Cnidium officinale M. is an important crop that is widely used as a raw material for health functional foods. However, it is experiencing cultivation difficulties due to climate change and abnormally high temperatures. In response to this problem, the characteristics and main causes of the high-temperature damage occurring in C. officinale M. cultivation fields were analyzed. A survey of five farmhouse fields in Jecheon and Bonghwa, major C. officinale M. cultivation areas in Korea in 2018, indicated that about 5% to 37% of the cultivation fields in Jecheon and 5% to 15% of the fields in Bonghwa died from wilting. The high-temperature damage of the C. officinale M. fields is divided into two categories: upper leaves drying due to solar radiation and temperature, and lower leaves dying serially to the radiant heat of the vinyl mulch. Damage caused by radiant heat was typically greater. This is due to the greenhouse effect that occurs in the small space between the black vinyl mulching and the soil. The heat radiated to the surface of the ridge creating an environmental condition that greatly exceeded the atmospheric temperature especially on hot days. As a result, short plants with underground parts, such as C. officinale M., can suffer more high-temperature damage than other plants, so it is considered that it is necessary to develop related technologies such as mulching materials that can reduce pavement temperature in the future.

The purpose of this study was to evaluate the antioxidative activities of jeolpyun containing Cnidium officinale M extract (2%, 4%, 6%, 8%) by total polyphenol contents, electron donating ability on 2,2-diphenyl-1-picrylhydrazyl (DPPH), scavenging ability of superoxide anion radical and decomposing ability of hydrogen peroxide. In chromaticity analysis, the brightness significantly decreased with increasing Cnidium officinale M extract content. Jeolpyun containing 8% Cnidium officinale M extract revealing the highest value for the redness and the yellowness, 1.07, 12.70, respectively. The total polyphenol contents of jeolpyun containing 8% Cnidium officinale M extract were the highest content of 4,213 μg gallic acid equivalent (GAE)/mL. The total polyphenol contents revealed significant difference (p<0.05). Jeolpyun containing 8% Cnidium officinale M extract revealing the highest electron donating ability (83.55%). The electron donating abilities were significantly related at p<0.05. The scavenging abilities of superoxide anion radical for jeolpyun containing 4% Cnidium officinale M extract revealed the highest ability (0.01676). There was no significant difference. The hydrogen peroxide decomposing ability for jeolpyun containing 8% Cnidium officinale M extract revealed the most hydrogen peroxide decomposing ability (－0.193) and the hydrogen peroxide decomposing ability revealed a significant difference (p<0.05).

충북 청주지역에서 채집된 작은소피참진드기(Haemaphysalis longicornis)에 대하여 다양한 식물체 메탄올 조추출물과 천궁유래 물질들을 이용하여 살비활성과 기피활성을 조사하였다. 총 24종의 식물추출물을 이용한 작은소피참진드기에 대한 살비활성을 조사한 결과, 천궁(Cnidium officinale)에서 93.3%의 살비율로 가장 높은 활성을 보였으며, 기피 효과의 경우 도라지, 미국자리공, 백목련, 어성초, 천궁, 칡잎 등에서 통계적으로 유의미한 차이를 보였다. 기피 및 살비활성이 가장 높은 천궁의 메탄올추출물을 용매 분획한 물질들과 천궁 유래물질인 butylidenephthalide 의 활성을 비교한 결과, 헥산(hexane)층 처리 3일 후에 90%의 높은 살비효과를 보였으며 기피반응도 통계적으로 유의미한 차이를 보였다. 천궁 유래물질인 butylidenephthalide은 처리 7일 후에 90%의 살비활성을 보였고 T-tube olfactometer 실험에서 모든 작은소피참진드기가 무처리 구로 이동하여 기피활성이 있음을 알 수 있었다. 따라서 본 연구결과는 천궁 조추출물과 천궁유래물질인 butylidenephthalide를 이용하여 작은 소피참진드기의 방제 개발에 기초자료로 활용될 수 있을 것으로 생각된다.

An analysis of changes in herbal tea composition according to the difference in processing conditions showed slightly reduced crude protein content and increased, moisture, crude fat and solid elution rate after treatment using the ash puffing process compared to roasting. Benzopyrene content was significantly reduced to 0.18 ppb from 0.51 ppb. This result indicated that, the B(α)P content differed depending on the processing condition and raw materials. Generation of food B(α)P is mainly include the thermal decomposition of food cooking, when the processing which is a main component of food carbohydrate, protein, fat reason despite severe heat treatment as a whole is to be detected even though the B(α)P in this way is considered to be. The taste, aroma and color did not show a large difference, but the strong bitters taste decreased.

본 연구는 추출용매에 따른 천궁(Cnidium officinale Makino) 추출물의 생리활성 평가를 통 해 기능성 화장품으로써의 활용 가능 여부를 확인하였다. 추출수율은 에탄올, 열수, 메탄올 추출물 순으 로 나타났다. Xanthine oxidase 저해활성 실험에서는 1,000 ㎍/mL이상의 농도에서 추출용매간의 유의 적 차이가 나타났으며 에탄올 및 메탄올 추출물이 XO 저해활성능이 열수추출물에 비해 상대적으로 높 았다. DPPH radical 소거활성을 분석한 결과 50 ㎍/mL에서 2,000 ㎍/mL으로 농도가 증가함에 따라 에탄올 및 메탄올 추출물은 각각 19.96~89.01% 및 19.41~88.21%로 높은 활성을 나타내었다. 추출용 매에 따른 SOD 유사활성능은 모든 시료에서 농도 의존적으로 증가함을 알 수 있었다. Elastase 및 collagenase 저해 활성능은 추출물 농도가 100 ㎍/mL 이상일 때 에탄올 및 메탄올 추출물의 활성이 유 의적으로 증가함을 알 수 있었다. Tyrosinase 저해활성의 경우 추출농도가 500 ㎍/mL 이상의 경우 에 탄올 및 메탄올 추출물의 경우 유의적으로 tyrosinase 저해활성이 급격하게 증가하였다. 되었다. 이상의 결과로부터 천궁추출물이 미백 및 주름개선의 효과가 있는 것으로 생각되며 향후 기능성 화장품 소재로 서의 이용 가능성을 확인할 수 있었다.

한방차의 roasting 온도를 80∼140℃ 범위로 하여 성분 변화를 분석한 결과, 온도의 상승에 따라 수분함량이 감소하고 일부 탄화가 발생하며 조 회분 함량이 소폭 상승하였고, 조 지방 및 조단백질 함량은 소폭 감소하였다. 한방차의 고형분 용출율은 0.15~0.32%(w/w)를 나타내었는데, roasting 온도가 상승할수록 감소하는 경향을 나타내었다. 처리온도가 80∼110℃구간에서는 큰 변화를 나타내지 않은 반면 110∼140℃구간에서는 고형분 용출율이 급격히 감소하였다. 온도가 상승할수록 용출율이 감소하는 것은 내부 조직이 치밀하여 상대적으로 용출이 어렵기 때문이다. 벤조피렌 함량은 0.29~0.51ppb으로 온도가 높을수록 B(α)P함량이 증가하였다. 처리온도와 원재료에 따라 B(α)P 함량에 차이가 발생하며, 내부온도는 약 200℃정도지만 roaster 표면의 실제 온도는 약 2000℃에 이르는데 표면과 접촉한 부분에서 B(α)P가 생성된다. B(α)P는 주로 음식을 조리, 가공할 때 식품의 주성분인 탄수화물, 단백질, 지방 등이 열분해 되어 생성되는 것으로 생각된다.

팽화 처리온도를 각각 140~220℃로 한 한방차 제품의 성분변화를 분석한 결과는 다음과 같다. 처리온도의 상승에 따라 일부 탄화가 발생하며 조회분 함량이 상대적으로 상승하는 소폭의 변화가 있었고, 조단백질 및 조지방 함량은 거의 변화가 없는 것으로 나타났으며 수분함량은 감소하였다. 한방차의 고형분 용출률은 0.18~0.27%(w/w)로 나타내었는데, 팽화온도가 상승할수록 증가하였다. 고형분의 용출은 온도가 화학적 변화보다 물리적 변화에 의해 식품의 원재료 성분인 탄수화물, 단백질, 지방 등이 천연 상태에서 상호가교 결합이 물리적인 힘으로 어느 정도 파괴되어 성분의 용출이 용이해지기 때문인 것으로 생각된다. 벤조피렌 함량은 0.18~0.24ppb로 처리온도, 원재료에 따라 B(α)P 함량에 차이가 발생한 것으로 나타났다.

This study was performed to investigate the antioxidative effect of Ligusticum chuanxiong Hort extracts (LCE) against the hyperlipidemia of high-fat diet-fed obese rats. The rats were divided into the three groups (normal group, control group and sample group) to perform the experimental research. 1.5 ml/kg of LCE was intraperitoneally administered into the sample group for 21 days. The equal dose of 0.9% saline was intraperitoneally administered into the normal group and the control group. On day 22, they were anesthetized with ether and dissected. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) were examined in serum of rats. Superoxide dismutase (SOD) was measured in mitochondrial fraction. Malondialdehyde (MDA), catalase (CAT), and glutamate peroxidase (GPx) were determined in liver homogenate. High-fat diet markedly increased the levels of AST, ALT and MDA, significantly decreasing those of SOD, CAT and GPx. But Ligusticum chuanxiong Hort-pretreatment decreased the levels of AST, ALT, and MDA. increasing those of SOD, CAT and GPx. These results demonstrated the antioxidative effects, suggesting that LCE could be the candidate for the functional material.

주요 약용작물중(藥用作物中)의 하나로 울릉도와 영양 등 경북 북부지역에서 많이 재배되고 있는 일천궁의 종근생산(種根生産)을 목적으로 경정배양(莖頂培養) 유묘(幼苗)에 있어서 단기간내의 기내발근(器內發根) 배지구명과 포장정식 시기에 따른 지상부 및 근경생육을 알아보기 위하여 시험한 결과를 요약하면 다음과 같다. 경정배양(莖頂培養)을 통해 생산된 유묘의 효과적인 발근배지(發根培地)는 IBA 1.0 mg/L와 BA 0.05 mg/L를 혼용처리한 것이 배양 30일 후 81%가 발근하여 가장 좋았으며 근수(根數)가 9개 이상인 배양묘를 기외이식하고 75% 차광하에 두었을때 생존율이 67%로 높았다. 포장 정식시기를 4월(月) 20일(日), 5월(月) 5일(日), 6월(月) 10일(日), 7월(月) 5일(日)로 하여 정식하였을 때 5월(月) 5일(日) 정식한 유묘(幼苗)가 생존율(生存率) 72%, 초장 33cm, 줄기수 47개로 지상부 생육이 가장 좋았고, 지하부의 근경생육은 5윌 5일 정수록 근경형성율(根莖形成率)은 높은 편이었으나 근경수(根莖數)와 무게는 다소 적었다.

Sprague-Dawley 흰쥐에 고지방식이로 6주간 식이하면서 천궁 열추출액을 경구투여하여 혈장 중 GOT, GPT 및 LDH 활성과 지방의 분해와 합성에 관여하는 코티졸과 인슐린의 함량을 측정하였다. 또한 지방침착억제효과를 광학현미경으로 관찰하였다. GOT, GPT 및 LDH의 활성은 정상군보다 높았으나 대조군보다 낮았으며 현미경 관찰을 통하여 지방간이 약간 개선되고 있음을 나타내어 천궁의 투여가 고지방식이에 의한 비만유발의 개선효과의 경향을 나타내었다.

Sprague-Dawley 흰쥐에 고지방식이로 6주간 식이하면서 천궁 열추출액을 경구투여하여 혈장, 간장 및 부고환조직에서 지방성분, 지단백질의 분포, 변중의 담즙산과 중성스테롤들의 배출량을 측정하여 다음과 같은 결과를 얻었다. 고지방식이와 천궁(CR군)열수추출액을 각각 투여한 흰쥐의 혈청중 총콜레스테롤, 중성지질 및 유리지방산의 농도는 고지방식이로만 사육한 군(CON군)에 비해 낮았다. 한편 인지질과 HDL-cholesterol의 농도는 다소 높았다. 또한 간장과 부고환의 지방성분들을 조사한 경우에도 위와 같은 결과를 얻었다. 고지방식이가 급여되는 동안 정상군보다 대조군에서 low density lipoprotein(LDL)과 very low density lipoprotein(VLDL)이 다같이 증가하고 high density lipoprotein(HDL)의 농도는 감소하였다. 천궁투여군에서는 대조군에 비하여 LDL, VLDL이 감소하였고 HDL은 증가하였다. 그리고 천궁투여군은 대조군에 비하여 분변을 통한 담즙산과 중성스테롤의 배설량이 증가하였다. 이상의 결과를 종합해 보면 천궁투여군이 지방축적 및 고지혈증의 완만한 개선경향을 나타내었다.

양질(良質)의 천궁근(川芎根)을 생산(生産)하기 위한 우량품종(優良品種)을 선발하기 위한 목적(目的)으로 각(各) 주산지(主産地)의 재배산(栽培産) 종근(種根)을 수집하여 종근(種根)의 무게를 표준구(標準區)(20~25 g)와 소구(小區)(15~20 g)로 구분(區分)하여 재배(栽培)한 결과(結果)를 다음과 같이 요약(要約)하였다. 주당(株當) 생근(生根) 및 건조근(乾燥根)의 중량(重量)은 표준구(標準區)가 92.7 g 과 18.5 g 이었으며, 소구(小區)에서는 94.5 g 과 17.0 g 이었다. 지역간(地域間)에는 울릉산(鬱陵産)이 타지역산(他地域産)에 비(比)하여 많았다. 생근(生根) 및 건조근(乾燥根)의 수량(收量)은 표준구(標準區)의 경우 906.8kg/10a와 190.2kg/10a이었으며, 소구(小區)에서는 721.2kg/10a, 165.2kg/10a으로서 표준구(標準區)의 수량(收量)이 더 많았으며, 울릉산(鬱陵産)의 수량(收量)이 타지역산(他地域産)에 비(比)하여 많았다. 회분(灰分) 및 에테르엑기스함량(含量)은 각각(各各) 3.38~3.59%와 3.67~3.87%의 범위에 속(屬)하였으며, 대전(大田), 공주산(公州産)은 회분함량(灰分含量)이, 울릉산(鬱陵産)은 에테르엑기스함량(含量)이 비교적 많았다. 표준구(標準區)에서는 주당(株當) 근중량(根重量) 및 수량(收量)은 에테르엑기스함량(含量)과 고도(高度)의 정(正)의 상관(相關)이, 소구(小區)에서는 회분함량(灰分含量)과 정(正)의 상관(相關)이 인정(認定)되었다. 그러므로 천궁(川芎) 재배시(栽培時)에는 종근(種根)의 무게가 20 g 이상(以上)인 울릉산(鬱陵産)을 선정(選定)함이 수량(收量) 및 품질(品質)이 우수(優秀)한 천궁근(川芎根)을 생산(生産)할 수 있을 것으로 판단(判斷)되었다.

‘중국 전통예술'을 시역（視域）으로 '애니메이션영화 캐릭터 디자인'을 투시한 이 글은 중국의 초기 애니메이션 작품인 ‘대요천궁(大鬧天宮)’을 예로 들며, 구체적인 시공간적 상황 속에서, 전통희곡(傳統戲曲)예술, 회화예술, 전지예술（剪紙藝術）및 민악예술의 관점에서 접점을 만들어 보고, 전형적인 캐릭터를 분석하면서 '민족화（民族化）' 애니메이션 캐릭터의 디자인 핵심을 탐구한다. 또한 애니메이션 영화와 중국전통예술을 결합한 것은 살아있는 전승보호체계를 구축하는 중요한 조치중 하나이며, 산업육성, 홍보등을 통해 전통예술의 생명력과 영향력을 어느 정도 강화할 수 있다.

Background: Cnidium officinale Makino and Ligusticum chuanxiong Hort. are important medicinal crops in Korea. However, there is insufficient information on the identification of thrips, which attack these plants. Until now, one species of thrips has been recorded as a main pest. Methods and Results: To identify the thrips emerging in C. officinale Makino and L. chuanxiong Hort., these plants were independently cultivated in two local areas. Thirty individuals of each plant species were selected randomly and surveyed for the presence of thrips. After confirming the existence of thrips, 100 thrips individuals were collected from each crop using the beating method. To identify thrips species, we performed PCR-restriction fragment length polymorphism (RFLP)-based analysis using ITS2 primer sets. Six thrips species were identified: western flower thrips (Frankliniella occidentalis), flower thrips (F. intonsa), onion thrips (Thrips tabaci), chrysanthemum thrips (T. nigropilosus), chilli thrips (Scirtothrips dorsalis), and grass thrips (Anaphothrips obscurus). The proportion of these species differed between the host plant species. Conclusions: Six thrips species were major pests of two medicinal crops. Integrated pest management is required to control these thrips species, and will enhance the yield and quality of C. officinale and L. chuanxiong.

Background: The leaf temperature (TLEAF) is one of the most important physical parameters governing water and carbon flux, including evapotranspiration, photosynthesis and respiration. Cnidium officinale is one of the important folk medicines for counteracting a variety of diseases, and is particularly used as a traditional medicinal crop in the treatment of female genital inflammatory diseases. In this study, we developed a model to estimate TLeaf of Cnidium officinale Makino based on black globe temperature (TBGT). Methods and Results: This study was performed from April to July 2018 in field characterized by a valley and alluvial fan topography. Databases of TLEAF were curated by infrared thermometry, along with meteorological instruments, including a thermometer, a pyranometer, and an anemometer. Linear regression analysis and Student’s t-test were performed to evaluate the performance of the model and significance of the parameters. The correlation coefficient between observed TLEAF and calculated TBGT obtained using an equation, developed to predict TLEAF based on TBGT was very high (r2 = 0.9500, p < 0.0001). There was a positive relationship between TBGT and solar radiation (r2 = 0.8556, p < 0.0001), but a negative relationship between TBGT and wind speed (r2 = 0.9707, p < 0.0001). These results imply that heat exchange in leaves seems to be mainly controlled by solar radiation and wind speed. The correlation coefficient between actual and estimated TBGT was 0.9710 (p < 0.0001). Conclusions: The developed model can be used to accurately estimate the TLeaf of Cnidium officinale Makino and has the potential to become a practical alternative to assessing cold and heat stress.

Background : Cnidium officinale M. is a medicinal plant used a lot of herbal and functional food ingredients. Recently, the area of cultivation has been increased due to the increase in domestic demand. By the way, this plant is very vulnerable to high temperature, and recently it is difficult to cultivate due to abnormal high temperature and so on. Therefore, in this study, in order to effectively reduce the high temperature of the field, we investigated the degree of temperature reduction and the growth condition of the plant after installed mulching, irrigation and the fog facilities. Methods and Results : In order to reduce the temperature of C. officinale M. cultivation field, the black and white double vinyl mulching, drip irrigation and fog spray treatment were applied and the results were as follows. The survey was conducted at about 2:00 pm on a clear day in August, and the temperature was about 33℃ at the time of measurement. In the case of only black vinyl mulching without watering, the soil temperature was 43.5℃, the surface temperature was 61.4℃, and the mortality rate of the C. officinale M. was 98.0%. The temperature of soil and surface were lowered to 33.1℃ and 38.6℃, respectively, when treated with black vinyl mulching and drip irrigation and fog spraying. In the case of black and white double vinyl mulching, the effect of temperature reduction was better. The soil surface temperature of the non-irrigation treatment was 37.9℃, the surface temperature was 48.5℃ and the mortality rate was decreased to 6.7%. In case of combined drip irrigation and fog spraying, soil temperature was reduced to 31.5℃, surface temperature was 35.8℃, and mortality rate was 0%. Conclusion : As a result of this study, the temperature of soil and surface dropped by up to 12. 0℃ and 25.6℃, respectively, when combined with black and white double vinyl mulching and drip irrigation and fog spray treatment. The mortality rate declined from 89.0% to 0%. Therefore, Therefore, if the results of this study are applied to the cultivation of C. officinale M., it is expected that the production stability will be improved.

Background : Due to changes in climate and cultivation conditions, the growth monitoring is an essential factor in improving crop productivity. With the recent development of image analysis technology incorporating ICT, it has become possible to constantly monitor the crop growth. As a medicinal crop specialized in Gyeongsangbuk-do, Korea, Cnidium officinale Makino was examined for the possibility of growth diagnosis through image analysis for stable production. Methods and Results : The IP camera was installed at 2.5 m height in experiment field. The RGB image of every 06:00 was captured from July 1 to July 30 and used for analysis. The captured images were analyzed using the image analysis tool, Image J. The greeness was estimated by the average value of the green histogram. The canopy size was determined by the color range (red: 0-255, green: mean value-255, blue: 0-255) and was calculated as the ratio of pixels number of the entire image to those of the selected area. The growth temperature during investigation period was measured by Hobo MX2300. High temperature, excess of 28℃, was compared to stress response such as decrement of canopy size. The greeness and the canopy size are respectively represented by the quadratic function greeness = -0.0722GD2 + 6248.9GD – 1e + 08 (GD, growing day; R2 = 0.46) and canopy size = -0.0462GD2 + 3996.7GD – 9e + 07 (R2 = 0.93). From July 11, it began to exceed the growth limit temperature of 28℃, and the canopy size began to decrease from this period. Between the canopy size (C) and the accumulated temperature exceeding 28℃, there was a negative correlation, C = -0.13ATEC + 56.75 (R2 = 0.87) during the decreasing period. Conclusion : Extraction of color information in Cnidium officinale Makino using RGB image should be preceded by standardized setting, but it is considered to be useful tool for analyzing the change of quantitative characteristics over time. In the future, it is necessary to make a comparative study with the actual growth rate in the image diagnosis.

Background : Two major forest medicinal resources, Korean angelica (Angelica gigas Nakai) and Cnidii rhizoma (Cnidium officnale Makino) were used as medicinal herbs for long times and in these day consumed as cosmetic materials. For keep pace with a mass demand and supply, pesticides were should be sprayed for control of pests and plant diseases. However, the social concerns on well-balanced life, pesticides safety, and continuous and stable production of clean non-timber products were increased with the times. Therefore, environmental-friendly cultivation was increased before decade. This study performed for selection of effective control agents to hyphal growth of leaf spot and anthracnose in two medicinal resources. Methods and Results : The antifungal effects of eugneol, pine needle E.O, and 7 natural product extracts (Sophora root, green tea, neem, eucalyptus, grapefruit seed, derris, and 36% rosin) were evaluated against two plant pathogens (Phoma sp., and Colletotrichum gloeosporioides). After re-loading of each 20 ㎕ natural product extracts to the PDA plate which loaded two spore dilution (106 spores/㎖) 100 ㎕, the hyphal growth control effects to pathogens were confirmed. Among 9 control agents, eugenol and pine needle E.O were the most effective, and then Sophora root, green tea, and neem. However, 4 agents (eucalyptus, 36% rosin, grapefruit seed, and derris) were not effective. Conclusion : It confirmed that possibility as a control agents of eugenol and pine needle E.O to leaf spot (Phoma sp.) of Korean angelica and anthracnose (Colletotrichum gloeosporioides) of Cnidii rhizoma, respectively. We will be developed as commercial products safe in the forest lands