As a member of ectomycorrhizal fungi, Tricholoma matsutake has a symbiotic relationship with its host, Pinus densiflora. To cultivate T. matsutake artificially, the co-cultivation of T. matsutake mycelia and bacteria from shiro was introduced. In this study, bacteria were isolated from soil samples in Bonghwa-gun, and seven bacterial isolates (B22_7_B05, B22_7_B06, B22_7_B07, B22_7_B08, B22_7_B10, B22_7_B13, and B22_7_B14) promoted the growth of T. matsutake mycelia (147.48, 232.11, 266.72, 211.43, 175.17, 154.62, and 177.92%, respectively). Sequencing of the 16S rRNA region of the isolated bacteria was performed. B22_7_B05 and B22_7_B10 were identified as Bacillus toyonensis, B22_7_B06 and B22_7_B08 as Paenibacillus taichungensis, B22_7_B07 and B22_7_B14 as P. gorilla, and B22_7_B13 as P. odorifer. These bacterial isolates were associated with the shiro community and are expected to contribute to the cultivation of T. matsutake.

Airborne bacteria in mushroom growing environments are a potential risk of contamination in commercial mushroom production. Controlling contamination in mushroom farms requires understanding the bacterial ecology in the cultivation environment. This study was conducted to investigate the concentration and species diversity of floating bacteria in a thermophilic mushroom cultivation room. Temperature, humidity, temperature, humidity, and bacterial concentration measurements were performed in April and May 2022 for a Pleurotus ostreatus cultivation house, in July and August 2023 for a Pleurotus sajor-caju and a Agaricus blazei cultivation house, and in June, July and August 2023 for a Pleurotus pulmonarius, Pleurotus sajor-caju and Calocybe indica cultivation house. The airborne bacterial concentration was 5.27 × 103~105 CFU/m3, 3.81 × 102 ~1.37 × 103 CFU/m3, and 2.55 × 102 ~1.37 × 102 CFU/m3 in the three cultivation houses, respectively. A total of 23 genera and 37 species of airborne bacteria were isolated from the three mushroom cultivation houses. 12 genera and 18 species were identified from P. ostreatus cultivation house. Furthermore, 4 genera and 4 species were found from A. blazei and C. indica cultivation house. In addition, 11 genera and 18 species were isolated from P. pulmonarius, P. sajor-caju and C. indica cultivation house. Among the bacteria isolated, the Bacilli class was the most common, followed by Gammaproteobacteria. Among the 37 bacterial species, it was determined that Bacillus cereus, B. licheniformis, Cedecea neteri, Exiguobacterium acetylicum and Raoultella terrigena could negatively affect humans or foodstuff. Cedecea neteri is also known to cause diseases among mushrooms.

Rapid and accurate detection of pathogenic bacteria is crucial for various applications, including public health and food safety. However, existing bacteria detection techniques have several drawbacks as they are inconvenient and require time-consuming procedures and complex machinery. Recently, the precision and versatility of CRISPR/Cas system has been leveraged to design biosensors that offer a more efficient and accurate approach to bacterial detection compared to the existing techniques. Significant research has been focused on developing biosensors based on the CRISPR/Cas system which has shown promise in efficiently detecting pathogenic bacteria or virus. In this review, we present a biosensor based on the CRISPR/Cas system that has been specifically developed to overcome these limitations and detect different pathogenic bacteria effectively including Vibrio parahaemolyticus, Salmonella, E. coli O157:H7, and Listeria monocytogenes. This biosensor takes advantage of the CRISPR/Cas system's precision and versatility for more efficiently accurately detecting bacteria compared to the previous techniques. The biosensor has potential to enhance public health and ensure food safety as the biosensor’s design can revolutionize method of detecting pathogenic bacteria. It provides a rapid and reliable method for identifying harmful bacteria and it can aid in early intervention and preventive measures, mitigating the risk of bacterial outbreaks and their associated consequences. Further research and development in this area will lead to development of even more advanced biosensors capable of detecting an even broader range of bacterial pathogens, thereby significantly benefiting various industries and helping in safeguard human health

ATP luminescence measurements (using Relative Light Units, RLU) has been used to assess the levels of bacterial contamination on the surfaces of various materials. However, not much is known about their suitability in assessing bacterial contamination on paper surfaces. This study was conducted to evaluate the feasibility of using ATP luminometers in measuring levels of bacteria contamination on paper surfaces. The three ATP luminometers studied were Clean-Q, smart PD-30, and 3M™ Clean-Trace™ LM1 manufactured by different companies. There were some differences in RLU results among the three ATP luminometers when they were tested with different concentrations of Micrococcus luteus cell suspension. 106 - 107 cells were required in order to effectively detect Bacillus subtilis, Escherichia coli, and Micrococcus luteus on the surfaces of A4 printing sheets (100 cm2) when using the three ATP luminometers. The sizes and physical properties of surface areas varied slightly among the swabs used for the three ATP luminometers. Concentration-specific measurements (RLU) of M. luteus taken from the surfaces of six kinds of paper (fine print paper, medium print paper, ground paper, newsprint paper, practice paper, tracing paper) were possible using the smart PD-30 and LM1 ATP luminometers. ATP detection values of M. luteus varied among the six types of paper. The highest ATP detection values were found on the surfaces of tracing paper. If the RLU value is recorded at the level of 1000, this could indicate a very high bacterial contamination level of 105 to 106 CFU/4 cm2.

본 연구는 인체 및 온혈 동물의 기회감염 병원세균으로 알려진 Pseudomonas oryzihabitans에 의한 양파 구에 발생한 중심 흑화병의 첫 보고이다. 본 연구의 목적은 양파 구 흑화병을 일으키는 원인 병원체를 분리 및 동정하고 미생물학적 특성을 조사하는데 있다. 2021년 국내 양파 구에서 소비자 혐오감을 유발할 수 있는 세균 흑화병이 관찰되었다. 흑갈색 색소가 침착된 조직과 흑색 색소 과육 조직에서 그람음성, 비포자형성, 막대형 pseudomonad들이 분리되었다. 병원성 검정을 통해 형성된 인공 감염후 발생한 증상은 자연 감염과 같았고 멸균 증류수를 처리한 음성 대조구에서는 흑화병이 발견되지 않았다. 병원성 검정, API (analytical profile index) 시험, MALDI-TOF MS (Whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry), 16S rRNA 유전자 영역 염기서열분석 및 유전적 유연관계분석을 통해 원인 병원체를 Pseudomonas oryzihabitans로 동정하였다. 대규모 포장 또는 수확후병으로 인한 양파 피해사례가 보고된 적은 없지만, 본 연구에서 얻은 결과는 P. oryzihabitans가 잠재적으로 양파의 글로벌 생산 및 운송과 식품 안전을 위협할 수 있음을 시사한다. 또한 인체 기회감염 병원체이기 때문에 양파로부터 언제든지 인체로 감염될 수 있는 위험성을 갖고 있다고 볼 수 있다.

Fluorescent probe were used to evaluate the effects of catechin on the structural parameters (annular lipid fluidity, transbilayer lateral and rotational mobility and protein clustering) of the Porphyromonas gingivalis outer membrane (OPGs). An experimental procedure was used on the basis of selective quenching of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1,3-di(1-pyrenyl)propane (Py-3-Py) by trinitrophenyl groups and radiationless energy transfer from the tryptophans of membrane proteins to Py-3-Py and DPH. Catechin increased the bulk lateral and rotational mobility, annular lipid fluidity of OPGs lipid bilayers, and had greater fluidizing efficacy on the outer monolayer than the inner monolayer. It also caused membrane proteins to cluster. Based on these effects of catechin on OPGs, the antibacterial and antiviral actions of catechin can be partially explained.

Bis(2-ethylhexyl) phthalate (DEHP) is one of the plasticizers used in the polyvinyl chloride (PVC) industry. It is known to be easily released into the environment. In this study, we investigated effects of DEHP on growth, metabolic pathway, and virulence gene expression in soil-borne bacterial plant pathogen, Pectobacterium carotovorum SCC1 using in vitro assays. As a result, DEHP at 20 μg mL-1 did not affect the growth, cell membrane permeability, or ATPase activity of P. carotovorum SCC1. However, it decreased succinyl-CoA synthase (SCS) activity in the tricarboxylic acid (TCA) cycle. Relative expression levels of virulence genes encoding pectate lyase and pectin were differentially influenced by DEHP treatment. These results suggest that biological characteristics of P. carotovorum might be influenced by DEHP in soil.

This study was carried out to investigate how airborne bacteria are distributed under different temperature conditions while cultivating oyster mushrooms by setting the indoor temperature of the cultivation room to 10°C, 15°C, 20°C, 25°C, and 30°C. The surveys were conducted in April and May, respectively. Airborne bacterial concentrations were distributed in the range of 1.61 × 102 ~ 3.67 × 102 CFU/m3 in April and 5.47 × 102 ~ 7 × 103 CFU/ m3 in May. In May, the indoor air quality maintenance standard (8.0 × 102 CFU/m3) was exceeded in the 10°C, 20°C, and 25°C cultivation rooms. Bacterial concentrations increased significantly in May compared to April. Bacterial concentrations were different between the cultivation rooms at different temperatures. The difference was more pronounced in May than in April. A total of 15 genera and 20 species were isolated from the indoor air of the oyster mushroom cultivation rooms. Overall, it was most abundant in Actinomycetia. Among the species identified, Agrobacterium radiobacter, Brevundimonas vesicularis, Kocuria palustris, K. salsicia, Lysinibacillus fusiformis, and Sphingobacterium siyangense are known to affect human health. This is the first report of airborne bacteria in cultivation rooms at different temperatures used for oyster mushroom cultivation. The results of this study are expected to be used as basic data to understand the indoor environment of thermophilic mushroom cultivation facilities.

Grapefruit extract rich in functional substances adjusting pH 5.0 and adding sucrose (final concentration, 2%) was fermented by Leuconostoc mesenteroides CJNU 0147 strain, consequently prepared a fermented grapefruit extract containing dextran. As a result of analyzing the growth inhibitory effect on gut harmful bacterium Clostridium difficile strain using the prepared fermented grapefruit extract, the viable cell count was significantly reduced (p<0.05 vs. control for 0.5 brix as a final concentration; p<0.001 vs. control for 1.0 brix). On the other hand, the proliferative ability of the extract for Bifidobacterium breve strain, which is one of the species of the genus Bifidobacterium well known as human gut beneficial bacteria, was confirmed (p<0.001 vs. control for both 0.5 and 1.0 brix). These results indicate fermented grapefruit extract with Leu. mesenteroides CJNU 0147 strain inhibits the growth of gut harmful bacterium C. difficile and promotes the proliferation of beneficial bacterium B. breve and is expected to be used as a functional food material for gut health.

Porphyromonas gingivalis, a major pathogen of chronic periodontitis, colonizes in subgingival crevice and affects surrounding oral tissues, especially in periodontitis patients. Oral cancer mainly occurs in old-aged persons, and are exposed to the P. gingivalis, released from periodontitis, one of the most common inflammatory disease of oral cavity. Thus oral cancer cells may be infected with P. gingivalis, and its biologic behavior are autologously and/or heterogeneously modulated by altering gene expression. Exosomes which are derived from cells contain not only coding genes but also non-coding RNAs such as long non-coding RNAs, miRNA, and piRNAs. Here, to investigate the effect of P. gingivalis on oral cancer cells and to gain insight into the crosstalk between inflammatory signal from tumor microenvironment and oral cancer, we observed miRNA profiles of exosomes from P. gingivalis–infected oral cancer cells. Upregulation of 6 miRNAs, miR-203-3p, miR-6516-3p, miR-483-5p, miR-1275, miR-8485, and miR-19a-3p, were observed whereas 14 miRNAs including let-7a-3p, miR-106a-5p were downregulated. In addition, KEGG pathway analysis using the upregulated- and downregulated- miRNAs showed association with cell adhesion molecules pathway and ECM-receptor interaction pathway, respectively. These findings suggest that P. gingivalis could modulate biologic behavior of oral cancer cells through changes of exosomal miRNAs.

Distribution of airborne bacteria in the entire regions of South Korea was investigated and analyzed by region and type of multi-use facilities. At first, 10 public facilities were selected including general restaurant, retail store, public transport, retail market, apartment house, underground parking lot, financial institution, business facility, educational institution, and public toilet, which are located at the regions such as Seoul, Busan, Daejeon, Gwangju, Gyeonggi, Jeju, and Gangwon. The regional distribution of the floating bacteria was identified that Micrococcus sp. was highly prevalent in Seoul (21.5 percent). In Daejeon, Bacillus sp. was highly prevalent (12.4 percent). In Busan, Micrococcus sp. was highly prevalent (22.8 percent). In Gwangju, Bacillus sp. was 9.35%. In Gyeonggi, Micrococcus sp. was 13.7%, and in Gwangju and Jeju, Micrococcus sp. was 11.2 percent and 92%. All in all, Micrococcus sp. and Bacillus sp. were highly detected throughout the entire region and multi-use facilities. Next, whether or not these airborne bacteria could influence the health of people was examined using HaCat human skin cell line which is human epithermal Karatinocytes related to allergic dermatitis. Among these isolated microorganisms, the HaCat cell proliferation was decreased by Arthrobacter sp., Bacillus sp., Brachybacterium sp., Brevundimonas sp., Kocuria sp., Mammaliicoccus sp., Norcardia sp., Prestia sp., Phychrobacillus sp., and Rhodococcus sp., while it was affected by the other bacteria. Therefore, these results have suggested that the airborne floating bacteria could be considered as the marker for the environmental risk management against atopic dermatitis, and it is needed for controlling the bacteria number that suppressed the proliferation of HaCat cells.

식중독 세균에 의한 biofilm 형성을 억제하는 효과를 시 험하기 위하여 cinnamon, clove 및 lemongrass 정유의 휘 발성 성분을 분석하였다. 또한 정유의 주요 항균활성 성 분이 polyethylene과 stainless steel 표면에서 식중독 세균 에 의한 biofilm 형성을 억제하는 효과에 대하여 조사하였 다. Cinnamon 정유의 주요 휘발성 성분은 cinnamaldehyde (38.30%), linalool (9.61%), β–caryophyllene (8.90%) 및 1,3,4-eugenol (8.19%)로 동정되었다. Clove 정유의 주요 휘발성 성분은 1,3,4-eugenol (61.84%)로 분석되었다. Lemongrass의 주요 휘발성 성분은 citral의 이성질체인 geranial (19.11%)과 neral (19.23%)로 검출되었으며, citral 은 isomeric acyclic monoterpene aldehydes로서 geranial (trans-citral, 19.11%)과 neral (cis-citral, 19.23%)의 혼합물 로 분석되었다. Cinnamon, clove 및 lemongrass의 주요 성 분 중 cinnamaldehyde, linalool, eugenol 및 citral이 disc diffusion assay에 의해 시험한 6종의 식중독 세균에 대하 여 강한 항균활성을 나타냈다. Eugenol (0.1%)과 citral은 polyethylene 및 stainless steel coupon 표면에서 식중독 세 균에 의해 형성된 biofilm에 대하여 강한 억제 작용을 나 타났다. Cinnamaldehyde (0.1%)는 Listeria monocytogenes ATCC 19112와 Staphylococcus aureus KCCM 11812에 의 해 형성된 biofilm에 대해 장한 억제 작용을 나타냈다. 연 구 결과 cinnamaldehyde, eugenol 및 citral 처리에 의해 식 중독 세균에 의한 biofilm 형성을 억제가 가능할 것으로 판단된다.

To isolate aerobically and identify the diversity of halophilic bacteria in the soil around two ports, Daepopogu and Hwasun Port, on Jeju island, a total 46 halophilic bacteria strains were isolated and phylogenetically analysed. The isolated strains were divided into 3 phyla, 8 families, 16 genera and 23 species. The main taxa was the Bacilli class, which included 50.0% of the strains with 3 families, 10 genera and 15 species of Bacillaceae, Exiguobacterium_f and Planococcaceae. The second taxa was the Gammaproteobacteria class, which included 45.7% of the strains with 4 families, 5 genera and 7 species of Aeromonadaceae, Halomonadaceae, Marinobacteraceae and Vibrionaceae. The isolated strains were tested for hydrolytic enzymes, amylase, lipase and protease activity, and 31 strains showed activity of at least one enzyme. Furthermore, auxin activity was determined in 7 strains. This study showed that the isolated strains have possible applications in the food and agricultural industries and have importance as a genetic resource in Korea.

양파 종자는 양파 생산을 위한 기초적 투입자원이기 때문에 건전한 종자는 양파 생산에서 매우 중요하다. 양파 종자에서 분리한 박테리아 중에서 양파 종자 발아를 억제하는 균주 OS1을 분리하였다. 분자 동정을 위해 16S rRNA 유전자 영역의 DNA를 분석하였고 이를 바탕으로 한 유연관계 분석을 통해 OS1 균주를 Leclercia adecarboxylata로 동정하였다. L. adecarboxylata는 면역 저하 환자 또는 기저 질환이 있는 환자에게 기회감염을 일으키는 것으로 알려져 있다. L. adecarboxylata OS1은 휘발 물질을 생산하고 이 휘발 물질이 양파 종자 발아를 억제하는 것으로 분석하였다. 특히, 양파 구근을 이용한 병원성 검정에서도 L. adecarboxylata OS1는 갈변증상과 썩음증상을 유발하였다. 아직 L. adecarboxylata에 의한 대규모 포장 또는 수확후병으로 인한 양파 피해사례가 보고된 적은 없지만, 이러한 결과는 L. adecarboxylata는 잠재적인 양파 병원체가 될 수 있음을 시사하고 양파 생산에 잠재적 위협 요소가 될 가능성을 보여준다. 또한, L. adecarboxylata는 인체 기회감염 병원체이기 때문에 양파로부터 언제든지 인체로 감염될 수 있는 위험성을 갖고 있다고 볼 수 있다.

A field study was conducted to reduce airborne bacteria by supplying active ions to indoor spaces used by vulnerable human groups spending substantial amounts of time in places such as schools and hospitals. In an experiment conducted during school hours (8:00-15:00), the average number of airborne bacteria in classrooms was 345.53 CFU/m3 or more without active ions. However, ion supply reduced the airborne bacteria to an average of 113.23 CFU/m3, indicating an efficiency of 61.61%. As a result of tests in 33 rooms used for surgery in small and middle sized hospitals, ion supply for 2 to 4 hours reduced the average airborne bacteria concentration from 243.88 CFU/m3 to 104.34 CFU/m3, representing a 41.53% reduction. A laboratory test to confirm the ion activity has shown that the mortality rate of E. coli used as a test bacterium increased with exposure time to ions. The initial colony number of E. coli was 251 CFU, but decreased to 4 CFU after 60 minutes of exposure to active ions. Therefore, it was confirmed that the supply of active ions can contribute to the control of airborne bacteria in the indoor environment of schools, hospitals and other public facilities.

Airborne bacteria are expected to float in the mushroom cultivation house, as it is a special environment with high humidity and high temperatures. Their concentration and diversity in the indoor air of the cultivation house could effect the health of farmers and the quality of mushrooms. To examine whether microbiota of airborne bacteria change from year to year, we measured the indoor temperature, humidity, and airborne bacterial concentration from mushroom cultivation houses located in six regions in Korea from 2020 to 2021, and isolated and identified airborne bacteria. The surveyed data were compared and the bacterial diversity of the 1st year and the 2nd year were determined. Based on the average temperature and humidity data surveyed, it can be seen that the temperature and humidity environment in the cultivation houses is such that bacteria can easily reproduce. It was observed that the temperature inside the cultivation houses tends to be higher or lower depending on the season and correlates with the temperature outside the cultivation houses. In the first year survey, 32 species of 20 genera were identified, and in the second year survey, bacteria belonging to 29 species of 22 genera were identified. Among them, the most detected species were all species belonging to the genus Bacillus. There were only three species (Bacillus altitudinis, Brevibacterium frigoritolerans, and Staphylococcus epidermidis) that were continuously isolated in common. Our results showed that the species of floating bacteria greatly vary from year to year even for the same cultivation houses.

본 연구에서는 토지피복유형에 따른 바이오에어로졸 중 배양성 세균분포를 파악하기 위하여 충청북도 충주시에 위치한 3가지 유형을 대상으로 실시하였다. 토지피복에 따른 기상요소와 바이오에어로졸을 조사하여 측정지점별 미기상변화에 따른 세균 분포를 비교·분석할 수 있었다. 측정지점별 미기상 중 기온의 차이는 도시 숲 8.7℃, 수변 녹지 10.8℃, 도심지 10.2℃로 측정지점 중 도시 숲이 가장 낮은 기온 값을 보였으며, 상대습도는 도시 숲 61.8%, 수변 녹지 59.3%, 도심지 55.7%로 도시 숲이 가장 높은 것으로 분석되었다. 동정된 세균은 43속 99종으로 나타났다. 배양된 세균의 종 다양성 측면에서는 수변녹지 22속, 도시 숲 21속, 도심지 17속으로 조사되었으며, 종수의 경우 수변녹지 37종, 도심지 31종, 도시 숲 31종으로 나타났다. 3가지 유형의 측정지에 모두 존재하는 종은 Bacillus toyonensis와 Pseudarthrobacter oxydan으로 나타났으며, 또한 도시 숲과 수변녹지와 같은 녹지지역에서 존재하는 Herbiconiux flava종을 확인하였다. 측정지점별 미기상환경에 따른 세균농도의 변화를 분석한 결과 도시 숲 333CFU/㎥, 수변 녹지 287CFU/㎥, 도심지 173CFU/㎥ 순으로 나타났으며, 미기상 중 기온변화에 따른 측정지점별 농도변화는 미비하였다. 그러나 상대습도와 풍속은 농도변화와 유사한 경향을 보인 것으로 분석되었다. 따라서 본 연구에서는 토지피복유형에 따른 바이오에어로졸 중 배양성 세균의 분포 정도를 파악하여 측정지점별 바이오에어로졸의 특성을 비교·분석하여 추후 건강한 도시 관리 및 녹지조성의 기초자료를 제공하고자 하였다.