The objective of this study is to evaluate the antioxidant components, elastase inhibition activities, and melanin synthesis rates of Korean traditional Actinidia (Actinidia arguta) fruits and leaves depending on the ethanol extraction concentrations. The total polyphenol content was the highest in the 50% ethanol extract of both fruits and leaves, with values of 634.1 mg GAE/100 g and 3,985.2 mg GAE/100 g, respectively. The total flavonoid content was the highest in the fruit 90% extract and leaf 50% extract at 191.9 mg/100 g and 2655.6 mg/100 g, respectively. The vitamin C content was the highest in the 50% extract of leaves at 2990.3 mg/100 g. Elastase inhibition was the highest at 56.9% in the leaf 50% extract at a concentration of 1,000 μg/mL. Melanin synthesis inhibition showed the highest melanin synthesis inhibitory effect among the extracts, as the leaf 50% extract showed an inhibitory rate of 65% or more. Therefore, the antioxidant components, elastase inhibition activities, and melanin synthesis inhibitory rate were better in leaves than in fruits. The leaf 50% extract was particularly the best among the extracts. Korean traditional Actinidia leaves can be considered as potential sources for new functional materials.

본 연구는 후박나무 용기묘를 대상으로 전광, 35%, 55%, 75% 차광수준에 따른 생장 특성 및 생리적 반응을 알아보고자 실시하였다. 후박나무 묘목은 차광 시 상대적으로 지상부의 생장특성을 보여주는 지표들이 높은 경향을 보였으며, 전광에서는 뿌리와 관련된 지표인 뿌리건중비, 뿌리생장에 서 유의적으로 높은 경향을 보였다. 또한, 전광에서의 낮은 광보상점과 높은 순양자수율은 이미 후박나무가 내음성을 가지고 있고, 광이 부족한 환경에 노출된 차광처리구들에서 암호흡량의 감소, PIABS 및 SFIABS의 증가로 광합성 기구의 활력을 효율적으로 증가시키는 것을 알 수 있다. 한편, 전광은 지상부 비율의 증가, 낮은 T/R율과 가장 높은 순광합성속도를 보여 광합성으로 합성되는 탄수화물이 지상부 뿐만 아니라 지하부의 생장 역시 증가시킨 것을 알 수 있는 결과였다. 따라서 후박나무 용기묘는 전광조건에서 생장 및 생리적 특성이 가장 양호한 경향을 보였다.

Cyanobacterial resting cells, such as akinetes, are important seed cells for cyanobacteria’s early development and bloom. Due to their importance, various methods have been attempted to isolate resting cells present in the sediment. Ludox is a solution mainly used for cell separation in marine sediments, but finding an accurate method for use in freshwater is difficult. This study compared the two most commonly used Ludox methods (direct sediment treatment and sediment distilled water suspension treatment). Furthermore, we proposed a highly efficient method for isolating cyanobacterial resting cells and eDNA amplification from freshwater sediments. Most of the resting cells found in the sediment were akinete to the Nostocale and were similar to those of Dolichospermum, Cylindrospermum, and Aphanizomenon. Twenty times more akinetes were found in the conical tube column using the sediment that had no treatment than in the sample treated by suspending the sediment in distilled water. Akinete separated through Ludox were mainly spread over the upper and lower layers in the column rather than concentrated at a specific depth in the column layer. The mibC, Geo, and 16S rDNA genes were successfully amplified using the sediment directly in the sample. However, the amplification products of all genes were not found in the sample in which the sediment was suspended in distilled water. Therefore, 5 g to 10 g of sediment is used without pretreatment when isolating cyanobacterial resting cells from freshwater sediment. Cell isolation and gene amplification efficiency are high when four times the volume of Ludox is added. The Ludox treatment method presented in this study isolates cyanobacterial resting cells in freshwater sediment, and the same efficiency may not appear in other biotas. Therefore, to apply Ludox to the separation of other biotas, it is necessary to conduct a pre-experiment to determine the sediment pretreatment method and the water layer where the target organism exists.

This study compares the abundance and community structure of zooplankton organisms from pelagic regions, and considers particularly the trophic levels vs. zooplankton abundances and biomass. Zooplankton samples were collected three times from May to November 2022, at 30 temperate lakes and reservoirs, which belong to four different river basins. The total zooplankton abundance, biomass and species index were showed considerable spatial variation. The spatial pattern of rotifer abundance was similar to that of total zooplankton abundance, while there were not showed similar patterns of zooplankton biomass (μg L-1) in lentic ecosystems. The rotifer strongly dominated the zooplankton assemblage in smaller lentic system than that of larger. A total of 130 species of zooplankton were identified (83 rotifers, 34 cladocerans and 13 copepods). The total average of zooplankton abundance and biomass were 213.7±342.3 Ind. L-1 (n=129) and 1382.8±1850.4 μg L-1, respectively. Total and average of zooplankton abundance were usually dominated by the rotifers (>56.9%), while those of zooplankton biomass were dominated by the cladocerans and copepods (>73.6%) in lentic ecosystems. Considering the Trophic State Index (TSI), the factors of zooplankton abundance and biomass were included in between meso- and eutrophic states (27 lakes, 90% of all). The mean abundance and biomass of zooplankton in eutrophic systems were higher than that of meso- and hypertrophic systems. From this result, we suggest that management strategy for the lentic ecosystem water environment has to be focused more on small-sized lakes and reservoirs, in terms of zooplankton assemblages.

Recently, Korean government has introduced Multi Metric Indices (MMI) using various biocommunity information for aquatic ecosystem monitoring and ecosystem health assessment at the national level. MMI is a key tool in national ecosystem health assessment programs. The MMI consists of indices that respond to different target environmental factors, including environmental disturbance (e.g. nutrients, hydrological and hydraulic situation of site etc.). We used zooplankton community information collected from Korean lakes to estimate the availability of candidate zooplankton MMI indices that can be used to assess lake ecosystem health. First, we modified the candidate indices proposed by the U.S. EPA to suit Korean conditions. The modified indices were subjected to individual index suitability analysis, correlation analysis with environmental variables, and redundancy analysis among indices, and 19 indices were finally selected. Taxonomic diversity was suggested to be an important indicator for all three taxonomic groups (cladoceran, copepod, rotifer), on the other hand, the indices using biomass for large cladocerans and copepods, while the indices using abundance were suggested for small cladocerans and rotifers.

Environmental DNA (eDNA) can exist in both intracellular and extracellular forms in natural ecosystems. When targeting harmful cyanobacteria, extracellular eDNA indicates the presence of traces of cyanobacteria, while intracellular eDNA indicates the potential for cyanobacteria to occur. However, identifying the “actual” potential for harmful cyanobacteria to occur is difficult using the existing sediment eDNA analysis method, which uses silica beads and cannot distinguish between these two forms of eDNA. This study analyzes the applicability of a density gradient centrifugation method (Ludox method) that can selectively analyze intracellular eDNA in sediment to overcome the limitations of conventional sediment eDNA analysis. PCR was used to amplify the extracted eDNA based on the two different methods, and the relative amount of gene amplification was compared using electrophoresis and Image J application. While the conventional bead beating method uses sediment as it is to extract eDNA, it is unknown whether the mic gene amplified from eDNA exists in the cyanobacterial cell or only outside of the cell. However, since the Ludox method concentrates the intracellular eDNA of the sediment through filtration and density gradient, only the mic gene present in the cyanobacteria cells could be amplified. Furthermore, the bead beating method can analyze up to 1 g of sediment at a time, whereas the Ludox method can analyze 5 g to 30 g at a time. This gram of sediments makes it possible to search for even a small amount of mic gene that cannot be searched by conventional bead beating method. In this study, the Ludox method secured sufficient intracellular gene concentration and clearly distinguished intracellular and extracellular eDNA, enabling more accurate and detailed potential analysis. By using the Ludox method for environmental RNA expression and next-generation sequencing (NGS) of harmful cyanobacteria in the sediment, it will be possible to analyze the potential more realistically.

This experiment was conducted to confirm the possibility of preparing Sorghum×sudangrass hybrid artificial hay using far-infrared rays in Korea. The machine used in this experiment is a drying device based on far-infrared rays, and is designed to control temperature, air flow rate, far-infrared radiation amount, and air flow speed. The Sorghum×sudangrass hybrids harvested in late September were wilted in the field for one day, and a drying test was performed on them. Conditions for drying were performed by selecting a total of 7 conditions, and each condition induced a change in radiation amount in a single condition (42%) and two steps (4 treatments) and three steps (2 treatments). The speed of the air flow in the device was fixed at 60 m/s, and the run time was changed to 30, 60, and 90 minutes. The average dry matter (DM) content was 82.84%. The DM content was 59.94 and 76.91%, respectively, in drying conditions 1 and 3, which were not suitable for hay. In terms of drying rate, it was significantly higher than 80% in the 5, 6 and 7 treatment, and power consumption was slightly high with an average of 5.7 kw/h. As for the feed value according to each drying condition, the crude protein (CP) content increased as the drying time increased, and there was no significant difference between treatments in ADF, NDF, IVDMD and TDN content. In terms of RFV, treatment 1, which is a single condition, was significantly lower than the complex condition. Through the above results, it was determined that the drying conditions 4 and 5 were the most advantageous when considering the drying speed, power consumption, and quality.

Broad bean wilt virus 2 (BBWV2) is a species in the genus Fabavirus and family Secoviridae, which is transmitted by aphids and has a wide host range. The BBWV2 genome is composed of two single-stranded, positive-sense RNAs, RNA-1 and RNA-2. The representative symptoms of BBWV2 are mosaic, mottle, vein clearing, wilt, and stunting on leaves, and these symptoms cause economic damage to various crops. In 2019, Perilla fructescens leaves with mosaic and yellowing symptoms were found in Geumsan, South Korea. Reverse-transcription polymerase chain reaction (RTPCR) was performed with specific primers for 10 reported viruses, including BBWV2, to identify the causal virus, and the results were positive for BBWV2. To characterize a BBWV2 isolate (BBWV2-GS-PF) from symptomatic P. fructescens, genetic analysis and pathogenicity tests were performed. The complete genomic sequences of RNA-1 and RNA-2 of BBWV2-GS-PF were phylogenetically distant to the previously reported BBWV2 isolates, with relatively low nucleotide sequence similarities of 76-80%. In the pathogenicity test, unlike most BBWV2 isolates with mild mosaic or mosaic symptoms in peppers, the BBWV2-GS-PF isolate showed typical ring spot symptoms. Considering these results, the BBWV2-GS-PF isolate from P. fructescens could be classified as a new strain of BBWV2.

본 연구에서는 석문간척지 주변 농가들을 대상으로 간척지 논 범용화를 위한 암거공법 관련 세부 기술요인에 대한 농가 수요조사 및 분석을 통해 다음과 같은 결과들을 도출하였다. 첫째, 지하흡수공을 보다 필요하고 중요하게 인식할수록 해당 기술에 대한 수용의사가 증가하는 것으로 나타났다. 둘째, 지하배수를 보다 필요하고 중요하게 인식할수록 해당 기술에 대한 수용의사가 증가하는 것으로 나타났다. 셋째, 지하관수를 보다 필요하고 중요하게 인식할수록 해당 기술에 대한 수용의사가 증가하는 것으로 나타났다. 넷째, 심토파쇄를 보다 중요하게 인식할수록 해당 기술에 대한 수용의사가 증가하는 것으로 나타났다. 다섯째, 지하흡수공 기술에 대한 수용의사가 높은 농가들이 무굴착암거공법 수용확률이 높은 것으로 나타났다.

본 연구의 주요 목적은 회귀기반의 다양한 머신러닝 알고리즘을 개발하고 다양한 농업 분야에서 사용되는 트랙터의 연료 소비량을 예측하는 것이다. 비포장 도로주행 농업 기계중에서도 사용 비중이 가장 높은 트랙터를 선정하였다. 실제 농가에 방문하여 현업 전문가 조언을 바탕으로 연구하여 설문지를 작성하였으며, 설문 대상은 경남 사천시에 있는 농가 10곳, 진주시에 있는 농가 62곳 등, 총 72곳의 농가이다. 농작업으로는 벼농사, 보리농사, 밭농사 등이 있으며, 작업내용으로는 쟁기, 로터리, 비료살포, 베토, 모내기작업 등이 있다. 다중 회귀분석을 통해 연료 소비량 예측에 영향을 미치는 변수(마력, 기계사용연수, 경작면적, 작업 시간)를 추출하였고. 머신러닝 회귀 학습기 모형으로 학습하여 예측 모형의 성능을 검증하였다. 연료 소비량을 예측하는 모델의 성능은 결정 계수(R), RMSE (제곱 평균 제곱근 오차), MSE (평균 제곱 오차) 및 MAE (평균 절대 오차)를 포함한 4가지 통계적 품질 매개변수를 사용하여 결정되었다. 연구 결과 4가지 모델(다중회귀, 랜덤포레스트, 아다부스트, K-최근접 이웃) 중 K-최근접 이웃의 성능이 제일 높은 것으로 나타났다. 결론적으로 본 연구의 결과는 실제 농가의 연료 소비량을 예측하여 면세유 유통의 투명성을 확보하고 추후 개발 모델의 의사결정에 활용될 수 있을 것으로 기대된다.

Sustainable and healthy diet is a challenge in recent world. Despite the global depletion of water resources, Korea has no system for controlling its water footprint. This study established the water footprint tables of Korean food using the Water Footprint Network databases, and applied them into two meal plans for 19~64 year-old adults recommended in the Dietary Reference Intakes for Koreans 2020. Nut, oil, and meat’s water footprints were higher and those of fruit and vegetable were lower. Sesame oil had the highest water footprint of 21,793 L/kg and pineapple had the lowest domestic water footprint of 102 L/kg. Water footprint of one serving size of beef was 925 L, that of chicken was 260 L, and those of soybean were 43 L in global and 81 L in domestic. The water footprint of the recommended 2,400 kcal meal plan was 2,882 L, and that of 1,900 kcal meal plan was 1,915 L. The water resources can be saved by choosing food with lower water footprint. The results of this study can be used in the further researches for more sustainable and healthier Korean diet.

The purpose of this study was to analyze the physicochemical properties by mixing ratio of soybean and peanut protein to secure basic data for developing alternative protein foods. As a result of analyzing the protein molecular weight pattern, it was confirmed that the specific molecular weight was affected by the soybean and peanut protein mixture. The content of glutamic acid, aspartic acid, arginine, glycine, serine, alanine, and tyrosine among nonessential amino acids was higher as the mixing ratio of peanut protein to soy protein was higher. However, the higher the peanut protein mixing ratio, the lower the water absorption capacity. Based on the results of this study, further studies, such as selecting soybean and peanut cultivars for determining the optimal mixing ratio of soybean and peanut protein and processing methods to improve physical properties, are necessary

The purpose of this research is to investigate Korean architects’ perception of the Modern Movement in architecture in the West during the Japanese colonial period, by analyzing two Korean publications in the 1930s: Park Dongjin’s ‘Present Architectural Tendency’ (Dong-A Ilbo, March 1931) and Hong Yunsick’s ‘Trend in the Thought of Moderne Baukunst’ (Chogwang, September 1937). As a result of the investigation, it is confirmed that the two men welcomely accepted the universal modernity, regarding the rational and functional - rather than subjective expressions of the individual - as the key to modern architecture. Although their perception of the Modern Movement in Western architecture was inevitably superficial due to the limited condition of the Japanese colonial period in Korea, there was an obvious advancement in the latter’s perception from the former’s, reflecting the progress in knowledge of it over the six and a half years between the two. Therefore, it is argued that their 1930s’ writings are meaningful as the first Korean publications that illustrate how Korean architectural circles perceived the contemporary architectural movement in the West.

To investigate the anti-inflammatory activity of submerged culture using Cordyceps militaris mycelium, culture-including mycelia was extracted and lyophilized into postbiotics (hot-water extract; CM-HW). HW was fractionated into crude polysaccharide (CM-CP) by ethanol precipitation, and CM-CP was further dialyzed into CM-DCP by dialysis with running water using 12~14 kDa dialysis tube. When the cytotoxicity of subfractions against cells was assessed, no subfraction had a cytotoxic impact that was substantially different from the control groups. In an inflammatory model using LPS-stimulated RAW 264.7 cells, CM-DCP significantly decreased IL-6 and MCP-1 production levels compared to the LPS-control group. CM-DCP also inhibited IL-6 and IL-8 secretion in HaCaT keratinocytes stimulated with TNF-α and IFN-γ. In the meanwhile, the neutral sugar content and mannose ratio of anti-inflammatory CM-DCP were higher than the other fractions, and CM-DCP contained β-1,3/1,6-glucan of 216.1 mg/g. High pressure size exclusion chromatography revealed that CM-DCP contained molecules with a molecular weight range of 5.6 to 144.0 kDa. In conclusion, postbiotics of C. militaris mycelium significantly promoted anti-inflammatory activity, suggesting that neutral polysaccharides including Glc and Man contribute to the anti-inflammation in RAW 264.7 or HaCaT cells.

본 연구에서는 여러가지 경영, 재무활동들 중 해외 교차상장(cross-listing)이 국내기업의 비효율적 자본지출을 개선할 수 있는지 검증하고, 그 영향을 분석하였다. 특히, 해외 교차상장과 관련한 내생성 문제를 해소하기 위해 교차상장을 한 기업(교차상장기업)과 그렇지 않은 기업(비교차상장 기업)을 선정하고 이중차분법(Difference-in- Differences Analysis)을 활용하여 두 그룹의 비효율적 자본지출과 기타 주요 재무지표들을 분석하였다. 주요 실증분석 결과에 따르면 교차상장기업은 교차상장이 된 후 비교차상장기업에 비해 총자본지출과 비효율적 자본지출이 감소하는 것으로 나타났다. 또한, 교차상장기업의 성장성과 효율적 투자지표인 Tobin’s Q와 R&D 투자지출 모두 비교차상장기업 대비 증가하여 교차상장의 긍정적인 효과를 확인할 수 있었다. 반면, 교차상장기 업의 수익성은 교차상장 이후 비교차상장기업 대비 하락하였는데 이는 교차상장을 위한 실적 부풀리기 등의 영향 으로 해석하였다. 본 연구를 통해 해외 교차상장이 한국기업의 비효율적 자본지출과 여러 재무활동에 유의한 영향을 끼치는 것을 확인할 수 있었다. 즉, 기업의 교차상장은 시장과 투자자들에게 더 많은 정보를 제공하여 대리인문제, 정보불균형 등으로 인한 문제들을 해소시키는 효과가 있으며, 보다 엄격한 해외상장의 요건을 충족시키기 위해 기업지배구조 또한 개선되는 효과가 존재한다고 할 수 있다.

수도권 중심의 국가발전 전략이 한계에 다다른 가운데, 주요국들은 지 방을 발전시키기 위한 다양한 전략이 제시되어 왔다. 2000년대 이후 유 럽, 일본 등 주요국들은 광역경제권 형성을 통한 지방 대도시권 발전방 향의 전략을 채택해 온 가운데, 최근 한국에서도 부울경 지역연합이 출 범한 경험이 있다. 본 논문에서는 프랑스의 지방분권 개혁 및 문제점, 과 제에 관하여 고찰하였다. 특히 본고에서는 프랑스의 광역경제권 형성 과 정을 통해 부·울·경 메가시티 전략에 대한 시사점을 얻고자 하였다. 1970년대 중앙집권적 행정체제로는 경제발전과 지역협력 대응에의 한계 가 있음을 직시한 프랑스는 지방분권을 추진해 왔다. 지방분권 추진에 많은 어려움을 겪어 왔던 프랑스 정부는 2012년 이후 최근까지 지방분 권의 효율성과 민주주의의 조화를 위한 분권발전 정책을 제시해 왔다. 프랑스는 지방분권 추진 과정에서 지역 광역화와 생활권의 이격에서 발 생하는 문제점들을 해결하기 위해, 도시지역계획(PLU), 코뮌 토지이용구 분도(CC), 국토의 일관성 계획(SCOT) 등을 제시하였다.

In this study, a finite element analysis was used to analyze the stress state and vibration characteristics generated by continuous contact between wheels and rails when driving urban railway vehicles. The rails applied to the analysis were divided into straight and curved shapes, and three-dimensional modeling was performed to analyze the changes in structural characteristics of wheels and rails when driving on straight and curved rails. As a result of the analysis, the stress characteristics were up to 6.5 MPa on a straight rail and 9.81 MPa on a curved rail, and it is believed that this increase in stress will increase noise due to an increase in friction at the interface. The vibration characteristics of the wheels and rails showed similar behavior from the 3rd mode to the 9th mode of the rail to the intrinsic vibration characteristics from the 4th mode to the 6th mode of the wheel.