최근 국내에서 개발된 송풍식 양파 줄기절단기를 수확기 양 파 엽 절단에 활용할 경우 적정 작업 조건을 구명하기 위해서 이 실험을 수행하였다. 처리구 중 식물체 엽 건조가 가장 많이 진행된(엽 건조 정도 : 66.3%, 엽 수분함량 : 50.5%) 6월 20일 엽 절단 처리구에서 평균 잔여 엽장은 6.7±3.5cm로서 작업 후 적정 잔여 엽장에 해당되는 범위인 4－10cm에 포함되므로 기계 엽 절단 성능이 처리구 중 가장 우수한 것으로 판단된다. 줄기절단기 이용 양파 엽 기계 절단 시 평균 작업 속도는 0.17m·s-1였는데, 이는 인력 엽 절단 처리구의 평균 작업 속도 인 0.05m·s-1보다 3.4배 정도 빨랐으며, 이를 통해 해당 기종 을 이용하여 10a 면적을 작업할 경우에는 인력 작업(1인 기준) 에 비해 2.6시간 정도를 절감할 수 있을 것으로 기대된다. 또한 기계 엽 절단 처리구에서의 손상구 발생률은 1.3%로서 인력 엽 절단 처리구의 0.0%에 비해 높았는데, 이로 인해 기계 엽 절단 처리구가 인력 엽 절단 처리구보다 저장 중 양파 구 부패 율이 평균적으로 높았던 것으로 판단된다. 처리별 저장 특성 을 살펴보면, 저장 8개월 후 구 부위(기부, 정부)별 부패율은 기계 엽 절단 후 잔여 엽장이 5.0cm 미만인 처리구에서 잔여 엽장이 5.0cm 이상인 처리구보다 높았다. 이는 잔여 엽 길이 가 5.0cm 미만인 처리구에서는 5.0cm 이상인 처리구보다 저 장 중 구 부패를 유발하는 병원균의 감염이 쉬우므로 장기 저 장 시 부패율이 높은 것으로 생각된다. 본 실험 결과와 실험 기 종의 성능 목표(작업 후 잔여 엽장 : 5cm) 및 양파 수확 시 적정 잔여 엽장에 관한 기존의 연구결과 등을 종합적으로 고려할 경우, 본 실험에 사용된 줄기절단기 이용 양파 엽 절단 시 양파 의 적정 잔여 엽장은 5－10cm 정도일 것으로 판단된다.

Dissolved organic matter (DOM) is a key component in the biogeochemical cycling in freshwater ecosystem. However, it has been rarely explored, particularly complex river watershed dominated by natural and anthropogenic sources, such as various effluent facility and livestock. The current research developed a new analytical method for TOC/TN (Total Organic Carbon/Total Nitrogen) stable isotope ratio, and distinguish DOM source using stable isotope value (δ13C-DOC) and spectroscopic indices (fluorescence index [FI] and biological index [BIX]). The TOC/TN-IR/MS analytical system was optimized and precision and accuracy were secured using two international standards (IAEA-600 Caffein, IAEA-CH-6 Sucrose). As a result of controlling the instrumental conditions to enable TOC stable isotope analysis even in low-concentration environmental samples (<1 mgC L-1), the minimum detection limit was improved. The 12 potential DOM source were collected from watershed, which includes top-soils, groundwater, plant group (fallen leaves, riparian plants, suspended algae) and effluent group (pig and cow livestock, agricultural land, urban, industry facility, swine facility and wastewater treatment facilities). As a result of comparing characteristics between 12 sources using spectroscopic indices and δ13C-DOC values, it were divided into four groups according to their characteristics as a respective DOM sources. The current study established the TOC/TN stable isotope analyses system for the first time in Korea, and found that spectroscopic indices and δ13C-DOC are very useful tool to trace the origin of organic matter in the aquatic environments through library database.

Conventional aquaculture faces declining productivity, shifting to recirculating aquaculture system (RAS), known for minimizing water usage and maintaining consistent water temperatures for year-round fish growth. Rainbow trout (Oncorhynchus mykiss), a globally important cold-water species and the third most farmed fish in inland waters of Korea, valued for its fecundity and rapid growth. Dissolved oxygen, an important environmental factor affecting fish production and economics, highlights the need for smart aquaculture practices. Since 2018, the rise of intelligent aquaculture platforms, incorporating information and communications technology (ICT), emphasizes the essential role of RAS implementation. This eight-week study aimed to determine the optimal dissolved oxygen concentration for rainbow trout in RAS, utilizing a device for continuous monitoring, control and record. Dissolved oxygen concentrations were set at 5-6 mg/L, 9-10 mg/L, 14-15 mg/L and 17-18 mg/L. The growth rate significantly decreased at 5-6 mg/L, with no significant differences in other experimental groups. In hematological analysis, growth hormone (GH) was significantly highest at 5-6 mg/L, followed by 9-10 mg/L while Insulin-like growth factor-1 (IGF-1) was significantly lowest at 5-6 mg/L. In conclusion, the optimal dissolved oxygen concentration for rainbow trout in RAS is approximately 9-10 mg/L. Higher concentrations do not contribute to further growth or profitability.

This study investigated the nutritional characteristics of before and after fermentation of domestic soybean (Glycine max L.) by Rhizopus oligosporus. The soybean storage proteins, β-conglycinin (11S globulin) and glycinin (7S globulin), were the most abundant in Seonyu (SY) and Danbaegkong (DBK), with concentrations of 253.4 mg/g and 193.0 mg/g, respectively. For 11S/7S related to sulfur-containing amino acid, DBK had a value of 0.95, making it the most excellent nutritionally among all the cultivars. The free amino acid content significantly increased from 0.04~10.45 mg/g before fermentation to 1.37~16.95 mg/g after fermentation, and the essential amino acid composition increased, confirming an improvement in protein quality after fermentation. Phytic acid, known as a nutritional inhibitor of soybeans, decreased from 1.66~2.13 g/100 g before fermentation to 0.90~1.58 g/100 g after fermentation, suggesting that mineral absorption inhibition was alleviated. In addition, the trypsin inhibitor content is suppressed by 76.20% to 81.25% after fermentation, which is expected to improve protein utilization in the body. This study confirmed some properties of fermented products by Rhizopus oligosporus using domestic soybeans, and these results are presented to serve as the basic data for establishing new uses of Korean soybean cultivars.

The compositions of ethnic groups in Macau vary with time. Prior to the opening of the port, the majority of the residents in Macau were Chinese people, including those living on land and at sea. After the port was opened, with the increase of Portugal businessmen and missionaries, the population was divided into Chinese people and foreigners (so-called ‘Yiren’ or 夷人in Chinese). Chinese people living on land were mainly of Hakka, Fujian, and Cantonese descent. Those living at sea were referred to as ‘Tanka People’ (named ‘Danmin’ or 蜑民in Chinese). They lived on floating boats for their entire lives and were similar to the ‘drifters’ in Japan. Since modern times, many refugees from mainland China and Southeast Asia flooded into Macau due to warfare. The development of industrialization required a larger number of laborers, and some ‘coolies’ entered Macau in legal or illegal ways, making it a multi-ethnic city. However, the Tanka people were not considered a minority ethnic group under the national ethnic policy of 56 ethnic groups since they did not have an exclusive language and shared dialects in different regions. As the ports inhabited by Tanka people gradually restored foreign trade, the boats and stilt houses used by Tanka people were dismantled to expand the infrastructure area of the ports. Many Tanka people began to live on land and marry people on land, leading to the disappearance of the Tanka group in Macau. The fishing boats and stilt houses used by Tanka people have also disappeared, with only a few remaining in areas such as Pearl River Delta and Hong Kong. This paper examines the natural and social environment of Tanka people in Macau from the Ming and Qing dynasties to the Republic of China, as well as the adaptive changes they adopted for the aforementioned environment in terms of living space and architectural type, on the basis of summarizing the historical activities of Tanka people. Finally, this study provides a layout plan and interior structure of the most commonly used boat for Tanka people from the Ming and Qing dynasties to the Republic of China, with the use of CAD and other technical software, along with reference to written historical documentation, and provides a case study for further research on the architectural history of Macau's inner harbor cities, from anthropological and folklore perspectives.

산화적 스트레스는 세포 및 조직 손상을 통해 피부의 탄력 및 보습 기능 저하, 피부 노화 촉진 을 비롯한 다양한 피부질환을 일으킨다. 본 연구의 목적은 인간 피부각질세포 (HaCaT keratinocyte)에서 산화적 스트레스에 대한 붉은 토끼풀 추출물의 효능을 검토하여, 피부에 효과적으로 사용할 수 있는 기능 성 소재로서의 활용 여부를 확인하고자 하였다. 본 연구에서는 붉은 토끼풀 추출물이 인간 피부각질세포에 서 산화적 스트레스에 따른 세포사를 억제시키는 것을 확인하여, 이를 조절하는 보호기전을 규명하였다. 이는 붉은 토끼풀 추출물이 Caspase-3 비활성, 세포사 촉진단백질 Bax 발현 억제, 세포생존 촉진단백질 Bcl-2 발현 증가 및 MAPK 신호전달계 단백질의 인산화 억제를 통해 H2O2에 의해 유도된 산화적 스트레 스를 보호할 수 있다는 것을 확인하였다. 따라서 붉은 토끼풀 추출물은 피부의 산화적 손상을 감소시키는 유용한 소재로 평가되며, 이는 피부보호 및 미용을 위한 다양한 제품 및 산업에 활용 가능성이 높은 것으로 판단된다.

The construction method of scaffolding structures is different from Mortise and Tenon and bucket arch structure of traditional large woodwork. It forms an independent construction system-fixing nodes with knots, a large number of diagonal braces are used to fix shelves and the structures mostly contain X-shape and triangular shape details. Simple ones include stalls, sheds, rain sheds, altars, lamp racks etc. But the scaffolding with larger scale and more complicated structure are modeled on archways, theatres and other buildings which are used in commercial and festival activities. At present, Macao, Hong Kong, Guangdong, Sichuan, Shanxi and other places in China have retained the custom of using scaffolding structures in important festival activities, but their uses, techniques and building types are slightly different from place to place. Due to building and demolishing at any time, the construction and service cycle is short. As a result, there are almost no physical objects left. We can only deduce the use and technical characteristics of ancient scaffolding skills through the colorful building styles that have been preserved with folk activities in various parts of China, the craftsmanship handed down from generation to generation by the scaffolding guild and artisans, and the description of cultural and historical materials and the mutual corroboration of visual materials.

In vitro digestibility and protein digestibility corrected amino acid scores (PDCAAS) were investigated to verify the availability of protein in various Rhizopus oligosporus fermented products of domestic soybean (Glycine max L.) cultivars. Danbaegkong (DBK), Daepung (DP), Daewonkong (DWK), Saedanbaek (SDB), Seonyu (SY), and Cheongja4ho (CJ4) were used as raw samples, which were fermented using commercially available Rhizopus oligosporus for 48 h. All cultivars showed increased crude protein content after fermentation. The crude protein content of DBK and SDB was significantly higher than that of the other samples (55.12% in DBK and 54.22% in SDB) (p<0.001). CJ4 had the highest alanine content of 28.88 mg/g (p<0.001), and no significant difference in cysteine content was detected among the cultivars. In most of the fermented samples, the in vitro digestibility was 0.9 or higher, indicating high protein in the fermented samples. However, it is considered that restrictions on digestion are low. In DWK, the amino acid content and PDCAAS, which together indicate protein quality, were 0.917 and 0.855, respectively, confirming that it was the best cultivar to provide the raw material for fermentation. In conclusion Rhizopus oligosporus fermented soybean products can be considered a prospective source of protein with high utility value.

바이오산업의 발전으로 의약품, 식품 등의 생산 과정의 분리/정제 공정에 사용되어 왔던 기존의 컬럼 크로마토그 래피를 대체하여 더 높은 처리효율을 갖는 막 크로마토그래피가 부상하고 있다. 본 연구에서는 서로 다른 기공 크기의 두 가 지 상용 셀룰로오스 아세테이트(Cellulose acetate, CA) 분리막을 탈아세틸화 과정을 통해, 리간드의 개질이 용이한 다공성 재 생 셀룰로오스 지지체를(Regenerated cellulose, RC) 제조하였다. 음이온 교환능을 부여하고자 grafting을 수행하였으며, 구체 적으로는 UV 중합법을 통해 4차 암모늄을 포함하는 음이온 교환 리간드(MAPTAC)를 부착하여 음이온 교환용 흡착막을 제 조하였다. 단백질 흡착 용량은 정적 흡착 용량(Static binding capacity, SBC)시험을 통해 총 단백질 흡착 용량을 측정했고, 동 적 흡착 용량(Dynamic binding capacity, DBC)을 측정하여 상용막과 비교 평가하였다. 성능 평가 결과 단백질 흡착량은 넓은 표면적에 의해 리간드 밀도가 높은, 기공 크기가 작은 순서로 높게 측정되었고, 상용 CA분리막을 탈아세틸화하고 리간드를 부착시킨 분리막(RC 0.8 + MAPTAC 43.69 mg/ml, RC 3.0 + MAPTAC 36.33 mg/ml)이 상용 막 크로마토그래피 제품(28.38 mg/ml) 대비 높은 흡착 용량을 보였다.

PURPOSE: 본 연구의 목적은 둥근어깨자세(round shoulder posture)와 전방머리자세 (forward head posture)에 따라 폐활량 측정과 산소포화도 및 횡격막 움직임 크기의 상관관계를 알아보는 것이다. METHODS: 연구의 대상은 만성 심장질환 및 호흡기 질환을 가지고 있거나, 정신적, 인지적 장애가 있는 자, 척추옆굽음증(scoliosis)이 있는 자, 목과 갈비뼈 손상이나 수술 병력이 없는 50명의 자원한 사람으로 선정되었습니다. 통계분석은 피어슨(Pearson)의 상관분석을 실시한다. RESULTS:둥근어깨자세는 FVC,FEV1,FEV1/FVC%,PEF 에 대해 양의 상관계수를 보이고 이중 PEF가 가장 상관성이 높았다(p<.05). 횡격막 움직임의 크기(DMD)에 대해서도 양의 상관계수를 보였다. 그러나 TAD(table acromion distance)와는 음의 상관관계를, SPO2는 PM/C7~acro, TAD(table acromion distance) 둘다 음의 상관관계가 나타났다(P>.05). 전방머리자세는 CVA와 CRA 모두 FVC,FEV1,FEV1/FVC, PEF 비율에 대해 유의한 상관관계가 없다(p>.05). CRA와 DMD에는 유의한 차이가 있는 음의 상관관계가 나타났지만(p<.05), CVA와 DMD에는 유의한 상관관계가 발견되지 않았다. 또한 CVA와 CRA 모두 SPO2에 대해 유의한 상관관계가 없다(p>.05). CONLUSION: 결론적으로 전방머리자세의 각도와 둥근어깨 자세에 따라 폐활량과 횡격막 움직임에 영향을 미친다는 것을 확인 할 수 있었다. 하지만 좀더 많은 인원과 대상자들의 경직된 자세에 대해 제한할 방법을 추가적으로 찾을 필요가 있다.