This research aims to identify the possibility of developing A horizon resources that can be used for construction and civil engineering work. As such, the utility of A horizon resources was examined by establishing planting ground through a mixture of soil layers and by analyzing the growth and development of Pinus densiflora. The physicochemical and physical properties of the soil were as follows: the A horizon was sandy clay loam, B horizon was sandy loam, and the mixture of two layers appeared as sandy loam, which was identical to the B horizon. The experimental groups did not show any significant difference in their physical properties of porosity and degree of water-stable aggregates. With regards to chemical properties, the A horizon as well as the mixture of A and B horizon showed acidity while the B horizon showed alkalinity. The figures of organic matter, total nitrogen, available phosphate, and replaceable potassium were greater as the A horizon content increased, whereas the figures of replaceable calcium, replaceable magnesium, and conductivity increased as the A horizon content decreased. As a result of the growth and development of Pinus densiflora in each planting ground, the final survival rates were all above 100%. However, the tree height and the rate of growth for the diameter of root were higher in the order of A horizon > A horizon + B horizon > B horizon,indicating that the increased A horizon content is related to the growth and development of Pinus densiflora. The treatment of soil with improvement agents, used to recover the functions of in-situ soil showing poor growth and development, did not have a clear impact on the soil texture and porosity. However, the degree of water-stable aggregates increased significantly when using O horizon as the soil improvement agent among the types of in-situ soil. In contrast, all items related to the chemical properties showed significant differences following the treatment by soil improvement agents. The survival rate according to the treatment of soil improvement agents for the growth and development of Pinus densiflora was higher in the order of organic horizon = no treatment > compound fertilizer > organic fertilizer + compound fertilizer > organic fertilizer; this result was statistically significant with a marginal significance value of the log-rank test(p < 0.05).

Antioxidant activity and inhibitory effect on oxidative DNA damage of ethyl acetate fractions extracted from Cone of Red Pine (Pinus densiflora) were investigated to find utilization of Cone, by-product of Red Pine, thrown out after berry shatter, as a new natural plant resource. Cone from P. densiflora was extracted with methanol (MeOH) and separated to petroleum ether, ethyl acetate and water fraction. Among them, ethyl acetate fraction was used. The antioxidant activity was conducted by the 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, 2, 2'-Azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) radical scavenging assay, Fe 2+ chelating assay and reducing power assay. The inhibitory effect on oxidative DNA damage was determined by DNA cleavage assay using φX-174 RF I plasmid. The results of DPPH and ABTS radical scavenging activity at 200 ㎍/㎖ of extracts were 86.50% and 95.80% respectively, which were similar figures compared with L-ascorbic acid as control. Fe 2+ chelating activity was 77.96% and reducing power was 0.77 at 200 ㎍/㎖. Total phenolic component was 27.29±0.3 ㎎/g and Vitamin C content was 1.84±0.1 ㎎/g. Also ethyl acetate fraction from Cone has inhibitory effect, using φX-174 RF I plasmid on DNA cleavage assay. In conclusion, Cone, by-product of P. densiflora, showed high antioxidant activity and inhibitory effect on oxidative DNA damage. Therefore this study suggests Cone, useless by-product, can be developed as a new natural plant resource with lots of utilization such as an effective antioxidant, natural medicine, food, cosmetics and so on.

In this study, the antioxidative activity and functional food activities of water and ethanol extracts from Pinus densiflora root were examined. It was more effective to use ethanol than water when extracting phenolic compounds. The extracted phenolic compounds from Pinus densiflora root for biological activities were examined. The phenolic compounds extracted with water and 80% EtOH were 1.86±0.04 mg/g and 6.85±0.16 mg/g, respectively. DPPH free radical scavenging activity of water and EtOH were each 86% and 85% at 100 μg/mL phenolics, respectively. ABTS radical decolorization activity was 48% in water and 68% in EtOH at 200 μg/mL. Antioxidant Protection Factor (PF) were 1.74 PF in water and 1.96 PF in EtOH at 50 μg/mL. TBARs of water and EtOH were 93% and 98%, respectively at 100 μg/mL. The inhibition activity on xanthine oxidase was 83.7% in water extracts and 79.6% in ethanol extracts. Inhibition on xanthine oxidase of water and ethanol extracts showed a higher inhibition effect than allopurinol. The inhibition activity on α-glucosidase was 14.8% in water extracts and 91.6% in ethanol extracts. The result suggests that P. densiflora root extracts may be useful as as functional food material.

The effect of seasonality is one of the most significant external sources of variation affecting cambial activity and the development of newly divided cells, and therefore influencing stem growth of trees. Here, we investigated changes in the seasonal concentrations of metabolites of current-year stem tissues in 6-year-old Pinus densiflora at June, August, and October. 76, 75, and 78 metabolites were assigned at June, August, and October by GC/MS. Among these compounds, 55 metabolites were commonly found in all three times, and they were divided into six groups according to the variation of concentrations in each times. Among 56 metabolites, the concentrations of three inositol-methylated derivatives, myo-inositol, ononitol, and pinitol in current-year stem tissues at August were significantly correlated with the heights of nursery-grown trees. Furthermore, we found that such metabolites were significantly correlated with stem diameter at 27 years for two consecutive years. Therefore we suggest that seasonal differences in the contents of inositol derivatives may explain much of the natural variation seen for tree stem size in even-aged pine forests. And these have the potential as metabolic markers of inherently rapidly growing trees in the early selection of those conifer families.

소나무의 유전다양성과 유전구조를 추정하기 위해 9개의 ESTP 표지를 13개 소나무 집단에 적용하였다. 소나무 집단의 유전다양성은 관찰된 대립유전자 수(A)가 2.2개, 유효 대립유 전자 수(Ae)가 1.8개, 다형적 유전자좌 비율(P)이 98.8%, 이형접 합도 관찰치(Ho)가 0.391, 이형접합도 기대치(He)가 0.402로 나 타났다. 안강과 강릉 집단을 제외한 11개 집단이 하디-바인베르 그 평형을 만족하였다. 집단간 유전분화도(FST)는 0.057으로, 동위효소나 nSSR 표지분석 결과보다 강하게 나타났다. 군집분 석에서 집단의 유전적 거리와 지리적 분포간에 뚜렷한 연관성 은 확인할 수 없었으며, 집단의 유전분화와 지리적 인접성도 상 관이 없는 것으로 나타났다(Mantel 검증, r = 0.017, P = 0.344). 유전자좌에 대한 FST-outlier 분석을 실시한 결과, 빈도 주의 방법에서는 FST 값이 신뢰하한 이하인 3개 유전자좌와 신 뢰상한 이상인 3개 유전자좌가 특이값으로 추정되었고, 베이즈 방법에서는 3개 유전자좌들만 특이값으로 확인되었다. 두 방법 에서 공히 특이값으로 판정된 3개 유전자좌(sams2+AluⅠ, sams2+RsaⅠ, PtNCS_p14A9+HaeⅢ)중 sams2 표지에서 유래 된 2개 유전자좌는 balancing selection의 영향을 받는 것으로 추정되었다.

부안지역 소나무 집단의 화분유동과 교배양식 모수를 추정 하기 위하여 7개 microsatellite 표지로 모수, 주변 성목 및 종자 에 대한 유전변이를 분석하였다. 이형접합도 기대치(He)와 근 교계수(F)는 각각 모수에서 0.614과 0.018, 종자에서 0.624과 0.087이며, 각 세대간에 차이는 없었다(P ＞ 0.05). MLTR로 추 정한 타가교배율(tm)은 0.967이며, 양친간 근연계수(tm-t s)는 0.057, 부계상관(rp)은 0.012로 나타났다. 기존에 보고된 소나 무의 동위효소 분석 결과에 비하여 타가교배율은 높고 근친교 배 및 부계상관은 낮았으나, microsatellite 표지를 이용한 소나 무류의 결과들과는 유사하였다. TwoGener로 추정한 최적 화분 비산 모델은 유효밀도(d = 220 trees/ha)를 가정한 정규확산모 델로 판명되었으며, 평균 화분비산거리(δ )는 11.42 m로 계산되 었다. 화분원 유전적 분화(Φft)는 0.021이며, Mental 검증에서 모수간 지리적 거리와 화분원의 유전적 분화는 상관성이 없는 것으로 나타났다(r = -0.141, P ＞ 0.05). 부안지역 소나무 집단 은 대부분의 화분이 가까운 거리에서 공급되지만, 화분수의 유 전다양성이 높고 화분원의 유전적 차이가 작은 상태로 추정된 다. 이러한 조건에서 완전한 임의교배가 이루어지기 때문에 종 자의 유전자형이 다양하며 세대간 유전변이의 감소가 없는 것 으로 사료된다.

한국구비문학대계 49편, 한국구전설화집 85편, 삼국유사 수록 14편에 수록된 148편의 수목설화에 나타난 나무 종류와 한국인의 수목관을 조사하였다. 그 결과를 정리하면 다음과 같다. 첫째, 설화에 소나무가 가장 많이 나타나 소나무는 우리 민족의 삶과 정서에 밀접한 관련을 맺고 있는 나무임을 알 수 있다. 둘째, 은행나무, 느티나무, 대나무, 버드나무, 유실수 등과 같이 생활과 밀접히 관련 있는 생활권주변의 친숙한 나무들이 주로 등장하였다. 셋째, 활엽수가 침엽수 보다 훨씬 많이 나타나 활엽수종들이 민중들에게 보다 친숙했음을 알 수있다. 넷째, 설화 속 나무의 형상을 통해나무는 생명의 원천이자 세상의 이치를 깨닫게 하는 신성한 능력을 지닌 주술적 상징물로서, 나무를 인격화하여 인간과 교감 및 소통의 대상으로서, 나무의 강한 생명력과 번식력, 재생력을 통해 풍요, 다산, 다복의 상징물로서 인식되고 공유했음을 확인하였다. 본 연구는 한국인의 나무나 숲에 대한 태도 또는 인식을 직접적으로 남긴 문헌이나 기록이 미비한 상황에서 수목에 대한 관념이나 견해를 설화를 통해 간접적으로 유추했다는 데 의의가 있다. 또한 현재 산림치유나 원예치료 등 자연물을 대상으로 국민건강을 도모하기 위한 프로그램 개발의 시도가 활발히 이뤄지고 있는 반면, 현장에 적용할 프로그래밍 소재 발굴에 어려움을 호소하는 경우가 많다. 이와 같은 상황에서 우리 전통문화에 등장하는 소재들은 참가자들에게 좀 더 의미 있고, 유익한가치를 제공하는 기회가 될 수 있을 것이다. 따라서 본 연구에서 도출된 결과들이 향후 산림문화자원을 활용한 프로그램 개발에 기초자료로써 유용하게 활용될 수 있기를 기대해 본다.

This study was conducted to figure out the differences in physiological responses, e.g. growth, photosynthetic activity and water potential, and photoinhibition tolerance in photosystem between superior and inferior families of Pinus densiflora under drought condition. Superior which is KW85 and inferior which is KW40 families were selected using progeny test results of height growth. In 2007, seeds were collected from seed orchard. In 2008, seedlings were produced and cultivated, and from April 2009 drought treatment was started with shading treatment and plants were harvested in Sept. 2009. There was no significant difference in height growth between families. In case of leaf water potential, KW85 showed higher water potential under drought condition. But there was no significant difference in drought with shading treatment. There was no difference in photosynthetic rate but stomatal conductance and transpiration rate of KW85 showed lower value than KW40. So water use efficiency of KW85 showed higher value in every treatment. Non-photochemical quenching of KW40 showed higher value in drought treatment, but there was no significant difference in control and drought with shading treatment. Xanthophyll cycle pool size of KW85 showed higher value in drought treatment, but in drought with shading treatment KW40 showed higher value. Selected superior family showed higher drought tolerance according to water use efficiency, and it also has effective non-photochemical quenching ability. In contrast inferior family respond more sensitively in photoinhibition under drought condition.

2012년 11월 카타르 도하에서 열린 유엔 기후변화 당사국총회 COP18 장관급회의에서는 올해 말 그 시한이 종료되는 도쿄의정서를 대신할 ‘도하합의’를 도출하였다. 2020년까지 교토의정서를 연장하고, 모든 선진국과 개발도상국이 같이 적용받는 구속력 있는 온실가스 감축안을 2015년까지 만들기 위한 기반을 조성하자는 내용이 주요골자이다. 그간 우리나라는 2010년을 기준으로 세계 이산화탄소 배출량의 1.7%를 차지하고 세계에서 8번째로 이산화탄소를 많이 배출하는 국가가 되었다. 이에 2015년 합의될 온실가스 규제에서 우리도 상당부분 배출량감소를 요구받을 전망이다. 온실가스를 발생시키는 다양한 경로 중의 하나는 산불발생으로 생성되는 산림의 바이오매스 연소이다. 교토의정서 이후 각국은 산불발생으로 인해 유발되는 온실가스인 이산화탄소와 비이산화탄소의 정량적이고 과학적인 방법을 연구 개발하고 있다. IPCC(International Panel on Climate Change)는 접근방법과 기본자료를 이용하여 배출량을 분석하는 수준1과, 국가고유활동 자료나 배출계수를 이용하여 배출량을 분석하는 수준 2, 국가 고유자료와 방법 모두를 이용하는 수준3을 제시하는데 우리나라도 수준 2나 3의 단계로 진입하는 다각도의 노력을 벌이고 있다. 본 연구는 우리나라 고유 배출계수 개발을 위해, 수관화의 위험이 높은 소나무의 바이오매스 연소에 따른 CO2/non-CO2 배출량을 정량적으로 추정하기 위한 작업의 하나로 이루어졌다. 실험방법은 삼척소재 소나무를 대상으로 영급별로 생엽과 굵기 10mm이하의 가지를 채취하여 콘칼로리미터와 NDIR분석기로 연소 시 배출되는 CO2, CO, NO, CH4, N2O의 배출량을 분석하였다. 실험조건은 10cm×10cm×5cm의 방형틀을 채우는 생엽과 가지부분을 건조기에 건조하여 더 이상 중량변화가 없을 때까지 실험하여 얻은 값을 사용하였으며, 결과값은 3회 측정한 평균값이다.

This study was carried out to investigate the distribution and their change of Pinus densiflora forests by climate change in Mt. Hallasan. The results showed that the areas of P. densiflora forests of Mt. Hallasan varied by region, with a total area of 1,324.3 ha, concentrated mostly in the region 1,000 m - 1,400 m above sea level. The temperate coniferous forest zone are distributed in the upper part of temperate forest zone composed of deciduous broad-leaved trees. Most of the P. densiflora forests in the lower parts were found not to be spreading because they are located close to the deciduous broad-leaved trees. However, the P. densiflora forests in the Sajebi and Pyeonggwe regions composed of the grasslands and shrub forests were found to be spreading. In addition, the altitude of the P. densiflora forests distribution increased by about 50 m and 90 m in the Sajebi and Pyeonggwe regions, respectively. The spread rate is expected to become faster than in the past due to the rate of climate change. The structure of the vegetation in Mt. Hallasan and the changes in the vegetation due to various factors need to be investigated from a long-term point of view.

신속하게 생장하는 소나무의 조기 선발인자를 구명하고자 32년생 소나무의 풍매차대 검정결과로 얻어진 재적지수를 기준으로 상, 중, 하로 구분하여 선발된 12가계의 생장특성과 동일한 가계의 유묘 생장특성과의 상관관계를 분석하였다. 선발된 12가계의 종자 특성과 동일 가계의 성목 생장특성과는 상관관계가 없었으며 다만 소나무 유묘의 초기 생장과는 관련이 있는 것으로 나타났다. 가계별 1년생의 유묘 건물중과 성목의 재적지수(32년생)와는 1%의 유의적 정의 상관

This study was carried out in each three study areas of Pinus densiflora community and Quercus mongolica community from March 5th, 2008 to October 15th, 2010 to analyze the relationship between seed bank and the actual vegetation of the lower layer. Based on the relationship between the lower layer of actual vegetation and the germination of seed bank, all of three study areas, the similarity of the actual vegetation of the lower layer and seed bank were high in Plot 1 (84.62%) and Plot 3 (89.91%). As for Quercus mongolica community, the similarity was high between the actual vegetation of the lower layer and seed bank in Plot 4 (82.24%) and Plot 6 (89.47%). Especially, the germination of the pine seed banks in the Pinus densiflora community compared to other tree species appeared in all. In Quercus mongolica community, Quercus mongolica did not appear among the seeds germinated in the seek bank, but the other tree species constituting the under layer of the community. In case of the restoration based on the actual vegetation, it is desirable to sue the lower layer of vegetation as the model for the making of its alternatives for restoration works of the species.

Three-year-old seedlings of susceptible Pinus densiflora and resistant Pinus x rigitaeda were each inoculated with the pinewood nematode, Bursaphelenchus xylophilus, to compare disease development. Needle dehydration was evident on seedlings of P. densiflora by 20 days after inoculation, 10 days earlier than this symptom was observed on P. × rigitaeda. Xylem drying was more frequent in seedlings of P. densiflora than in that of P. × rigitaeda between 20 and 60 days after inoculation. No significant differences were found between P. densiflora and P. × rigitaeda for stem water content or for stem and leaf relative water content in current-year branches after nematode inoculation, but the average number of B. xylophilus recovered from stems differed significantly between the two groups. The number of B. xylophilus recovered from stems was negatively correlated with the stem water content and with stem and leaf relative water content. By the time the experiment was terminated at 60 days after inoculation, all 3 of the last group of P. densiflora seedlings had died, but 2 of the 3 remaining P. × rigitaeda hybrid seedlings were still alive. Additional studies are needed to further explore the specific mechanisms preventing nematode multiplication in the seedlings of resistant P. × rigitaeda.

소나무 수형목 풍매차대를 대상으로 수고생장에 대하여 일반조합능력을 평가하고자 본 연구를 수행하였다. 본 연구에 사용된 가계는 총 232가계로 각 수령별 10년생 230가계, 20년생 184가계, 30년생 122가계를 대상으로, 차대검정림에서의 수고 생장 조사 자료를 이용하였다. 차대검정림은 1975년부터 조성하기 시작하여 1987년까지 모두 7회에 걸쳐 1~4 지역에 조성하였다. 이러한 조성 년도, 지역의 차이를 표준화를 통하여 균일한 수치로 보정하여

We described the associated variation in site index of Pinus densiflora for. erecta in responses to site variables of soil properties. Additionally, this study was conducted to estimate site index in unstocked land by the physico-chemical properties derived from stocked forest soil and to investigate the effects of the physico-chemical properties by soil layers on site index of surveyed area, totally 77 sites in central Korea. At each site, there were surveyed 14 items of the physico-chemical properties, namely, content of sand, silt and clay, soil pH, organic matter, total nitrogen, available P2O5, cation exchange capacity, exchangeable K+, Na+, Ca++, Mg++, and base saturation. The multiple regression equations using stepwise regression method were applied to this study. In the degree of contribution of the variables to site index by soil horizons, the highly effective variables were Base Sat., Exch. Ca++, T.N., Exch. Mg++, and C.E.C. in A horizon and Base Sat., Exch. Mg++, C.E.C., Soil pH, and O.M. in B horizon of P. densiflora for. erecta stands. As a result, it was concluded that the regressive prediction induced by significant soil site factors by forest soil horizons had useful guidance for finding out the best suitable area especially in unstocked land and further more for establishing successful stands of this species.

We conducted this study to compare physiological response according to growing conditions between superior and inferior family of Pinus densiflora. In 1981, open-pollinated (OP) progenies of P. densiflora were planted in Chuncheon (CC) and Naju (NJ). We selected KW2 as a superior family and KW23 as a inferior family based on volume index among 30 OP progenies. We calculated general combining ability of each 30 OP progenies in each sites, and compared family growth rank. We collected needles of selected families in CC and NJ, and analyzed chlorophyll content, nitrated reductase (NR) activity, nitrogen content, and superoxide dismutase (SOD) acitivity. In CC, chlorophyll content and nitrogen content were more in needles of KW2 than those of KW23. In NJ, KW2 showed higher NR activity than KW23, and according to site, families in CC showed higher NR activity than those in NJ. SOD activities of both families were higher in NJ, and KW23 showed higher activity than KW2 in both sites. Consequently, inconsistency of the growth performance of two families was caused by different genetic and physiological responses.

소나무 풍매차대의 유시와 성숙기 이후의 생장 패턴을 비교하고, 유전인자와 환경인자간의 상호작용을 평가하고자 본 연구를 수행하였다. 본 연구에 사용된 종자는 1978년에 강원도, 경기도, 충청북도에서 선발된 수형목으로부터 채취되었다. 차대검정림은 1981년에 강원 춘천, 충남 공주, 전남 나주 등 3 지역에 조성되었으며, 총 30가계를 식재하였다. 식재 후 일정 시기별로 수고와 흉고직경을 측정하였으며, 이로부터 재적지수를 산출하였다. 수고,흉고직경 및 재

The pine trees have been existing in Korean Peninsula since about 7,000 years ago. It is the tree which has been always present in Korean's life and it is the tree which has a potential to become a true identity of Korea's Nature. In This study, the characteristics of pine(Pinus densiflora Sieb. et Zucc.) forest in the "True-View landscape paintings" during the late chosun dynasty were investigated according to the 9-categorization. The result of the study are as follows: 1452 pine tree forests samples are collected from 179 "True-View landscape paintings". The 9 classifications are following; Mountain top, saddle os mountain, foot of hill, Ridge, Valley, Slope, Rock, Soil, Waterside. In the case of the topography, 64.3% of the pine forests are drawn in the ridges. The forests are mainly located on the tops and the saddles of mountains. The current ecological findings also show that the ridges are the most suitable sites for pine trees. the pine forests drawn in the valley are frequently in the foot of mountains and the rock. The slopes(135) and the valleys(267) are mainly located foot of hill.

해송×소나무 인공교배 차대들의 침엽 및 생장특성을 조사하였다. 해송×소나무 교잡종 3, 5, 8년생 수고생장은 조합간에 많은 차이가 있었다. 해송(충남 4)×소나무(강원 29) 조합 3, 5, 8년생의 수고생장이 110, 172, 234cm로 전 조합 평균보다 8%, 9%, 11% 우수하였으며, 교배 모수인 해송 수형목 충남4호와 화분수인 소나무 수형목 경북 5호 보다도 각각 46%, 61% 우수한 생장을 하여 잡종강세 현상으로 생각된다. 해송×소나무 침엽의 수지구지수는 0.71~0.89로서 교배모수인 해송을 더 많이 닮았다.

우리나라의 대표적 향토수종인 소나무 침엽의 지리적 변이를 알아보기 위하여 남한 전 지역을 대상으로 위도와 경도를 30' 간격으로 교차되는 35집단을 선정하고 침엽을 채취하여 형태적 특성을 조사하였다. 침엽길이, 침엽폭, 기공열수, 기공밀도, 거치수, 수지구수가 집단간, 개체간에 많은 차이가 있었으며, 개체간의 차이가 집단간의 차이보다 더 컸다. 기공밀도는 남부지역의 집단들 보다는 북부지역의 집단들이 더 높았으며, 표고가 낮은 지역의 집단들 보다는 표고가 높은 지역의 집단들이 기공밀도가 더 높았다. 거치수는 북부지역의 집단들보다는 남부지역의 집단들이 더 많았으며, 표고가 높은지역의 집단들보다는 표고가 낮은 지역의 집단들이 더 많았다. 수지구 수는 해안에서 거리가 먼 내륙의 집단들 보다는 해안에서 거리가 가까운 집단들이 더 많았다. 그리고 수지구 지수는 동부지역의 집단들 보다는 서부지역의 집단들이 더 높았다. 침엽의 형태적 특성으로 유집분석 결과 3개 군으로 구분되었으며 제III군은 I, II군에 비하여 거치수는 적고, 기공 밀도가 높은 태백산맥 주위의 집단들이었다.