음식물류폐기물의 퇴비화는 경제적인 자원화 방법이지만, 염분함량이 높아 토양에 투입될 경우 염류집적으로 인한 작물 생육 피해가 우려된다. 바이오차는 양이온교환능력이 높은 물질로 염분피해가 우려되는 토양에 적용 시 Na+이온을 흡착하여 작물생육 피해를 줄여줄 것으로 생각된다. 이에 본 연구는 바이오차와 혼합하여 제조한 음식물류폐기물 혼합 펠렛 퇴비(이하, 음폐혼합펠렛퇴비) 사용 시 배추 생육과 토양에 미치는 영향을 평가하고 이에 따른 무기질비료 절감 효과를 알아보고자 하였다. 처리구는 무비구(NF), 무기질비료 반량(NPK0.5), 무기질비료(NPK), 무기질비료+ 음폐퇴비(FWC), 무기질비료+음폐혼합펠렛퇴비(FWCB)이다. 배추 생육조사 결과, FWCB처리구에서 엽장, 엽폭, 구고 및 구폭 모두 가장 높았다. 수량조사 결과 8,300 kg 10a-1로 가장 높게 나타났으며, NPK처리구에 비해 무기질비료를 50% 사용하였으나 수량은 13.6% 높게 나타났다. 토양화학 성 결과 FWCB에서 EC는 0.59 ds m-1, 교환성 Na은 0.35 cmolc kg-1으로 다른처리구보다 높게 나타났으나 적정범위 이내였다. 본 연구결과, 음폐혼합펠릿퇴비의 사용은 작물의 양분 및 유기물공급 뿐만 아니라 바이오차로 인한 양이온 고정 효과로 작물 생산성을 향상시키는 것으로 생각되며, 비료성분의 공급원으로써 배추의 생육 및 생산성을 증진할 수 있는 기술로 활용이 가능할 것으로 판단된다.

Bio-efficacies of two different types of fungicides, Diniconazole and Paclobutrazol with their effects as plant g rowth regulators f or Kimchi Cab bage were e valuated o n February 4 to A pril 13, 2024 in Los Baños, Laguna, Philippines. The experiment was done during the off-season planting of Kimchi Cabbage in the country. Yield and other horticultural characteristics were observed for seven different groups: group 1, untreated control; group 2, recommended rate of granular fertilizer (RR-G); group 3, recommended rate of granular fertilizer plus recommended rate of Diniconazole; group 4, recommended r ate of D iniconazole (alone); g roup 5 , recommended rate o f Paclob utrazol (RR-PBZ), group 6, RR-G plus RR-PBZ; and group 7, RR-G plus ½ RR-PBZ. Results showed that combination of recommended rate of granular fertilizer plus the full recommendation of Paclobutrazol (group 6) resulted in a significantly higher yield of 64.9 tons/ha than other groups with yields ranging from 23.3 to 55.3 tons/ha. Such significantly higher yield in group 6 was also attributed to the number of leaves produced by plants at the time of harvest. Regarding effects of two chemical treatments, the combination of Diniconazole a nd P aclob utrazol a s recommended granular f ertilizers h elped in t he heading of K imchi Cabbage during hot conditions with an average temperature of 32-35°C from March to April at the heading stage plus a f actor of b eing planted a t a lowland area i n the country. T he a dvantage o f Paclobutrazol aside from yield is its availability in the country as compared to Diniconazole (Binnari).

Drought is one of the environmental factors inhibiting plant productivity and growth, leading to oxidative damage. This study aims to identify the role of sodium hydrosulfide (NaHS) as a hydrogen sulfide (H2S) donor in drought stress tolerance in Brassica napus. Drought-induced stress symptoms appeared eight days after treatment, showing wilted leaves and a significant reduction of leaf water potential. Drought-induced increase of lipid peroxidation was significantly reduced by NaHS application. NaHS-treated plants mitigated stress symptoms under drought conditions by reducing hydrogen peroxide (H2O2) content, confirmed with H2O2 localization in situ. Furthermore, NaHS promotes photosynthetic activity by maintaining chlorophyll and carotenoid content, thereby supporting plant growth under drought conditions. Pyrroline-5-carboxylate and proline contents were significantly increased by drought but further enhanced by NaHS treatment, indicating the important roles of proline accumulation in drought stress tolerance. In conclusion, this study provides valuable insight into the roles of NaHS in alleviating drought stress by reducing oxidative stress and promoting proline accumulation. Therefore, NaHS may serve as an effective strategy to enhance crop production under drought-stress conditions.

Background: Brassica oleracea var. italica (broccoli), a rich source of antioxidants, can prevent various diseases and improve human health. In this study, we investigated the antioxidative effects of broccoli sprout extract on oxidative stress induced by lipopolysaccharide and cisplatin in cell and organ tissue models. Methods: Antioxidative effect of BSE was evaluated using DPPH and ABTS in RAW 364.7 cells, and effects of BSE on testes were investigated using Cisplatin-induced testicular damage model with an in vitro organ culture system. Results: The DPPH assay showed that the antioxidant activity of the alcoholic broccoli sprout extract was higher than that of the water extract. Additionally, the expression levels of antioxidation-related genes, Nrf2 , Gsr , HO-1, and catalase , were significantly increased in broccoli sprout extract-treated RAW 264.7 cells, and the extract suppressed lipopolysaccharide-induced mitochondrial dysfunction. Based on the results in the RAW 264.7 cell culture, the antioxidative effects of the extracts were investigated in a mouse testis fragment culture. The expression of Nrf2 , HO-1 , and Ddx4 was clearly decreased in cisplatin-treated mouse testis fragments and not in both broccoli sprout extract- and cisplatin-treated mouse testis fragments. In addition, the oxidative marker O-HdG was strongly detected in cisplatin-treated mouse testis fragments, and these signals were reduced by broccoli sprout extract treatment. Conclusions: The results of this study show that broccoli sprout extracts could serve as potential nutraceutical agents as they possess antioxidant effects in the testes.

Herbicide-resistant transgenic rapeseed (TG rapeseed) was developed by inserting phosphinothricin acetyltransferase (PAT, bar), a modified gene from the soil bacterium Streptomyces hygroscopicus, into the genome of a conventional variety of rapeseed (Youngsan). The TG rapeseed used for the test was confirmed to express the PAT gene by polymerase chain reaction (PCR) and immunostrip. Feeding tests were conducted with Cyprinus carpio to evaluate the environmental risk of TG rapeseed, including the herbicide resistant gene. C. carpio was fed 100% ground rape suspension, TG rapeseed, or non-genetically modified (GM) counterpart rapeseed (Youngsan). As a result, the feeding test showed no significant differences in the cumulative immobility or abnormal response between C. carpio samples fed on TG rapeseed and non-GM counterpart rapeseed. The 48 h-LC50 values of the TG rapeseed and the non-GM counterpart rapeseed were 3,376 mg/L (95 % confidence limits: 3,169 - 3,596 mg/L) and 2,682 mg/L (95 % confidence limits: 2,267 – 3,123 mg/L), respectively. The rape NOEC (no observed effect concentration) value for C. carpio was suggested to be 625 mg/L. Based on these results, there was no significant difference in the toxicity for non-target organisms (C. carpio) between the TG rapeseed and non- GM counterparts.

The effects of exogenous sodium nitroprusside (SNP, nitric oxide donor) on the growth, yield, photosynthetic characteristics, and antioxidant enzyme activity of kimchi cabbage (Brassica rapa L. subsp. pekinensis (Lour.) Hanelt) was studied under the low temperature conditions. Kimchi cabbages were treated with SNP of three concentrations (7.5, 15, 30 mg·L-1) for three times at four-day intervals and exposed to low temperature (16/7°C) stress for seven days. SNP treatment induced increases of net photosynthetic rate (Pn), stomatal conductance (Gs), intracellular CO2 concentration (Ci) and transpiration rate (Tr) under the stress condition with the highest level after the third treatment. The contents of malondialdehyde (MDA) and H2O2 were significantly lower in the treatment of SNP compared to the non-treated control. The activity of ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD), increased in treated plants by up to 38, 187, 24 and 175%, respectively compared to the non-treated control. SNP-treated and untreated plants had similar growth characteristics. Compared to the control group, SNP-treatment increased fresh weight and leaf area by 5%. Overall, our findings suggest that the application of sodium nitroprusside to the leaves contributes to reducing physiological damage and enhancing the activities of antioxidant enzymes, thereby improving low temperature stress tolerance in kimchi cabbage.

This study was conducted to provide basic data on the antioxidant activity, inhibition of adipocyte differentiation and reactive oxygen species (ROS) production of a mixture of Brassica juncea extract (BJE) and fermented black rice fraction (BRF). We investigated the total phenol content, total flavonoid content, antioxidant effects (DPPH radical scavenging, ABTS radical scavenging, reducing power, FRAP and ORAC assay) and anti-obesity activity of the mixture in 3T3-L1 cells. Our results showed that the total phenol and flavonoid content increased with increasing BRF mixture ratio. The antioxidant activity increased as the BRF mixture ratio increased. In addition, BJE and BRF mixtures did not show any cytotoxicity during the 3T3-L1 differentiation period. During adipocyte differentiation, BJE and BRF mixtures significantly inhibited lipid accumulation and ROS production compared to the control group. These results warrant further experiments to develop an anti-obesity functional food using a mixture of BJE and BRF.

Ammonium (NH4 +) serves as a nitrogen source, but its elevated levels can hinder plant growth and production. Excess NH4 + with α-ketoglutarate is assimilated into glutamate, a precursor of proline and glutathione (GSH). This study aimed to investigate the effects of excessive NH4 + on the regulation of proline and GSH synthesis. Detached leaves from oilseed rape (Brassica napus L.) were fed with 0, 50, 100, 500, and 1000 mM NH4Cl for 16 h. As the NH4 + concentrations increased, the leaves exhibited progressive wilting and yellowing. Furthermore, total carotenoid and chlorophyll concentrations declined in response to all NH4 + treatments, with the lowest levels observed in 1000 mM NH4 + treatment. Hydrogen peroxide (H2O2) concentration showed a minor increase at low NH4 + concentration (50 and 100 mM) treatments but a significant increase at high NH4 + (500 and 1000 mM), which was consistent with the localization of H2O2. Amino acid concentrations increased with increasing in NH4 + concentration, while the protein concentration displayed the opposite trend. Proline and cysteine concentrations exhibited a gradual increase in response to increasing NH4 + concentrations. However, GSH concentrations rose only in the 50 mM NH4 + treatment and decreased in the 500 and 1000 mM NH4 + treatments. These results indicate that excessive NH4 + is primarily assimilated into proline, while GSH synthesis is adversely affected.

The germination process is critical for plant growth and development and it is largely affected by environmental stress, especially salinity. Recently, hydrogen sulfide (H2S) is well known to act as a signaling molecule in a defense mechanism against stress conditions but poorly understood regulating seed germination. In this study, the effects of NaHS (the H2S donor) pretreatment on various biochemical (hydrogen peroxide (H2O2) content and amylase and protease activity) and physiological properties (germination rate) during seed germination of oilseed rape (Brassica napus L. cv. Mosa) were examined under salt stress. The seed germination and seedling growth of oilseed rape were inhibited by NaCl treatment but it was alleviated by NaHS pretreatment. The NaCl treatment increased H2O2 content leading to oxidative stress, but NaHS pre-treatments maintained much lower levels of H2O2 in germinating seeds under salt stress. Amylase activity, a starch degradation enzyme, significantly increased over 2-fold in control, NaHS pretreatment, and NaHS pretreatment under NaCl during seed germination compared to NaCl treatment. Protease activity was highly induced in NaHS-pretreated seeds compared to NaCl treatment, accompanied by a decrease in protein content. These results indicate that NaHS pretreatment could improve seed germination under salt stress conditions by decreasing H2O2 accumulation and activating the degradation of protein and starch to support seedling growth.

Kale (Brassica oleracea var. acephala) is one of the most frequently consumed leafy vegetables globally, as it contains numerous nutrients; essential amino acids, phenolics, vitamins, and minerals, and is particularly rich in glucosinolates. However, the differences in the biosynthesis of glucosinolates and related gene expression among kale cultivars has been poorly reported. In this study, we investigated glucosinolates profile and content in three different kale cultivars, including green (‘Man-Choo’ and ‘Mat-Jjang’) and red kale (‘Red-Curled’) cultivars grown in a vertical farm, using transcriptomic and metabolomic analyses. The growth and development of the green kale cultivars were higher than those of the red kale cultivar at 6 weeks after cultivation. High-performance liquid chromatography (HPLC) analysis revealed five glucosinolates in the ‘Man-Choo’ cultivar, and four glucosinolates in the ‘Mat-Jjang’ and ‘Red-Curled’ cultivars. Glucobrassicin was the most predominant glucosinolate followed by gluconastrutiin in all the cultivars. In contrast, other glucosinolates were highly dependent to the genotypes. The highest total glucosinolates was found in the ‘Red-Curled’ cultivar, which followed by ‘Man-Choo’ and ‘Mat-Jjang’. Based on transcriptome analysis, eight genes were involved in glucosinolate biosynthesis. The overall results suggest that the glucosinolate content and accumulation patterns differ according to the kale cultivar and differential expression of glucosinolate biosynthetic genes.

Chinese cabbage or pakchoi (Brassica Rapa subsp. Chinensis) is one of the most popular vegetables in Korea. It is grown and consumed throughout the year because it has many benefits. This study reports on the growth performance of pakchoi grown in the hydroponic system using a Food Juke Box (FJB) under different light compositions. The chlorophyll content (CC), root fresh weight (RFW), and shoot fresh weight (SFW) were measured. Further, various vegetation indices (VIs), such as modified chlorophyll absorption in reflectance index (MCARI1), normalized difference vegetation index (NDVI), optimized soil-adjusted vegetation index (OSAVI), and structure insensitive pigment index (SIPI), were employed to predict SFW. The study results indicated a significant increase in the CC of pakchoi with different light treatments compared with the control. The CC was highest under treatment with 70% white light, 20% blue light, and 10% red light (T1) than under treatment with 50% white light, 30% blue light, and 20% red light (T2). All used VIs showed significant differences with different light combination treatments. There was no significant change in RFW and SFW between the control and T1. However, a significant reduction in RFW and SFW was observed in T2 compared with the control. A comparison between T1 and T2 demonstrated that RFW and SFW were increased by 23% and 25%, respectively, compared with those in T2. The correlation result showed that SFW had a significant positive correlation with RFW (0.76***). Further, a significant negative correlation was observed with OSAVI (−0.25*), MCARI1 (−0.54***), and CC (−0.19*). In conclusion, our findings implied that different light combinations in pakchoi affected the photosynthetic pigments although they did not improve SFW. This research will pave the way for pakchoi production in hydroponics using smart farming FJB. This will further promote plant development, especially for domestic consumption, and help fulfill the growing demand for leafy vegetables.

This study evaluates the gastroprotective effect of cabbage extract with sulforaphane content of 5.19 mg/L and Smethylmethionine content of 469.28 μg/L. In vitro, the lipopolysaccharide (LPS)-treated group had an increased NO activity compared to the normal group, and the concentration of NO was reduced when the cabbage extract was treated in the dose manner. The level of IL-6 induced by LPS was dose-dependently reduced when the extract was treated. The cabbage extract concentration was orally administered in rats at 5.75 mg/kg, 11.5 mg/kg, and 23 mg/ kg, and the inhibitory effect on gastric damage by HCl-ethanol was observed. Histological analysis exhibited mucosal erosion in the gastritis model compared to the normal group, while the ameliorating effect of the generated erosion was observed in the cabbage-treated group. The histamine concentration was significantly increased in the gastritis-induced animal model, and the histamine concentration was decreased in the 23 mg/L-treated group of cabbage extract. In conclusion, the results of this study suggest that cabbage extract not only down-regulates cytokines in vitro, but is also directly involved in histamine secretion in an animal model of gastritis; therefore, cabbage extract can help inhibit gastrointestinal disorders by improving the protective barrier.

본 연구는 십자화과 채소인 갓(Brassica juncea) 추출물 을 이용하여 지표성분인 sinigrin의 함량을 분석하고, 내분비계 교란물질 환경호르몬인 비스페놀 A (BPA)로 분화를 유도한 3T3-L1 전구지방세포에서 갓 추출물과 sinigrin 처 리에 대한 지방세포 분화 및 활성산소종(ROS) 생성 억제, 지방 생성 전사인자(PPARγ, C/EBPα, aP2)의 단백질 발현 감소 효능을 평가하였다. 연구 결과에 따르면 HPLC를 이용하여 측정한 갓 추출물 중 sinigrin의 함량은 21.27±0.2 mg/g 인 것으로 나타났다. BPA로 유도된 3T3-L1 전구지방세포에 서 XTT assay 결과 sinigrin 180 μM 및 갓 추출물 300 μg/ mL 농도에서 세포 독성을 보이지 않았으며, 지방세포 분화과정 중 세포 내 지방 축적량과 ROS 생성량을 비교하였을 때 갓과 sinigrin을 처리한 지방세포의 경우 지방축 적량 및 ROS 생성량 모두 유의적으로 감소시키는 것으로 나타났다. 또한, 갓 추출물 및 sinigrin을 처리하였을 때 지 방세포 분화를 조절하는 전사인자 PPARγ, C/EBPα 및 aP2 의 발현이 억제됨을 확인하였다. 이 결과를 통해 갓은 환 경호르몬 비스페놀 A로 유도된 지방세포 내 지방 축적 억제와 더불어 ROS 저감에 효과적으로 작용함을 확인하였다. 향후 sinigrin을 함유한 갓은 BPA로 인한 지질 대사 장애를 예방하는 천연물 유래 기능성 식품 소재로 활용할 가능성이 높은 것으로 기대되며, 추가로 임상 연구 및 작용기전 입증을 위한 in vivo 모델에서의 후속 연구가 진 행되어야 할 것으로 사료된다.

In recent years, there has been an increase in the morbidity of gastritis in Korea due to lifestyle factors mostly changes in eating habits and stress. Gastritis is more likely to progress to gastric cancer, and therefore it is important to prevent and manage gastritis through lifestyle adjustment and treatment at an early stage. In this study, cabbage, which was found to be effective in gastritis, was mixed and fermented with other crucifer plants such as kale and broccoli to evaluate the overall efficacy of fermented brassica puree on alcoholic acute gastritis. Based on our results, fermented brassica puree alleviated gastric injury induced by 150 mM HCl/60% ethanol. In addition, it was confirmed that PGE2, a gastric mucosal protective factor, was increased, and other positive effects such as an increase of MUC1 and regulation of PKC were observed. The results of this study suggest that fermented brassica puree can relieve acute alcoholic gastritis by regulating PGE and the expression of MUC1, a gene related to mucus secretion, and activating PKC, which is related to mucosal cell activity.

This study was carried out to select the suitable and high leaf yielding broadleaf mustard genotypes for the mid-hill conditions of Nepal. We evaluated seven germplasms of broadleaf mustard (Brassica juncea var. rugosa (L.) Czern.) namely, ‘HRDBLM001’, ‘HRDBLM004’, ‘HRDBLM007’, ‘HRDBLM009’, ‘HRDBLM010’, ‘Marpha Chauda Pat’, and ‘Khumal Chauda Pat’ in the field of the National Horticulture Research Centre, Khumaltar under Nepal Agricultural Research Council during the winter season of two consecutive years, 2019 and 2020. The germplasms were collected from the local diversity centres in the country. The experiment was laid out in the Randomized Complete Block Design (RCBD) in three replications per treatment. Seeding was done in September, and 28-day-old seedlings were transplanted in the field. The results obtained from the two consecutive seasons revealed that plant vigour was found to be greater in ‘HRDBLM010’ and ‘Khumal Chauda Pat’ (4.7 in the 1 to 5 scale score). The highest leaf weight (65.1 g) was found in ‘HRDBLM010’, followed by ‘HRDBLM007’ (45.6 g) and ‘Khumal Chauda Pat’ (40.9 g). The highest number of leaves was produced by ‘HRDBLM004’ (50 leaves per plant), while leaf weight per plant was the highest in ‘HRDBLM010’ (691.1 g per plant) followed by ‘HRDBLM004’ (535.4 g per plant). Genotype ‘HRDBLM010' produced the highest leaf yield (54.52 MT ha-1), followed by 'HRDBLM004' (44.28 MT ha-1) and ‘HRDBLM007’ (41.65 MT ha-1). Thus, these three germplasms showed superiority among the seven tested germplasms and they could be recommended for cultivation in the mid-hill conditions of Nepal.

본 연구는 지역별로 수집한 유채 균핵병 균주에 대해 등록 된 3종의 약제를 사용하여 저항성 검정을 실시하였고, 저항성 발생 가능성이 있는 약제의 작용 기작과 관련한 유전자를 분석하여 변이 유무를 확인하였다. 1. Carbendazim-diethofencarb 약제배지의 경우, 0.1 ppm 농도에서 균사 생장 억제율은 13.3~41.9% 범위로 나타났으며, 1 ppm 이상의 농도에서는 모든 균주에서 96.1% 억제율을 보여 균주의 저항성이 확인되지 않았다. 2. Fludioxonil 약제배지는 0.1 ppm 농도에서 균사의 생장이 94.2% 이상 억제되었으며, 1 ppm 농도에서부터 100%의 억제 율을 보여 가장 약제 효과가 우수한 것으로 나타나 수집한 모든 균주에서 약제의 감수성을 확인하였다. 3. Boscalid 약제배지는 앞선 2종의 약제에 비해 균주의 균사 생장 억제가 뚜렷하지 않았다. 특히 10 ppm 농도에서 무안 수집 균주는 93.9%, 나주 수집 균주는 79.3%로 지역 간 차이가 있었으며, 1000 ppm의 높은 약제 농도에서도 균사의 생장을 100%까지 억제하지 못해 약제에 대한 균주의 저항성 발생 가능성을 추측하였다. 4. 3종의 시험 약제 농도별 균핵병 균주의 균사 생장을 50% 억제하는 농도(EC50)를 분석한 결과, Fludioxonil, Carbendazim-diethofencarb, Boscalid 약제순이었으며, 그 값은 각각 0.06, 0.16, 0.43 ppm으로 나타났다. 5. 또한, 3종의 시험 약제 농도별 발생한 균주의 균핵 형성 능력은 1 ppm 농도에서 Carbendazim-diethofencarb는 5.6개, Fludioxonil은 0개로 나타난 반면, Boscalid는 최대 11.3개의 균핵이 형성되어 차이를 확인할 수 있었다. 6. Boscalid 약제에 대한 균주의 저항성을 확인하기 위해 해당 약제의 작용 기작인 SDHI와 관련된 유전자 SdhB를 염기 서열 분석하였다. 염기서열 분석 결과 무안 및 부산에서 수집 한 균주의 경우 SdhB 표준 염기서열과 일치하여 감수성이었으나, 나주, 당진, 제주, 영암에서 수집한 균주는 32번째 염기 가 C→T로 치환되어 GCA(Alanine)→GTA(Valine) 점 돌연변이를 확인하였다.