Background: Some plants have harmful effects on fungi and bacteria as well as plants. Incorporating into soil as green manures are effective in reducing population densities of soil pathogens. Methods and Results: Twenty-three species of green manure crops were cultivated after the harvest of 6-year-old ginseng and then incorporated green manure into the soil at the flowering stage. The following year, the root rot ratio of 2-year-old ginseng and soil chemical properties were investigated. Sorghum sudanense, Helianthus annuus, and Helianthus tuberosus were relatively high in dry matter production. Without incorporating green manure into soil, NO3, EC (electric conductivity) and K were decreased by 95%, 79% and 65%, respectively. When green manure was incorporated to soil, P2O5 and NO3, were reduced by 41% and 25%, respectively. The survived root ratio of 2-year-old ginseng were significantly increased by 56.2%, 47.5%, and 47.3% in Sorghum sudanense, Ricinus communis and Helianthus tuberosus, respectively, In addition, there was a significant increase in Secale cereale, Chrysanthemum morifolium, Atractylodes macrocephala, and Smallanthus sonchifolius. The survived root ratio of ginseng showed a significant positive correlation with soil pH and a negative correlation with NO3, and EC. Conclusion: Cultivation of Chrysanthemum family mainly using rhizome and root as green manure was effective for root rot disease of ginseng.

Background : The purpose of this study was to evaluate methods to high quality medicinal vegetables, thereby increasing farm income. This study investigated the effects of different green manures crop and application culture stable foliage production in Peucedanum japonicum Thunberg. Methods and Results : Method of application with green manure crop were conducted under four condition compose to control (non application), single cropping in barley, hairy vetch and mixed cropping in hairy vetch (60%) + barley (40%)culture. Sowing dates of green manure crop were October 14 in 2014. Transplanting with Jeonam Yeosu varieties in Junel 28 by growing pot seedling for 45 days in 2015. Method of application were standard application (N-P-K-Compost applied at 20-12-10-600 ㎏․10 a−1). Plants were spaced 10 ㎝ apart in rows 10 ㎝ apart with open cultivation. Conclusions : The utilization effect of nitrogen supplied by green manure crop was highest with the hairy vetch by 21.1% in single cropping and hairy vetch + barley by 29.8% in mixed cropping. Organic matter, available phosphate, K2O, CaO, and MgO of soil increased in plots with the mixed cropping hairy vetch + barley culture. Non application < barley < hairy vetch < hairy vetch + barley in mixed cropping, aerial part as a result were so many such amount of growth as number of leaf per plant, etc., green leaf yields is untreated control plot (1,876 kg․10 a−1) compared to 7% from using barley culture, hairy vetch culture 10% higher and hairy vetch + barley culture increased to 15% of the most.

Background:The study aimed to obtain data on the effects of cultivation and soil reduction of green manure crop on the quantity and quality of organically cultivated Cynanchum wilfordii Hemsley.Methods and Results:The experiment comprised four treatments: control, hairy vetch, barley, and hairy vetch + barley (3 : 2). The plant height in the hairy vetch treatment (86.3㎝) was significantly different from that in the other treatments, whereas the stem diameter leaf area, and special product analysis division (SPAD) value did not differ across the treatments. The largest soil reduction of green manure crop was recorded in the barley treatment (440 ㎏/10 a), whereas the smallest was recorded in the single treatment with hairy vetch (80 ㎏/10 a). The hairy vetch + barley (60 : 40) treatment showed 63% more soil microorganisms than control. Radical scavenging activity estimation revealed that the total polyphenol content was highest (1,740 ㎎/㎏), and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 92.6% in the barley treatment. The 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) activation was highest in the control (51.1%), and the root yield was the highest in the barley treatment (310 ㎏/10 a).Conclusions:The root yield, total polyphenol content, and antioxidant activity of Cynanchum wilfordii (Maxim.) Hemsley increased in presence of the green manure crop barley.

9년생 ‘신고’배(Pyrus pyrifolia Nakai)나무 과원에서 화학비료 대체 기술을 확립하고자 녹 비작물의 예초시기와 예초높이에 따른 무기성분의 환원량을 2009년에 비교하였다. 호밀과 헤어리베치를 2008년 가을에 혼파하여 예초시기를 기준으로 총 4가지 처리를 포함하였다. 4월 18일에 1차 예초와 5월 28일에 2차 예초 한 것을 4월 18일+5월 28일 예초 처리구로 설 정하였고, 4월 28일+6월 8일 예초, 5월 8일+6월 18일 예초, 4월 18일+5월 18일+6월 18일 예 초처리를 시험에 이용하였다. 예초높이 처리는 3±1 cm, 7±1 cm, 13±1 cm 높이에서 4월 18 일과 6월 8일에 각각 예초한 것을 포함하였다. 예초 시기별 처리에서, 5월 8일+6월 18일 예 초처리가 녹비작물과 자연초종의 건물중 생산량이 ha당 14,480 kg으로 가장 높았고, 이에 따라 토양에 환원되는 전질소와 칼륨도 ha당 각각 292 kg과 396 kg으로 가장 높았다. 예초 높이 처리에서는 7±1 cm 처리에서 녹비작물과 자연초종의 건물중 생산량이 ha당 11,970 kg 으로 가장 낮아서 전질소와 인산, 그리고 칼륨이 ha당 각각 265 kg, 111 kg, 333 kg으로 가 장 낮았다. 녹비작물 처리는 예초방법에 상관없이 토양의 유기물 함량을 다소 증가시켰던 것으로 관찰되었다.

Background: Some plants have harmful effects on fungi and bacteria as well as other plants. Incorporating such plant into soil as green manure is effective in reducing population densities of soil pathogens. Methods and Results: Twenty-three species of green manure crops were cultivated after the harvest of 6-year-old ginseng and then incorporated into the soil at the flowering stage. The following year, the root rot ratio of 2-year-old ginseng and soil chemical properties were investigated. In the absence of green manure addition, the NO3 content, electric conductivity (EC), and K content decreased by 95%, 79% and 65%, respectively. In the presence of green manure addition, P2O5 and NO3 contents reduced by 41% and 25%, respectively. The “survived root ratio” of 2-year-old ginseng significantly increased by 56.2%, 47.5%, and 47.3%, in the Sorghum sudanense, Ricinus communis and Helianthus tuberosus treatment, respectively. In addition, there was a significant increase in the “survived root ratio” in the Secale cereale, Chrysanthemum morifolium, Atractylodes macrocephala, and Smallanthus sonchifolius treatments. The “survived root ratio” of ginseng showed a significant positive correlation with the soil pH and a negative correlation with the NO3 contents, and EC. Conclusions: Cultivation of plant form the Chrysanthemum family as green manure, using mainly the rhizomes was effective for the control of root rot disease of ginseng.

Background : The authors of this study analyzed the effect of the green manures cultivated in the soil of the preparation field in order to select the green manures suitable for ginseng cultivation and the effect of the green manures on the variation of ginseng growth development, the content of crude saponin and yield ability with the aim to use the results as the primary data for improving the quality of ginseng. Methods and Results: To analyze the effect of the application of green manures on the growth of ginseng, the authors cultivated 4 varieties of green manures and installed blue polyethylene sheet and one-layered black polyethylene net+three-layered blue polyethylene net(OBPNTB) and sun-shading material on the managed soil by re-applying green manures and then cultivated ginseng. The results suggested that the growth of ginseng was excellent in the barley+hairy, barely and rye cultivation plot in the plot treated with OBPNTB rather than the cultivation plot teated with Blue polyethylene sheet(BPS) and two-layered blue & two-layered black polyethylene net(TBTBPN), and the growth of leaf length and stalk diameter was excellent in barely + hairy vetch material. For the underground root weight, the growth was the most excellent(30.19g) in the barley+ hairy vetch cultivation plot treated with OBPNTB. For the underground red discoloration and root rot, the incidence rate was the lowest in the plot treated with BPS in which the water leak in a levee was small than in the test plot treated with sun-shading net. For the ginsenoside content in the cultivation plot treated with TBTBPN, the ginsenoid content was the highest in the rey cultivation plot, and it was the highest in the cultivation plot treated with OBPNTB. Conclusion : The results of study suggested rye among green manures is the most suitable for the growth of ginseng; underground growth was the most prominent in the plot treated with sun-shading net material (black 1 + blue 3) and the underground ginsenoside content was the most prominent in the rye cultivation plot and the plot treated with the sun-shading sheet.

Background : Root diseases caused by Cylindrocarpon destructans and Fusarium solani decrease the yield and quality of ginseng. Cylindrocarpon root rot is a major disease caused by replanting failure in ginseng garden. Methods and Results : Solarization was done in the infested soil of the greenhouse for summer season (from July 24 to Autumn 31, 2014) after putting green manure (Sudan grass) and calcium cyanamide (CC) into the soil. Mycelium and conidia of C. destructans died at 4 0℃ after 15 hours, and 45℃ after 5 h, but it did not die at 35℃ after 15 h. Those of C. destructans died after keeping it for 2 hours daily at 40℃ for 9 days, and 45℃ for 8 days, but did not die at 38℃ for 9 days. Maximum soil temperature was 55.4℃ in 5 cm depth, 48.7℃ in 10 cm, 44.7℃ in 15 cm, 42.5℃ in 20 cm, and 31.9℃ in 30 cm by putting green manure into the soil and solarization. Reduction of sudan grass increased electrical conductivity (EC), organic matter, P2O5, K, and Mg, while decreased pH, NO3-N, and Na. Addition of calcium cyanamide and urea gave a negative effect on the growth of ginseng because EC and NO3-N were increased excessively than the optimal range. Solarization using green manure mixed with CC was the most effective in decreasing soil-borne pathogens of 2-year-old ginseng. But the root disease that occurred between single treatment of sudan grass and the treatment mixed with calcium cyanamide showed not a significant different. Addition of calcium cyanamide showed the decrease of root weight because leaves were dead early by a excessive increase of EC and NO3-N. Conclusion: Soil disinfection using green manure and solarization in greenhouse was effective in inhibiting root rot, however, it did not completely kill the soil-borne pathogens.

Background: Root diseases caused by Cylindrocarpon destructans and Fusarium solani decrease the yield and quality of ginseng. Cylindrocarpon root rot is a major disease caused by replant failure in ginseng fields. Methods and Results: Solarization of infested greenhouse soil was carried out during the summer season after applying green manure (Sudan grass) and Calcium Cyanamide (CC) on the soil. Mycelium and conidia of C. destructans died at 40℃ after 15 h, but they did not die at 35℃ after 15 h. They also died after keeping the soil at 40℃ for 2 h daily for 9 days, and at 45℃ for 8 days, but they did not die at 38℃ for 9 days. Maximum soil temperature was 55.4℃ at 5 ㎝ depth, 48.7℃ at 10 ㎝, 44.7℃ at 15 ㎝, 42.5℃ at 20 ㎝, and 31.9℃ at 30 ㎝ by incorporating green manure into the soil and using solarization. Solarization using green manure mixed with CC was the most effective in decreasing soil-borne pathogens of 2-year-old ginseng. However, the addition of CC decreased the root weight due to the increase in EC and NO3-N. Conclusions: Soil disinfection using green manure and solarization in a greenhouse environment was effective in inhibiting root rot, however, it did not completely kill the soil-borne pathogens.

This research carried out to figure out the effect of the green manure crop cultivated at a preparation field and the shading net on the growth, development, and quality of ginseng. Followings are results obtained from the research. Leaf width of ginseng under the shading net of a two-layered blue and two-layered black polythylene net (TBTBPN) was good at rye and hairy vetch cultured group. Leaf length of ginseng under the shading net of a threelayered blue and one-layered black polyethylene net (TBOBPN) was good at barley and hairy vetch cultured group. Meanwhile, leaf width was good at hairy vetch cultured group. Leaf length of ginseng under a blue polyethylene sheet (BPS) was good at a barley and barley + hairy vetch cultured group, but stem length was shorter compare to other shading net cultivations. Root weight of ginseng was good under the shading net of a two-layered blue and two-layered black polyethylene net (TBTBPN) at a rye and hairy vetch cultured group, and was good under the shading net of a three-layered blue and onelayered black polyethylene net (TBOBPN) at a barley + hairy vetch cultured group, but there was no significant difference under blackout screen according to manure crop varieties. Ratio of rusty root was 10.2% at the barley cultured group under the shading net of a two-layered blue and two-layered black polyethylene net (TBTBPN), and was 23.1% at hairy vetch cultured group under shading net of a three-layered blue and one-layered black polyethylene net (TBOBPN). Ratio of rusty root was the lowest at a rye cultured group regardless the shading nets. Content of the ginsenoside was the highest at the rye cultured group under the shading net of two-layered blue and two-layered black polyethylene net (TBTBPN), was the highest at the barley cultured group under the shading net of a three-layered blue and one-layered black polyethylene net (TBOBPN), and was the highest at the rye cultured group under the blackout screen.

This study was conducted to evaluate the effect of organic inputs on soil properties in a newly reclaimed organic soils. The soil of the experiment site was very low in soil fertility and the physico-chemical properties were poor. Several organic input treatments with different source of nutrient were placed, including compost in combination with green manures for organic agricultural practices, chemical fertilizers for conventional agricultural practices, and control without fertilizer. The experiment was conducted with continuous cropping system during 3 years. The chemical properties concentration in compost+green manure treatment was increased continually compare to control and chemical fertilizer treatment, and closed to the recommended rate of fertilizer. The organic matter value for compost+ green manure treatment was increased from 0.86~0.96% to 2.00~2.29% by continuous nutrient supply of compost and green manure. However, further investigation on increasing of organic matter value for 3 years is necessary to monitor carefully during the long-term because it will help to clarify the all mechanisms of organic matter on organic input application way. The available phosphate value for compost+ green manure treatment was generally increased from 21.9~27.1 mg/kg to 182.0~ 394.1 mg/kg. In case of exchange cation, the concentration for compost+ green manure treatment was increased during 2 years within the range to the recommended rate of fertilizer, however, it is expected to cause a rather over supply for 3 years.

In sustainable viticulture, green manure represents a safe and non-polluting way to bring large quantities of organic matter into the soil. The cultivation of green manure crops plays an important role in soil quality and sustainability of agricultural system. This study was conducted to evaluate the effects of green manure fertilization on yield and quality of the Campbell Early grape variety in the organic vineyard. Hairy vetch was the more productive green manure crop, yielding higher dry materials. Average yields of grape were significantly greater in hairy vetch + rye (13.02 ton ha-1) than nature weed (11.65 ton ha-1), respectively. The concentrations of total as well as individual anthocyanins were consistently higher with hairy vetch treatment compared with rye and nature weed, thus making the green manure cultivation is an environmentally friendly cultivation to increase the yield and anthocyanin contents in organic grape.

생강연작재배지 토양에 녹비작물의 재배가 토양 양분의 변 화와 뿌리썩음병원균 경감효과를 알기위해 헤어리베치, 크림 손클로버, 오차드글라스를 80일간 재배 후, 토양에 환원하여 토 양분석 및 real-time PCR 분석한 결과는 다음과 같다. 녹비작 물의 생육은 크림손클로버가 가장 좋았으며, 질수흡수량은 크 림손클로버가 가장 높게 나타났으며, 토양의 인산함량은 오차 드글라스가 가장 낮게 나타났다. T-N 함량은 헤어리벳치 > 크 림손클로버 > 오차드글라스 순으로 나타났다. 또한 토양 내 뿌 리썩음 병원균 밀도분석을 위해 Pythium zingiberum 균 특이 5.8S rDNA를 이용하여 real-time PCR 분석한 결과 헤어리베 치, 오차드그라스 및 크림손클로버 처리의 Ct값은 대조구보다 낮게 나타났다. 이상의 결과를 요약해볼 때 생강연작재배지에 서 녹비처리 후 토양은 대조구에 비해 EC가 감소되고, 몇 몇 무 기성분은 증가하였으며, 뿌리썩음 병원균 밀도는 감소하였다.

본 연구는 시설하우스 내 토마토 연작장해 경감을 위해 단기 녹비작물 재배가 토마토의 생육특성 및 수량에 미치는 영향을 알아보기 위해 토마토 시설재배지에 헤어리베치와 호밀을 단파 및 혼파로 구분하여 파종하였으며, 생육된 녹비작물의 양분공 급량과 녹비작물의 토양환원 후 토양의 이화학적 특성 그리고 녹비작물 환원 후 후작물인 토마토의 생육특성 및 토마토 시들 음 병원균의 밀도를 조사한 결과, 녹비작물의 질소(N), 인(P2O5), 칼륨(K2O), 칼슘(CaO) 및 마그네슘(MgO) 공급량은 헤어리베 치의 경우 각각 26.2, 5.8, 10.2, 6.6, 및 1.5 ㎏/10a였으며, 호밀 은 각각 9.1, 4.2, 11.8, 3.8, 및 3.1 ㎏/10a였고, 혼파의 경우는 단파와 유사한 경향이었다. 토마토 시설재배지에서 녹비작물 의 토양환원 전･후 토양의 pH는 대조구와 별다른 차이 없이 6.37-6.52 범위였으며, EC는 헤어리베치(2.64 dS/m) 및 호밀 (2.62 dS/m) 처리가 대조구(헤어리베치-2.80 dS/m, 호밀 -2.91 dS/m)에 비해 낮았다. 토양 중 유기물, T-N 및 avail. P2O5 함량은 녹비작물 처리가 대조구에 비해 증가하는 경향이 었다. 녹비작물 토양환원에 따른 후작물인 토마토 생육은 녹비 작물 처리는 대조구에 비해 우수한 생육을 보였으나, 관행처리 와는 비슷한 생육을 보였다. 또한 녹비처리에서 토마토 시들음 병원균의 밀도가 현저하게 감소하였다. 이상의 결과를 종합할 때, 토마토 시설재배지에서 녹비작물의 시용은 비료로서 충분 한 가치가 있으며, 녹비작물 토양 환원 후 토양화학성을 개선함 으로써 시설하우스 토마토 연작재배지의 연작장해를 경감할 수 있을 것으로 사료된다.

Stable production of green manure crop is one of the critical factor for successful implementation of environmental-friendly agriculture in South Korea. Most green manure crops including hairy vetch cultivated in South Korea must be passed cold winter season. This study was conducted to establishment of cultivation zones on green manure crops under paddy field in South Korea using on minimum temperature and drainage class. In order to build the spatial distribution map, hairy vetch, triticale, barley, rye, and clover as green manure crop were selected and mean of daily minimum temperature and drainage class in paddy field were considered as limiting factor for stable growth of green manure crop. And cultivation zone on green manure crops was divided into two categories; ‘Availability area’ and ‘Safety area’. Low limit temperature on green manure crops in this study was the following. Temperature on ‘Availability area’ was –10℃, –8℃, –6℃, and –20℃ for hairy vetch (including triticale), barley, clover, and rye respectively. And temperature on ‘Safety area’ was lower –2℃ compared to that of ‘Availability area’ exception rye which was the same degree. Rye was expected to safely cultivated the almost whole paddy field (99.71%) of South Korea. ‘Safety area’ among the whole paddy field for hairy vetch, barley, and clover were 86.58, 53.06, and 15.76% respectively. The spatial distribution map of green manure crops reestablished in this study could be used the policy and agricultural management for environmental-friendly agriculture using green manure crops in South Korea.

녹비작물로서 녹비보리와 헤어리베치를 환원하고, 토양개량제로서 charcoal을 시용하여 생강연작 재배지에서 생강을 재배한 결과, 토양 pH가 시험 전 6.9~8.1에서 수확 후 6.8~7.6로 감소하였으며, 전기전도도(electrical conductivity, EC)는 한 시험 전 0.45~1.25dS m-1에서 수확 후 0.30~0.61dS m-1로 감소하였다. 토양유기물(soil organic matter, SOM) 함량과 토양 중 총질소(total-nitrogen, T-N) 함량은 증가하였다. 유효인산 함량과 치환성 K, Ca, Mg 함량은 유의성 있는 차이가 나타나지 않았다. 그러나, 토양 pH를 중성화시키고, EC 경감, SOM과 T-N 함량의 증가는 토양 화학성 변화에 있어 긍정적으로 평가할 수 있다. 생강양분의 함량적 차이를 정상근과 이병근의 함량 차이, 토양의 점토함량 별 양분 함량 차이로 조사한 결과, 유의성이 있는 결과를 얻었으며, 처리구별로 차이가 있는 것으로 나타났다. 특히, 망간(manganese, Mn)의 경우, 이병근의 지하부에서 3~4배 정도 높은 것으로 나타났는데, 망간(Mn)은 식물 뿌리에 대한 독성 작용으로 뿌리를 갈변시키고 균열을 일으키는 것으로 보고된바, 뿌리썩음병의 발생으로 Mn이 작용하여 이병근의 Mn 함량이 높은 것으로 보인다. 따라서, 뿌리썩음병과 Mn의 상관관계를 밝히는 추가 연구가 필요할 것으로 판단된다. 또한, 생강 수확 후 생강 생육 상태와 생산량을 조사한 결과, 녹비보리, 헤어리베치, 녹비보리+charcoal, 헤어리베치+charcoal을 처리한 시험구(Plot 2, Plot 3, Plot 4, Plot 5)에서 생강의 생육상태가 양호하고 생산량이 높아 병발생률이 상대적으로 낮게 나타났다. 특히, Plot 4(헤어리베치+charcoal)-LCC에서는 100%의 생산량을 보였다. 녹비작물과 charcoal의 처리가 생강 생육과 생산에 있어 긍정적인 영향을 미친 것으로 판단된다. 그리고, 상대적으로 점토함량이 낮은 처리구가 점토함량이 높은 처리구보다 생육 상태와 생산량에서 우세하였는데, 모래와 점토 함량 차이에 따른 배수와 토양의 수분보유력이 생강생육에 큰 영향을 준 것으로 판단된다.

‘부유’ 단감과수원에 적합한 녹비작물을 선발하기 위하여 헤어리베치, 레드클로버, 호밀, 헤어리베치+호밀을 파종하여 토양화학성과 물리성 그리고 과실수량과 품질을 조사하였다. 천근성인 레드클로버는 생육이 저조하여 피복도가 약 66%로 처리구에서 가장 낮았고 호밀 또는 헤어리베치+호밀 혼파처리구의 토양환원량이 가장 높았다. 식물체에서 환원되는 질소, 인산, 칼리 함량이 헤어리베치+호밀처리구 혼파처리구에서 가장 높아 토양의 유기물과 pH, 인산, 칼리, 칼슘과 전기전도도가 증가하였다. 토양의 용적밀도와 고상이 호밀 또는 헤어리베치+호밀처리구에서 감소되어 기상의 증가로 인한 공극률이 증가되었다. 헤어리베치+호밀처리에서 과실의 착과수와 수량이 가장 높았으며, 과실의 당도와 착색이 증가되는 효과가 인정되어 녹비작물로서 단감과원에 이용될 수 있을 것으로 판단된다.

This study investigated ginseng growth and ginsenoside contents after control a reserved ginseng cultivation land using various green manure crops for stable ginseng cultivation. Followings are results obtained from this research. After cultivate the green manure crops, microbial flora in soil was diversified, organic matter and total-N content increased, but salt content decreased. The highest output obtained from the wheat cultivated area among various green manure corps. Growth of shoot and root of two years old ginseng increased significantly at the green manure crop cultivated area. Specially, the wheat cultivated area was the most effective in growth. Also, the rate of the leaf discoloration at the aerial part and the rusty root at the root was the least at the wheat cultivation area. Meanwhile, the ginsenoside content was the most at the wheat cultivation area. Thus, the reserved ginseng cultivation land could be managed by cultivating wheat for effective ginseng growth.