초 록 1. 인삼묘포에 Captan, Difolatan, Zineb, Maneb와 PCNB를 일주일 간격으로 토양관주 하였다. 처리하기전과 2회 및 4회처리후에 각각 Rhizoctonia solani, Pythium debaryanum, Fusarium 그리고 Trichoderma 균총의 숫적 (수적)변화를 Boosalis의 특별잔사법에 의해서 조사하였다. 우리나라에서 인삼(Panax ginseng)의 모잘록병균으로서 Pythium debaryanum을 처음으로 기술하였다. 2. Rhizoctonia solani, Pythium debaryanum의 수는 토양관주에 관계없이 시일이 경과함에 따라서 점차로 감소하였고 Fusarium 및 Trichoderma는 오히려 증가하였다. Rhizoctonia solani의 수는 PCNB에 의해서 현저하게 감소하였고 다른 약제에 의해서도 대조구 보다 감소하였다. 3, Pythium debaryenum은 Zineb, Maneb, Captan, Difolatan의 순으로 고 수가 증가하는 반면 Fusarium은 일정한 경향을 볼 수 없었다. Tichroderma를 제외한 모든 균의 숫적 변화는 시일의 경과에 대하여 수준에서 유의성이 있었다. 4. 토양관주에 의한 약해는 Maneb, Zineb, Captan 구에서 나타났고 묘삼 뿌리의 생체 무게는 Difolatan, PCNB, Maneb구에서 대조구 보다 증가하였다.

Background : The cultivation method of ginseng (Panax ginseng C. A. Meyer) has been introduced not only in the existing shading facilities but also in the cultivation method using the rain shelter house. In addition, direct seedling cultivation of ginseng is increasing in order to save labor. Ginseng cultivation area in the northern area is increasing with the disappearance of first planted field. Proper shading method in the northern ares is needed to grow direct grown ginseng using rain forest house. Methods and Results : Shading method using rain shelter house was Polyethylene film with layered polyethylene net 75%, blue-white duplicated PE film 85%, blue-white duplicated PE film 90%. Plant materials is 5-years-old Ginseng using direct seedling cultivation. In order to select proper shading materials of the rain shelter house in northern area, we investigated photosynthetic properties and growth of 5-years-old ginseng in rain shelter house. The temperature and humidity were slightly different between the traditional conventional shade facility and the rain shelter house, but the light intensity of the shading facility tended to decrease with the passage of time. The difference in shading method in rain shelter house was the highest at polyethylene film with layered polyethylene net 75%. As a result of the growth characteristics of the top part, 90% of blue-green double-sided film showed the greatest growth with 89.8 ± 1.5. The difference in top part between rain shelter house and shading facility is about 23 ㎝. Conclusion : Although the above growth characteristics of the ginseng shows this tendency, the root growth characteristics of the ginseng must be further observed.

Background : Multi-stage system were used for development of stable ginseng seedling production. Bed-soil for the production of ginseng seedling in the multi-stage was similar to the conventional bed-soil but the weight of the bed-soil was high and the efficiency of operation in the multi-stage was lowered. In this study, the yield and quality of ginseng seedling was investigated by commercial lightweight bed-soil in the multi-stage facilities, and the possibility of application of lightweight bed-soil. Methods and Results : This study was carried out by a 3-stage cultivation bed using a 50 ㎜ sandwich panel in a house shaded with 85% light-shielding net. The width of the cultivating bed was 90 ㎝, the height was 30 ㎝, and the height of each stage was 50 ㎝. In the first and second stages, the amount of light was insufficient, so two rows of fluorescent lamps were installed and the third stage was used natural light. Ginseng seeds were sown on the cultivating bed in November 2016, and ginseng seeds (native species) were sown with a density of 3 × 3 ㎝. The chemical properties of lightweight bed-soil were pH 5.11, and EC 0.76 dS/m. It was suitable for ginseng seedling cultivation. The bulk density was 0.21 Mg/㎥. Among the growth characteristics of the ginseng seedlings, the root length was the longest as 17.0 ㎝ in the conventional cultivation, and the second stage was the longest at 14.8 ㎝ in the multi-stage facility. The root diameter in the multi-stage system was 0.2 - 0.4 ㎜ thicker than the conventional one. Root weights of lightweight bed-soil were similar to those of conventional cultivation. The yield of ginseng seedlings in the 1 st, 2 nd and 3 rd stage was 721.3 g, 692.0 g, and 394.7 g/1.62 ㎡ respectively. Conclusion : In the production of ginseng seedling using multi-stage facilities, the commercial bed-soil was better than the conventional bed-soil (light, workability). The differences in yields in the multi-stage facilities can be overcome if the growth management such as moisture management is more systematic. If we develop the technology to reuse the bed-soil after harvesting the ginseng seedling, it will be economical and able to supply to farmers.

Background : Panax ginseng C.A. Meyer belong to Panax species Araliaceae and a perennial plants. It grows in Far East Asia such as the Maritime province in Russia, the Manchuria reg ion of China, and the Korean peninsula. Panax ginseng C.A. Meyer is cultivated by seed sow ing or transplanting-culture, but seedling standing rate is as low as 50%. Therefore, it is impo rtant to increase seedling standing rate. This study was performed to research of plug-seedling cultivation techniques of Panax ginseng C.A. Meyer for increase of seedling standing rate. Methods and Results : Testing material, Panax ginseng C.A. Meyer seed, was sown on 5 kin ds of seedling trays (72, 105, 128, 162, 200 cells) in the middle of February 2016. It was gr own in the seedling green house in medicinal plant resources research department of Gyeongs angnam-do Agricultural Research and Extension Services. Four months after sowing, the seedli ng growth characteristics such as seedling standing rate and seedling withering rate were inves tigated. After transplanting in the mountain area of Hamyang-gun, growth characteristics of the root length, root weight, and other factors were investigated at the beginning of November. A s a result, seedling standing rates were 50.1% to 56.2% and seedling withering rates were 25. 2% to 35.9%. Among the five ports, the seedling standing rate of 105 cells and 72 cells was 56.2% and 55.7%, which were higher than others. In the case of 105 cells, however, seedling withering rate was 35.1% higher than that of 72 cells. Seedling leaf length and width were lo nger at 72, 105, 128 cells than at 162, 200 cells and seedling root length, root fresh weight and shoot fresh weight were the highest at 72 cells. Roughly, the more tray cells, the less se edling growth. After harvesting, among the five ports, root fresh weight and root dry weight per 1 hill of 128 cells were 0.36 g and 0.11 g, respectively, which were higher than those of other cells. Conclusion : The growth characteristics of Panax ginseng C.A. Meyer considering the seedling standing rate and seedling withering rate, among the five ports, 72 cells was most suitable. A mong the five ports, considering root fresh weight and root dry weight after harvesting, 128 c ells was most suitable.

Background: The production method of ginseng seedlings for ginseng cultivation is very important to ensure healthy rooting system as well as high quality, and yield of the resultant plants. This study was carried out to compare the growth characteristics of 2- year-old ginseng plants that were produced from seedlings grown in self soil nursery (SSN), nursery soil (NS) or hydroponic culture (HC). Methods and Results: The shading prop used was composed of four-layered 4 polyethylene (blue 3 + black 1) shade screen. The management of main field was done by inserting oil cake (1,200 ㎏/10 a) and then allowing Sudan grass to grow for a year. Seedling transplantation was carried out on April 6. Root growth was measured on October 25. Root weight was observed to be excellent at 6.0 g, following SSN transplantation. Root length was 21.2 ㎝ for HC seedlings, but these plants had a physiological disorder (i.e., rusty root), in 83.5% plants of this treatment. The ratio of PD/PT (protopanaxadiol saponins / protopanaxatriol saponins) was higher in NS seedlings. Plant analysis revealed that Fe content was lower in HC seedlings with high rustiness. The growth of 2-years-old ginseng was different following these varying seedling cultivation methods, but seedlings from NS were not different from those grown in SSN. Conclusions: For the propagation of 2-year-old ginseng plants, NS seedlings may be a good substitute for SSN seedlings.

Background : The Production of high quality ginseng seedling become increasingly important in the cultivation of ginseng because the quality of ginseng seedling is closely related to yield of ginseng. However, it has become difficult to produce high quality ginseng seedling due to environmental instability such as an increase in the occurrence of abnormal climate and pests, and the difficulty in obtain new place to cultivate ginseng seedling. This study was carried out to analyze the growth characteristics of ginseng seedling according to application of fermented compost during ginseng seedling cultivation Methods and Results : In order to evaluate the effect of fermented compost on the growth of ginseng seedling, fermented compost containing antagonistic microorganism was prepared and applied 300kg per 10a to planned place for ginseng cultivation. After seeding of the ginseng seeds, the growth of ginseng was analysed. The pH, EC, organic matter, P2O5 and NO3-N of the compost treated group were 6.1, 2.75 ds/m, 18.97 g/㎏, 1,202 ㎎/㎏ and 287.4 ㎎/㎏, respectively, while those of non-treated group were 6.6, 0.25 ds/m, 14.67 g/㎏, 1,055 ㎎/㎏ and 8.77 ㎎/㎏, respectively. As a result of analyzing the growth characteristics of ginseng seedling, the growth of aboveground part was not significantly different between the treated and non-treated group. The root length and root diameter were 13.0 ± 1.50 ㎝ and 5.5 ± 0.27 ㎜, respectively, which were not significantly different from non-treated group. However, root weight was 1.20 ± 0.14 g, which is 1.4 times higher than that of control. Conclusion : It was confirmed that the quality of ginseng seedling was improved by compost application. These results suggested that fermented compost could be used as a material for nutrients management in ginseng seedling cultivation.

Background : The production of healthy ginseng seedling is a critical part of growing 5-6-year-old fresh ginseng. Recently, for the problem of replant failure, it becomes more and more difficult to find first planted field for ginseng seedling cultivation. In this situation, This study was conducted to examine the possibility of seedling production practices of ginseng by utilizing the multi-beds cultivation system as a way to produce ginseng seedling stably. Methods and Results : This experiment was conducted by installing a 3-layed cultivation beds using 50㎜ sandwich panels. As the 1st and 2nd beds get insufficient amount of light, fluorescent lights were installed in 2 rows to further lighten up. And the 3rd bed used natural light. The bed soil used was formulated by mixing saprolite, yakto and peat moss. The control was cultivated under natural light on 1-layed bed with commercial bed soil for ginseng seedling. The commercial bed soil had pH 7.24, higher than the standard of permission, 6.5; and its calcium and sodium contents were 10.7, and 0.85 cmol+/㎏, respectively, higher than the standard. The available phosphate concentration was 34.0㎎/㎏, lower than the appropriate level of 100 ㎎/㎏. The mixing bed soil also had pH 7.69, higher than the standard but its nitrate and phosphate were within the appropriate range. The intra-facility light quantities of the 1st and 2nd beds were constant at around 100 μmol during days. But the light quantity of the 3rd bed was 51.4 μmol in average daily value. The roots of the 1st-3rd beds were found to weigh 0.58 – 0.73 g while those of the control group, about 0.47g to show heavier root weight of the multi-beds cultivation ginseng than that of the control. As for the yield, the control showed 145 ㎏/10a whereas the 1st-3rd beds showed higher levels between 183 – 228 ㎏/10a. Conclusion : In the multi-beds cultivation of ginseng seedling, fluorescent lamps seem to have provided sufficient light as an artificial light source. The soil for ginseng seedling production is different from ginseng cultivation field soil, additional study will be also necessary to set up the physiochemical range of bed soil for ginseng seedling.

Background : This study was carried out to understand the effect of seedling weight (SW) on growth and flowering in Panax ginseng. Methods and Results : The testing materials were Chunpoong (CP), Yunpoong (YP) and Jakyeongjong (JK). The increase of seedling (1yr) weight led to an increase in ratio of flowering plant and in number of flower per plant. The seed setting rate of two year-old plant (CP, YP, JK) increased with increase of SW at the planting time (PT) and number of flower per plant of three year-old plant (CP, YP) increased also. In the two year-old plant (JK), the ratio of three leaves per plant was 8.8, 19.6, 31.0, 42.0, 44.7 and 58.2%, respectively, in the SW of >0.6, 0.6~0.8, 0.8~1.0, 1.02~1.2, 1.2~1.4 and 1.4g<. The growth of ginseng plant was good with increase of SW at the PT. Conclusion : There was a highly positive correlation between seedling weight and flowering characteristics.

Background : The research is designed to investigate the optimal cultivation technology and the growth of above-ground and below-ground sections as well as the photosynthetic characteristics for new ginseng variety “K-1” by differentiating the planting density under the conditions of transplanting and direct seedling. Methods and Results : The K-1 variety and hybrid variety (Jakyungjong) were selected for the research and the ginseng varieties were transplanted and directly sown in Yeoncheon area in 2013. The transplanting was made in the form of 5 lines × 9 rows (45 plants), 6 lines x 9 rows (54 plants), 7 lines × 9 rows (63 plants) and 8 lines × 9 rows (72 plants) in each lot (1.65㎡) while the direct seedling for testing was conducted three times in randomly blocked design in the form of 11 lines × 14 rows (154 plants), 12 lines × 14 rows (168 plants), 13 lines × 14 rows (182 plants). Various measures were collected from the 4-year transplanted ginseng and 3-year direct seedling ginseng in 2015 to find out the growth features and photosynthesis of above-ground section (rate of germination, leaf length, leaf width, stem length and leaf area index (LAI)) and the below-ground section (length, diameter, weight and class of roots). Conclusion : After the planting of the ginseng, the germination rate of K-1 for the transplanting was 85.1 ~ 92.0% across different plantation densities while that for the direct seedling was 67.7% ~ 77.9% across plantation densities, thus showing no significant difference between the two planting methods. LAI was higher for the higher planation density for both transplanting and direct seedling. As for the photosynthesis speed, the form of 6 lines × 9 rows showed the higher speed in transplanting while the form of 12 lines x 14 rows showed the higher speed in direct seedling. The photosynthesis of K-1 was higher than that of Jakyungjong. In the 4-year ginseng cultivated under the transplanting, diameter of roots, number of branch roots and weight of raw ginseng were the highest in the plantation density of 5 lines × 9 rows. The distribution of root weight was high with 23.3% and 20.0% for the 51~70g group and the 71g or above group, respectively, for the 4 year transplanted plants in the form of 5 lines × 9 rows. The growth for above-ground and below-ground sections for K-1 was better than that for Jakyungjong. As a result, it was found that the proper plantation density for the 4-year root in the transplanted K-1 was 5 lines × 9 rows considering the growth of the above-ground section, quantity and distribution of root weight.

Background : Most ginseng seedlings are cultivated in conventional shading facility. However, due to recent climate changes and soil-related bacterial issues, the production of healthy ginseng seedling with its rhizome weigh more than 0.8g has greatly dwindled. Hence, growing seedling system that raises ginseng on nursery soil in greenhouse is being more highlighted. Nevertheless, there are no sufficient researches conducted to study the growth stability after transplantation of ginseng seedling grown at nursery (GSGN). Methods and Results : After categorizing GSGN produced in 2014 according to individual weight (0.5-0.6g, 0.6-0.7g, 0.7-0.8g, 0.8-0.9g), those GSGN with 0.8-0.9g conventional ginseng seedling (CGS) have been transplanted on preparation field in 17, March of the following year. It has turned out that there were no significant differences about seedling emergence rate and weights between GSGN and CGS both same seedling weight of 0.8-0.9g in 25, June after 100 days transplantation. The 2-year-old ginseng growth of transplanted GSGN according to weight has shown strongly positive correlation between the weight of ginseng seedling and their growth. Especially the leaf area and weight have shown significant correlations represented as Y=212.15-567.32X+499.5X2(R2=0.9988) and Y=4.9-12.9X+11.17X2(R2=0.9788), respectively. Meanwhile, there was no significant correlation noticed treatment plot in relationship between seedling emergence rate and chlorophyll content. Conclusion : With the same root weight of GSGN and CGS, it can be judged that the growth of the 2-year-old GSGN is stable.

Background : Considerable time and effort is required to develop new Panax ginseng varieties. Ginseng breeders have been developing techniques to shorten the breeding cycle to resolve this problem. In this study, we investigated the effects of adding GA3 and alternating temperature (AT, 2℃→ −2℃ → 2℃) on breaking bud dormancy in the varieties (Chungsun and Sunun) of ginseng root. Methods and Results : The GA3 soaking treatment and AT were applied to one year old roots, which greatly accelerated the emergence of new buds. In one year old roots, new buds emerged from the 4th day post transplanting and after breaking dormancy with GA3 and AT treatments. The emergence of new buds was completed within two weeks. The rate of bud emergence for Chungsun was 60% - 98% over 15 - 60 days after the AT and GA3 treatments. The emergence rate of Sunun was 46% - 92%. Normal growth of the ginseng seedling was observed in spite of the early breaking of bud dormancy by combined GA3 and AT treatments. Conclusions : GA3 and AT treatments shortened the dormancy period and facilitated the stable emergence of ginseng seedlings. However, some plants suffered deformities and early sprouting owing to the combined GA3 and AT treatments. Early sprouting was free from dormancy after leaf fall from the of aerial part of the plant.

This study was performed to investigate the effects of sowing density and number of seeds sown on the emergence rate and growth characters of Panax ginseng C. A. Meyer under direct sowing cultivation in a blue plastic greenhouse. Ginseng seedlings, derived from seeds sown directly at different densities (90, 108, 135, and 162 seeds per 162m2), were cultivated in sandy loam soil within a blue plastic greenhouse. In contrast to the emergence rate, which decreased with an increase of sowing density, number of survival plant showed an increasing trend. Interestingly, the emergence and number of survival plant were significantly enhanced when 2 or 3 seeds were sown per hole compared with when one seed was sown per hole. Growth of the aerial parts of ginseng were not markedly influenced by sowing density or the number of seeds sown. However, chlorophyll content (SPAD values) increased with an increase in sowing density. Root parameters, such as root length, diameter, and weight, and the number of lateral roots decreased with an increase in sowing density, but were not noticeably influenced by the number of seeds sown. Total saponin content was the highest in the treatment plot containing 135 seeds. Similarly, the content of each ginsenoside was also tended to be higher in this treatment than in other treatment plots. On the basis of the results obtained in this study, it was possible to determine the optimal sowing density and seed number for the direct sowing cultivation of ginseng in blue plastic greenhouse.

To control the disease of root rot in ginseng nursery, inorganic sulfur solution of 0.1%, 1.0%, and 2.0% were irrigated by amount of 10ℓ per 3.3m2 before sowing. On the last ten days of July, Fusarium solani and F. oxysporum were similarly detected by 44.8% and 43.8%, respectively, while Cylindrocarpon destructans was low detected by 4.4% in the diseased seedling. The more sulfur's concentration was increased, the more soil pH was decreased. Soil pH was decreased from 5.87 to 4.59 by the irrigation of sulfur solution of 1.0%. The more sulfur's concentration was increased, the more electrical conductivity (EC) of soil was increased. EC was increased from 0.27 dS/m to 1.28 dS/m by the irrigation of sulfur solution of 1.0%. Irrigation of sulfur solution was effective on the inhibition of damping-off caused by Rhizoctonia solani in ginseng seedling. Control value for damping-off by the irrigation of sulfur solution of 1.0% and 2.0% were 75.7%, and 78.5%, respectively. Growth of leaf was inhibited by the irrigation of sulfur solution of 2.0%. Root weight per 3.3m2 showed the peak in sulfur solution of 1.0%, while survived-root ratio and root weight per plant were decreased in the level of 2.0%. Survived-root ratio of seedling in sulfur solution of 1.0% was distinctly increased by 4.7 times compare to the control, but control value for root rot was relatively low as 49.2%. Mycelium growth of C. destructans, F. solani, and R. solani were distinctly inhibited by the increase of sulfur's concentration in vitro culture using PDA medium.