에너지 음료는 카페인을 주성분으로 타우린, 비타민 같은 다른 energy-enhancing 성분을 함유하고 있다. 미국과 유럽에서는 글루쿠로노락톤이 에너지 음료에 첨가될수 있으나, 국내에서 의약품으로는 허가되어 있다. 따라서 식품 첨가물로는 그 사용이 금지 되어 있어, 지속적으로 수입 및 유통 음료에서 시험검사를 하여 규제하고 있다. 현재 분석법으로 사용하는 LC-PDA 법은 복잡한 유도체화 과 정을 거치고, 음료 중에 당류들이 위양성 결과를 나타내 기도 한다. 이런 기존 방법의 단점을 개선하기 위해 HILICESI- MS/MS (hydrophilic interaction liquid chromatography coupled to electrospray ionization tandem mass spectrometry) 를 이용한 분석법을 개발하고, 선택성, 직선성, 검출한계, 정량한계, 정밀도, 정확성, 재현성에 대하여 분석법 유효성 검증을 수행했고, AOAC, EURACHEM 가이드라인에 부합되는 결과를 얻었다.

The native human saliva obtained through the centrifugation of whole saliva showed characteristic salivary protein complex (SPC) peaks in gel filtration high performance liquid chromatography (HPLC) using Superose 12 column1,2). In the previous study the SPC peaks in chromatography were explored to know their composition and functions by different detection methods, but still the nature of SPCs was not clearly elucidated so far. In this study the SPC peaks were examined by direct antibody interaction in order to target different antimicrobial and protective proteins distributed in the SPCs via gel filtration HPLC. As the SPC peak shape and migration speed can be changed by antibody binding to specific proteins of SPC, it was found that mucin1 is evenly distribution in all SPCs, while PRPs are more abundant in the late dominant SPC than the early dominant SPC and also in the intermediated SPCs. Most of antimicrobial proteins including lysozyme, LL-37, lactoferrin, β-defensin-1, -2, -3, IgA, mucocidin, and α1-antitrypsin were more abundant in the late dominant SPC than the early dominant SPC, while histatin showed relatively even distribution in all SPCs. Therefore, it was presumed that the late dominant SPC containing abundant antimicrobial and protective proteins could be applied as a biomarker to measure the defensive potential of whole saliva in oral diseases.

LC-MS/MS를 이용하여 폴리펩타이드계 동물용의약품인 콜리스틴에 대한 시험법을 확립하여 정량성 및 정밀성을 확보하였으며, 확립된 시험법의 적용성 검증을 위해 국제식품규격위원회 기준에 따라 특이성, 정확성, 직선성, 정밀성, 검출한계, 정량한계 등을 검증하였다. 콜리스틴 표준용액을 잔류허용기준의 농도에 따라 검량선을 작성한 결과 0.99 이상의 직선성을 확인 할 수 있었으며, 본 실험에서의 평균 회수율은 85.9~107%이었다. 또한, 분석오차는 11.8% 이하로 정확성 및 재현성이 우수하였으며, 검출한계는 0.02 mg/kg, 정량한계는 0.05 mg/kg이었다. 또한, 실험실간 교차검증을 통하여 신뢰성을 확보하였다. 확립된 분석법은 양식 수산물 중 잔류할 수 있는 동물용의약품인콜리스틴에 대한 안전관리에 활용 할 수 있을 것으로 판단된다.

Various methods for the detection of E. coli O157:H7 in food have been developed in the past decades. However, current detection methods require specialized instruments and lengthy preparation time. In an effort to achieve a rapid and sensitive detection, we developed a radial chromatography (RC) biosensor integrated with gold nanoparticle (AuNP) conjugated with antibody for the detection of E. coli O157:H7. The immuno-AuNP binds to E. coli O157:H7 creating AuNP-E. coli O157:H7 complexes by specific antigen-antibody interaction. The AuNP-E. coli O157:H7 complexes can be identified clearly from free AuNP by RC based on their mobility on porous matrix. Thus, the AuNP complexed with target bacteria, E. coli O157:H7 could be discriminated from free AuNP by radial chromatography. The results showed that the developed RC biosensor is highly selective to E. coli O157:H7 over non-target bacteria, including Bacillus cereus, Staphylococcus aureus and Vibrio cholerae with a detection limit of 105 CFU/ml. When combined with a pre-concentration step using immunomagnetic beads, we could further enhance the detection limit down to 103 CFU/ml. In this study, we developed a novel method that is rapid, sensitive and applicable for qualitative and quantitative detection of E. coli O157:H7. The detection procedure is simple and the results can be easily determined by naked eyes, suggesting that this system is practical and can be applied to the field diagnosis in food industry for the detection of pathogenic bacteria.

선행연구에서 화장품 소재로서 보습력과 방부력을 가지고 있는 1, 2-hexanediol (HD)의 transgalactosylation 반응을 통하여 galactose한 분자가 HD에 결합한 1, 2-hexanediol galactoside (HD-gal)의 합성을 확인하였다. 본 연구에서 재조합 β-galactosidase (β-gal)가 발현된 Escherichia coli (E. coli) 세포를 이용하여 약 94%의 수율로 HD-gal가 합성되는 것을 관찰하였고, HD-gal을 합 성한 후, 보다 효과적인 HD-gal의 정제 방법에 대하여서도 연구하였다. 먼저 고농도의 lactose (300 g/l) 존재 하에서 β-gal을 함유한 E. coli 세포를 이용하여, 48 시간 동안 75 mM의 HD로 부터 HD-gal이 합성되는 것을 TLC 분석으로 확인하였고, 반응액에서 E. coli β-gal의 존재를 Western blotting으로 확인할 수 있었다. HD-gal을 효과적으로 순수 정제하기 위하여, 용매를 사용하여 transgalactosylation 반응이 끝난 후 잔여 HD를 우선 제거하고, 이어서 silica gel chromatography를 수 행하는 방법을 실시하였다. 물에 녹지 않는 용매로는 methylene chloride와 ethyl acetate를 선택하여 비 교 실험하였는데, ethyl acetate를 사용하여 4회 물층을 분획하여, 잔여 HD를 효과적으로 제거할 수 있 었다. 그 후, 이어서 silica gel chromatography 수행하여, 순수한 HD-gal을 효과적으로 정제하였다. 반 응에 첨가된 75 mM의 HD를 기준으로 최종 정제된 HD-gal의 생산 수율은 mole 기준으로는 약 8.9±0.6% (n=3), weight 기준으로 약 21.1±1.4% (n=3) 정도였다. 앞으로 이러한 정제 방법을 이용하 여 얻은 HD-gal의 항균력 변화를 HD와 비교하여 평가할 예정이고, 피부세포에 대한 독성 변화를 역시 HD와 비교하여 분석할 예정이다.

Choline is an amine essential to maintenance of good health. Here, we modified the ion chromatographic method for measuring choline described by Laikhtman & Rohrer and validated by the ICH guideline and applied it to monitoring of choline content in infant formula in the Republic of Korea. The Certified Reference Materials (CRM), NIST 1849a, were used for validation of the modified method. The parameters of validation were linearity, range, specificity, accuracy, precision, LOD, LOQ and recovery. The linearity, R2, was above 0.999 and the analytical range was 2.5 to 50 μg/mL. The specificity was confirmed by the retention time (RT) and positive tests with calibration standard and CRM. The accuracy, precision and recovery values were 99.6 ± 2.41 %, 2.42 % and 90.8 - 92.8 %. The LOD and LOQ values were 0.03 mg/kg and 0.17 mg/kg, respectively. Five livestock products inspection agencies voluntarily participated in the collaborative study. The result, HORRAT value was 0.8 which was within the reference value 0.5 – 2.0. The validated method was applied to the analysis of choline in infant formula in the Republic of Korea. The results also conformed to 'The Standard for Livestock Products Labeling'.

In this study, we developed and validated microanalysis methods for the determination of linear alkylbenzenesulfonate (LAS), sodium lauryl sulfate (SLS), and alpha olefin sulfonate (AOS). The conditions for the analysis of the surfactants using HPLC with FLD, RID, and ELSD detectors were investigated. The methods were validated by determining the linearity, limits of detection (LODs), limits of quantification (LOQs), recovery, precision, and accuracy. LAS analysis by FLD revealed calibration curves that were linear in the range of 10-200 mg/L for an LAS mixture. The calibration curves for C10-C13 had correlation coefficients of 0.995, 0.997, 0.996, and 0.997, respectively. SLS analysis using RID generated a linear calibration curve in the range of 10-300 mg/L. The calibration curve for SLS C12 had a correlation coefficient of 0.9994. AOS analysis using ELSD resulted in a correlation coefficient of 0.9940. For LAS, the LODs and LOQs were 0.09-0.56 and 0.30-1.87 mg/L, respectively. For SLS C12, the LOD and LOQ were 0.07 and 2.33 mg/L, respectively. For AOS C14, the LOD and LOQ were 16.55 and 21.83 mg/L, respectively. The recoveries were 97.17-98.84% for LAS C10-C14, 97.94% for SLS C12, and 96.11% for AOS C14. The established methods provide acceptable precision and accuracy. Our methods could be useful for the detection of anionic surfactants in dishwashing detergents.

Mycotoxins, such as aflatoxin B1 (AFB1), deoxynivalenol (DON), fumonisin B1 (FMB1), ochratoxin A, T2 toxin, and zearalenone, are found in numerous vegetables. Mycotoxin accumulation in food and feed poses serious health risks to humans and animals because of carcinogenic, mutagenic, teratogenic, and toxic properties. In addition, mycotoxins cause large economic losses in commercial crop production, food and feed processing, and animal husbandry worldwide. In this study, an analytical method for the simultaneous analysis of the levels of AFB1, DON, and FMB1 in cow blood with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated. AOZTM and Myco6in1TM multitoxin immunoaffinity columns and an OasisTM reversed-phase solid-phase extraction Hydrophilic-Lipophilic-Balanced columns were used to purify and concentrate the blood samples. Extracts that contained AFB1, DON, and FMB1 had average recovery of 64.0%, 98.0%, and 89.9%, respectively. In conclusion, we used LC-MS/MS to detect several important toxicological mycotoxins in cow blood. The multimycotoxin method, which detected and quantified the levels of AFB1, DON, and FMB1 can be used in animal pilot studies to monitor simultaneous exposure to major mycotoxins.

Immunoprecipitation-based high performance liquid chromatography (IP-HPLC) is a type of modified enzyme-linked immunosorbent assay (ELISA) that uses protein A/G (or antibody)-conjugated beads instead of the antibody-conjugated wells used in ELISA. In order to determine the fidelity of IP-HPLC, the author used 83 antisera to identify protein expression changes caused by cisplatin treatment in KB human oral cancer cells. KB cells were cultured for 12 or 24 hours with 10 ug/mL cisplatin. The results obtained by IP-HPLC were comparable with published cisplatin data, although ELISA was not conducted in the present study. Cisplatin dominantly reduced the levels of proteins associated with cell proliferation, transcription factors, growth factors, cytoskeletal proteins, and cellular differentiating factors, but on the other hand, apoptosis-related factors, oncogenes, and protective proteins were usually up-regulated, presumably to address cisplatin-induced DNA damage. In particular, cisplatin directly inactivated genomic DNA by down-regulating histone H1 and demethylase and by up-regulating deacetylase. Cisplatin also rapidly induced p53 overexpression and mitochondria-mediated endogenous apoptosis occurred after 12 hours of cisplatin treatment, although this was almost completely replaced by FASL/FAS-mediated exogenous apoptosis after 24 hours. This preliminary study was conducted to investigate the anticancer effect of cisplatin on the KB human oral cancer cells and to determine the fidelity of IP-HPLC data. It was concluded that IP-HPLC is useful for identifying profile changes of genome wide essential proteins and signaling changes of major molecular pathways.

황색포도상구균 식중독은 해당 균이 세포 밖으로 분비 하는 장독소에 의해 발병되며, 지금까지 밝혀진 16가지 독소 중 내열성이 가장 강하고 흡입중독으로 쉽게 질환을 일으킬 수 있는 Staphylococcal enterotoxin B형을 분리해 내는 방법에 대해 연구하였다. 분자량 26-29 kDa의 단순 단백질인 SEB를 분리 및 정제하기 위한 방법으로 fast protein liquid chromatography (FPLC)를 이용하였으며, 정제율을 높이기 위해 ion exchange chromatography와 gel filtration chromatography의 두 과정을 거쳤다. 실험 결과 SEB의 분리 조건은 CIEX에서 WorkBeads 40S컬럼 의 분리능이 가장 좋았고, 염 농도 15%에서 SEB가 분리 됨을 확인하였다. CIEX에서 획득한 분획으로 단백질을 크기별로 나누는 GF를 실시하였을 때, Sephacryl S-100 컬럼을 사용하여 정제된 SEB를 획득할 수 있었다. 시료 가 컬럼을 통과할 때 염 농도가 효율적으로 조절될 수 있 도록 하고 SEB를 농축하려 시도한 투석 및 완충액 치환 방법은 전체적인 단백질량 손실을 가져오는 것으로 나타 났다. 본 연구에서 개발된 독소 분리 및 정제 방법은 향 후 다른 장독소 분리에도 활용할 수 있으며, 식중독 신속 진단법 개발 및 항체 생산 발전에 효율을 높일 수 있을 것으로 기대된다.

Salivary proteins include numerous functional proteins which play important roles not only for the food-intake but also for the protective and defensive mechanisms. In the present study the compositions of salivary proteins were analyzed by different methods, including electrophoresis and high performance liquid chromatography (HPLC). In hydrophobic protein HPLC analysis the parotid saliva gradually produced macromolecular complexes when agitated in refrigerator until 30 minutes. These salivary protein complexes were digested by neuraminidase, and then migrated more rapidly in native tris glycine gel than the control. Therefore, it was assumed that the glycosylated proteins of parotid saliva became gradually aggregated to form salivary protein complexes similar to those of whole saliva. The salivary protein complexes were easily degenerated in different experimental buffers, i.e., SDS buffer, tris glycine buffer, methanol, etc., and resulted non-specific patterns in electrophoresis and HPLC. Therefore, it was presumed that the salivary protein complexes was made by the hydrophobic interaction as well as electrostatic attraction between salivary proteins. These data indicated that to know the real pattern of salivary protein complexes in vivo the whole saliva should be analyzed by HPLC using non-adhering column with isoelectric buffer. Consequently, the whole saliva was analyzed by HPLC using reverse phase SuperoseTM column with 20 mM potassium phosphate buffer, and two prominent peaks of salivary protein complexes were consistently found in every people. These salivary protein complex peaks were relatively stable up to 6 hours after saliva collection when the whole saliva was kept in refrigerator during experiment. Therefore, it is suggested that the salivary protein complex patterns are characteristic macromolecular structures of whole saliva, which are also applicable as a diagnostic point in different saliva-associated diseases

황백(Cortex Phellodendri: CP)은 황벽나무(Phellodendron amurense)의 건조된 수피로부터 얻어진다. 이 수피는 한국의 전통 한약제로서 설사, 황달, 무릎과 발의 통증, 요도관 및 피부 감염증에 폭넓게 사용되어 왔다. 이들 기능성성분의 분리 및 정제는 박층 크로마토크래피, 컬럼 액체 크로마토크래피 및 HPLC와 같은 여러 분석법들이 동양의 약초연구에 이용되어 왔다. 본 연구는 CP로부터 berberine을 분리하기 위해 향류분배 크로마토크래피법(CPC)으로 효과적으로 수행하였다. 두 용매의 CPC 최적조성은 n-butanol: acetic acid: water(4:1:5 v/v/v)이었다. 이동상의 유속은 1,000 rpm 회전력에서 상승법으로 분당 3 mL 속도로 전개시켰다. CPC에서 분리된 분획분은 prep-HPLC로 정제하였다. 1H-NMR 스펙트럼은 4.10과 4.20 ppm에서 3H(－OCH3), 6.10 ppm에서 2H의 (－OCH2O－) proton signal의 공명이 관찰되었다. 2개의 방향족 proton은 이중결합 패턴을 보였다. H-11과 H-12 doublet은 각각 7.98과 8.11에서 나타났다. 13C-NMR 스펙트럼에서는 C2와 C3의 methylenedioxy group(－OCH2O－), C9과 C10에 methoxy group(－OCH3)이 4개의 치환된 형태로 보였다. 분리․정제된 berberine의 화학구조는 1H, 13C-NMR, ESI-MS 데이터 분석으로 확인하였다.

The objective of this study was to develop a simultaneous method of 8 penicillin antibiotics including amoxicillin, ampicillin, cloxacillin, dicloxacillin, nafcillin, oxacillin, penicillin G and penicillin V in meat using LC-MS/MS. The procedure involves solid phase extraction with HLB cartridge and subsequent analysis by LC-MS/ MS. To optimize MS analytical condition of 8 compounds, each parameter was established by multiple reaction monitoring in positive ion mode. The chromatographic separation was achieved on a C18 column with a mobile phase of 0.05% formic acid and 0.05% formic acid in acetonitrile at a flow rate of 0.2 mL/min for 20 min with a gradient elution. The developed method was validated for specificity, linearity, accuracy and precision in beef, pork and chicken. The recoveries were 71.0~106%, and relative standard deviations (RSD) were 4.0~11.2%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.003~0.008 mg/kg and 0.01~0.03 mg/kg, respectively, that are below maximum residue limit (MRL) of the penicillins. This study also performed survey of residual penicillin antibiotics for 193 samples of beef, pork and chicken collected from 9 cities in Korea. Penicillins were not found in all the samples except a sample of pork which contained cloxacillin (concentration of 0.08 mg/kg) below the MRL (0.3 mg/kg).

A simultaneous determination was developed for 9 aminoglycoside antibiotics (amikacin, apramycin, dihydrostreptomycin, gentamicin, hygromycin B, kanamycin, neomycin, spectinomycin, and streptomycin) in meat by liquid chromatography tandem mass spectrometry (LC-MS/MS). Each parameter was established by multiple reaction monitoring in positive ion mode. The developed method was validated for specificity, linearity, accuracy, and precision based on CODEX validation guideline. Linearity was over 0.98 with calibration curves of the mixed standards. Recovery of 9 aminoglycosides ranged on 60.5~114% for beef, 60.1~112% for pork and 63.8~131% for chicken. The limit of detection (LOD) and limit of quantification (LOQ) were 0.001~0.009 mg/kg and 0.006~ 0.03 mg/kg, respectively in livestock products including beef, pork and chicken. This study also performed survey of residual aminoglycoside antibiotics for 193 samples of beef, pork and chicken collected from 9 cities in Korea. Aminoglycosides were not found in any of the samples.

The molecular mechanisms of the carcinogenesis of oral squamous cell carcinomas (OSCCs) are highly variable and result in different features of tumor progression, i.e., local tissue destruction and metastasis to regional lymph nodes. A case of OSCC arising from proliferative verrucous leukoplakia (PVL) was analyzed for its protein expression profile by immunoprecipitation (IP) – high performance liquid chromatography (IP-HPLC) by using 72 antisera and comparing results with those of KB cells. OSCC arising from PVL showed stronger expressions of proteins associated with cell proliferation (MPM2, PCNA, eiF5A, DHS, DOHH), cell survival (pAKT, MDM2, survivin), matrix proteolysis (elaffin), tumor suppression (p16, p21, PTCH1), the WNT/β-catenin pathway (SHH, WNT1, APC, β-catenin, snail), proinflammation (TNFα), angiogenesis (HIF, CMG2, vWF), and cellular protection (HSP-70, FAK, caveolin) and of oncoproteins (STAT3, 14-3-3, K-RAS, PUMA, PIM1) and growth factors (EGFR, bFGF) than KB cells. On the other hand, KB cells showed stronger expressions of proteins associated with apoptosis (caspase-3, caspase-8, caspase-9, PARP, FAS, FASL, TGase-1, BCL2, BAD, BID, BAK, FLIP), matrix proteolysis (MMP-2, MMP-9), transcription signaling (NFkB, p38, E2F-1, HO-1), and tumor suppression (p53, RB1, PTEN) and of oncoproteins (DMBT1, CEA) and growth factor (TGF-β1, c-erbB2, VEGF) than OSCC arising from PVL. These data indicate the cells of OSCC arising from PVL are more resistant and more robust than KB cells. Furthermore, they suggest the oncogenic signalings of OSCC arising from PVL play important roles in the aggressive growth and rapid tumor metastasis to regional lymph nodes

영지버섯의 유용한 생리활성을 나타내는 물질은 주로 버섯의 자실체로부터 분리ㆍ동정되었 으며, 이로 인하여 현재까지 다양한 영지버섯의 자실체를 대상으로 그 분석이 수행되고 있다. 그러나 최근 버섯의 균사체에서도 다양한 생리활성을 지니는 물질이 분리ㆍ동정되고 있으며, 이에 따라 버섯의 균사체를 대상으로 하는 생화학적 분석방법의 표준화 등이 요구되고 있는 실정이다. 또한 균사체는 자실체와 달리 배양기간이 짧고, 비용이 적게 소요되어 효율적인 분 석이 가능하다. 그러나 버섯의 균사체는 고분자인 다당류의 함량이 자실체보다 많아 역상 (Reverse phase) 컬럼을 주로 이용하는 HPLC (High Performance Liquid Chromatography) 의 화학적 분석이 용이하지 않다. 이에 본 연구에서는 분석이 어려웠던 균사체 세포 내의 대 사물질 프로파일을 확보하기 위해 부탄올을 이용한 용매 계통 분획으로 고분자 다당류를 제 거하고, HPLC 이동상의 선형 구배 (Linear gradient) 조건을 확립하여 저분자 물질들에 대한 분석 결과를 관찰하였다. 본 연구 결과는 향후 버섯 균사체에 대한 대사체 분석 기술의 기반 이 될 것으로 사료된다.

국내 유통 주류 95점에 대하여 에틸카바메이트 잔류실태 조사를 실시하였다. 탁주, 약주, 청주, 과실주를 비롯하여 소주, 브랜디 그리고 에틸카바메이트 잔류가 많이 보고되고 있는 리큐르 등을 대상으로 하였다. 액액분배를 통하여 정제하였으며, GC/MS/MS 분석법을 정립하였다. 카트리지(cartridge)나 농축 과정이 없기 때문에 식품공전에등재되어 있는 GC/MS 분석법보다 분석시간이나 소요비용 면에서 매우 효율적이었다. 정성한계는 1.3 ug/L이었으며, 정량한계는 4.0 ug/L이었다. 탁주, 약주, 청주에 대하여각각 0.63, 7.01, 14.11 ug/L의 평균 잔류량을 나타내 캐나다의 청주 허용기준 200 ug/L나 약주, 청주에서 논의되고있는 허용기준치 200 ug/L와 비교하여 보았을 때 안전한수준인 것으로 나타났다. 복분자주, 포도주에서는 각각 평균 1.66, 2.64 ug/L 검출되어 현재 캐나다, 체코에서 포도주허용기준치로 설정되어 있는 30 ug/L와 비교하여 보았을 때역시 안전한 것으로 나타났다. 과실주 중 매실주의 경우 평균 79.18 ug/L로 본 연구를 통하여 평가된 모든 주종 중 가장 높은 잔류량을 나타내었으나, 외국의 다른 유사 주종에대한 허용기준치 (예로서 캐나다의 경우 400 ug/L 과실 브랜디)와 비교하여 보았을 때 안전한 수준으로 평가할 수있었다. 증류식 소주, 일반증류주, 리큐르도 논의되고 있는기준치와 비교하여 보았을 때 안전한 것으로 조사되었다. 이러한 조사결과는 우리술의 에틸카바메이트 잔류실태가위험한 수준이 아니라는 근거 자료로 활용할 수 있을 것이며, 향후 이러한 유해물질의 지속적인 잔류조사 및 위해평가를 통하여 우리 술 및 전통발효식품의 품질 및 안전성을 확보해 나가야 할 것이다.

본 논문에서는 축산물 중 닭고기 내 마크로라이드계(MLs) 항생물질(spiramycin, josamycin, tilmicosin, 그리고tylosin) 4종을 분석하기 위하여, 액-액 추출 과정을 거쳐서 PDA 검출기가 장착된 액체크로마토그래피를 이용하여MLs를 효율적으로 동시분석하는 방법을 확립하였다. 컬럼은 Unison UK-C18 (150 mm × 3 mm id, 3 μm), 이동상 용매는 0.1% TFA와 0.1% TFA in ACN로 기울기 용리를사용하였으며, 유속은 0.7 mL/min, 그리고, 주입량은 10 μL로 설정하여 분석하였다. 확립된 분석조건으로, 표준검정곡선은 50-1000 μg/kg의 농도범위에서 상관계수가 0.9975이상의 양호한 직선성을 나타내었으며, 회수율은 저, 중,고농도로 첨가하여 분석한 결과, 80.4-99.1%를 나타내었다. 검출한계는 8.8-19.6 μg/kg이었고, 정량한계는 26.6-59.4μg/kg이었으며, 일내(intra-day)와 일간(inter-day) 정밀도(CV%)는 0.9-13.2%, 2.4-13.1%이었다. 따라서, 확립된 분석방법은 축산물 중 닭고기 시료 내 MLs를 효과적으로분석하는데 이용될 수 있을 것으로 사료된다.