The purpose of this study is to determine the possibility of using Crataegi fructus as a natural food source. To accomplish this purpose, the contents of general and biological activities were measured. The contents of carbohydrate, crude protein, crude lipid and ash are 85.6%, 2.4%, 1.9% and 0.4%, respeectively. Further, the calories of Crataegi fructus was 369.1 kcal. The contents of essential and non-essential amino acids were 852.26 ㎎ and 1, 178.29 ㎎, respeectively. The K was the largest mineral followed by Ca, P, Mg, which means Crataegi fructus is an alkali material. Crataegi fructus extracts slightly(17.6～32.8) inhibited α-glucosidase activity. However, there is no inhibitory activity against α-amylase. In terms of proteslytic activity, Crataegi fructus extracts showed a strong activity than pancreatin(used as a positive control). These results indicate that Crataegi fructus can be used as a natural resource for material aiding digestion.

Stick insect has several peculiarities like parthenogenesis, unique external features and dropping oviposition, so it is very hopeful to use for industrial insect. The oviposition was established by only female without fertilization with sperm of male which was not discovered during this study. The oviposition period was 37.3±14.9 days and the number of eggs per female was 41.5±16.5 eggs. The hatchability was 73.3% at 25℃and 66.7% after low temperature treatment(8℃, 60 days). The developmental periods and the length of egg were 100.9±4.2 days and 0.33±0.0cm, respectively, and the adult's life span was 49.7±16.0days. Differently other research which reported five nymph stage, the developmental stages of nymph showed five(62.9%) or six(37.1%) stages. The duration and the length of nymph increased according to the increase of nymph stage. Stick insect preferred the leaf of American locust, White oak, Chestnut, Bush clover in order but could be reared with food of the leaf of all these trees. Stick insects are herbivorus but made an attack each other when encountered in the small space. For sustaining mass rearing of stick insect, it is needed that the development of artificial diet and high density rearing method.

영광 주변해역에서 꽃게의 갑폭조성, 상대성장, 성비와 외포란량을 조사하였다. 조사해역에서 출현한 꽃게는 총 658개체(수컷:305, 암컷:353)로 나타났다. 조사기간 동안 수온과 염분의 변화 양상은 수온은 5.9-27.3℃의 범위를 나타내었으며, 염분 농도의 경우 28.1-31.6 psu의 범위를 각각 나타내었다. 두흉갑폭(CW)과 갑장(CL)의 관계와 두흉갑폭(CW)과 전중량(TW)의 관계식의 경우 암컷의 경우 CL = 1.3073 CW + 25.684(R2=0.791), TW=0.0061CW2.4231(R2=0.801)로 나타났고, 수컷의 경우 CL = 1.3652 CW + 22.495(R2= 0.741), TW=0.0068CW2.400(R2=0.749)로 나타났다. 꽃게의 성비의 경우 수컷과 암컷의 출현비율이 평균 0.86 : 1로 나타났다. 외포란량의 경우 암컷의 크기가 증가함에 따라 포란량은 증가함을 알 수 있었다.

We surveyed natural enemies of Pachynematus itoi Okutani, newly recorded sawfly pest of Larix leptolepis, and investigated their occurrence and life cycle at Cheolwon and Taebaek, Gangwon Province in 2006～2007. Endasys liaoningensis was identified as main natural enemy of P. itoi Okutani. Parasitism of E. liaoningensiswas was 42%. Emergence period of E. liaoningensis was from May to September, and the peak of emergence was from 21th May to 20th June. It was suggested that the occurrence number and period of E. liaoningensis were quite closely relative to those of host, P. itoi.

We have investigated some biological characteristics, such as survival rate and fecundity, of Orius laevigatus and Phytoseiulus persimilis, which have been stored at 6, 8, 10, 12±1℃, RH 70±10%, in dark condition. Overall, an appropriate temperature for cold storage was 10℃ for O. laevigatus and 8℃ for P. persimilis. 70% of adult O. laevigatus could survive for 36 days at 10℃. The O. laevigatus stored at 10℃ for 10~50 days laid 37.1～120.5 eggs. Since fresh (no storage) O. laevigatus laid 224.5 eggs, comparative fecundity of stored adults was 16.5~53.7% of normal fecundity. P. persimilis stored at 8℃ for 7~42 days with (food eggs of Tetranychus urticae) laid 11.9～18.9 eggs. Since fresh P. persimilis laid 26.4 eggs, comparative fecundity of stored adults was 45.1~71.6% of normal fecundity.

For biological control of larva of Cnaphalocrocis medinalis and Naranga aenescens do considerable damage to crops by folding and scraping the leaf tissue of rice, a large number of Bacillus thuringiensis isolates have been obtained from soil samples in Korea and the pesticidal activity was assayed against two insect pest species described above. Among 53 Bt isolates tested in bioassay, 18 and 13 isolates showed over 90% mortality against C. medinalis and N. aenescens, respectively. Some isolates (11 isolates including CAB141) presented dual activity against C. medinalis and N. aenescens. These isolates showed over 96% control effect in pest control in laboratory against larvae of C. medinalis. Also, it was investigated that pupation, pupal length, and adult emergence of larvae exposed to Bt suspension decreased. Especially, the pupal length of C. medinalis after being fed corn seedling leaves treated Bt suspension for 10 days, were much smaller than that of control.

Biological Characteristics of Lycorma delicatula and insecticidal activity of some insecticides against nymphs of L. delicatula was investigated. Nymph of L. delicatula had 4 instars, and color of body was black. There were white spots on the body of 1st-3rd nymph. Upper body became red at 4th nymph. Adult forewings were brownish, and had black spots. Color of hind wing were red. The egg mass was covered with a yellowish brown secretion. The adult of L. delicatula emerged once a year. Among test insecticides, deltamethrin 1% EC and fenitrothion 50% EC showed very quick and strong insecticidal activity against the 2nd-3rd nymphs of L. delicatula. Imidacloprid 4% SL and clothianidin 8% SC showed 100% insecticidal activity at 24h after treatment. Thiacoprid 10% SC revealed the weakest insecticidal activity among the insecticides tested.

With the comparison of native Orius strigicollis (Poppius) to European O. laevigatus (Fieber), we investigated biological characteristics such as developmental period, fecundity, life span, predation ability, and augmentation after release. Experiments were carried out at five temperature condition, 15℃, 20℃, 25℃, 30℃, and 35℃. In the case of O. strigicollis, egg period was 3.1~14.9 days (hatchability: 46.7~88.2%), and developmental period of nymphs was 9.4~42.8 days (survival rate: 2.4~96.5%). Fecundity at each temperature was 28.1, 107.9, 123.6, 127.3, and 18.1, respectively. Life span of adults was 63.4, 40.1, 22.1, 18.3, and 11.0 days, respectively. In the case of O. laevigatus, egg period was 3.1~13.5 days (hatchability: 65.0~89.9%), and developmental period of nymphs was 9.3~42.0 (survival rate: 4.3~80.6%) days. Fecundity at each temperature was 101.8, 218.6, 224.5, 219.5, and 15.7. Life span of adults was 70.6, 66.4, 32.6, 34.5, and 7.3 days, respectively. In long-day condition (16L:8D), fecundity of O. strigicollis and O. laevigatus was 105.8 (18℃)~142.4 (25℃) and 109.5 (18℃)~191.5 (25℃), respectively. In short-day condition (10L:14D), fecundity of them was 1.0 (18℃)~31.8 (25℃) and 63.0 (18℃)~198.8 (25℃), respectively. Daily prey consumption of second instar Frankliniella occidentalis was 14.3 and 10.9, respectively. In greenhouse, density of O. strigicollis begins to increase from mid May and peaked about early July (1.8 individual/flower), while that of O. laevigatus begins to increase from mid March and peaked about early June (6.6 individual/flower).

This study was performed to investigate the immature development period, fecundity, emergence rate and sex ratio of Sclerodermus harmandi against different host insects, Monochamus alternatus, M. saltuarius and Psacothea hilaris. Full grown larvae and pupae of host insects were provided with foods. The mean larval period of S. harmandi female was 29.2±0.93 and 25.1±0.47 days in larvae and pupae of M. alternatus, 27.1±0.41 and 26.0±0.69 days in M. saltuarius, and 26.3±0.38 and 31.2±0.24 days in P. hilaris, respectively. S. hilaris adults were emerged at 12.9±0.2 days in female and 11.9±0.2 days in male after pupation when hosted M. alternatus pupa. Development period in male showed shorter one day than in female. Success rate of oviposition against different hosts was higher as 98.6 and 97.5% on full grown larva and pupa of M. saltuarius. Emergence rate was higher as 90.1 and 87.3% on M. saltuarius larvae and pupae. Sex ratio of emerged S. harmandi adults was approximately 10:1 (Female : Male), females showed higher emergence rate than males. The period until first oviposition after emergence in S. harmandi female was the shortest in 4.6±0.1 days on M. saltuarius pupa. When three females of S. harmandi were inoculated on M. saltuarius larva, the number of laid eggs was the highest 62.7±2.5 per female.

The characteristic of implant s llrface is the most i mportant factor in osseointegra t ion procesR , Prol i fera tion and rli ffel' entiat ion of osteoblast, a nd local factors concerning bone forma tion a l'e inflllenced by sUl' face cha racteri s tic and it al so con t l'ols bone reactlOn The purpose of this reseal'ch was to s tlldy a boll t ini t ial adhesion. prolife ration and acti vation of osteo blast to titanium surface treated wi th mac hined‘ hydroxyapat ite coating, resorba ble blast materi al blasting and a n odi zing method To attach and culture osteobl ast‘ titanium cylinder block wi th 5mm in di ameter and 5mm in height was mad e , After t l'eating the titanium surface of each bl ock with machined, hydroxyapatite coating, I'esorhable blast ma teri al blasting and anodi zed coating, i.mpllrities wel'e I'emoved and stenllzed The number of cells attached from cultul'ed osteobl ast of I'espective expel’imental groups were measured at 1, 4, 7. and 14day, Al ka line phosphatase, calcium. and inorganic phosphate concen tra ti on 0 1' cultured solution was measul'ed, Anodi zing group showed the hi ghest. and RBM treated grollp was foll owed, Machined grollp was the worst ra te of cell a ttachment and pl 미 ife ra ti o n activity, RBM t l'eated grollp s howed the high est in creasi ng on theil‘ alkaline phosphatase activi ty of 1 and " days in cultllred osteobJast to compnrc wi th othcr groups. Thc rc was no significant differe nce among other grollps , stati stically, RBM trea ted grollp showed the hi ghest rate 0 1' increa s ing on the ca lcillm apposition of 1 a nd 4 days in cllltured osteobl ast to compa re wi th other groups , The re was no s ignificant differ ence among other groups ‘ statistica lly, RBM t reated g roup showed the highest rate of inorganic phospha te apposi t ion to compare with other groups , The re was no s ignifi cant diffe rence among other groups‘ s tati stically, It suggested tha t surface modi fï cation of titani um would be profoundly effected on the attachment. proliferation and activation 0 1' osteoblast in init ial stage osseolll tegrat lOn

Methic illin - resistant StaphyJococcus aureus(MRSA) is not only a common cause of nosocomial infections worldwide but also leading to seri ous illnesses with high rates of mortali ty , This study was performed to determine resistance patterns and molecul ar biological ca racteri slics on MRSA isolated from clinical specimen, The purpose is to provide valua ble in formation related to t he purs uit 0 (' in('ec tion trace and infe<:tion control The test of susceptibility to 17 kinds antibiotics showed that these bacterias were susceptible to glycope ptides s uch as vancomycin, teicoplanin by 100%, rifarnpin by 65, 8%, They we re a lso 100% resistance to peni cillin , cefotaxim, gentamycin and aminoglycoside drugs , and showed 68, 56% multidrug res is ta nce to a bove 13 a ntibi oti cs incl uding peni cillin, 1n the analysis of MRSA using PCR, mecA was detected in every MRSA at 35 s t ra i ns a nd l'emA in 94 1%, ln a test , to de tect toxic genes, six genes, such as sea, seb(8, 6% res pectively)‘ sec(51, 4%) ‘ seg, se i(65 , 7% res pectively) and seh(2 , 9%) were found Finally , in resisrance chract eristics, toxic genes we re isola ted c1 ifferently acco rcling to specimen but there was no c1ifference res istance pattern ancl toxic genes The c1ata from t his s t ucly contribule to a more prec ise knowledge about the resistance pa ttern of MRSA in a clinical sett ing in k orea ancl regarcl exa mi na ti on of resis ta nce pattern accorcling to molecular biological methocl are expectecl to provide bas ic informa ti on on MRSA di ssemination and infection contro

This study was carried out to clarify the biologies and morphological characteristics of Rhynchaenus sanguinipes. Also some chemicals were tested to screen the effective insecticide for the control of the species. Up to date, Zelkova serrata has been known as host plant of Rhynchaenus sanguinipes, which shows serious damage in this country. In the present study, Ulmus pumila was first found as host plant in this study. Body lengths of larvae, pupa and adult were 4.53±0.30 ㎜, 3.30±0.42 ㎜ and 2.96±0.12 ㎜, respectively. The overwintered adult of the species emerged on early April to late April, and adult of next generation emerged on early May to late May. Pupal periods were 10, 7.2, 5.1 and 4 days on 16, 20, 24 and 28℃, respectively. The lower developmental threshold temperature was 5.8℃. Four braconid parasitoids were found as natural enemies, which emerged mainly on late April to early June. Insecticidal activities with treatments of fenitrothion 50% EC, indoxacarb 30% WG, ethofenprox 20% EC and thiacloprid 10% SC was investigated against adult of R. sanguinpes, and they showed ＞90% mortality.

본 연구에서는 산성전리수의 일반적인 생물학적 특성을 간략히 살펴보았다. 직선형 DNA를 산성전리수에서 4˚C와 25˚C에서 약 10분간 반응시킨 결과 각각 40%와 50%의 DNA가 분해되었다. 그러나 산성전리수를 사용한 고온에서의 DNA 증폭반응 실험에서 DNA 분해없이 정상적으로 DNA증폭반응이 일어났다. 산성전리수가 단백질의 안정도에 미치는 영향을 살펴본 결과 증류수에서는 총 7일 동안의 반응시간동안 단백질의 분해가

진딧물의 포식자인 진디혹파리(Aphidoleres aphidimyra)의 온도와 먹이에 따른 발육기간, 산란력, 부화율, 우화율, 포식능력, 번데기의 저온저장, 살충제에 대한 감수성 등의 생물학적 특징을 조사하였다. 복숭아혹진딧물을 먹이로 공급하였을 경우, 알에서 성충까지의 평균 발육기간은 15에서 40일, 3에서 12일이 소요되었다. 또한 목화진딧물을 먹이로 공급하는 경우에는 이보다 짧아 각각 37일과 11일이 소요되었다. 번데기를 저장하는 경우에는 5에서는 1주일, 1에서는 2주일 동안 보관하여도 90%이상의 우화율을 나타내었으나, 그 이상 저장하는 경우베는 우화율이 점차 떨어지는 경향을 나타내었다. 성충은 우화 2-3일 후에 교미를 하고 알을 낳기 시작하여 평균 10일동안 약 200여개의 알을 낳는다. 대부분의 성충은 오후 6시에서 8시 사이에 우화하고 주로 야간에 활동하였다.

애꽃노린재류(Orius spp.)나 알기생봉류(Trichogramma spp.)와 같은 천적곤충의 대량사육을 위한 대체먹이로서, 국내에서 채집이 가능한 저장곡물해충인 줄알락명나방(Cadra cauteila)의 생물적 특성을 조사하고 대량사육 방법을 개발하였다. 에서 줄알락명나방의 발육기간은 알, 유충, 번데기가 각각 4.2일, 29.8일, 8.3일이었으며, 성충의 수명은 암컷이 5.8일, 수컷이 4.8일이었다. 총산란수는 20, 25, 3에서 각각 128.9개, 207.9개, 139.9개였다. 실험에 사용된 줄알락명나방의 사육사료 모두 발육기간, 우화율, 성충무게 면에서 큰 차이가 없었으나, 대량사육을 위해서는 쌀겨 50%와 병아리사료 50%를 혼합한 사육사료가 비용 면에서 보다 경제적이었다. 줄알락명나방알의 저장조건으로 9에서 7일간 저장이 부화율 82.0%로 4보다 우수하였다. 유충사육케이지 (16249cm)에서 알의 초기접종밀도를 1,000개, 3,000개, 5.000개로 할 때, 우화율은 각각 62.1, 42.4, 29.4%, 성충무게는 각각 9.1, 77, 6.8 mg이었다. 줄알락명나방의 대량사육체계를 \circled1 사료 배합 \circled2알접종 및 유충사육 \circled3번데기 이동 \circled4성충 우화 \circled5알 수확 단계로 구성하고 각각의 과정을 상세히 설명하였다.

Tetrastichus sp.는 미국흰불나방의 번데기 기생벌로서, 1개의 숙주에 여러마리의 자손을 번식하는 집단 기생벌이며, 일년에 여러 세대 발생한다. 작잠, 신생아용 분유, 난황, 로얄젤리 등을 주요성분으로 기생벌의 인공먹이를 조성하였으며, polypropylene필름으로 번데기형태를 만들어 인공숙주로 사용하였다. 인공숙주에서 발육한 기생벌은 자연숙주에서 자란 기생벌보다는 크기가 작았으나, 계대 사육으로 번식 가능한 자손을 생산하였다. 인공숙주에서 발육한 in vitro 2세대의 생물학적 특성은 in vitro 1세대보다 훨씬 개선 었다. 평균 자손 수, 산란기간, 수명 등은 in vitro 에서 45.7,7.8, 13.8일 이었고 암컷 편중의 성비 76.9%를 나타내었다. 이러한 결과는 Tetrastichus sp.가 in vitro 대량사육에 적합한 기생벌임을 보여준다.