The purpose of this study is to evaluate the effects of astaxanthin added to freezing buffer on semen parameters, total sperm oxidation stress after post-thawing of boar sperm and lipid peroxidation (LPO) which is caused by reactive oxygen species (ROS) in sperm membrane. Varying concentrations of astaxanthin (0, 10, 50, 100 and 500 μM) were used in the freezing buffer during cryopreservation to protect the DNA of thawed miniature pig sperm. Semen parameter was measured using computer assisted sperm analysis (CASA) for sperm motility and determine ROS rate, oxidative stress of boar sperm using fluorescence-activated cell sorting (FACS). Sperm motility was higher (p<0.05) in the astaxanthin group than in the control group. Sperm motility and the number of progressive motile sperm was higher (p<0.05) in the astaxanthin 500 μM group (66±1.7%) than in the control group (49.8±4%). In ROS evaluation, the astaxanthin group lowered intracellular O2 and H2O2 in viable sperm. The Yo-Pro-I/HE and PI/H2DCFDA staining as revealed using flow cytometry was lower in astaxanthin groups than in the other groups. As the result, we found that astaxanthin could protect the sperm plasma membrane from free radical and LPO during boar sperm post-thawing.

The present study was conducted to investigate the effect of different heights from liquid nitrogen (LN2) vapor on sperm motility and morphology after frozen-thawing. Two ejaculates were collected from 2 fertile Hanwoo bulls (A and B) by using artificial vagina at Hanwoo Research Institute. After collection, ejaculates were transferred to laboratory immediately and diluted with semen extender (Optixcell, France). Sperm dilutions were extended to a final concentration of 40 x 106 sperm/ml, and cooled at 4°C for 4 h and loaded to 0.5 ml straws. The straws were divided into 2 groups. Straws were placed in 3 or 9 cm of LN2 vapor for 14 min and then plunged into LN2 tank and cryopreserved until evaluation. Sperm motility and motility parameters (total motility, VSL with 25μm≥, VCL, VSL, VAP, LIN, STR, WOB, ALH and BCF) were evaluated by sperm class analysis (SCA, IVOS, Spain) after frozen-thawed. In bull A, 3cm group showed higher percentages of total motility, VSL with 25μm and VAP compared those with 9cm group (98.0 vs. 93.4%, 62.4 vs. 54.0% and 98.6 vs. 93.2%, 3 vs. 9 cm, irrespectively; p<0.001). In bull B, frozen-thawed sperm of 3cm group showed higher percentages of VSL with 25μm, VCL, VSL, VAP and BCF compared with those of 9cm group (43.5 vs. 26.0%, 123.8 vs. 111.6 μm, 62.9 vs. 57.3 μm and 81.5 vs. 72.5 μm; 3 vs. 9 cm, irrespectively; p<0.001). The viability and acrosomal integrity of spermatozoa were evaluated by Trypanblue/Giemsa staining method divided into 4 groups; live and intact acrosome (LIA), live and damaged acrosome (LDA), dead intact acrosome integrity (DIA), dead damaged acrosome (DDA). In bull A, frozen-thawed sperm of 3 and 9cm groups showed no significant difference in LIA, LDA, DIA and DDA. In bull B, 3 cm group showed higher LIA and lower DIA compared with those of 9 cm group (73.2 vs. 23.7% and 23.7 vs. 32.2%, 3 vs. 9 cm, irrespectively; p<0.001). We suspected that 3 cm vapor on LN2 vapor might be affected positively spermatozoa viability and acrosomal integrity compared with 9 cm group. In conclusion, semen freezing procedure in the present study will improve sperm quality after frozen thawing.

Cryopreservation of germ cells from genetically proven animals could be a source of restoration tools from the risk of extinction or disappearance of wanted characteristics. Using frozen semen, the genetic gains of Korean native cattle have been increased greatly for 70 years. The preservation of genetic resources as a form of frozen semen straw has limited availability due to the numbers. To circumvent this weakness of frozen semen, we tested two re-freezing methods with different initial thawing temperatures using frozen Korean proven semen and rare breed semen from albino, black and chikso breeders. It has been known that human sperm could resist to cryo-damages by repeated freeze-thaw cycles, but not for Korean proven bulls number (KPN) or for rare breeds. Total 7 frozen semem from brindled(2), black(1), Korean Albino(2) and KPN(1) bulls were used for our research. After thawing straws under 5°C/2min or 37°C/40sec with low temperature water bath and thermo jug, spermatozoa were re-diluted with triladyl diluents after first thawing and re-frozen. Sperm motilities were compared between animals and treated groups after re-thawing. Mean values of motility and viability of refrozen/thawed sperm for expansion of the number of straws were significantly higher in 5°C than in 37°C (P< < 0.05). However, the activity of viable sperm thawed at 5°C was significantly decreased after first and second thawing. It is estimated that re-freezing of frozen semen from rare Korean native cattle is possible with resistant properties of survived spermatozoa.

This study investigated the effects of 27.12 MHz radio frequency (RF) heating on heat transfer phenomena during the thawing process of frozen food. To determine the velocity of the RF thawing machine, samples were frozen at -80oC and subjected to different power treatments. The phase change times (-5 to 0oC) of frozen radish were 30, 26, 13, and 8 min; those of pork sirloin were 38, 25, 11, and 5 min; those of rump were 23, 17, 11, and 6 min; those of chicken breast were 42, 29, 13, and 9 min; and those of tuna were 25, 23, 10, and 5 min at 50, 100, 200, and 400 W, respectively. The heating limit temperatures of the radish, pork sirloin, rump, chicken breast, and tuna samples were 19.5, 9.2, 21.8, 8.8, and 16.8oC at 50 W; 23.5, 15.5, 27.3, 12.3, and 19oC at 100 W; 42, 26.9, 45.7, 22.1, and 39.4oC at 200 W; and 48.5, 54.7, 63.6, 57.3, and 44.9oC at 400 W. These results suggest that high-power RF improves thawing velocity and heating limit temperatures, and that an improvement on the operation of the RF thawing machine, according to food temperatures, is needed.

The conditions of blanching, freezing, and thawing for Shepherd’s purse (Capsella bursa-pastoris [L.]) were studied to improve its quality attributes during frozen storage. The washed samples were blanched with water at 100oC for 10, 20, 30, and 40 s. They were then soaked in cold water. The quality characteristics of the samples such as pH, texture, hardness, color value, and total viable cell were analyzed. The samples blanched for 30 s were frozen and thawed under several conditions and their physicochemical characteristics were analyzed. The pH value of raw samples was higher than that of the blanched sample. The values of brightness (L*), redness (a*), and yellowness (b*) decreased after blanching except the L* value of root at the condition of blanching for 40 s. Upon considering the bacterial cell counts, the proper condition was determined to be 30 s of blanching time. Blanching for 30 s was the optimal pre-treatment for minimizing the quality deterioration of the shepherd’s purse for freezing. Regarding its color change and microbial safety, faster freezing and thawing methods made less changes on the quality of frozen samples. Consequently, immersion freezing and radio-frequency thawing were the most effective in minimizing the quality change.

The recovery of epididymal sperm in animals is considered as one of the important tools to preserve high value or endangered species. However, there are no appropriate castrating indicators such as months of age in bull, sperm morphology, and motility, particularly in young Korean native bull (Hanwoo). Therefore, this study aimed to investigate sperm number, morphology, and motility of sperm in the epididymis tail of young Hanwoo bulls at 8 and 15 months of age. After castration, epididymal tails were collected and minced with blades to recover sperm. In experiments 1 and 2, sperm number, morphology, and motility were examined. Total number of sperm and percentage of normal sperm from bulls at 8 months of age was lower than that of bulls at 15 months of age after collection (P<0.05). Percentage of abnormal head, tail, proximal cytoplasmic droplet, dead and damaged acrosome of sperm from bulls at 8 months of age were higher than those of bulls at 15 months of age (P<0.05). In experiment 3, sperm motility from bulls at 8 and 15 months of age were examined before freezing and after thawing. Frozen-thawed sperm at 8 months of age showed low total motility and motile sperm with ≥ 25 μm/sec compared to those at 15 months of age and commercially-used sperm (P<0.05). In conclusion, sperm derived from the epididymal tail of bulls at 8 months of age showed high abnormal morphology and poor motility, which are not adequate for AI and IVF. On the other hand, sperm derived from the epididymal tail of bulls at 15 months of age showed high normal morphology and motility.

In the food industry, freezing storage has been an important process for maintaining the properties of food materials. In order to maintain the quality of blanched Colocasia esculenta (L.), Schott stem, packaging, freezing, and thawing methods were optimized by determinations of the physicochemical properties. For the comparison of packaging method, Colocasia esculenta (L.) Schott stem packed by air containment had the lowest significant differences of properties such as hardness and drip loss compared to the control samples. Overall, the drip loss of Colocasia esculenta (L.) Schott stem had lower value at fast freezing rate (immersion freezing). Considering the result of the drip loss, high frequency thawing was more effective than other thawing methods. Therefore, it was supposed that samples treated by air-containing packaging, immersion freezing, and high frequency thawing used the optimal method to maintain the original quality of Colocasia esculenta (L.) Schott stem.

Food freezing preservation is an important process due to its high quality and long shelf-life. In order to improve the quality of frozen sweet potato stem, the freezing rate, thawing, and packaging method was optimized by the determination of physical properties. There was no significant difference among the treatment of freezing rate. Further, the drip loss of sweet potato stem was higher at a relatively fast freezing rate (immersion freezing) than at slow freezing rate (natural convection freezing at -20 or -40oC). For the comparison of packaging methods, the aircontaining packaged sweet potato stem had the lowest significant differences from the result of color, pH, and hardness. From the result of the drip loss, the high frequency and microwave using thawing samples were more effective than room temperature and water thawing. Therefore, fast freezing and thawing rate with air-containing package were recommended to obtain the better quality of sweet potato stem.

The purpose of this study is to evaluate the quality characteristics of blanched Capsella Bursa-pastoris with quick freezing, and thawing methods. Blanched Capsella bursa-pastoris was packed by vacuum packing (VP) and air containing packing (AP). After then, they were frozen at -20, -40, and 60℃ (immersion freezing). Then, they were stored at -20℃ during 1 week. They were thawed with normal (room temperature), running water and high frequency method. The pH and color of Capsella bursa-pastoris were not different before and after freezing. Likewise, total bacterial counts were shown without variation among them. The amount of drip from thawed Capsella bursa-pastoris was generally more in VP than AP. The cutting intensity of immersion freezing wes higher than that of other freezing methods (P<0.05). Therefore, we selected AP, immersion freezing and high frequency thawing methods for the high quality according to above results

본 연구는 다양한 냉동과 해동처리에 따른 계육 가슴살에 natural microflora, 접종된 L. monocytogenes와 C. jejuni 수와 미세구조 변화 구명을 위하여 연구를 수행하였다. −20℃ 송풍식 냉동처리구의 총 호기성 세균과 C. jejuni 수는 4.06 log CFU/g과 4.09 log CFU/g으로 대조구와 비교하여 각각 약 0.7 log CFU/g과 1.0 log CFU/g의 감소를 보였다. 한편, 계육 가슴살 접종된 L. monocytogenes 수는냉동방법과 냉동온도에 따라 유의적인 차이를 보이지 않았다. 4℃와 25℃ 송풍식 해동처리구의 총 호기성 세균수는 3.70 log CFU/g과 4.02 log CFU/g으로 측정되어 대조구와 비교하여 각각 0.72 log CFU/g과 0.40 log CFU/g 감소한 반면 25℃ 유수식 해동처리구의 총 호기성 세균 수는 해동과정에서 균수가 급격히 증가하여 5.78 log CFU/g으로 관찰되었다. 해동 중 C. jejuni 수 변화는 해동방법보다 해동온도에 영향을 받는 것으로 나타났다. 냉동-해동반복 5회 후 총 호기성 세균과 효모 및 곰팡이 수는 감소하여 각각 4.15 log CFU/g과 2.30 log CFU/g을 보였다. 계육 가슴살에 접종된 L. monocytogenes 수는 냉동-해동 반복처리에 유의적인 영향이 없었지만 C. jejuni 수는 냉동-해동 반복 횟수가 증가함에 따라 감소하는 경향을 보였다. 냉동-해동 반복이 증가함에 따라 근섬유 조직을 재 손상시켰으며 특히 냉동-해동 반복 5회 후에는 계육 가슴살 시료의 조직세포 외부뿐만 아니라 내부에서도 공간이 발생하였으며 불균일하게 찢어진 세포가 관찰되었다.

본 연구에서는 다양한 냉동기법인 자연냉동 및 강제송풍 식냉동, 극저온냉동과 다양한 해동방법인 자연해동, 유수해 동, 초음파해동, 전자레인지해동을 통하여 물리적 변화를 최소화 하는 냉·해동공정을 찾으려 했다. 당근의 경우 품 질을 변화 시키는 요인은 해동방법보다는 냉동방법이 더 영향력이 있다. 해동감량 및 보수력의 변화를 관찰한 결과 급속냉동(강제송풍식냉동, 극저온냉동)을 한 경우 품질유지 에 더 효과적이었다. 경도는 냉·해동공정 보다는 블랜칭 처리를 한 것이(열처리) 유의적으로 품질이 저하되는 것을 관찰하였다. 즉 열처리가 당근의 물성에 영향을 더 미치는 것으로 판단된다. 색도 및 pH 경우, 냉·해동공정에 의해 크게 영향을 받지 않는 것으로 나타났다. 당근의 전체적인 품질을 최대한 유지 하기 위해서는 냉동법의 고려가 해동 법 보다 중요하며, 다양한 냉동방법 중 급속냉동처리하는 것이 효과적인 것으로 판단되며, 유지하고자 하는 당근 품 질 특성 별로 최적의 냉·해동공정을 선정하는 것도 효과적 이라 생각한다

The pre-treatment conditions of butterbur stem such as blanching, freezing, and thawing were studied to optimize it for producing frozen butterbur stem to improve its storage ability. Butterbur stems were hot water blanched at 100oC for 3, 5, or 7 min respectively and then soaked in cold water. After peeling out and cutting into blocks (4.5×1.0×0.5 cm), butterbur stems were air-dried for 5, 10, or 15 min respectively. Dried samples were analyzed for their physicochemical properties. With blanching, the hardness value increased from 2.91 kg to 3.64 kg (p>0.05); however, adhesiveness decreased drastically after 5 min of blanching (p<0.05), which changed to a crisp texture. In addition, considering other physicochemical properties, it was assumed that 5-min blanching was optimal pre-treatment to maintain the original quality of butterbur stem for freezing. Five-minute blanched butterbur stems were airdried 5 min, frozen and thawed with several methods, and analyzed for their properties. Considering the thawing loss, hardness, and color change, the fastest freezing and thawing method had the lowest changes on the quality of frozen butterbur stem. Therefore, to produce frozen butterbur stem, it was assumed that immersion freezing and running water or room temperature thawing (25oC) were the best process.

본 연구는 다양한 냉동방법(강제송풍식냉동, 극저온냉동, 일반냉동)과 해동방법(자연해동, 유수해동, 초음파해동, 전자레인지해동)을 조합하여 처리했을 때 표고버섯의 물리적 품질에 미치는 영향을 관찰하고, 표고버섯의 품질을 유지하는 가장 효과적인 냉·해동 공정을 탐색하는 것이 목적이었다. 급속냉동법(강제송풍식냉동, 극저온냉동)으로 처리한 경우 표고버섯의 해동감량, 보수력, 수분함량은 큰 변화 없이 품질을 유지 하는데 효과적인 것으로 관찰되었다. 특히 보수력은 저속냉동(일반냉동) 및 저속해동(자연해동) 처리의 유무가 영향을 미치는 것으로 나타났다. 전단력의 경우 냉동방법에 의한 영향보다, 전처리 및 해동방법에 더 영향을 받는 것을 판단된다. pH 및 색도는 냉동방법 및 해동방법에 따라 거의 차이가 없는 것으로 관찰되었다. 표고버섯의 물리적 특성을 효과적으로 유지 할 수 있는 처리 조합은 급속냉동(극저온냉동) 및 급속해동(유수해동, 초음파해동, 전자레인지해동)을 한 경우이며, 또한 유지하고 하는 물리적 특성에 따라 냉동 및 해동방법을 다양하게 조합하여 처리한다면, 식품의 품질을 보존하는데 더욱 효과적일 것으로 사료된다.

본 연구는 냉동식품 형태의 별미밥 HMR 개발을 위한기초 데이터를 확립하고자 냉동 및 해동 조건에 따른 취반미의 물리화학적 특성의 변화를 비교하였다. 본 실험에서는 냉동방법으로 강제송풍방식과 저온에서 자연적으로 냉동하는 방식을 사용하였으며, 냉동 취반미는 전자레인지의출력세기를 200, 600 및 1000W로 조절하여 시료의 중심부 온도가 100oC에 도달할 때까지 해동하였다. 분석결과 냉동속도는 일반냉동을 하는 것보다 강제송풍식 냉동을 하는 것이 약 300배 이상 빨랐으며, 해동은 동결 속도가 빠르고 전자레인지 출력이 셀수록 시간이 단축되었다. 냉해동한 취반미의 수분함량 변화는 모든 조건에서 대조구보다낮은 함량을 나타내었으며, 특히 600W에서 해동할 경우수분의 손실이 많은 것으로 나타났다. 취반미를 냉해동 한후 색도의 변화에 있어서는 대조구보다 백색도와 적색도는감소, 황색도는 증가하는 경향을 보였으며 강제송풍식 냉동을 한 취반미가 전체적인 색 변화가 적게 나타났다. 물성의 변화는 일반냉동을 한 취반미의 경도가 높게 나타났으며, 특히 600W에서 해동한 처리구가 높게 나타났다. 점착성의 경우에는 대조구보다 모든 처리구에서 감소하는 경향을 보였으나 조건에 따른 유의적 차이는 나타나지 않았다. 강제송풍식 냉동을 한 취반미의 미세구조는 냉해동 과정에서 밥알의 중심부에 균열이 발생하였고, 1,000W에서해동한 처리구에서 중심부에 비교적 큰 기공이 형성되었으며 600W에서 해동한 처리구의 경우에는 표면의 두께가가장 두껍게 나타났다. 냉해동한 취반미의 물리화학적 변화를 전반적으로 보았을 때 냉동 속도가 빠르고 해동시 전자레인지의 출력이 1,000W일 때 대조구와 비교하여 가장변화가 적은 것으로 나타났다.

본 연구는 최적의 냉해동 조건을 확립하고 설정된 조건에 따라 냉해동이 당근에 미치는 이화학적 분석 및 영양성분의 분석을 진행하였다. 본 실험에서는 강제송풍방식을이용하였으며, 자연대류식(0.2oC/min), 저속(0.4oC/min) 및고속(1.6oC/min)으로 냉동속도를 조절하여 -12oC까지 냉동하였다. 해동조건은 전자레인지를 이용하여 0, 200, 400,800W의 세기로 출력을 조절하여 시료의 중심부 온도가4oC가 될 때까지 해동하여 분석에 사용하였다. 당근의 강도는 냉해동 후 유의적으로 감소하는 경향을 보였으며, 특히 200, 400W 해동 시 냉동 속도가 빠를수록 감소하였다.해동감량의 경우 해동방법에 따른 유의적인 차이는 없었지만 냉동속도가 높을수록 감소하는 경향을 보였다. pH 및색도의 데치기 후 당근과 처리구간의 유의적인 차이는 보이지 않았다. 그러나 전자 현미경 관찰 결과, 냉동속도가빨라질수록 기공의 크기가 작아지는 것을 확인할 수 있었으며 이는 빠른 냉동속도가 식품 조직의 손상을 막는데 영향을 미친다는 것으로 보인다. 비타민 C 및 유기산(oxalicacid, malic acid 및 fumaric acid) 함량의 경우 유의적인차이는 나타나지 않았다. 유리당 함량의 경우에는 sucrose함량은 자연대류 냉동 시 가장 높았고, fructose 및 glucose의 함량은 유의적인 차이가 없었다. 본 연구 결과 냉해동방법에 따른 영양학적 변화는 대부분 데치기 후 당근과 유사하였으나, 당근을 고속으로 냉동할 시 물리적 변화 및조직 손상을 최소화 하였으므로 당근의 품질을 유지하는데효과적인 것으로 판단된다.

The aim of this study was to compare the effects of different thawing methods including; refrigerated at 4℃ for 6 hr, soaked in 15℃ water for 40 min, exposed to room temperature (25℃) for 4 hr, and microwaved (600 W) for 2 min on the water-soluble nutritional component and meat quality trait of Hanwoo beef. The thawing beef by the refrigerated method resulted in significantly lower cooking loss, drip loss and loss of water-soluble vitamin B group, whereas the water-soaked method had higher loss of vitamin B group (p<0.05). Furthermore, total aerobic counts and coliforms were significantly lower in the meat samples thawed by the refrigerated method than that in the samples thawed by the other methods when stored for 6 days at 4℃. However, the thiobarbituric acid reactive substance (TBARS) content was found significantly (p<0.05) higher in the microwaved method than that in the other thawing methods. Based on the results obtained in the present study, it is concluded that the thawing methods had remarkable impact on the water-soluble nutritional components, quality properties and storage stability of meat. Therefore, the present study recommends that the thawed meat needs to be consumed and should not be stored for long time due to the acceleration of its quality deterioration

본 연구는 Par-baked 미트파이 제조에 있어서 외피 특성과 굽는 조건 및 해동 조건이 Par-baked 미트파이 특성에 미치는 영향을 평가하였다. 미트파이 외피제조에 적합한 적정 가수량은 33%였으며, 적정 숙성시간은 한 시간이 적당하였다. Par-baked 미트파이를 위한 굽는 시간은 210℃에서 1차 굽기가 12분, 2차 굽기는 9분이 적당하였으며, 해동시간은 Par-baked 미트파이 특성에 영향을 미치지 않았다. 국내산 밀가루와 강력분이 중력분과 박력분에 비하여 Par-baked 미트파이 제조에 적합하였기 때문에 단백질 함량과 질적 특성이 빵용에 적합한 국내 밀 품종이 적합할 것으로 생각된다.