Background : It is known that Platycodon grandiflorum has anti-inflammatory activity and inhibits the production of nitric oxide (NO) in inflammatory macrophages. But the change of bioactivity of platycodon grandiflorum according to steaming is not well known. In this study, We investigated the effects of steaming on anti-inflammatory activity of 70% ethanol extracts of platycodon grandiflorum. Methods and Result : The cytotoxicity of RAW264.7 cells treated with platycodon grandiflorum extracts was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the concentration of NO in culture supernatants were determined using nitric oxide (NO) assay. And western blotting was performed to quantify the expression of iNOS, a protein related to NO production. As a results, it was confirmed that no cytotoxicity was observed at 25, 50 and 100 ㎍/㎖ platycodon grandiflorum extracts in RAW264.7 cells. The production of NO and the expression of iNOS were induced by LPS and suppressed by all platycodon grandiflorum extracts in proportion to the number of steaming in RAW264.7 cells. Conclusion : These results suggest that a steaming process can increase anti-inflammatory activity of platycodon grandiflorum extracts.

본 연구에서는 그라비올라(Annona muricata) 잎으로부터 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획을 제조하였고 이들 추출물/분획에 대하여 항산화 활성을 평가하였다. 1,1-Phenyl-2-picrylhydrazyl (DPPH) 라디칼 시험법을 이용한 자유라디칼 소거 활성, 루미놀 발광법을 이용한 총 항산화능 및 1O2 소광 효과를 평가하였다. 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획의 라디칼 소거 활성 (FSC50)은 각각 45.6, 29.8 및 18.0 μ g/mL이었고, 총 항산화능(OSC50)은 4.4, 1.1 및 2.8 μ g/mL이었다. 에틸 아세테이트 분획의 총 항산화능은 수용성 항산화제로 잘 알려진 L-ascorbic acid (1.5 μ g/mL)보다 높은 항산 화능을 나타내었다. 1O2 소광 상수 실험 결과, 에틸아세테이트 및 아글리콘 분획은 비교물질로 사용된 L-ascorbic acid와 유사한 활성을 보여주었다. 1O2으로 유도된 적혈구 세포 손상에 있어서, 그라비올라 잎 50% 에탄 올 추출물은 농도 의존적(5-50 μ g/mL)으로 세포보호 활성을 나타내었다. 실험에 사용된 그라비올라 잎 추출 물의 에틸아세테이트 분획과 아글리콘 분획에 대하여 TLC 및 HPLC를 이용한 성분 분석을 수행하였다. 에틸아 세테이트 분획에서는 rutin (quercetin-3-O-rutinoside), kaempferol-3-O-neohesperidoside, nicotiflorin (kaempferol-3-O-rutinoside), p-coumaric acid을 확인하였다. 아글리콘 분획에서는 kaempferol이 존재함을 확인하였다. 이상의 결과들은 그라비올라 잎 추출물이 항산화 화장품 원료로서 응용 가능성이 있음을 시사한다.

As a result of inquiring into the analysis consequent on a symptom by research subject, it was found that there were 1 paper (1.5%) which did research for the purpose of rehabilitation of the general public, 26 papers (38.8%) targeting diagnosis-related groups (DRG), and 40 papers (59.7%) targeting social consideration subject. Also, as a result of the inquiry about the frequency & number of times of horticultural therapy program implementation, it was found that 49 papers (73.1%) implemented one time a week, and 45 papers (67.2%) were surveyed as the highest by conducting a total of 11~20 sessions of horticultural therapy programs, In the analysis of horticultural therapy activities by type, plant cultivation activity was found to be the most 506 times, accounting for 41.5%, followed by 297 time crafts activity (24.4%), 213 time floral decoration activity (17.5%), and 203time others activities (16.7%). In cultivation activity, soil-using cultivation activity (25.1%) was found to be lower than the proportion (74.9%) of soiless cultivation (16.4%), crafts activity (24.4%), floral decoration activity (17.5%) and other activities (16.7%). The most used plants in a restricted place like a hospital were found to be in the order of Hedera helix, Chamaedorea elegans, Succulent plant, Syngonium podophyllum, Neofinetia falcata HU, Hoya carnosa (L.f.) R.Br.), Rosmarinus officinalis, and Spathiphyllum spp.

Background: This study was performed to evaluate the protective effect of Saururus chinensis ethanol extract (SCE) against styrene toxicity in mouse spermatocyte cells [GC-2spd (ts) cell line]. Methods and Results: Cytotoxicity in mouse spermatocyte cells was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Generation of reactive oxygen species (ROS) was determined using 2’,7’-dichlorodihydrofluorescein diacetate (DCF-DA) assay. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and western blotting were performed to quantify the mRNA and protein expression levels, resepectiviely, of stress or apoptosis-related genes including p21, p53, heat shock protein 70 (Hsp70), heat shock protein 90 (Hsp90), Bax, Bcl-2, and caspase-3. The results of the MTT assay showed that 50 ㎍/㎖ SCE did not affect cell viability. ROS generation in mouse spermatocyte cells increased by treatment with 100 μM styrene, and decreased by co-treatment with SCE. SCE repressed the mRNA expression of stress-related genes, which increased by styrene treatment. In addition, SCE inhibited the apoptosis of mouse spermatocyte cells by ameliorating mRNA and protein levels of apoptotic genes that were altered by styrene treatment. Conclusions: These results suggest that SCE may alleviate styrene toxicity in mouse spermatocyte cells by reducing ROS stress and regulating genes related to styrene toxicity.

Background : Acanthopanax sessiliflorus (Rupr. et Maxim) Seem, belonging to the Araliaceae family, is widely distributed in Korea, China, and Japan. The plants belonging to Acanthopanax species are traditionally used in Korea as anti-rheumatoid arthritis, anti-inflammatory and anti-diabetic drugs and are recognized to have ginseng-like activities. A simple and sensitive high-performance liquid chromatographic (HPLC) method was developed and validated for independent analysis of major compounds and chlorogenic acid in A. sessiliflorus fruits. Chlorogenic acid was reported that prevent cancer and cardiovascular disease in vivo. Also, it has antioxidant effect in vitro test. In the previous experiment, chlorogenic acid were found in A. sessiliflorus fruits. This study was performed to identification of the major compounds and investigate the method validation for the determination of chlorogenic acid in A. sessiliflorus fruits. Methods and Results : Three major compounds were recorded on a Varian Unity Inova AS-400 FT-NMR spectrometer and analyzed by the new HPLC analysis method. HPLC analysis was carried out using an Waters e2695 and PDA detector. The new analyasis method was validated by the measurement of intra-day, inter-day precision, accuracy, limit of detection (LOD, S/N=3), and limit of quantification (LOQ, S/N=10) of chlorogenic acid. The results showed that the correlation coefficient (R2) for the calibration curves of chlorogenic acid was 0.997 in terms of linearity. The limit of detection (LOD) and limit of quantification (LOQ) were 0.565 ㎍/ml and 2.88 ㎍/ml, respectively. There was no interfering peak observed each other and HPLC system was suitable for analysis showing goodness of peak and high precision. Conclusion : This method is suitable to detect and quantify major compounds in A. sessiliflorus fruits. Furthermore, the result will be applied to establish chlorogenic acid as an standard compound for A. sessiliflorus fruits.

Background : Allergy is a common disease caused by type I hypersensitivity reaction of the immune system. Unfortunately, there is no proper treatment for allergy. Therefore, the discovery of therapeutic drugs for allergy is essential. In this study, the crude extracts of 56 plant parts were screened for anti-allergy effects in RBL-2H3 cells. Methods and Results : IgE-sensitized RBL-2H3 cells were individually treated with 56 extracts of medicinal herbs at the final concentration of 20 ㎍/㎖ and stimulated with the antigen DNP-BSA. β-Hexosaminidase release, interleukin-4 (IL-4) secretion, and cell viability in the sample treated cells were measured. Among the tested samples, extracts from the root of Polygonatum stenophyllum Maxim., aerial part of Acer triflorum Kom., and leaf of Pyrus pyrifolia var. culta (Makino) Nakai showed inhibitory effects on β-hexosaminidase release. The aerial part of Peucedanum japonicum Thunberg and seed of Panax ginseng C.A.Mey. showed suppressive activities on IL-4 secretion. All of the extracts were not cytotoxic at the tested concentration. Conclusion : From the result, six extracts including Polygonatum stenophyllum Maxim. (root) and Acer triflorum Kom. (aerial part) inhibited both β-hexosaminidase release and IL-4 secretion in IgE-sensitized RBL-2H3 cells. The use of these extracts for developing anti-allergy materials is suggested.

Background : Five medicinal herbs have been selected from the preliminary screening for in vitro anti-allergy activity (in RBL-2H3 cells). The present study is conducted to investigate the inhibitory effects of the medicinal herbs on allergic inflammation in other kind of cells. Methods and Results : Cells treated with five extracts prepared from Betula costata Trautv. (aerial part), Camellia sinensis L. (aerial part), Polygonatum stenophyllum Maxim. (root), Pyrus pyrifolia var. culta (Makino) Nakai (leaf), and Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. (root) were measured for mRNA levels of TNF-α on HaCaT keratinocytes stimulated by TNF-α /INF-γ and for mRNA levels of IL-2 in Jurkat T cells mediated by PMA/A23187. Pre-treatment with the five extracts reduced the mRNA levels of TNF-α in HaCaT cells and mRNA levels of IL-2 in Jurkat T cells. In particular, the leaf extract of Pyrus pyrifolia var. culta (Makino) Nakai significantly and dose-dependently decreased the mRNA levels of TNF-α and IL-2. To determine the toxicity of the extracts from the selected medicinal herbs to HaCaT cells and Jurkat T cells, the viabilities of the cells treated with several concentrations of the five extracts were measured by MTT assay. Extracts of Polygonatum stenophyllum Maxim. (root), Pyrus pyrifolia var. culta (Makino) Nakai (leaf), and Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. (root) (up to 250 ㎍/㎖) did not show cytotoxic effects on HaCaT cells and Jurkat T cells. On the other hand, 250 ㎍/㎖ of extracts of Betula costata Trautv. (aerial part) and Camellia sinensis L. (aerial part) reduced cell viability in both cells. Conclusion : These results demonstrate that the leaf extract of Pyrus pyrifolia var. culta (Makino) Nakai has an anti-inflammatory effect by inhibiting pro-inflammatory cytokine expression. Therefore, the leaf of Pyrus pyrifolia var. culta (Makino) Nakai can be a useful resource for the development of anti-allergy/anti-inflammatory materials.

Pulmonary angiography has been regarded as the gold standard tool for diagnosis of pulmonary embolism. However, due to its invasive nature and the development and growing availability of non-invasive diagnostic imaging tests, such as computed tomographic pulmonary angiography, the role of pulmonary angiography in diagnosis of pulmonary embolism has waned rapidly, so that pulmonary angiography has become a state-of-the-art procedure available in a decreasing number of centres. Pulmonary angiography is the definitive diagnostic test, but complications of this procedure, including puncture site prob-lems, cardiac arrhythmia, and cardiac or vessel injury have been reported. However, few cases of cardiac vein rupture during pulmonary angiography have been reported. Here we report on a case of cardiac vein rupture which occurred during pulmo-nary angiography in an 82-year-old woman who underwent pulmonary angiography.

Mungbean (Vigna radiata) is a fast-growing, warm-season legume crop that is primarily cultivated in developing countries of Asia. We constructed a draft genome sequence of mungbean to facilitate genome research into the subgenus Ceratotropis and to enable a better understanding of the evolution of leguminous species. The draft genome sequence covers 80% of the estimated genome, of which 50.1% consists of repetitive sequences. In total, 22,427 high confidence protein-coding genes were predicted. Based on the de novo assembly of additional wild mungbean species, the divergence of what was eventually domesticated and the sampled wild mungbean species appears to have predated domestication. Moreover, the de novo assembly of a tetraploid Vigna species (Vigna reflexo-pilosa var. glabra) provided genomic evidence of a recent allopolyploid event. To further study speciation, we compared de novo RNA-seq assemblies of 22 accessions of 18 Vigna species and protein sets of Glycine max and Cajanus cajan. The species tree was constructed by a Bayesian Markov chain Monte Carlo method using highly confident orthologs shared by all 24 accessions. The present assembly of V. radiata var. radiata will facilitate genome research and accelerate molecular breeding of the subgenus Ceratotropis.

This study was performed to investigate the feasibility of ion-exchange resins as soil amendment. To select resins, weinvestigate four kinds of ion exchange resins, which ions have the most adsorption efficiency of ions (N+, PO4−, K+). Inthe Cation (H+) vs Anion (OH−)=1:2 (w:w), it showed that the highest N, P, K adsorption efficiency was about 80%and reached pH 6~7 & EC 1~2dS/m after 20min. The ability to maintain ion adsorption, it was investigated that thegentle curve of the residual ion ratio of nitrate nitrogen, available phosphorus, exchangeable potassium was maintained.On the other hand, the control (Slow release fertilizer) was significantly reduced under artificial rainfall conditions. Inaddition, the results of three consecutive crop test were showed that the growth of crops decreased 41.8% than the earlygrowth in the control group, whereas decreased 8.3% in the treatment group (ion exchange resins). According to the resultsof soil analysis, it was reduced that the fertilizer ingredients (N 94%, P 74.5%, K 98.6%) in the control and reducedfertilizer ingredients (N 64%, P 60.3%, K 58.4%) in the treatment. Overall, it was estimated that the Ion-exchange resinscould be used as a soil amendment.

This study is about making artificial soil for wall greening using Spent Mushroom substrates (SMS) & Superabsorbent polymer (SAP). and developing new material for wall greening whilst monitoring the physico-chemistry & germination ability of the plant, plant growth and developmental condition of the artificial soil. It was found that the larger the particle size of the superabsorbent polymer the more absorption increased. This showed the plant’s germination rate in soil when Spent Mushroom substrates & Superabsorbent polymer (SAP) were mixed. In the Bermuda grass, the Artificial soil (SMS & SAP 0.5%) showed a 100% germination rate over a period exceeding 7 days compared to Peat-moss that showed a 94% germination rate. In the Kentucky blue grass, however, there was no difference between the Peat-moss and Artificial soil. When general perlite soil was compared with artificial soil in the chicory seed, the Artificial soil showed better results than the general perlite soil in length, leaf volume and fresh-weight. In the plant length, it showed an increase of over 105% (SAP 0.5% or more), 187% (SAP 1% or more). in the leaf volume, an increase of over 123% (SAP 0.5% or more), 145% (SAP 1% or more), in the fresh-weight an increase of over 130% (SAP 0.5% or more), 285% (SAP 1% or more). The artificial soil (SMS & SAP 1%) showed an excellent result in the plant growth and development. Thus, it was found that artificial soil using the SMS & SAP were the only suitable soil materials for wall greening hence suggesting that it could be used for the greening of a blighted area or desert and a variety of agriculture.

This study was attempted to evaluate the change of microbial community in inoculums, lag, and stationary phase using the community level physiological profiles (CLPP) base on C-substrate utilization. It was to ascertain the characterizing microbial community over time in the enrichment step of microbial fuel cells. Microbial fuel cell is a device that converts chemical energy to electricity with aid of the catalytic reaction of microorganisms using C-substrate included wastewater. Microbial fuel cells enriched by a mixture of anaerobic digestive sludge of the sewage treatment plant and livestock wastewater were used. The current after enrichment was generated about 0.84 ± 0.06 mA. Microbial community in inoculums, lag and stationary phase used amine group, phosphorylated chemical group, and carboxylic acid group (some exclusion). However, phenolic compound did not use by microorganisms in lag and stationary phase. It means that there are not the microorganisms capable of decompose the phenol in microbial fuel cell enriched by livestock wastewater. In case of substrates of amino acid and carbohydrates group, these C-substrates were only used by microorganisms in the stationary phase. It may be that electrochemically active microorganisms (EAM) which we want to know should utilize the better these C-substrates than that of lag phase. This study showed that the electrochemically active bacteria that can be distinguished by electron changes of C-substrate utilization over time could be separated.

This study is about a kind of organic waste, food waste, and was conducted by using an alternative medium for the cultivation of oyster mushrooms to examine applicability. The study investigated the cultivation characteristics for major cultivated varieties of oyster mushrooms and the growth characteristics within food waste mediums. In addition, NaCl desalinization of food waste is being pointed out as a problem when using the medium, therefore, the effects of the medium's composition were examined by using calcium carbonate. Calcium carbonate is used for desalinization of NaCl. This study investigated the use of a total of 6 cultivars of the oyster mushroom, the Suhan (PL.1), Chiak No. 5 (PL.2), Chiak No. 7 (PL.3), Samgu No. 5 (PL.4), Samgu No. 8 (PL.5), Jangan No. 5(PL.6). In terms of medium, the optimum growth of the mycelium of the oyster mushroom was observed in the PDA medium. The optimal culture conditions was pH 6.0 in Samgu No. 8 (PL. 5) and pH 5.5-6.6 in another cultivar. In terms of food waste, the mycelium’s growth were investigated and found insufficient in the bulk of cultivars. but Chiak No. 7 (PL.3) was superior to that of other cultivars relative to the mycelium’s growth. (Mycelium’s growth: Chiak No. 7 (PL.3) = Jangan No. 5 (PL.6) > Samgu No. 8 (PL.5) > Samgu No. 5 (PL.4) > Suhan (PL.1) > Chiak No. 5 (PL.2)). In the test of desalinization using Calcium carbonate, the early salt concentration was 4.3%. When treating the calcium carbonate (1 ~ 10%) in the food waste, a change of salt concentration less than 5% of the calcium carbonate could be observed. Also, when treating the calcium carbonate (1 ~ 10%, w/w) in the food waste medium, a great change of EC could not be observed either. Therefore, the addition of calcium carbonate to food waste does not affect the growth of the mushroom, thus we had to speculate about the possibility of using food waste in the cultivation of oyster mushrooms.

본 연구는 초등학교 과학교과에 기초하여 작물 재배 중심으로 개발된 초등 원예-과학 통합 프로그램이 초등학생 6학년의 과학능력, 정서조절에 미치는 영향을 알아보고자 수행되었다. 실험군은 서울에 위치한 Y초등학교 6학년 1개 반을 대상으로 2011년 4월 8일부터 6월 24일까지 매주 1회 오후 12시 50분부터 2시까지 50시간에서 1시간 10분씩 재량활동 시간을 이용하여 총 12회에 걸쳐 초등 원예-과학 프로그램을 실행하였다. 대조군은 동일 초등학교 6학년 1개 반을 대상으로 하였다. 본 연구에서 실시한 프로그램은 초등학교 과학교과의 과학탐구기능과 원예활동을 통합하여 실내･외, 개별 및 집단으로 활동할 수 있도록 개발되었다. 과학능력을 측정하기 위하여 과학탐구능력과 과학적 태도를 프로그램 실행 전, 후에 조사하였고, 정서조절은 프로그램 실행 전 후에 자가보고형으로 조사되었다. 초등 원예-과학 통합 프로그램에 의한 과학탐구능력의 사전･사후변화는 실험군에 있어 사후총점이 유의하게 증가하였고, 대조군은 유의한 변화가 나타나지 않았다. 과학적 태도는 실험군에 있어 사후 협동성 점수가 유의하게 증가하였고, 대조군에서는 끈기성에서 유의한 감소를 나타냈다. 정서조절능력은 실험군에서 자신정서조절점수가 유의하게 증가하였고 반면 대조군의 자신정서조절점수는 유의한 감소를 나타냈다.

Achyrantes japonica (AJ) has been used to treat edema and arthritis in the traditional Korean medicine. Toelucidate the anti-inflammatory and anti-nociceptive effects of ethanol extract of AJ, the carrageenan-induced pawedema using a plethysmometer and thermal hypersensitivity using the plantar test were measured. Ibuprofen was used asa control drug. Treatment with AJ (200㎎/kg p.o.) significantly reduced paw edema, compared to the carrageenan -treated rats. In the plantar test, the thermal withdrawal latency in AJ - treated group was significantly increased than thecarrageenan - treated group. The results indicate that AJ could have be the anti-inflammatory and anti-nociceptive prop-erties.