The purpose of this study was to estimate beverage consumption frequency and determine related problems of elementary school students in Chungnam. The survey was conducted with 488 students(boys=230, girls=258) using a questionnaire. The average age of the students was 10.1 years old, thir average height and weight were 145.1 ㎝ and 39.5 ㎏, respectively and the obesity index was -1.3%. The frequencies for breakfast, lunch and dinner were 5.8 times/week, 6.7 times/week and 6.4 times/week, respectively. Regarding the frequency of snacks, high response rates for ``once/day``(38.3%) and ``none`` (30.5%) were observed. Nutrition label on beverages were identified rarely(39.1%), nearly never(19.7%), and never(12.9%). The choice factors in selecting beverage were taste, nutrition, and price. For the type of snack most often consumed, fruit or juice was 25.8%, snack items were 25.2%, bread was 24.8%, dairy products were 7.6%, beverages were 7.0%, noddles were 6.2%, and rice cakes were 3.5%. The kinds of favorite beverages were carbonated drinks(30.3%), functional drinks (24.4%) and milk and yoghurt(23.8%), and main reason for the choice of beverage was "it tastes good". For the point of time that students wanted to drink a beverage, "when I feel thirsty" appeared most often for dairy products and beverages and the next most frequent answer was "after exercise". Therefore it may be necessary to administer systematic nutritional education on perception on nutrition fact label of beverage. And it is thought that plan for proper beverage consumption in elementary school students may be established.

This study was conducted to establish process conditions for plant-originated lactic acid production using a mixed culture of plant originated lactic acid bacteria, Lactobacillus sakei B2-16, and Lactobacillus plantarum P23, which were isolated from kimchi, and Bacillus subtilis, which was TP6 isolated from Denjang. Soybean medium was pretreated for 10 minutes at 110℃ and hydrolyzed with 0.2%(w/v) cellulase at 55~60℃ for at least 2 hrs. The quality of the final fermentation product was influenced by the inoculation ratio of the Lactobacillus sakei B2-16, Lactobacillus plantarum P23, and Bacillus subtilis TP6. The optimum microorganism inoculation ratio was 1:0.7:0.3, Lactobacillus sakei B2-16: Lactobacillus plantarum P23: Bacillus subtilis TP6, respectively. The sensory characteristics of the product were a refreshing sourness and a soft flavor.

This study was conducted to investigate the relationship between long naming of beverages and its effect on people's perception by gender. The survey was conducted in the Seoul area from March 10 to May 10, 2010. Approximately 59% of male and 41.8% of the female respondents were randomly selected from university students aged 20 to 29-years. Most (79.8%) of the students responding to the survey showed a preference for beverages. "Long-named beverages with ingredients listed" were considered the most reliable and ranked highest at 3.74, A significant difference was observed between males and females. "Long-named beverage that were made from domestic agricultural products" were regarded as the most healthful and ranked highest at 4.01. A significant difference between males and females was also observed. Long-naming influences a customer's purchasing tendency. In particular, women were more influenced by a sense of wellbeing when they purchase, because they are more interested in losing weight and being healthy.

본 연구에서는 홍삼음료의 다양화 및 기능성 강화와 여러 기능성을 가진 황기의 식품으로의 활용에 역점을 두고, 기존의 증점제를 대신하여 동결건조 천년초열매를 첨가한 새로운 기능성 홍삼음료을 개발하고자 하였다. 먼저 높은 항산화활성을 지닌 황기추출물을 얻고자 추출용매의 지용성정도를 달리하면서(100(증류수) : 0(95% 에탄올), 75 : 25, 50 : 50, 25 : 75) 80oC에서 추출한 추출물의 항산화활성을 측정한 결과, ABTS 및 DPPH radical 소거능 실험에서 증류수의 비율이 증가할수록 추출물의 항산화력이 증가하는 것으로 나타났다. 추출용매의 온도에 의한 황기추출물의 항산화활성 차이를 알아보고자 추출용액의 온도를 달리하면서(60, 80oC) 추출한 추출물의 항산화활성을 비교해 본 결과, 80oC에서의 추출물이 증류수와 에탄올혼합비율과는 상관없이 대부분 60oC 추출물보다 높은 값을 나타내었다. 기존의 점증제를 대신하여 다양한 기능성을 함유한 천연물 소재인 천년초분말을 선택하였으며, 구아검용액과 농도별 점도를 비교해 본 결과, 구아검 0.1%(w/v) 용액과 비슷한 점도를 나타내기 위해서는 천년초분말의 경우 1-2%(w/v)의 농도가 필요한 것으로 분석되었다. 황기와 천년초 첨가가 홍삼용액의 저장성(35oC)에 미치는 영향을 조사하기 위하여 이들 용액의 pH와 총미생물수를 7일 동안 측정하였다. 홍삼용액에 황기와 천년초를 첨가한 경우, 저장 1일째부터 pH가 급격히 감소하기 시작하였으며, 저장 3일째에는 pH가 약 3.6까지 떨어진 다음, 저장 6일 이후에는 다소 pH가 증가하였으며, 천년초분말을 첨가한 용액에서는 황기의 농도증가에 의한 유의적 pH 감소는 나타나지 않았다. 천년초를 첨가한 홍삼용액의 경우 황기의 농도와는 상관없이 저장 7일째 미생물의 성장을 관찰할 수 없었으며, 천년초를 첨가하지 않은 시료에 비해 6 log cycle 이상의 큰 살균효과를 나타내었다. 황기(3, 5%, w/v), 천년초(1.2%, w/v)를 함유한 홍삼용액(5%, w/v)이 대장암 및 뇌종양세포 증식에 미치는 영향을 각각 0.5 mg/mL과 1 mg/mL의 농도에서 살펴본 결과, 종양세포에 대한 의미 있는 증식억제효과는 나타나지 않았다.

The purpose of this study was to investigate the effects of perceived price and servicescape on employee service quality, food and beverage quality, and customer value in Daegu Korean restaurants and to suggest methods for them to become the food mecca of Korea and the world. The samples for this study were customers of nine Korean restaurants in Daegu. A total of 368 questionnaires were analyzed with factor analysis, a reliability test, and a covariance structural analysis. The results showed that perceived price was positively related to employee service quality and food and beverage quality and that servicescape was positively related to employee service quality and food and beverage quality. However perceived price, servicescape, employee service quality, and food and beverage quality were not related to customer value. The reasons were that these factors did not influence perceived customer value in Daegu Korean restaurants. The Daegu municipal office should require these restaurants to develop distinguished employee service and food and beverage quality and conduct consistent marketing to Koreans and westerners, so that these restaurants can become the food mecca of Korea and the world.

생강이 가지고 있는 항산화력을 최대화 시키기 위해 다양한 제조 공정에서 흑생강을 제조하였다. 증숙 온도 및 시간에 따른 DPPH radical 소거능을 측정하였고, 이를 반응표면 분석법에 의해 최적화된 제조 공정을 선정하였다. DPPH radical 소거능을 최대화 할 수 있는 최적 점을 설정한 결과 93.2oC에서 6시간의 증숙 공정이 설정되었다. 제조한 흑생강을 음료로 개발하기 위해, 매실 농축액과 꿀을 혼합하여 기능성과 선호도가 높은 음료를 제조하였다. DPPH radical 소거능, 플라보노이드 함량, 관능평가의 canonical 계수를 이용하여 수적 최적화를 통하여 최적 배합비를 구한 결과, 흑생강 추출물 14.2%, 매실농축액 5%, 꿀 10.8%로 나타났고 desirability가 0.615로 설정되었다. 이때의 종속 변수값들은 DPPH radical 소거능 46.0 mg/L, 플라보노이드 함량 29.9 mg/L, 선호도 5.284로 예측되었다.

생식 첨가량을 3.7-11.7%로 조절한 생식음료의 분산안정성을 터비스캔을 사용하여 광학적 방법으로 측정하였다. 생식음료는 제조 후 시간이 경과함에 따라 음료 하부에서는 침전이 생성되어 후방산란광플럭스(이후 후광플럭스로 약칭)가 강해진 반면, 중간부에서는 청징현상에 의해 약해졌으며, 최상층부에서는 거품이 생성되어 다시 강해졌다. 생식 첨가량이 증가할수록 침전이 서서히 진행되었으며 전체 침전층은 두텁게 형성되었다. 침전에 의한 후광플럭스의 퍼센트변화율의 시간에 따른 변화는 로그 모델이 적용되었으며 결정계수는 0.979-0.988로 높은 값을 보였다. 생식 첨가량은 생식음료의 상층부에 형성되는 청징층과 하층부의 침전층의 경계면, 즉 청징층 선단의 이동속도 뿐 만 아니라 분리된 층의 수에도 영향을 미쳤다. 생식 첨가량 3.7%의 경우는 2층으로, 5.7-9.7%의 경우는 3개 층으로, 11.7%는 4개 층으로 분리되었다. 생식음료 제조 후 17-29분 사이에 1차 청징층 발생이 시작되었고 22-53분간 지속되었으며, 생식첨가량이 낮을수록 청징층 두께는 증가하였다. 청징층 발생은 생식 첨가량 7.7%인 음료에서 가장 늦게 나타나 최대 분산안정성을 보였다.

다양한 식품군을 동결건조 또는 저온에서 건조하여 분말화한 생식은 물과 혼합 시 분산성이 저조하고 섭취방법이 불편하여 생식 소비 및 시장 확대에 제약이 있다. 생식의 섭취를 편의화하기 위하여 음료 타입의 생식을 개발하고자 생식 3종(BS, ES, SS)을 음료로 제조하여 품질을 비교하였다. 생식음료의 이화학적 특성과 관능적 특성을 조사한 결과 BS의 기호도가 가장 높았으며, 생식첨가량은 7.7%에서 기호도가 가장 우수하였다. 생식 BS를 사용한 음료의 경우 생식 함량이 증가함에 따라 점조도는 증가하였으며 함량 5.7% 이하의 음료는 팽창유체 양상을 보이다가 7.7%와 9.7%에서는 뉴튼유체와 유사한 특성을 나타내었고 11.7%에서는 뚜렷한 의가소성을 보였다. 생식음료 점조도의 시간의존성은 생식함량 5.7-9.7% 사이에서는 레오펙틱형 시간의존성이 관찰되었으나 3.7%와 11.7%에서는 시간 독립적이었다.

We investigated eight active natural antimicrobials for preservation of functional beverages that are usually degraded by yeasts rather than by bacteria due to a high sugar content and a low pH. Five strains of yeasts (S. cerevisiae, Z. bailii, P. membranaefaciens, C. albicans, and P. Anomala) were tested with eight natural antimicrobial agents (ε-polylysine, yucca extract, vitamin B1 derivative, scutellaria baicalensis extract, chitooligosaccharid, allyl isothiocyanate, sucrose-fatty acid ester, and oligosaccharide). The lowest minimal inhibitory concentrations (MIC) were 10 ppm for oligosaccharide and sucrose-fatty acid ester against S. cerevisiae and Z. bailii, 10 ppm for allyl isothiocynate against P. membranaefaciens and C. albican, and 10 ppm for allyl isothiocynate and oligosaccharide against P. anomala . No growth were observed for five kinds of yeasts in functional beverages containing sodium benzonate at concentration of 0.015% or higher. The resistance of S. cerevisiae, Z. bailii, and P. Anomala against natural antimicrobial agents was lower than those of P. membranaefaciens and C. albican. Allyl isothiocyanate, oligosaccharide, and sucrose- fatty acid ester showed the highest antimicrobial activities among the eight tested antimicrobials. These results can be applied to develop new natural antimicrobial agents to improve microbial quality of functional beverages.

Antifungal activity of seven natural antimicrobials, such as ε-polylysine, extract of Yucca shidigera, TLS (vitamin B1 derivative), BMB-FS, chitooligosaccharide, KDSP 001, and KDSP 002 were investigated for their applications in functional beverage. Five fungi including Aspergillus niger, Penicillium citrinum, Rhizopus oryzae, Fusarium moniliforme, and Mucor rouxii were applied as test fungi and mininum inhibitory concentrations (MICs) of antimicrobials were examined. TLS exhibited the strongst antifungal activity among tested antimicrobials and the growth of all fungi was inibited at 100 ppm. The antifungal activity of BMB-FS appeared different. The growth of Fusarium moniliforme was inhibited by BMB-FS at 100 ppm, but it exhibit antifungal activity on P. citrinum, and M. rouxii at 1000ppm. MICs of TLS appeared to be 60 ppm for four test fungi except A. niger (100 ppm). These results indicate the possible usage of TLS and BMB-FS as natural antimicrobials in functional beverage.

The purpose of this study was to investigate the antimicrobial activities of natural antimicrobials (10 formulas, F1~F10) against yeasts in functional beverages. The growth rates of yeasts were different with the ten different natural antimicrobial formulas tested. Yeasts grew for 14 days and the antimicrobial effect was observed between 14 and 18 days. Levels of S. cerevisiae, Z. bailii, and P. membranaefaciens were reduced to the limit of detection (ND) < 10 CFU/mL) after 28 days. Resistance against the antimicrobial effect was greatest for P. membranaefaciens, which grew to a level of 0.12~1.48 log10 CFU/mL after 14 days and was reduced to a level of 1.61~3.55 log10 CFU/mL after 28 days. The resistance of C. albicans was also high with a growth level of 0.13~1.28 log10 CFU/mL after 14 days and reduction to 1.51~5.30 log10 CFU/mL after 28 days. The antimicrobial effect of F10 was strongest for P. membranaefaciens. Every treatment reduced the microbial levels to 2.68~5.62 log10 CFU/mL after 6 months. F2, F4, F5, F6, and F10 reduced the C. albicans level to ND after 28 days while F1, F3, F8, and F9 reduced yeasts to the ND level after 6 months. The antimicrobial activities observed here will be useful for development of natural antimicrobials.

녹차 잎 1.5 g에 증류수 100 mL을 가하여 75℃에서 5분 동안 추출하여 녹차 음료를 제조하였다. 저장 조건을 달리하여 A 녹차 음료 (빛을 인위적으로 차단하지 않음, 자연 상태의 대기 조건, 비타민 C 무첨가), B 녹차 음료 (빛을 알루미늄 호일로 감싸서 차단함, 질소를 불어넣어 자연 대기를 질소로 치환하였음, 비타민 C 30 mg/100 mL 첨가), C 녹차 음료 (빛을 알루미늄 호일로 감싸서 차단함, 질소를 불어넣어 자연 대기를 질소로 치환하였음, 비타민 C 무첨가)로 구분하여 4℃에서 28일간 저장한 후, 유용 성분을 분석하였다. 총페놀함량의 경우 저장 초기에 비하여 저장 28일 후 A 녹차 음료는 71.50%, B 녹차 음료는 73.88%, C 녹차 음료는 75.07%로 감소하였다. DPPH 라디컬 소거능은 저장 초기에 비하여 A 녹차 음료는 87.87%, B 녹차 음료는 92.93%, C 녹차 음료는 88.39%이었다. 녹차의 유용 카테킨인 epigallocatechin gallate (EGCG)는 28일간 저장 후에 A 녹차 음료는 130.61%, B 녹차 음료는 136.47%, C 녹차 음료는 4.34%이었다. 이상의 결과는 녹차의 저장 조건이 녹차 음료의 성분에 영향을 미치며, 빛, 질소 치환, 비타민 C가 매우 중요함을 시사하였다.

Patulin, a mycotoxin mainly produced by Penicillium and Aspergillus, is found in various foods. In the present, a maximum acceptable level for patulin is established at 50 μg/kg(ppb) in apple juices and apple concentrates in Korea. But patulin may be detected in foods produced with other fruits. In the present study, patulin contamination was analyzed in 520 samples of fruit juices and beverages, and 50 samples of fruit juice concentrates. High performance liquid chromatography(HPLC) was applied to quantitatively analyze patulin levels in samples and liquid chromatography-mass spectrometry(LC/MS/MS) was used to remove false positive results. The results showed that three samples of 520 fruit juices and beverages and five samples of 50 fruit juice concentrates were contaminated by patulin, 9.8 - 18.0 μg/kg and 4.7 - 18.2 μg/kg respectively. Contaminated samples were produced with apple, orange or pear. This indicates that it is necessary to extend the regulatory range of patulin. In the other hands, the present study confirmed the effectiveness of LC/MS/MS analytical method to remove false positive results.

최근 양파를 많이 섭취한 사람들의 경우 암, 심혈관계질환 등 노화관련 질환의 발생률이 현저히 떨어진다는 보고가 잇따르면서 건강기능성 소재로서 양파에 대한 소비자들이 인식이 매우 높아져 있다. 현재 양파의 국내 자급률은 100%에 이르기 때문에 양파를 소재로 한 다양한 기능성식품의 개발은 국내에서 생산되는 농산물을 이용한 건강식품시장의 활성화에 밑거름이 될 것이며, 국내외적으로 우리나라 농산물의 고부가가치화를 추구하는데 크게 기여할 것이다. 따라서 본 연구에서는 우수한 기능성 소재로 입증된 양파를 이용하여 음료의 제조공정 개발 및 최적화시키고자 하였으며, 발효 양파 착즙액에 사과즙, 당, 산, 올리고당 등을 첨가하여 유산 발효 양파음료 시제품을 제조하여 1차, 2차 및 3차 관능평가를 실시한 결과 최종적으로 가장 우수한 배합은 양파 착즙액 27%, 사과즙 27%, 액상 과당 3.8%, β-cyclodextrin 1.86%, 올리고당 0.9%, 사과향 0.2%, 정제수 39.25%였다.

This study investigated elementary school students' perception, preferences, and intake of Korean traditional foods, focusing specifically on kimchi, tteok (rice cake), and eumcheong (beverage) varieties; and compared them by gender, living with grandparents, mother's occupation, and meal preparation by the grandmother. The subjects were 287 6th grade elementary school students in Busan. The results were as follows: 80% of children were interested in Korean traditional foods. 40% believed that their intake of Korean traditional foods was decreasing because these foods were not palatable to them. The majority of them, however, said they would continue to eat Korean traditional foods as they had done (54.7%) or eat more than before (36.6%) in the future. The children thought that Korean traditional foods were rich in nutrition and good for their health. The children had the highest preference for Baechu-kimchi among varieties of kimchi, and they had high preferences for Songpyeon, Galaitteok, and Injulmi. They had high preferences for Sikhye, citron tea, and adlai tea. Over 80% reported consuming Baechu-kimchi and Kkakdugi three to four times per week. They had eaten Injulmi the most frequently among the tteoks, while over 80% had eaten the other types of tteok only once or twice per month. Adlai tea, citron tea, and Sikhye were drunk more than once per week. In general, we noted no significant differences in the children's perceptions, preferences, and intake of Korean traditional foods by gender, living with grandparents, mother's occupation, and meal preparation by grandmother, with the exception of several items. The students had a very positive perception of Korean traditional foods. They had higher preferences for and had more frequently consumed the more familiar Korean traditional foods. It is therefore suggested that if the children had opportunities to experience Korean traditional foods more frequently and variously at home or in restaurants, they would appreciate Korean traditional foods even more, and develop higher preferences for these foods.