잎응애류의 포식성 천적인 민깨알반날개의 실내사육에 필요한 몇 가지 생태적 특성 즉 산란 습성, 성충우화율, 저장온도 그리고 귤응애의 밀도억제효과를 시험하였다. 민깨알반날개 암컷성충은 유자잎 뒷면에 를 산란하였으며, 그중 를 귤응애의 탈피각이나 배설물로 알 표면을 피복하였다. 과수원 내 밭토양에서는 민깨알반날개의 우화율이 였는데, 밭토양 대신 원예용상토를 사용했을 때 로 향상되었다. 대부분의 유충은 지면으로부터 2 cm이내 깊이에서 번데기가 되었다. 성충 저장에 가장 적합한 온도는 발육영점온도인 이며, 저장 10, 20, 30일 후에 생존율은 각각 96.7, 73.3, 였다. 유자에 발생한 귤응애 방제를 위한 효율적인 방사비율은 150:1 미만으로 방사하였을 때 가장 효율적이었다.

PE box, wrap, 그리고 25um와 50um 두께의 LDPE(low density polyethylene) 필름으로 포장한 치콘을 저장온도 1℃ 와 10℃에서 광조건과 암조건으로 나누어 저장성을 비교하였다. 치콘의 저장 중 생체중 감소는 2% 수준에서 외관상 품질 저하가 발생하였는데, 밀폐되지 않는 PE box의 경우 1℃에서는 2%, 10℃에서는 3%의 생체중 감소를 보였다. 이에 반해 무공필름이었던 wrap, 그리고 25um와 50um 두께의 LDPE(low density polyethylene) 필름에서는 1℃와 10℃ 모두에서 1%미만의 감소를 나타내었다. 포장재내 공기 조성은 이산화탄소의 경우 1℃의 50um LDPE 필름과 10"C에서는 25um LDPE 필름 처리구가 3~4% 수준을 보였다. 에틸렌은 가장 높은 함량을 보인 50um LDPE 필름에서 온도별로 1℃에서 0.3ppm, 10℃에서는 0.5ppm으로 낮은 수준을 보였다. 저장중 greening은 암처리에서는 나타나지 않았으나 광처리의 경우는 10℃에서는 저장 3일만에 1℃의 경우도 6일만에 판매하기 곤란한 상태까지 진전되었는데, 1℃의 경우 포장재 종류별로 포장재가 두꺼울수록 greening의 진행이 지연되는 경향을 보였다. Greening을 수치화 할 수 있는 엽록소 함량은 역시 저장온도가 낮은 1℃가 10℃보다 낮았고, 역시 이산화탄소 농도가 가장 높았던 50um LDPE필름에서 가장 낮은 함량을 보였는데 포장재 내부의 이산화탄소 함량과 총 엽록소 함량과의 상관관계를 조사한 결과 상관계수(r)가 1℃에서 0.926 10℃에서는 0.997로 고도의 상관이 있음을 알 수 있었다. Greening을 제외한 외관상 품질은 저온인 1℃에서 높게 유지되었고 포장재별로는 1℃에서는 50um LDPE 필름이 10℃에서는 25um LDPE필름에서 가장 높은 점수를 나타내었다. 비타민 C 함량도 저온에서 높게 유지되었으며 필름종류별로는 25um와 50um LDPE 필름에서 가장 높았다. 이상의 결과로 보아 치콘의 저장 및 유통시 1℃에서는 50um LDPE 필름이 10℃에서는 25um LDPE 필름이 포장재로 적합한 것으로 사료된다. 또한 약간의 빛으로 greening이 급격히 진행되므로 판매과정에서 암조건을 유지하는 것이 필요하리라 생각된다.

This study was carried out to determine the storage, cryotolerance, heat and drying resistance when the L. acidophilus growing at optimal temperature was changed growth temperature and to apply it to probiotics production so as to help intensify the viability of lactic acid bacteria in the products. L. acidophilus CT 01 was changed temperature at 22℃ and 28℃ after incubated at 37℃ for 18hours. The viable cell number increased to 4.9×108 and 5.0×108 cfu/mL, respectively in the changed temperature groups, but decreased in the control, rapidly down to 7.4×105 cfu/mL in 36 hours. L. acidophilus CT 01 was stored at 4℃ for 12 days after changed temperature at 22℃ and 28℃ for 48hours, changed temperature groups had a survival rate of 112% and 94.43%, higher than that of control (p<0.05). When stored at room temperature, survival rate in the control was 10.88%, very low, but changed temperature groups had very high survival rate, 74.43% and 85.62% (p<0.05). The cryotolerance of L. acidophilus CT 01 appeared higher than changed temperature groups was observed at 37℃ for 66hours. L. acidophilus CT 01 was heat treated at 60℃ for 15 minutes and 30 minutes to examine heat resistance after changed temperature, the heat resistance of L. acidophilus CT 01 appeared higher than control was observed changed temperature groups. L. acidophilus CT 01 was inoculated by 30% to the carrier after changed temperature, and dried at 50℃ for 12 hours. The highest survival rate of L. acidophilus CT 01 was observed changed temperature groups.

Salmon mince에 우유단백질 농축물인 MPC를 첨가해서 저장온도별 경과일수에 따라 salmon mince의 기름을 추출해서 POV를 측정하여 산화 안정성을 살펴 보았다. Salmon mince를 4℃에서 21일간. -l8℃에서 2()일간, 전자오븐에서 조리한 후 -l8℃에서 20일간 저장하여 저장온도별 경과일수에 따라 POV를 측정한 결과 salmon mince에 4% MPC 첨가한 것이 첨가하지 않은 대조군에 비해 산화 안정성이 증대되었다. 조리직후 4% MPC 첨가군이 대조군보다 POV가 낮은 현상을 나타냈는데, 이는 가열에 의해 MPC중의 항산화성을 지닌 물질의 노출에 의한 것으로 생각된다 또한 산화 안정성을 측정하기 위한 관능평가에서도 4% MPC 첨가군이 대조군에 비해 양호하였다. 이와 같은 결과는 MPC가 salmon mince의 단백질 안정제로서 산화 안정성 증가에 유용하게 사용할 수 있는 것으로 나타낸 것이다.

사과주스 농축품의 저장온도에 따른 저장 중 품질변화를 살펴보기 위하여 45 Bx 혼탁과 72 Bx 청징 사과주스 농축품을4℃, -3℃ 및 -l5℃에서 16주 동안 저장하면서 미생물, pH와 산도, 색, alcohol soluble color 및 비타민C 함량의 변화를 살펴보았으며. 이들 이화학적 특성간의 상관관계를 살펴보았다. 혼탁사과 주스 농축품을 4℃에서 저장한 경우에만 저장기간이 길어짐에 따라 호기성 일반세균 및 효모류의 수가 크게 증가하였으나 -3℃와 -l5℃에서 저장한 경우와 청징 농축품의 모든 저장온도에서는 미생물이 검출되지 않았다. 적정산도 역시 4℃에 저장한 혼탁사과주스 농축품에서 다소 증가하였으나pH의 경우에는 3.6∼3.8로 큰 변화가 없었다. 색의 경우 혼탁과 청징 농축품 모두 저장기간이 길어지거나 저장온도가 높을 경우에 a값이 크게 증가하였으며 ASC 역시 이와 유사한 경향이었다. 비타민 C의 함량은 저장기간이 길어짐에 따라 감소하였으며 저장온도가 높을수록 감소경향이 크게 나타났다. 적색도를 나타내는 a값과 ASC간에는 0.957∼0.967 의 높은 상관계수값을 나타내었으며 비타민 함량도 역시 -0.864∼0.936의 상관계수 값을 나타내었다.

본 실험은 Japanese mint의 생체저장에 있어 저장온도와 필름종류의 영향을 비교하여 적정 저장조건을 제시하고자 실시하였다. 생체중 감소로 볼 때 Japanese mint는 다소 두꺼운 40μm 이상의 필름이 효과적이었다. 저장 중 필름내 가스농도는 필름이 두꺼운 CE 80(ceramic 80μm 필름)이 CE 40(ceramic 80μm 필름)보다 높았으며 저장온도별로는 3℃이하에서는 저온장해가 발생하여 온도별 차이가 없거나 오히려 1℃에서 가장 높은 가스농도를 보였다. 저장 중 엽록소 함량감소는 5%이상의 수분손실과 0.5ppm이상의 에틸렌에 의해 촉진되었으나, 4%이상의 이산화탄소농도에서는 그 감소는 억제되었다. 저장수명은 3℃저장에서 30일로 다른 저장온도의 두배였다. 가장 저장수명이 길었던 3℃에서 저장 최종일에 외관상품질은 CE 40에서 가장 좋았으며 저온장해 정도를 나타내는 이온용출량도 가장 낮게 나타났다. 이상의 결과로 볼 때 Japanese runt 저장시 적정 저장온도는 3℃이며, MA저장시 포장재로는 ceramic 40μm 필름이 적절하였다.

Effect of storage temperature, washing, and cooking on postharvest-treated pesticide residues in polished rice was investigated. After being traeted with each 500 mg/kg of captan, carbaryl, phenthoate, fenthion, fenitrothion, chlorpyriphos-methyl, pirimiphos-methyl, the polished rice was stored for 8 weeks at 4 and 30℃, respectively. The penetation rate of carbaryl was the highest as 27.5% and the others, 13 to 18%. The half lives of pesticide residues were estimated as 30 to 230 weeks at 4℃, but 1 to 12 weeks at 30℃. The residues were reduced faster at 30℃ than at 4℃. The half lives of pesticide residues by water washing were estimated as 0.7 to 4.6 trials and the residues were removed with the washing trials. The residues of captan and carbaryl in cooked rice were removed 100 and 98%, respectively, comparing to initial residues concentration in treated rice but those of other 5 pesticides were removed 80%.

There were few data for the distribution of the indicator organisms in the commercial plant foods, and for the normal flora and for the foodborne agents within the country. First of all it must be investigated the distribution of the indicator organisms. And also it is very important to prepare the sanitation criteria for the plant foods through the microbiological e×amination and the investigation of tendency to change of the indicator organisms according to the storage temperature and period. The average number of total viable counts for grains was 2.9 × 10^5/g, psychrophilic bacteria 2.9 × 10^6/g, heterotrophic bacteria 3.1 × 10³/g, heat-resistant bacteria 2.1 × 10³/g Pseudomonas aeruginosa 23/g. That for beans was 6.3 × 10²/g, psychrophile 34/g, heterotroph 1.7 × 10²/g. That for sesames was 1.4 × 10^5/g, coliform 350/g, psychrophile 7.4 × 10⁴/g, heterotroph 5.8 × 10⁴/g, Pseud. aeruginosa 2.3 × 10³/g. heat-resistant bacteria 150/g. That for potatoes was 2.0 × 10^7/g, coliform 5.0 × 10⁴/g, psychrophile 1.8 × 10^7/g, heterotroph 1.4 × 10^7/g, heatresistant bacteria 3.3 × 10¹/g, Staphylococcus 2.7 × 10^5/g, fecal streptococcus 4.5 × 10³/g, Pseud. aeruginosa 7.0 × 10³/g. That for mushrooms was 1.2 × 10^8/g, psychrophile 9.4 × 10^7/g, heterotroph 1.0 × 10^9/g, heat-resistant bacteria 1.6 × 10^5/g, Pseud. aeruginosa 1.3 × 10³/g. That for vegetables was 5.9 × 10^(11)/g, coliform 1.8 × 10^6/g, psychrophile 1.1 × 10²/g, heterotroph 8.4 × 10^(11)/g, heatresistant bacteria 7.6 × 10^6/g, Staphylococcus 1.1 × 10^7/g, fecal streptococcus l.1 × 10⁴/g, Pseud. aeruginosa 5.2 × 10⁴/g. That for nuts 3.9 × 10⁴/g, coliform 3.9 × 10³/g, psychrophile 4.0 × 10^8/g, heterotroph 3.2 × 10^8/g, heat-resistant bacteria 400/g. In commercial grains and beans, SPC, psychrophile, heterotroph and heat-resistant bacteria stored at 10℃, 20℃, 30℃ were constant. Staphylococcus, coliform, Pseud. aeruginosa were decreased a little in grains, but were not detected in beans. In mushrooms, all indicator organisms were increased as time goes on and were increased rapidly at 20. In sesames, coliform was not detected at all temperature. psychrophile was increased for 7 days, the otners were constant. In potatoes, SPC, psychrophile, heat-resistant bacteria, heterotroph had a tendency to increase and the others were constant. In vegetables, indicator organisms were had a tendency to increase, psychrophile, heterotroph were rapidly increased after 7 days. In nuts, SPC, coliform, psychrophile, heterotroph, heat-resistant bacteria, Pseud. aeruginosa were constant, staphylococcus and fecal streptococcus were not detected.

Phytic acid, making up 1-5% of the composition of many plant seeds and cereals, is known to form iron-chelates and inhibit lipid peroxidation. Thiobarbituric acid reactive substances (TBARS), as an indication of lipid peroxidation, were measured in beef round, chicken breast, pork loin, and halibut muscle after the meats were stored for 0, 1, 3, 5, and 7 days at various temperatures [frozen (-20℃), refrigerator (4℃), and room temperature (25℃)]. Phytic acid effectively inhibited lipid peroxidation in beef round, chicken breast, halibut, and pork loin muscle (p$lt;0.05). The inhibitory effect of phytic acid was dependent on concentration, storage time, and temperature. At frozen temperature, the inhibitory effect of phytic acid was minimal, whereas at room temperature, the inhibitory effect of phytic acid was maximal, probably due to the variation of the control TBARS values. At the concentration of 10 mM, phytic acid completely inhibited lipid peroxidation in all the muscle foods by maintaining TBARS values close to the level of the controls, regardless of storage time or temperature (p$lt;0.05). The rate of lipid peroxidation was the highest in beef round muscle, although they had a close TBARS value at 0 day. Addition of phytic acid to lipid-containing foods such as meats, fish meal pastes, and canned seafoods may prevent lipid peroxidation, resulting in improvement of the sensory quality of many foods and prolonged shelf-life.

The average number of total viable counts for the commercial pork tested was 19/g, coliform 1.8/g, psychrophilic bacteria 15/g, heterotrophic bacteria 12/g, fecal streptococcus 6.2/100 g, Pseudomonas aeruginosa 13/100 g and none of heat-resistant bacteria and Staphylococcus was detected. That for the commercial beef tested was 130/g, coliform 5.2/g, psychrophile 140/g, heterotroph 28/g, Staphylococcus 1.2/g, fecal streptococcus 9.5/100 g, Pseud. aeruginosa 1.9/100 g and heat-resistant bacteria was not detected. That for the commercial chicken tested was 8800/g, coliform 53/g, psychrophile 4600/g, heterotroph 4700/g, fecal streptococcus 9.9/100 g, Pseud. aeruginosa 2.5/100 g. That for milk was 4700/ml, psychrophile 120/ml, heterotroph 420/ml and the others were not detected. That for the commercial cheese was 3.2/g, psychrophile 2.3/g, heterotroph 1.6/g, Staphylococcus 1/g, fecal streptococcus 9.1/g. That for fermented milk was 10^7/ml, heatresistant bacteria 10^6/ml, fecal streptococcus 2400/100 ml, lactobacillus 3.2 × 10^(15)/ml, in accordance with lactic acid bacteria and the others were not detected. There was not detected any indicator organisms from ham, sausage, butter, eggs and quails in the commercial fooods tested. SPC, coliform, psychrophile and heterotroph in commercial meats stored at 10℃ were increased rapidly as time goes on but heat-resistant bacteria, staphylococcus, fecal streptococcus and Pseud. aeruginosa were constant. At 20℃, SPC, coliform, psychrophile, heterotroph and fecal streptococcus were the highest at 7 days and heat-resistant bacteria, staphylococcus and Pseud. aeruginosa were increased a little. At 30℃, all indicators were increased rapidly for 3 and 7 days and then decreased rapidly. All indicator organisms were increased at the level of 10/g for 14 days in meat products stored at 10℃, but SPC, psychrophile and heterotroph in meat products stored at 20℃ were increased at the level of 10^5/g. It showed that the indicators in meat products stored at 30℃ had a tendency to increase at the level of l0₂/g relative to those stored at 20℃. SPC, psychrophile and heterotroph in milk stored at 10 increased up to the level of 10₄/ml, but coliform, staphylococcus, fecal streptococcus and Pseud. aeruginosa were not detected. As stored at 20℃ and 30℃, they were increased rapidly for 1 or 3 days and then constant for a long time.

쇠고기의 사태, 갈비 및 등심부위를 8℃에 저장하면서 육질의 변화를 검토하기 위해 전단력가, 근원섬유의 소편화, actomyosin의 추출성 및 ATPase 활성을 측정하였다. 사태 및 등심부위의 전단력가는 저장 6일째 현저하게 낮아졌고, 갈비부위는 저장기간 동안 현저한 차이가 없었다. 저장초기 부위별 전단력가는 갈비, 등심 및 사대부위의 순으로 현저한 차이가 있었으나 저장기간이 경과하면서 갈비 및 등심부위는 비슷하였다. 근원 섬유 소편화도의 경우 사태부위는 저장 6일째 현저한 증가를 보였지만 갈비와 등심부위는 저장기간동안 계속 증가하였다. 근원섬유의 소편화율도 소편화도와 비슷한 양상이었으며, 갈비와 등심부위는 사태부위와 다른 변화를 보였다. Actomyosin의 추출성은 저장초기 갈비, 등심 및 사태부위의 순으로 높게 나타났으며 3일째 모든 부위가 증가하다가 등심부위는 저장 6일째 감소하였다. Actomyosin의 Ng^2+-ATPase 활성의 경우, 사태부위는 저장 3일째 증가하다가 6일째 저장 0일의 수준으로 회복되었고, 갈비 및 등심부위는 비슷한 변화를 나타내었지만 0일째는 등심부위가 갈비부위보다 높게 나타났다. 그리고 사태부위의 Ca^2+-ATPase 활성도 저장 3일째 증가하다가 6일째 감소하였으며, 갈비부위는 저장중 변화가 없었고, 등심부위는 저장기간동안 완만하게 감소하였다.

This study was carried out to investigate the microbiological and chemical properties of natural water during storage. The water samples were taken at the time of purchase and the opened bottles and the unopened bottles stored at the temperature of 4, 18, and 30. The bacterial content normally rose to 2.06 × 10² CFU/ml for the unopened bottles and 2.91 × 10² CFU/ml for the opened bottles after 2 weeks of storage, and 1.21 × 10^7 CFU/×ml and 2.64 × 10^7, respectively, after 24 weeks of storage. The number of viable cells of bacteria peaked more rapidly at the storage temperature of 30℃ than 18℃. But the total samples were found to be negative for coliforms test during the study period. The average range of pH value was from 7.39 to 7.76. The results showed that the nitrates and chlorides satisfied the Korea Drinking Water Quality Standards during the storage period of 24 weeks. However, the undesirable changes of the taste and odor were detected within 2 weeks and 3 weeks, respectively.

The quality changes of hams and sausages in refrigerated storage were investigated. Seven types of hams and six types of sausages produced in Korea were collected from markets and stored at 10℃ according to the Food Code of Korea, and then chemical, microbiological, textural and sensory characteristics were evaluated at 30, 40 and 50 days. The proximal analysis showed considerable variation in fat with less variation in moisture and protein. The pH values of hams and sausages slightly changed with no consistent difference. Water activity values of all samples except one type of sausage were consistent over time in refrigerated storage. No purge losses of hams were observed except two types of samples in the intial stage. Several types of sausages showed purge losses with no consistency and one type of sausage showed consistent purge losses (0.44-11.29%) during the storage. The volatile basic nitrogen (VBN) content of hams and sausages was still within 20 mg% (Standard and Specification of Korea) on the 50th day, although the VBN of all samples significantly increased over time (p$lt;0.05). The thiobarbituric acid (TBA) values of hams and sausages were well below 1.0 during the storage, although the TBA values of all samples significantly increased (p$lt;0.05). However it was noticed that one type of ham and two types of sausages showed TBA values of 0.945, 0.928 and 0.978, respectively. All the standard plate counts (SPCs) of hams during the storage period showed a level below 30 CFU/g except one type of sample with 10³ CFU/g at 50 days. All the SPCs of sausages showed a level below 50 CFU/g except one type of sample with 10² CFU/g and two types of samples with 10⁴ CFU/g on the 50th day. Coliform groups were not counted in all samples during the storage. Instrumental texture analysis showed that springiness, cohesiveness, chewiness, gumminess and adhesiveness slightly changed with no consistent difference. The hardness of the hams showed a decreasing tendency, and the hardness of the sausages an increasing tendency with no significant difference. Sensory evaluation for color, flavor, taste, texture and overall acceptability showed decreasing tendencies over time (p$lt;0.05). The scores of the five sensory characteristics maintained the medium level of quality at the termination of the experiment. Although the results indicated that the ham and sausage samples were acceptable after the 50 days' storage at 10℃, the shelf-life of the samples should be determined considering the rapid growth of bacteria and the high TBA values at 50 days.

In order to investigate bacterial sanitary condition of fish and shellfish, we examined the normal flora in the 25 species of commercial fish and shellfish, and also proportional change of normal flora by storage period and temperature. Isolated 334 were isolated in the normal fish and shellfish and predominant genera were Pseudomonas (25.2%), Staphylococcus (10%), Acinetobacter (7.2%), Vibrio (6.9%), Micrococcus (5.4%), Aeromonas (5.2%), and Enterobacter (5.2%). In accordance with storage period and temperature, Pseudomonas grew on high ratio at 10℃ steadily, but Proteus had increased proportionally at 20℃ and 30℃. Additionally, Citrobacter, Moganella, and Pasteuralla had increasd, while Achromobacter, Acinetobacter, Yeast, and Micrococcus had decreased by period.

This paper intends to investigate commercial fish and shellfish 25 species (fish 8 species, shellfish 7 species, crustacean 3 species, molusc 4 species and echinodermata) for the distribution of sanitary indicator organisms (total viable counts, coliforms, staphylococci, vibrios, and enterococci) and diatributional change of indicator organisms according to storage temperature and period. The logarithmic mean of total viable counts for total commercial fish and shellfish 25 species was 5.41±0.26 CFU/g, and in accordance with fish and shellfishes, crustacean 6.76±0.67 CFU/g, shellfish 5.67±0.56 CFU/g, echinodermata 5.47±0.50 CFU/g, fish 5.021±0.38 CFU/g, and mollusc 5.03±0.65 CFU/g. The logarithmic mean of enterococci was 2.36±0.37 CFU/g, and in accordance with fish and shellfish, crustacean 3.44±0.12 CFU/g, shellfish 3.87±0.45 CFU/g, echinodermata 3.38±0.0 CFU/g, fish 2.16±0.41 CFU/g and mollusc 0.01±0.0 CFUIg. The logarithmic mean of vibrios was 1.60±0.59 CFUIg, and in accordance with fish and shellfish, crustacean 4.23±0.11 CFU/g, shellfish 3.58±0.90 CFUIg, echinodermata 1.64±0.34 CFU/g, fish 1.79±0.67 CFU/g and mollusc 1.07±0.61 CFU/g. The logarithmic mean of staphylococci was 1.60±0.59 CFU/g, and in accordance with fish and shellfish, shellfish 0.01±0.00 CFU/g, echinodermata 3.51±0.60 CFU/g, fish 1.68±0.64 CFU/g, crustacean 0.34±0.33 and mollusc 2.90±0.11 CFU/g. The logarithmic mean of coliforma was 2.2±0.32 CFU/g, and in accordance with fish and shellfish, echinodermata 3.58±0.89 CFU/g was highest, shellfish 3.25±0.30 CFU/g, crustacean 3.23±0.49 CFU/g, fish 2.18±0.63 CFU/g, peeled shellfish 1.80±0.51 CFU/ g and mollusc 1.55±0.95 CFU/g. As the results of research of the change of the contaminated indicator microflora in working with storage period at 10℃, 20℃ and 30℃, total viable counts was increased without storage temperature and enterococci were decreased slowly at 10℃, but increased at 20 and 30℃ Vibrios were decreased slowly at 10℃, decreased at 20℃ and 30℃ in 2 days after increased rapidly. Staphylococci were increased promptly without storage temperature in 2 days, then the total viable counts were maintained. Coliforms were increased at 10℃ by 7 days, then decreased or maintained after 14 days, changed at 20℃ in accordance with fish species in 2 days, then returned to the initial total viable count, and decreased rapidly at 30℃ on 2 days. By the way, there were no difference among the species.