본 연구는 복합추출물(익모초, 어성초, 진피)의 생리활성을 측정하여 식·의약품, 화장품 등의 소재로서의 가능성을 확인하고자 하였다. 복합추출물은 열수 추출하였으며, 세포독성, 항산화, 항염증, MAPKs 등의 평가는 ELISA 및 western blot을 통해 확인하였다. 그 결과, 총 폴리페놀 함량은 35.53±0.48 ㎎ GAE/g, 플라보노이드 함량은 8.18±0.16 ㎎ QE/g으로 나타났으며, DPPH 와 ABTS radical 소거능은 농도 의존적으로 증가하였다. 대조군과 비교하여 세포독성은 400 ㎍/㎖ 농도까지 100% 이상의 생존율이 나타났으며, NO 및 염증성 사이토카인을 유의하게 감소시켰다. 또한, 복합추출 물은 MAPKs(ERK, JNK, p38) 단백질 발현을 유의하게 억제시켰다. 이러한 결과는 복합추출물이 산화 적 손상 및 염증에 사용될 수 있음을 나타낸다. 따라서 본 연구 결과는 식·의약품, 화장품용 복합소재의 의 기초자료로 활용될 수 있을 것이다.

본 연구는 국내에서 재배한 어성초를 각기 다른 조건에서 저장한 후 어성초의 추출액을 이용한 천연 화장품 소재로서의 타당성을 분석하였다. quercetin 의 함량은 건 어성초보다 생 어성초에서 더 높았으며, 총 폴리페놀 함량과 총 플라보노이드 함량을 측정한 결과 건 어성초가 생 어성초에 비해 상대적으로 높은 함량으로 분석되었으나 DPPH 및 ABTS 라디칼 소거 활성은 생 어성초가 상대적으로 높은 결과를 보였다. ROS 소거능과 산화질소 생성 억제율은 생 어성초에서 높은 억제율을 보였으나 100 ㎍/mL의 농도에서 건 어성초가 산화질소를 더 감소시키는 것으로 나타났다. 염증성 사이토카인의 감소를 측정한 결과 IL-1β는 건 어성초가 비교적 높은 감소 효과가 나타났고, IL-6와 TNF-α는 생 어성초에서 높은 감소 효과를 나타냈다. 이상의 결과로부터 생 어성초는 초기 염증 반응에 효과적이며 건 어성초는 염증이 만성 단계로 악화하는 것을 예방하는데 효과적인 것으로 예상된다.

최근 합성첨가물에 대한 소비자들의 거부감이 증가하면서 천연물 유래하는 항산화제, 항균제 등의 개발에 대한 연구가 증가하고 있다. 어성초(Hottuynia cordata)는 흔히 약모밀로 불리는 다년생 초본의 야생약초로서, 항균, 항암, 항바이러스 등의 다양한 생리활성 기능이 있다고 알려져 있으며, 이러한 다양한 효능은 어성초 부위와 추출 방법에 따라 다양한 생리활성물질 때문으로 알려져 왔다. 본 연구에서는 어성초의 잎과 뿌리를 이용하여 열수 추출과 70% 에탄올 추출을 하면서 초음파(ultrasonication) 처리 유무에 따른 추출물의 항산화 및 항균 효과를 비교하였다. 열수 추출에서는 건조된 잎줄기와 뿌리 분말 30 g을 300 mL의 증류수에 넣고, 80℃에서 24시간 추출하여 상등액을 취하고, 다시 같은 양의 증류수로 3회 반복하여 추출하였다. 용매 추출은 각 분말 30 g을 300 mL의 70% 에탄올에 넣고 밀폐시킨 후 40℃ 오븐에서 24시간 추출한 후 상등액을 취하고, 같은 방법으로 3회 반복하여 추출하였다. 열수 추출과 용매 추출 방법에서 초음파 처리에 의한 효과를 비교하기 위해 분말을 증류수 또는 에탄올에 침지 시킨 후 probe type의 초음파기로 20분 동안 처리 한 후 24시간 동안 추출하였다. 추출물은 감압 농축 후 동결건조하여 항산화 및 항균 효과를 평가하였다. ABTS 라디칼 소거활성 결과 잎줄기 추출물이 뿌리 추출물에 비해 항산화 효과가 우수하였고, 열수 추출보다는 에탄올 추출이 효과가 높았으며, 초음파 처리를 병행한 경우 유의적인 증가를 보였다. 잎줄기를 초음파를 병행한 에탄올 추출조건에서 RC50값이 1.09 mg/mL로 가장 낮은 값을 나타내었다.

본 연구는 아토피 피부염을 갖는 질환동물에서 어성초를 비롯한 다양한 식물추출물을 이용하여 제조한 크림의 항아토피 피부염증에 대한 효능을 평가하였다. BALB/c 마우스 를 이용하여 아토피 피부염 유발 대조군 및 크림 처리군으로 완전 임의 배치하였다. 혈액 IgE와 히스타민 농도는 크림 처리군이 DNCB 유도 아토피 대조군에 비해서 유의하게 감소하였으며, 아토피 피부염이 심각한 상태의 마우스에서 정상적인 피부상태로 회복되었음을 관찰하였다. 본 결과는 아토피질환 모델 BALB/c 마우스에서 어성초 혼합 식물추출물의 투여가 혈액 IgE를 낮춤과 동시에 비만세포에서 생성되는 히스타민의 배출량을 억제함으로써 항아토피 효능을 갖는다는 점을 시사 해준다.

Anti-microbiological effects of xanthorrhizol, ie., extract from curcuma xanthorrhiza and extract from houttuynia cordata Thunb. against influenza virus and E. coli O157 were tested. From the influenza experiments, the effects were shown above 93 % in case of houttuynia cordata Thunb. extract, however, the effects was not shown in case of xanthorrhizol. The effects were sustained in mixtures of houttuynia cordata Thunb. and curcuma xanthorrhiza extracts. We also tested the anti-microbiological effects of hand sanitizer containing houttuynia cordata Thunb. and xanthorrhizol. The effect of hand sanitizer containing 2,000 ppm of xanthorrhizol and 500 ppm of houttuynia cordata Thunb. extract was better than that of commercialized foreign product.

환경호르몬으로 독성이 강하기로 유명한 TCDD는 간을 포함한 각 조직에 손상을 주고, 내분비의 붕괴를 야기시킨다. 본 연구는 TCDD로 처리한 rat의 간과 혈장을 사용하여, 어성초의 생화학적 효과를 연구하였다. TCDD를 투여한 지 일주일 후부터 어성초 200 ㎎/㎏을 4주간 격일로 투여하고 TCON군과 NO군은 saline을 투여하였다. 혈청에서 total cholesterol, HDL-cholesterol, LDL-cholesterol, total lipid, triglyceride(7G)를 간조직중에서 MDA를 측정함으로써 과산화지질 활성도와 콜레스테롤의 양을 조사하였다. TCDD로 처치한 rat에 어성초 추출물을 투여했을 때, 과산화지질 및 콜레스테롤이 유의적인 수준으로 억제되었다.

The present study evaluated the blood profile and meat color of ducks with Houttuynia cordata (H. cordata) pellets in their diets. In total, 120 pekin ducks (1-d-old) were used in a 42 d growth trial. Ducks were randomly allocated to six pens and exposed to three replicates of two treatments: (1) controls as basal diet and (2) basal diet + 2% H. cordata pellets (T1). Total-cholesterol, HDL-cholesterol, triglyceride contents or L* and b* vaules revealed significant difference (p>0.05) in compared with those in the controls; however, dietary supplementation of H. cordata pellets did not affect (p>0.05) LDL-cholesterol, VLDL-cholesterol and a* values. In conclusion, the inclusion of 2% H. cordata pellets improved HDL-cholesterol and meat color in duck breast and thigh meats, which further reduced other cholesterol values.

Background : Hair care products are mainly prepared by mixing chemicals and natural extracts, such as those obtained from medicinal plants. The purpose of this study was to investigate the effects of 70% ethanolic extracts from the whole plant of Houttuynia cordata and tymosin β4 powder mixtures on the growth of HaCat cells, hair follicle dermal papilla cells (HFDPC), and 3T3-L1 cells. Methods and Results : In the ratio of tymosin β4 more than 10% concentrations, the cell viability of HaCat and HFDPC cell were increased by higher tymosin β4 concentrations. The mixtures of 70% ethanolic extract of H. cordata and tymosin β4 had no toxicity potential to 3T3-L1 cell viability. In this test, the content of thymosin β4 was higher concentration, as the anti-inflammatory effect was increased. The lipid differentiation of 3T3-L1 cells adipogenesis rate significantly increased in a treatment concentration-dependent manner. Conclusion : These results suggest that a optimal mixture ratio of hair growth effect was 70% ethanolic extract of H. cordata solution 50% and tymosin β4 solution 50%. This mixture solution could be used in the development of hair care products.

The influence of various forms of Houttuynia cordata with fermented red koji as feed additives on the performance of duck livestock was evaluated through a field study. Two hundred and forty ducks (Pekins) aged 0–38 d were used. They were randomly assigned to four treatment groups (0%, 1% H. cordata powder with fermented red koji, 1% H. cordata pelleted with fermented red koji, and 1% H. cordata coated with fermented red koji) with four replicates (15 ducks per replication). Foreconomic evaluation, the data (a total of 240 duck) obtained from each treatment were applied to 10,000 ducks per treatment 38-d after the feeding trial. Overall, the results of economic evaluation were the highest in the treatment with 1% H. cordata coated with fermented red koji, followed by 1% H. cordatapelleted with fermented red koji, 1% H. cordatapowder with fermented red koji, and control. In conclusion, using 1% H. cordatawith fermented red koji as coating and pellet type duck diets might be of economic advantage for sustainable environmental management.

This study made an economic evaluation of duck using Houttuynia cordata (H. cordata) powder as feed additives after 6 weeks. A total of 300 ducks (Pekins) were randomly assigned to one of the three treatments (0%, 0.5% and 1% H. cordata powder), with 5 replicates of 20 ducks each. After 6-week feeding trial, an economic improvement in all the treatments was found in following order: T2 (1% H. cordata powder) > T1 (0.5% H. cordata powder) > control. The results suggested that the addition of H. cordata powder to duck diets provided significantly greater economic benefits, indicating sustainable environmental management of the duck industry.

This study was performed to evaluate the possibility of application of lactic acid bacteria fermentation to increase the anti-allergic activity of the extracts from Houttuynia cordata Thunb. H. cordata Thunb was fermented on 25, 30, 35 and 40℃ for 5 days by two species of lactic acid bacteria, Leuconostoc mesenteroides 4395 and Lactobacillus sakei 383. The anti-allergic activity of water extracts of H. cordata Thunb was then analyzed both before and after fermentation. Anti-allergic activity was determined in vitro assays by using 5-lipoxygenase (5-LO), cyclooxygenase-2 (COX-2) and β-hexoseaminidase release of RBL-2H3 cells (degranulation marker). The extracts fermented at 35℃ by both bacteria had the highest inhibitory activities against 5-LO, and also higher than the control, and the one fermented at 30℃ by both bacteria had the highest inhibitory activity against COX-2. The degranulation of RBL-2H3 cells induced by IgE-antigen complex was estimated as β-hexoseaminidase release rate as reference of 100%, the release rates were inhibited in 25 μg/ml of the extracts fermented at 30, 35 and 40℃ only by L. mesenteroides 4395. These results indicate that anti-allergic activity of H. cordata Thunb is increased by lactic acid bacteria fermentation.

Background: Hair loss related syndromes are increasing due to environmental pollution and stress. Hair care products are mainly prepared by mixing chemicals and natural extracts, such as those obtained from medicinal plants. The purpose of this study was to investigate the effects of 70% ethanol extracts from the flowers of Calendula officinalis, fruit body of Phellinus linteus, and the whole plant of Houttuynia cordata on the growth of CCD-986 cells, hair follicle dermal papilla cells (HFDPC), and 3T3-L1 cells. Methods and Results: All sample extracts at all concentrations, except for that from P. linteus fruit body at 500㎍/㎖, were cytotoxic to CCD-986 cells. However, none of the sample extracts were cytotoxic to HFDPC. The lipid differentiation of 3T3-L1 cells regulates hair regeneration via secretion of platelet derived growth factor. The 70% ethanol extract of H. cordata whole plant promoted hair growth. Adipogenesis rate significantly increased in a treatment concentration-dependent manner. Conclusions: These results suggest that 70% ethanol extracts of C. officinalis flower, P. linteus fruit body and H. cordata could be used for the development of hair care products.

We conducted two experiments to evaluate effects of feeding various types of red ginseng marc and Houttuynia cordata (H.cordata) on blood profiles of poultry in a completely randomized design. In experiment 1 (28 d), a total of 240 broilers (Arbor acres) were used. Four dietary treatments (15 broilers per pen with four replicate pens per treatment) were included: (1) control, (2) 2% red ginseng marc, (3) 1% fermented red ginseng marc with red koji, and (4) 2% liquid red ginseng. There were no significant differences in HDL and LDL-cholestrol among treatments (P > 0.05), but total cholesterol and triglyceride decreased in diets supplemented with red ginseng marc and 1% fermented red ginseng marc with red koji compared to that in the control treatment (P < 0.05). In experiment 2 (38 d), a total of 240 Pekin ducks were randomly divided into 4 groups by dietary treatments (control, 1% fermented H. cordata powder with red koji, 1% fermented H. cordata pelleted with red koji, and 1% fermented H. cordata coated with red koji) with 4 replicates of 15 ducks in each group. Total cholesterol and VLDL-cholesterol were not affected by diet with various types of H. cordata. However, increase in HDL-cholesterol and decrease in LDL and VLDL-cholesterol were greater in treatments with different types of H. cordata than in the control treatments (P < 0.05). In conclusion, using various types of red ginseng marc and H. cordata in poultry diets was effective for increasing HDL-cholesterol and decreasing total, LDL, and VLDL-cholestrol or triglyceride.

Background : Recently, hair loss, which has been regarded as a mere means of middle-aged men due to stress and environmental pollution. The market for hair loss in Korea is about four trillion won and it is growing continuously. It is mainly made by mixing natural extracts such as medicinal plant. The purpose of this study was to investigate the effects of ethanol extracts of Houttuynia cordata Thunb. whole plant and Calendula officinalis L. flower extracts on the growth of fibroblasts, dermal papilla cells and lipid precursors, I want to try to make a materialization. Methods and Results : The cytotoxicity of each sample extracts treated with 50%, 100%, and 500 μg to fibroblasts, cell-viability were 107.3%, 109.6%, and 128.2%, respectively. The cytotoxicity of each sample to the dermal papilla cells was not observed. And the lipid differentiation of the lipogenic precursor cells which regulates the hairegeneration by secretion of the platelet derived growth factor. The 70% ethanol extracts of H. cordata whole plant and C. officinalis flower were showed promotes the hair growth activity. The lipolysis rate was significantly increased with increasing treatment concentration Conclusion : As a result of this study, in-vitro hair growth activity of herbal medicines for hair treatment material development was not shown to be toxic to each cell. And 70% ethanol extract of H. cordata whole plant stimulated lipid precursor cells inducing differentiation. As a result, the 70% ethanol extracts of H. cordata whole plant and C. officinalis flower have potential to developing hair-related product.