고춧가루의 품질 변화를 낮추면서 살균하는 기술을 개발하고자 감압방전플라즈마(LPDP)를 이용한 비가열살균을 시도하였고 LPDP 처리에 의한 품질변화를 조사하였다. 시중 판매 고춧가루의 오염도는 세균 7.33×104-1.07×107 CFU/g, 진균류 3.47×104-3.16×106 CFU/g의 범위를 보여 상당한 수준으로 오염된 식품으로 확인되었다. 고춧가루의 LPDP 살균 패턴은 2단계 1차 반응으로 나타났으며, 출력에 따라 살균속도상수가 증가하였다. 또한 입자가 가늘수록 살균효과가 증가하였으며, 미생물 종류별로는 세균에 비하여 진균에 대한 살균효과가 크게 나타났다. 과도한 LPDP 처리는 고춧가루의 탈색과 capsaicinoids 함량의 감소를 유발하였다. LPDP 처리 고춧가루를 사용하여 조리한 무채의 관능검사 결과 색과 맛은 5분 처리까지는 무처리구와 차이가 없었으나 10분 처리 시에는 유의하게 낮은 점수를 보였다. 반면 향과 기호도에서는 처리구와 무처리구 간에 유의차를 보이지 않아 5분 이하로 LPDP 처리 시 관능특성을 유지하면서 약 1.5 log 정도의 미생물 저감화가 가능하였다.

본 연구는 두부의 침지수에 AC를 처리하고 저장기간에 따른 품질의 변화를 측정하여 두부의 저장안정성을 조사하고자 수행하였다. 두부 침지액으로는 증류수, 0.05% AC 용액, 0.1% AC 용액 및 0.15% AC 용액을 사용하여 10oC에 저장하면서 pH와 탁도, 색도, 관능검사, 그리고 미생물의 변화를 관찰하였다. 침지액에 AC를 처리한 두부는 대조구와 비교해 보았을 때 pH가 높게 나타났고 저장기간이 늘어남에 따라 두부의 pH가 약간 감소하였다. 대조구에서 탁도는 두부의 저장기간이 늘어남에 따라 급격히 증가하였으나 AC를 첨가한 두부에서는 농도 의존적으로 탁도의 변화가 감소하는 경향을 보였다. 색도를 측정한 결과 AC를 처리한 두부에서 모든 군에서 차이를 나타내지 않았다. 두부의 관능검사에 있어서 저장기간 동안에 AC를 처리한 두부는 관능적 특성을 더 오래 유지하였으나 AC의 첨가율이 높을수록 색에 대한 기호도가 떨어지고 맛에도 영향을 미쳐 전체적 기호도를 저하시켰다. 두부의 씹힘성과 경도는 AC의 첨가율이 높을수록 낮아졌으나 이물감 조성에는 영향을 끼치지 않는 것으로 나타났다. Texture특성을 관능검사를 통해 측정한 결과 경도, 탄성, 검성, 씹힘성은 0.1% AC와 0.15% AC 용액을 침지액으로 사용하였을 때 대조구와 비교해서 낮은 수치를 나타내는 것을 볼 수 있었다. 두부의 저장기간 동안 호기성 세균수는 대조구에서 7일 후 6.00 log CFU/g을 초과하였으나 0.1% AC와 0.15% AC 처리 시에는 미생물의 증식이 효과적으로 억제되어 저장기간이 연장되는 결과를 보였다. 이와 같은 결과로 볼 때, AC의 침지처리는 10oC에서 7일 동안 가공두부의 선도유지 기간을 연장할 수 있는 효과를 기대할 수 있었다.

자기 제조공정 중 고온에서 형성된 클레이 나노입자를 충진제로 하여 LDPE-클레이 나노컴포지트 시트를 제조하였으며, 이의 산소, 이산화탄소 및 수분에 대한 투과도를 서로 다른 세 온도(20, 30 및 40oC)에서 측정하였다. 사용한 클레이 입자의 주성분은 ICP-OES를 통하여 Ca(40.733%)임을 확인하였으며, 나노컴포지트 내 클레이 나노입자가 잘 분산되어있음을 TEM을 통해 확인하였고, FT-IR을 통하여 클레이 나노입자의 첨가가 LDPE의 작용기에 특별한 영향을 주지 않았다는 것을 확인하였다. 산소, 이산화탄소 및 수분투과도는 예상하였던 바와 같이 온도가 상승함에 따라 증가하는 경향을 보여주었다. 클레이 나노입자의 혼입으로 시트 내 기체분자의 확산 경로가 증가됨에 따라 모든 테스트 온도에서 나노컴포지트 시트는 대조구보다 유의적으로 향상된 기체차단효과를 나타내었는데, 산소와 이산화탄소에 대해서는 30 및 40oC에서 보다 20oC에서 상대적으로 강한 차단성을 나타내었으며, 수분에 대해서는 30oC에서 차단성이 상대적으로 높았고 20 및 40oC에서는 비슷한 차단효과를 나타내었다. 본 연구결과를 통하여 나노입자를 혼입 함으로서 LDPE 시트의 산소, 이산화탄소 및 수분에 대한 차단효과를 향상시킬 수 있음을 확인하였으며, 동시에 이러한 차단효과 향상의 정도는 온도에 따라 상당히 차이가 있음을 알 수 있었다. 차단효과의 온도 의존성에 대한 구체적인 이유는 아직 밝혀지지 않았지만, 온도에 따라 기체확산 및 고분자운동의 정도가 달라지므로 나노입자에 의한 기체확산 경로 증가의 효과 역시 변하게 되기 때문으로 사료된다. 따라서 나노컴포지트를 포장재로 사용할 경우, 반드시 목적온도에서 기체차단효과를 확인한 후 응용하여야 할 것으로 사료된다. 또한 시트나 필름의 내부 고분자 배열은 그 형성방법에 따라 달라지므로, 이것이 나노컴포지트의 기체차단효과에 미치는 영향 역시 신중히 고려되어야 한다. 이러한 현상에 대한 충분한 이해를 위해서는 나노입자 및 고분자의 배열구조와 기체차단효과의 상관관계에 대한 연구가 필요할 것으로 사료된다.

사출구의 기하학적 구조와 탄산가스 주입량이 압출성형물의 물리적 특성에 미치는 영향을 분석하기 위해 사출구의 기하학적 구조를 사출구상수로 산출하여 사출구상수와 수분함량, 용융물의 온도, 탄산가스 주입량에 따른 압출성형물의 물리적 특성을 분석하였다. 압출성형 공정변수는 수분함량 30%, 스크루 회전속도 150 rpm, 원료 투입량 100 g/min, 용융물의 온도(95, 110oC), 탄산가스 주입량(0, 100, 200 mL/min), 사출구 길이(2, 5 mm), 내벽에서 좁아지는 각(57, 95o)이었다. 비기계적 에너지 투입량은 사출구 상수 3.36×10-10 m3, 탄산가스 주입량 200 mL/min, 용융물의 온도가 110oC 일 때 159.31 kJ/kg으로 가장 낮은 비기계적 에너지 투입량을 나타내었다. 또한 직경팽화율은 내벽에서 좁아지는 각이 57o(3.36×10-10, 6.82×10-10 m3)일 때 95o(3.64×10-10, 8.09×10-10 m3)보다 높은 경향을 보였으며, 탄산가스 주입량이 0 mL/min에서 200 mL/min으로 증가함에 따라 직경팽화율은 증가하였다. 탄산가스 주입량이 증가할수록 비길이는 증가하는 경향을 나타냈으며, 사출구 상수가 낮을 때 밀도와 파괴력, 겉보기 탄성계수는 낮은 경향을 나타내었다. 압출성형물의 미세구조를 관찰하였을 때 용융물의 온도 110oC에서 내벽에서 좁아지는 각이 95o(3.64×10-10, 8.09×10-10 m3)일 때 57o(3.36×10-10, 6.82×10-10 m3)보다 기공의 수가 많았다.

세계화 및 기후변화 등으로 외래병해충의 유입가능성은 크게 증가되고 있어 미 국, 호주 등 선진국의 경우 검역병해충에 대한 첨단의 진단기술개발 이외에 외래침 입병해충으로 인한 경제적 영향분석, 긴급대응체계 구축, 사후관리 등 식물검역 R&D의 중요성을 인식하여 투자를 대폭 증대하고 있다. 우리도 이러한 국제적인 추세에 맞춰 2011년 6월 농림수산식품부 산하 검역관 련 3개 기관이 통합하여 농림수산검역검사본부로 출범하였고, 식물검역 R&D를 활성화하기 위해 식물검역기술개발센터가 신설되었다. 이 센터가 조기에 목적을 달성할 수 있도록 2013년부터 식물검역 R&D분야의 투자영역을 사전예방체계 확 립, 첨단융복합 진단체계 구축, 친환경 소독기법 개발, 확산방지 및 사후관리기술 개발, 식물검역 선진화기반 강화로 설정하고, 내실있고 균형있는 투자를 통해 식물 검역 R&D의 기반을 구축해 나가고자 한다.

We compared between an automated most-probable-number technique TEMPO®TVC and traditional plating methods PetrifilmTM for estimating populations of total aerobic bacteria in various livestock products. 257 samples randomly selected in local retail stores and 87 samples inoculated with E. coli ATCC 25922, Staphylococcus aureus ATCC 12868 were tested in this study. The degree of agreement was estimated according to the CCFRA (Campden and Chorleywood Food Research Association Group) Guideline 29 and the agreement indicates the difference of two kinds methods is lower than 1 log base 10(log10). The samples of hams, jerky products, ground meat products, milks, ice creams, infant formulas, and egg heat formed products were showed above 95% in the agreement of methods. In contrast, proportion of agreement on meat extract products, cheeses and sausages were 93.1%, 92.1%, 89.1%, respectively. One press ham and five sausages containing spice and seasoning, two pork cutlets containing spice and bread crumbs, two meat extract product and two natural cheeses and one processing cheese with a high fat content, and one ice cream containing chocolate of all samples showed the discrepancy. Our result suggest that TEMPO®TVC system is efficient to analyses total aerobic bacteria to compare manual method in time-consuming and laborious process except livestock products having limit of detection.

This study was designed to evaluate a repeated oral dose toxicity and immunomodulating activity of Pulsatilla koreana and Artemisiae annuae in Sprague-Dawley rats. The female rats were treated with Pulsatilla koreana and Artemisiae annuae of control group, low group (0.5 ml/kg), medium group (1 ml/kg), high group (2 ml/ kg) for distilled water, intragastrically for 4 weeks, respectively. To ensure the safety of Pulsatilla koreana and Artemisiae annuae such as the following were observed and tested. We examined the body weight, the feed intake, the clinical signs, the ophthalmological test, the hematological and the serum biochemical analysis. We also observed the histopathological changes of liver and kidney in rats. Hematological results were the increase of neutrophils, lymphocytes and monocytes in the high dose group of Pulsatilla koreana. The increase immune cells in the high dose group of Pulsatilla koreana might immunomodulating activity. No significant differences in body weight, feed intake, serum biochemical analysis and histopathological between control and fed group were found. In conclusion, Pulsatilla koreana and Artemisiae annuae is physiologically safe and improve immunomodulating activity.

This study was investigated to determine the contamination levels of total aerobic bacteria, coliform group, E. coli and food-borne pathogens of side dishes from 2 traditional markets (100 samples) and 2 super markets (100 samples) located on Ulsan. The levels (range) of total aerobic bacteria was 4.75 log CFU/g (1.60~6.92 log CFU/g) in traditional market and 4.62 log CFU/g (2.00~6.46 log CFU/g) in super market, respectively. Coliform was detected in 64 and 66 samples sold at traditional markets and super markets, respectively. E, coli was detected in 4 and 6 samples sold at traditional markets and super markets, respectively. The food-borne pathogens, namely Bacillus cereus and Listeria monocytogenes were detected in 1 sample sold at traditional markets, respectively, and Bacillus cereus was detected in 4 samples sold at super markets. However, other pathogens such as Salmonella spp., Shigella spp., Vibrio parahaemolyticus, Yersinia enterocolitica, Clostridium perfringens, Camphylobacter jejuni and Pathogenic E. coli were not detected. The Saengchae and Seasoned Jeotgal were relatively vulnerable compared to the others in the food-borne pathogens.

The present study was evaluated the antibacterial effect of the combination of Coptidis rhizoma, Glycyrrhiza uralensis Fischet, Schizandra chinensis and Corni Fructus(1:1:1) extracts(CGSC10). Furthermore, the effectiveness of CGSC10, sodium chlorate, and the combination of CGSC10 and sodium chlorate(CGSCS10) against E. coli O157:H7 infection was studied using ICR female mice. During the incubation period, the dose of 5, 10, and 20% CGSC10 was inhibited the growth of E. coli O157:H7 by 34.7, 60.2, and 76.4%, respectively. For 7 days after single challenge with E. coli O157:H7, forty female ICR mice were divided into four experimental groups which were administered in drinking water with saline, 10% CGSC10, 15 mM sodium chlorate, and CGSCS10, respectively. On the 3rd day, the number of E. coli O157:H7 in mouse feces was significantly decreased by administration of CGSC10, 15 mM sodium chlorate, and CGSCS10 (p < 0.001). On the 7th day-after administration, CGSC10, sodium chlorate, and CGSCS10 were decreased the number of E. coli O157:H7 by 27.1, 67.7, and 83.3%, respectively. According to the results of the present study, administration of CGSCS10 to mice can reduce the severity of E. coli O157:H7 infection. In addition, it is suggested that CGSCS10 represents a good candidate for the treatment of enteric infections in domestic animals.

Fish contain both the neurotoxin methyl mercury (MeHg) and nutrients important for brain development. The developing brain appears to be most sensitive to MeHg toxicity and mothers who consume fish during pregnancy expose their fetus prenatally. Although brain development is most dramatic during fetal life, it continues for years postnatally and additional exposure can occur when a mother breast feeds or the child consumes fish. This raises the possibility that MeHg might influence brain. We evaluated the relationship between fish consumption and mercury exposure levels in umbilical cord blood of the pregnant women of the city of Tongyeong city, Korea. A total of 159 pregnant women residing in the city of Tongyeong, Korea were recruited for the study between October 2010 and March 2011. Fish consumption was evaluated using food frequency questionnaires including detailed questions on fish consumption. We used ANOVA to estimated the particular relevance between the frequency of fish consumption and the umbilical cord blood mercury concentration, and other various factors. The average mean concentration of mercury levels in umbilical cord blood of pregnant women who participated in our study were 2.69 ± 2.50 ppb, ranging from 0.01 to 14.80 ppb. The mean concentration of umbilical cord blood mercury exposure was lower than the level recommended by WHO (5.0 ppb), but the mercury exposure level exceeded the WHO recommended in 17 (10.7%) cases of umbilical cord blood. Mercury levels in cord blood of pregnant women were 2.04 ± 2.00 ppb, ranging from 0 to 8.00 ppb in below 29 years old and 3.18 ± 2.74 ppb, ranging from 0.01 to 14.80 ppb in more 30 years old. In this study, there was a significant difference for the frequency of eating fish between the groups (p < 0.01). The level of the groups that ate fish 3 to more times per week (4.15 ± 4.02 ppb) was significant higher as compared with the level of other groups that ate fish 1 to times per week (2.63 ± 2.22 ppb) and none per week (1.06 ± 1.44 ppb), respectively. We found that the mercury concentration of umbilical cord blood associate with fish consumption and this was statistically significant and this fact revels that fish consumption is positively related to mercury levels in the umbilical cord blood. We need systematic and periodic research on the general population to prevent mercury poisoning, which can be cause by low-level mercury exposure from dietary intake such as chronic fish consumption

In this study, a method was developed using molecular biological technique to distinguish an authenticity of meats for processed meat products. The genes for distinction of species about meats targeted at 12S or 16S genes in mitochondrial DNA and the species-specific primers were designed by that PCR products' size was around 200bp for applying to processed products. The target materials were 10 species of livestock products and it checked whether expected PCR products were created or not by electrophoresis after PCR using species-specific primers. The results of PCR for beef, pork, goat meat, mutton, venison, and horse meat were 131, 138, 168, 144, 191, and 142 bp each. The expected PCR products were confirmed at 281, 186, 174, and 238 bp for chicken, duck, turkeymeat, and ostrich. Also, non-specific PCR products were not detected in similar species by species-specific primers. The method using primers developed in this study confirm to be applicable for composite seasoning including beefs and processed meat products including pork and chicken. Therefore, this method may apply to distinguish an authenticity of meats for various processed products.

Antibiotic resistance in animal isolates of enterococci is a public health concern, because of the risk of transmission of antibiotic-resistant strains or resistance genes to humans through the food chain. This study investigated phenotypic and genotypic resistances profile of tetracycline in 245 Enterococcus isolates from bovine milk. A total of 245 enterococci were isolated from 950 milk samples. The predominant strain was E. faecalis (n = 199, 81.2%) and E. faecium (n = 25, 10.2%). E. avium (n = 7, 2.9%), E. durans (n = 6, 2.5%), E. gallinarum (n = 4, 1.6%), and E. raffinosus (n = 4, 1.6%) were also isolated. Of the 245 enterococcal isolates 76.3% (n = 187) displayed tetracycline resistance (≥ 16 μg/ml). Of the 187 tetracycline-resistant isolates, 83.4% (n = 156), 16.1% (n = 30), and 26.7% (n = 50) possessed the genes tet(M), tet(L), tet(S) respectively. While 3.2% (n = 6) of the tetracycline- resistant isolates possessed all three genes tet(M) + tet(L) + tet(S), 8.6% (n = 16), 16.0% (n = 30), and 2.7% (n = 5) of them possessed two genes tet(M) + tet(L), tet(M) + tet(S), and tet(L) + tet(S) respectively. The tetracycline resistance pattern investigated in this study was attributable mainly to the presence of tet(M).

This study is a survey of regulatees' perception on main contents and enforcement effect of the Act, the retailers at food stores within Green Food Zone, for the effective enforcement of “The Special Act on the Safety Management of Children's Dietary Life” legislated in 2008. Percentage, frequency analysis, and T-test are derived from the survey carried out to the 175 retailers at food stores within Green Food Zone, across the country except Jejudo, for the two months March and April of 2011. The survey results are as follows. Over 80% of the respondents are aware of comprehensive policy for child food safety and the enforcement of 'the Special Act' and considering the rate of satisfaction on food safety information provided by government, over 90% of the respondents, virtually most of them, are satisfied. The rate of awareness of Green Food Zone is about 80% and that of outstanding business for children is about 50%, showing little perception of it. The comparison of the survey result of 2011 with that of 2010, which have the same questions to the retailers within Green Food Zone, still indicates a need for enhancement of understanding on absolute standard, though it showed fairly better improvement in general. Several proposals are given in this study based on the survey results, which will contribute to the children food safety and health improvement in the end.

Salmonella spp. and Brucella spp. have caused a considerable disease of farmed animals and economic loss in animal farming and food industry. In this study, the disinfection efficacy of Vital-Oxidel®, a commercial disinfectant, composed to chlorine dioxide, betaine hydrochloride, and propylene glycol was evaluated against S. typhimurium and Brucella ovis. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at 4oC. Vital-Oixdel® and test bacteria were diluted with distilled water (DW), hard water (HW) or organic matter suspension (OM) according to treatment condition. On OM condition, the bactericidal activity of Vital-Oixdel® against S. typhimurium and Brucella ovis was lowered compared to that on HW condition. As Vital-Oxidel® possesses bactericidal efficacy against animal pathogenic bacteria such as S. typhimurium and Brucella ovis, this disinfectant solution can be used to control the spread of bacterial diseases.

This study was carried out to investigate the toxicity of food Red No.2 in the Sprague-Dawley (SD) female rat for 4 weeks. SD rats were orally administered for 28 days, with dosage of 500, 1,000, 2,000 mg/kg/day. Animals treated with food Red No.2 did not cause any death and show any clinical signs. They did not show any significant changes of body weight, feed uptake and water consumption. There were not significantly different from the control group in urinalysis, hematological, serum biochemical value and histopathological examination. In conclusion, 4 weeks of the repetitive oral medication of food Red No.2 has resulted no alteration of toxicity according to the test materials in the group of female rats with injection of 2,000 mg/kg. Therefore, food Red No.2 was not indicated to have any toxic effect in the SD rats, when it was orally administered below the dosage 2,000 mg/kg/day for 4 weeks.

The current standard for testing tetrodotoxin (TTX) in foodstuffs is the mouse bioassay (MBA) in Korea as in many other countries. However, this test suffers from potential ethical concerns over the use of live animals. In addition, the mouse bioassay does not test for a specific toxin thus a sample resulting in mouse incapacitation would need further confirmatory testing to determine the exact source toxin (e.g., TTX, STX, brevotoxin, etc.). Furthermore, though the time of death is proportional to toxicity in this assay, the dynamic range for this proportional relationship is small thus many samples must be diluted and new mice be injected to yield a result that falls within the quantitative dynamic range. Therefore, in recent years, there have been many efforts in this field to develop alternative assays. High performance liquid chromatography (HPLC) coupled with mass spectrometry (MS) has been emerged as one of the most promising options. A LC-MS-MS method involves solid-phase extraction (SPE) and followed by analysis using an electrospray in the positive ionization mode and multiple reactions monitoring (MRM). To adopt LC-MS-MS method as alternative standard for testing TTX, we performed a validation study for the quantification of TTX in puffer fish. This LC-MS-MS method showed good sensitivity as limits of detection (LOD) of 0.03~0.08 μg/g and limits of quantification (LOQ) of 0.10~0.25 μg/g. The linearity (r2) of tetrodotoxin were 0.9986~0.9997, the recovery were 80.9~103.0% and the relative standard deviations (RSD) were 4.3~13.0%. The correlation coefficient between the mouse bioassay and LC/MS/MS method was higher than 0.95.

This study was conducted to evaluate the microbiological hazard on nursery school children's hands and to investigate the reduction effect of hand washing education. A total of 59 nursery school children's hands were tested. The average number of total aerobic bacteria was 3.72 ± 0.38 log CFU/hand. Five children's hands(2 male and 3 female) were positive(14.3%) for the coliform bacteria. These results showed that hand washing education are required repetitively. Among the pathogenic bacteria tested in this study, Staphylococcus aureus and Bacillus cereus were detected in 9(25.7%) and 16(45.7%) out of 35 their hands, respectively. Twelve out of sixteen B. cereus isolates(70.0%) produced enterotoxin. The results indicate that the hand hygiene of nursery school children needs to be improved. Comparing before and after hand washing in educated and non-educated group, the reduction effect of total aerobic bacteria on their hands was 0.42 and 0.60 log CFU/hand, respectively. The educated group showed 0.18 log CFU/hand higher reduction effect than non-educated group but microorganism did not eliminate perfectly. From the results, using a hand sanitizer after washing with soap and the continuous hand washing education are required to control the contaminated bacteria on nursery school children's hands.

A total of 187 samples of leafy vegetables and fruits were acquired at traditional markets and department stores in Seoul, Korea. Samples were tested for microorganism distributions and for the presence of pathogenic bacteria. The aerobic mesophilic counts ranged between 2.5 and 9.4 log CFU/g, with the highest count recorded from the dropwort. Counts of psychrotrophic microorganisms were as high as those of the mesophilic microorganisms. Total coliform populations between 1.0 and 7.8 log CFU/g were found in 90.9% of the samples. Microbiological counts for fruits were very low. Escherichia coli was isolated in 24 (12.8%) samples. Staphylococcus aureus and Clostridium perfringens contamination were found in 15 (8.0%) and 20 (10.7%) samples. Salmonella species and Listeria monocytogenes were detected in 2.7 and 0.5% of samples, respectively. Among the total 187 samples, 8 samples were contaminated by more than two pathogens. E. coli O157:H7 was not detected in any of the samples. The microbial contamination levels determined in the present study may be used as the primary data to execute microbial risk assessment of fresh vegetables and fruits.

Highly pathogenic avian influenza virus (HPAIV) is already panzootic in poultry and caused a considerable economic loss in poultry industry. In addition, HPAIV continues to cross species barriers to infect humans and other mammals, often with fatal outcomes. In this study, the virucidal efficacy of Citra-Kill® composed to quaternary ammonium chloride and citric acid was investigated against avian influenza H9N2 virus (AIV). A virucidal efficacy was determined with the viability of AIV contacted with the disinfectant in the allantoic membrane of chicken embryos. Citra-Kill® and AIV was reacted on the distilled water (DW), hard water (HW) or organic matter suspension (OM) condition. On DW condition, AIV was inactivated with 2,000 fold dilutions of Citra-Kill®. When the antiviral effect on HW condition was evaluated, the antiviral activity of the disinfectant showed on 1,500 fold dilutions against AIV. With the investigation of the antiviral effect of the disinfectant on OM condition, AIV was inactivated on 500 fold dilutions of Citra-Kill®. As Citra-Kill® possesses virucidal efficacy against AIV, the disinfectant solution can be used to limit the spread of animal viral diseases.