밀은 세계 3대 주요 작물이지만 우리나라는 대부분 수입에 의존하고 있으며, 자급률이 1%도 도달하지 못한 실정이다. 국내 밀 품종은 40여 종 이상으로 지속적으로 개발되고 있으며, 품종마다 용도와 농업적 특성이 달라 목표 형질에 맞는 적절한 품종을 선택하여 재배하는 것이 중요하다. 품종을 정확하고 빠르게 판별하기 위해선 분자 마커 기술이 필요하다. 분자 마커는 생육 환경과 시기에 영향을 받지 않고 다양한 품종을 신속하고 정확하게 구분할 수 있어 유전적 변이성, 농업 형질 등을 분석하기에 유용하다. 본 연구는 기존에 보고된 국내 밀 32품종에 대한 SCAR (Sequence Characterized Amplified Region) 판별 마커를 재검정하여 재현성이 높은 마커를 선별하였으며, 기존에 검정되지 않은 국내 밀 9품종에 추가 적용하여 비교분석하였다. 15개의 마커 세트 중 6개의 마커가 재현성과 정확도가 높은 것으로 확인되었고, 최종적으로 국내 41품종 중 4, 7, 8, 10, 11, 12번 마커를 이용하여 다홍, 금강, 밀성, 조은, 수강, 한백, 조광, 영광을 판별할 수 있었다. 또한, 4번 마커를 통한 증폭산물의 염기서열 분석을 통해 SNP (Single Nucleotide Polymorphism)를 발굴하여 ‘올밀’에 대한 새로운 품종 판별 마커인 SdHRM1과 SdHRM2를 개발하여 HRM (High Resolution Melts) 분석을 시행하여 육안으로 판단하기 어려운 SNP를 정확하게 판별할 수 있었다. 품종간의 SNP를 활용한 품종 마커 기술은 다양한 농업형질에 적용할 수 있으며, 분자 육종 프로그램에 실질적인 도움이 되는데 큰 기여를 할 것으로 사료된다.

본 연구는 제6기(2013년~2015년) 국민건강영양조사 결과 중에서 중년남성 882명을 대상으로 밀가루음식 주당 섭취빈도에 따른 건강관련요인, 영양소 섭취, 식행동, 건강지표의 차이를 비교하고자 하였다. 그 결과를 요약하면 다음과 같다. 1. 밀가루음식 저섭취군(54.0%)의 비율은 고섭취군(46.0%) 보다 높았다. 그리고 전체의 밀가루음식 주당 섭취빈도는 4.80회이었으며, 저섭취군(1.78회)과 고섭취군(7.83회)의 섭취빈도 간에 큰 차이를 보였다(p<0.001). 밀가루음식 종류별 섭취빈도를 보면 면류, 과자류, 빵류 순이었다. 2. 건강관련 요인에서 살펴본 월 1회 이상 음주 여부와 현재 흡연 여부, 스트레스 인지율, 1일 평균 수면시간, 1주일간 근력운동과 걷기 일수에서 밀가루 섭취빈도에 따른 유의적인 관련성과 차이는 없었다. 3. 1일 에너지 평균섭취량은 1,970.45 kcal이었고, 저섭취군 보다 고섭취군에서 약 630 kcal 정도 더 높았다(p<0.001). 그리고 고섭취군에서 상대적으로 당질 섭취비는 낮은 반면, 지방과 단백질 섭취비는 더 높았다(p<0.001). 무기질과 비타민의 1,000 kcal당 영양소 밀도에서 칼슘, 인, 나이아신을 제외하고, 모두 유의적인 차이가 있었다. 칼륨, 철분, 비타민 A, 비타민 B1, 비타민 C에서는 저섭취군의 섭취가 더 많았던 반면, 나트륨, 비타민 B2에서는 고섭취군의 섭취가 더 많았다 (p<0.01, p< 0.001). 4. 식행동에서 1일 식사횟수는 밀가루음식 저섭취군에서 고섭취군에 비해 3회 비율이 더 높아 관련성을 보였다(p<0.05). 외식빈도는 저섭취군(46.9%)보다 고섭취군(43.2%)에서 더 낮은 경향을 보였으나, 유의성은 없었다. 구입 시 영양표시 이용 여부는 고섭취군(20.5%)이 저섭취군(15.7%)에 비해 더 이용하고 있었으나, 유의적인 관련성은 아니었다. 5. 건강지표에서 신장과 체중, 허리둘레 값이 고섭취군에서 더 높은 경향이 있었으나, 신장(p<0.01)에서만 유의적인 차이가 있었다. 그리고 총콜레스테롤과 LDL-콜레스테롤에서 고섭취군이 저섭취군보다 더 높은 값을 나타내었다(p<0.01). 혈청 중성지방에서는 저섭취군이 고섭취군보다 10.5 mg/dL 정도 더 높은 값을 보였으나, 유의적인 차이는 아니었다. 본 연구결과는 국민건강영양조사 자료를 활용한 횡단면적 연구이어서 인과관계를 밝히기 어렵고, 밀가루음식 섭취량이 아닌 섭취빈도만을 가지고 연구를 행했다는 제한점이 있다. 그러나 대상자 선정에서 교란인자를 제외하였고, 통계분석에서도 보정함으로써 밀가루음식 섭취빈도와 영양소 섭취, 건강지표와의 차이를 독립적으로 측정하고자 하였다. 현재 우리나라에서는 밀가루음식의 섭취가 계속 증가되고 있어 이에 대한 우려가 있다. 실제로 본 연구에서 밀가루음식을 자주 먹는 경우 에너지 영양소의 섭취는 더 많았던 반면 나트륨과 비타민 B2를 제외한 대부분의 무기질과 비타민의 섭취는 더 적은 것으로 나타났다. 그리고 건강지표에서 총 콜레스테롤과 LDL-콜레스테롤 농도가 더 높게 나타나 우려가 현실화 되고 있음을 알 수 있었는데, 본 연구결과가 밀가루음식의 과잉 섭취 시 문제점을 알리는데 활용되었으면 한다.

DNA-based markers have been used in various fields like molecular biology and crop breeding program. DNA marker is able to be used for protection of genetic resource and quantification of specific cultivar. Eleven DNA markers have been developed for Korean wheat cultivar identification in 2013-2014. 27 of 32 wheat cultivars were distinguished by 11 DNA markers. In this study, we developed four DNA markers, KWSM0012, KWSM0013, KWSM0014 and KWSM0015, derived from SSR and SNP analysis. Consequently, 32 Korean wheat cultivars were identified by 15 DNA markers. We are convinced that these new DNA markers are very useful for wheat varieties DNA fingerprinting and are able to be applied to marker-assisted selection in wheat breeding program in Korea.

본 연구에서는 국산밀 품종에 대해 발아시간에 따른 항산 화 성분 및 항산화 활성을 측정하였다. 발아시간에 따른 국산 밀 에탄올 추출물의 총 폴리페놀, 플라보노이드 및 탄닌 등 페놀성분의 함량은 발아시간에 따라 유의적인 차이를 보이 는 것으로 나타났다. 무처리 금강밀, 조경밀, 백중밀 및 고소 밀의 총 폴리페놀 함량은 13.74, 15.05, 16.84 및 13.02 mg GAE/100 g이었으며, 발아시간이 증가함에 따라 유의적으로 증가하는 경향을 보였다(p<0.05). 총 플라보노이드 함량은 각 각 5.11, 6.72, 6.28 및 5.43 mg CE/100 g이었으며, 발아시간이 증가함에 따라 큰 차이를 보이지 않았으나, 유의성을 보이는 것으로 나타났다(p<0.05). 총 탄닌 함량은 각각 9.19, 8.86, 8.93 및 8.66 mg TAE/100 g이었으며, 발아시간이 증가함에 따 라 큰 차이를 보이지 않았으나, 유의성을 보이는 것으로 나타 났다(p<0.05). 금강밀, 조경밀, 백중밀 및 고소밀의 DPPH 라 디칼 소거활성은 각각 30.77, 23.88, 25.35 및 18.73 mg TE/100 g이었으며, 25℃에서 72시간 발아 시료가 각각 47.47, 44.17, 38.22 및 42.85 mg TE/100 g으로 유의적으로 높은 활성을 보 이는 것으로 나타났다(p<0.05). ABTS 라디칼 소거활성은 각 각 83.42, 88.53, 88.87 및 79.97 mg TE/100 g이었으며, 발아시 간이 증가함에 따라 유의적으로 증가하는 경향을 보였다 (p<0.05). 이상의 결과 국산밀의 발아를 통하여 페놀성분 및 라디칼 소거활성이 증진된 효과를 얻을 수 있는 것을 확인할 수 있었다.

The quality characteristics of noodles made from domestic Korean wheat flour added with 0, 1, 3, 5% cactus Chounnyuncho (Opuntia humifusa) powder were investigated. The water absorption and peak time of dough, as determined by farinography, increased with higher cactus powder content. Stability and elasticity of dough decreased as fermentation proceeded. Cooking characteristics of noodles made from dough such as weight, water absorption, and volume decreased with higher cactus powder content. The turbidity of cooked water increased significantly with 5% cactus powder content. For color test, L-value decreased while a-value and b-value increased with higher cactus powder content. In texture analyses, hardness, adhesiveness, springiness, cohesiveness, and gumminess increased with higher cactus powder content. In the sensory test, noodles containing 3% cactus powder were the most preferred.

국내 토종 자원으로 알려진 앉은뱅이 밀의 밀가루 특성 밀 과자 적성을 평가하기 위해서 경남 진주시에서 구입한 앉은뱅이 밀의 통밀가루 및 백밀가루와 중형제분기로 제분한 밀가루를 평가하였다. 앉은뱅이 밀 구입처에서 제분된 제분된 통밀가루와 백밀가루는 중형제분기에서 제분된 밀가루에 비하여 밀기울 혼입으로 인하여, 회분과 단백질 함량이 높고 밀가루 색깔이 어두웠고 침전가와 건부율 및 아밀로스 함량은 낮았다. 반죽 시간이나 안정도는 밀가루간에는 차이가 없었지만, 반죽 가수량은 밀기울을 포함한 통밀가루가 높았고, 쿠키 직경이 적게 나타났다. 중형제분기로 제분된 앉은뱅이 밀가루의 밀가루 입도와 손상 전분 함량이 각각 54.44μm와 2.46%로 연질밀 특성을 나타내었으며, 총아라비노자일란 함량과 수용성 아라비노자일란 함량이 각각 28.52%와 9.49% 로 국내 밀 품종보다 높았다. 쿠키 직경은 86.88mm로 수입산 박력분과 유사하였으며, 쿠키 표면의 균열도 쿠키 적성에 적합한 것으로 나타났다.

분말을 우리밀 밀가루 중량 100%를 기준하여 각각 0.5, 1.0 및 2.0% 첨가하여 제조한 국수의 품질특성을 조사하였다. DPPH radical 소거활성, 환원력, 자동산화 억제활성, tyrosinase 저해활성을 측정한 결과, 분획물의 농도가 증가함에 따라 항산화활성은 증가하는 경향을 보였으며, 부탄올 분획물에서 높은 DPPH radical 소거활성, 환원력, 자동산화 억제활성, tyrosinase 저해활성을 보였다. 솔잎분말을 첨가하여 제조한 국수의 중량, 부피, 함수율 및 국물의 탁도는 대조구와 비교하여 분말의 첨가량이 많을수록 증가하였다. 색도는 솔잎분말의 첨가량이 증가함에 따라 L값과 a값은 감소하였으며 b값은 증가하였다. 솔잎분말을 첨가하여 조리한 국수의 조직감은 우리밀 밀가루만 사용하여 제조한 대조구에 비하여 경도, 응집성, 탄력성, 검성 및 씹힘성이 감소하는 경향을 보였다. 관능검사 결과 솔잎 분말 1.0% 첨가구에서 색, 맛 및 전체적인 기호도가 높게 나타났으며, 향과 조직감에서는 대조구와 비교하여 유의적인 차이를 나타내지 않았다.

This study was performed to assess the level of ozone risk for wheat and potato in the central region of the Korean Peninsula by using ozone indices, the ozone-concentration based index (AOT40). The AOT40 indices were calculated for wheat and potato, respectively, at 25 sites in the central region of the Korean Peninsula during a period of three months from April 1 to June 30, 2006. The results for both indices, AOT40, showed that agricultural crops could be seriously damaged by ozone in the central region of the Korean Peninsula. In the result of AOT40, ozone may affect about 60% of the sites for wheat and 20% of the 25 sites for potato.

Wheat is an economically important cereal worldwide and is a staple food in many countries. Extensive researches have been performed on the nutritional value and antioxidant potentials of sprouts of legumes like soybean, however very limited studies have been carried out on the sprouts of Korean wheat cultivar. The objective of this study was to investigate the phytochemical and antioxidant potentials of Korean wheat sprouts. The sprouts were grown for 10 (WS-10), 20 (WS-20), and 30 (WS-30) days at room temperature. The pH, color, chlorophyll, free amino acid, 1,1-diphenly- 2-picrylhydrazyl (DPPH), and total polyphenol content of the sprout samples were determined. The pH of sprouts was increased in the older sprouts. Color values were significantly influenced by the age of sprouts. The chlorophyll content was lowest but the total free amino acid content was highest in WS-30. On the other hand, the DPPH free radical scavenging potential and total polyphenol content were lower in WS-30. The results suggested that Korean wheat sprouts could be a potential source of nutrients and natural antioxidants.

Yellow alkaline noodles were prepared with various Korean wheat cultivars including different protein and amylose contents and made from two types of noodle machines. Korean food companies and restaurants generally used noodle machine made in Korea, whereas research institutes mainly practiced with noodle machine made in Japan. Noodle dough sheet from Korean machine showed 5% higher water absorption and 30 min shorter resting period than the noodle dough from Japanese machine because of the difference of size and weight of roller in two types of the machines. In 15 Korean wheat cultivars, thickness of noodle dough sheet was positively correlated with protein content and mixograph water absorption regardless of both types of the machines. Cooked noodles from Korean machine showed higher hardness and lower springiness than noodles from Japanese noodle machine, but difference in cohesiveness of cooked noodles was not found between two different noodle machines. In the three Korean wheat cultivars with different protein content, thickness of noodle dough sheet was also positively correlated with protein content, SDS sedimentation volume and mixing time of mixograph in both types of the machines. The more protein content increased, the more hardness of cooked noodles increased regardless of both types of the machines. However, springiness and cohesiveness of cooked noodles were not significantly correlated with protein content. In waxy and partial waxy wheat cultivars, lightness of noodle dough sheet from Korean noodle machine positively correlated with amylose content. Amylose content and setback in pasting properties were positively correlated with hardness, springiness, and cohesiveness of cooked noodles while both damaged starch and breakdown were negatively correlated with those.

Eleven RAPD primers were assessed to analyze genetic diversity of Korean wheat varieties and to develop DNA marker for cultivar identification. The average of the number of polymorphic bands was 5.2 and PIC values showed 0.48, respectively. Ten major clades were presented by phylogenetic analysis. Three cultivars containing Uri, Hanbeak and Jonong were distinct from the others in the phylogenetic dendrogram. Seven cultivar-specific fragments were detected from 11 RAPD fingerprinting among 35 wheat cultivars and they were sequenced. Four Korean wheat cultivars, Eunpa, Jopoom, Yeonbaek and Jeokjoong, were identified newly by four markers, 84, 173, 174 and KWSM011. We convince that these new DNA markers are useful for cultivar fingerprinting and are applied to marker-assisted selection in wheat breeding program.

Mixolab is suitable to evaluate mixograph and farinograph for testing dough rheology and amylopgraph for properties of starch pasting with a view to analyze protein and starch quality at the same time. Mixolab analysis revealed that water absorption and dough development time of Korean wheat cultivars were similar to it of soft wheats and flour while dough stability and protein weakening of Korean wheat cultivars were similar to it of hard wheat and strong flour. Also, starch properties of Korean wheat cultivars showed similar properties of hard wheats. There was a significantly positive correlation between mixolab and mixograph in the water absorption of dough, whereas no correlation was observed between them in dough development time and stability. Furthermore, maximum viscosity of starch and breakdown in amylopgraph were correlated to stabilities of pasting and cooking and setback in mixolab. Resultantly, in mixolab, properties of dough and protein highly and positively correlated to loaf volume, hardness of noodles, and size of cookies i.e. diameter. Moreover, positive correlation was identified between viscoelasticity of noodles and properties of protein, pasting, and setback in mixolab.

This study was executed to establish a basis of evaluation of starch properties of Korean wheat cultivars e.g. damaged starch, swelling, and pasting properties for Korean wheat breeding program. Damaged starch is critical evaluation factor for flour milling related industry because it influences water absorption and color of dough for processing quality and preference of end-use products. The present results revealed that there was significantly high positive correlation between the results of damaged starch analysis by amperometric (SD-matic) and enzymatic (Megazyme assay) methods. Evaluation of damaged starch must be considered as one factor to evaluate properties of starch due to its accuracy and a stable efficiency for the wheat breeding program. Properties of swelling and pasting of dough were important for cooking time and texture. Nevertheless, it was impossible to evaluate starch extracted from flour in the wheat breeding program i.e. small amount of flour or small number of spikes Comparison of results of evaluation of properties of swelling and pasting with starch or flour, the evaluation using flour positively correlated with the other evaluation using starch. In addition, swelling evaluation must be considered to apply for the wheat breeding program because the result of evaluation of swelling property, which possible to evaluate with low efficiency, and the quantity of a sample is highly under positive correlation with paste peak viscosity. In the future, studies using NIR (Near Infrared) analysis must be necessary to evaluate starch properties with grains in early generation lines for improvement of wheat breeding program.

Near infrared reflectance spectroscopy (NIRS) has been used as a rapid analysis tool to many components in cereal grains. This study was to investigate the potential NIRS application for determination of components in Korean wheat. Main components of wheat quality are protein content, moisture content, SDS-sedimentation volume and ash content. Wheat has screened for quality, hardness of seed by NIRS in CIMMYT (International Maize and Wheat Improvement Center). NIRS calibration was used as a rapid and simultaneous analysis method to determine the wheat quality components. A total of 282 wheat samples, collected from a wide range of Korean wheat cultivation region for 2 years, were analyzed by NIRS. NIRS calibration of individual components were developed using first derivation, second derivation and modified partial least-squares regression and internal cross validation method. As a result, calibration formula of protein was y=0.937x+0.786, calibration formula of moisture was y=0.922x+0.911, calibration formula of ash was y=0.933x+0.08, calibration formula of SDS-sedimentation volume was y=0.947x+2.150. NIRS calibration for wheat quality may be useful for determining protein(R2 = 93.6), moisture(R2 = 91.6), SDS-sedimentation volume(R2 = 94.3), and ash(R2 = 93.4). This study shows that 4 calibrations of NIRS is a useful application in the accurate and rapid determination of wheat quality. Therefore, NIRS could be used to rapidly determine the quality contents of wheat for grade evaluation in a purchasing of wheat cultivation region.

Genetic variations of γ-/ω-gliadin and Spa (storage protein activator) in 40 Korean wheat cultivars were evaluated to provide genetic information for improving end-use quality in wheat breeding programs. Korean wheat cultivars were classified into 13 patterns at the Gli-1 locus based on the allelic variation using A-PAGE (acidic-polyacrylamide gel electrophoresis). Seven, five, and six alleles were identified at Gli-A1, Gli-B1, and Gli-D1 loci, respectively. Allele-specific PCR markers for γ-gliadin corresponded to specific allele at Gli-1 loci on A-PAGE, which Gli-A1f, Gli-A1h and Gli-A1l alleles corresponded to GliA1.2, Gli-B1h and Gli-B1f alleles corresponded to GliB1.2 and Gli-D1f, Gli-D1m and Gli-D1o alleles corresponded to GliD1.1. DNA markers for γ-45 and γ-42 also corresponded to the γ-gliadin patterns around 40kDa on A-PAGE, except in Sukang, Ol and Joongmo2003. However, allelic specific PCR markers for ω5-gliadin did not correspond to that of A-PAGE. Three alleles were identified at Spa-A1 locus, whereas there was no variation at Spa-B1 and Spa-D1 loci.

The ω5-gliadins are the major allergens in wheat-dependent excise-induced anaphylaxis (WDEIA). In this study, SDS-PAGE analysis was used to assign the ω5-gliadins (Gli-B1) alleles in thirty two Korean wheat cultivars, compared with eleven standard wheat cultivars for Gli-B1a~m alleles. These results were reconfirmed with their complementary Glu-B3 low-molecular-weight glutenin subunits alleles tightly linked with Gli-B1 locus revealed with 2-DGE in our previous study. As a result, one Gli-B1b, four Gli-B1d, two Gli-B1f, six Gli-B1m and nineteen Gli-B1h varieties were identified. This is the first report on revealing the Gli-B1 alleles in Korean wheat cultivars and represents valuable basic data on wheat allergy, relationship between gliadin and wheat quality, and development of hypo-allergenic wheat.

High temperature is one of major environmental stress. Heat tolerance managing is difficult through the phenotypic selection, so marker assistant selection (MAS) using molecular markers like as RAPD, SSR etc. was tried to select useful traits for heat tolerance. Fourteen SSR markers reported by previous research were selected for this research. We tried to evaluate 14 SSR markers for MAS using 31 useful wheat resources including 24 crossing line from Turkey, six Korean wheat cultivars and Chinese spring. The average of the number of alleles and PIC values in this study were 6.14 and 0.64, respectively. Two major clades and four sub clades were grouped by phylogenetic tree using UPGMA. Four Korean wheat cultivars were distinct from other Turkey resources in the phylogenetic dendrogram. From the results, we expected that these markers were able to adapt to screening wheat genotyping for heat tolerance.