한국에서 말라리아는 1993년에 다시 등장했으며, 이후 증감을 반복하며 공간적 변화를 거듭하여 왔다. 지리 공간의 인문·자연·지리적 요인들은 복잡하게 상호작용하여 매개 모기의 생태와 말라리아 발생에 영향을 미친다. 본 연구는 말라리아의 발생 양상을 이해하고 이를 조절하는 데 기여하고자 그 공간적 패턴을 분석하였다. 본 연구는 Getis-Ord의 Gi* 와 평균 중심점을 이용하여 2001년부터 2014년 사이 말라리아 발생의 공간적 분포를 탐색적으로 분석하여, 그 시공간적 변화를 파악하였다. 또한 일반화 포아송 회귀모형을 이용하여 2001년, 2014년 말라리아에 영향을 미치는 인문·환경 요인을 비교·분석하고, 군사분계선으로부터의 거리 변수를 이용하여 그 공간적 자기상관을 설명하고자 하였다. 그 결과, 소 두수, 표준공시지가, 성비, 아파트 비율, 군사분계선 거리, 기후 요소, 논 비율이 말라리아 발생에 영향을 미치는 유의한 변수로 확인되었다.

Although stem cells are used as important cell therapies in regenerative medicine, the electrophysiological problems that arise in the expansion of cells have not been known much. This study was conducted to investigate the functional expression of inward rectifying K+ current (IKir) using a patch-clamp technique, and the change in the resting membrane potential and the membrane capacitance were investigated in mesenchymal stem cells derived from human umbilical vein (hUC-MSC). The IKir plays an important role in regulating the resting membrane potential in many cells and is known to contribute to the maintenance of intracellular K+ concentration. In this study, electrophysiologically recorded current exhibited typical IKir characteristics. The current shifted along the K+ equilibrium potential (Ek) with the extracellular K+ concentration change. In addition, IKir was blocked by the divalent Ba2+ in a dose-dependent manner. The frequency of functional expression of IKir changed with number of passages (P2: 5.3% vs P8: 77.8% vs P12: 34.5%). There was no significant change in the resting membrane potential of hUC-MSC (P2: -21.0 mV, P8: -20.1 mV and P12: -21.9 mV). However, the capacitance of the cell membrane was significantly changed after P9 (P2: 8.9 pF vs P9: 16.9 pF) compared to P2. All the results suggest that changes in electrophysiological distribution of IKir as the passages increase may cause changes in K+ permeability even in cell proliferation and differentiation, suggesting a possible physiological role in maintaining cell homeostasis and resting membrane potential (RMP).

For several years, keyboard and mouse have been used as the main interacting devices between users and computer games, but they are becoming outdated. Gesture-based human-computer interaction systems are becoming more popular owing to the emergence of virtual reality and augmented reality technologies. Therefore research on these systems has attracted a significant attention. The researches focus on designing the interactive interfaces between users and computers. Human-computer interaction is an important factor in computer games because it affects not only the experience of the users, but also the design of the entire game. In this research, we develop an particle filter-based face tracking method using color distributions as features, for the purpose of applying to gesture-based human-computer interaction systems for computer games. The experimental results proved the efficiency of particle filter and color features in face tracking, showing its potential in designing human-computer interactive games.

In the construction sites, rush (speedy construction) work have recently been overused because of budget reductions by reducing the construction duration after the long-term economic recession and construction business stagnation with institutional change including the instruction of the duration shortening bidding system and the introduction of the post-construction sales system by the Korea Research Institute for Human Settlements. Due to this, negligent accidents have frequently occurred by forcing manpower, equipment, finance, and material procurement and pressing ahead with impractical construction wok at night according to the reduction of construction schedules. Therefore, this paper has the necessity of this study to seek for alternatives to prevent negligent accidents by finding factors related to rush work in the reality that the importance for preventing negligent accidents is more emphasized while they have not been reduced in construction sites.

하이테크놀로지를 여러 가지 다른 영역과 융합하고자 하는 노력이 다양한 연구 분야에서 시도되고 있으며, 스포츠웨 어를 개발함에 있어 운동선수의 운동능력을 향상시키기 위한 분야에 이러한 첨단 기술들이 도입되고 있다. 본 연구는 스포츠 훈련에 도움이 되는 동작 센싱 스마트 스포츠웨어를 개발하기 위한 기초 연구로서, 조정 동작 시 관절의 움직임 을 측정할 수 있는 동작 센서를 부착하기 위한 인체상의 최적 위치를 탐색하는 것을 목적으로 한다. 본 연구에서는 일관된 동작을 반복적으로 수행할때 관절의 변화가 큰 조정을 대상 스포츠로 선정하였으며, 조정 선수의 대표 체급인 중량급과 경량급의 피험자를 선정하여 동작에 따른 피부의 변화율을 측정하여 체급별 차이를 분석하였다. 먼저, 3차원 모션캡처 시스템을 이용하여 조정 동작 시 등, 팔꿈치, 엉덩이, 무릎 부위의 피부 변화를 촬영하고, 각 마커 간 거리의 변화율을 분석함으로써 체급에 따른 차이를 보이지 않으면서 동작에 따라 피부의 변화가 큰 부분을 인체 상에 도시하였 다. 이를 바탕으로 동작 센싱용 스마트 스포츠웨어를 위한 센서 부착의 가이드라인을 제시하였다.

Recently, the area of marine resources has become concerned with sources for the next generation of the bio-industry. Until present, development of the marine resources has remained limited, although a large number of these resources are considered to have potential for various significant biological activities. Most marine sponges, marine algae and coral could be used to create specific compounds for survival against a harsh environment. Therefore, it was necessary that these materials needed to be elucidated with biological activities, such as like anti-inflammatory, anti-viral or anti-cancer effects for their utilization in the bio-industry. In this study, we screened extracts of marine resources for their anti-cancer effect on human colorectal cancer cells. These resources were collected at Kosrae of Micronesia on April, 2013 and extracted with methanol. Cytotoxicity of marine resources was observed. Of a total of 20 specimens, three specimens dose-dependently demonstration inhibition of cell viability. Furthermore, cells treated with these specimens for 48h were induced p53, p21, Bax and caspase-3. The results suggest that they involved p53-mediated apoptosis. Two positive specimens (1304KO-327 and 1304KO-329) were verified as the identical materials, which are Hyrtios sp. Unfortunately 1304KO-207 was not yet classified and needed to identify in the further study. There results suggested that marine resources with positive potential in anticancer effect would be good candidates as useful bio-resources.

Lycorine, a natural alkaloid extracted from the Amaryllidaceae plant family, was reported to various physiological and pharmacological effects including anti-cancer activity. Nevertheless, there is no report of the anticancer effect of lycorine in oral cancer cells. The effects of lycorine on cell proliferation and apoptosis were examined through trypan blue exclusion assay, 4’-6-diamidino-2-phenylindole (DAPI) stain, Live/Dead assay, Western blot analysis and RT-PCR. Lycorine suppressed cell viability and induced apoptosis in MC3 and HSC-3 cell lines. Lycorine decreased survivin protein but did not affect its mRNA. It regulated survivin through accelerating protein degradation in a time-dependent manner although neither proteasome nor lysosome was not associated with lycorine-mediated protein degradation. Collectively, our results suggest that lycorine may be a potential therapeutic anti-cancer drug candidate for the treatment of human oral cancer.

Anthricin (Deoxypodophyllotoxin), a naturally occurring flavolignan, has well known anti-cancer properties in several cancer cells, such as prostate cancer, cervical carcinoma and pancreatic cancer. However, the effects of Anthricin are currently unknown in oral cancer. We examined the anticancer effect and mechanism of action of Anthricin in human FaDu hypopharyngeal squamous carcinoma cells. Our data showed that Anthricin inhibits cell viability in a dose- and time-dependent manner (IC50 50 nM) in the MTT assay and Live & Dead assay. In addition, Anthricin treated FaDu cells showed marked apoptosis by DAPI stain and FACS. Furthermore, Anthricin activates anti-apoptotic factors such as caspase-3, -9 and poly (ADP-ribose) polymerase (PARP), suggesting that caspase-mediated pathways are involved in Anthricin- induced apoptosis. Anthricin treatment also leads to accumulation of the pro-apoptotic factor Bax, followed by inhibition of cell growth. Taken together, these results indicate that Anthricn-induced cell death of human FaDu hypopharyngeal squamous carcinoma cells is mediated by mitochondrial-dependent apoptotic pathway. In summary, our findings provide a framework for further exploration on Anthricin as a novel chemotherapeutic drug for human oral cancer.

Tyrosol, a phenylethanoid and a derivative of phenethyl alcohol, possesses various biological properties, such as anti-oxidative and cardioprotective activity. Olive oil is the principal source of tyrosol in the human diet. However, so far the anti-cancer activity of tyrosol has not yet been well defined. This study therefore undertakes to examine the cytotoxic activity and the mechanism of cell death exhibited by tyrosol in KB human oral cancer cells. Treatment of KB cells with tyrosol induced the cell growth inhibition in a concentration- and a time-dependent manner. Furthermore, the treatment of tyrosol induced nuclear condensation and fragmentation of KB cells. Tyrosol also promoted proteolytic cleavage of procaspase-3, -7, -8 and –9, increasing the amounts of cleaved caspase-3, -7, -8 and –9. In addition, tyrosol increased the levels of cleaved PARP in KB cells. These results suggest that tyrosol induces the suppression of cell growth and cell apoptosis in KB human oral cancer cells, and is therefore a potential candidate for anti-cancer drug discovery.

The objective of this study was to investigate the effects of a lettuce (Lactuca sativa L.) extract on the inflammation of human umbilical vein endothelial cell (HUVEC) and blood lipid improvement in hypercholesterolemic mice fed a high cholesterol diet. The lettuce extract (100% ethanol extract) inhibited the expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 in HUVEC treated with tumor necrosis factor-α (TNF-α). The lettuce extract suppressed the adhesion of THP-1 to TNF-α-treated HUVEC. The lettuce extract decreased the TNF-α-stimulated production of proinflammatory cytokine interleukin-6, interleukin-8 and chemokine monocyte chemotactic protein 1. In hypercholesterolemic mice, the lettuce extract reduced serum total cholesterol, triglyceride, and low-density lipoprotein-cholesterol level, while the lettuce extract elevated high-density lipoprotein-cholesterol level, resulting in the decrease of atherogenic index and cardiac risk factor level. These results suggested that lettuce extract can be an useful resource to show an anti-inflammatory effect and improve lipid metabolism

NNK (4-(methylnitrosamino)―1-(3-pyridyl)-1-butanone) is a major form of nitrosamine abundant in cigarette smoke and is a powerful carcinogen. Mercury is a major component of the amalgam that is widely used as dental filling material. Concurrent exposure to these two agents may result in their interaction and alter their carcinogenic potential. The present study used an immortalized human epithelial cell system that allows continuous exposure to potential carcinogens, in an attempt to elaborate the carcinogenic potential of mercury and NNK in humans. Cytotoxicity of mercury chloride and NNK was measured by an MTT assay. Parameters of neoplastic cellular transformation such as cell saturation density, soft-agar colony formation, and cell aggregation were analyzed to examine the carcinogenic potential of mercury chloride and NNK. The study showed that exposure to mercury chloride with NNK resulted in increased soft agar colony formation and cell aggregation. ROS generation by mercury chloride was further enhanced by treatment with NNK. The apoptosis that was observed following mercury chloride exposure was further increased upon co-treatment with NNK. The interaction between these two agents was also observed in cytokine mRNA induction. In the present study, mercury alone did not seem to pose a significant threat as a carcinogen, but it may have potential to enhance the carcinogenic potential of a known carcinogen from cigarette smoke. The present study provides valuable data regarding the evaluation of potential carcinogenic risk of mercury chloride and NNK on concurrent exposure.

본 연구의 주제는 포스트휴먼 시대 사이보그의 알레고리이다. 아르 오리앙테 오브제의 퍼포먼스, <아 마도 내 안에 말이 살고 있을지도 몰라>(2011)에서 마리옹 라벨 장테는 인간과 동물, 기계가 혼합한 하이 브리드 유기체가 되었다. 인간에서 ‘켄타우로스 사이보그’ 변신은 우리에게 인간과 비인간 사이 경계의 재배열과 재조정이 나타나는 포스트휴먼 시대의 새로운 지형학을 바라 볼 수 있는 기회를 제공 해 준다. 본 연구자의 화두는 그녀의 변신 과정에서 동물과 기계가 결합한 타자되기가 무엇을 의미하며, 어떻게 인간이 포스트휴먼 주체가 되는가이다. 이를 살펴봄으로써 이 논문은 아르 오리앙테 오브제의 사이보그 가 동시대 미술에 나타나는 새로운 신체인 하이브리드 유기체로서 뿐만 아니라 포스트휴먼 주체 생성과 정의 알레고리로서 작동하고 있음을 밝히고자 한다.

A recent study reported that Pleurotus ostreatus has the potential to be used as a β-glucan-based cream for supportive complementary therapy of atopic dermatitis. KH054 is a new herbal prescription consisting of P. ostreatus and Panax ginseng. The effects of atopic dermatitis-induced materials on the expression of cytokine genes in human monocytes (THP-1, EoL- 1) have been examined. Some reports demonstrated that P. ginseng augments the activity of natural killer cells, which plays an important role in innate immunity against infection and tumor development. Monocyte chemotactic protein 1 (MCP-1), interleukin (IL)-6, and IL-8 have important roles in mediating the infiltration of various cells into the skin of atopic dermatitis and psoriasis. The present study investigated whether KH054 on induced IL-6, IL-8, and MCP-1 secretion by house dust mite (Dermatophagoides pteronissinus) in THP-1 (human acute monocytic leukemia) and EoL-1(Human eosinophilic leukemia) cell. D. pteronissinus functions in the pathogenesis of allergic diseases, including atopic dermatitis and asthma. The inhibitory effect of KH054 on the induction of IL-6, IL-8, and MCP-1 secretion by D. pteronissinus extract in THP-1 and EoL-1 cells was examined. KH054 potently suppressed the elevated production of IL-6 and IL-8 induced by D. pteronissinus treatment in THP-1 and EoL-1 cells. Based on the present results, KH054 may be useful for developing functional foods to treat atopic dermatitis.

선박의 기계식 환기시스템은 화재발생 시 연기의 생성과 확산 특성에 영향을 미치고, 이는 피난자의 피난경로 상의 가시도를 방해함으로써 피난자의 연기로 인한 피해를 증가시킬 위험성이 크다. 이에 이 연구에서는 선박 거주구역에서 화재발생 시 기계식 급·배기시스템이 연기확산에 미치는 영향과 위험성에 대하여 FDS를 활용하여 평가하고 화재 시 급·배기시스템을 효율적으로 사용할 수 있는 방안을 제안하였다. 연구결과 화재가 발생한 장소에 급·배기시스템이 함께 작동되고 있는 경우에는 현재 권장되고 있는 급·배기시스템을 멈추는 방법보다 작동을 유지하는 것이 효과적이고, 배기시스템만 작동되는 곳에서 화재가 발생한 경우에는 화재가 발생한 구역 이외의 구역에서 급기시스템을 함께 작동시키는 것이 피난시간을 확보하는데 효과적인 것으로 예측되었다. 그러나 화재가 발생한 곳에 급기시스템만 있는 경우에는 급기시스템이 연기확산을 가속시키기 때문에 급기방식을 중단시켜 연기의 확산을 최대한 억제할 필요가 있음을 확인하였다.

In March 2014 the Myanmar Hluttaw, or Parliament, enacted the Myanmar National Human Rights Commission Law, which provided a statutory basis for a national human rights body in Myanmar. The Myanmar government declared to the United Nations Human Rights Council that the Enabling Law was compliant with the United Nations Paris Principles that set international standards for national human rights institutions. Despite the claims of the Myanmar government, however, critics charge the Enabling Law is insufficient, with detractors claiming the law leaves the MNHRC with anaemic powers incapable of advancing human rights. This paper responds to such issues by conducting an independent evaluation of the MNHRC Enabling Law under the Paris Principles. In doing so, the analysis treats the Enabling Law as a case study demonstrating how the Paris Principles can be exercised by third parties as the UN-supported international standards for national human rights institutions.

Human interferon alpha 2b (hIFNα-2b) is an important immune regulator widely used in clinic, for the treatment of chronic hepatitis, hairy cell leukemia, chronic myelogenous leukemia and multiple myeloma, etc. The clinically used hIFNα-2b is generally produced by E. Coli, which lacks the post-translational O-glycosylation of naturally synthesized protein, and has a short serum half-life. In this study, we report the successful generation of transgenic chickens that produce hIFNα-2b in the egg white using a feline immunodeficiency virus (FIV)-based lentiviral vector. In preliminary in vitro study, the hIFNα-2b gene under the control of CMV promoter expressed as much as 2,650 ng/㎖ in CEF-LNC-hIFNα-2bW cell. A FIV vector packaged with vesicular stomatitis virus G glycoprotein (VSV-G) was injected underneath the blastoderm of freshly laid chicken eggs (stage X) to produce a hIFNα -2b transgenic chicken. Out of 187 injected eggs, 55 chicks were hatched after 21 days of incubation, and 27 of the G0 hatched chicks expressed the vector-encoded hIFNα-2b gene. The expression of recombinant hIFNα-2b in transgenic chickens constitutes an important step towards low-cost and full biological activity production of this protein drug in bioreactor. This work was supported by the Bio-industry Technology Development Program, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea, and by a grant from the Next-Generation BioGreen 21 Program (No. PJ011178), Rural Development Administration, Republic of Korea.