오이, 토마토 고추 작물의 고온기 플러그 육묘시 묘의 도장 방지와 과번무 억제를 위한 UV-B와 생장 억제제의 처리가 플러그묘 식물체의 생리적 특성 변화와 항산화 효소의 활성에 미치는 영향을 조사하였다. 광합성능력은 UV-B 처리묘의 경우 무처리에 비해 처리 직후에는 크게 떨어졌으나 10일 후에는 비스하게 회복되었고 생장억제제 처리묘는 무처리에 비해 토마토가 다소 떨어졌어나 오이와 고추는 비슷하였다. 엽록소함량은 3작물 모두 처리간에 유의한 차이가 없었다. 안토시아닌 함량은 무처리에 비해 UV-B 처리한 것이 다소 높았던 반면 생장억제제 처리한 것은 무처리와 비슷하였다. SOD, CAT, POD 등의 항산화효소의 활성은 3작물 모두 UV-B 처리한 것이 가장 높았으며, 처리 직후에는 크게 증가되었다가 10일 후에는 그 절반 이하로 감소하였다. 한편 생장억제제 처리묘는 무처리에 비해서 항산화 효소의 활성이 다소 높았으나 UV-B 처리묘에 비해서는 낮았다.
대두유를 기초로 한 튀김유에 capsicum. butter flavor, 규소수지를 각각 0.20, 0.15%(ω/ω) 및 10ppm 처리하여 가열안정성의 향상 및 육두향 약화에 효과가 있는 것으로 확인되었다. 즉, capsicum과 규소수지를 병행처리한 튀김유는 185±2℃의 온도조건 하에서 3시간 동안 연속적으로 열처리할 경우 AV 및 SP가 각각 무처리군의 0.385, 220℃에 비하여 크게 향상된 0.301, 232℃를 나타내었다. 이러한 효과는 capsicum의 항산화 효과와 함께 소포제의 일종인 규소수지의 작용에 따라 가열처리에 따른 튀김유의 표면적 팽창을 방지하여 유리지방산 생성 및 연기발생을 억제한데 따른 효과인 것으로 판단되었다. 또한, 0.15%(ω/ω)의 butter flavor처리에 따라 튀김유 및 튀김물에서 발생하는 육두향 및 기름타는 냄새를 막아주는 효과가 인정되어 새로운 형태의 튀김유 제품 생산이 가능할 것으로 기대되었다.
The antioxidative, radical scavenging and cyto-protective effects of Cassia torn L. seeds and it major component, nor-rubrofusarin-6-β-D-glucoside (nor-rubrofusarin), were studied to evaluate their inhibitory activity against hydrogen peroxide-induced oxidative stress. 70% ethanol extract of Cassia tora L. seeds and nor-rubrofusarin also were tested for the evaluation of anticlastogenicity against mitomycin C-induced micronucleated reticulocytes in mouse peripheral blood. The extract of Cassia tora seeds and nor-rubrofusarin showed an antioxidative effect on the lipid peroxidation of ethyl linoleate with Fenton's reagent and free radical scavenging effect to DPPH radical generation, respectively. They showed a protective effect against H₂O₂-induced cytotoxicity in CHL cells. The extract of Cassia tora L. seeds and nor-rubrofusarin showed a strong anticlastogenicity against mitomycin C-induced micronuclei formation. Results from our study indicate that the extract of Cassia tora L. seeds and nor-rubrofusarin are capable of protecting the lipid peroxidation, free radical generation and cytotoxicity induced by reactive oxygen species. They also have an anticlastogenicity toward DNA crosslinking agent like mitomycin C.
Panax ginseng C.A. Meyer has been extensively used in the traditional oriental medicine as a restorative, tonic and prophylatac agent. This study was devised to develop a chemopreventive agent from panax ginseng to be able to suppress the genotoxicity and oxidative damage by reactive oxygen species, which are involved with cancer or aging. Ginseng petroleum ether extract (GPE) and one of its fraction, P2, showed an antioxidative effect on the lipid peroxidation of ethyl linoleate with Fenton's reagents and free radical scavenging effect to 1,1-diphenyl-2-picryl hydrazil (DPPH) radical generation. They also showed the suppressive effect of H₂O₂ or KO₂ induced DNA damage by single cell gel electrophoresis (SCGE). Results from our study indicate that GPE and P2 are capable of protecting lipid peroxidation, and oxidative DNA damage. Therefore, GPE and P2 may be useful chemopreventive agents which are involved with cancer and aging.
The antilipidperoxidative and hepatopreventive effects of Aloe water extract (30 mg, 50 mg, 100 mg) were investigated at the levels of liver-total homogenates and the sera of SDrats intoxicated with CCl₄, (0.5 cc/100 g) and 50% ethanol. We measured MDA (Malondialdehyde) in the liver homogenate, AST (L-Aspartate-2-oxoglutarate aminotransferase) and ALT(L-Alanine-2-oxo-glutarate aminotransferase) in the serum. The analysis of the measurement indicated that Aloe water extract reduced MDA, ALT and AST significantly and their reduction was in relation to dose dependence. In rat liver homogenate intoxicated with ethanol and CCl₄, Aloe treatment group markedly inhibited lipidperoxidation by 30%-70%. In rat serum intoxicated with ethanol and CCl₄, Aloe treatment group inhibited AST, ALT by 40%-90%. In these data Aloe may be used to inhibit or prevent the hepatic toxicity which results from the environmental and alcoholic factors through the further study of its exact antihepatotoxic mechanism.
탄소/탄소 복합재의 산화 저항성을 개선시키기 위하여 aluminum iso propoxide및 aluminum tri sec butoxide졸을 2D-탄소/탄소 복합재에 도포하여 산호 억제층으로서의 효과를 관찰하였다. 촉매/알콕사이드의 몰비가 0.07, 물/알콕사이드의 몰비가 100일때의 산화 억제효과가 양호했으며, 승온속도를 20˚C/min로 하여 승온분석시험한 결과는 도포시편이 80˚C 정도의 산화 개시온도가 20%감소되는 시간을 측정한 TGA분석에서는 도포시편이 20% 정도의 산화 저항성 개선효과를 나타냈다. 도포막의 두께는 1회 도포막이 3μm, 2회 및 3회 도포막이 4-5μm 정도였고, 열충격 시험은 횟수에 따라 산화량이 증자하였다. 5% 전환률에서의 도포하지 않은 시편의 활성화 에너지는 33.2Kcal/mole이었으며 도포시편의 활성화 에너지는 37.1Kcal/mole이었다.
pMOS소자의 p+게이트 전극으로 다결정실리콘과 비정질실리콘을 사용하여 고온의 열처리 공정에 따른 붕소이온의 침투현상을 high frequency C-V plot, Constant Current Stress Test(CCST), Secondary Ion Mass Spectroscopy(SIMS) 및 Transmission Electron Microscopy(TEM)를 이용하여 비교하였다. C-V plot분석 결과 비정질실리콘 게이트가 다결정실리콘 게이트에 비해 flatband전압의 변화가 작게 나타났으며, 게이트 산화막의 절연파괴 전하밀도에서는 60~80% 정도 향상된 값을 나타내었다. 비정질실리콘 게이트는 증착시 비정질로 형성되는 구조로 인한 얇은 이온주입 깊이와 열처리 공정시 다결정실리콘에 비교하여 크게 성장하는 입자 크기 때문에 붕소이온의 침투 경로가 되는 grain boundary를 감소시켜 붕소이온 확산을 억제한 것으로 생각된다. Electron trapping rate와 flatband 전압 변화와의 관계에 대하여 고찰하였다.
Background: The plant Aster koraiensis has long been used as an ingredient in folk medicine. It has been reported that Aster koraiensis extract (AKE) prevents the progression of diabetes-induced retinopathy and nephropathy. However, although these beneficial effects of AKE on diabetes complications have been identified, the antidiabetic effects of AKE have not yet been completely investigated and quantified. In the present study, the glucose-lowering and antioxidant effects of aqueous and ethanolic AKEs were evaluated.
Methods and Results: The glucose-lowering effects of aqueous and ethanolic (30%−, 50%−, and 80%-ethanol) AKEs were investigated via α-glucosidase inhibitory assays. The mode of inhibition by AKEs on α-glucosidase was identified through kinetic analysis. The total antioxidant capacity of each of the 4 AKEs was evaluated by assessing their conversion rate of Cu2+ to Cu+. The content of chlorogenic acid and 3,5-di-O-caffeoylquinic acid, the bioactive compounds in AKE, in each extract were analyzed by high performance liquid chromatography (HPLC). The AKEs showed potent α-glucosidase inhibitory activity with mixed inhibition mode, and significant antioxidant capacity.
Conclusions: These results of this study suggested that the AKEs tested had α-glucosidase inhibitory and antioxidant effects. Among the extracts, the 80% ethanol extract showed the most significant α-glucosidase inhibitory activity, with a half maximal inhibitory concentration (IC50 value) of 1.65 ± 0.36㎎/㎖ and a half maximal effective concentration (EC50 value) for its antioxidant activity of 0.42 ± 0.10㎎/㎖. It can therefore be used as a source of therapeutic agents to treat diabetes patients.
Background : Stevia rebaudiana (Asteraceae), a perennial plant, has been used as a low caloric sweetener and therapeutic agent for diabetes, hypertension, myocardial, and antimicrobial infections. It has been commonly used leaves and stems because of their high anti-oxidative potential. The present research was carried out to explore anti-oxidative and anti-melanogenic effects of aqueous extract of Stevia rebaudiana Bertoni flos in B16F10 cells.
Methods and Results : Anti-oxidant activity of Stevia flos extract (SFE) was determined by using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging assay, nitric oxide (NO) radical scavenging method, and reducing power method. The results showed that the total phenolic content of SFE was 63.154 ± 0.0002 ㎍·QE/100㎎ and the total flavonoid was 8.64 1 ± 0.002 ㎍·GAE/100㎎. SFE exhibited a big significant effect on NO radical scavenging activity (IC50: 179.6 ㎍/㎖) comparing with standard ascorbic acid (IC50: 368.6 ㎍/㎖), and showed concentration dependent DPPH radical scavenging activity (IC50: 131.8 ㎍/㎖). Anti-melanogenic effect of SFE was also examined with B16F10 melanocytes. The amount of melanin synthesis followed by α-melanocyte stimulating hormone on B16F10 cells were significantly reduced in the presence of SFE treatment (p < 0.05). SFE also suppressed the tyrosinase activity (p < 0.05) and α-glucosidase activity (p < 0.05).
Conclusion : These results provide evidence Stevia rebaudiana flos has an antioxidant potency and can be used as an anti-melanogenic agent.
Background : Obesity is a type of metabolic diseases caused by unbalanced in take and consumption of calories. 3T3-L1 is differentiated into adipocytes by various hormones and transcription factors and accumulates intracellular lipid. Therefore, it is important to inhibit the adipocyte differentiation precess for effective obesity inhibition. Aster yomena and Aster glehui are medicinal plant of Compositea family that grows widely in Korea. Aster genus plants have been used to treat snakebite wound or bruises in oriental. The aim of this study was comparison of inhibitory effect oxidation and adipocyte differentiation with Arial parts of A. yomena (AY) and A. glehni (AG).
Methods and Results : AY and AG were cultivated from Pyeongchang in Korea, 2018. AY and AG were extracted by 70% ethanol (-E) and water (-W) at room temperature. AG-W has higher phenolic content (6.92 ± 0.23) and AG-E has higher flavonoid content (8.22 ± 0.19) than other extracts. AG-E has higher radical scavenging activity on DPPH and ABTS assay (IC50 value; 104.88 ± 10.50 and 30.06 ± 0.27). In cytotoxity assay, all extracts concentrations of lower 100 ㎍/㎖ were nontoxic to the cells and can be applied for the next assay. The anti-adipogenic effect of extracts were determined in 3T3-L1 cell by Oli Red-O (ORO) staining. The lipid diplot stained with Oil red O was dissolved to determine by microplate-reader. AG-W significantly reduced the adipocyte differentiation of 3T3-L1 cells (70.49%) compared with other extracts (AG-E, AY-W and AY-E).
Conclusion : Theses results reveal that the water extract of AG has utility as a functional food material for preventing obesity.
Background : Exposure to Ultraviolet B (UVB) causes oxidative stress, inflammation, pigmentation and severe skin damage. Astragalus membranaceus (AG) has been used as a traditional medicine and have been studied various physiological activities. During the roasting process, bioactive substances is change including antioxidant substances. The aim is study the antioxidant effects and reactive oxygen species (ROS) inhibitory effect of the roasted A. membranaceus (R-AG). on Human dermal fibroblast (HDF) cells.
Methods and Results : To prepare of R-AG samples, roasting machine was used. AG and R-AG were extracted to water and 70% ethanol. AG samples were evaluated the antioxidant potential by measuring the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2`-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) scavenging activities. Additionally, total phenolic contents and total flavonoid contents was compared with antioxidant ingredients. AG and R-AGs were analyzed with HPLC determine the major compounds such as calycosin, mononetin and glycosides. The antioxidant activities of R-AG increased and changed in major compounds. In UVB exposed HDF cells, AGs did not affect cell viability and R-AG inhibited ROS more effectively than AG.
Conclusion : From these results, R-AG can inhibit oxidative stress induced UVB in HDF cells.
Background : Scopolamine induces cholinergic dysfunction and oxidative stress, and the impairment of memory function. Therefore, oxidative stress and cholinergic dysfunction are important role of the brain pathology of amnesia. In this study, we investigated the impact of Safflower seed against oxidative stress and cholinergic dysfunction on scopolamine-induced amnesic mice.
Methods and Results : Mice were orally pretreated with safflower seed (100 ㎎/㎏ body weight) or vehicle for 7 days, and scopolamine (1 ㎎/㎏ body weight) was injected intraperitoneally, 30 min before the behavior tests such as T-maze and novel objective recognition test on first day. To evaluate learning and memory function, the Morris water maze task was performed for 5 days, consecutively. The results showed that spatial perceptive ability and novel object recognition was significantly increased by preadministration of safflower seed compared with scopolamin-induced control mice in the behavior tests. Consistently, immuno blot revealed the elevated expression of superoxide dismutase 1 in the safflower seed pretreated mice, compared to the control mice. Moreover, protein expression of acetylcholinesterase was decreased in safflower seed pre-treated group.
Conclusion : Subsequently, our results suggests that the Safflower seed extract improved memory impairment through inhibition of cholinergic dysfunction and oxidative stress.
Background : Oxidative stress and inflammatory response are important features of the brain pathology of Alzheimer's disease. Therefore, the purpose of this study was to the antioxidant activity and biochemical characterization of safflower seed. Moreover, we investigated the impact of Safflower seed on scopolamine-induced memory impairment in mice.
Methods and Results : First, in order to determine active ingredient contents of safflower seed extract, we were carried out total phenol content and total flavonoid content analyses. As a result, dried safflower seed were found to contain 35.4 ± 0.4 ㎎·GAE/g dry weight and 45.3 ± 7.5 ㎎·NE/g dry weight in boiling water extraction. Also, the major compounds of safflower seed from HPLC analysis were identified as serotonin and serotonin derivatives [N- (p-coumaroyl)serotonin and N-feruloylserotonin]. In addition, the antioxidant activity of safflower seed showed IC50 values of 331.4 and 168.2, respectively, against DPPH and ABTS in vitro. Finally, with regard to the memory improvement activity, the administration of Safflower seed extract significantly restored memory impairments induced by scopolamine in the behavior tests such as novel object recognition and Morris water maze test.
Conclusion : The results of our study suggest that the safflower seed extract possess potent memory improvement activity and are also a good source of natural antioxidants. Further study is needed to identify the mechanism responsible for their memory improvement activity.
Background: Astilbe chinensis (Maxim.) Franch. Et Savat. is a plant belonging to Saxifragaceae family and contains various active ingredients including astilbin and bergenin. It has been used as a traditional Korean medicine to improve fever, pain, and cough. Recently, a number of Korean medical resources have been studied for cancer and inflammation treatment, but A. chinensis (Maxim.) Franch. Et Savat. has not yet been investigated. Consequently, this study investigated the inhibitory effect of ethanol extracts from A. chinensis (Maxim.) Franch. Et Savat. (ARE) on oxidative stress and colorectal cancer using RAW264.7 and the human colorectal cancer cell line HCT-116.
Methods and Results: In total, 500 ㎍/㎖ ARE reduced cell viability by 38.96 ± 1.32%, and increased caspase-3 activity by 133.08 ± 3.41% in HCT-116 cells. Moreover, TUNEL signaling and the early apoptosis ratio (34.56 ± 1.67%) increased by 500 ㎍/㎖ ARE treatment. H2O2-induced oxidative stress and cell death were diminished by 500 ㎍/㎖ ARE treatment through decreasing ROS (reactive oxygen species).
Conclusions: The inhibitory effects of ARE against human colorectal cancer cells is mediated by apoptosis and caspase-3 activation, and H2O2-induced ROS generation and cell death are decreased by ARE treatment in RAW264.7 cells. However, further study is required to explore how ARE treatment is involved in the signaling pathway to decrease ROS.
Rubiae radix is root of Runia akane Nakai, it has been used to hemostasis and blood stasis in Korean and China. This study investigated that anti-oxidant and anti-colorectal cancer effect of ERA (ethanol extract of Rubiae radix) and WRA (water extract of Rubiae radix) using RAW 264.7 (murine macrophage from blood) and HCT-116 cells (human colorectal cancer cell line). ERA contained polyphenol (45.77 ± 2.03 ㎎/g) and flavonoid (22.82 ± 1.33 ㎎/g). 500 μM H2O2-induced ROS generation was diminished by 500 ㎍/㎖ ERA treatment in RAW 264.7 cells, but not WRA (125, 250, and 500 ㎍/㎖). Moreover, caspase-3 activity and DNA fragmentation increased by 500 ㎍/㎖ ERA treatment during apoptotic cell death in HCT-116. Results demonstrated that anti-cancer effect of ERA against human colorectal cancer cells is mediated apoptotic cell death and DNA fragmentation through caspase-3 activation. However, further study is required to what active ingredient of ERA are important for anti-oxidant and anti-colorectal cancer effect in vivo.
Background: Inula japonica Thunb. is a plant belonging to the family compositae. Inulae flos (flower of I. britannica var. chinensis Regal.) is the dried flower of I. japonica Thunb. and contains various flavonoids (patulitrin, nepitrin and kaempferol), which have been utilized in traditional oriental medicine to treat nausea, phlegm, and coughs. However, ethanol extract of I. britannica (IJE) has not been previously studied for its use in cancer treatment, and its effects on oxidative stress, or inflammation. Thus, the present study investigated the anti-oxidant, anti-inflammatory, and anti-colorectal cancer effects of IJE using RAW264.7 and HCT- 116 cells, which are human colorectal cancer cell line. Methods and Results: IJE contained flavonoids (80.95 ± 5.3 ㎎/g) and polyphenols (310.53 ± 10.6 ㎎/g). Moreover, it reduced lipopolysaccharide (LPS)-induced nitric oxide (NO) production and H2O2-induced oxidative stress by decreasing reactive oxygen species (ROS) levels. Additionally, the 500 ㎍/㎖ IJE treatment increased caspase-3 activity and apoptotic cell death in HCT-116 cells. Conclusions: These results demonstrate that the anti-cancer effect of IJE against human colorectal cancer cells involves caspase-3 activation and apoptotic cell death. IJE also inhibited LPS-induced NO production, and H2O2-induced oxidative stress in RAW264.7 cells. However, further studies are required to explore how IJE treatment regulates signal transduction in NO and ROS production.
Paeonia japonica is a perennial flowering plant used in traditional medicine therapy. The purpose of this study was to investigate the effect of water extract and solvent fractions obtained from P. japonica on anti-oxidative, anti-thrombin, anti-invasive and pro-apoptotic activities in YD-10B cells, human oral squamous carcinoma cell line. Water fraction revealed the highest extraction yield at 11.44% (w/w). Anti-oxidative activity was the highest in ethyl acetate fraction (85.13%). In the thrombin inhibitory activity test, ethyl fraction was the highest, with a value of 87.54%. Release and activation of MMP-2/pro-MMP-2 ratio in thrombin-treated YD-10B cells were significantly inhibited in the ethyl acetate fraction. At a concentration of 120 ㎍/㎖, water extract and solvent fractions of P. japonica inhibited cell proliferation in YD-10B cells except water fraction. Pro-apoptotic effect on human oral squamous carcinoma cell using the Bax/Bcl-2 ratio analysis was higher in water extract than other fractions. These findings suggest that the ethyl acetate fraction of P. japonica potentiates a promising antioxidant, anti-thrombin and anti-invasive agents.