산화적 스트레스는 세포 및 조직 손상을 통해 피부의 탄력 및 보습 기능 저하, 피부 노화 촉진 을 비롯한 다양한 피부질환을 일으킨다. 본 연구의 목적은 인간 피부각질세포 (HaCaT keratinocyte)에서 산화적 스트레스에 대한 붉은 토끼풀 추출물의 효능을 검토하여, 피부에 효과적으로 사용할 수 있는 기능 성 소재로서의 활용 여부를 확인하고자 하였다. 본 연구에서는 붉은 토끼풀 추출물이 인간 피부각질세포에 서 산화적 스트레스에 따른 세포사를 억제시키는 것을 확인하여, 이를 조절하는 보호기전을 규명하였다. 이는 붉은 토끼풀 추출물이 Caspase-3 비활성, 세포사 촉진단백질 Bax 발현 억제, 세포생존 촉진단백질 Bcl-2 발현 증가 및 MAPK 신호전달계 단백질의 인산화 억제를 통해 H2O2에 의해 유도된 산화적 스트레 스를 보호할 수 있다는 것을 확인하였다. 따라서 붉은 토끼풀 추출물은 피부의 산화적 손상을 감소시키는 유용한 소재로 평가되며, 이는 피부보호 및 미용을 위한 다양한 제품 및 산업에 활용 가능성이 높은 것으로 판단된다.

In order to investigate the optimal UVB (ultraviolet B) treatment conditions for vitamin D2 enhancement of freeze-dried ear mushroom, sample size (below 300 μm~whole), UV treatment temperature (30~60℃), treatment density (6.25~50.0 mg/cm2) and the samples mixing frequency (1~32 times) were treated differently. After that, chromaticity, vitamin D2 and ergosterol (vitamin D2 precursor) contents were investigated. As a result of the investigating, effective UVB treatment conditions for vitamin D2 enhancement are as follows. The sample sizes were 2~4 mm and finely crushed pieces. The treatment temperatures were 50℃ and 60℃. The treatment density was 12.5 to 25.0 mg/cm2, and the number of sample mixing was 8 times or more. As the amount of vitamin D2 increased by UVB treatment, the ergosterol content generally tended to decrease. However, under some UVB treatment conditions, the vitamin D2 content was not high despite the decrease of ergosterol content. Under the conditions set in this experiment, it was possible to obtain ear mushrooms with enhanced vitamin D2 up to 26,968.7 μg/100 g. Therefore, it is thought that the ear mushroom is highly likely to be used as a vitamin D source and nutritionally fortified food ingredient.

The objective of this study is to evaluate the antioxidant components, elastase inhibition activities, and melanin synthesis rates of Korean traditional Actinidia (Actinidia arguta) fruits and leaves depending on the ethanol extraction concentrations. The total polyphenol content was the highest in the 50% ethanol extract of both fruits and leaves, with values of 634.1 mg GAE/100 g and 3,985.2 mg GAE/100 g, respectively. The total flavonoid content was the highest in the fruit 90% extract and leaf 50% extract at 191.9 mg/100 g and 2655.6 mg/100 g, respectively. The vitamin C content was the highest in the 50% extract of leaves at 2990.3 mg/100 g. Elastase inhibition was the highest at 56.9% in the leaf 50% extract at a concentration of 1,000 μg/mL. Melanin synthesis inhibition showed the highest melanin synthesis inhibitory effect among the extracts, as the leaf 50% extract showed an inhibitory rate of 65% or more. Therefore, the antioxidant components, elastase inhibition activities, and melanin synthesis inhibitory rate were better in leaves than in fruits. The leaf 50% extract was particularly the best among the extracts. Korean traditional Actinidia leaves can be considered as potential sources for new functional materials.

Seaweed-derived foods have long been popular in Korea because of their high content of nutrients that are beneficial to the human body. Recently, Korean seaweeds have been used as raw materials to produce new natural products with health benefits. Herein, we compared the antioxidant activity of 16 Korean seaweed extracts to explore their potential utility as health foods. The total phenolic content (TPC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of seaweed extracts were determined. We also investigated their ability to protect human diploid fibroblast (HDF) cells against hydrogen peroxide. The results showed that seaweed extracts at a concentration of 100 g/mL did not cause any cell toxicity. Sargassum thunbergii (Jichung-i) had the highest TPC and radical scavenging effects, followed by Porphyra tenera (Gim), Silvetia siliquosa (Tteumbugi), and Sargassum fusiforme (Tot). Hydrogen peroxide increased the production of intracellular reactive oxygen species, while P. tenera (Gim), Saccharina japonica (Dasima), and S. thunbergii (Jichung-i) extracts significantly decreased it. The effect was highest in the S. thunbergii (Jichung-i)-treated HDF cells. These findings indicate that S. thunbergii (Jichung-i) shows promise as a potential antioxidant raw material.

최근 기후변화로 가뭄, 홍수 등 재해의 빈도와 강도가 높아지고 있다. 이러한 재해의 피해를 줄이기 위해서는 시공간적 현황 파악을 통한 대비가 필요하다. 본 연구에서는 가뭄의 피해를 최소화하고자 지역별 밭작물의 필요수량과 공급량을 고려하여 밭가뭄 지역 등급화 실시하였고, 월별 비교를 통해 가뭄 취약 시기를 파악하였다. 전국 148개 지역 중 안전지역(Ⅰ), 안전지역(Ⅱ), 우려지역, 상습지역으로 구분한 25개 지역을 선정하였으며, 이 지역의 월별 필요수량 대비 공급량 분석을 하였다. 필요수량 산정의 재배작물은 콩으로 선정하였으며, 공급량은 공공관정, 민간관정, 상수도 자료를 분석하였다. 필요수량 대비 공급량이 봄철은 안전지역(Ⅰ), 안전지역(Ⅱ), 우려지역, 상습지역에서 각 1,281.5%, 667.6%, 729.5%, 316.3%, 가을철에는 각 436.0%, 212.8%, 213.9%, 105.3%로 공급이 충분할 것으로 분석되었으나 여름철에는 각 82.4%, 40.6%, 42.6%, 20.0%로 용수공급 이 부족한 것으로 분석되었다. 콩의 재배 기간인 5~9월에 관정(공공+민간)으로의 공급량은 대부분 지역에서 부족한 것으로 분석되었다. 이러한 분석을 통해 용수 부족이 발생하는 재배기간 동안 용수확보 방안이 필요하다.

Centella asiatica (C. asiatica) has been widely used in food, cosmetics, and pharmaceutical industry as a functional material. In a previous study, we have investigated not only pharmacological effects such as antioxidative and anti-inflammatory effects, but also analyzed various functional ingredients. In this study, triterpenoids were analyzed using HPLC-DAD to determine marker compounds among functional ingredients. When triterpenoids were analyzed, asiaticoside from C. asiatica was determined as an optimal marker compound. Next, specificity, linearity, limited of detection (LOD), limited of quantification (LOQ), precision, accuracy, and range were evaluated using HPLC-DAD to determine asiaticoside contents in C. asiatica juice and extracts. The specificity was elucidated by chromatogram and retention time using an established analytical method. The coefficient of correlation obtained was 0.9996. LOD was 4.99 μg/mL and LOQ was 15.12 μg/mL. Intra- and inter-day precision of asiaticoside were determined to be 0.48~1.68% and 0.08~1.09%, respectively. Furthermore, the recovery rate of asiaticoside was 98.88% and the analytical range of Field-70E was determined to be 0.625~10 mg/mL. As a results of evaluating ABTS, DPPH, and FRAP antioxidative effect, Field-70E showed potent antioxidant activities. Results of this study could be used as basic data for quality standardization of C. astiatica juice and extracts.

This study was conducted to apply with an air duct for the cooling and a utilizing cultivating method that uses the fruiting node and the defoliation to the high-temperature vertical and hydroponic cultivation of the oriental melon. The lower fruiting node (LF) was to remove all third vines generated from 5 nodes of a secondary vine. The higher fruiting node (HF) was fruiting on the third vine generated from a first node of the third vine. The direction of the stem string; upward (UW), downward (DW). Four treatment conditions were applied with the LF-UW, LF-DW, HF-UW (control), and HF-DW. The leaf age of melon leaves was measured for photosynthesis at 3 days intervals, and the fruit characteristic was conducted on 79 fruits in each treatment. The photosynthesis rate steadily increased after leaf development, reaching 20.8 μmol CO2·m-2·s-1 on the 10 days, gradually increasing to 21.3 μmol CO2·m-2·s-1 on the 19 days, and reaching 23.4 μmol CO2·m-2·s-1 on the 32 days. After that, it lowered to 16.8 μmol CO2·m-2·s-1 on the 38 days and dropped significantly to 7.6 μmol CO2·m-2·s-1 on the 47 days. As a result of the fruit characteristics by fruiting nodes, the treatments of the fruit length was 12.6-13.4 cm, respectively, which was significant, and the fruit width was 7.9- 8.6 cm, respectively, was not significant. The soluble content ranged from 12.9 to 15.7°Brix, and the significance of all treatments, and higher than of LF-DW and HF-UW. The photosynthesis rate of melon leaves was good until 32 days after leaf development, but after that, the rate decreased. As for fruit quality, it was conformed that melons can be cultivated at the LF because the fruit enlargement and soluble content dose not decrease even when set at the LF. Results indicated that those can be used for LF and defoliation in the development of vertical and hydroponic cultivation method in high-temperature season.

본 연구는 예비노인세대를 대상으로 가족관계와 고용차별의 관계에서 연령주의의 매개효과를 검증하는 것이다. 이를 위하여 A시에 거주하는 예 비노인(54세~64세)에게 면접식 설문조사를 실시하여 총 509부를 분석에 사 용하였다. 자료분석은 SPSS 25.0 프로그램을 사용하여 기술통계분석, 각 변 인 간 영향력 검증을 위한 중다회귀분석, 연령주의의 매개효과를 알아보기 위한 경로분석을 실시하였다. 주요 연구결과는 다음과 같다. 예비노인세대 의 연령주의는 가족관계와 고용차별에 미치는 영향에서 완전매개효과가 있었다. 이는 가족관계와 고용차별에서 연령주의가 매우 중요한 역할을 한다고 해석할 수 있다. 이러한 결과는 예비노인세대의 가족관계를 강화함 으로써 이들의 삶에서 매우 중요한 역할인 일과 관련된 주요변수인 고용차 별을 완화할 수 있고, 가족관계 수준이 낮더라도 연령주의를 다양한 경로로 활용한다면 예비노인이 경험하는 노동이라는 특정영역에서의 고용차별을 완 화할 수 있다는 것을 확인한 것이다. 이러한 통합적 경로확인을 바탕으로 예 비노인세대가 역할 축적과 가정-일을 풍요롭게 영위할 수 있도록 매우 고령 까지 경험할 가능성이 있는 고용차별을 낮추거나 예방할 수 있는 다양한 방 법을 모색하였다.

This study was conducted to investigate the vertical distribution and vascular plants in the Gakho mountain. Form the results of three field surveys from May 2022 to September 2022, a total of 478 total taxa, representing 426 species, 11 subspecies, 35 varieties, four forms, and two hybrids were identified, which were categorized in 282 genera and 94 families. We identified the elevational distribution ranges of 398 taxa of vascular plants. Among them, 19 taxa were endemic to Korea, one taxon was identified as a rare plant. The floristic target plants amounted to 72 taxa, specifically two taxa of grade V, two taxa of grade IV, 16 taxa of grade III, 27 taxa of grade II, and 25 taxa of grade I. Further, 71 taxa were identified as northern lineage plants. A total of 19 taxa of alien plants were identified, with a Naturalized Index of 4.0%, an Urbanization Index of 6.6%, and three plants that disturbed the ecosystem. The result of analyzing the pattern of species richness showed a reversed hump shape with minimum richness at midhigh elevation. A cluster analysis showed a high degree of similarity between adjacent elevation sections that are geographically adjacent with similar habitat environments. Warmth index in the Gakho mountain ranged from 57.2°C · month to 84.2°C · month. Our results provide basic data on vascular plants and valuable information on elevational distribution ranges of current plant species in the Gakho mountain, which could serve as a baseline for comparison of the shifts in elevation under future climate change.

The present study investigated the effects of forage cutting and baler mixing on the chemical compositions, fermentation indices, and aerobic stability of whole crop rice (WCR) haylage. The WCR (“Youngwoo”) was harvested at 48.4% dry matter and ensiled into a 300 kg bale silo with forage cutting (whole crop without cutting vs. 5 cm of cutting length). The WCR forages were ensiled without baler mixing process (CON) or with (MIX). The concentrations of dry matter, crude protein, ether extract, crude ash, neutral detergent fiber, and acid detergent fiber of whole crop rice before ensiling were 48.4, 9.70, 2.57, 6.11, 41.2, and 23.5%, respectively. The forage cutting did not affect the chemical compositions, fermentation indices, microbes, and aerobic stability of WCR haylage (p>0.05). The CON haylages tend to be higher in NDF content (p<0.10). The MIX haylages had lower in lactate (p=0.019), and lactate:acetate ratio (p<0.001). The MIX haylages had higher in lactic acid bacteria (LAB) (p=0.010). Therefore, this study concluded that the fermentation quality of WCR haylage improved by baler mixing, but had no effects by forage cutting.

목적 : 본 연구는 대한작업치료학회지에 게재된 논문을 중심으로 한국 작업치료의 연구 동향을 파악하고자 하였다. 연구방법 : 대한작업치료학회지에 2016년부터 2020년까지 게재된 213편의 논문을 대상으로 연구 유형, 근거수준, 연구 분야, 1 저자 정보, 연구 대상자, 연구 변인의 유형을 분석하였다. 결과 : 분석 결과로 연구 유형은 기술적 연구가 33.80%로 가장 많았고, 근거수준 중 수준 4가 47.89%로 가장 높았으며, 2016년에 비해 수준 1과 수준 4의 연구 수가 증가한 것으로 나타났다. 연구 분야는 작업 치료일반이 28.64%로 높게 나타났으며, 게재된 논문의 1 저자 직위는 대학원생이 33.64%로 가장 높았 다. 실제 연구 대상자가 포함된 연구 중 성인을 대상으로 한 연구가 54.95%로 가장 많았으며, 연구 대상 자 진단명은 뇌졸중이 가장 높았다. 연구 변인은 기술적 연구에서 작업/활동 기반 작업치료가 15.28%였 고, 실험연구의 독립변인은 작업/활동 기반 작업치료가 28.30%, 종속변인은 운동기능이 26.36%로 높게 나타났다. 결론 : 본 연구를 통해 한국 작업치료의 연구의 근거수준의 향상과 연구분야의 다양성을 확인할 수 있었 다. 이에 본 연구 결과가 향후 연구 방향의 설정에 신뢰할 만한 자료로 활용되길 기대한다.

Following the social requirement to strengthen field supervision of the asbestos containing materials (ACM) abatement process with regard to asbestos school buildings, this study was conducted to understand the status and characteristics of airborne asbestos that may potentially occur after the ACM abatement process is completed. In the area where a series of asbestos abatement processes were finally completed, comprehensive area air sampling was performed. For sample analysis, Transmission Electron Microscopy (TEM) was used according to The Asbestos Hazard Emergency Response Act (AHERA) method and Phase Contrast Microscopy (PCM) analysis was also performed. Airborne asbestos was detected in 29.5% of the total samples, and the average concentration was 0.0039 ± 0.0123 s/cc (12.3 ± 38.9 s/mm2). 4.5% of the total samples exceeded the AHERA standard (70.0 s/mm2) and the average concentration was 0.0528 ± 0.0256 s/cc (167.2 ± 82.0 s/mm2). Airborne asbestos was no longer detected at the point when AHERA is exceeded after re-cleaning. Most of the detected asbestos was chrysotile (94.4%) and the structure types of asbestos were Matrix (41.4%), Fiber (39.9%), Bundle (10.8%), and Cluster (7.8%). Among the asbestos structures detected through transmission electron microscope analysis, the asbestos structures satisfying PCM-equivalent structures were found to be 6% of the detected asbestos, indicating that there is a limitation of the PCM analysis to check the airborne asbestos in that area. As a result of reviewing the status of airborne asbestos that may potentially occur and the type and dimensions of asbestos structure detected in the area, since the airborne asbestos exposure caused by poor field supervision for the ACM abatement process could not be ruled out, thorough management is necessary. In addition, the result of this study could be used as scientific evidence for establishing and strengthening policies related to ACM abatement, including cases of school buildings.