In the present study, we identified differentially methylated region (DMR) upstream of Dnmt1o and Dnmt1s gene in early porcine embryos. Porcine Dnmt1o had at least one DMR which was located between —530 bp to —30 bp upstream from transcription start site of the Dnmt1o gene. DNA methylation analyses of Dnmt1o revealed the DMR to be hypomethylated in oocytes, whereas it was highly methylated in sperm. Moreover, the DMR upstream of Dnmt1o was gradually hypermethylated from oocytes to two cells and dramatically changed in the methylation pattern from four cells to BL stages in an in vivo. In an IVF, the methylation status in the DMR upstream of Dnmt1o was hypermethylated from one cell to eight cells, but demethylated at the Morula and BL stages, indicating that the DNA methylation pattern in the Dnmt1o upstream ultimately changed from stage to stage before the implantation. Next, to elucidate whether DNA methylation status of Dnmt1s upstream is stage-by-stage changed in during porcine early development, we analyzed the dynamics of the DNA methylation status of the Dnmt1s locus in germ cell, or one cell to BL cells. The Dnmt1s upstream was highly methylated in one and eight cells, while less methylated in two, four, morula, and BL cells. Taken together, our data demonstrated that DNA methylation and demethylation events in upstream of Dnmt1o/Dnmt1s during early porcine embryos dramatically occurred, and this change may contribute to the maintenance of genomewide DNA methylation in early embryonic development.

본 연구는 유기재배 벼 시비에 있어서 주로 이용되는 유기자재가 토양화학성과 벼의 수확량과 품질에 어떠한 영향을 미치는 지를 구명하기 위해서 수행되었다. 전남지역 벼 유기재배에서 양분공급원으로 많이 이용되는 금수강산골드를 compost I으로 하였다. 식물성 성분이 많이 함유된 자재(쌀겨팰렛®)를 compost II, 생선가공부산물을 별도로 추가 제조한 자재(참달콤®)를 compost III, 그리고 동물성과 식물성이 혼합된 자재(한가위®)를 compost IV로 나누어서, 벼 이앙 후에 표층시비로 토양에 골고루 처리하였다. 유기자재내의 탄소 :질소율이 상대적으로 낮았던 compost I과 III가 compost II와 IV에 비교하여 시기별로 유기자재내 질소의 무기태화가 빠르게 진행되었다. 유기자재에 따른 토양의 화학성은 별다른 영향을 받지 않았고 토양의 시기별 유기물 함량은 유기자재 투입량이 많았던 compost II와 IV가 높게 나타났고 이는 biomass C에도 영향을 주었다. 처리에 따른 벼 수확량은 시용 첫해에는 compost I에서 높았지만 시용 2년차와 3년차에는 처리에 따른 차이는 나타나지 않았다.

The overexpression of Phosphoprotein Enriched in Astrocytes (PEA15) gene is commonly found in human diabetic patients. The overexpression of this gene in skeletal muscle and fat tissues have been reported to cause insulin resistance, thereby impairing insulin stimulated glucose uptake. We introduced a gene of mouse PEA15 (mPEA15) and enhanced green fluorescent protein (EGFP) into fertilized one cell pig zygotes using microinjection, and produced a piglet that showed overexpression of mPEA15 in the muscle tissues and expression of EGFP in the ear tissues and hooves. RT-PCR RFLP, southern blot and FISH analysis showed that the tissues carried the transgene. Real-time RT-PCR and western blots demonstrated that PEA15 gene was overexpressed in the various tissues and muscle tissues, respectively. These facts suggest that expression vector system is normally expressed in the trnasgenic (TG) pigs. To use as animal diseases model for type 2 diabetes, further study is necessary to confirm whether diabetes occur in these TG pigs, especially insulin resistance.

Deoxynivalenol (DON) and zearalenone (ZEN) are mainly contaminated mycotoxins in feeds. The study was carried out to analyze and survey the contamination of DON and ZEN in one hundred thirteen samples of feeds. After cleaning all samples with immunoaffinity column, the mycotoxins were analysed by using high performance liquid chromatography/fluorescence with diode array detector (HPLC /FLD with DAD). The average recoveries of DON were 88.76 and 95.40% at the levels of 200 and 1,000 μg/kg and 87.09 and 98.40% of ZEN were recovered at the levels of 100 and 500 μg/kg, respectively. The limit of detection (LOD) were 6.0 and 3.0 μg/kg for DON and ZEN, respectively. The average concentrations of DON were 372.1, 324.0 and 990.9 μg/kg in chicken, pig and cattle feed, respectively. Those of ZEN were 76.1, 43.7 and 196.2 μg/kg for them, individually.

This study aims to provide preliminary review for standardization of methods for evaluating reduction performance of pollution-reducing building materials and to select commercially available pollution-reducing building materials for assessing their current status. The ISO 16000-23 and -24 standards were used as references for standardizing the test methods. The sub test categories—stability of the supply air concentration, type of the supply air, and supply air concentration—which were not included in the standards were selected and conducted for the purpose of this study. Five (5) wall materials and 2 ceiling materials were tested for formaldehyde reduction performance and 3 wall materials were tested for toluene reduction performance. The study included experimental review of the essential test criteria such as stability of the supply air, internal stability of the chamber, and recovery rate. The samples tested exhibited some reduction performance for formaldehyde but little reduction performance for toluene.

포유동물 수정란의 동결보존기술은 최근 기후 변화에 따른 생물종 다양성을 보존하기 위해서 중요하게 여겨지는 연구 분야이다. 따라서 멸실 위험에 처한 동물의 개량과 증식, 보존과 복원 및 생명공학의 분야에 이르기까지 응용 기술은 다양하게 이용되어진다. 본 연구에서는 한우 수정란의 동결 후 생존성 향상을 위해서 동결 방법에 따른 체내 외수정한의 내동성을 조사하였다. 완만동결에 따른 체내 외수정란의 동결 융해 후 수정란의 재확장률은 89.6%와 81.5% 그리

비정형 초고층건물을 위한 구조시스템 중에서 다이어그리드 구조시스템은 구조적인 효율성 및 조형성 때문에 널리 사용되고 있다. 근래에는 초고층건물을 위한 구조시스템으로 메가부재의 조합을 통하여 횡방향 강성을 효과적으로 발휘할 수 있는 메가프레임 시스템이 널리 사용되고 있다. 두 가지 구조시스템의 장점을 혼합한 다이어그리드 메가프레임 구조시스템은 미래형 초고층건물에 적용될 유망한 구조시스템으로 평가받고 있다. 그러나 이러한 다이어그리드 메가프레임 구조시스템을 적용한 건물의 거동을 예측하기 위해서는 매우 많은 수의 절점과 요소로 이루어진 유한요소 모델을 해석해야 하므로 상당한 양의 해석시간과 엔지니어의 노력이 필요하게 된다. 따라서 본 연구에서는 다이어그리드 메가프레임시스템을 적용한 초고층건물의 거동을 효율적으로 해석할 수 있는 기법을 제안하여 다이어그리드 메가프레임 초고층건물의 해석과 설계에 소요되는 시간과 노력을 줄이고자 한다. 이를 위하여 다이어그리드 메가프레임의 특정을 활용한 효율적인 모형화기법과 행렬응축기법을 사용하여 해석에 사용되는 자유도수를 최소화한 해석기법을 제안하였다. 예제구조물의 해석을 수행하여 본 연구에서 제안된 해석방법과 일반적인 해석방법에 의한 결과와 비교함으로써 제안된 방법의 효율성과 정확성을 검증하였다.

To address global climate change, various governments are investing in electric vehicle research and, especially in Korea, the application of electric vehicles to public transportation. The lithium batteries used in electric vehicles typically have an exp

The purpose of this study was to assess the agreement of manual muscle testing (MMT) and test-retest reliability of a hand held dynamometer for the posterior gluteus medius muscle, with and without lumbar stabilization, using a pressure biofeedback unit for patients with low back pain. The pressure biofeedback unit was used to minimize the substitute motion of the lumbopelvic region during hip abduction in patients lying on their side. Fifteen patients with low back pain participated in this study. A tester determined the MMT grades of the posterior gluteus medius with and without the pressure biofeedback unit. Active hip abduction range of motion with an inclinometer and the strength of their posterior gluteus medius using a hand held dynamometer were measured with and without the pressure biofeedback unit in the MMT position. The agreement of the grade of muscle strength in the MMT, and intra-rater reliability of both the active hip abduction range of motion and the strength of posterior gluteus medius were analyzed using the weighted kappa and intraclass correlation coefficient (ICC), respectively. The agreement of MMT with the pressure biofeedback unit (weighted kappa=.92) was higher than the MMT (weighted kappa=.34)(p<.05). The inclinometer with pressure biofeedback unit measurement of the active hip abduction range of motion had an excellent intra-rater reliability (ICC=.90). Also, the hand held dynamometer with pressure biofeedback unit measure of strength of the posterior gluteus medius had a good intra-rater reliability (ICC=.85). Therefore, the test for muscle strength with pressure biofeedback unit will be a reliable method for the determination of the MMT grades or amount of posterior gluteus medius muscle strength and the measurement of the range of motion for hip abduction in patients with low back pain.

Although there have been various studies related to the body's movement from a sitting to a standing position (sit-to-stand task), there is limited information on the kinematic changes on the frontal and transverse planes. The purpose of this study was to ascertain how pelvic tilt affects kinematic changes in the frontal and transverse planes in the hip and knee joints during a sit-to-stand task. For this study, 33 healthy participants (13 female) were recruited. Each participant rose from a sitting to a standing posture at his or her preferred speed for each of three different pelvic tilt trials (anterior, posterior, and neutral), and the measured angles were analyzed using a 3-D motion analysis system. A one-way repeated measure analysis of variance was performed with Bonferroni's post hoc test. In addition, an independent t-test was carried out to determine the sex differences in hip and knee joint kinematic changes during the sit-to-stand tasks. The results were as follows: 1) The hip and knee joint angle in the frontal and transverse planes showed a significant difference between the different pelvic tilt postures during sitting in the pre-buttock lift-off phase (pre-LO) (p<.05). Compared to the posterior pelvic tilt posture, the anterior pelvic tilt posture involved significantly greater hip joint adduction and internal rotation, knee joint adduction, and reduced internal rotation of the knee joint. 2) Sex differences were found with significant differences for males in the initial and maximal angles in the frontal plane of the hip and knee joint (p<.05). Females had a significantly smaller initial abduction angle of the hip joint and a significantly greater maximal angle of the hip adduction joint. These results suggest that selecting a sit-to-stand exercise for pelvic tilt posture should be considered to control abnormal movement in the lower extremities.

본 연구에서는 주간동안 온실 내에서 발생되는 잉여 태양에너지를 분석하고, 또한 잉여 태양에너지의 적정 축열 시스템 설계에 필요한 기초자료를 제공할 목적으로 수행하였다. 분석에 이용된 기상자료는 표준기상년 데이터로서 이용하여 국내 주요 지역을 대상으로 온실 형태별로 잉여 태양에너지를 분석하였을 뿐만 아니라 소요 난방에너지 등도 분석 및 검토하였다. 이상의 결과를 요약하면 다음과 같다. 9개 지역을 대상으로 지역별 잉여 태양에너지를 대해 분석한 결과, 난방에너지 대비 잉여 태양에너지 비율은 온실 형태별로 각각 약 212.0~228.0%로서 제주가 가장 높게 나타났다. 그 다음으로 부산, 광주, 진주, 대구, 대전, 전주, 수원, 및 대관령 순으로 나타났다. 그리고 온실 형태에 관계없이 몇 몇 지역을 제외 하면 잉여 태양에너지만으로 소요 난방에너지를 거의 대체할 수 있을 것으로 판단되었다.

A survey of consumer awareness and attitudes was conducted about genetically modified (GM) foods and the labeling regulations. The questionnaires were distributed to 4,620 consumers who lived in a variety of areas in Korea, and 4,076 people responded. The consumers were asked about knowledge, labeling information, and the source of obtaining information about GM foods. More than 11.5% of the consumers had never heard about GM foods and 86.9% of consumers had less than a normal level of knowledge about GM foods. No statistically significant relationship was found between genders, but the teachers group had moderate knowledge (p<0.001). In total, 28.4% of consumers did not know the GMO labeling regulations. They answered that the reason to buy GM food was do not know>nothing wrong>create benefit>think as safe>inexpensive. The answers to the question of what was the first benefit were: solve food shortage>functional and nutritious food>cultivate in bad condition>nothing>various cultivars. They answered that the worst factor was the next generation effect>environmental disruption. Regarding the development of GM food in Korea, males answered do not know>stronglyrecommend>defer>strongly suppress. Female answered: don't know>defer>strongly recommend>strongly suppress. More than half of the respondents did not have much information about GM foods; 88.3% of respondents answered they did not have educational experience about GM food.

Optimization study of the hot water extraction for enhancing antioxidative activity from Auricularia auricula was performed by Taguchi approach using orthogonal matrix L9(34) method. The correlation between DPPH radical scavenging activity and the components of samples extracted from different extraction conditions were also analyzed. The correlation coefficient between DPPH radical scavenging activity and melanin content of A. auricula was 0.93, indicating 'good correlation'. The optimum extraction conditions were obtained at the extraction time of 1 hr. temperature of 85oC, solid: water ratio of 1: 40(w/v) and frequency of 2 times. Under these conditions, values of maximum DPPH free radical scavenging activity and melanin contents of A. auricula were 67.21±2.17 and 52.94±2.10 mg/g, respectively. Melanin content of 1.6 times and DPPH free radical scavenging effect of 130% were enhanced by optimization.

Physiological characteristics of brown rice and black rice were investigated to provide the fundamental information of physiological property of rice and to show the potential of rice as a functional ingredient. Bioactive compounds were extracted from brown and black rices with aqueous solvents like 80% ethanol. Total phenolics, flavonoids and antioxidative capacity of brown and black rices’ extracts were determined. Brown rice showed much higher amount of total phenolics and flavonoid contents as well as antioxidative capacity than those of milled rice indicating that most of bioactive compounds are located in the bran layer. Black rice showed higher total phenolics and flavonoid contents and antioxidative capacity than those of brown rices. The highest antioxidative capacity was obtained from Heugjinju followed by Heugseol, Sinnongheug-chal, Hopum and Samkwang. This result indicated that antioxidative capacity is affected by total phenolics and flavonoid contents. Both brown and black rices contained higher amount of ferulic acid than that of p-coumaric acid.

The purpose of this paper is to research Gunnar Asplund's Woodland Chapel (1918-20) in terms of the revernacularization of classicism and to investigate into the matter of the constructional logic. The term 'revernacularization of classicism' was used by Alan Colquhoun to explain the process to return to the pure sources of classical architecture, and the case of a successful fusion of classicism and vernacular traditions was suggested by Demetri Porphyrios through Scandinavian Doricist sensibility in the early 20th century. Porphyrios's classicism, not as style but as sensibility, is premised on a constructional logic of vernacular, and is to achieve an aesthetic quality by its mythical elaboration. Woodland Chapel, a representative of the Scandinavian Doricism according to him, illustrates characteristics of the revernacularized classicism as in the fact that it thickly displays vernacular images at the same time as relying on classicism; in the return to primitive simplicity; and in the mythopoeic power. However, the constructional logic of this building was obscured in the capital of the portico columns, the interior dome, and the whole structure of the roof. Confronting this paradox, we have to remember that although Porphyrios emphasized the constructional logic he opened an aesthetic exit of the mythical elaboration, which is in accord with the concept of the tectonic as the poetics of construction. Woodland Chapel assumes atectonic features but is never anti-tectonic. Asplund intensified a poetic effect by setting the myth over construction in the chapel, and so it can be seen as a key example of the revernacularized classicism with the Doricist sensibility.

본 연구는 허브 부식토를 이용하여 첨가 수준별 in vitro 반추위 발효특성 평가와 젖소를 이용하여 급여시 유생산성에 미치는 영향을 조사하기 위하여 본 연구를 수행하였다. 시험 1에서는 티머시 건초를 기질로 하여 허브부식토(herbaceous peat)를 0,1 및 5%를 3반복으로 각각 첨가하여 in vitro 반추위내 pH, 가스발생량, VFA (volatile fatty acid), ammonia-N 및 건물분해율을 조사하여 반추위내 발효성상의

본 연구는 유기재배 배 과수원 시비에 있어서 화학 비료 대체를 위한 녹비작물 재배 이용기술 개발을 확립하고자 호밀과 헤어리베치의 파종시기에 따라 녹비작물의 생육과 양분 공급량에 미치는 영향을 구명하기 위해서 수행되었다. 녹비작물의 파종시기는 2008년 9월 27일, 10월 15일, 11월 8일, 12월 10일로 나누어서 전남 보성의 배 과수원 독농가에 처리하였다. 관행 방임 초생 재배구는 대조구로 선정하였다. 호밀의 생육은 파종시기에 따라 별다른 차이 없이 비슷한 경향이었으나 헤어리베치는 파종 시기가 늦을수록 초장이 짧아지고 엽수와 줄기수가 감소하였고 건물중에도 그러한 비슷한 경향이 관찰되었다. 9월 하순 녹비처리는 관행 방임 초생 재배구에서 생산한 362kg/10a의 건물 중 보다 2.6배 높았다. 파종시기별 전질소 농도는 파종 서기가 늦을수록 증가하는 경향이었으며, 전질소 환원량은 피종 시기가 빠른 9월 하순처리가 16.9k/l0a으로 관행대비 2.6배 높았다. 인산 환원량은 10월 중순 파종이 가장 높았으며, 칼륨 환원량은 파종 시기가 빠를수록 증가하는 경향을 나타내었다.

2010년 영산강 섬진강 수계의 29개 호소 45개 지점의 환경조사에서 동정된 식물플랑크톤은 총 371종으로 규조강과 녹조강이 주를 이루었다. 호소의 규모와 식물플랑크톤의 분포 양상 사이에 상관성이 거의 없는 것으로 나타났다. 호소의 유역면적과 식물플랑크톤 현존량은 상관성을 보이지 않았고, 유역면적과 출현종수 사이에도 상관성이 매우 낮았다. 수표면적과 식물플랑크톤 출현종수와 현존량 사이에는 낮은 상관성이 나타났다. 본 조사 호소들에서 호소의 규모와 식물