The yellow mealworm beetle, Tenebrio molitor L. (Coleoptera: Tenebrionidae), has long been used as a key study organism in many fundamental researches, including biochemistry, physiology, and behavior. Lifespan and reproduction are two of the most important components of fitness in all insects, but it remains largely unexplored how these two traits are influenced by macronutrient intake in this beetle. In this study, we used the nutritional geometry framework to analyze the complex and interactive effects of dietary protein and carbohydrate intake on lifespan and reproductive performance in T. molitor beetle. Lifespan and the number of eggs laid throughout the lifetime were quantified from more than 2,000 individual beetles provided with one of 35 chemically defined diets representing a full combination of seven protein-to-carbohydrate ratios (P:C= 0:1, 1:5, 1:2, 1:1, 2:1, 5:1, or 1:0) and five protein plus carbohydrate concentrations (P+C=25.2, 33.6, 42, 50.4, or 58.8 %, dry mass). All measures of lifespan and egg production were expressed highly at high caloric intake, but they differed in the optimal P:C ratio where traits peaked. While lifespan was the longest at a moderately carbohydrate-biased P:C ratio of 1:1.36, the rate of egg production was maximized at a protein-biased P:C ratio of 1.75:1, suggesting a possible nutrient-mediated trade-off between lifespan and daily reproductive efforts in T. molitor beetles. Lifetime egg production was maximized at a P:C ratio of 1.31:1, which was still protein-biased but lower than that maximized egg production rate. Reproductive lifespan was the longest at a P:C ratio of 1:1.06. When given a food choice, T. molitor beetles preferred a P:C ratio of 1:1, which is closest to the ratio that enables T. molitor beetles to stay reproductively active as long as possible.

Salt is crucial for survival, yet excessive intake of sodium chloride can have adverse effects. In the fruit fly, Drosophila melanogaster, internal taste organs located in the pharynx play a pivotal role in determining whether to accept or reject food. However, our understanding of how pharyngeal gustatory receptor neurons (GRNs) perceive high salt levels is still limited. In this study, we discovered that a specific member of the ionotropic receptor family, Ir60b, is selectively expressed in a pair of GRNs that respond to high salt concentrations. Through a two-way choice assay (DrosoX) to measure ingestion volume, we established that IR60b, along with two coreceptors, IR25a and IR76b, is necessary to deter excessive salt consumption. Interestingly, mutants lacking external taste organs but retaining internal taste organs in the pharynx showed significantly higher salt avoidance compared to flies missing all three IRs while still possessing all taste organs. These findings underscore the crucial role of IRs in pharyngeal GRNs in regulating the intake of high salt levels.

This study aimed to investigate the relationship between the nutrition quotient and the dietary intake of adolescents. A total of 393 adolescents were surveyed to evaluate their Nutrition Quotient for Korean Adolescents (NQ-A) scores and dietary intake. The average age of the survey subjects was 15 years and the average NQ-A score of the subjects was 49.11±13.35. There was no significant difference in the NQ-A scores according to gender and age. The average dietary diversity score was 3.77±0.85, and it was significantly higher in boys than in girls (p< .05) with the scores of 12-14-year-old students being significantly higher than those aged 15-18 years (p< .01). The results of comparing the percentage of recommended intake or adequate intake and the mean adequacy ratio (MAR) according to the NQ-A grade, showed that the ‘High’ grade had a significantly higher intake percentage of vitamin B1, B12, folate, phosphorus, iron and a significantly higher MAR (p< .05). From a long-term perspective, efforts to improve dietary habits are deemed necessary to meet an individual’s nutritional requirements. Adolescents themselves should develop proper eating behaviors and acquire suitable dietary management skills to enhance their nutritional status, ultimately contributing to an improvement in their quality of life.

본 연구는 8주간의 고강도 동계 훈련 시 L-아르기닌 섭취가 남자 대학 태권도 겨루기 선수의 경기수행능력, 젖산, 젖산탈수소효소 및 암모니아에 미치는 영향을 구명하기 위해 L-아르기닌 섭취군 (n=14), 위약군(n=14)으로 구분하여 실시하였다. L-아르기닌 섭취군은 일일 아침 1 g, 점심 1 g, 저녁 1 g으로 총 3 g 섭취하였고 위약군은 말토덱스트린을 동일한 방법으로 섭취하였다. 8주간의 동계 훈련 프로그램은 70-90%HRR로 실시하였다. 측정된 자료의 L-아르기닌 섭취군과 위약군 간의 그룹 및 시기 간 상 호작용 효과는 two-way repeated measures ANOVA, 그룹 내 시기 간 차이는 paired t-test를 사용하였으 며, 그룹 간 차이는 independent t-test를 사용하여 분석하였다. 그 결과 TAAA (Taekwondo-specific aerobic anaerobic agility) test를 통한 경기수행능력 중 평균 발차기 수에서 그룹×시기 간 상호작용 효과 가 나타났으며 그룹 간 주효과가 나타났다(p<.05). 또한, 발차기 피로지수에서 그룹×시기 간 상호작용 효 과가 나타났다(p<.05). 한편, 젖산에서는 시기 간 주효과가 나타났으며(p<.05) 젖산탈수소효소에서 상호작 용 효과 및 시기 간 주효과 나타났다(p<.05). 암모니아의 경우 그룹×시기 간 상호작용 효과가 나타났다 (p<.05). 이러한 결과는 남자 대학 태권도 겨루기 선수의 고강도 훈련 후 피로에 쉽게 노출되는 선수들에게 L-아르기닌 섭취로 인해 체내 피로 유발 물질들을 신속하게 제거하는 데 긍정적인 역할을 할 수 있다고 사료된다. 따라서 고강도 엘리트 태권도 겨루기 운동선수의 경기수행능력 향상과 피로 회복 방법으로 L- 아르기닌 섭취를 권장한다.

Radiation workers, especially those dealing with Uranium isotopes, can potentially intake Uranium -containing materials through their respiratory and digestive systems. According to the “Regulations on the Measurement and Calculation of Internal Exposure” from Nuclear Safety and Security Commission (NSSC), those who intend to work in or enter the nuclear facilities with a risk of exceeding 2 mSv exposure per year should be examined the internal exposure. However, when it comes to in-vitro bioassay, Uranium intake through drinking water can affect the quantitative analysis. The International Commission on Radiological Protection (ICRP) reported in ICRP Publication 23 (Report on the Task Group on Reference Man) that the reference man excretes Uranium in the urine (0.05-0.5 μg/day) and feces (1.4-1.8 μg/day). Korea Atomic Energy Research Institute (KAERI) set the 90.5 ng/day as the 238U background of workers handing Uranium based on the daily Uranium intake of Koreans. In this research, we examined the possible effects of Uranium in drinking water on internal exposure by analyzing the concentration of Uranium in bottled waters from various water sources sold in the domestic market and a water from the water purifier. The 238U concentration results of analyzing 11 bottled waters and 1 purified water, were ranged from 0 to 10.2 μg/L. All the results were satisfied the standard of 30 μg/L according to “Regulations for Drinking Water Quality Standards and Inspection” enacted by the Ministry of Environment. However, various concentrations were shown depending on the water sources. Assuming that these concentrations of water are consumed by drinking 1 L per day, the internal dose assessment result is 0 to 0.94 mSv. On the other hand, if it is assumed to be inhaled, it can be an overestimated because the dose coefficient of inhalation, Type M is higher than that of ingestion, f1=0.02 which are the values recommended by ICRP Publication 78 (Individual Monitoring for Internal Exposure of Workers) when the Uranium compound is unspecified. In case of two workers at KAERI, the daily excretion of urine was 151 and 120 ng/day respectively in the first quarter monitoring. However after changing the kind of drinking water in the second quarter monitoring, it dropped to 17.4 and 15.4 ng/day respectively. Through this study, it is confirmed that the Uranium background in urine can be analyzed differently depending on the kind of drinking water consumed by each worker. Depending on the Uranium concentration of drinking water, the internal exposure dose assessment can be overestimated or underestimated. Therefore, the Uranium concentration and intake amount according to the kind of drinking water should be considered for in-vitro bioassays of Uranium handlers. Furthermore, if necessary, the Uranium isotope ratio analysis in urine and the handling information should be comprehensively considered. In addition, in order to exclude the effect of intake through the digestive system, replacing the kind of drinking water can be considered. The additional analysis such as in-vivo bioassay and 24 hours urine analysis rather than spot samples can be also recommended.

Considering that the number of middle-aged single-person households is increasing, this study investigates dietary behaviors, nutrient intake, and mental health according to household type. Data were procured from the 2015-2019 Korea National Health and Nutrition Examination Survey (KNHANES). Totally, 5,466 participants aged 50-64 years were classified into 2 groups: a household with one member was defined as a single-person household, and households with two or more members were described as multi-person households. Single-person households comprised 10.63% of the total, with a higher average age, and lower income and economic levels than multi-person households. Compared to multiperson households, single-person households had a higher frequency of skipping breakfast, eating alone, and dining out, the moderately and severely food insecure group was more than 5 times, and nutrient intake and dietary quality were poorer. In the fully adjusted model, the odds ratios (ORs) of depressive symptoms were 2.35 times (95% CI: 1.39-3.96), and suicide ideation was 1.95 times (95% CI: 1.35-2.82) in single-person compared to multi-person households. Our results lead us to conclude that poor dietary intake in middle-aged single-person households affects the mental health, and the above factors should be considered when framing the dietary policy.

This study was performed to evaluate the pollutants removal characteristics of two types of RBFs(Riverbank filtration, Riverbed filtration) intake facilities installed in Nakdong River and in Hwang River respectively. The capacity of each RBF is 45,000 ㎥/d for riverbank filtration intake facility and 3,500 ㎥/d for riverbed filtration intake facility. According to data collected in the riverbank filtration site, removal rate of each pollutant was about BOD(Biochemical oxygen demand) 52%, TOC(Total organic carbon) 57%, SS(Suspended solids) 44%, Total coliforms 99% correspondingly. Furthermore, Microcystins(-LR,-YR,-RR) were not found in riverbank filtered water compared to surface water in Nakdong River. DOC(Dissolved organic carbon) and Humics which are precursors of disinfection byproduct were also reported to be removed about 59% for DOC, 65% for Humics. Based on data analysis in riverbed filtration site in Hwang River, removal rate of each contaminant reaches to BOD 33.3%, TOC 38.5%, SS 38.9%, DOC 22.2%, UV254 21.2%, Total coliforms 73.8% respectively. Additionally, microplastics were also inspected that there was no obvious removal rate in riverbed filtered water compared to surface water in Hwang River.

This study examined the determinants of healthy food purchases before and after COVID-19 in Korea. Binomial and multinomial logistic regression models were applied to Korea Rural Economic Institute's Food Consumer Behavior Survey data from 2019 to 2021. The analysis revealed a significant decrease in the non-intake of healthy food in 2021 compared to 2019, suggesting the impact of COVID-19 on healthy food consumption. Consumption patterns also changed, with a decrease in direct purchases and an increase in gift-based purchases. Several variables showed significant effects on healthy food intake. Single-person households exhibited a higher probability of eating healthy food after COVID-19. The group perceiving themselves as healthy had a lower likelihood of consuming healthy food pre-COVID-19, but this changed after the pandemic. Online food purchases, eco-friendly food purchases, and nut consumption showed a gradual decrease in the probability of non-intake over time. Gender and age also influenced healthy food intake. The probability of eating healthy food increased in the older age group compared to the younger group, and the probability increased significantly after COVID-19. The probability of buying gifts was significantly higher in those in their 60s, indicating that the path to obtaining healthy food differed by age.

본 연구는 제15차(2019) 청소년건강행태조사의 원시자료를 활용하여 생물-심리-사회 모델을 바탕으로 우리나라 청소년의 편의식품 섭취 경험의 영향요인을 파악하고자 하였다. 연구 결과 생물학적 요 인으로 성별, 학년, 아토피 피부염이 주요 요인으로 분석되었으며, 심리학적 요인으로는 스트레스 인지 수 준, 우울감 경험, 자살 생각 여부, 주관적 수면충족, 주관적 건강상태, 신체활동, 현재 흡연과 음주 경험이 영향요인으로 확인되었다. 또한 사회학적 요인으로는 영양 및 식이 교육, 아침식사 여부, 학업 성적, 주관 적 경제상태, 동거가족 여부가 청소년의 편의식품 섭취 경험에 영향을 미치는 것으로 나타났다. 이러한 결 과를 바탕으로 청소년의 건강한 식습관 관리를 위해서는 실효성 있는 영양 및 식이교육 프로그램 제공과 정서적 지지가 필요하며, 가족 구성원을 교육 대상에 포함시키는 등 다각적이고 체계적인 대책마련이 필요 하다.

This study focused on using indirect filtration through riverbeds to produce high-quality drinking water. Data on water quality from a water intake facility(capacity 10,000 m3/day) and nearby rivers were collected over a three-year period. The average intake facility specifications were found to be a specific surface area of 58 balls/m2, a mean particle size of 24 mm, an inflow velocity of 2.2 cm/sec, and a burial depth of 5 m. The water quality improvement rate was assessed as grade Ia, surpassing the adjacent river’s water quality. Correlation analysis showed a weak correlation between opening ratio, Suspended Solid (SS), and Biochemical Oxygen Demand (BOD) compared to total coliforms and fecal coliforms. The correlation coefficient R value of SS was -0.614, BOD was –0.588, total coliforms -0.870, and fecal coliforms -0.958. The R value shows a negative value, which showed that the larger the opening rate, the lower the removal rate of water pollutants. The correlation coefficient R values according to the depth of burial were found to be BOD 0.914, SS-0.124, total coliforms 1.000, and fecal coliforms 0.866. The deeper the burial depth, the higher the removal rate of BOD and microbial groups.

The labor environment in Korea has changed and problems related to employment types are treated as important issues. Especially, the ratio of irregular workers has not only increased relatively, but the labor conditions in Korea also have worsened in the current years. Studies have reported an association between temporary workers and the prevalence of diseases. However, there is insufficient research on chronic disease and employment types. Methods: The current study examines the prevalence rate of diseases and health behavior by categorizing employment types among Korean adults. Data were obtained from the 2016-2018 Korea National Health and Nutrition Examination Survey. Totally, the data of 2,366 workers (1,239 regular and 1,127 irregular) were analyzed in the study. The types of employment were classified by a questionnaire querying about working conditions. The results showed that irregular workers earned less than regular workers and had a significantly higher prevalence of diabetes mellitus and hypertension. We propose that if discrimination related to working conditions were relieved, irregular workers would be able to invest more time to exercise and doing check-ups regularly. In addition, individual nutrition consultations considering the knowledge and personal environmental factors of each individual are necessary for the improved health of all workers.

The objective of this study is to estimate the dietary exposure of polychlorinated dibenzo-p-dioxins and dibezofurans (PCDD/Fs) of Korean population via milk and meat using a probabilistic exposure assessment model. Total 319 raw milk and meat samples collected in the period 2006-2008 from nationwide Korea were measured the concentrations of 17 PCDD/Fs. Distributions of dietary exposure of 7 age subgroups to PCDD/Fs from the commodities were estimated probabilistically using Monte Carlo simulations. Dietary exposure groups were divided as lower, medium and high consumer subgroups according to the consumption of each commodity. The amounts of dietary exposures of Korean population subgroups were compared to the provisional maximum tolerable monthly intake (PTMI) recommended by the Joint FAO/WHO Expert Committee on Food Additives and Contaminants (JECFA). The mean PCDD/Fs concentrations in raw milk and meat of beef, prok and chicken were measured as 0.501, and 0.022~0.150pg WHO-TEQ/g, respectively. Dietary exposure of children was significantly higher than that of adults due to their high milk consumption per body weight (BW). Dietary intake of PCDD/Fs of the Korean populations estimated ranged from 0.154 to 1.248 pg WHO- TEQ/kg BW/day for high consumers (the 97.5th percentile) at the upper bound. Dietary intakes of average population of various subgroups were below the half of PTMI, but those of higher consumers were found exceeding or comparable to PTMI at the upper bound level. This study also suggests that the estimated PCDD/Fs concentrations in milk and meat are comparable to those reported in previous studies. Probabilistic assessment model for PCDD/Fs exposure in meat and milk commodities could be used to estimate the exposure of PCDD/Fs in Korean population for the development of risk mangement mesaures for PCDD/Fs in meat and milk.

목적 : 본 연구의 목적은 아동의 음식 섭취 시 시각 역할에 관한 연구 동향을 주제범위 문헌고찰 방법으로 확인하고 연구 방향을 설정하는데 기초자료를 제공하고자 하는 것이다. 연구방법 : 본 연구는 주제 범위 고찰을 통하여 1974년부터 2022년까지 국외에서 발표된 연구를 데이터 베이스인 CINAHL, MEDLINE, ProQuest, Science Direct에서 검색하여 본 연구목적에 적합하고 포함 및 제외기준에 해당되는 14개의 연구를 최종으로 선정하였다. 선정된 연구는 연구의 일반적 특성 및 연 구목적, 연구절차, 연구결과로 나누어 분석하였다. 결과 : 음식의 시각적인 요소와 섭취량과의 관련성 연구는 1990~2019년까지 꾸준히 증가하는 것으로 나 타났으며, 미국에서 가장 많은 연구가 이루어졌다. 연구대상자는 아동(2세 8개월~18세)을 대상으로 진 행하였으며, 이 중 유아 및 학령전기 아동만을 대상으로 한 연구가 6편으로 가장 많았다. 음식의 시각적 인 요소는 음식의 색, 모양, 크기로 분류되었으며 2000년대 들어서는 아동이 좋아하는 음식보다는 채소 와 과일과 같은 건강식품으로 시도하는 것으로 나타났다. 14편 중 10편이 해당 음식 섭취량을 증진하기 위한 목적으로 진행되었으며, 음식 섭취를 확인하기 위한 평가는 대부분 아동을 대상으로 대부분 임상 관찰로 진행되었다. 결론 : 본 연구 결과를 통해 음식의 시각적인 요소로는 음식의 색, 모양, 크기로 분류할 수 있었고, 이러한 요소들이 음식 섭취에 영향을 미친다는 것을 확인할 수 있었다.

This study investigated the association between dietary intake and 10-year risk for CHD predicted from Framingham risk score in Korean adults using the Korea National Health and Nutrition Examination Survey 2019-2020. Eight thousand subjects (3,382 men and 4,618 women) aged 30 years or older were classified into three groups according to the 10-year CHD risk (%). The sociodemographics, anthropometrics indices, blood profiles, and dietary intake were collected. After adjusting for confounding variables, the mean adequacy ratio (MAR) decreased for both men and women as the disease risk increased. The high–risk group was below the MAR criteria, indicating that the overall quality of the meal was not good. The highest consumers of legumes (OR 0.56, 95% CI 0.37-0.84), fruits (OR 0.67, 95% CI 0.48-0.93), and fish (OR 0.60, 95% CI 0.42-0.87) had a 44%, 33%, and 40% lower 10-year CHD risk than lowest consumers in men, respectively. In women, there is no significant relationship between food groups and disease risk. Therefore, improving lifestyle habits such as weight control, increased activity, and adequate food intake, especially legumes, fruits, and fish rich in antioxidant nutrients and bioactive substances, appears to have a potential association with preventing coronary heart disease in Korean adults.

목적 : 본 연구는 소아·청소년의 나트륨 섭취와 굴절 이상 사이의 연관성을 조사하였다. 방법 : 본 연구에서는 2016년 국민건강영양조사에 5~18세 소아·청소년 968명의 데이터를 사용하였다. 나트 륨 섭취량은 24시간 식단 회상에서 식품 항목에 대한 데이터로 측정하였다. 굴절 이상은 우안의 등가구면 굴절력 (SE)을 사용하였다. 잠재적인 교란 요인을 조정한 후 로지스틱 회귀 분석을 사용하여 나트륨 섭취와 굴절 이상 간 의 연관성을 분석하였다. 결과 : 나트륨 섭취량과 굴절 이상은 상관관계가 있었고(p<0.001), 나트륨 섭취가 높을수록 굴절 이상도 함께 높은 것으로 나타났다(p<0.001). 특히 나트륨 섭취량은 근시에서 유의하게 높았다. 잠재적인 교란 요인을 보정한 후 다변량 로지스틱 회귀 분석에서 높은 나트륨 섭취가 근시 위험 증가와 유의하게 관련이 있는 것으로 나타났다 (OR 1.62; 95% CI 1.12-2.33, p=0.010). 결론 : 국내 소아·청소년의 나트륨 섭취와 굴절 이상 사이에 유의한 연관성이 있는 것으로 나타났다. 또한, 높 은 나트륨 섭취량은 근시와 관련이 있는 것으로 나타났다.

본 연구의 목적은 남자 테니스 선수의 생맥산 섭취가 혈중 지질 및 동맥경화 지수에 미치 는 영향을 조사하는 것이었다. 남자 대학 테니스 선수 17 명을 4 주간의 고강도 테니스 하계훈련 중 생 맥산 섭취 여부에 따라 생맥산 섭취군(n=9)과 위약 대조군(n=8)으로 나누었다. 테니스 하계훈련은 4 주 간 주 5 회 실시하였으며, 운동강도는 예비심박수의 70~90%로 실시하였다. 생맥산은 아침 식사 전, 운 동 중, 운동 중, 운동 후 1 회 110ml, 저녁 식사 후 1 일 총 7 회 770ml 를 섭취하였다. 모든 데이터에 대해 평균 및 표준 편차를 사용하였으며, 시기간 및 생맥산 섭취그룹의 효과를 확인하기 위하여 반복 측정분산분석법을 사용하였고, 생맥산 섭취 후 혈중 지질의 차이에 대한 관련성을 알아보기 위하여 Pearson 의 상관분석을 실시하였다. 본 연구결과, 생맥산 섭취군은 혈중 지질(중성지방, 총콜레스테롤, 고밀도지단백콜레스테롤, 저밀도지단백 콜레스테롤)과 동맥경화지수가 유의하게 개선되었으며, ⲆTG, Ⲇ LDL 및 ⲆTC 간에는 유의한 상관관계가 나타났다. 결론적으로 남자 대학 테니스 선수의 고강도 트레이 닝 시 생맥산 섭취는 혈중 지질 및 동맥경화 지수에 긍정적인 영향을 미칠 수 있어 운동 보조제로서 효 과적인 스포츠 음료가 될 수 있음을 시사한다.

A pump-type eDNA filtering system that can control voltage and hydraulic pressure respectively has been developed, and applied a filter case that can filter out without damaging the filter. The filtering performance of the developed system was evaluated by comparing the eDNA concentration with the conventional vacuum-pressured filtering method at the catchment conduit intake reservoir. The developed system was divided into a voltage control (manual pump system) method and a pressure control (automatic pump system) method, and the pressure was measured during filtering and the pressure change of each system was compared. The voltage control method started with 65 [KPa] at the beginning of the filtering, and as the filtering time elapsed, the amount of filtrate accumulated in the filter increased, so the pressure gradually increased. As a result of controlling the pressure control method to maintain a constant pressure according to the designed algorithm, there was a difference in the width of the hydraulic pressure fluctuation during the filtering process according to the feedback time of the hydraulic pressure sensor, and it was confirmed that the pressure was converged to the target pressure. The filtering performance of the developed system was confirmed by measuring the eDNA concentration and comparing the voltage control method and the hydraulic control method with the control group. The voltage control method obtained similar results to the control group, but the hydraulic control method showed lower results than the control group. It is considered that the low eDNA concentration in the hydraulic control method is due to the large pressure deviation during filtering and maintaining a constant pressure during the filtering process. Therefore, rather than maintaining a constant pressure during filtering, it was confirmed that a voltage control method in which the pressure is gradually increased as the filtrate increases with the lapse of filtering time is suitable for collecting eDNA. As a result of comparing the average concentration of eDNA in lentic zone and lotic zone as a control group, it was found to be 96.2 [ng μL-1] and 88.4 [ng μL-1l], respectively. The result of comparing the average concentration of eDNA by the pump method was also high in the lentic zone sample as 90.7 [ng μL-1] and 74.8 [ng μL-1] in the lentic zone and the lotic zone, respectively. The high eDNA concentration in the lentic zone is thought to be due to the influence of microorganisms including the remaining eDNA.