본 연구는 고부가가치 약용작물인 잔대(Adenophora triphylla var. japonica Hara)의 종자 형태특성과 휴면 유형을 조사하고, 발아율 향상을 위한 소독제와 광질을 선발하기 위해서 수행되었다. 종자 소독은 증류수(대조구), NaClO 4%, H2O2 4%와 benomyl 500mg·L-1를 이용하여 실시하였으며, 광질처리는 암조건(control I), 형광등 (control II), LEDs[red, blue, green, RB LEDs(red:blue = 8:2, 6:4, 4:6, 2:8)]를 광주기 12/12(light/dark), 광도 150μmol·m-2·s-1 photosynthetic photon flux density로 설정 하였다. 잔대 종자는 emryo (E):seed (S) ratio가 0.4로 미숙배 종자지만 30일 이내에 발아가 되며, 침지 6시간 만에 포화상태에 도달하였다. 종자 소독 후 benomyl 처리에서 곰팡이 발생이 유의적으로 억제되었으며, 최종 발아 율이 87%로 가장 높았다. 적색광에서 최종 발아율이 92%로 가장 높았으며, 일일평균발아수는 R2B8에서 가장 적었다. 따라서 잔대 종자는 휴면이 거의 없으며, benomyl 소독제와 적색광이 발아율 향상에 효과적이여, 향후 약용작물 잔대 재배에 이용가치가 높을 것이라 판 단된다.

This study was carried out to investigate the antioxidant activities and the contents of antioxidant compounds in extracts derived from 23 medicinal crops. Dried plant materials were extracted by 80% methanol and the extracts were used to experiment. The levels of total phenolic compounds were much higher in Oenothera biennis, Houttuynia cordata, Cedrela sinensis, Rhus javanica, Artemisia annua, Reynoutria sachalinensis, and Dendropanax morbifera compared with other crops. On the other hand, the total phenolic compound levels in Carthamus tinctorius, Polygonum multiflorus, Ginko biloba, Solanum nigrum, and Saposhnikovia divaricata were relatively low. The total flavonoid contents were highest in H. cordata, followed by D. morbifera, A. annua, A. continentalis and O. biennis. DPPH activity was significantly higher in A. annua, R. javanica, C. sinensis, D. morbifera and O. biennis, and ABTS activity was significantly higher in R. javanica, C. sinensis, O. biennis, R. sachalinensis, A. annua, and D. morbifera than other plants. These results suggest that O. biennis, A. annua, R. javanica, D. morbifera, and R. sachalinensis can be utilized as food additives and natural antioxidants due to their strong antioxidant activity.

본 연구는 중금속으로부터 안전한 약용작물을 생산하기 위한 시비방안을 제시하기 위해 토양의 화학 적 특성과 식물이 이용 가능한 형태의 중금속 함량의 관계를 구명하고자 실시되었다. 전국의 173개 지 점에서 재배중인 약용작물 재배지의 토양을 채취하였으며 1M NH4OAc로 식물이 이용할 수 있는 형태의 중금속인 비소(As), 카드뮴(Cd), 납(Pb)의 함량과 토양의 화학적 특성을 분석하였다. pH가 증가함에 따 라 식물이 이용할 수 있는 형태의 Cd과 Pb은 감소하였지만, As는 증가하였다. 유기물 함량이 증가함에 따라 식물이 이용할 수 있는 Cd와 Pb의 함량은 증가했지만, As는 감소하였다. 유효인산의 함량이 증가 함에 따라 식물이 이용할 수 있는 형태의 As 함량은 증가했지만 Cd과 Pb의 함량은 감소하였다. 위의 결과들은 토양의 pH, 유기물 함량, 인산함량의 차이에 따라 식물이 이용할 수 있는 As의 함량과 식물 이 이용할 수 있는 Cd, Pb의 함량이 대조적으로 나타나는 경향을 명확하게 보여준다. 따라서 약용작물 재배지에서 중금속 안전 농산물을 생산하기 위해서는 대상으로 하는 중금속의 종류를 설정 후 시비를 통한 토양의 pH, 유기물 함량, 유효인산 함량의 관리가 필요할 것으로 판단된다.

국민의 건강과 웰빙에 대한 관심 증대 및 소득 증가로 한약재 소비를 비롯한 식품과 화장품 원료로서의 산업적 가치가 커지면서 약용작물의 수요는 점차 증가할 것으로 예상하며 한, 중 FTA 체결로 인해 약용작물 시장개방의 수입증가는 위협요인으로 작용할 것이다. 그러한 측면에 서 약용작물을 포함한 농식품산업을 미래 성장 동력산업 으로 육성하기 위해서는 종자품종육성과 생산유통기반의 정비와 확충, 산지이용규제 완화 등의 다양한 대책이 마련되어야 한다. 본 연구 결과의 활용방안을 살펴보면 다음과 같다. 첫째, 국가의 약용산업 육성 중장기 전략과 종자관리 체계의 구축에 필요한 약용작물의 데이터베이스를 구축 하고 향후 개발해야 할 중점기술을 제시해야 한다. 둘째, 우수한 약용작물 품종 개발 및 보급으로 농업생산성을 높여야 한다. 셋째, 약용작물 종자생산 및 보급에 필요한 제도정비가 필요하다. 넷째, 우량종자의 표준화 및 규격화 로 약용작물의 생산유통체계를 확립해야 한다. 현재 약용 작물의 소비행태는 직접적인 섭취보다 화장품, 의약품, 식품의 형태로 소비되는 패턴으로 바뀌고 있으며 규모가 점점 확대될 것으로 전망된다. 그러므로 향후 약용작물의 생산에서는 이러한 산업화 추세와 변화에 대응한 정책개 발과 제도적인 개선이 뒷받침되어야 한다.

최근 약용작물 재배 종류와 면적 증가로 병해충 발생 종도 늘어나고 관리비용이 증가하고 있지만 약용작물에서 병해충 안전관리를 위해 등록된 약제는 매우 부족 한 실정이다. 2010년부터 2014년 상반기까지 제천, 음성, 평창 등 약용작물 재배지 내 감초, 당귀, 황기, 도라지, 방풍, 삽주 등에 발생하는 해충 종류, 피해 증상 및 특징 들을 조사하였다. 감초에서는 알락수염노린재, 아카시아진딧물 담배거세미나방, 차응애 등, 황기에서는 아카시아진딧물, 싸리수염진딧물, 왕담배나방, 노린재류 등, 방풍에서는 당귀장님노린재, 어수리애기잎말이나방, 차응애 등, 당귀에서는 꼬부랑진딧물, 어수리애기잎말이나방, 당귀장님노린재 등, 도라지에서는 도라지 수염진딧물, 점박이응애 등, 삽주에서는 우엄수염진딧물, 조팝나무진딧물 등 다양 한 종류의 해충들이 발생하고 있었다. 특히 대부분 약용작물 재배 초기인 5∼6월에 는 신초를 중심으로 진딧물, 응애, 총채벌레 류 등의 발생이 확인되었다. 작물 재배 후기인 8∼10월에는 왕담배나방, 파밤나방, 알락수염노린재, 톱다리개미허리노 린재 및 딱정벌레 류 등이 주요 발생 해충으로 주로 신초와 열매 부위에 발생하여 피해를 유발하고 있었다.

최근 약용작물 재배 증가로 병해충 관리비용이 급증하여 병해충 신속･정확한 진단기술 개발이 시급한 실정이다. 또한 국내 약용작물 가공식품 및 건강식품 수요가 증가에 따른 안전 농산물에 대한 요구로 화학농약 사용 절감 및 잔류를 절감할 수 있는 친환경 방제기술 개발이 요구되고 있다. 이러한 약용작물 재배 종류 및 생산량 증가로 인하여 재배 현장에서 발생하여 문제가 되고 있는 병해충의 종류 또한 매우 다양하여지고 있어, 약용작물별 해충 발생 생태 및 작물별 피해 구명 및 신속･정확한 진단기술 개발을 위한 기초 연구가 절실하다. 2011~2012년 제천, 음성, 평창, 청양 등에서 당귀, 황기, 도라지, 더덕, 맥문동, 방풍, 삽주, 오미자 등 약용작물에 발생하는 총채벌레 발생 종 조사 및 피해 특징들을 조사하였다. 총채벌레의 경우 약용작물에 발생할 경우 작물 생육에 피해를 유발하는 문제 해충으로 대부분의 조사 대상 약용작물에서 발생이 확인되었다. 특히 재배 초기에 어린 신초(5~30%/전체 신초)나 엽 등에 발생하였을 경우 작물의 생장을 위축시켜 많은 피해를 유발하고 있었다. 약용 작물별 발생하는 총채벌레의 정밀한 종 동정을 위하여 기주식물별 채집한 총채벌레들을 PCR 기법 이용 분류･동정 진행한 결과 볼록총채벌레, 파총채벌레, 대만총채벌레 등 기주식물별로 다양한 총채벌레들의 발생을 확인하였다.

최근 약용작물 재배 증가로 병해충 관리비용이 급증하여 병해충 신속․정확한 진단기술 개발이 시급한 실정이다. 또한 국내 약용작물 가공식품 수요가 증가 및 안전 농산물에 대한 요구로 화학농약 사용 절감 및 잔류를 절감할 수 있는 친환경 방제기술 개발이 필요하다. 2010년부터 제천, 음성, 평창 등에서 당귀, 황기, 마, 맥문동, 도라지 등 약용작물에 발생하는 주요 해충들의 발생 및 패해 특징들을 조사하였다. 약용 작물 재배 기간 중 지상부에 발생하여 피해를 유발할 수 있는 해충 종으로는 총채벌레, 응애, 진딧물, 나방, 노린재, 딱정벌레, 잎굴파리 등으로 각 작물의 재배 시기 및 형태별로 다양하게 발생 피해를 유발하고 있었다. 특히 재배 초기(5~7월) 진딧물, 응애, 총채벌레 등은 적절한 관리가 행하여지지 않을 경우 부분적으로 많은 피해(10~30%/주)를 유발하고 있었다. 작물 재배 후기(8~10월)에 발생하여 피해를 주로 유발하는 해충들로는 나방 유충, 노린재, 딱정벌레 류 등으로 주로 지상부 순 끝부분과 열매부위를 가해하여 피해(5~10%/주)를 유발하고 있었다. 재배기간 중 약용작물 근권부를 가해하는 해충으로는 선충 및 파리유충 등 소량 발생하여(2~3%/주) 수확물의 감소를 유발하고 있었다.

Background : Great achievements have been made to form a system to protect seed intellectual property rights over the past years in Korea. The system is divided into two parts: the plant variety protection (PVP) system and the patent. Korea has a dual protection system unlike Europe. The purpose of this study was to examine the application and industry trends, and the registration states of herbal crops under the PVP system. Methods and Results : Eighteen years have passed since Korea introduced PVP system. The number of plant varieties were applied for the registration were 9,959 and 7,070 among 9,959 were registered until the end of 2017. The total number of 9,959 applicant varieties formulations like ornamental crops (5,036 varieties, 52%) > vegetables (2,149, 22%) > food crops (1,251, 13%) > persimmon trees (626, 6%) > special crops (397, 4%) > mushrooms (267, 2%) > and forage crops (69, 1%). The total number of 7,070 registered varieties formulations like ornamental (3,779 varieties, 54%) > vegetables (1,344, 19%) > food crops (1,020, 15%) > persimmon trees (398, 5%) > special crops (282, 4%) > mushrooms (169, 2%) > and forage crops (42, 1%). Among the applicant varieties for registration, special 50 type crops in 397 varieties were applied until the end of 2017. The applicant varieties formulation like sesame (72 varieties) > perilla (54) > peanuts (53) > ginseng (32) > Tea tree (18) > rape (31) > matrimony vine (14) > mulberry (11). Among the remained 42 crops, less than 10 varieties were applied. Oilseed crops such as sesame, perilla, peanut and rapeseed are 60% of the total number of applications for special crops, 32% of herbal crops such as ginseng and foxglove (Rehmannia glutinosa), 8% in fiber (ramie) and beverage crops (stevia, tea). Compared with Japan, 33,206 varieties were applied and 8,963 were registered as of March 31, 2018. Among the special crops, 287 varieties (0.8%) were applied and 126 varieties (1.4%) were registered. Conclusion : The total of 9,959 varieties have been applied for the PVP, of which 397 (4%) are special crops and 282 (4%) among 397 are registered varieties. The number of applications of herbal crops such as ginseng and foxglove consist 165 varieties, which corresponds to 2% of total applications. Compared with Japan in the term of registered special crops, in Korea they have been registered about twice as much.

Background : This study was conducted to investigate the production competitiveness of medicinal crop. The results of this study were intended to be used as basic data for establishing the direction of R&D needed for domestic medicinal crop farming system. Methods and Results : For data analysis, frequency, percentage and average and Chi square (× 2) value were used. The survey showed that the cultivated crops of the respondents were medicinal crop. Firstly, there was a significant difference in farming disability by farming career. The 'cultivation method (60%, 42.1%)' was high in farming preparer and beginner (≤ 3 years), but the disability in 'cultivation method' decreased as the farming career was longer. Respondents who had more than 10 years of farming career complained of 'climate problem (24.0%)' followed by 'cultivation method (20.0%)' and 'pest control (20.0%)'. There was also a significant difference in the farming disability by medicinal crops farming career. The 'cultivation method (50.0%)' was the highest of the farmers who had 1 year of medicinal crops farming career, however the respondents with more than 4-ears of career complained of 'climate problems (27.3%)' and 'pest control (23.6%)', but 'cultivation method' was low as 18.2%. Secondly, there was a difference in sales disability by farming career, and cross-sectional analysis was statistically significant at × 2 = 41.320. The respondents who were preparer for farming had the biggest sales disability at 'shortage of market (44.4%)', and the rates decreased gradually as the farming career increased. Respondents more than 10-ears had the biggest sales disability as ‘uncertain market price (50.0%)’, and 'shortage of market' was low as 12.5%. Cross-sectional analysis of sales disability by medicinal crops farming career showed that × 2 was 49.705, which was statistically significant. Farmers with no career in cultivating medicinal crops had the biggest sales disability at 'shortage of market (40.0%)' and farmers with more than 4 years of career complained of 'uncertain market price (42.2%)'. Lastly, there was a statistically significant difference in cultivation performance by medicinal crops farming career. The respondents with a career of less than 1 year had the highest proportion at 'medium (48.5%)', but those with more than 4-ears of career had the highest rate of 'creation of profit (43.1%)'. Conclusion : As farming career and medicinal crops farming career increased, environmental factors such as climate and pest problems affected in cultivation stage significantly, further uncertain market price gave a large factor in sales stage.

Background : It is necessary to develop a diagnostic tool for the precision agriculture according to the change of agriculture environment. In particular, medicinal crops are often cultivated in poor areas such as mountains and valleys, and are susceptible to abnormal climates. Therefore, there is a need for a method that can diagnose the growth easily and with time. We conducted a study to investigate the possibility of remote sensing for 10 species of medicinal crops using unmanned aerial vehicles and multispectral images. Methods and Results : This study was conducted in 0 - 4 times continuously cultivated field with 10 species of medicinal crops (Angelica gigas, A. koreana, A. tenuissiman, A. dahurica, A. acutiloba, A. polymorpha, Ledebouriella seseloides, Lithospermum erythrorhizon, Rhemannia glutinosa). Growth characteristics such as plant height, number of leaves, leaf length, leaf width, stem diamanter and number of stems were investigated between July 10 and July 16. The reflectance map of red (660 ㎚) and NIR (790 ㎚) was obtained using a UAV equipped with a multi-spectral sensor and NDVI [Normalized Difference Vegetation Index] was obtained with a Pix4D mapper. As the number of times of continuos cultivation increased, the growth and NDVI tend to decreased. 6 species of Angelica genus were positively correlated with growth characteristics such as plant height, leaf length, leaf width, and stem diameter and NDVI. Otherwise, there was no significant relationship between growth characteristics and NDVI in Ledebouriella seseloides, Lithospermum erythrorhizon, and Rhemannia glutinosa. This is because the difference in the growth according to time of continuos cultivation was not significant and the canopy structure tended to overlap with the upper and lower leaves. Conclusion : NDVI is widely used as a useful tool for evaluating the canopy productivity and is highly correlated with the above-ground growth characteristics of 6 species of Angelica spp. It is necessary to develop the growth index suitable for the each species and to enhance the validity of growth index by examining the correlation under various cultivation conditions.

이 연구는 도라지의 유기재배를 위하여 3년근 이식 예정지에 풋거름 작물 재배에 따른 도라지 뿌리의 생육특성 및 사포닌 함량에 미치는 영향을 구명하고자 실시되었다. 생육특성에서는 기재배지에서의 뿌리의 직경 및 곁뿌리 수는 호밀 재배구에서 가장 굵거나 많았으며, 뿌리 생체중은 풋거름작물 재배구간 차이는 없었으나, 대조구에 비해 높게 나타났다. 도라지 뿌리의 무기성분 함량에서는 무기성분별 유의성이 있는 것으로 나타났으며 특히, 기재배지에서의 호밀 처리구에서 높은 함량이 나타났다. 사포닌 함량의 변화에서는 기재배지에서는 호밀 재배구에서 모든 사포닌 함량이 가장 높은 함량으로 나타났으며, 새재배지에서는 헤어리베치 재배구에서 높은 함량을 나타냈다. 대조구에 비해 풋거름작물 재배구의 기재배지와 새재배지 모든 재배구에서 사포닌의 함량이 높게 나타났다. 따라서 도라지 유기재배 시 풋거름작물에 따른 뿌리의 생육 특성 및 약리성의 변화 등의 유의적인 차이가 나타나는 것을 확인할 수 있었으며, 연차 간 재배 시 호밀 재배구에 생육 및 약리성이 우수한 것으로 나타났다.

Background : Recently there has been an increasing demand for well-being and health food products. Foreign special and medicinal crops which were not cultivated in Korea before are widely introduced and cultivated in Korea. Therefore, in order to provide the basic information necessary for stable cultivation of the introduced crops, we conducted a survey on the actual condition of cultivation of farms and sought to suggest future work. Methods and Results : The survey was conducted in cooperation with the Rural Development Administration and local rural organizations in the year 2015 based on cultivation area, number of farms, and cultivation scale. In thes case of Lepdium meyenii and Asimina triloba, we surveyed the status of management technology by stage of cultivation through the field case study of farms. As a result, 14 crops were cultivated in 1,015 farms in 329.9 ha area including, Allium hookeri, Amaranthus blitum, Annona muricata, Asimina triloba, Chenopodium quinoa, Curcuma longa, Glycyrrhiza uralensis, Gynura procumbens, Lepdium meyenii, Moringa oleifera, Ocimum americanum, Psidium guajava, Silybum marianum, and Stachys japonica. The cultivation scale decreased in the order of C. longa, G. uralensis, A. hookeri, P. guajava, L. meyenii, M. oleifera, and A. triloba. Generally, L. meyenii seeds were sown generally in September and the roots were harvested from mid April to early May in the following year after wintering. A. triloba was cultivated using emerged seedlings or grafted seedlings. The emerged seedlings were more 5 years, and the grafted seedlings were more 4 years so that the fruit can be harvested. Conclusion : In order to stabilize the cultivation of special and medicinal crops introduced from foreign countries, it should be safe and economical in terms of cultivation environment based on domestic cultivation adaptability test, and the use of products and sales channels should be established first. Also, national support and countermeasures for benefit sharing under the Nagoya protocol are needed.

Background : The development of an antioxidant to prevent disease by ROS-induced oxidative stress is necessary. This study investigated the changes of antioxidant capacities of two medicinal crops extracts by lactic acid fermentation. Methods and Results : The changes of free-radical scavenging activity of medicinal crops extracts by lactic acid fermentation were evaluated by using DPPH free-radical scavenging assay and ABTS free-radical scavenging assay. The DPPH free-radical scavenging activity of extracts or lactic acid fermented extracts were estimated as followed. samples were thoroughly mixed with 1 ㎖ ethanol solution of 0.1 mM DPPH. After stand for 30 min in the dark, the absorbance was measured at 570 ㎚ by using a UV Spectrophotometer. ABTS scavenging activity of extracts or lactic acid fermented extracts were estimated as followed. The working solution was prepared by mixing 1 ㎖ of ABTS solution with 88 ㎖ of 50% ethanol. A total of 25 ㎕ of samples were mixed with 225 ㎕ of ABTS working solution and allowed to stand for 10 min. The absorbance was read at 732 ㎚ in a UV spectrophotometer. The data were showed that lactic acid fermented extracts were higher antioxidant ability than the extracts. Conclusion : This study was showed that the antioxidant capacities of two medicinal crops extracts were improved by lactic acid fermentation.

Background : This experiment was conducted to improve the farm income by decreasing the rate of corrosion and increasing the emergence rate by selecting the proper storage method of the medicinal crop seed root. Methods and Result : Ten kinds of medicinal crop seed roots of Glycyrrhiza uralensis Fischer, Salvia miltiorrhiza Bunge, Codonopsis lanceolata, Platycodon grandiflorum, Atractylodes japonica, Angelica acutiloba, Adenophora triphylla, Rehmannia glutinosa, Angelica gigas and Cnidium officinale were used as test materials. In experiment 1, storage experiments were carried out using four treatments: untreated (dry in advance), untreated, filler (dry in advance) and filler. Drying in advance was carried for 2 days in dark place and the filler was vermiculite. For storage, in styrofoam box, seed roots were laid down in 3 replicates of 10 sets, stored in – 1℃ warehouse. The storage temperature was set as long as each seed root was not frozen investigating freezing point, supercooling point. As a result, the rate of corrosion was significantly lower in the 4th treatments (filler), such as A. japonica (30%) and A. gigas (10%), compared with untreated one. Therefore, in experiment 2, six treatments were carried out using filler. There were 6 treatments: filler disinfectant (dry in advance), filler disinfectant, filler biochar (dry in advance), filler biochar, filler disinfectant biochar (dry in advance) and filler disinfectant biochar. The disinfectant was used by metalaxyl-M at a concentration of 0.4 g/ℓ, and a biochar was used at a volume ratio of 50% (v/v). As a result, the rate of corrosion was significantly lower in the 6th treatment (filler disinfectant biochar), such as S. miltiorrhiza (13.3%) and C. lanceolata (6.7%). Conclusion : In storing medicinal crop root, it is better to store using fillers. It was shown that a mixture of filler and disinfectant biochar reduced the rate of corrosion.

Background: The present study assessed the response of kenaf (Hibiscus cannabinus L., Jangdae) seed to NaCl and the effects of polyethylene glycol (PEG) on kenaf seed germination and vigor. Methods and Results: Seed germination ranged from 11.3% to 58.8% after 24 hours of immersion in NaCl concentrations from 0% to 0.5%. The priming treatments had lower electrical conductivity (EC) values for the seeds than for the control and a deteriorated palisade layer. Priming in 10% PEG for 48 hours increased the germination upto 96.3% in H2O solution and 98.8% in 0.3% NaCl solution compared to that of the control (78.8%). Germination synchronization, and shoot and root growth of the primed seeds were greater than those of the control. The T50 of the control in H2O and 0.3% NaCl solution was 22 and 28 times, respectively. After priming, nine times was sufficient to reach T50 in both solution. The mean number of days to germination (MDG) decreased from 1.43 days for the control to 0.55 days for 0% PEG in H2O solution and from 1.57 days for the control to 0.56 days for 0% PEG in 0.3% NaCl solution. The dry weight after the 10% PEG treatment was higher than that of the control. Conclusions: Taken together, 10% PEG treatment for 24 hours is recommended for kenaf seed invigoration before planting.