This study was carried out to evaluate the microbiological characteristics of retail chicken meats stored under various conditions. Nine of whole chickens and nine of chicken breasts were used for bacterial analysis. Each chicken meat was divided into subsamples of 25 g each followed by storage at room temperature (25℃), refrigeration temperature (4 ℃), and freezing temperature (—20℃) for 180 min, 5 days, and 3 days, respectively. The standard plate counts were performed for the enumeration of the total aerobic bacteria. The number of aerobic bacteria was gradually increased by 1 log in samples held at the room temperature for 180 min. There was statistical difference in the number of bacteria between at 0 min and at 180 min of storage. For samples stored at 4℃ for 5 days, the number of bacteria was increased from 5.11 to 7.26 log CFU/g in chicken breast and 3.83 to 6.04 log CFU/g in whole chicken with statistical difference. No significant changes were observed in frozen chicken. The results of this study may provide useful information to consumers for proper storage and safe handling of chicken meats.

In this study, we estimated nitrogen dioxide (NO₂) concentrations in microenvironments where residential indoor, residential outdoor, other indoors, and transportation using measured personal exposure and multiple linear regression analysis of time-weighted average model, and compared with measured NO₂ concentration in microenvironments. Measured residential indoor, outdoor and other indoor NO₂ concentration was 22.22±9.59 ppb, 23.64±9.62 ppb, and 22.07±13.90 ppb, respectively. NO₂ concentrations in residential indoor and outdoor, total outdoor, other indoor, and transportation by multiple regression analysis were significantly estimated as 20.48 ppb, 32.79 ppb, 24.35 ppb, and 28.82 ppb, respectively (p= 0.000). Measured and estimated NO₂ concentration were similar with each other, therefore NO₂ concentrations in each microenvironment were able to be estimated using time-weighted average model and personal exposure with multiple regression analysis.

본 시험은 이탈리안 라이그라스 극조생종 품종을 육성하기 위하여 2001년부터 2010년까지 국립축산과학원 초지사료과에서 수행되었다. 이탈리안 라이그라스 신품종 '그린팜'는 2배체 품종으로서 엽은 녹색이며, 월동 전 초형은 직립형이고, 봄의 초형은 반직립형이다. 또한 '그린팜'는 4월 28일경에 출수하는 극조생종 품종으로 '그린팜'은 'Florida 80' 보다 지엽폭이 0.4mm 넓고, 지엽의 길이가 0.8 cm 길었으며, 출수기에 초장은 93 cm로

Biodegradable octopus pot was developed to reduce plastic pollution problem in the sea and fishing trouble between fishermen. It can be expect to recycle other wasted biodegrade fishing gear. Experimental fishing was carried out to understand the difference in fishing efficiency between Polyethylene (PE) octopus pots and biodegradable (Polybutylene Succinate and Polybutylene adipate-co-terephthalate) octopus pots which was tried to make in this study in the sea. There were caught by 237 numbers of fishing during the experimental period. Among the 237 numbers of fishing, 160 or 67.5% were PE pots which were more than the biodegradable pots. A comparison of the monthly catches between the PE pots and biodegradable pots shows that the catches were overall higher in the PE pots than in the other pots. The result is very similar with the comparison of total catches by each type of the pots. In terms of bycatch, the number of species, amount of catches and the number of fishing with bycatch were more significant in the biodegradable pots than in the PE pots.

In order to describe the performance of a wedge type jellyfish excluder device, a series of fishing experiments was carried out in the coastal areas of Yokji Island, southern Korea in 2009, using a trawl net with a cover net. The body size and weight of each individual (fish or jellyfish) caught in the experimental fishing were measured. In the case of giant jellyfish the bell diameter and weight were measured. The catch species was composed of giant jellyfish (Nemopilema nomurai), silver croaker (Pennahia argentata), yellow croaker (Larimichthys polyactics), finespotted flounder (Pleuronichthys cornutus), largehead hairtail (Trichiuruslepturus), melon seed (Psenopsisanomala) and so on. The weight ratio and individual ratio of total fish escaped through the outlet of the excluder device were 0.322 and 0.320, respectively. The weight ratios of giant jellyfish excluded from the trawl net ranged from 0.740 to 0.921 (average 0.852/haul). It means that the wedge type jellyfish excluder device performed well and allowed the most of the giant jellyfish to exclude through the trawl net. The approximately 70% of fish entered in trawl net was caught. The wedge type excluder device needs some improvements to minimize the fish escape from the trawl nets in the future.

시판되는 친환경유기농자재 19종을 대상으로 실내에서 퉁퉁마디뿔나방 (Scrobipalpa salinella) 유충을 대상으로 살충효과를 조사한 결과 90% 이상의 살 충효과를 나타낸 것은 수도응삼이 등 15개 제품이었다. 실내에서 살충효과가 우수 한 수도응삼이 등 5종의 친환경유기농자재 및 자체 개발한 B.t HARI042균주를 대 상으로 전남 신안군 증도면의 함초 재배포장에서 퉁퉁마디뿔나방의 방제효과를 조사하였다. 자체 개발균주인 B.t HARI042균주의 방제효과가 88.1%로 가장 높 았고, 친환경유기농자재인 수도응삼이, 선초 및 멸다이의 순으로 80% 이상의 방 제효과를 보였다. 함초 재배지에서 퉁퉁마디뿔나방의 친환경적인 관리체계는 유 충이 1년에 3회 피해를 주기 때문에 6월 상순부터 8월까지 주 발생시기 초기에 각 각 1회씩 3회 처리하면 퉁퉁마디뿔나방을 안전하고 효과적으로 방제할 수 있었다.

함초 자연발생지(전북 김제시 광활면) 및 재배포장지(전남 신안군 증도면)에서 의 퉁퉁마디뿔나방의 발생생태를 조사한 결과, 2지역 모두 1년에 4회 발생하는 것 으로 조사되었다. 부화유충은 김제지역에서는 5월 중순, 신안지역에서는 5월 하순 부터 발생하기 시작하여 8월 중순까지 발생하였다. 각 세대별 최고 발생시기는 1 세대는 6월 중순, 2세대는 7월 상순, 3세대는 8월 중순 이었다. 그러나 4세대 유충 은 9월 상·중순부터 발생되기 시작하지만 함초에 큰 피해를 주지 못하였다. 퉁퉁마 디뿔나방 성충의 산란은 함초의 아래쪽 목질화된 부위뿐만 아니라 함초 재배지 주 변의 말라 죽은 잔재물에 여러 개의 알을 집중적으로 낳는 특성을 지니고 있다. 따 라서 퉁퉁마디뿔나방은 포장 주변 염생식물에서 월동을 한 후 성충이 포장에 잔존 하는 염생식물에 산란하기 때문에 영농초기에 염생식물의 마른 잔재물 제거를 통 해 이 해충의 피해를 예방할 수 있다.

This study was performed to comprehend the plasma proteins expressed specifically during early pregnancy in pregnant or non-pregnant Hanwoo using proteomic analysis technique. Plasma samples (0, 2, 3, 4, 7, and 11 weeks after AI) were obtained from pregnant (P, n=3) or non-pregnant (NP, n=4) Hanwoo, respectively. To evaluate proteins differentially expressed, 2-dimensional electrophoresis (2DE) was conducted. Normalized protein spots were selected for the significant expression variation deviated over two fold in its expression level between two groups. Molecular functions of the proteins were DNA binding, protein binding, hemoglobin binding, ferrochelatase and transporter activity and arylestera, respectively. According to western blotting, haptoglobin was specifically expressed only in NP group during early pregnancy; however, paraoxonase 1 was highly expressed in pregnant group. Based on these results, pregnancy was maintained successfully by the activation of specific plasma proteins associated with immune system and antioxidant regulation during early pregnancy in Hanwoo

Porcine circovirus type 2 (PCV2) is associated with porcine circovirus diseases (PCVD), of which postweaning multisystemic wasting syndrome is considered to cause considerable economic losses in pig industry worldwide. As the virus-like particle (VLP) is a highly effective type of subunit vaccine and has unique advantages in terms of safety and immunogenicity, this study aimed to develop PCV2-like particles, which matched currently circulating Korean PCV2 and were applicable as vaccines. The ORF2 genes encoding PCV2 capsid protein were amplified from the PCV2 subgroup 1A/B Korean C275 isolate and the subgroup 2E C94 isolate by PCR assay with primer pair specific to PCV2 ORF2 gene, and were cloned into baculovirus transfer vector. Recombinant baculovirus was generated by cotransfection with the transfer vector and linear baculovirus DNA into the Sf9 cells, and then by plaque purification. Expression of PCV2 capsid protein was determined by the indirect immunofluorescence and Western blotting assays, and electron microscopy. By both immunological assays, PCV2 capsid antigen was detected in the Sf9 cells infected with the recombinant baculoviruses. The formation of empty virus particles, characteristic of VLP, was detected in the lysate of Sf9 cells infected with the recombinant baculoviruses by negative electron microscopy. From these results, VLPs of two genogroups of PCV2 were successfully expressed and generated in a baculovirus expression system. It is expected that the expressed VLPs of two genotypic groups can be used for control of PCV2 infection as good vaccine candidates.

In the environment of 450mm wafers production known as the next-generation semiconductor production process, one of the most significant features is the full automation over the whole manufacturing processes involved. The full automation system for 450mm

본 연구는 제주조릿대의 성장단계별 영양소 함량을 분석하고 말에서의 소화율을 검정하여 말 사료자원으로써의 활용가치를 평가하고, 제 주조릿대 군락지 내 말 방목에 따른 제주조릿대의 생산성, 생육특성, 주변 식생의 변화 등을 구명하기 위해 수행되었다. 제주조릿대를 구초 (1년생)와 신초 (당년생)의 성장단계로 분류하여 조단백질, NDF, ADF 및 무기물 함량을 분석했다. 제주마 5두를 이용 제주조릿대 구초와 신초의 전분채취법을 이용한 외관상 소화율을 검정했

Oxide semiconductors Thin-film transistors are an exemplified one owing to its excellent ambient stability and optical transparency. In particular zinc oxide (ZnO) has been reported because It has stability in air, a high electron mobility, transparency and low light sensitivity, compared to any other materials. For this reasons, ZnO TFTs have been studied actively. Furthermore, we expected that would be satisfy the demands of flexible display in new generation. In order to do that, ZnO TFTs must be fabricated that flexible substrate can sustain operating temperature. So, In this paper we have studied low-temperature process of zinc oxide(ZnO) thin-film transistors (TFTs) based on silicon nitride (SiNx)/cross-linked poly-vinylphenol (C-PVP) as gate dielectric. TFTs based on oxide fabricated by Low-temperature process were similar to electrical characteristics in comparison to conventional TFTs. These results were in comparison to device with SiNx/low-temperature C-PVP or SiNx/conventional C-PVP. The ZnO TFTs fabricated by low-temperature process exhibited a field-effect mobility of 0.205 cm2/Vs, a thresholdvoltage of 13.56 V and an on/off ratio of 5.73×106. As a result, We applied experimental for flexible PET substrate and showed that can be used to ZnO TFTs for flexible application.

본 시험은 2007년부터 2008년까지 톱밥발효우분퇴비 시용수준에 따른 오차드그라스의 생산성과 질산염축적에 미치는 영향을 구명하기 위해 난지축산시험장 조사료포장에서 수행되었다. 시험처리는 화학비료 (CF) 200 kg/ha를 대조구로하여 톱밥발효우분퇴비 (CM)를 질소기준 50, 100, 200%를 두어 처리하였다. 건물수량에서는 2년 평균 CM 200%구가 20,177 kg/ha로 CF구 19,165 kg/ha와 비슷한 수량을 보였다. 처리별 평균 조

최근에 폭설로 인한 온실의 붕괴가 빈번해져서 농가의 피해가 증가되고 있다. 히지만 폭설로 인한 온실의 붕괴를 막기 위한 정확한 구조해석에 대한 연구가 미약하여 매년 농가의 피해는 되풀이 되고 있다. 기존 온실의 구조해석 방법은 미소변위에 기초한 선형탄성해석으로 이루어지고 있다. 그러나 실제 온실의 강성은 건축 구조물의 강성에 비해 상당히 약하지만 하중은 폭설에 의해 상대적으로 크게 작용하여 변형이 크게 발생하고 구조물의 기하학적 형태가 변하므로 변형률-변위 관계가 비선형 거동을 한다. 본 연구에서는 폭설에 따른 온실의 붕괴를 막기 위하여 농가에서 많이 사용되는 농가 지도형 G형 단동온실과 농가보급형인 1-2W 기본형 연동온실에 대해 시간에 따른 하중단계별 기하학적 비선형 효과를 고려하여 온실의 정확한 거동분석과 구조적 안전성을 평가하는데 대변위해석 방법을 제시하고자한다.