본 연구에서는 백삼 및 홍삼 추출물과 그 효소 가수 분해물을 제조하고 이를 첨가하여 제조된 양갱의 항산화 활성 및 품질 특성을 조사하였다. 백삼과 홍삼 추출물의 효소 가수분해를 위해서는 Rapidase C80 max, Pyr-flo, Ultimase MFC를 선정하였다. Rapidase C80 max, Pyr-flo, Ultimase MFC로 가수분해 한 백삼과 홍삼에서는 효소반응 전에는 검출되지 않았던 ginsenoside F2와 Compound K (CK)가 검출되었으며 특히 홍삼의 2차 효소 반응군에서 CK의 함량이 가장 높았다. 효소 가수분해 전 또는 후의 백삼 및 홍삼을 함유한 양갱을 제조한 후 폴리페놀 함량, 항산화 능력을 분석한 결과 백삼 및 홍삼을 함유하지 않은 양갱(대조군)에 비해 총 폴리페놀 함량, DPPH 라디칼 소거능, ABTS 라디칼 소거능, FRAP 분석 결과 모두 우수하였으며, 첨가한 백삼 및 홍삼에 효소반응을 진행할수록 유의적으로 항산화 활성이 증가하였다(P<0.05). 밝기 (L*)는 무첨가 양갱(대조군)이, 적색도(a*)는 홍삼양갱(RG) 이, 황색도(b*)는 백삼양갱(WG)이 높게 나타났고, 조직감 은 홍삼 첨가 후 2차 가수분해까지 진행한 양갱(RG-T2) 이 강도(hardness), 탄력성(springiness), 씹힘성(chewiness), 응집성(cohesiveness), 검성(gumminess) 모두 유의하게 높은 결과를 나타내었다(P<0.05). 결론적으로, 백삼 또는 홍삼 추출물에 Rapidase C80 max, Pyr-flo, Ultimase MFC를 처리하면 진세노사이드 탈당화에 매우 유용하여 생리활성이 우수한 CK를 생산할 수 있으며, 효소로 가수분해 된 백삼 및 홍삼을 첨가한 양갱은 대조군(인삼 무첨가 양갱) 에 비해 총 폴리페놀과 항산화 활성을 유의하게 증가시킨다는 것을 알 수 있었다. 이러한 결과는 향후 기능성 양갱 제조에 우수한 기초자료로 활용될 수 있을 것으로 보인다.

This study investigated properties of bread utilizing extracts of ginseng powder. Ginseng powder bread was baked. Addition of 1~3% of ginseng powder extract to wheat flour was carried out. Rheological properties, dough pH, dough volume, bread volume, water absorption capacity, baking loss, bread color, bread texture, and sensory evaluation were tested to examine properties of bread baked with extracts of ginseng powder. The results are as follows. The dough pH decreased gradually with increasing ginseng powder extract concentration. The bread volume, baking loss, and bread weight increased with increasing use of ginseng powder extract, and springiness and cohesiveness increased as red ginseng powder additive concentration increased. Lightness of the L decreased while a and b increased. In the sensory evaluation, ginseng powder with 1% ginseng powder extract was evaluated as the best for taste, texture, flavor, and overall acceptability.

1983-1984 제(第)에 걸쳐 한국인삼연초연구소 수원경작시험장 인삼포(人蔘圃) 및 그 주변에서 궁뱅이를 채집(採集)하여 외부형태상(外部形態上)의 특징, 종구성(種構成) 및 인삼가해여부(人蔘加害如否)를 조사(調査)하여 다음과 같은 결과를 얻었다. 1. 채집(採集)된 굼뱅이는 항문공(肛門孔)의 모양과 여상부에 있는 융기선(隆起線)의 유무(有無)에 따라 검정풍뎅이 아과(亞科), 줄풍뎅이 아과(亞科), 우단풍뎅이 아과(亞科)로 나뉘어지고, 이들은 다시 두부(頭部)나 제10복절(第10腹節) 복면(腹面)의 자모(刺毛) 배열(配列), 두개(頭蓋)의 광택 정도에 띠라 8종(種)으로 분류(分類)되었다. 2. 인삼포(人蔘圃) 주변의 포장(圃場)이나 휴한지(休閑地)에서 주로 다색풍뎅이 애풍뎅이 다색줄풍뎅이 참검정풍뎅이 큰검정풍뎅이 등이 채집(採集)되었으나, 인삼포(人蔘圃)에서는 참검정풍뎅이와 큰검정풍뎅이만이 발견되었으며 이들의 인삼(人蔘) 가해(加害)가 확인되었다.

Background: The ginsenosides Rb1 (G-Rb1) and Rg1 (G-Rg1) are used as marker compounds, and are the principal bioactive compounds assessed in the quality control of white ginseng. This study was conducted to analyze white ginseng samples of different and to obtain useful data for the quality control of white ginseng. Methods and Results: The variation in the content of G-Rb1 and G-Rg1 was evaluated among 35 samples of 4-, 5-, and 6-year-old white ginseng. The content of both G-Rb1 and G-Rg1 did not significantly differ among ages, and the relative ratio of the maximum to the minimum content of these within ginseng of the same ages was more than two. However, the ratio of G-Rb1 to G-Rg1 content in the 5- and 6-year-old ginseng was significantly higher than that in the 4-year-old one. According to the ‘Ginseng industrial act’, the standard (w/w, %) minimum G-Rg1 and G-Rb1 content is 0.10% and 0.20% or more, respectively. Among the 35 samples examined, the content of G-Rg1 was found to be 0.124 - 0.399% with none being less than the standard level, while that of G-Rb1, was 0.147 - 0.595%, with 4 samples (11.4%) failing to meet the standard levels. The content of G-Rg1 and G-Rb1 did not show a constant relationship with the size of ginseng. Conclusions: In our study, the content of both G-Rg1 and G-Rb1 varied widely, and there was no significant difference among cultivation ages. The results of the present study might provide useful information for the quality control of raw ginseng and processed white ginseng using marker compound.

Background: To enhance the taste and physiological characteristics of Lycii fructus (Gugija) extracts, we investigated the changes in the physiological characteristics of Gugija extracts caused by adding white ginseng (WG) and red ginseng (RG) Methods and Results: Gugija extracts, including 10G10, 10GW-G8 : 2, -G6 : 4, -G4 : 6, -G2 : 8, and -G0 (mixtures made by replacing 0, 20, 40, 60, 80, and 100% of Gugija with WG), as well as 10G10, 10GR-G8 : 2, -G6 : 4, -G4 : 6, -G2 : 8, and -G0 (mixture made by replacing 0, 20, 40, 60, 80, and 100% of Gugija with RG) were extracted with water at 10 times the respective mixture's volume. The antioxidant activities of Gugija extracts were investigated by assessing their 1,1-diphenyl-2-picrydrazyl (DPPH) and 2,2’-azinobis(3ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, ferric reducing antioxidant potential (FRAP) activity, nitrite scavenging activity, and angiotensin converting enzyme (ACE) inhibitory activity. As the amount of WG added increased, the DPPH, and, ABTS radical scavenging activity, and FRAP activity of the Gugija extract decreased. The half maximal inhibitory concentration (IC50) value of 10G10, 10GW-G6 : 4, 10GR-G6 : 4, and 10GR-G0 for DPPH radical scavenging activity were 25.50 ± 1.04, 52.06 ± 1.46, 16.87 ± 1.24, and 9.50 ± 0.16 ㎕/㎖, respectively. On the other hand, the physiological activity of Gugija extract increased with the addition of increasing amounts of RG. However, ACE inhibitory activity was the highest (50.25 ± 2.58%) in the Gugija 10-fold extract without any added RG. Conclusions: From the above results, we suggest that adding RG to Gugija extracts increase their antioxidant, FRAP, and nitrite scavenging activities.

Background : This study was conducted to investigate the effect of soluble silicate and germanium on the characteristics of soil and growth in ginseng (Panax ginseng C. A. Meyer) cultivated in blue-white plastic houses. Methods and Results : Seedlings (n = 63 per 3.3 ㎡) of Korean ginseng cultivar were planted on April 10, 2015. Shading material of plastic house [8 m (W) × 3.5 m (H) × 50 m (L)] was blue-white vinyl. From May to September of each year, 1,000 times diluted sol. of germanium and silicate and these mixed solutions were drenched once a month. The chemical properties of the soil are as follows : pH, EC, organic content, and P2O5 of the soil were within the proper range, and the Mg content of the Ex-cations was lower than the standard value in 3 treatments except treatment of germanium 1,000 times. The total nitrogen content was 0.13 - 0.14%, which was higher than the optimum value. The number of soil microorganisms (Actinomycetes etc.) was higher in the treatment than in the non-treatment of inorganic components. The photosynthetic rate of ginseng leaves was 2.25 μmole CO2/㎡/s in the non-treatment and 2.91 to 3.17 μmole CO2/㎡/s in the inorganic treated. The growth characteristics of the aerial parts (leaf and stem) of the ginseng plants were generally better than that of non-treatment. Especially, in the treatment with silicate 1,000 times showed better growth, leaf area and leaf width growth. The growth of the underground part (root weight etc.) were also better than non-treatment. Specially, at 1,000 ppm treatment with soluble silicate, the root length and diameter were longer and the weight was was 72.2 g per plant. The ginsenoside content of each treatment is under analysis. The disease incidence (Alternaria panax etc.) rate was 20.1% in the untreated and 6.4% to 10.0% in the inorganic components treatment. Conclusion : By soil drench of inorganic compononts such as soluble silicate in ginseng blue-white plastic house cultivation, the growth of the aerial and root parts in ginseng were more improved than non-treatment. There are a lot of soil microorganism and less disease.

Background : The fruit of Lycii fructus (Gugija) has beeen used as a tonic medicine and a long-term healthy food without side effect in Asia. An increase in the demand for natural healthy food, Gugija has been thought as a source of healthy foods, and then the extracts of dried Gugija have been frequently used as food ingredients. But, its extracts with Gugija alone has a slightly bad taste. Physiological characteristics of Gugija extracts were investigated by adding white ginseng (WG) and red ginseng (RG) to enhance taste. Methods and Results : Gugija extract were prepared by adding 0, 20, 40, 60, 80 and 100% of ginseng (WG and RG) to the weight of Gugija, respectively. Antioxidant activities of Gugija extracts were investigated DPPH and ABTS radical scavenging activity, SOD-like activity, FRAP, activity. As the amount of WG added increased, DPPH, ABTS radical scavenging activity, and FRAP activity of Gugija extract decreased. On the other hand, the physiological activity of Gugija extract increased with increasing amount of RG. The SOD-like activity of Gugija extract added up to 60% of WG and to 40% of RG was continuously lowered, in the addition amount thereafter, the activity increased as the addition amount increased. In components, As the amount of WG and RG added increased, the ginsenosides content of Gugija extract increased, the content of ginsenosides was higher in extract added RG than WG, but betaine and total flavonoid content decreased. Conclusion : From the above results, we may suggest that Gugija extracts by adding RG increased the antioxidant activities, SOD-like activity, and the preference degree.

Background : Korea ginseng root has been traditionally used as a tonic as it is stated to have the capacity to normalize body functions and strengthen systems that are caused by various stresses. But, white ginseng (WG) has lower antioxidant activity than other medicinal crops. Omija (Schisandra chinensis Baillon) has various physiological functionalities such as anti-cancer, anti-inflammatory, and antioxidant activities, which have the effective components of Omija are lignans (schizandrins and gomisins), and this components were contented mostly in seed part on Omija. Physiological characteristics of WG extracts were investigated by adding Omija to enhance functionality. Methods and Results : WG extract were prepared by adding 0, 20, 40, 60, 80 and 100% of Omija to the weight of WG. Physiological characteristics of WG extracts were investigated DPPH radical scavenging activity, FRAP, and nitrite scavenging activity. As the amount of Omija added increased, DPPH radical scavenging activity of WG extract increased proportionally. IC50 of 10 times water extracts were 0.455, 0.028 and 0.041 ml/ml on WG (100WG), WG added 50% Omija (50OM50WG), and Omija (100OM0WG), respectively. On the other hand, FRAP of extracts decreased slightly with increasing amount of Omija. Nitrite scavenging activity of Omija extract were significantly more active than WG extract, exhibited to 88.89% and 72.65% on WG extract added with 40% Omija (40OM60WG) and non added (100WG), respectively, pH 1.2. and then, to 33.45% and 1.38% with 40OM60WG and 100WG extract, respectively. Conclusion : From the above results, we may suggest that WG extracts by adding Omija increased the DPPH radical scavenging activity, nitrite scavenging activity and the preference degree on taste.

Background : This study was conducted to examine the suitability of the blue/white vinyl for ginseng cultivation greenhouses in different regions in an effort to develop a stable ginseng cultivation technology and contribute to popularizing ginseng greenhouse farming. Methods and Results : For comparison purposes, ginseng plants were cultivated in the traditional shading and greenhouse shading facilities. The average temperature in the greenhouse shading facilities during the period of May through October was 20.5℃ in Namwon, 21.4℃ in Jinan, and 23.3℃ in Iksan, with Iksan recording a 1.9–2.8℃ higher average temperature, presumably due to its location in a plain area with low elevation. The average temperatures in the traditional shading facilities ranged between 22.9℃ and 25.7℃, 2.4–2.6℃ higher compared with the greenhouse shading facilities. As for the incidence of disease and pest, as low as 0.1–0.3% of the plants grown under greenhouse shading were infected with Colletotrichum dematium, Alternaria panax, and Rhizoctonia solani, whereas much higher incidences were noted in the control plants, with 3.3–11.3%, 4.7–17.0%, 0.1–0.5%, and 3.0–5.7% infected with Colletotrichum dematium, Alternaria panax, Rhizoctonia solani, and Phytophthora cactorum, respectively, throughout the regions. Heat injury occurred only in Iksan (0.3%) in the case of greenhouse-shaded plants, whereas the control plants suffered from much severer heat injury: 3.5% in Namwon, 10% in Jinan, and as high as 35.3% in Iksan. As for the roots, Namwon outperformed other regions in root length (29.7 ㎝), taproot length (8.7 ㎝), and taproot diameter (25.3 ㎜), and also the root fresh weight (53.4 g), followed by Jinan (48.7 g) and Iksan (40.4 g). In yield as well, the greenhouse in Namwon outperformed other regions with 1,297㎏/10a, followed by Jinan (1,183 ㎏) and Iksan (932 ㎏). The effect of greenhouse blue-white vinyl shading on yield by region was analyzed to increase of 177% in Namwon, 209% in Jinan, and 173% in Iksan with respect to their respective traditional shading facilities. Conclusion : The suitability of the blue/white vinyl shading in ginseng cultivation greenhouses was tested experimentally in three different regions. As a result, greenhouse-shaded ginseng plants were found to be much less affected by heat injury compared with traditionally shaded ginseng plants, resulting in much higher yields in all regions. This allows the conclusion that greenhouse ginseng cultivation can contribute to more stable ginseng yield and popularization of ginseng cultivation.

Background: The study was conducted to elucidate the extraction conditions under which white ginseng has cognition-improving efficacy.Methods and Results: Extracts from white ginseng under different solvent and temperature conditions were analyzed for ginsenoside content and inhibitory effect on N-methyl-D-aspartate (NMDA) receptor and acetylcholinesterase. The total ginsenoside contents and amounts of ginsenoside Rb1 plus ginsenoside Rg1 from the 1st extracts (prepared with EtOH/H2O as solvent) were higher than those from the 2nd extracts (extracted with H2O after the 1st EtOH/H2O extraction). The contents in the 1st and 2nd extracts produced at 80°C were also higher than those obtained at 50°C. Samples from the 1st extraction at 80°C indicated higher inhibitory activities on NMDA receptors-whose excessive activation is thought to mediate the calcium-dependent neurotoxicity associated with several neurodegenerative diseases-than those from the 2nd extraction. Among the samples prepared at varying temperatures, the extract prepared at 50°C showed the highest suppression activity on NMDA receptors. Note, however, that the extracts from the 2nd extraction at 50°C inhibited acetylcholinesterase-whose inhibition could be a therapeutic strategy for neurodegenerative diseases with cognitive deficits and memory malfunction-more effectively than those from the 1st extraction.Conclusions: To enhance the cognition-improving activity of white ginseng extract, it is suggested that the extracts be utilized after being combined the 1st extracts (made with EtOH/H2O solvent) and the 2nd extracts (prepared with H2O) at low temperature.

Background : Malonyl ginsenoside content of the Panax ginseng is known to account for 35% to 60% of total ginsenosides content. However, its distribution by ginseng part has not been studied. In this study, four kinds of malonyl ginsenosides were compared in Korean white ginseng part using the purified malonyl ginsenoside standards in our laboratory. Methods and Results : White ginseng was prepared by the air drying (50℃, 48h) or freeze drying (-70℃, 48h) methods form 4-year-old ginseng. Malonyl ginsenoside content in total ginsenosides were similar in air dried and freeze dried white ginseng, 58% and 62%, respectively. Therefore, malonyl ginsenoside contents in main, lateral, and fine root, and in the main root without skin and skin of main root prepared by freeze dried method were compared. Malonyl ginsenosides (m-Rb1, m-Rb2, m-Rc and m-Rd) and total ginsenosides (Rg1, Re, Rf, Rb1, Rc, Rb2, Rb3, Rd, m-Rb1, m-Rb2, m-Rc and m-Rd) were 6.75 and 14.15 mg/g in main root, 14.15 and 26.35 mg/g in lateral root, 46.95 and 84.15 mg/g in fine root. Malonyl ginsenoside contents in skin of main root was 20.08 mg/g, while its contents of the main root without skin was 2.58 mg/g. Conclusion : As a result, the parts each air drying the sample was confirmed that the ratio of the distribution of malonyl ginsenoside (main root : lateral : fine root = 18.7 : 11.1 : 16.2), and distribution ratio of main root, skin of main root, lateral, skin of lateral was found to be (12.2: 14.6 14.3: 3.7). Malonyl ginsenoside content was the highest in fine root, compared to the main or lateral root. Malonyl ginsenoside contents in skin of root was higher than those of the main root without skin. These results is expected to help establish an efficient extraction and standardization. Malonyl ginsenoside analysis of White ginseng using HPLC expects that the standardization process can be established.

방사선에 의해서 유도된 간 손상에 대한 백삼과 발효인삼추출물의 보호효과를 비교 연구하였다. ICR계 생쥐에게 코발트-60 감마선의 5Gy조사 7일 전부터 백삼과 발효인삼추출물(150㎎/㎏/day)을 각각 투여하였 다. 대조군은 생리적 식염수를 투여하고 방사선조사군은 생리적 식염수를 투여하면서 5Gy를 조사하였다. 그리고 각각의 실험군에서 간조직의 H2O2, catalase, MDA를 측정하였다. 그 결과 방사선조사군과 보다 백 삼과 발효인삼추출물 투여군에서 catalase함량이 유의성 있게 증가하여 간의 보호효과가 있었으며 H2O2와 MDA함량도 유의성 있게 감소하였다. 백삼과 발효인삼이 간 조직에 대한 방사선조사로 부터 매우 우수한 방호제라고 할 수가 있다.

본 연구는 선형가속기의 고에너지 엑스선을 조사한 생쥐 간에 대한 백삼과 동충하초의 방사선방호효과를 연구하였다. ICR계 수컷생쥐 군에 7일 동안에 경구적으로 백삼(150 mg/kg/day)과 동충하초(200 mg/kg/day)를 각각 방사선조사 전에 투여했고 다른 생쥐 군에 5 Gy(1.01 Gy/min)의 방사선량으로 전신조사를 했고 대조군에 생리적 식염수 (0.1 ml)를 투여 한 후 간 조직에서 환원형 글루타치온(GSH)과 산화형 글루타치온(GSSG)의 함량을 각각 검사하였다. 그 결과 방사선조사군(Rad)보다 동충하초투여군(EF+Rad)과 백삼투여군(WG+Rad)에서 환원형 글루타치온 (GSH)함량이 유의성 있게 증가했으나 산화형 글루타치온(GSSG)의 함량은 유의성 있게 감소하였다. 총 환원형 글루타치온(total GSH)과 산화형 글루타치온(GSSG)의 함량 비율은 방사선조사군(Rad)보다 동충하초투여군(EF+Rad)과백삼투여군(WG+Rad)에서 유의성 있게 감소하였다.

The present study examined the effects of Korean white ginseng (WG, Panax ginseng C. A. Meyer) on the learning and memory function and the neural activity in rats with trimethyltin (TMT)-induced memory deficits. The rats were administered with saline or WG (WG 100 or 300 mg/kg, p.o.) daily for 21 days. The cognitive improving efficacy of WG on the amnesic rats, which was induced by TMT, was investigated by assessing the Morris water maze test and by performing immunohistochemistries on choline acetyltransferase (ChAT), acetylcholinesterase (AchE), cAMP responsive element binding protein (CREB) and brain derived neurotrophic factor (BDNF). The rats treated with TMT injection (control group) showed impaired learning and memory of the tasks, but the rats treated with TMT injection and WG administration produced significant improvement of the escape latency to find the platform in the Morris water maze at the 2nd and 4th days compared to that of the control group. In the retention test, the WG 100 and WG 300 groups showed significantly increased crossing number around the platform compared to that of the control group (p < 0.001). Consistently with the behavioral data, result of immunohistochemistry analysis showed that WG 100 mg/kg significantly alleviated the loss of BDNF-ir neurons in the hippocampus compared to that of the control group (p < 0.01). Also, treatment with WG has a trend to be increased the cholinergic neurons in the hippocampal CA1 and CA3 areas as compared to that of the control group. These results suggest that WG may be useful for improving the cognitive function via regulation of neurotrophic activity.