This study was conducted to determine the association between dietary calcium intake and biomarkers related to lipid and glucose metabolism and inflammation in Korean patients with type 2 diabetes. Seventy-five subjects (41 males, 34 females) were recruited from a group of patients who had visited the department of endocrine medicine. Data on anthropometric characteristics, clinical indices such as hemoglobin A1c and C-reactive protein (CRP), and dietary nutrient intakes were collected. Subjects were divided into three groups on the basis of their calcium intake [<EAR (below estimated average requirement), EAR-RNI (between EAR and recommended nutrient intake), >RNI (above RNI)]. Average calcium intake of <EAR, EAR-RNI, >RNI groups were 462.7±18.7, 649.7±12.8, and 895.7±21.7 mg, respectively. Energy intake was not different among groups but intakes of protein, total and saturated fatty acids were significantly higher in >RNI group than <EAR group. Analysis of covariance revealed that HDL cholesterol level was significantly higher in EAR-RNI group, as compared to <EAR group after adjustment with confounders such as age, sex, BMI and energy intake (p<0.05). Levels of CRP and homeostasis model assessment 2-insulin resistance (HOMA2-IR) were significantly lower in EAR-RNI group. Total cholesterol level was higher in EAR-RNI and >RNI groups, although within the normal range. Our results suggest that dietary calcium intake may influence the levels of HDL-cholesterol, CRP and HOMA2-IR and subsequently, help management/treatment of type 2 diabetes patients.
The nutritional compositions and physicochemical properties of two aronia varieties (Viking and Nero) cultivated in Chungbuk were investigated. The proximate compositions of Nero and Viking powder were:13% moisture, 0.58% and 0.6% ash, 0.73% and 0.33% crude lipid, 5.47% and 6.51% crude protein, 15.65% and 16.15% crude dietary fiber, and 64.36 and 64.43% crude carbohydrate, respectively. The proximate contents of the aronia samples were not significantly different. Among the physicochemical properties, there was also no significant change in total anthocyanin and antioxidant activity. However, Viking had higher total polyphenol contents and α-glucosidase inhibition activity than Nero. The mineral contents of Al, Fe, Mn, Mg, Ca, Na, K, Cu and Zn in the two aronias were determined. Cu and Zn were not detected in both varieties. With regard to Al, Na and K, Nero was higher than Viking, while Ca was higher in Viking than Nero. From the results, Viking could be suggested to be beneficial for food processing.
Recently, increased caffeine intake has led to an increase in caffeine addiction and withdrawal symptoms. Coffee is surreptitiously consumed in as an additive to milk and caramel. There are few studies on how coffee affects the health of modern people. The purpose of this study is to determine the efficacy and side effects of coffee by awareness of coffee consumption patterns among college students, who are the principal consumers. A survey was conducted from May 11 to 17, 2015 and 302 questionnaires were analyzed. The respondents were 140 men (46.4%) and 162 women (53.6%). In terms of coffee additives, 151 (50.0%) respondents chose ‘americano’ and 111 (36.8%) ‘variation’. The frequency of coffee intake and sleep time for college students was negatively correlated, with the correlation coefficient of -0.145 and significance probability of 0.019. The group that was ‘positively’ aware of the principal ingredients of coffee had a higher level of academic training than those with ‘negative’ awareness (p=0.000). Women recognized a larger number of side effects than men: 1.99 and 1.36, respectively, on average (p=0.001). ‘Time for consumption’ had statistically significant effects on the side effects of coffee consumption: consumption before/after lunch, before/after supper and before going to bed led to 0.4 times (p=0.048) and 0.3 times (p=0.023) more side effects, respectively, than consuming coffee after getting up and before/ after breakfast. Excessive caffeine intake through coffee led to limited sleep time and poorer learning concentration. The guidelines for proper coffee consumption should be created to help students consume coffee properly so that it will not affect sleep, learning concentration or adversely affect health.
Increasing sugar intake of population has become a nutritional issue in Korea. Sweet taste perception may be related to behaviors such as eating sweet food including high sugars and total sugar intake. This study aimed to evaluate objective and subjective sweet taste perception and the association among objective sweet taste perception, dietary behaviors related to eating sweet snack food including high sugar, and total sugar intake from the snacks. Participants were 261 healthy female college students (mean age: 21.0±1.6 years), who were divided into three subgroups based on oral sweet taste evaluation using a sweet taste assessment tool provided by Ministry of Food and Drug Safety: sweet-seeker group (n=139), medium sweet-seeker group (n=54), and unsweet-seeker group (n=68). There was no significant difference in weight and body mass index (BMI) among the three groups; however, the sweet-seeker group had significantly higher sweet taste preference than that of the other groups. Though more people in the sweet-seeker group thought they tended to eat sweet foods than the medium sweet-seeker and unsweet-seeker groups, over half of the sweet-seekers did not think they tended to eat sweet foods. The sweet-seeker group was more likely to eat sweet snacks such as breads, chocolate products, sugar-sweetened milk, and so on than the unsweet-seeker group. Total sugar intake from the selected sweet snacks was 44.4 g for the sweet-seeker group, 34.4 g for the medium sweet-seeker group, and 28.0 g for the unsweet-seeker group with a significant difference. These results indicated the absence of relationship between objective sweet taste perception and the obesity index; however, significant associations were detected among objective sweet taste perception, eating sweet snacks and total sugar intake from the snacks. We also found high disagreement between objective and subjective sweet taste perception of the subjects. The present study provided the novel insight that measuring objective sweet taste perception may be useful for assessing the risk of high sugar consumption and undesirable dietary behaviors.
The current study was conducted to facilitate appropriate hygiene and safety management in children’s food service stations, with the ultimate objective of providing sanitary and safe food service to children. In order to develop questionnaire items, literature review was conducted, in addition to detailed interview of working-level personnel at a children’s food service management support center. This resulted in a total of thirty questions on personal hygiene management, food materials hygiene management, and facilities hygiene management. Using the questionnaire, seventy-one food service stations for children in District A in Seoul that serve less than one hundred children were surveyed to analyze the reliability, construct validity, and correlation in the developed measurement tool. The developed measurement tool consisted of ten task and environmental hygiene management questions, five personal and cooking hygiene management questions, four food ingredient and storage hygiene management questions, and four food service operation and management question; with a total of twenty-three questions, in four factors stated above. The cumulative distribution of the four factors was 54.698%, and Cronbach’s α value was 0.672~0.853, which indicated that the study was reliable. The results of the analysis indicated that each of the factors were correlated, the study was satisfactory, and the tool was valid for evaluating hygiene and safety management practices in children’s food service stations. Finally, in order to enhance practical utility of the developed measurement tool, the significance and limitations were described.
In this study, the physicochemical and sensory characteristics of low-salt Myungran jeotkal (Alaskan pollock roe) were evaluated after fermentation at 4℃ and 20℃ with or without the addition of deep sea water, salt from deep sea water, and vegetable-origin lactic acid bacteria (Lactobacillus fermentum JS, LBF). When fermented at 20℃, the addition of LBF to Myungran jeotkal resulted in a slow increase in lactic acid content, followed by an abrupt increase after five days of fermentation. However, when fermented at 4℃, the lactic acid content did not change significantly. Further, when Myungran jeotkal fermented at 4℃, the pH decreased as lactic acid production increased. The salinity of Myungran jeotkal fermented at 4℃ and 20℃ was 7% and was not affected by fermentation period. When fermented at 20℃, volatile basic nitrogen and amino nitrogen contents increased with increasing duration of fermentation. Further, volatile acid content decreased, however, the content of amino nitrogen increased after 11 days of fermentation with LBF and no salt effects were observed. When fermented at 20℃ for 13 days, preference (sensory evaluation) was the highest in all experimental groups after 9 days of fermentation, and then decreased as the fermentation period increased. The free amino acid content was highest (1,648.8 mg/100 g) in Myungran jeotkal when sun-dried salt and LBF were added, 2.3 times higher than in the control.
The purpose of this study was to determine the optimum conditions of solvent extraction type and solvent concentration (60, 70, 80%, v/v), extraction time (30, 80, 130 mins) and extraction temperature (10, 15, 20℃) in order to increase the antioxidant activity of the chia seed. The total polyphenol content and DPPH radical scavenging activity was measured by using response surface methodology (RSM) to establish the optimal conditions. Using ethanol and methanol extractions at extraction concentrations of 60%, and time and temperature of 130 mins and 20℃, the maximum total polyphenol content was 871.00 mg% (R2=0.9507) and 557.70 mg% (R2=0.9784) for ethanol and methanol extraction respectively. Using the same extraction conditions, the maximum level of DPPH radical scavenging activity was 72.14% (R2=0.9675) and 52.79% (R2=0.9524) for ethanol and methanol extraction respectively. The results indicate that ethanol extracts showed a higher antioxidant activity than methanol extracts. The ethanol extraction conditions of response surface analysis (RSA) were affected more by ethanol concentration than by extraction time or temperature. In contrast, the methanol extraction conditions of response surface analysis (RSA) were affected more by extraction time. Based on the RSM, the optimum ethanol extraction conditions were the following: extraction concentration, 63%: time, 100 mins: and temperature, 18℃. The optimum methanol extraction conditions were the following: extraction concentration, 65%; time, 120 mins; and temperature, 16℃.
This study was undertaken to examine the knowledge about food culture and intakes of traditional food in Korean and Japanese elementary school students. In 2012 and 2013, a total of 265 students were surveyed, consisting of 73 Korean fifth graders and 192 Japanese sixth graders. The questionnaire comprised of queries on general items, the recognition and sampling of traditional and celebration foods, the knowledge about the partner country's food culture, and the frequency of consuming the partner country's foods. As a result of this study, the frequency of consuming their own country's traditional foods for Korean children (3.1 out of 5.0 points) and Japanese children (3.2 out of 5.0 points) was similar to each other. Japanese children reported to eat Korean foods (2.9 points) more frequently than those of Korean children to eat Japanese foods (2.4 out of 5.0 points) (p<0.001). However, the Korean children reported to have more experience on given typical Japanese foods than Japanese children reported about Korean foods. Further studies are needed for the perception on traditional foods in Korean and Japanese children. The results of this study can be used as a basic data to succeed and develop traditional food cultures.
The aim of this work was to select suitable fermentation treatments for the efficient bioconversion of cactus (Opuntia humifusa Raf.) bioactive components with an improved radical scavenging activity for use as a nutraceutical. To obtain microorganisms for the microbial conversion of cactus, Leuconostoc mesenteroides ATCC8294, Lactobacillus plantarum KCTC 3099, Lactobacillus plantarum KERI 236 and Monascus pilosus KCCM 60029 (ATCC 22080) were used for fermentation. Fermentation by Lac. plantarum KCTC 3099 was the most effective at scavenging 1,1-diphenyl-2-picrylhydrazyl hydrate (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals and reducing iron ( ). In particular, uronic acid levels showed a remarkable increase in fermentation. The polyphenol and quercetin content of the fermented cactus showed large increases from 108.65 μg/mL and 2.71 μg/mL to 227.83 μg/mL and 9.73 μg/mL, respectively, showing a maximum level at 36 h of fermentation with Lac. plantarum KCTC 3099. Thus, cactus fermentation with Lac. plantarum is an useful process for the enhancement of antioxidant contents and activity of fresh cactus.
The purpose of this study was to determine the optimal composite recipe of rice muffins with 3 concentrations of Spergularia marina L. Griseb, sugar and grape seed oil, using central composite design. In addition, the mixing condition of rice muffins was optimized by subjecting it to sensory evaluation and mechanical and physicochemical analysis using response surface methodology (RSM). In regard to its antioxidant effects, Spergularia marina L. Griseb had a total phenol and flavonoid contents and DPPH free radical scavenging activity of 17.03 mg/g, 5.13 mg/g and 17.21%, respectively. The results of mechanical and physicochemical analysis showed significant values for lightness, redness, yellowness, hardness, springiness, chewiness, gumminess, cohesiveness, height, volume, weight, specific volume, loss rate, pH, moisture, sweetness and saltiness (p<0.05), and the results of sensory evaluation showed significant values for color, flavor, taste, softness, appearance and overall quality (p<0.05). As results, optimal sensory ratio was found to be 6.69 g of Spergularia marina L. Griseb, 41.89 g of sugar and 30.48 g of grape seed oil.
This study was designed to develop and to qualify a coffee alternative beverage using a mixture of coffee beans and roasted black beans (Rhynchosia nulubilis). Therefore, the total isoflavone content (TIC), total phenol content (TPC), antioxidant activity, anti-inflammatory activity, NFATc1 (Nuclear factor of activated T-cells c1) expression in RANKL (receptor activator of nuclear factor kappa-B ligand)-stimulated RAW264.7 cells and sensory evaluation were measured for 5 different Cb (coffee bean)-RoS (roasted seomoktae) mixture extracts (Cb100RoS0, Cb75RoS25, Cb50RoS50, Cb25RoS75, and Cb0RoS100). Cb0RoS100 had the highest TIC (516.83±36.61 mg/100 g) and TPC (18.11±1.77 mg TAE/100 g) along with the highest antioxidant activity as measured by DPPH radical scavenging activity (73.55±8.11%) and ABTS radical scavenging activity (63.27±7.27%). Also, Cb0RoS100 showed the highest anti-inflammatory activity as measured by NO production (13.57±2.21 μM) and PGE2 production (3.25±0.21 ng/mL). The more the RoS ratio was increased in the mixtures of Cb-RoS, the more the NFATc1 protein expression was decreased in RANKL-stimulated RAW264.7 cells. In case of sensory evaluation, Cb50RoS50 had the highest scores for flavor, delicate flavor and overall quality, which were similar to those in Cb alone (Cb100RoS0). We suggest that the use of RoS replacement instead of Cb in/as a coffee alternative beverage may help to reduce the risk of caffeine-related bone loss and/or bone disease by effectively blocking NFATc1 expression in RANKLstimulated RAW264.7 cells compared with Cb alone.
The objectives of this study were to nutritionally evaluate Korean traditional fermentation food, Doenjang, and compare the nutrients in fish/meat-Doenjang and herb/fish/meat-Doenjang. In Doenjang, sugar, protein, fat, dietary fiber, and minerals, such as sodium and calcium, were analyzed using HPLC and the ICP analysis method authorized by the Korean Food Standards Codex. Doenjang is known to prevent arteriosclerosis, cancer and hypertension, and to aid liver function, as well as serving as a protein source. Doenjang, fish/meat Doenjang, and wild-herb/fish/meat Doenjang were made according to the original recipes in antique cookbooks. The highest levels of calories, carbohydrates, and sugar were contained in wildherb/ fish/meat Doenjang. Carbohydrate in the fish/meat Doenjang was higher than in ordinary Doenjang, and ordinary Doenjang had the lowest amount of sugar. Fat was shown to be the most abundant in fish/meat Doenjang, and the least abundant in ordinary Doenjang. Fish/meat Doenjang contained the most Na content, while wild-herb/fish/meat Doenjang had the lowest Na content. In terms of dietary fiber, ordinary Doenjang contained the most, while a wild-herb/fish/meat Doenjang contained the least. Ca content was shown to be most abundant in Doenjang, and the least abundant in fish/meat Doenjang.
The effects of dietary β-glucan, obtained from bacterial fermentation, on the intestinal mass, short chain fatty acids, lactate production and pH in Sprague-Dawley (SD) rats were evaluated. SD rats fed with 0% (control group), 1% or 5% β-glucan supplemented diets (w/w) for 3 weeks. The presence of β-glucan in the diets resulted in a significant increase in colonic contents in a dose dependent manner. The amount of short chain fatty acids increased in rats fed β-glucan diets. Rats fed the 5% β-glucan diets had higher levels of acetate, propionate and butyrate by 1.8, 1.7 and 3.0 fold of the control group in the cecum, and 2.2, 2.9 and 3.1 fold of the control group in the colon, respectively. The β-glucan diets also significantly increased the levels of cecal and colonic lactate by 1.4~3.4 fold, when compared to the control diet, indicating that dietary β-glucan stimulated the growth of lactic acid bacteria within the intestine. These results suggest that dietary β-glucan, by providing short chain fatty acids and reducing the cecal and colonic pH, may be beneficial in improving gut health, and provide evidence for the use of β-glucan as a dietary supplement for human consumption.
본 연구에서는 α-glucosidase 저해활성이 우수한 천연 자원을 개발하기 위하여 개똥쑥으로부터 천연 물질을 분리·동정하고 분리된 물질에 대하여 α-glucosidase 저해활성을 측정하였다. 개똥쑥 잎과 줄 기의 chloroform층과 ethyl acetate층으로부터 각각 6개와 5개의 화합물을 분리하였다. 분리한 화합물 중 chloroform층으로부터 분리된 ArteCA(M.W. 316g/mol, C16H12O7)는 isorhamnetin, ArteCB(M.W. 360g/mol, C18H16O8)는 chrysosplenol D, ArteCC(M.W. 316 g/mol, C16H12O7)는 rhamnetin, ArteCD (M.W. 374g/mol, C18H16O8)는 chrysosplenetin, ArteCE(M.W. 284g/mol, C21H20O12)는 acacetin 그리 고 ArteCF(M.W. 270g/mol, C16H14O4)는 imperatorin으로 확인되었다. 한편 EtOAc층으로부터 분리된 ArteEAA(M.W. 178g/mol, C9H6O4)는 6,7-dihydroxy coumarin, ArteEAB (M.W. 192g/mol, C10H8O4) 는 scopoletin, ArteEAC (M.W. 448g/mol, C21H20O11)는 kaempferol-3-O-b-D-glucoside, ArteEAD (M.W. 464 g/mol, C21H20O12)는 quercetin-3-O-b-D-glucoside 및 ArteEAE(M.W. 318g/mol, C15H10O8)는 myricetin으로 확인되었다. 이 중에서 ArteEAE(myricetin)는 α-glucosidase에 대하여 97.3%의 높은 저해활성을 가지고 있었으므로, 혈당조절용 건강식품 또는 치료제 개발을 위한 물질로 활용될 수 있을 것으로 판단되었다.
This study investigated the chemical composition of Solidago virga-aurea var. asiatica Nakai essential oils collected using different extraction methods. The essential oils obtained by simultaneous steam distillation extraction (SDE) and hydrodistillation extraction (HDE) methods from the aerial parts of Solidago virga-aurea var. asiatica Nakai were analyzed by GC and GC-MS. Ninety-nine volatile flavor compounds were identified in the essential oil produced from Solidago virga-aurea var. asiatica Nakai using the SDE method. These compounds were classified into eight categories in terms of chemical functionality: 26 hydrocarbons, 8 aldehydes, 36 alcohols, 7 ketones, 12 esters, 5 oxides and epixides, 4 acids, and a miscellaneous one. Spathulenol (15.66%) was the most abundant compound. Ninety-eight compounds including of 35 hydrocarbons, 6 aldehydes, 29 alcohols, 6 ketones, 10 esters, 4 oxides and epixides, 7 acids, and a miscellaneous one were identified in the essential oil from the plant using the HDE method. Hexadecanoic acid (24.74%) was the most abundant compound. The chemical composition of Solidago virga-aurea var. asiatica Nakai essential oils extracted by SDE and HDE methods are characterized by high content of sesquiterpene alcohols and acids, respectively. The extraction methods may be influenced in the chemical composition of natural plant essential oils.
This study investigated the changes in chemical components, antioxidant compounds, and activity before (BG) and after germinated (AG) brown rice in order to promote the availability of these beneficial factors. The GABA content of BG and AG brown rice were 0.07~6.61 and 11.13~49.72 mg/100 g, respectively. The α-amylase activity of AG brown rice was 1.77~70.25 unit/g fold higher than it was in BG brown rice. Total polyphenol and flavonoid contents, which are known to be related to antioxidation, were higher in BG brown rice than in AG brown rice. The total polyphenol contents of BG and AG brown rice were 10.52~36.38 and 11.38~26.33 mg/100 g, and the total flavonoid contents were 3.55~13.39 and 3.52~9.78 mg/100 g, respectively. Also, DPPH radical scavenging activity was 57.64~251.34 and 50.49~213.35 mg TE/100 g, respectively. ABTS radical scavenging activity and total tannin content showed a similar trend to DPPH radical scavenging activity. We expect that this data will be useful in the manufacturing of food products.
본 연구는 다류의 항산화 활성과 항산화 물질 함량에 대한 조사연구를 목적으로 하였다. 총 99건의 다류에 대하여 DPPH (1,1-diphenyl-2-picrylhydrazyl)의 자유라디칼소거에 대한 능력을 가지고 항산화 활성을 조사하였다. 항산화 활성은 차 시료 100 g당 아스코르빈산 당량의 mg(L-아스코르빈산 동등한 항산화 능력, AEAC)과 1회 제공량당 mg(자유라디칼 소거활성, FSC50)으로 나타냈고 시료추출액에서의 폴리페놀 함량은 Folin-Ciocalteu법으로, 플라보노이드의 함량은 Davis변법으로 측정하였고 Vitamin C는 HPLC법으로 분석하였다. AEAC 값은 마테차, 녹차, 홍차, 헛개차, 국화차, 우엉차에서 상대적으로 높은 항산화 활성을 보였다. 폴리페놀 물질은 차에서 자연스럽게 발생하는 중요한 항산화 물질로 홍차, 녹차 그리고 마테차에서 높은 함량을 나타냈다. 플라보노이드는 마테차, 홍차 그리고 마차에서, Vitamin C는 유자차와 녹차에서 높은 함량을 나타냈다. 유형별로는 침출차가 고형차나 액상차보다 높은 항산화 활성을 나타냈다. 연구결과, 다류에는 폴리페놀 함량과 항산화 활성사이에는 높은 상관관계가 있었고 플라보노이드와 항산화 활성사이에는 통계적으로 유의한 상관관계가 있었다. 즉, 다류의 항산화 활성은 폴리페놀에 의한 영향으로 확인되었다.
본 연구는 한국에 거주하는 동남아 노동자의 식생활 실태 를 분석하여, 한국에서 거주하는 동안 바람직한 식생활을 하 도록 지원하기 위한 기초자료 제시를 위해 조사되었으며, 조 사 결과는 다음과 같다. 1. 조사대상자의 연령은 25~29세가 35.1%로 가장 많았고 그 다음은 20~24세 (32.7%), 30~34세(21.1%)였고 35~39세가 9.6%로 가장 낮았다. 성별은 남성이 76.5%로 여성(23.5%)보 다 많았다. 결혼유무는 미혼인 경우가 59.8%였다. 교육수준 은 고졸이 49.8%로 가장 많았고, 다음으로 전문대졸(22.7%), 고졸 이하(17.1%)였고 대졸은 7.2%로 가장 적었다. 종교는 불교가 59.0%로 과반이 넘었고, 무교 35.3%, 기독교 4.0% 였다. 대다수가 공장(90.1%)에서 근무하고 있었다. 한국에는 4~5년 거주한 비율이 39.4%로 가장 많았고 2~3년 31.9%, 1 년 미만이 17.9% 순이었다. 대다수가 월평균 100~199만원 (88.0%)의 수입을 얻었다. 84.1%가 취사가 가능한 곳에서 거 주했다. 한국어 실력은 보통이 56.2%로 가장 많았고 친한 한 국친구 숫자는 한 명도 없다가 84.1%로 대다수를 차지하고 있고 그 다음은 15명이 12.7%였다. 2. FNS는 캄보디아, 미얀마, 베트남, 태국 순으로 높았다. FNS가 높은 캄보디아, 베트남과 미얀마는 각각 3.91±0.58과 3.92±0.65, 3.97±0.56로 평균 3.88±0.59보다 높았고 태국은 3.76±0.59으로 평균보다 낮은 지수를 보였다. 국가 간 유의 적인 차이는 없었으나 경제지표에 따라 경제가 발전한 국가 일수록 FNS는 낮아지는 경향성을 보였다. 3. FNS에 영향을 미치는 인구통계학적 요인은 취사가능여 부와 거주형태였다. 취사가능여부는 취사가능하다고 답변한 동남아노동자의 FNS (3.85±0.61)가 취사가능하지 않다고 답 변한 동남아노동자의 FNS (4.09±0.35)에 비해 낮게 나타났고 (p<0.05) 기숙사에 거주하는 동남아 노동자의 FNS (3.94± 0.55)가 자취(3.74±0.60)나 기타(3.83±0.66)의 FNS보다 높게 나타났다(p<0.05). 직접 취사를 하는 등 음식준비에 책임을 많 이 가질수록 음식관여도가 높아지고 높은 음식관여도는 FNS 와 부(−)의 관계를 가진다는 선행연구와 일치하는 결과였다. 4. 동남아 노동자의 한국음식인식의 유형에 따른 평균점수 를 중심으로 국가별 차이를 알아보기 위해 요인분석과 일원 변량분석(ANOVA)을 실시하였다. “한국음식의 조리법, 맛, 색에 대한 긍정적인 인식”은 캄보디아가 3.46±0.63으로 가 장 높았고 그 다음은 베트남(3.38±0.68), 미얀마(3.26±0.50), 태국(3.09±0.64) 순이었고 유의적인 차이가 있었다(p<0.01). “모국음식과의 조리법, 냄새, 질감에 대한 이질감” 요인에 대 해서는 미얀마가 3.40±0.60으로 가장 높았고 그 다음은 캄 보디아(3.16±0.76), 태국(2.88±0.98), 베트남(2.70±0.63) 순이 었으며 유의적인 차이가 있었다(p<0.001). “모국음식과의 맛 에 대한 이질감” 요인에 대해서도 미얀마가 2.84±0.79으로 가장 높았고 그 다음은 베트남(2.76±0.71), 캄보디아(2.65± 0.67), 태국(2.58±0.74) 순으로 높았으나 유의적인 차이는 없 었다. 비교국가에 비해 한국음식에서 모국음식과의 조리법, 냄새, 질감, 맛에 대한 이질감을 가장 많이 느끼고 있는 미 얀마의 경우 한국음식에 대한 적응이 쉽지 않을 것으로 예 상되며 이들에 대한 한국음식 적응 교육에 시간을 더 투자 하여 교육할 필요성이 있다. 5. 동남아 노동자의 한국음식인식정도와 FNS의 상관관계를 분석한 결과, “한국음식의 조리법, 맛, 색에 대한 긍정적인 인 식” 요인과는 p<0.01 수준에서 유의적인 부(r= −0.219)의 상 관관계를 보였고, “조리법, 냄새, 질감에 대한 이질감” 요인과 는 p<0.01 수준에서 유의적인 정(r=0.248)의 상관관계를 보였 다. “맛에 대한 이질감” 요인에서는 유의적인 차이는 없었다. 이 결과를 통해 FNS는 동남아 노동자의 한국음식인식정도와 연관이 있다는 것을 알 수 있다. FNS가 크면 “한국음식의 조 리법, 맛, 색”에 대해 더 부정적으로 인식하고, “조리법, 냄 새, 질감에 대한 이질감”을 크게 느낀다는 것을 알 수 있다. 한국음식에 대한 이질감은 맛(r=0.071)보다 조리법, 냄새, 질 감(r=0.248)에서 더 큰 이질감을 느낀다는 경향을 볼 수 있다. 본 연구를 통해 동남아시아 중에서도 유사한 지리적 특성 을 가진 인도차이나 반도에 있는 베트남, 태국, 캄보디아, 미 얀마 4개국 외국인 근로자들의 푸드네오포비아 경향은 국가 별 차이가 유의적으로 나타나지는 않았으나 경제가 발전한 국가일수록 FNS가 낮아지는 경향을 보였다. 한편 국가별 한 국음식인식에는 차이가 있다는 것이 나타났고 FNS는 한국 음식인식정도에 유의미한 연관성이 있다는 것이 밝혀졌다. FNS가 낮을수록 한국음식인식이 긍정적으로 나타났는데 “한 국음식의 조리법, 맛, 색”에 대해 더 긍정적으로 “조리법, 냄 새, 질감에 대한 이질감”은 더 낮은 수준으로 인식하는 것으 로 나타났다. 이 연구결과를 통해 동남아 노동자의 한국음식 적응에 대한 수월성 정도를 알아보기 위해 FNS가 선행적으 로 사용될 수 있을 것으로 기대한다. FNS가 높은 국가의 동 남아 노동자의 경우 입국 시 한국음식 적응을 위한 사전교 육에 시간을 더 할애하는 방법도 고려해 볼 수 있다. 국적 이외에 FNS에 영향을 주는 요인으로 취사가능성여부와 거 주형태였는데 음식준비에 대한 책임을 많이 가질수록 FNS 가 낮아지는 경향을 보였다. 따라서 FNS를 낮추기 위해 가 능하면 취사가 가능한 숙소를 배정해 적극적으로 취사를 할 수 있도록 유도하는 것도 음식문화 적응을 수월하게 하기 위 한 방법으로 사료된다. 한편 FNS는 한국음식인식을 판별할 수 있는 절대적인 지 표는 아닌 것으로 나타났는데 베트남의 경우 태국보다 FNS 가 높았지만 전반적인 한국음식인식은 태국보다 긍정적인 것 으로 나타났다. 태국은 타 동남아 국가 중에서도 음식문화가 발달한 나라로 자국민의 음식문화에 대한 자긍심이 높아 한 국음식인식이 비교국가 중 긍정적이지 않는 것으로 사료된 다. 따라서 한국음식 적응에 대한 수월성 정도를 알아보기 위한 사전조사로 FNS 뿐만 아니라 자국음식에 대한 자부심 정도를 알아보는 것도 필요할 것으로 보이며 이는 후속연구 로 진행되었을 때 의미가 있을 것으로 사료된다. 이 연구결과는 동남아노동자의 한국음식문화 적응을 돕기 위한 기초자료로 사용될 수 있을 것으로 기대한다. 또한 본 연구결과는 한국음식과 한국식품의 동남아시아 진출 시 필 요한 동남아인들의 한국음식에 대한 소비자 반응에 대한 기 초자료로도 사용될 수 있을 것으로 예상한다. 본 연구에서 연구되었던 4개국뿐만 아니라 다른 나라 노동자들에 대한 연 구가 추후 진행된다면 질 높은 기초자료 구축에 도움이 될 것으로 보인다.
본 연구에서는 소양인, 태음인 그리고 소음인 지원자의 혈액으로부터 분리한 면역세포에서 한국, 일본 및 미국에서 재배한 생마늘(RG)과 증숙마늘(SG) 70% 에탄올추출물에 대한 면역활성과 항산화능을 탐색하였다. 70% 생마늘추출물(RGE)은 세포 증식율, NO와 TNF-α 생성능이 소음인에 가장 높았고, 태음인과 소양인 순으로 나타났다. 반면에, 증숙마늘 추출물(SGE)은 각 개인별 체질에 영향을 미치지 않으면서 생마늘 추출물보다 면역활성이 더 높았다. 뿐만 아니라 국가별로 재배한 생마늘 및 증숙마늘의 면역효능은 나라별로 차이가 있었으며 한국산이 가장 높은 면역활성을 나타내었고 일본산, 미국산 순으로 나타났다. 이러한 결과는 항산화 효능에서도 유사한 경향을 나타내었다.
느타리버섯을 기능성 식품 소재로 활용하기 위해 에탄올 추출 농도에 따라 느타리버섯의 기능성 성분인 ergothioneine, total phenolic compounds 및 flavonoid 함량 측정과 항산화 활성을 시험하였다. 물 추출물(에탄올 0%)의 ergothioneine과 total phenolic compounds 및 flavonoid 함량이 각각 2.98 ± 0.05 mg/g, 9.51 ± 0.45 mg/g, 2.83 ± 0.03 mg/g으로 가장 높았고, 에탄올 농도가 높아짐에 따라 그함량은 감소하는 경향을 보였다. 항산화 활성을 측정한 결과 DPPH 및 ABTS 라디칼 소거능은 물 추출물에서 각각 80.41 ± 0.56%, 91.47 ± 0.11%로 가장 높은 활성을 나타냈고, FRAP 값도 물 추출물이 8.86 ± 0.33 FeSO4 eq. mM로 가장 높은 환원력을 나타냈다. 느타리버섯 추출물의 ergo- thioneine은 total phenolic compounds (r = 0.818)와 flavonoid (r = 0.837)와 높은 상관관계를 나타냈으며, DPPH (r = 0.614) 및 ABTS라디칼 소거능(r = 0.483)과 비교적 높은 상관관계를 나타내어 항산화성분 및 항산화활성과 관계가 있음을 확인하였다. 따라서 느타리버섯의 기능성 성분과 항산화 활성은 물로 추출하는 것이 가장 적합한 것으로 나타났다.