이드 기형 유발 가능성 및 Propionibacterium acne의 항생제 내성률 증가는 기존 치료의 한계로 여겨진다. 따라서 본 연구는 최근 여드름양 발진에 효과가 있다고 알려진 EGF를 함유한 외용제가 여드름 치료에 미치는 효과와 안정성을 평가하였다. 한국 성인 10 ∼ 29세 23명을 대상으로 EGF 함유 제품(트러블컨트롤 EGF)과 3종 제품(트러블컨트롤 클래리파잉 클렌징폼, 트 러블컨트롤 올-클리어 필링토너, 레드롤 카밍 모이스처)을 하루 두 번 사용하도록 하였다. 사용 후 영상 피지량, 경표피수분손실량, 피부 홍조 측정, 전문가 육안 평가, 사용 후 만족도 설문조사를 평가하였다. 최종 측정 시, 피부 피지량, 경피수분손실량, 피부 홍조가 통계학적으로 감소하였으며, 전문가 육안 평가에서 여드름 병변(면 포, 구진)도 통계학적으로 감소하였다. 연구동안 심각한 부작용은 관찰되지 않았다. 표피성장인자 함유 외용제는 경도의 여드름에 안전하면서도 효과적으로 사용할 수 있을 것으로 생각된다.

To realize a robot hand interacting like a human hand, there are many tactile sensors sensing normal force, shear force, torque, shape, roughness and temperature. This sensing signal is essential to manipulate object accurately with robot hand. In particular, slip sensors make manipulation more accurate and breakless to object. Up to now several slip sensors were developed and applied to robot hand. Many of them used complicate algorithm and signal processing with vibration data. In this paper, we developed novel principle slip sensor using separation layer. These two layers are moved from each other when slip occur. Developed sensor can sense slip signal by measuring this relative displacement between two layers. Also our principle makes slip signal decoupled from normal force and shear force without other sensors. The sensor was fabricated using the NBR(acrylo-nitrile butadiene rubber) and the Ecoflex as substrate and a paper as dielectric. To verify our sensor, slip experiment and normal force decoupling test were conducted.

이 연구의 목적은 고등학생의 물리와 생물에 관한 관점을 비교하고, 성별, 과학 성적, 수능 과학 선택 과목이 물리와 생물에 관한 관점 차이에 미치는 영향을 알아보는 것이다. 228명의 자연과정 고등학생을 대상으로 검사지 VASS를 사용하여 물리와 생물에 관한 관점을 조사하 였다. 학생의 과학의 본성에 대한 이해는 과학 영역에 따라 다르게 나타나 영역 특이적인 성향 을 보였다. 즉, 학생들이 물리 지식 및 학습에 대한 인식은 생물 지식 및 학습에 대한 인식과 차이를 보였다. 이와 같은 물리와 생물에 관한 관점 차이는 과학 성적, 성별, 물리 선택, 생물 선택에 따라 영향을 받는 것으로 나타났다. 과학 성적이 높을수록, 여자보다는 남자가, 수능에 서 물리를 선택한 경우가 물리에 관한 관점이 생물에 관한 관점보다 높았으며, 수능에서 생물 을 선택한 경우는 생물에 관한 관점이 물리에 관한 관점보다 높았다.