본 연구에서는 쓴메밀 74개 유전자원의 종자 표현형 및 화학 형과 관련된 8개 주요형질을 평가하였으며, 그 결과를 바탕으로 주성분분석 및 군집분석을 수행하였다. 또한 rutin 고함유 자원 등 기능성 쓴메밀 육성재료로 활용가능한 유망 유전자원을 선발하였다. 쓴메밀 유전자원의 종자크기는 일반메밀보다 작은 평균 5.2 × 3.4 ㎜였으며, 종피색은 흑갈색이 45.9%로 가장 많았다. 종자모양은 달갈형과 타원형이 주를 이루었다. 쓴메밀 종자의 유용성분 평균 함량은 rutin이 1,393 ㎎/100 g DW였다. Flavonoid 함량 범위는 253-2,669 ㎎/100 g이었으며, polyphenol 함량 범위는 209-1,823 ㎎/100 g 으로 나타냈다. 쓴메밀 유전자원의 주성분 분석 결과, 제3주성분까지 적용하였을 때 전체 분산의 68.55%를 설명할 수 있었다. 제1주성분에는 rutin, flavonoid 및 polyphenol, 제2주성분에서는 종자길이, 제3주성분에서는 종자폭의 비중이 큰 것으로 나타났다. 이를 바탕으로 5개의 군집으로 구분할 수 있었으며, 유전자원을 구분하는 가장 중요한 형질로는 rutin, flavonoid 및 polyphenol이었다. 쓴메밀 유전 자원 중 5개 자원(HLB1004, HLB1005, HLB1007, HLB1009, HLB1013)이 높은 rutin 함량을 보였으며, 이러한 유용자원들은 향후 기능성 육종소재 개발에 효과적으로 활용가능할 것으로 기대된다.

본 연구는 충청북도농업기술원 대추연구소에서 보유 중인 대추 유전자원 중 형태적 다형성을 나타내는 ‘일본’과 주요 대추 유전자원의 형태형질을 조사하고, 대추 육종 소재로서 ‘일본’의 가능성을 확인하기 위해 수행되었다. ‘일본’은 주요 대추 유전 자원과 비교하여 엽과 과실의 크기가 뚜렷하게 작고 당도가 낮았다. 하지만 과실에 폴리페놀 함량이 가장 높았으며, 항산화 능력 또한 가장 높았다. 또한 ‘일본’은 종자형성능이 가장 높았다. 대추 유전자원들의 형태형질을 이용하여 분석한 결과, 지리적 기원에 따라 유전자원들이 분류되었다. 특히 ‘일본’은 한국 및 중국 유전자원들과 명확히 구분되었다. 따라서 종자형성능이 우수하고 기능성 성분이 높은 ‘일본’은 추후 대추 유전연구 및 교배육종을 통한 품종 육성에 유용하게 이용될 수 있을 것으로 생각된다.

Background: Various Schisandra chinensis (SC) varieties grow in diverse regions in Korea. However, there is no valid scientific evidence of these varieties. This study aimed to select the excellent resources in terms of the growth characteristics, antioxidant activities, and analysis of the active compounds of the SC collection. Method and Results: In total, 154 resources of SC were collected from various regions of Korea. The growth characteristics were measured by the number of fruit bunches, fruit number, and weight of 100 fruits. The antioxidant activities were investigated by analyzing the total flavonoid and total polyphenol contents and the radical scavenging activity of DPPH and ABTS. Schizandrin A, schizandrin c, gomisin a, and gomisin N were analyzed by HPLC. Each resource showed different growth characteristics. Among the antioxidative effects, the highest 20 resources showed high antioxidant activities in selected 29 resources. Analysis of the SC lignan index showed that all resources contained more than 1.16% of active compounds. Conclusions: All of the selected 29 SC resources were shown to have excellent growth characteristics, antioxidant activities, and bioactive compound richness. Especially, SC-004, SC-007, and SC-154 showed the best growth characteristics, and SC-22, SC-40, and SC-45 showed the best antioxidant activities and bioactive compound richness.

In Korea, common and Tartary buckwheat are cultivated mainly in spring and fall, however the available buckwheat varieties are still very limited. In this study, we have evaluated buckwheat germplasm for agronomic traits and compared flavonoids contents in different cultivation period and collection area. In common buckwheat, the number of days from sowing to flowering was 40 and 31 days and from sowing to maturity took 90 and 69 days in spring and fall cultivation, respectively. The number of nodes and branches were higher in spring cultivation while the hundred seed weight was higher in fall cultivation. The average flavonoids contents in common buckwheat were 0.20 ㎎/g dry weight (DW) and 0.40 ㎎/g DW in spring and fall cultivation, respectively. The highest flavonoids content was detected in Jeonnam accessions with 0.29 ㎎/g DW and 0.43 ㎎/g DW during spring and fall cultivation, respectively. The flavonoids contents were varied from 1.5 to 2.5 times according to the collection area. These results suggest that the agronomic traits and flavonoids contents were vary depending on the cultivation environment and germplasm collection area. Therefore, it is necessary to select the material by considering the characteristics of the germplasm for breeding of new varieties.

Background : The purpose of this study is to select potential genetic resources from safflower germplasm collected from India based on their oil compositions and agronomic characteristics. Methods and Results : The agronomic characteristics were measured during the growing period of the safflower. Total oil contents were recovered by Soxhlet extraction and the fatty acid compositions were analyzed by using gas chromatography. The mean of plant height, leaf length and leaf width were 100.19 ㎝, 20.49 ㎝, and 7.29 ㎝, respectively. The percentage of leaf margin with serration was 95%, and 2% of the total resources didn’t have spines on the involucral bract. K185681 had no spines on the involucral bract and the plant height was the smallest. 73% of the flower of safflower was yellow. 68% of safflower germplasm changed flower color from yellow to red. Total oil contents of 267 safflower accessions showed a significant variability among the entire domain of collections and ranged from 5.81 to 38.91%. Palmitic and stearic acid were ranged from 4.98 to 6.65%, and 1.82 to 2.73%, respectively. Oleic and linoleic acid showed a wide variation which ranged from 10.53 to 22.27%, and 69.46 to 81.26%, respectively. Linolenic acid was ranged from 0.06 to 0.13%. K185639 and K185639 had the highest total oil contents and linoleic acid, respectively. Cluster analysis based on oil composition and agronomic characteristics data divided the germplasm collections into three groups. Group Ⅲ having 114 accessions contained accessions with taller plant height than the other groups. Group Ⅱ having 68 accessions, the main color of flower was white but the other groups were yellow. Oleic acid showed a strong negative correlation (r = -0.9691**) with linoleic acid. Principal component analysis (PCA) based on the oil compositions and agronomic characteristics data revealed that the first two principal components (PC1 and PC2) together explained 36.28% total variation. Conclusion : These results showed that K185681, K185639 and K185639 could be useful to develop breeding and functional food.

Background : Carotenoids are pigments that are found in plants, fruits, bacteria, and fungi. β -carotene and canthaxanthin are orange pigments among thousands of carotenoids that possess potent antioxidant activity. The objective of this study was to determine β-carotene and cantaxanthin in 55 Kimchi cabbage germplasm using liquid chromatography mass spectrometry (LC-MS) method. Method and Results : 55 Kimchi cabbage accessions were sown in plug trays in the end of August, 2016. All the tissue samples were freeze-dried for 1 week and ground into fine powder and stored at -20℃ until analysis. Crude carotenoids from freeze-dried materials (100 mg) were extracted using 1 ㎖ of 100% (v/v) hexane in 10 minutes by using sonication bath followed by centrifugation. The average β-carotene contents was 1.43 ㎎·㎏-1 and ranged from 0.76 (IT 120045) to 2.25 ㎎·㎏-1 (IT 100378). The average canthaxanthin content in the entire domain of sample was 0.59 ㎎·㎏-1 with a range between 0.36 (IT 32746) and 1.08 ㎎․㎏-1 (IT 100386). Canthaxanthin content was significantly positively correlated with β-carotene (r = 0.65**) and leaf length (r = 0.63**). However, canthaxanthin was negatively correlated with cotyledon color (r = -0.41**). Principal component analysis results of the first two components (PC1 and PC2) explained 44.53% of the point variability. Conclusion : The resources with the highest β-carotene and canthaxanthin content are IT 100378, 100386, 100391, and 110828. This study could be useful to select a potential sources of health beneficial carotenoids (β-carotene and canthaxanthin) in Kimchi cabbage germplasm in nutraceutical formulations and for further applications as a breeding material and other research activities.

Background : Angelica dahurica, Ligusticum sinense and Ledebouriella seseloides are cultivated in small areas in Korea. Domestic cultivation area is around 10 ha, and cultivated varieties have not been developed yet. Therefore, the quality of the product is not uniform, and industrialization is difficult. Lately, studies on the development of varieties have been carried out using domestic collecting germplasm. Methods and Results : Germplasm was collected in Gangwon-do, Chungcheongbuk-do, Gyeongsangbuk-do, Jeollanam-do and Jeju-do and sowed in pots in March 2017. Angelica dahurica was used the collected in Gyeongsangbuk-do, Jeollanam-do, Ligusticum sinense was used the collected in Gangwon-do and Gyeongbuk-do and Ledebouriella seseloides was used the collected Jeju-do. In May of 2017, those were planted and the stem and leaf characteristics were compared and analyzed 1-year. It was bloomed in the second years of 2018, and stem, leaf and flowering characteristics were compared and analyzed. In the first year of growth characteristics, Angelica dahurica showed lower leaf and stem growth in Jeollanam-do collected germplasm. However, there was no statistical significance. Growth characteristics of Ligusticum sinense were higher in Gangwon-do collection than in Gyeongsangbuk-do, but there was no statistical significance. Ledebouriella seseloides was no significant difference between the groups in Jeju-do. In the second years, individual selection were carried out for popullation improvement. The Angelica dahurica showed green and purple stalks and the collected germplasm of Jeollanam-do showed relatively low growth. Ligusticum sinense and Ledebouriella seseloides were no significant differences in growth and color between stem and leaf, stem and stem Conclusion : All three crops were cross pollination, and collected open pollination. Therefore, there were many segregation by germplasm and genetic variation was large. Furthermore, it is considered that statistical significance does not appear. If genetic uniformity is increased through continuous selection and population improvement, it will be possible to cultivate varieties using germplasm. Genetic variation will be a good source of genetic resources.

Background : Licorice has been a medicinal crop which was mainly used as a traditional medicine and food, depends on most imports (99%) in Korea. We have been trying to produce licorice in Korea for a long time. However when it grow in Korea the main ingredients are below the standard value and occur of physiological disorder. Therefore, we evaluated the growth characteristics and major components of genetic resources to develop licorice varieties suitable for the domestic environment. Methods and Results : We collected 19 accession of licorice from 7 regions including China, Mongolia, Uzbekistan, and Russia from 2013 to 2014. After regenerating the resources of licorice in the field we identified and classified the species. Among them, 13 lines of 2 species (G. uralensis Fisch, G. glabra L.) were selected from 2015 to 2016. We evaluated the aerial parts, underground parts, yield and glycyrrhizin content of 13 line of licorice grown one year. Plant length and stem diameter of GLY2014 - 002 was high and thick on the aerial parts. GLY2013 - 005 had many pods. GLY2014-006 had many adventitious roots like taproot (3 ea/plant) and high of root weight (170 g/plant), GLY2013 - 005, GLY2014 - 006 had high glycyrrhizin content (2.1%) in the underground parts. Conclusion : As a result of growth characteristics and glycyrrhizin analysis, GLY2013 - 002, GLY2013 - 005, GLY2013 - 007, GLY2014 - 002, GLY2014 - 006, and GLY2014 - 007 were evaluated as good resources. Therefore of six lines will be used to test productivity and conduct regional testing for developing licorice variety.

Background : Recently, there is an urgent demand for development of new varieties with enhanced resistance to various biotic/abiotic stresses. One generation of ginseng is four years, so it takes a long time to breed. To increase the efficiency of the ginseng breeding and save time and effort, it is necessary to build a ginseng core collection. This study examined the major growth characteristics of genetic sources for the establishment of core collection. Methods and Results : As plant materials, 187 ginseng lines collected in 2003 were used in this study. Ginseng Seeds were harvested at the end of July 2014, sown in mid-November, and cultivated in 2015 for one year in the field and then transplanted into the main field in 2016. All phenotypes including agronomic characteristics, seed yield, and physiological response to biotic/abiotic stresses were investigated according to the ginseng GAP and UPOV guidelines. The stem colors of the collected ginseng germplasm were classified as the five classes; light green, green, light purple, purple and dark purple, but most of them were purple or dark purple. The locations of distribution of anthocyanin coloration in stem were classified into the four classes; proximal end, proximal end and petiole part, the entire stem and the stem with purple not revealed, but most of them were petiole part or the entire stem. The shapes of leaflet were classified the four classes; the long elliptic, elliptic, slender type, and spatulate type. Most of surveyed lines were elliptic type. When the leaflet of ginseng was laterally cut, the shape was classified into the three types; concave type, plane type and convex type. Most of surveyed lines were red berry color. Conclusion : The genetic pool of ginseng is known to be narrow. The results of this study showed similar characteristics among the ginseng fields collected. In the future, we will carry out the survey about quantitative characteristics and correlations of ginseng genetic resources for establishment of Korean ginseng core collection.

Background : The study is investigated the development of high-quality standard variety, character of gene resources, growth and yield production to collecting varieties in Agastache rugosa O. Kuntze containing antioxidant agriculturally. Methods and Results : Character of gene resources investigated collecting 16 varieties (Jeonnam Naju 1, 2, 3 (NJ), Gwangyang 1, 2 (GY), Sooncheon 4 (SC), Boseong 1, 2 (BS), Yeosu (YS), Jangheung (JH), Jindo (JD), Gyeongnam Hamyang (HY), Gyeongbuk Bonghwa (BH), Chungbuk Eumseong (ES), Cheongju (CJ), Gangwon Cheolwon (CW). Method of cultivation were conducted under sowing dates of plant a seedling were April 24. Transplanting with Agastache rugosa O.K. seeds in June 27 by growing pot seedling for 60 days in 2017. Fertilization application were standard application (N-P-K-Compost applied at 12-16-10-600 ㎏․10a−1). N-K applied at 60% of basal fertilizer, 40% of top dressing were two times (N-K) application. Planting density were spaced 30 ㎝ apart in rows 20 ㎝ apart with with non-woven fabric mulching cultivation. Plot design randomized block 3 repetition. Conclusion : Endemic characteristics of gene resources to collecting variety in leaf color (light green), leaf pliable (softness) appeared 8 lineage, leaf color (deep green), the contrary leaf pliable (roughness) showed 8 lineage. Flower color bloomed in light purple 8 lineage flower deep purple color come into 8 lineage. Also, the period of maturity ripened Sept. 27 - 31. on 8 lineage (BS2, GY1, JD, HY etc.). 3 lineage (YS, CJ, CW) attained to maturity on Oct. 14 - 16. Growth of aerial part increased on long length, large width of leaf, heavy dried weight of leaf and weight of seeds 10 lineage SC4, GY1, 2, NJ2, 3, BS1, 2, CJ, ES, BH etc. but short length, small width of leaf, declined in light dried weight of leaf and weight of seeds 2 lineage YS, CW.

Background : Sorghum (Sorghum bicolor) is a perennial plant of the Gramineae, followed by rice, wheat, corn and barley, the fifth major food crop in the world. Sorghum is mostly used in Korea for cereals using seeds or for making food directly. However, sorghum is not directly used for food in foreign countries, but plays an important role in the development of cosmetic and pharmaceutical materials and as a bio-energy crop. Most of the sorghum cultivated in Korea does not use the remainder after harvesting seeds, so research on other uses is needed. Therefore, we carried out this experiment in order to identify the characteristics of domestic genetic resources and to select the genetic resources with excellent growth. Methods and Results : Samples were tested for their growth characteristics by the Agricultural Genetic Resources of National Institute of Agricultural Sciences. Growth characteristics of heading, total length, stem length, stem width, leaf length, leaf width, length of ears and width of ears were investigated. After 10 weeks of sowing, 45% of the outbreaks occurred and showed a quick heading. The total length of the sorghum ranged from 65.7 ㎝ to 341.0 ㎝, and the stem width ranged from 0.2 ㎝ to 2.6 ㎝. Leaf length ranged from 33.3 ㎝ to 92.4 ㎝ and leaf width ranged from 3.8 ㎝ to 12.2 ㎝. The length of the ears was in the range of 8.0 ㎝ - 52.7 ㎝, and the width of the ears was in the range of 5.2 ㎝ - 27.0 ㎝. K131301, K178840, K258654 and K255198, which are expected to bear a large number of seeds with high biomass content and large size of leaves and plants, were selected. Conclusion : Through this study, we can understand the growth characteristics of the sorghum in Korea and select excellent genetic resources.

Environmental pollution is the major problem associated with rapid industrialization, urbanization and rise in living standards of people. In order to reduce environmental pollution, therefore, shielding materials using industrial waste resources were developed and the radiation shielding performance was evaluated.

Background: This study was conducted to acquire basic information on the phenotypic and genotypic characteristics of the germplasm of Panax ginseng C. A. Meyer collected from China and Korea, and identify the variations that can be utilized in ginseng breeding programs. Methods and Results: Quantitative parameters were evaluated, and used to compare and analyze on genetic polymorphisms in the germplasm. The genetic characteristics and classifications were compared and analyzed for each character. Stem length followed a normal frequency distribution ranging from 15.5 ㎝ to 40.5 ㎝, with showing approximately 40% having a stem length of 20 - 30 ㎜. Stem diameters ranged from 2.7 ㎜ to 11.3 ㎜. Stem number per plant ranged from 1 to 3; approximately 50% had a single stem, and 45% had two stems. A non-normal frequency distribution was observed for petiole number, with approximately 60% of the germplasm having 3 - 5 petioles. Petiole length exhibited a normal frequency distribution, raging from 4.5 to 10.6. Petiole angle in the germplasm ranged from 28° to 89° and seedstalk length ranged from 5.6 ㎝ to 27.3 ㎝. Conclusions: The genetic polymorphisms identified by complete linkage clustering based on the quantitative characteristics of Panax ginseng C. A. Meyer collected from Korea and China were classified to 6 groups, namely I, II, III, IV, V, and VI with frequencies of 6.7%, 20.0%, 31.7%, 8.3%, 6.7%, and 26.7%, respectively.

한국은 광물자원의 90 %, 에너지의 97 % 이상을 수입하는 국가이며, 매립되거나 단순소각 되는 폐기물 중에 회수 가능한 물질이 56 % 포함되어 있다. 2015년 발생 폐기물의 매립처분 비율은 9.2 %(38,308 ton/day)이다. 이중 사업장배출시설계폐기물이 약 62 %(23,577 ton/day)로 가장 높은 비율을 차지하고 있다. 사업장배출시설계폐기물에서 무기성폐기물의 매립처리량은 소각재 4,283 ton/day, 연소재 3,910 ton/day, 폐주물사류 939 ton/day 순으로 높게 나타났다. 무기성폐기물 중 열적처리 잔재물류의 매립량은 10,637 ton/day로 사업장배출시설계폐기물 매립량의 45.1 %을 차지하고 있는 것으로 조사되었다. 본 연구에서는 무기성폐기물의 매립억제 및 재활용 가능성을 알아보기 위해 강열감량, 총유기탄소, XRF 등의 분석을 통하여 화학적 조성 및 물질특성을 살펴보았다. 예로, 재생연을 생산하는 S사 광재류의 경우 Fe(43.0 %), S(23.1 %), Na(19.4 %) 함유량이 주로 높게 나타났다. 망간 합금철을 생산하는 D사의 광재는 Mn(29.9 %), Si(23.4 %), Ca(23.1 %)의 높은 함량을 나타내었다. M사 폐주물사의 경우 Si (74.2 %)로 대부분이 Si로 형성되어 있음을 확인할 수 있었다.

This experiment was conducted to investigate the aspect of tiller occurrence, growth and yield of sorghum according to planting distance and sowing date. The object of this experiment is to get basic data to inhibit non-productive tillers uneconomical and cumbersome for mechanical harvesting. Also another object was to evaluate optimum planting distance and sowing date in central district area. Total number of tillers was more in 80 ㎝ ridge than 60 ㎝ ridge and it was increased as the planting distance was wider from 15 ㎝ to 30 ㎝ on the each ridge. Ratio of effective tillers was higher in 60 ㎝ ridge than 80 ㎝ ridge and it was decreased as planting distance was wider from 15 ㎝ to 30 ㎝. The lower the planting distance, the more increased total number of tillers, whereas effective tillers was decreased as planting distance was high. Average of total number of tillers of three varieties was higher in sowing date of May 2 (1st sowing date), whereas ratio of effective tillers was the highest in sowing date of May 23 (2nd sowing date). Hwanggeumchal showed the highest total number of tillers (1.2 tillers), while Moktaksusu had the lowest total number of tillers (0.8 tillers) among three varieties. There were no significant difference between planting distance and days to heading and ripening date from seeding. Culm length increased as planting distance was high, but ear length, grains per ear and 1000 grain weight were decreased on the other hand. The highest yield of sorghum per 10a was obtained from 60×20 ㎝ planting distance among 6 planting distances.

Background : The study was conducted in response to climate change, to develop cultivation techniques by introducing a new crop income. Therefore, we want to collect the Coriander sativum genetic resources that are grown in different regions of the korea and other countries. The C. sativum were strengthens the stomach to say "housil" in Herbal and prosper digestion. Recently, several nations, including Vietnam and Thail and increased in Korea. C. sativum increased eating enjoy food. The people who like C. sativum in Korea is estimated to be 1.5 million. Methods and Result : First was C. sativum 93 species in National agrobiodiversity Center RDA lotting-out on May 12, 2016 for the study. C. sativum germplasm received lotting-out of over thousand grain 15g 28 kinds of seeds, 10∼15g was 23 species, 10g less than 42 species. 32 kinds of fruit color is white, black, 30 species, the gray was 31 species. Conclusion : C. sativum lotting-out was conducted by resource for two weeks from germination test from May 31 to June 13. The results were as follows: C. sativum port germination 86∼100% germination was 43 stocks, 40∼60% of 23 stocks, 5 stocks of 20∼ 40%. The 22 stocks were not germinate paper. C. sativum resources per port germination test was conducted 13 days from 29 days July until August 9th. As a result, more than 90% of the port germination is 62 stocks, not more than 80∼90%, 50∼80% 18 stocks of germination was 5 stocks. At this time, no germination was 8 stocks. Therefore, C. sativum germination test is a port germination test obtained a more than satisfactory result.