European and Turkish rice varieties were analysed to identify the traits associated with low-temperature germination. The aim of the study is to develop new rice varieties that can use these traits to reduce greenhouse gas emissions in rice fields. The average low temperature germination ratio (GR) in the European and Turkish rice varieties was 89.0±14.1%. The speed of the germination rate (SG) in Korean early maturing varieties, ‘Jopyeong’ and ‘Baekilmi’ was 1.3 and 3.5, respectively, whereas the European and Turkish varieties had a SG of 6.6. In terms of germination energy (GE) by the date, the European and Turkish varieties started germination within 4 days, while ‘Jopyeong’ and ‘Baekilmi’ began to germinate after 8 and 10 days, respectively. The mean germination time (MGT) for the European and Turkish varieties was 9.9±1.2 days. Between 4 to 10 days after inoculation, the germination velocity coefficient (GVC) for the European and Turkish varieties increased moderately from 2.6 to 5.3. After 10 days, the GVC rose rapidly from 7.0 to 12.0. ‘Jopyeong’ and ‘Baekilmi’ had slower germination rates compared to the European and Turkish varieties, in which the GVC increased moderately to 3.2 and 2.3, respectively, between 7 and 9 days after inoculation. The average mesocotyl elongation ability was 4.0±0.4 cm, with a range from 1.3 cm to 7.3 cm.

The objective of this study was to determine the changes in content of polyphenolics, flavonoids, amino acids, especially γ-aminobutyric acid (GABA), and the antioxidant activities in the methanol extracts of four different pigmented rice seeds (white, black, red, and green color) at different germination stages, dry seeds (DS), imbibed seeds (IS), and germinated seeds for 1 (GS1), 2 (GS2) and 3 days (GS3). Total phenolics content at 1000 mg/kg extract was highest in black rice, ranging from 208.3 to 257.3 mg/kg, and followed by red rice 144.7 to 185.7 mg/kg, brown rice 151.3 to 194.2 mg/kg and green rice 125.7 to 160.7 mg/kg (lowest). Total flavonoid level had same tendency to total phenolics among colored rices with significant differences, showing highest amounts (45.2 to 56.8 mg/kg) in black rice and lowest (18.8 to 23.0 mg/kg) in green rice. Total phenolics and total flavonoids levels in black and brown seeds were highest when soaked, and in red rice when nonsoaked, and in green rice when germinated for 1 day. These results showed that content of physiologically- active substances were differ depending on seed coat color, soaking or germinating degree. Content in total amino acids during germination was ranged from 136.5 to 232.0 mg/kg in green rice (highest), and 98.9 to 176.0 mg/kg in red rice (lowest). Total content of amino acids and GABA level in all seed colors were high in order of GS3 > GS1 > IS > DS. The antioxidant activity of the methanol extracts from all the seed colors concentration-dependently increased. DPPH free radical scavenging activity at 2,000 mg/kg was highest in black rice by 83.0 to 87.7%, and lowest in green rice by 44.3 to 65.7%.

직파품종 개발을 위한 특성검정으로 저온발아성을 조사하여 직파적응성에 알맞은 품종개발을 위한 기초자료로 활용하고자1994년부터 2008년까지 육성된 89개 품종을 이용하여 저온발아성을 검정한 결과 최근에 육성한 품종일수록 저온발아율이80% 이상으로 높았으며, 조생종이나 중생종보다는 중만생종의 저온발아율이 높은 경향이었으나 통계적인 유의차는 인정되지 않았다. 또한 최고품질이나 고품질 품종이 초다수성이나안전성 품종보다 저온발아율이 높았다. 품종이 육성된 기관별로 볼때는 직파품종 개발을 담당하고 있는 익산의 벼맥류부육성품종의 저온발아율이 다른 기관에서 육성된 품종보다 높게 조사되었다.

일반적으로 현미를 발아시킬 경우 다양한 효소의 활성화로 인하여 GABA, ferulic acid, arabinoxylan, inositol 등의 기능성 성분들이 증가될 뿐만 아니라 조직을 연화시켜 현미의 식미를 개선시키는 것으로 알려져 있다. GABA는 발아현미에 존재하는 기능성 물질 중의 하나로 비단백질 아미노산이다. 본 연구의 목적은 발아 후 품종별 GABA 함량의 변화를 측정하고자 하였으며 이와 함께 vitamin E의 변화와 항산화력의 변화를 측정하고자 하였다. GABA 함량은 비색법을 이용하였고 vitamin E는 HPLC 방법을 항산화력은 ABTS와 DPPH 라디칼 소거능을 이용하여 측정하였다. 본 연구 결과 품종별 현미는 발아 과정을 통하여 GABA 함량은 약 10배 이상 그리고 vitamin E의 경우 tocotrienol이 상당히 증가하였으며 항산화 활성에는 전반적으로 변화가 없는 것으로 나타났다. 특히 큰눈 발아 현미의 경우 GABA 함량이 다른 품종에 비해 월등히 높았고 vitamin E 함량과 항산화 활성은 신명흑찰 발아현미가 높은 값을 나타내었다. 본 연구결과 현미를 발아시키면 GABA 및 vitamin E 등과 같은 생리활성 물질이 증가되고 항산화 활성이 그대로 유지된 기능성 쌀 가공품의 원료를 얻을 수 있을 것으로 생각한다.

벼의 유묘 생육과 광합성 효율에 대한 저선량 방사선과 생장조절물질 간의 상승작용 효과를 확인하기 위해 4년 된 종자로 기내실험과 온실실험을 수행하였다. 기내실험에서 관찰한 종자 발아의 경우 방사선을 조사한 IBA 0.001 ppm 처리구에서 가장 높은 값을 보였다. 또한, 파종 6일 후 측정한 유묘 생육은 방사선 무 조사구보다 방사선을 조사한 GA₃와 IBA 처리구에서 대체로 높은 값을 보였으며, 그 중 IBA 0.001 ppm 처리에서 저선량 방사선과

The germination test was used to detect biological changes in irradiated rice and glutenous rice at low doses. Grains were irradiated at below 0.5 kGy, husked and placed on distilled water moistend filter paper in a covered petri-dish. A germination test of 20 grains was carried out at room temperature for 5 days. The shoots and roots of non-irradiated rites grew well in comparison with those of irradiated rites above 0.3 kGy. The roots of rites were more sensitive to irradiation than the shoots, and the growth of roots was significantly decreased with the increasing doses. In glutinous rites, the growth of shoots and roots was retarded by irradiation at 0.2 kGy or more after 3rd days. We concluded that if the shoot or root length is 10 mm or longer within 5 day, the rites and glutinous rites are identified as non-irradiated.

1. 시험은 1961과 1962년의 2개년에 걸쳐 대관령에서 미국, 일본, 독일계와 한국재래품종 53품종을 공시하여 감자역병에 대한 저항성을 조사할 것이다. 2. 본시험은 자연발병하에서 조사하였으며 항저성의 차이를 다섯 계급으로 구분하였는데 그 결과는 아래와 같다. 면역성품종...Kennebec, Isola, Cherokee, Merrimack, Yoraku, Lisili, Cosima. 고도저항성품종...Antze, Ragis Isola, Delus, Hessenkrone, Plymouth, Sebago, Pungo, Sieglinde, Anco, Essex. 저항성품종...Tawa, B-605-10, Sequoia, Grata, Concordia, Onaway, Noring 1, Nisego. 이병성품종...Nordak, Pontiac, Benimaru, Early gem, Chippewa, Chitose, Ohjiro, Red Bake, Norland, Katahdin. 고도이병성품종...Russet Burbunk, May Queen, Earlaine, Irish Cobbler, Feldeslohn, Red Warba, Chisago, Osseo, Paunee, Warba, Norgleam, Red Beauty, Red, Danshaku, Namchak(Native variety). 3. 만생종품종이 조생종보다 저항성이었다. 4. 일본계와 미국계도입품종보자 독일계도입품종이 저항성이 강한 편이었다.

‘Nokwoo’, a mid-late maturing, high dry matter yielding rice (Oryza sativa L.) cultivar with a good early growth and low-temperature germinability, was developed for whole crop silage (WCS) use. It was derived from a cross between a leafy tropical japonica ‘LK1A-2-12-1-1’ with high biomass and good germinability in low temperature and new plant type (NPT), ‘IR72225-29-1-1’ which had low tillering trait, large panicle, dark green leaf, thick and sturdy stem and vigorous root system. This cultivar had about 123 days growth period from seeding to heading, 122㎝ culm length, 29㎝ panicle length, 9 panicles per hill, 144 spikelets per panicle and 1,000-grain weight of 24.4 g as brown rice in central plain region, Suwon. This wide and long leafy WCS rice variety was weak to cold stresses similar to ‘Nokyang’ but was a little resistant to lodging in the field, strong to viviparous germination and good to low temperature germination. In addition, ‘Nokwoo’ was resistant to leaf and neck blast but susceptible to bacterial blight, rice stripe virus and brown planthopper. Its average dry matter yield for three years reached 16.5 MT/ha, 14% higher than that of ‘Nokyang’. This cultivar had 5.3% crude protein and 68.8% total digestible nutrients a little low compared to ‘Nokyang’. In Korea peninsular, ‘Nokwoo’ grows well in central and southern plain and is good to harvest between 15 to 30 days after heading to improve its feeding value and digestion rate of livestock (Grant No. 6072).

Germination characteristics, seedling emergence, and early seedling growth of iron-coated rice seeds, cultivars Daebo and Samdeokbyeo, under different water depths were compared with those of non-coated seeds (control) and the results evaluated to obtain basic information for establishing stable seedlings in direct water seeding. The total germination percentage of the two seed treatments was similar, but iron-coated seeds had slightly faster germination and shorter mean germination time than non-coated seeds. Water absorption rates of iron-coated seeds were lower than that of non-coated seeds during seed germination. The germination percentage of the two iron-coated rice seed cultivars showed a significant decline of 15-22% after one year of storage under natural conditions. The seedling emergence percentage and uniformity of the two rice cultivars were significantly higher in the iron-coated seeds at 1-13 cm water depths but the percentage of floating seedlings was lower in iron-coated seeds than in non-coated seeds. The iron-coated seeds had a high seedling emergence percentage of 91.3-93.3% at all flooding depths whereas the non-coated seeds had a significantly low seedling emergence percentage of 57.7-71.7% at a water depth of 13 cm. Moreover, the shoot dry weight and seedling health score of iron-coated seeds were significantly higher than those of non-coated seeds, while root dry weights were similar in iron-coated and non-coated seeds, regardless of water depth. These results suggest that iron-coated seeds are more appropriate for stable seedling establishment in direct water seeding than are non-coated seeds.

Although rice has been cultivated as a major food crop for approximately 5,000 years, the interest of customers in ‘scented rice’ is a recent trend in the Korean market. As a part of developing a germinated scented rice variety, the newly bred scented rice variety ‘Cheonjihyang-1 se’ was germinated for 24 h, and changes in profiles of flavor-related volatiles, lipophilic phytonutrients, and fatty acids were investigated. The profiling of volatile compounds by using a headspace-gas chromatography-mass spectrometry (HS-GC-MS) revealed a total of 56 odor-active flavoring compounds; 52 at the pre-germination stage, 51 at the post-germination stage, and 47 common at both stages. The major flavoring compounds were nonanol and benzene, which constituted 11.5% and 6.6%, respectively, of the total peak area in pre-germinated rice, and 19.4% and 6.5%, respectively, in post-germinated rice. Germination induced an increase in 13 flavoring compounds, including 3,3,5-trimethylheptane and 1-pentadecene, which increased by 763 and 513%, respectively by germination. However, we observed a germination-induced decrease in most of the other flavoring compounds. Especially, the most important scented rice-specific popcorn-flavoring compound, 2-acetyl-1-pyrroline, showed 89% decrease due to germination. Furthermore, the germination of scented rice induced a decrease in the content of various phytonutrients. For example, the total contents of phytosterols, squalene, and tocols decreased from 207.97, 31.74, and 25.32 μg g -1 at pre-germination stage down to 136.66, 25.12, and 17.76 μg g -1 , respectively at post-germination stage. The fatty acid compositions were also affected by germination. The composition of three major fatty acids, linoleic, oleic, and palmitic acids, increased from 36.6, 34.2, and 24.4%, respectively, at the pre-germination stage to 37.9, 36.9, and 20.7%, respectively, at the post-germination stage. All these results suggested significant changes in the flavor-related compounds and phytonutrients of the scented rice variety ‘Cheonjihyang-1 se’ during the process of germination, and subsequently the need for developing a more precise process of germination to enhance the flavor and nutritional quality of the germinated scented rice products.

The objective of this study was to evaluate quality properties, including amylopectin content, and texture analysis of brown rice and germinated brown rice cultivars in Korea for rice processing products. The amylopectin short chain content of germinated brown rice was significantly higher than that of brown rice. Texture analysis test showed that germinated brown rice Jinbo had the lowest hardness and toughness as well as and highest stickiness and adhesiveness. The correlation between degree of polymerization of amylopectin and texture analysis was also evaluated. In particular, germinated brown rice, short-chain amylopectin showed a negative correlation with hardness and toughness, whereas long-chain amylopectin showed a positive correlation with hardness, toughness, and adhesiveness. These results indicate that there is a relationship between degree of polymerization and texture analysis.

Seeds of colored rice generally have high anthocyanin and flavonoid content but some cultivars have low seed germination rate. This defect poses unstable seedling establishment in nursery bed. The seed gravity variations of 10 colored rice varieties and its effect on germination, seed soaking duration and their optimum seeding rates for seedling raising in machine transplanting were investigated in the experiment. Based on seed gravity distribution of the 7 black- and 3 red-colored rice varieties at three levels of seed gravity (1.0<, 1.0~1.06, 1.06>), the black colored seeds such as Josaengheugchal and Sinmyungheugchal had high amount of specific gravity of below 1.0 ranging from 86~ 96%, while they had only 3~13% of seeds in specific gravity above 1.06. Sintoheugmi, Heugjinju, Heugnam, Heugkwang had various mass of seeds, showing 29~44% for specific gravity of below 1.0, 24~39% for 1.0~1.06 and 25~45% for above 1.06. On the other hand, the red colored rice such as Hongjinju, Jeogjinju and Geonkanghongmi had high percentage of specific gravity of above 1.06 with 84~86% while they had only 9~12% of specific gravity below 1.0 similar to Ilmibyeo of noncolored rice. The black colored seed generally showed low germination percentage, slow germination speed and long mean germination time, and low water absorption rate as compared with seed of the red colored rice which was similar to those of Ilmibyeo used as a control cultivar. The black colored seeds took 2~4 days longer seed soaking duration than the red colored rice before germination. This was related to high seed amount of specific gravity below 1.0 in black colored rices. The high amount of seed gravity above 1.06 in the colored rice seeds was positively correlated with percentage of germination, germination speed and mean germination time, ripened grain ratio and water absorption. Seed gravity distribution effect were less pronounced between back- and red- colored seeds in seedling emergence. The black colored rice has slightly lower normal seedling emergence rate than the red colored rice and Ilmibyeo in the seedbed soil due to high percentage of ungerminated seed. Normal seedling emergence rate of the black colored rice in the seedbed was 75.2~82.2% for 10-day old seedling and 85.3~ 90.9% for 30-day old seedling which was lower by 4.5~ 8.0% and 0~3.3%, respectively, than the red colored rice. Based on the normal seedling number per tray of Ilmibyeo for seeding rate of 10-day and 30-day old seedlings, the recommended seeding rate of black colored rice is 200~ 220g seeds for the 10-day old seedling and 110~130g for the 30-day old seedling in transplanting rice while the seeding rate of the red colored rice was 220g seeds for the 10-day old seedling and 130g for the 30-day old seedling.

1. 생산지에 따라 벼 종자의 수발아립률 차이가 컸던 호 품벼의 출아율과 발아율을 조사한 결과, 수발아율 8% 인 시료는 수발아율이 1%인 시료에 비해 발아율과 출 아율, 종자활력이 30% 낮았다. 2. 수발아율이 높은 시료는 수발아립의 변색 정도가 심 하고 미발아율과 부패율이 높았다. 수발아립은 배가 돌출하고, 변색된 배유와 곰팡이 감염이 관찰되었으며 배유의 1/2이상이 변색된 종자는 발아율이 매우 낮았다. 3. 종자의 발아 진행정도에 따라 현미의 변색이 심해졌 는데, 싹이 3 mm이상일 경우 배유의 1/2정도가 변색 되었다. 4. 발아 종자는 싹이 3 mm이상일 때부터 재발아율이 크 게 떨어졌으며 퇴화촉진처리 후 발아율의 감소가 더 컸다. 특히 퇴화촉진처리한 발아 종자는 싹이 2 mm부 터 종자활력이 크게 감소하였다. 결론적으로 수발아 발생이 심할수록 파종기 발아율과 출 아율의 감소가 크고, 수발아가 3 mm이상 발생하면 발아율 이 크게 저하될 것으로 생각된다. 또한 수발아는 동일 품종 과 동일 지역에서도 포장 간 차이가 크게 발생할 수 있으므 로 재배관리에 주의해야 한다.

Rice cultivation by direct seeding allows reduced labour and production costs in addition to other benefits. However the success of this rice production method can be limited by uneven fields with poor drainage or heavy rainfall at sowing leading to early flooding conditions slowing germination and hindering crop establishment. Hence, the need to improve tolerance to anaerobic conditions in both rainfed and irrigated rice ecosystem after direct seeding. In this study QTL analysis was performed to identify QTLs associated with tolerance derived from Vietnamese variety Tai Nguyen (TN) under anaerobic conditions during germination. The population derived from a cross between TN (tolerance indica lines) and Anda (susceptible japonica), was used for collection of phenotypic data based on the survival rates of the seedlings at 21 days after sowing under 10cm of water. A total of 286 F2:3 families of the population were used for QTL mapping and the genotyping was carried out with the infinium 6K SNP-chip based on the illumina infinium platform. Two significant QTLs associated with the AG trait were detected on chromosomes 1 and 11, respectively. In Particular, the QTL on chromsome 1 had an LOD score of 7.45 and R2 of 14.21%. We plan to confirm the identified QTLs in further studies and develop varieties with improved anaerobic germination ability using advanced backcross lines.

Anaerobic germination (AG) is plays important role in submergence resistance which is an important trait for rice production in flood-prone lowland areas. Slow seed germination and delayed seedling establishment due to flooding become major problem for modern sowing methods such as direct seeding and environmental friendly good agricultural practices especially using young seedling age. In total, 137 diverse rice accessions were evaluated for anerobic germination ability. Submergence 1 (SUB1) which is induced ethylene response factors is suggestive because genes belonging to this gene family play a crucial role in rice tolerance to submergence. In this study, haplotype variations of three AG related genes, SUB1 (SUB1A, SUB1B, SUB1C) were examined using whole-genome resequencing data of 137 accessions of rice core set. The new SNPs and InDels found in the exon of the sub1 loci would be useful in developing markers to screen the varieties with strong anaerobic germination ability in the future molecular breeding

Salt toxicity is the major factor limiting crop productivity in saline soils. Rice is an important staple food crop of nearly half of the world population and is well known to be a salt sensitive crop. The completion and enhanced annotations of rice genome sequence has provided the opportunity to study functional genomics of rice. With the rapid development of the biotechnology techniques, we can use more accurate and reliable methods to study the mechanism and function in different stress conditions. In present study, 295 rice accessions of diverse origin were re-sequenced and used for genome-wide association study (GWAS) with several germination-related traits, including germination percentage (GP), germination energy (GE), germination rate (GR), germination index (GI), salt tolerance index (STI) in salt tolerant germination stage. Phenotyping of the rice accessions were carried out at 200mM NaCl to screen salt tolerance levels. GWAS was applied to detect the associated genes related to salt tolerance in rice germination stage. Variations and haplotypes of the associated genes were detected and correlation between the phenotypes and genotypes were validated using qRT-PCR.

찰벼 4품종을 두 온도조건(저온; 15℃, 대조; 25℃)에서 왕겨의 유무에 따라(정조 및 현미) 발아특성을 조사한 결과, 현미 발아율은 품종간 차이가 없었으나 정조 발아율은 저온 에서 품종간 차이가 컸다. 종자를 침윤시키면 온도나 왕겨 의 유무에 관계없이 수분이 급격히 증가하다가 30%에서 더 이상 증가하지 않고 정체하는데 이때 정체기에 도달하는 시 간은 온도가 높을수록, 정조보다 현미가 빨랐다. 그러나 수 분정체기에 도달하는 시간은 품종간 차이가 있고 저온에서 차이가 컸다. 수분흡수시간은 발아율, 발아세, 발아속도, 평 균발아일수와 모두 높은 부의 상관을 보여 초기 수분흡수시 간이 빠를수록 발아를 촉진하는 것으로 나타났다. 침윤 24 시간 뒤 guaiacol peroxidase활성은 처리 및 품종간에 큰 차 이를 나타냈으며 발아세와 발아속도와 정의 상관이 있었으 나 최종발아율과는 상관이 낮았다. Catalase활성은 발아율, 발아속도 등과 상관이 매우 낮았으며, 용해성 단백질함량 및 maltose농도도 발아특성과 상관이 낮았다. 종자활력검사 (TTC활력검사)와 표준발아율검사(25℃)에서 발아율이 높 더라도 정조는 저온에서 품종간 발아율의 변이가 커 종자활력검사만으로 발아율을 예측하기 힘들었다. 따라서 초기의 종자활력은 TTC검사와 guaiacol peroxidase활성을 함께 고 려하는 것이 더 정확한 것으로 생각된다. 또 품종간 발아율 의 변이를 줄이고 발아속도를 높혀 균일한 발아를 유도하기 위해서는 적정범위에서 가능한 온도를 높혀 수분흡수를 촉 진시켜야 한다.