Following the social requirement to strengthen field supervision of the asbestos containing materials (ACM) abatement process with regard to asbestos school buildings, this study was conducted to understand the status and characteristics of airborne asbestos that may potentially occur after the ACM abatement process is completed. In the area where a series of asbestos abatement processes were finally completed, comprehensive area air sampling was performed. For sample analysis, Transmission Electron Microscopy (TEM) was used according to The Asbestos Hazard Emergency Response Act (AHERA) method and Phase Contrast Microscopy (PCM) analysis was also performed. Airborne asbestos was detected in 29.5% of the total samples, and the average concentration was 0.0039 ± 0.0123 s/cc (12.3 ± 38.9 s/mm2). 4.5% of the total samples exceeded the AHERA standard (70.0 s/mm2) and the average concentration was 0.0528 ± 0.0256 s/cc (167.2 ± 82.0 s/mm2). Airborne asbestos was no longer detected at the point when AHERA is exceeded after re-cleaning. Most of the detected asbestos was chrysotile (94.4%) and the structure types of asbestos were Matrix (41.4%), Fiber (39.9%), Bundle (10.8%), and Cluster (7.8%). Among the asbestos structures detected through transmission electron microscope analysis, the asbestos structures satisfying PCM-equivalent structures were found to be 6% of the detected asbestos, indicating that there is a limitation of the PCM analysis to check the airborne asbestos in that area. As a result of reviewing the status of airborne asbestos that may potentially occur and the type and dimensions of asbestos structure detected in the area, since the airborne asbestos exposure caused by poor field supervision for the ACM abatement process could not be ruled out, thorough management is necessary. In addition, the result of this study could be used as scientific evidence for establishing and strengthening policies related to ACM abatement, including cases of school buildings.

본 연구의 목적은 요가와 자매 학문으로서 삶의과학, 예방의학, 통합의학, 체질의학이라고 할 수 있는 아유르베다를 기반으로, 백세 시대의 건강을 목표로 미각을 중시하는 식생활교육 현장에서의 접 목법을 찾고자 했다. 연구방법으로는 아유르베다의 몸 이해를 위해 첫째, 독특한 개념인 3-도샤(바따, 피타, 카파)와 기능, 구조, 질료를 포함하는 개념인 7-다뚜(dhātu), 질료의 개념인 3-말라를 살펴보았 다. 둘째, 아유르베다의 6가지 맛(단맛, 신맛, 쓴맛, 짠맛, 매운맛, 떫은맛)의 의미와 현대 음식의 맛에 대해 문헌고찰. 셋째, 오감 중에 음식의 맛과 관련이 깊은 미각력을 중심으로 문헌고찰하였다. 또한, 미각교육 시 적용이 쉬운 간단 미각테스트 활용법과 함께 일상에서 현대인의 미각력을 높이는 특이 한 음식조합을 제시하였다. 결론에서는 아유르베다의 맛과 현대 음식의 맛 의미를 정리하면서 백세 시대를 사는 현대인들에게 아유르베다적 식생활법을 권장하고, 내 몸에 맞는 맛을 찾아가며 보다 건 강한 삶을 영위할 것을 제언했다.

한국의 전통 과자인 유과는 거의 모든 공정이 수작업으로 제조되기 때문에 공정 중 작업자 및 작업도구 등에 의한 교차오염으로 인한 안전문제가 제기되고 있다. 본 연구에서는 유과 제조 공정 중 찹쌀의 불림 단계에서 증가된 미생물을 제어하기 위하여 항균 활성이 있는 용액 9가지 용액(40% 자몽종자 추출물, 100% 계피 추출물, 70% 에탄올, 100% 식초, 0.2% 차아염소산나트륨, 40% 초산나트륨, 40% 탄산수소나트륨, 40% 사과산, 40% 구연산)을 이용하여 Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Salmonella typhimurium, Bacillus cereus에 대해 항균 효과를 비교 확인하였다. Disk diffusion법을 통해 40% 사과산과 구연산에서 높은 항균 효과를 확인할 수 있었고, 추가로 항균용액의 농도, 처리방법 및 시간을 최적화한 결과 1% 사과산-구연산을 10분 동안 처리 시 세균 5종을 모두 사멸시켜 높은 항균효과를 확인할 수 있었다. 불림단계의 찹쌀에 1% 사과산-구연산으로 정치 및 교반하면서 10분 동안 세척하였을 때 일반세균과 대장균군의 수가 각각 4.0 Log CFU/g와 5.0 Log CFU/g씩 감소하였다. 이상의 결과로 볼 때 1% 사과산-구연산으로 불린 찹쌀에 대해 침지와 교반으로 10분 동안 처리함으로써 전통 유과 제조과정 중 쌀의 불림단계에서 증가된 미생물을 효과적으로 저감화 할 수 있음을 확인하였다.

세리신은 누에고치에서 추출한 단백질로 많은 건강상의 이점을 가지고 있다. 본 연구는 화장품 소재로서 누에고치에서 유래된 세리신 표준품의 항주름 활성 및 항염증 활성을 평가하기 위해 수행되었다. 세리신의 항산화 효과는 DPPH 및 ABTS 측정법에 의해 측정되었다. 또한 대식세포인 Raw 264.7 cell에서 의 세포 생존율을 확인하였으며, lipoplyscaccharide를 이용하여 유도된 염증반응을 이용하여 세리신의 항 염증 효과를 조사하였다. 그 결과 세리신은 DPPH, ABTS에서 항산화 활성을 보였으며 세포 독성을 가지지 않은 1,000 μg/mL의 농도에서 NO를 억제하였다. 종합하여 세리신은 항산화 활성을 가지며 항노화 및 항 염증 화장품의 우수한 소재가 될 수 있음을 보여준다.

To investigate the anti-inflammatory activity of submerged culture using Cordyceps militaris mycelium, culture-including mycelia was extracted and lyophilized into postbiotics (hot-water extract; CM-HW). HW was fractionated into crude polysaccharide (CM-CP) by ethanol precipitation, and CM-CP was further dialyzed into CM-DCP by dialysis with running water using 12~14 kDa dialysis tube. When the cytotoxicity of subfractions against cells was assessed, no subfraction had a cytotoxic impact that was substantially different from the control groups. In an inflammatory model using LPS-stimulated RAW 264.7 cells, CM-DCP significantly decreased IL-6 and MCP-1 production levels compared to the LPS-control group. CM-DCP also inhibited IL-6 and IL-8 secretion in HaCaT keratinocytes stimulated with TNF-α and IFN-γ. In the meanwhile, the neutral sugar content and mannose ratio of anti-inflammatory CM-DCP were higher than the other fractions, and CM-DCP contained β-1,3/1,6-glucan of 216.1 mg/g. High pressure size exclusion chromatography revealed that CM-DCP contained molecules with a molecular weight range of 5.6 to 144.0 kDa. In conclusion, postbiotics of C. militaris mycelium significantly promoted anti-inflammatory activity, suggesting that neutral polysaccharides including Glc and Man contribute to the anti-inflammation in RAW 264.7 or HaCaT cells.

본 연구에서는 사례연구 대상지역(인천)의 특화산업에 대해 LQ지수분석을 통해 해양특화산업을 도출하고, 특화산업의 해양 공간 활용현황과 용도구역 설정에 따른 현안을 진단하였다. 대상지역의 해양산업에 대한 LQ지수(Location Quotients, LQ) 분석을 통해 사 업체수 기준 LQ지수 1.08, 종사자수 기준 LQ지수 1.67로 전국평균 대비 높은 해양바이오산업을 인천지역 해양특화산업으로 선정하였 다. 해양특화산업인 해양바이오산업의 현안진단을 위해 영흥도 실증 배양장 사례 검토 및 가장 낮은 특화도를 나타낸 해양에너지개발 부문에서의 현안 진단을 통해 해양용도구역의 중첩문제, 이해관계자간 민원발생 이슈 등 현장문제 해결을 위해서 객관적이고 정량적 근거를 기반으로 정책의사결정지원시스템 마련이 필요하다는 결론을 제시하였다.

보호지역의 생태적 가치와 중요성에 대한 인식이 증가함에 따라 자연이 제공하는 생태계서비스에 관한 연구의 필요 성이 요구되고 있다. 우리나라의 대표적인 보호지역인 자연공원은 국립공원, 도립공원, 군립공원의 체계를 가지고 있다. 국립공원은 국립공원공단에서 체계적으로 관리를 하고 있으나 도립공원과 군립공원은 지방자체단체가 관리하고 있다. 자연공원의 위계에 따라 자연성 또한 같은 위계적 차이가 있을 것으로 생각되나 이에 대한 검증은 이뤄지지 않았다. 이에 대한 차이를 알아보기 위하여 자연공원 중 산악형 22개소를 대상으로 InVEST 모델 중 서식지질(habitat quality)을 이용하여 차이를 알아보았다. 자연공원의 종류와 면적에 관계없이 서식지질을 분석한 결과 태백산국립공원 (0.89), 주왕산국립공원(0.87), 웅석봉군립공원(0.86), 가야산국립공원(0.85) 순으로 높게 나타났다. 서식지질은 면적인 넓을수록 높은 값을 나타내었다. 면적이 유사한 자연공원을 대상으로 분석한 결과 국립공원의 서식지질은 도립공원과 군립공원에 비해 높았다. 반면 군립공원의 서식지질 평균은 0.83±0.02으로 도립공원의 서식지질 평균 0.78±0.03보다 0.05 더 높게 나타났다. 아울러 자연공원내에서 산림지역 비율이 높을수록 서식지질 또한 비례하여 높게 나타났다. 이상의 결과를 종합하면, 자연공원의 자연성은 자연공원의 위계와 같지 않고 토지이용, 토지피복 그리고 공원관리 등에 따라 자연성에 차이가 있음을 확인하였다.

The Cheonggukjang used in this study was made with four soybean cultivars grown and harvested at the National Agrobiodiversity Center of the National Academy of Agricultural Sciences: C1 (KLS87248), C2 (Nongrim 51), C3 (GNU- 2007-14613), and C4 (Daewon). The soybeans were inoculated with Bacillus subtilis AFY-2 and fermented for 0, 12, 21, 36, 45, and 60 h in a culture room at 35°C. ABTS and DPPH radical scavenging activities were significantly increased upon fermentation for 12 hours in all samples and maintained or decreased after 21 hour The inhibition of NO production was significantly increased compared to the LPS-treated group, and the C2 sample showed inhibition of NO production at 12 hours of fermentation, and the C3 sample showed inhibition at 21 hours of fermentation. PCA analysis revealed that the ABTS and DPPH radical scavenging activity and NO production inhibitory activity reached peak levels around 12 h fermentation time for all samples. Our results indicate that the optimal fermentation time of the Cheonggukjang sample is 12 hours, confirming the high correlation of all experiments.

Barnea manilensis is a bivalve which bores soft rocks, such as, limestone or mudstone in the low intertidal zone. They make burrows which have narrow entrances and wide interiors and live in these burrows for a lifetime. In this study, the morphology and the microstructure of the valve of rock-boring clam B. manilensis were observed using a stereoscopic microscope and FE-SEM, respectively. The chemical composition of specific part of the valve was assessed by energy dispersive X-ray spectroscopy (EDS) analysis. 3D modeling and structural dynamic analysis were used to simulate the boring behavior of B. manilensis. Microscopy results showed that the valve was asymmetric with plow-like spikes which were located on the anterior surface of the valve and were distributed in a specific direction. The anterior parts of the valve were thicker than the posterior parts. EDS results indicated that the valve mainly consisted of calcium carbonate, while metal elements, such as, Al, Si, Mn, Fe, and Mg were detected on the outer surface of the anterior spikes. It was assumed that the metal elements increased the strength of the valve, thus helping the B. manilensis to bore sediment. The simulation showed that spikes located on the anterior part of the valve received a load at all angles. It was suggested that the anterior part of the shell received the load while drilling rocks. The boring mechanism using the amorphous valve of B. manilensis is expected to be used as basic data to devise an efficient drilling mechanism.

To enhance the bioavailability and bioactivities of mixed herbal medicines (RW), they were fermented with lactic-acid bacteria isolated from kimchi into postbiotics (FRW). Then, from the results of the 16s rRNA sequencing analysis, lactic acid bacteria isolated from kimchi were identified to be of two species, namely Lactobacillus sakei and Leuconostoc mesenteroides. The FRW prepared from the RW were extracted using hot water (HW) and 70% EtOH (EtOH) for comparison of their macrophage-stimulating activities. Based on a comparison of the activities of the FRW extracts, nitric oxide (NO) production of HW was significantly higher than that in EtOH. An analysis of the chemical properties of the extracts showed that HW had higher contents of neutral sugar and uronic acid than EtOH as well as contained a large amount of glucose. In addition, crude polysaccharide (CP) was prepared to enhance the macrophage-stimulating activity. The FRW-CP not only secreted immunostimulatory mediators but also increased the expression of immunostimulatory genes (iNOS, TNF-α, MCP-1, and IL-6). The fractionated FRW-CP contained about 90% neutral sugars, and these sugars were mainly composed of glucose, galacturonic acid, and arabinose. Thus, FRW prepared by fermentation of RW with kimchi lactic acid bacteria were found to be immunostimulatory modulators.

Although monitoring of radon has been extensively implemented throughout South Korea, the risk assessment has been mainly limited to indoor environments such as schools, workplaces, and multi-use facilities, and evaluations have normally been performed separately. In this study, the differences in radon exposure according to two groups (< 1 and 1-6 years old) were evaluated considering various indoor and outdoor environments, timeactivity patterns, variations in radon concentrations, and dwelling type (single detached and apartment house) using Monte-Carlo simulation. The distribution and representative values of radon concentration by micro-environments were confirmed through the Anders-Darling test, and a uniform distribution was applied in case of uncertainty. The effective dose ranged from 1.81 ± 1.19 to 2.81 ± 3.02mSv/y. Comparing the levels recommended by EPA, WHO, and ICRP with the value of the 95th percentile of this study, it was found that the results for those dwelling in detached houses exceeded recommended levels. Infants that spend a lot of time in homes with relatively high levels of concentration of radon are assessed to be somewhat more vulnerable to radon exposure.

This study analyzes the effects of the number of angles and bends on resistance in a conductor-embroidered stitch circuit for efficient power transfer through a conductor of wearable energy harvesting to study changes in power lost through connection with actual solar panels. In this study, the angle of the conductive stitch circuit was designed in units of 30°, from 30° to 180°, and the resistance was measured using an analog Discovery 2 device. The measured resistance value was analyzed, and in the section of the angle where the resistance value rapidly changes, it was measured again and analyzed in units of 5°. Following this, from the results of the analysis, the angle at which the tension was applied to the stitch converges was analyzed, and the resistance was measured again by varying the number of bends of the stitch at the given angle. The resistance decreases as the angle of the stitch decreases and the number of bends increases, and the conductor embroidery stitch can reduce the loss of power by 1.61 times relative to general embroidery. These results suggest that the stitching of embroidery has a significant effect on the power transfer in the transmission through the conductors of wearable energy harvesting. These results indicate the need for a follow-up study to develop a conductor circuit design technology that compares and analyzes various types of stitches, such as curved stitches, and the number of conductors, so that wearable energy harvesting can be more efficiently produced and stored.

After liquid culture of Phellinus baumii (P. baumii) mycelium (LPBM) was prepared, LPBM was fractionated into A∼E fraction (A; hot-water extract of liquid culture including mycelia, B; crude polysaccharide of A, C; hot-water extract of mycelia, D; crude polysaccharide of C, and E; crude polysaccharide of culture broth) to evaluate for possibility as functional materials with immunostimulatory activity. In macrophage stimulatory activity, E fraction as postbiotics significantly increased secretion of NO and IL-12 from RAW 264.7 cells. Next, when the splenocytes of C3H/HeN mice were primary cultured, E fraction showed significantly mitogenic activity with enhancing mitogen-related cytokines (IFN-γ and TNF-α) production from splenocyte. E fraction also potently stimulated GM-CSF production from Peyer’s patch cells as well as Peyer’s patch-mediated bone marrow cell proliferation. In addition, the immunostimularoy E fraction contained neutral sugar (73.8%), uronic acid (10.6%), protein (7.8%), and polyphenol (7.5%), and mainly consisted of glucose (39.1%), galactose (21.7%), mannose (11.1%), galacturonic acid (9.9%), and arabinose (8.9%) as component sugars. In conclusion, it was demonstrated that postbiotics including exopolysaccharide fractionated from liquid culture of the P. baumii mycelium could enhanced immunostimulatory activity.

In this study, probiotic properties such as acid resistance, bile tolerance, intestinal adhesion and fat accumulation inhibition of lactic acid bacteria isolated from kimchi were investigated in order to develop probiotics effective anti-adipogenic effects. Among the 120 types of lactic acid bacteria isolated from kimchi, 42 types of lactic acid bacteria being to acid resistance, 35 types of lactic acid bacteria being to bile resistance, and 24 types of lactic acid bacteria being to both acid resistance and bile resistance were selected. These 24 types were tested for the intestinal adhesion ability which is an essential factor of probiotics’ function and L. rhamnosus GG was used as a positive control in this test. 10 types of lactic acid bacteria having the intestinal adhesion ability were selected. The selected 10 types of lactic acid bacteria were treated in 3T3-L1 cells and intracellular fat was stained with Oil Red O reagent to measure fat accumulation. As a result, in the group treated with lactic acid bacteria OS-15, 48, 102 and 120, fat accumulation was significantly decreased compared with the non-treated group. And the strain OS-48 showed the greatest inhibitory effect on fat accumulation. The lactic acid bacterium OS-48 was identified as Lactiplantibacillus plantarum by analysis based on 16S rRNA sequencing and was named Lactiplantibacillus plantarum OS-48. Therefore, based on the results of this study, L. plantarum OS-48 is expected to be very useful as probiotics with anti-obesity effect in the food industry such as fermented food and functional food.

이미지 인식에 특화된 CNN (Convolutional Neural Network) 기반의 딥러닝 기법은 영상의 항목별 분류가 필요한 다양한 연구에 적용되고 있다. 본 연구는 건물, 도로, 논, 밭, 산림, 나지의 6가지 항목을 산림복원 대상 후보지로 정의하고 CNN 기반의 산림복원 대상 후보지 추출 및 분류의 최적 방법론을 탐색하였다. 6,640개의 데이터셋을 75:25의 비율로 훈련(4,980개) 및 검증(1,660개)로 구분하여 구축하고 학습에 활용하였다. 모델별 정확도는 픽셀정확도(PA), 평균 교차 겹침 결합(Mean IoU)을 이용하여 평가하였다. 픽셀정확도는 90.6%, 평균 교차 겹침 결합은 80.8%로 산정되어 Inception-Resnet-v2 모델이 세 모델 중 가장 산림복원 대상 후보지 추출에 뛰어난 정확도를 보였다. 이 결과는 기존의 산림복원 대상 후보지 현장조사 혹은 항공사진을 활용한 조사에 비해 시공간적 이점을 가지며, 향후 산림복원 대상지 선정 자료로 적용 가능성이 있다고 판단된다.

목적 : 본 연구는 미국의 조건부 운전면허 관련 상세 운영 현황을 알아보고, 분석한 결과를 바탕으로 고령운전자 안전운전 을 위한 국내 조건부 운전면허 도입 시 참고가 될 수 있는 기초자료를 마련하고자 하였다. 또한, 운전재활전문가로서 작 업치료사의 조건부 운전면허에 대한 관심을 제고시키고자 하였다. 연구방법 : 미국자동차협회 교통안전재단(AAA Foundation for Traffic Safety)의 자료를 참고하여 미국의 고령운전자 사고율이 25% 이상에 해당하는 상위 4개 주인 켄터키, 미시시피, 몬태나, 와이오밍 주의 고령운전자 조건부 운전면허 관 련 현황을 정리하였다. 결과 : 본 연구에서는 미국 4개 주의 고령운전자의 운전면허 갱신, 고령운전자 조건부 운전면허 실시 및 제한 방법, 고령 운전자 조건부 운전면허 결정 판단 주체, 지역 현지 심사관(Local examiner)의 고령운전자 조건부 운전면허 결정과 관 련된 현황을 주로 파악해보았다. 조건부 운전면허 제한 유형으로는 고속도로 접근 제한과 거리 제한, 차량 장비 등이 있 었고 각 주의 공통적인 부분으로는 주간이나 낮에만 운전할 수 있도록 야간운전 제한 및 속도를 제한하고 있음을 파악하 였다. 결론 : 본 연구는 미국 4개 주의 고령운전자 조건부 운전면허제도 관련 현황 분석을 통해 우리나라 고령운전자 조건부 운 전면허제도 도입 시 참고 가능한 자료임에 의의가 있다. 앞으로 우리나라 고령운전자를 위한 국내 환경에 맞는 조건부 운전면허제도 도입과 관련된 기초자료로 활용될 수 있을 것으로 사료된다.