유류와 위험 유해물질(Hazardous and Noxious Substances, 이하 HNS)을 포함하는 위험물질의 해상운송 중에 발생하는 사고가 증가하고 있어, 이러한 위험물질 해양사고를 방지할 수 있는 예방대책을 마련해야 할 것이다. 이를 위해 국내에서 발생하는 위험물질 해양사고 자료(2002~2014)를 수집하여 인명 및 환경 위험도를 분석하였으며, 사고원인 규명 및 적절한 예방대책이 필요한 우선 안전관 리분야를 파악하고자 하였다. 인명위험도는 선박 수리 및 탱커세정(tank cleaning) 작업과정에서 발생하는 폭발 및 인명사고 중 질식 등 이 높은 것으로 분석되었다. 환경위험도는 충돌, 좌초 등에 의한 유출사고로 인해 발생하였다. 또한 비포장 상태의 위험화물을 선박에 적재 중 취급부주의로 인하여 유출사고가 일어났다. 다수의 사고는 적합한 안전장비 미착용, 취급 부주의 등 인적오류로 인한 사고로 서 관리·감독을 강화하고, 주기적인 교육·훈련을 통하여 사고 저감이 가능하다. 위험물질 해양사고를 예방하기 위해서는 물질 자체 고 유의 특성상 유해성과 위험성을 지닌 위험물질(유류와 HNS)에 대한 안전관리를 더욱 강화해야 한다.

In this study, we investigated the effect of bisphosphonate on the osteoblastic differentiation of human dental stem cells (hDPSCs). In the first experiment, we evaluated the effect of bisphosphonate on the differentiation of hDPSCs into osteoblasts by alkaline phosphatase staining after culturing hDPSCs. As a result, on day 13, the osteogenic differentiation of hDPSC was suppressed at 5 μM in clodronate and 2 μM in zolendronate. In NBP, osteogenic differentiation is more suppressed. In second experiment, cytotoxicity and proliferation test, the cell proliferation (examined by MTT assay) was more suppressed as the concentrations of zolendronate were larger than those of alendronate and clodronate. Western blotting, a third experiment, was found that AKT phosphorylation was inhibited in cell signaling proteins involved in cell proliferation inhibition and death by bisphosphonate concentration. In human dental stem cells, bisphosphonates inhibit osteoblast differentiation, and this phenomenon is clearly observed in NBPs (zolendronate), and it has been found that it is related to AKT phosphorylation of cell signaling proteins.

In vivo assay of glucose detection was described using a skin tattoo film electrode (STF), and the probe was made from carbon nano tube paste modification film paper. Here in the square-wave stripping anodic working range obtained of 20-100 mgL-1 within an accumulation time of 0 seconds only in sea water electrolyte solutions of pH 7.0. The relative standard deviations of 50 mg glucose that were observed of 0.14 % (n=12), respectively, using optimum stripping accumulation of 30 sec, the low detection limit (S/N) was pegged at 15.8 mg/L. The developed results can be applied to the detect of in vivo skin sensing in real time. Which confirms the results are usable for in vitro or vivo diagnostic clinical analysis.

China’s sole nationality principle was formulated at the beginning of the People’s Republic of China. However, it was not officially adopted as a legal standard until 1980 when New China promulgated its first nationality act. Sole nationality, initiated as an expedient for foreign policy, was originally designed to help with neighbourliness. However, not only did it fail to achieve this goal, but it even resulted in more domestic institutional discrimination among Chinese people. Nowadays, in such a globalization and ‘humanrightization’ era, international law and domestic nationality laws in most countries throughout the world recognize an individual’s right to a nationality, and accept dual nationality so as to facilitate migrants’ returning to their homelands and help them reintegrate into local communities. Contemporary theory and practice of international law support the legitimacy of dual nationality. Also, China has experience in dealing with dual nationality. It would therefore be legitimate, beneficial and practical for China to restore dual nationality.

Infection with Helicobacter pylori leads to gastritis, peptic ulcers and gastric cancer. Moreover, when the gastric mucosa is exposed to H. pylori, gastric mucosal inflammatory cytokine interleukin-8 (IL-8) and reactive oxygen (ROS) species increase. Tribulus terrestris L. is an annual creeping herb of the family Zygophyllaceae. In this study, the effect of Tribulus terrestris L. fruits extract (TTE) extract on H. pylori-infected human gastric epithelial cells was examined. Cell viability was determined by the tetrazolium salt reduction method using WST-1 according to the manufacturer's instructions. AGS cells were pretreated with TTE extract for 24 hrs followed by H. pylori infection for up to 24 hrs. IL-8 secretion in AGS cells was measured by ELISA. The extract yield of the fruits of Tribulus terrestris with 50% ethanol was 20.60%. We analyzed TFE composition by LC. The concentration of Protodioscin in TFE was 310 μg/mL. It was confirmed that exposure to 100 μg/mL of TTE had no significant effect on cell proliferation at the concentrations examined (12.5–200 μg/mL). It was therefore concluded that TTE at these concentrations had no cytotoxic effects on AGS cells and could be used in this study. Pretreatment of H. pylori-infected AGS cells with 12.5, 25, 50 and 100 μg/mL of TTE for 24 hrs significantly decreased IL-8 production by 12.5%, 25%, 27.5% and 50%, respectively, compared to H. pylori-infected cells without TTE. In this study, we found that TTE inhibited H. pylori-induced IL-8 secretion, thus augmenting their benefit in regard to protection of gastric epithelial cells. This study suggests that ingestion of these plant extracts could have therapeutic implications for patients with H. pylori induced gastritis and duodenal ulcer.

We have previously shown that the specific phosphatidylinositol 3-kinase inhibitor LY294002 (LY29), and its inactive analog LY303511 (LY30), inhibit a monocyte chemoattractant protein-1 (MCP-1) expression in human umbilical vein endothelial cells; these results suggest the potential of LY30 as an anti-inflammatory drug. In this study, we determined the effects of LY30 on the production of various inflammatory cytokines in human macrophagic THP-1 cells which were stimulated with lipopolysaccharide (LPS). LY30 selectively suppressed the mRNA expression of IL-12 p40, TNF-α, and MCP-1 without affecting the expression of IL-1α, IL-6, and IL-8. Inhibition of the production of IL-12 and TNF-α by LY30 was also demonstrated using ELISA assays. In order to elucidate the mechanisms of the action of LY30, we examined the role played by the mitogen-activated protein kinases and the key transcription factors, AP-1 and NF-κB in LPS-stimulated THP-1 cells. The results revealed that LY30 inhibited LPS-induced activation of ERK, but not p38 or JNK. Furthermore, the AP-1 DNA binding activity was suppressed by LY30 based upon the dosage, whereas NF-κB DNA binding was not affected. These results suggest that LY30 selectively inhibits cytokine production in the LPS-stimulated macrophagic THP-1 cells by downregulating the activation of ERK and AP-1.

Human malignant melanoma is an aggressive skin cancer which has been rising at a greater rate than any other cancers. Although various new therapeutic methods have been developed in previous studies, this disease has properties of high proliferation and metastasis rate which remain obstacles that have lead to a poor prognosis in patients. It has been reported that a specific Lactobacillus extract has anti-cancer and –metastasis effect in vitro and in vivo. However, previous research has not specified precisely what effect the Lactobacillus rhamnosus GG (LGG) extract has had on human malignant melanomas. In this study, we showed that the LGG extract has anti-cancer and –metastasis effects on the human malignant melanoma cell lines, A375P and A375SM. At first, it was found that, while the LGG extract affects human neonatal dermal fibroblasts slightly, it induced the dose-dependent anti-cancer effect on A375P and A375SM by a WST-1 proliferation assay. As a result of a real-time PCR analysis, the expression patterns of several genes related to cell cycle, proliferation, and apoptosis were modulating in a manner that inhibited the growth of both malignant melanoma cell lines after the treatment of the LGG extract. Furthermore, genes related to the epithelialmesenchymal transition were down-regulated, and migration rates were also decreased significantly by the LGG extract. Our study showed that the LGG extract could be used as a potential therapeutic source.

This study was conducted to Jindo dogsprovide whether Jindo dogs can distinguish cancer cells, early detection. Jindo dogs were trained for cancer detection based on obedience, smell recognition, and tracking. Cancer detection training was designed to enable detection of lung cells (A549), prostate cells (PC 3), or breast cells (MCF7) among 9 pure media. Four Jindo dogs were trained by the same trainer and our different results were obtained (true positive, true negative, false positive, false negative). The accuracy was 85%, 82%, 92%, and 81% for sensitivity and 95%, 89%, 95%, and 93% for specificity. In addition, 4 Jindo dogs can find what is the cancer cell cannot distinguish 3 kinds of (A549, PC3, MCF7). Jindo dogs can detect cancerous cells through human’s breath and urine.

Although anti-aging activities of melatonin, a hormone secreted by the pineal gland, have been reported in senescence-accelerated mouse models and several types of cells, its impact and mechanism on the senescence of human dental pulp cells (HDPCs) remains unknown. In this study, we examined the impact of melatonin on cellular premature senescence of HDPCs. Here, we found that melatonin markedly inhibited senescent characteristics of HDPCs after exposure to hydrogen peroxide (H2O2), including the increase in senescence-associated β-galactosidase (SA-β-gal)-positive HDPCs and the upregulation of p21 protein, an indicator for senescence. In addition, as melatonin attenuated H2O2-stimulated phosphorylation of c-Jun N-terminal kinase (JNK), while selective inhibition of JNK activity with SP600125 significantly attenuated H2O2-induced increase in SA-beta-gal activity. Results reveal that melatonin antagonizes premature senescence of HDPCs via JNK pathway. Thus, melatonin may have therapeutic potential to prevent stress-induced premature senescence, possibly correlated with development of dental pulp diseases, and to maintain oral health across the life span.

Curcumin have various health-beneficial properties in numerous studies. However, its bioavailability is low due to its limited intestinal uptake and rapid metabolism. This study aimed to evaluate the pharmacokinetics of newly developed sub-micron particle curcumin with increased water dispersibility (Theracurmin® CR-033P). Plasma curcumin levels were measured at 0, 1, 2, 4, 8 h after Theracurmin® CR-033P intake using high-performance liquid chromatography. For analyzing pharmacokinetics of Theracurmin® CR-033P, eighteen healthy subjects were recruited and received Theracurmin® CR-033P at a single oral dose containing curcumin 30 mg. Cmax was 28.14 ng/ml, and the area under the curve for 8 h was estimated to be 104.36 ng/ml. Based on the area under the plasma concentration (AUC), the bioavailability of sub-micron particle curcumin was higher 22-, 35-, 28-fold than native curcumin in men, women, and all subjects, respectively. For comparing by formulation, seven healthy subjects were recruited and received two type of treatment: (1) existing dosage form 300 mg (contained curcumin 30 mg) × 3 capsule, (2) high dosage form 300 mg (contained curcumin 90 mg) × 1 capsule + placebo 300 mg × 2 capsule. In the cross-over study, there was no significant differences in Cmax and AUC of plasma curcumin. In conclusion, submicron particle curcumin with increased water dispersibility significantly improved its oral bioavailability and women absorbed curcumin more effectively than men. Different formulation of Theracurmin® CR-033P has shown equivalent to the reference in terms of pharmacokinetics.

목적 : 본 연구는 작업치료전공 대학생을 대상으로 인권감수성 수준을 알아보고 변인 간 인권감수성의 차이 를 비교 분석함으로써 추후 작업치료 전공 대학생의 인권관련 직업 윤리 교과과정 개발 및 인권 행동 제고를 위한 기초자료를 제공하고자 하였다. 연구방법 : 연구대상자는 편의표집에 의해 선정한 전국의 작업치료학과에 재학 중인 작업치료학 전공 대학 생들로 일반적 특성과 인권감수성 에피소드 10개로 구성된 구조화된 설문지를 배포·수거하여 최종 자 료 분석에 408부를 사용하였다. 결과 : 인권감수성은 전체평균 60.60±26.72점 이었으며, 여학생이 남학생보다, 학년이 올라갈수록 인권감 수성이 높음을 알 수 있었다. 인권관련 교육의 경험이 있는 경우 경험이 없는 학생들보다 인권감수성이 높게 나타났으나 통계적으로 유의미하지 않았다. 에피소드 별로는 이주 노동자의 정당한 노동의 권리에 대한 항목의 감수성이 가장 높았고 구금으로부터의 자유권 항목의 감수성이 가장 낮았으며 이는 학년 간 차이가 있었다. 하위요인별 인권감수성 차이의 결과, 모든 학년에서 상황지각이 가장 높게 나타났다. 학년별로는 상황지각은 2학년이, 결과지각과 책임지각은 3학년이 가장 높게 나타났다. 결론 : 작업치료학 전공학생들은 학년이 올라가면서 자신과 타인에게 미칠 결과에 대한 부분을 고려하고 타 인을 인식하는 능력인 결과지각, 인권과 관련된 행동에 책임을 지고 실제 행동으로 실천하고자 하는 책 임지각이 향상되는 것을 알 수 있었다. 본 연구결과를 근거로 교육과정 내 인권의식과 인권 행동이 향상 될 수 있는 내용이 강화되도록 하고, 교육 및 임상에서 작업공정성을 높이기 위한 작업치료실천 관련 논 의가 필요하겠다.

이 글은 지속적인 탈북자문제와 북한인권개선 방안에 관한 것이다. 탈북 자는 1990년대 후반부터 지속적으로 한국행 입국을 해왔다. 북한주민의 탈북배경은 1990년대 중반까지는 북한 자체 ‘고난의 행군시기’의 경제적 동기가 가장 컸다. 하지만 이러한 탈북동기는 2000년대를 거치면서 기획 탈북, 가족단위의 집단탈북, 자신의 미래를 위한 탈북 등의 형태 등으로 바뀌어왔다. 탈북자들은 탈북 후 한국입국 전까지 중국과 제3국에서 불법 체류자 신분으로 인권사각지대에서 엄청난 인권침해를 당하고 있다. 그동안 한국이 탈북자문제에 대해 정부와 민간부문에서 꾸준한 노력을 해 왔 음에도, 탈북자문제와 인권문제에 대해 정부와 민간부문에서 지속적인 공 동의 역할은 더 절실하다. 정부는 2016년 3월 국회에서 북한인권법 통과 로 이 법안에 따라 북한인권문제를 제도적 절차에 따라 탈북문제를 인간의 보편적 가치와 민족문제로 인식하고 국내외적으로도 공동협력해 나가는 다각적인 노력을 기울여야 한다.

Transplantation is considered to be a very useful approach to improve human welfare and to prolong life-span. Heterologous organ transplantation using pig organs which are similar to human beings and easy to make mass-production has known as one of the alternatives. To ensure potential usage of the pig organ for transplantation application, it is essentially required to generate transgenic pig modifying immuno-related genes. Previously, we reported production of heterozygous α 1,3-galactosyltransferase (GalT) knock-out and human membrane cofactor protein (MCP) expressing pig (GalT-MCP/+), which is enforced for suppression of hyperacute and acute immunological rejection. In this study, we reported generation of homozygous pig (GalT-MCP/-MCP) by crossbreeding GalT-MCP/+ pigs. Two female founders gave birth to six of GalT-MCP/-MCP, and seven GalT-MCP/+ pigs. We performed quantitative real-time PCR, western blot, and flow cytometry analyses to confirm GalT and MCP expression. We showed that fibroblasts of the GalT-MCP/-MCP pig do not express GalT and its product Gal antigen, while efficiently express MCP. We also showed no expression of GalT, otherwise expression of MCP at heart, kidney, liver and pancreas of transgenic pig. Taken together, we suggest that the GalT-MCP/-MCP pig is a useful candidate to apply xenotransplantation study.

After being subjected to different cooking methods, small black beans (Rhynchosia nulubilis) were investigated in order to assess the effects of the retained bioactive compounds. Using uncooked, pan broiled, boiled, steamed, and pressure cooked beans, the inhibitory effects of MCF-7 cell migration were evaluated at protein concentrations of 40, 160, and 640 μm/mL, using the Boyden's chamber assay. All protein concentrations (40, 160, and 640 μm/mL) of pan broiled beans showed significant reduction (59.83, 32.48, and 21.37%, respectively) in the rate of cell migration to the lower chambers (p-value less than 0.001). Estimated cell migration rates correlated to the exponential decay between experimentally measured cell migration rates and converted samples. The range of estimated cell migration rate for each 100 mg/mL of cooked sample was as follows: pan broiled (21.16%), boiled (22.48%), steamed (22.48%), pressure cooked (29.52%), and uncooked (35.03%) beans. Our study indicated that selective modifications of cooking methods for small black beans, such as pan broiling, ameliorated the inhibitory effects of MCF-7 cell migration. This suggests that optimized cooking methods increase the nutritional contents of the cooked food.

In the present study, sled analysis was conducted under the condition of 20g (20 times of gravitational acceleration) and 48 km/h of initial velocity, suggested by WC-19 to investigate the injuries of traffic minorities of tie-down type fixing inside a vehicle during crash. Also, four new parameters, which were related to angles between belts and wheelchair and their levels were limited by WC-19, were proposed and orthogonal array was prepared with different levels of each parameter. In the meantime, four parameters were optimised by minimising head injury criteria and chest acceleration of passengers in each case of the orthogonal array.

본 연구에서는 산림치유인자인 경관과 청각요소를 활용한 산림의 간접적 영향이 기분개선에 미치는 영향을 알아보고 자 하였다. 실험은 신체건강한 대학생 30명(23.6±1.7세)을 대상으로, 2016년 10월 5일부터 11월 30일 까지 약 2달간 실시되었다. 산림과 도시공간의 경관과 소리를 이용하여 3가지 요소(이미지 요소, 소리 요소 그리고 이미지+소리 요소)를 만들었으며, 대상자들이 실내에서 이 3가지 요소들을 감상하였다. 그리고 3가지 요소들이 기분개선에 미치는 영향들을 SPSS 18.0프로그램을 이용하여 비교분석하였다. 기분개선을 측정하기 위한 심리검사로써 기분상태검사 (POMS)와 의미분별법(SD)이 사용되었다. 그 결과 간접적인 산림의 자극은 긴장, 피로, 분노, 혼란, 우울감을 억제하고 활력감을 증진시키는 효과를 나타내었고 산림의 자극 간 비교에서는 차이를 보지 않았으나 도시의 자극에 비해 산림의 자극이 쾌적감과 자연감 그리고 진정감을 높이는 효과를 가지는 것으로 나타났다.

본 논문은 『히브리 선율』 집에 들어있는 바이런의 초기시가 영혼과 하느님 의 불멸성에 대해 어떠한 종교적인 고찰을 보여주고 있는가를 다룬다. 종교시는 1810년대에 출간되어 시인의 이전 작품 『한가한 시간들』에서 보이는 자연의 항구성을 전개시켜 인간의 영혼과 전능하신 신의 영역까지 다루고 있다. 바이런 은 『히브리 선율』 중 다섯 편의 시를 통해서 인간 영혼의 불가침성을 암시해주 고 있으며 이는 그의 후기시와 여러 수상록에서도 이 부분에 대한 시인의 확고한 믿음을 보여주고 있다. 이러한 바이런의 초기에 나타난 종교적 명상의 씨앗 들은 시인을 1813-1814년에 지은 작품과 1823-1824년에 사이에 지은 『천지』라 는 작품을 토대로 성상파괴자라고 혹평했던 당대 비평가들의 태도가 왜곡되었 음을 여실히 보여주고 있다. 본 논문은 『히브리 선율』이라는 작품을 통하여 이 러한 당대의 비평시류에 반대되는 다양한 예증들을 통하여 바이런이 이들과 상 반되는 종교적 시각을 드러냄을 보여주고자 한다.

BMP-2 is a well-known TGF-beta related growth factor, having a significant role in bone and cartilage formation. It has been employed to promote bone formation in some clinical trials, and to differentiate mesenchymal stem cells into osteoblasts. However, it is difficult to obtain this protein in its soluble and active form. hBMP-2 is expressed as an inclusion body in the bacterial system. To continuously supply hBMP-2 for research, we optimized the refolding of recombinant hBMP-2 expressed in E. coli, and established an efficient method by using detergent and alkali. Using a heparin column, the recombinant hBMP-2 was purified with the correct refolding. Although combinatorial refolding remarkably enhanced the solubility of the inclusion body, a higher yield of active dimer form of hBMP-2 was obtained from one-step refolding with detergent. The refolded recombinant hBMP-2 induced alkaline phosphatase activity in mouse myoblasts, at ED50 of 300-480ng/ml. Furthermore, the expressions of osteogenic markers were upregulated in hPDLSCs and hDPSCs. Therefore, using the process described in this study, the refolded hBMP-2 might be cost-effectively useful for various differentiation experiments in a laboratory.

Treatment of dextran sodium sulfate(DSS) on HeLa cells results to an enhanced susceptibility to Brucella(B.) abortus infection. An increase in the adherence, invasion and intracellular replication of B. abortus was observed in DSS-treated cells. Furthermore, a marked elevation in the intensity of F-actin fluorescence was also observed in DSS-treated cells compared with untreated B. abortus-infected cells. An upregulation of phagocytic signaling proteins by Western blot analysis demonstrated an apparent activation of ERK, p38α and JNK phosphorylation levels in B. abortus-infected DSS-treated cells compared with the control. Colocalization with LAMP-1 proteins was attenuated in DSS-treated cells upon intracellular trafficking of the pathogen compared with control cells. The results of this study demonstrated consistency with other pathogens. The uptake and intracellular replication of B. abortus is hypothesized to be stimulated by various dextran receptors such as C-type lectins that are involved in phagocytosis which can either be direct phagocytic receptors, modulators of the expression of other receptors or as opsonins leading to an enhanced internalization of B. abortus. The complexity of these interactions thus would warrant further investigation into the role of DSS in the pathogenesis of brucellosis. In summary, we conclude that DSS enhanced adhesion, phagocytosis and intracellular replication of B. abortus in epithelial cells which could lead to suppression of the innate immune system in chronic Brucella infection.

Colorectal cancer is a major cause of morbidity and mortality that accounts for over 9% of all incidences of cancer. Additionally, colorectal cancer is widely recognized as an environmental disease related to ill-defined cultural, social and lifestyle factors including physical activity, obesity, cigarette smoking and heavy alcohol consumption. Accordingly, natural phytochemicals and extracts have attracted attention because of their beneficial biological effects. Coenzyme Q10 (CoQ10) is a common supplementary medicine applied to increase bioenergetic capacity in various diseases. Therefore, in this study, we investigated whether CoQ10 treatment has any inhibitory effects and its related cellular mechanisms in human colon cancer HCT116 cells. A MTT assay revealed that CoQ10 slightly decreased the proliferation of HCT116 cells; however, glutathione- and superoxide dismutase- activity were unchanged in response to CoQ10 treatment. A DCF-DA assay revealed that CoQ10 slightly increased ROS release of HCT116 cells. However, in a nitric oxide (NO) assay, CoQ10 significantly increased NO production in a dose-dependent manner. The results of western blot analysis revealed that the protein levels of Bax, p21 and p53 were increased, whereas the protein level of Bcl2 was decreased suggesting that the CoQ10-mediated inhibitory mechanism is associated with apoptotic signaling. Taken together, our findings indicate that CoQ10 has an inhibitory effect on the growth of colon cancer cells via NO production that is associated with regulation of factors involved in apoptotic signaling including Bax, Bcl2, p21 and p53.