This study was conducted to investigate the effects of summer green manure crops as an alternative for imported expeller cake fertilizer on environment-friendly lettuce production. Sunnhemp, soybean, sudangrass, and sorghum, as summer green manure crops, were evaluated under the condition of a plastic film house. In the amount of nitrogen supplied through cultivation of green manure crops, soybean plot, which supplied 187 kg ha-1, was the highest, followed by sorghum plot, 156 kg ha-1. Since all green manure plots supplied more nitrogen than that required by soil testing, it was possible to replace the nitrogen supply of expeller cake fertilizer with the amount of nitrogen produced by green manures for autumn lettuce cultivation. N nutrient balances in sunnhemp, sudangrass, soybean, and sorghum plots were 13 kg ha-1, 28 kg ha-1, 30 kg ha-1, and 38 kg ha-1, respectively. The initial yield and total yield of lettuce showed no significant difference in green manure plots compared to the expeller cake plot, but . It was thought that all green manure crops could be substituted for expeller cake fertilizer used as nitrogen fertilizer, but it would be necessary to adjust the cultivation period and reduction point according to the type of green manure in order to maintain the C/N ratio, nitrogen supply, and nitrogen balance suitable for lettuce production.

The cultivation of green manure crops provide various beneficial influences on agricultural systems. However, there have been few studies on the use of green manure crops on upland field in Korea. The purpose of this study is the selection of green manure crop species suitable for subsequent upland crops. For this study, two leguminous green manure crops and four graminaceous crops were used as following: (1) hairy vetch (Vicia villosa Roth), (2) crimson clover (Trifolium incarnatum), (3) barley (Hordeum vulgare L.), (4) rye (Secale cereale L.), (5) triticale (Triticosecale wittmack), (6) wheat (Triticum aestivum L.). Plant-available nitrogen (PAN) release from each green manure crops was estimated using PAN prediction equation. Graminaceous green manure crops produced more biomass (dry plant matter) up to 1.5 times than leguminous crops. However, PAN % was much higher in leguminous crops due to their high nitrogen content (2.4 ～ 4.4%). In late may, hairy vetch was estimated to provide PAN as much as 11kg/10a which meet nitrogen need for nearly all (except waxy corn) upland crops used in this study. However, PAN from crimson clover and graminaceous green manure crops was much lower mainly due to low biomass production and low nitrogen content, respectively. Overall, hairy vetch could be most appropriate green manure crop for upland crop cultivation in Korea.

본 연구는 논토양에서 돈분액비의 시용이 화본과 및 두과 녹비작물의 생육과 양분공급량에 미치는 영향을 조사하기 위해, 화본과 녹비작물과 두과 녹비작물을 파종하여 돈분액비 시용유무에 따라 biomass 및 양분공급량을 조사하였다. 파종된 화본과 녹비작물은 호밀(Secale cereale L.)과 청보리(Hordeum vulgare L.)이었으며, 두과 녹비작물은 자운영(Astragalus sinicus L.)과 헤어리베치(Vicia villosa roth)이었다. 돈분액비를 투입함에 따라 녹비작물의 biomass는 유의성 있게 증가하였으며, 돈분액비 무시용구 대비 돈분액비 시용구의 biomass 증가율은 청보리(154%) > 호밀(138%) > 헤어리베치(45%) > 자운영(41%)순으로 높았다. 녹비작물의 양분공급량도 biomass와 마찬가지로 돈분액비를 투입함에 따라 유의성 있게 증가하는 경향이었으며, 돈분액비 무시용구 대비 돈분액비 시용구의 N, P2O5 및 K2O 공급량은 각각 205∼220, 109∼197 및 166∼184% 범위이었다. 돈분액비를 투입함에 따른 녹비작물의 biomass와 양분공급량 증가율은 전반적으로 두과 녹비작물보다 화본과 녹비작물에서 매우 우수한 경향이었다. 특히, 화본과 녹비작물의 증가된 양분공급량은 두과 녹비작물에 비해 약 3배 정도 더 높았다. 이상의 결과를 종합해 볼 때, 돈분액비 시용에 따른 양분이용효율은 화본과 녹비작물(호밀 및 청보리)이 두과 녹비작물(자운영 및 헤어리베치)에 비해 더 효과적인 것으로 판단된다.

연구는 당근-녹비 윤작지에서 헤어리베치 및 클림손클로버의 토양환원이 당근의 수량 및 토양의 물리․화학적 특성에 미치는 영향을 조사하였다. 당근재배지에서 전작물로 재배된 녹비작물의 질소(N), 인(P2O5), 칼륨(K2O), 칼슘(CaO) 및 마그네슘(MgO) 공급량은 헤어리베치의 경우 각각 23.8, 12.8, 6.1, 1.7, 및 2.8 kg 10a-1이었으며, 클림손클로버의 경우 각각 4.3, 6.0, 3.5, 1.3, 및 1.3 kg 10a-1이었다. 당근재배지에서 녹비작물의 토양환원 후 토양의 이화학적 특성을 조사한 결과 가밀도는 녹비작물 처리구가 대조구에 비해 감소하였고, 공극율은 증가하였다. EC는 녹비작물 처리구가 대조구에 비해 약간 낮았으며, 유기물, T-N 및 avail. P2O5는 대조구에 비해 높았다. 당근의 장해율은 녹비작물 처리구가 대조구에 비해 감소하는 경향이었고, 장해된 당근의 수량을 제외한 상품수량은 헤어리베치 처리구 (3,733kg 10a-1)가 대조구(2,566kg 10a-1)에 비해 45.5%의 증수효과를 보였다. 따라서 당근재배지에서 녹비작물의 시용은 비료로서 충분한 가치가 있으며, 녹비작물 토양환원 후 토양의 물리성 및 화학성을 개선함으로써 당근의 상품수량을 증대시킬 수 있을 것으로 판단된다.

Plant characteristics of four winter crop hairy vetch, red clover, crimson clover and rye as green manure were evaluated at three harvesting time(Apri1 23, May 9, May 28) in spring to select superior winter green manure crop. Rye and hairy vetch had super

Background: This study was conducted to investigate the effects of fertilization with the application of mixed organic matter after cultivating green manure crops on the growth and saponin content of Codonopsis lanceolata Trautv. Methods and Results: Five type of cultivation of green manure crops were done: hairy vetch as a single seedling crop (HV), hairy vetch and rye as a mixture of seedling (HV/R), additional fertilization with mixed organic matter after cultivation of a seedling mixture (HV/R/MO), chemical fertilizer (CF), and a non-treated control (NT). The total nitrogen content was the highest in the HV/R/ MO treatment, followed by the HV, CF, HV/R and NT treatments, respectively. Nitrogen fixation was higher in the single seedling treatment with hairy vetch (HV) than in the treatment with a mixture of hairy vetch and rye seedling (HV/R). Moreover, the growth of C. lanceolata was greatly increased by the application of additional fertilizer after treatment with the mixture of hairy vetch and rye seedlings. The HV/R/MO treatment produced the highest total lancemaside content among the treatments, followed by HV, HV/ R, CF, and NT, respectively. Conclusions: It was verified that C. lanceolata experienced superior growth with the application of mixed organic matter as additional fertilizer after the cultivation of green manure crops, and the content of its major saponins, lancemasides were also increased by this treatment.

Background : The organic matter content of land clearing or soil brought from an other place is very low, less than 0.5%. However, the organic matter content of natural habitat soil and leader farmhouse soil was two to three times higher than that of general farmhouse soil. As a result, the yield of Gastrodia elata was higher than that of general farmhouse. Therefore, this experiment was carried out to investigate the yield of G. elata according to the application of green manure crops (GMCs). Methods and Results : This experiment was carried out in a soil with organic matter content of 0.5% in rain shelter greenhouse. The kind of GMCs are corn, Sudan grass, sorghum and oats, and the throughputs per 10 a were 2,000 ㎏, 2,200 ㎏, 1,500 ㎏ and 700 ㎏ of each. The soil preparation was carried out after treated of GMCs at least one month before G. elata was formally planted. As the results of these experiment, organic matter content in soils increased 4 - 5 times compared with before treatment. As a result, the yield of G. elata increased by 21 - 41% in all treatments of GMCs. In addition, there was 2 - 5% more G. elata of good merchantable quality. However, the soil temperature in the summer season period of 2016 (7.1 - 8.31) was 1.2 - 1.6℃ higher than the previous year. The day when the outside temperature was above 30℃ was 41 days. It doubled from the previous year. Soil moisture content showed similar tendency according to treatments. As a result, 20 - 30% of high temperature damage and 20 - 50% of putrefaction occurred, and the total yield also decreased by 20 - 30% compared to normal year. Conclusion : In conclusion, some organic matter is needed for cultivation of G. elata. Therefore, it is considered that it would be better to keep the organic matter content at 2 - 3% when the destination are managed, and to use corn or Sudan grass as the GMCs.

Background:The study aimed to obtain data on the effects of cultivation and soil reduction of green manure crop on the quantity and quality of organically cultivated Cynanchum wilfordii Hemsley.Methods and Results:The experiment comprised four treatments: control, hairy vetch, barley, and hairy vetch + barley (3 : 2). The plant height in the hairy vetch treatment (86.3㎝) was significantly different from that in the other treatments, whereas the stem diameter leaf area, and special product analysis division (SPAD) value did not differ across the treatments. The largest soil reduction of green manure crop was recorded in the barley treatment (440 ㎏/10 a), whereas the smallest was recorded in the single treatment with hairy vetch (80 ㎏/10 a). The hairy vetch + barley (60 : 40) treatment showed 63% more soil microorganisms than control. Radical scavenging activity estimation revealed that the total polyphenol content was highest (1,740 ㎎/㎏), and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 92.6% in the barley treatment. The 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) activation was highest in the control (51.1%), and the root yield was the highest in the barley treatment (310 ㎏/10 a).Conclusions:The root yield, total polyphenol content, and antioxidant activity of Cynanchum wilfordii (Maxim.) Hemsley increased in presence of the green manure crop barley.

This research carried out to figure out the effect of the green manure crop cultivated at a preparation field and the shading net on the growth, development, and quality of ginseng. Followings are results obtained from the research. Leaf width of ginseng under the shading net of a two-layered blue and two-layered black polythylene net (TBTBPN) was good at rye and hairy vetch cultured group. Leaf length of ginseng under the shading net of a threelayered blue and one-layered black polyethylene net (TBOBPN) was good at barley and hairy vetch cultured group. Meanwhile, leaf width was good at hairy vetch cultured group. Leaf length of ginseng under a blue polyethylene sheet (BPS) was good at a barley and barley + hairy vetch cultured group, but stem length was shorter compare to other shading net cultivations. Root weight of ginseng was good under the shading net of a two-layered blue and two-layered black polyethylene net (TBTBPN) at a rye and hairy vetch cultured group, and was good under the shading net of a three-layered blue and onelayered black polyethylene net (TBOBPN) at a barley + hairy vetch cultured group, but there was no significant difference under blackout screen according to manure crop varieties. Ratio of rusty root was 10.2% at the barley cultured group under the shading net of a two-layered blue and two-layered black polyethylene net (TBTBPN), and was 23.1% at hairy vetch cultured group under shading net of a three-layered blue and one-layered black polyethylene net (TBOBPN). Ratio of rusty root was the lowest at a rye cultured group regardless the shading nets. Content of the ginsenoside was the highest at the rye cultured group under the shading net of two-layered blue and two-layered black polyethylene net (TBTBPN), was the highest at the barley cultured group under the shading net of a three-layered blue and one-layered black polyethylene net (TBOBPN), and was the highest at the rye cultured group under the blackout screen.

생강연작재배지 토양에 녹비작물의 재배가 토양 양분의 변 화와 뿌리썩음병원균 경감효과를 알기위해 헤어리베치, 크림 손클로버, 오차드글라스를 80일간 재배 후, 토양에 환원하여 토 양분석 및 real-time PCR 분석한 결과는 다음과 같다. 녹비작 물의 생육은 크림손클로버가 가장 좋았으며, 질수흡수량은 크 림손클로버가 가장 높게 나타났으며, 토양의 인산함량은 오차 드글라스가 가장 낮게 나타났다. T-N 함량은 헤어리벳치 > 크 림손클로버 > 오차드글라스 순으로 나타났다. 또한 토양 내 뿌 리썩음 병원균 밀도분석을 위해 Pythium zingiberum 균 특이 5.8S rDNA를 이용하여 real-time PCR 분석한 결과 헤어리베 치, 오차드그라스 및 크림손클로버 처리의 Ct값은 대조구보다 낮게 나타났다. 이상의 결과를 요약해볼 때 생강연작재배지에 서 녹비처리 후 토양은 대조구에 비해 EC가 감소되고, 몇 몇 무 기성분은 증가하였으며, 뿌리썩음 병원균 밀도는 감소하였다.

본 연구는 시설하우스 내 토마토 연작장해 경감을 위해 단기 녹비작물 재배가 토마토의 생육특성 및 수량에 미치는 영향을 알아보기 위해 토마토 시설재배지에 헤어리베치와 호밀을 단파 및 혼파로 구분하여 파종하였으며, 생육된 녹비작물의 양분공 급량과 녹비작물의 토양환원 후 토양의 이화학적 특성 그리고 녹비작물 환원 후 후작물인 토마토의 생육특성 및 토마토 시들 음 병원균의 밀도를 조사한 결과, 녹비작물의 질소(N), 인(P2O5), 칼륨(K2O), 칼슘(CaO) 및 마그네슘(MgO) 공급량은 헤어리베 치의 경우 각각 26.2, 5.8, 10.2, 6.6, 및 1.5 ㎏/10a였으며, 호밀 은 각각 9.1, 4.2, 11.8, 3.8, 및 3.1 ㎏/10a였고, 혼파의 경우는 단파와 유사한 경향이었다. 토마토 시설재배지에서 녹비작물 의 토양환원 전･후 토양의 pH는 대조구와 별다른 차이 없이 6.37-6.52 범위였으며, EC는 헤어리베치(2.64 dS/m) 및 호밀 (2.62 dS/m) 처리가 대조구(헤어리베치-2.80 dS/m, 호밀 -2.91 dS/m)에 비해 낮았다. 토양 중 유기물, T-N 및 avail. P2O5 함량은 녹비작물 처리가 대조구에 비해 증가하는 경향이 었다. 녹비작물 토양환원에 따른 후작물인 토마토 생육은 녹비 작물 처리는 대조구에 비해 우수한 생육을 보였으나, 관행처리 와는 비슷한 생육을 보였다. 또한 녹비처리에서 토마토 시들음 병원균의 밀도가 현저하게 감소하였다. 이상의 결과를 종합할 때, 토마토 시설재배지에서 녹비작물의 시용은 비료로서 충분 한 가치가 있으며, 녹비작물 토양 환원 후 토양화학성을 개선함 으로써 시설하우스 토마토 연작재배지의 연작장해를 경감할 수 있을 것으로 사료된다.

Stable production of green manure crop is one of the critical factor for successful implementation of environmental-friendly agriculture in South Korea. Most green manure crops including hairy vetch cultivated in South Korea must be passed cold winter season. This study was conducted to establishment of cultivation zones on green manure crops under paddy field in South Korea using on minimum temperature and drainage class. In order to build the spatial distribution map, hairy vetch, triticale, barley, rye, and clover as green manure crop were selected and mean of daily minimum temperature and drainage class in paddy field were considered as limiting factor for stable growth of green manure crop. And cultivation zone on green manure crops was divided into two categories; ‘Availability area’ and ‘Safety area’. Low limit temperature on green manure crops in this study was the following. Temperature on ‘Availability area’ was –10℃, –8℃, –6℃, and –20℃ for hairy vetch (including triticale), barley, clover, and rye respectively. And temperature on ‘Safety area’ was lower –2℃ compared to that of ‘Availability area’ exception rye which was the same degree. Rye was expected to safely cultivated the almost whole paddy field (99.71%) of South Korea. ‘Safety area’ among the whole paddy field for hairy vetch, barley, and clover were 86.58, 53.06, and 15.76% respectively. The spatial distribution map of green manure crops reestablished in this study could be used the policy and agricultural management for environmental-friendly agriculture using green manure crops in South Korea.

This study investigated ginseng growth and ginsenoside contents after control a reserved ginseng cultivation land using various green manure crops for stable ginseng cultivation. Followings are results obtained from this research. After cultivate the green manure crops, microbial flora in soil was diversified, organic matter and total-N content increased, but salt content decreased. The highest output obtained from the wheat cultivated area among various green manure corps. Growth of shoot and root of two years old ginseng increased significantly at the green manure crop cultivated area. Specially, the wheat cultivated area was the most effective in growth. Also, the rate of the leaf discoloration at the aerial part and the rusty root at the root was the least at the wheat cultivation area. Meanwhile, the ginsenoside content was the most at the wheat cultivation area. Thus, the reserved ginseng cultivation land could be managed by cultivating wheat for effective ginseng growth.

To improve the soil condition for no-tillage organic pepper cultivation, four different green manure crops were cultivated. Ferlilizer supply was depended on the biomass of the cultivated green manure crops, nitrogen supplies were 314kg in Vicia villosa and 341kg ha-1 in Vicia angustifolia. In the microbial community analyzed by phosph이ipid fatty acid (PLFA) method, soil microbe populations were different among the green manure crops and fungi group was increased at Vicia angustifloia and Vicia villosa. The bi이ogical ratio indexes of fatty acids in the soils, the ratio of Gramnegative to Gram-positive bacterial PLFA and Ratio of aerobes to anaerobes were high at Vicia hirsute and Vicia tetrasperma suggesting the enrich of the aerobic conditions. The ratio of saturated to unsaturated fatty acids increased at Vicia angustifloia and Vicia villosa suggesting anaerobic conditions. Abundant biomass and uncomposted organic matter, the ratio of fungi to bacteria was increased at Vicia angustifloia and Vicia villosa.

본 연구는 4종의 녹비 작물, hairy vetch, Italian ryegrass, crimson clover, orchard grass 등을 도입하여 연작장해를 일으키는 토마토 시설재배지의 토양개량에 미치는 효과를 구명하였다. 각각의 녹비 작물을 시용한 후, 후 작물로서 토마토를 정식하여 토양의 물리화학성 및 토마토 건물 중 등을 조사하여 녹비작물 재배의 효과 조사하였다. 그 결과 녹비작물 시용에 따라 토양 경도가 감소되었다. Orchard grass와 Italian ryegrass는 토양습도를 증가시키는 요인으로 토양 메트릭스에서 더 많이 무효화 시킬 수 있었다. 녹비작물 시용 2개월 후, Italian ryegrass와 orchard grass 처리구에서 토양 건조 밀도가 감소를 보였으나, 대조구와 녹비구간 통계적 유의성이 인정되지 않았다. 녹비작물로서 Italian ryegrass와 hairy vetch의 시용이 토마토 생육에 영향을 주어 건물중의 증가 및 양호한 생육을 보였다. 또한 orchard grass는 상대적으로 적은 바이오메스인데도 불구하고, 토양 개량에 효과적이었다.

This studies were conducted to investigate the growth responses and yields of Astragalus membranaceus Bunge which were grown with organic cultivation using two organic fertilizers and two green manure crops of hairy vetch and rye in 2008 and 2009. The lengths of stems and roots were not clearly different between organic and conventional cultivations. The root diameters and the branch numbers were slightly thicker and more in all treatments of organic cultivation than that of conventional cultivation in 2008, although they were not significantly different between two cultivating methods in 2009. The dry weights of shoot and root were heavier on organic cultivation than those on conventional cultivation. The T/R ratios were commonly higher in organic culture, showing more clearly in 2008 than 2009. The yields were generally higher in organic cultivation than those in conventional cultivation in 2008 although the effect did not clear on yield in 2009. The organic cultivation using of organic fertilizers and green manure crops would be new method to have safety and qualitative products. The techniques of organic cultivation for Astragalus membranaceus Bunge would be studied more on utilizing the natural organic resources.

최근 친환경농업과 화학비료의 가격 상승 등으로 인하여 녹비작물의 재배면적이 증가되고 있다. 그러나 중부지역에서 동계 월동이 가능한 녹비작물은 헤어리베치, 알팔파로 그 종류가 적어 이용이 어려운 실정이다. 따라서 녹바작물의 이용확대를 위하여 다양한 종류의 녹비작물을 선발하고자 봄에 두과녹비작물 8종을 밭토양에 파종하여 녹비의 이용 가능성을 평가한 결과는 다음과 같다. 녹비작물의 수량은 크림손클로버에서 ha당 생체중으로 23.6 ton, 건물중으로 4.16 ton이 생산되어 가장 많았고, 레드클로버, 루피너스, 자운영, 알팔파 순이었다. 또한 녹비수량은 모든 두과녹비작물에서 수확시기가 늦어질수록 증가하는 경향이었다. 작물별 질소 함량은 헤어리베치에서 kg당 41.3 g으로 가장 높았고 자운영, 네마장황, 화이트클로버, 레드클로버, 알팔파 순으로 32.2, 29.5, 29.2, 25.8 및 24.2 g이었다. 인산 함량은 알팔파(4.3 g kg1)와 크림손클로버 (4.2 g kg1)에서 높았고, 자운영, 레드클로버, 헤어리베치, 루피너스 순이었다. 칼리함량은 크림손클로버에서 35.9g kg1로 가장 많았고 레드클로버, 알팔파, 헤어리베치 순이었다. C/N율은 녹비작물 8종 수확시기가 늦어질수록 C/N율은 증가하는 경향을 보였으나 25 미만이었다. 두과녹비작물별 질소생산량은 녹비작물 8종 모두 수확시기가 늦을수록 증가하여 6월 19일에 가장 많았으며 작물별로는 크림손클로버에서 ha당 71 kg이 생산되어 가장 많았고, 자운영 (51 kg), 레드클로버 (46 kg), 헤어리베치 (41 kg) 순이었다. 따라서 녹비작물의 건물수량과 C/N율, 질소 생산량을 기준으로 볼 때 수원지역에서 봄에 파종하여 녹비로 이용 가능한 두과녹비작물은 크림손클로버, 자운영, 레드클로버, 헤어리베치가 우수하였다.

동작물과 하작물의 작부체계와 녹비작물을 활용하는 벼재배 기술을 개발하기 위하여 2007년과 2008년의 2년 동안 충북대학교 농업생명환경대학 농장 답작 시험포장에서 공시 녹비작물로는 두과작물인 헤어리베치와 화본과작물인 호밀을 사용하여 배 재배시 녹비작물들의 효과를 조사분석하기 위하여 수행한 연구결과는 다음과 같다. 초장은 최고분얼기, 수잉기에서 출수기에 이르기까지 완만한 속도로 컸으며, 관행처리구에서 가장 큰 약 105 cm정도를 보였고, 녹비처리구들에서는 약 100 cm 정도로 뚜렷한 차이는 아니지만 헤어리베치, 헤어리베치+호밀, 호밀처리구순으로 초장의 크기를 보였다. 최고분얼기의 분얼수는 녹비처리구에서 관행처리구에서보다 더 많았으며 헤어리베치와 헤어리베치+호밀처리구는 비슷하고 호밀처리구는 관행구와 비슷한 분얼수를 보였다. 출수기의 유효분얼수도 최고분얼기와 비교해 그 수가 줄었지만 비슷한 경향이었으며, 헤어리베치처리구에서 가장 많은 포기당 19개 정도였다. 벼의 수량 및 수량구성요소는 헤어리베치처리구가 관행구에 비해 주당수수가 뚜렷이 많았으며, 수수당 영화수도 2008년에는 관행구에서 많았지만 전반적으로 헤어리베치구에서 많았다. 천립중과 등숙율에서는 큰 차이를 보이지 않았지만 주당수수와 수수당 연화수에 의해 정조수량은 헤어리베치처리구에서 관행구에서보다 2007년에는 5.3% 2008년에는 7.9%의 더 높은 수량을 보였다. 반면에 호밀구에서는 관행구에 비교해 수량이 현저히 떨어 졌다.

참깨 연작장해 경감 녹비작물을 선발하기 위하여 9월 하순~10월 중순에 호밀, 헤어리벳치, 귀리 등을 파종 후 이듬해 5월 상순 환원하여 2004년부터 3년간 시험한 결과, 윤작에서 생육 및 수량이 양호하여 단일연작 48.9kg/10a 대비 33% 증수되었으며, 연작 간에는 녹비작물재배 시 양호하여 10~15% 증수되었고, 녹비작물재배 간에는 호밀재배에서 가장 양호한 경향이었다. 윤작 시 연작에 비하여 병발생, 토양의 시들음병균 밀도가 낮은 경향이었으며, 연작 간에는 녹비작물재배 시 양호한 경향이었다. 녹비작물재배 간에는 호밀과 헤어리벳치재배에서 양호하였고, 공극률은 윤작과 연작의 호밀재배에서 가장 높았다. 연작기간이 경과할수록 시들음병 및 역병 발생이 많았으며, 연작 간에는 호밀재배에서 가장 적게 발생되었고, 수량도 가장 많았다.