This study was carried out to evaluate the microbiological characteristics of retail chicken meats stored under various conditions. Nine of whole chickens and nine of chicken breasts were used for bacterial analysis. Each chicken meat was divided into subsamples of 25 g each followed by storage at room temperature (25℃), refrigeration temperature (4 ℃), and freezing temperature (—20℃) for 180 min, 5 days, and 3 days, respectively. The standard plate counts were performed for the enumeration of the total aerobic bacteria. The number of aerobic bacteria was gradually increased by 1 log in samples held at the room temperature for 180 min. There was statistical difference in the number of bacteria between at 0 min and at 180 min of storage. For samples stored at 4℃ for 5 days, the number of bacteria was increased from 5.11 to 7.26 log CFU/g in chicken breast and 3.83 to 6.04 log CFU/g in whole chicken with statistical difference. No significant changes were observed in frozen chicken. The results of this study may provide useful information to consumers for proper storage and safe handling of chicken meats.

느타리버섯의 생육단계에서 재배온도 및 상대습도를 조절하여 선도 유지 기간을 연장하기 위해 수행한 결과, 생육온도별 저장기간에 따른 버섯의 수분함량은 상대습도가 낮은 처리에서 다소 낮았다. 생육조건별 중량감모율은 저장기간이 길수록 증가하였으며, 처리간 큰 차이 없었고, 갓신장율은 저온생육시에는 상대습도 95±5% 처리에서 저장 21일에 가장 높았으며, 대조구에서는 상대습도 80±5% 처리에서는 저장 24일에 가장 높았다. 버섯의 물성을 분석한 결과, 경도와 탄성은 저장기간이 길수록 낮아지는 경향이었으며, 상대습도 및 생육온도에 의한 차이는 거의 없었다. 따라서, 느타리버섯을 발이유기 후 생육온도 13℃, 상대습도 80%이상으로 조절하여 생육하여 250~300g 랩포장하여 4±1℃에 저장 했을때 24일까지 신선도를 유지할 수 있었다.

This study was carried to investigate the suitable conditions of Pleurotus eryngii through precooling for the more long-term freshness. The time to be same temperatures between P. eryngii and storage room through precooling at 0℃ and 4℃ were showed 2 hours and 5.5 hours, respectively. P. eryngii was cooled within 6 hours and 18 hours at 0℃ and 4℃ with two type, forced air cooling and pressure cooling. After precooling, P. eryngii was packed 400±10g with anti-fog film, and then stored at 4±1℃. In all treatment of precooling, the weight loss was peaked at 25 days and hardness of P. eryngii were decreased at 10 days during storage. There were no significant freshness differences between precooling type and conditions. It was found that the optimum stored period of P. eryngii at 4℃ after precooling was estimated to be 30~35days. Consequently, it is necessary to elucidate efficient and economic precooling conditions.

In order to establish an indoor-rearing system for A. Alcinous, the effect of temperature and pupal diapause was investigated. Temperature has been suggested as an important factor in determining the developmental rate, Lifespan and mortality in invertebrates. As temperature increase, the developmental period was gradually reduced. The developmental period of A. alcinous larvae was 12.9 days and 25.8 days at 30℃ and 20℃, respectively. And we investigated the effect of different chilling days on diapause termination. Under longday condition(LD 16:8h, 20℃) oxygen consumption gradually increase. But oxygen consumption on the first day of pupa was the highest and rapidly fell down the next day and attained lowest point after 7days, with average value of 1.92mol/min/g under shortday condition(LD 8:16h). Diapause pupae were chilled for 60, 75, 90, 105 days at 8℃ and thereafter subjected to LD 16:8h at 25℃. And emergence rate at 60~90days was 57.1~80.7%.

This study was conducted to investigate effect of low temperature and ripeness stages on fruit quality of chilli pepper (cv. Nockgwang) during storage at 5, 7, and 10 . Fruits ℃ at three ripeness stages were selected based on uniform fruit size and three maturities on peel color: S1 (fully developed fruit just before the onset of ripening), S2 (fruits in the onset of color change), and S3 (fruits in completely red in color). Lower temperature attributed to increase fruit weight loss (WL) and WL was higher in S2 than the other ripeness stages. The highest respiration rate and ethylene production was found at S2 fruit while those of fruit in green and red showed similar and remained lower level during storage. Electrolyte leakage (EL) was higher as storage temperature decreased. After 28 d storage, EL rates of fruits at 5℃ were 43, 36, and 17% in S1, S2, and S3 while those at 10℃ were 17, 19, and 14%, respectively. These results show that chilli pepper fruits are tended to lose more water at lower temperature partially associated with increased EL. Thus, threshold storage temperature must be considered for avoiding chilling during storage and for extending the storage life of fresh pepper fruits.

신선 퉁퉁마디의 저장성 향상을 위해 MAP에 적절한 포장재와 저장온도를 구명하고자 본 실험을 수행하였다. 저장중 생체중 감소는 10℃ 이하의 저온과 무공필름에서는 2% 이하로 유지되었으나 25℃의 유공필름처리는 4% 이상의 감소를 보이면서 품질저하가 발생하였다. 저장중 포장재내 대기조성은 필름 투과율에 비례하였는데 이산화탄소는 1~2%, 산소는 15% 이상으로 유지되었다. 25℃를 제외한 모든 처리에서 저장 중 포장재내 에틸렌 농도는 7일 이후 급격히 증가하였는데, 2℃에서 가장 높아 저온 장해가 발생한 것으로 추측되었다. 저장 최종일에 이취와 부패율은 5℃에서 가장 낮았으며, 포장재별로는 다른 양상을 보였는데 저장온도중 가장 낮았던 5℃에서는 50μm 두께의 ceramic 필름이 가장 낮은 이취와 부패율을 보였다. 외관상 품질로 본 저장수명 역시 5℃에서 50μm ceramic 필름으로 포장하였을 때 28일 이상으로 가장 길었다.

A commercial egg parasitoid, Trichogramma sp. Nabis101, was released into agricultural cultivating areas in Korea due to its wide host spectrum against insect pests. Moreover, an application technique has been recently developed to enhance its control efficacy by mixture treatment with a microbial control agent. Despite its expansion of commercial availability, any genetic identification on this commercial strain was not determined. Also, to meet inconsistent demands from consumers, the live parasitoids need to be stored without significant loss of their survival and parasitic activity. This study determined nucleotide sequence of internal transcribed spacer (ITS) of the wasp species. The identified ITS sequences indicate that this wasp species is most similar to T. brasiliensis. Optimal storage condition of this wasp required young parasitized stage at 10℃. Under these conditions, survival, sex ratio, longevity, and parasitic behavior were not much impaired for 5 weeks.

The principal objective of this study was to develop and standardize a preparation method for low-sodium tomato jangachi (traditional Korean pickle) via short-term fermentation with immature green cherry tomatoes. In order to determine the preferred concentrations of soy sauce and soaking temperatures of immature cherry tomato pickles in different stages of storage, we conducted an analysis of physico-chemical characteristics and microbiological properties, and also performed a preference test on samples of immature green cherry tomato pickles. Immature cherry tomatoes were prepared in three different soy sauce concentrations --20, 40, and 60%-- and three different soaking temperatures --60, 80, and 100℃-- and then stored for 28 days at 5℃. As a result, the pH increased significantly with increases in the amount of soy in the dipping solution (p〈0.05). The saltiness was maintained at levels of approximately 0.17~0.28% (20% group), 0.32~0.67%% (40%group), 0.48~1.00% (60% group) during storage periods. These results show that the saltiness of immature cherry tomato pickles was substantially lower than that of commercial pickles. The contents of reducing sugar and lightness decreased significantly with increasing concentrations of soy dipping solution. The redness and yellowness values of the tomatoes decreased significantly with increasing concentrations of soy sauce. Additionally, the lower the concentration of soy sauce used, the more rapidly the hardness of the immature cherry tomato pickles was reduced at 100℃. PME activity moved within a narrow range, and then stabilized during the storage period. With regard to the results of the consumer preference test, 20%-100℃ was the most preferred condition overall, 40%-80℃ was the condition in which the texture was most preferred, and 40%-80℃ was the condition that yielded the highest color scores.

Rearing media were selected for the production of sterile larvae of greenbottle blowfly, Phaenicia (=Lucilia) sericata (Meigen) which is widely used in maggot therapy. Eight media available in the market were used in this study. Egg hatchability was not different among the media. Survivorship of the larvae was higher in blood agar (BA), sabouraud dextrose agar, and brucella blood agar than the others. A higher content (20~40%) of sheep blood in BA and chocolate agar increased the survivorship of larvae. The eggs and the early 3rd larvae could be stored for 12 days at 8℃ and for 15 days at 6℃ without decrease in hatchability and larval survivorship, respectively.

본 연구는 주요 냉장식품(두부, 묵, 어육가공품(어묵), 생면, 김초밥, 샐러드)의 온도 관리실태를 조사하여 문제점을 파악하고 국내 유통업소의 냉장·냉동진열 판매 제품의 관리온도 개선 및 관리방안을 제시하고자 하였다. 주요 냉장고 내 위치별 온도는 보편적으로 아래(7.5 ℃)가 높고 윗부분(6.4 ℃)이 낮았으며, 앞쪽(8.9 ℃)이 높고 뒤쪽(7.5 ℃)이 낮은 것으로 조사되었다. 모든 냉장식품의 평균 냉장고 보관 온도는 7 ℃(최대 22.9 ℃, 최저 -2 ℃)였으며, 대형할인매장이 6.5 ℃, 백화점이 7.5 ℃, 편의점이 7.6 ℃ 였다. 모든 냉장식품의 평균 표면온도는 8.2 ℃(최대 19 ℃, 최저 -0.4 ℃)였으며, 대형할인매장이 8.1 ℃, 백화점이 8.5 ℃, 편의점이 7.6 ℃으로 조사되었다. 식품표면과 중심온도는 식품별 차이가 있으며 최대 2.3 ℃ 차이로 나타났다. 두부(표면온도 6.1~10.6 ℃, 중심온도 4~11.1 ℃), 어묵(6~12.3 ℃, 6.5~13.6 ℃), 김밥(2.3~18.2 ℃, 2~17.3 ℃), 샐러드류(4.6~12.2 ℃, 4.1~10.4 ℃), 생면류(3.4~12.6 ℃, 3.8~10.3 ℃), 묵류(7.1~19 ℃, 8.3~17.1 ℃)의 다섯 가지 식품별로 차이를 보였다. 냉장고 표시온도와 식품표면 온도 항목간의 유의적 차이가 있는 것으로 나타났다(P<0.01). 냉장고 표시 온도는 김밥 1곳(100%)에서 규정 온도인 10 ℃를 초과했지만, 식품표면 온도에서는 두부 9곳(17%), 어묵 6곳(11.3%), 묵 10곳(18.9%), 생면 11곳(20.8%), 김밥 13곳(24.5%), 샐러드 4곳(7.5%)이 초과한 것으로 나타났다. 결론적으로 우리나라 냉장보관 식품 중 표면온도와 10 ℃를 초과하는 경우가 많아 냉장보관식품의 온도관리가 철저히 이루어져야 할 것으로 판단된다.

콩나물로부터 고 isoflavone 함량의 콩 식품원료를 얻고자 콩나물 재배 시 빛을 쪼여주고, 재배한 콩나물의 저장온도를 달리한 조건에서 isoflavone 함량변이를 조사하였다. 공시 품종들의 7일 재배 후 저장하기 전 isoflavone 함량은 아가 3호>아가 1호>아가 2호>아가 4호>장기콩>보석콩>은하콩>풍산나물콩 순이었는데 아가 3호의 isoflavone 함량은 이었다. 재배한 콩나물의 isoflavone 함량은 저장온도에 따라 품종별로 상이한 차이를 보였는데, 저장온도 와 에서 isoflavone 함량이 높은 반면 저장온도 와 에서는 대부분 콩 품종들의 isoflavone 함량은 낮았다. 저장일수에 따른 isoflavone의 함량은 품종들 간에 일정한 경향이 없었으나 대부분의 품종들은 저장기간 3일 이내에 가장 높은 isoflavone 함량을 보였고, 아가 4호와 장기콩은 저장기간 5일에서도 높은 isoflavone의 함량을 보였다. 저장기간과 온도처리를 하여 재배한 콩나물의 isoflavone 함량을 높이고자 한 결과 아가 3호를 포함하여 공시한 8품종 중 4품종에서 에서 하루 동안 저장했을 때 가장 높은 isoflavone의 함량을 나타내었다. 재배한 콩나물들 중 저장을 통해 가장 높은 isoflavone의 함량을 보인 품종은 아가 3호로써 저장후의 isoflavone 함량은 이었다. 본 연구에서는 콩나물 저장기간 동안 수분공급이 지속적으로 이루어졌기 때문에 콩나물이 계속 성장한 것으로 생각된다. 따라서 저장기간 동안 온도처리를 달리하여 isoflavone의 함량을 증가시키는 보다 확실한 방법을 확립하기 위해서는 더 많은 연구가 이루어져야 할 것으로 생각되어진다.

The purpose of this study was to investigate the antibacterial effect of films on shelf-life and microbiological Safety of pork meat. Effects of antimicrobial films against total aerobic bacteria, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium, Staphylococcus aureus in pork meat were evaluated during storage of 14 days at 5℃, 10℃ and 15℃. Antimicrobial films were developed with addition of a natural substance, wasabi extracts (Wasabia japonica). At 5℃ storage, growth of total aerobic bacteria, E. coli O157:H7, L. monocytogenes were inhibited higher than at 10 and 15℃. Especially, the numbers of S. Typhimurium and S. aureus were increased gradually at 5℃ even in the control sample, and it takes more than 14 days to increase in every sample upto 6 log10 cfu/g. The higher antimicrobial effects of the films were observed at storage of 5℃ than at 10oC and 15℃. There was a limit of a single treatment of antimicrobial film to prolong shelf-life of pork meat. The synergistic effect of antimicrobial film were observed with addition of refrigeration at 5℃.

꼬투리째 먹는 채소인 풋꼬투리 강낭콩의 수확후 포장 및 저장 유통온도별 품질보존 및 상품성에 미치는 영향을 조사하였다. 저장중 중량의 변화에서 PP필름 포장시는 변화가 없었으나 골판지박스 포장시는 저장 온도가 높고, 저장기간이 길어질수록 중량 감소율이 현저하였다. 저장중 경도는 저온저장이 상온저장보다 높게 유지되었다. 비타민 C함량은 저장중 모든 처리에서 감소되었고, 그 감소의 폭은 상온저장보다는 저온저장 시 적었다. 엽록소함량은 비닐필름 포장시는 저장온도간에 차이가 없었으나, 박스포장시는 상온저장시 엽록소함량의 감소가 저온저장보다 컸다. 외관 상품성을 표시하는 신선도는 비닐포장이나 골판지박스에 속비닐 넣은 후 산물을 포장하여 8~10℃정도에서 저장 유통시는 상품유지기간이 8일 정도로 상온보관대비 4일정도 연장되었다.

In this study we attempted to analyze the change of protein and mycelial activity during low temperature storage progressed. At first, esterase and peroxidase isozymes were detected at third and fourth subculture in Pleurotus ostreatus and peroxidase isozymes in Pleurotus eryngii were detected at first and second subculture but esterase isozymes showed almost same band patterns subculture advanced. The decolorization ratio of media color including indicator were decreased according to subculture advanced in P. ostreatus and P. eryngii. However there were not significantly difference of mycelial growth, fruit body yield, esterase isozyme band pattern and laccase activity of P. eryngii according to storage periods at low temperature. Therefore, it was possible to store sawdust spawn of P. eryngii at 4℃ for 120days.

신선 엽채류인 시금치의 수확 후 포장, 저장유통온도별 품질보존 및 상품성에 미치는 영향을 조사하였다. 저장 중 중량의 변화에서 포장 후 저장 시는 중량감소가 거의 없었으나, 무포장은 저장기간이 길어질수록 그리고 저장온도가 높을수록 감소율이 현저하였다. 비타민 C함량은 저장 초기에 감소가 컸으며, 저장온도가 낮을수록 감소가 적었고, 비닐포장 간에는 큰 차이가 없었다. 시금치에서 Hunter's value는 저장온도가 높을수록 b값이 높아지는 경향이었다. 시금치 저장 중 외관 신선도에서 polypropylene 비닐포장 후 저온(1~3℃) 저장은 최고 34일, 상온(10~15℃) 저장은 8일까지 상품성이 유지되었으나, 무포장 상온보관은 3일 밖에 유지되지 않았다.