아미노글리코사이드계 항생제(Aminoglycosides, AGs) 는 그람음성균과 양성균에 광범위하게 작용하는 동물용 의약품으로, 최근 배양육에 사용된다고 알려져 있어, 안 전성 관리를 위한 분석법 마련이 반드시 필요하다. AGs 는 고극성 화합물로 성분 간의 분리를 위해 이온쌍 시 약(ion-pairing reagent, IPR)을 사용하고 있으나 IPR을 이동상에 첨가하는 기존 분석방법의 경우 용매가 흐르 는 동안 질량분석기로 주입되는 IPR로 인해 기기적인 문 제가 발생할 가능성이 높아, IPR를 바이알에 직접 첨가 하는 분석방법을 검토하였다. 본 연구에서 10종 AGs 성 분에 대한 분석방법을 확인하고 유효성을 검증하였다. 검출한계와 정량한계는 각각 0.0001-0.0038 mg/kg 와 0.004-0.011 mg/kg의 범위로 나타났으며, 0.01-0.5 mg/ kg 범위 내의 직선성(R2)은 0.99 이상이었다. AGs의 시 료 회수율을 확인하고자 소고기와 세포배양배지(cell culture medium) 매질에서 회수율과 상대표준편차로 나 타낸 정밀도를 확인한 결과 각각 70.7-120.6% 및 0.2 to 24.7%로 나타났다. 기존의 이동상에 IP 첨가 방법과 비 교하였을 때 유사한 수준으로 양호하였다. 검증된 AGs 분석법은 국내 유통되는 닭고기, 소고기, 돼지고기 15품 목과 배양육 배지 첨가제 6품목에 적용해보았다. 그 결 과 국내 유통되는 육류 15품목 모두 AGs 성분이 검출 되지 않았으나, 세포배양배지에서 streptomycin은 695.85- 1152.71 mg/kg, dehydrostreptomyci은 6.35-11.11 mg/kg 로 검출되었다. 따라서 IRR을 바이알에 직접 첨가하는 LC-MS/MS 방식은 육류, 세포배양배지, 배지첨가제 중 AGs 분석 및 안전성 평가를 위한 기초자료로 활용될 것 으로 기대된다.

소고기의 건식 숙성 기간을 단축하고자 연육과 관련된 효소 활성이 높은 15-36oC 온도범위에서 저습도로 숙성할 수 있는 라디오파 숙성장치를 개발하였다. 이 장치는 평행 판 전극 사이에 소고기를 넣고 라디오파를 가하여 유전가열이 되는 현상으로 고기의 온도를 높였고, 냉풍을 이용하여 습도를 낮춰서 고기표면이 건조되게 하였다. 이 장치를 이용하여 2등급 소고기 채끝 부위를 숙성시킨 결과 40 W/ kg로 가열하여 고기 품온이 30oC를 초과하는 온도 범위 24 h 숙성할 경우 12.3%, 10-30oC 온도 범위에서 숙성시킬 경우 55.2% 연육효과가 나타났다. 라디오파 숙성 중 제상 과정 없이 냉풍을 계속 가할 경우 미생물 증식에 의한 문제가 발생하지 않았으며, 이러한 연육 효과는 기존 건식숙 성 21일간 절단강도 17% 감소하는 것에 비해 매우 단시간 나타난 것으로 숙성기간을 크게 단축할 수 있음을 확인 하였다. 닭고기와 돼지고기는 육질의 차이로 라디오파 숙성에 의한 연육 효과가 나타나지 않았다.

In this study, the content of water-soluble vitamins such as vitamin B1 (thiamin), B2 (riboflavin), B3 (niacin), B5 (pantothenic acid), B6 (pyridoxine), B7 (biotin), B12 (cyanocobalamin), and C (ascorbic acid) in dishes containing meat and seafood consumed in Korea were determined by high-performance liquid chromatography (HPLC) with ultraviolet and fluorescence detection. All analyses were performed under strict quality control of vitamins B1, B2, B3, B5, B6, B7, B12, and C. The highest content of vitamin B1 was observed in Bugeo-gangjeong (1.373 mg/100 g) and the highest level of vitamin B2 (5.162 mg/100 g) was found in pig liver. Bugeo-gangjeong showed the highest content of vitamin B3 (21.676 mg/100 g), and kkomak-muchim contained considerable amounts (43.310 mg/100 g) of vitamin B5. Vitamin B6 was not detected in most seafood dishes except for yangnyeom myeongran-jeot (0.274 mg/100 g) and was present at low levels or not present at all in meat dishes. The highest content of vitamin B7 was 6.506 μg/100 g in saeu-jeon and kkomak-muchim showed the highest content (21.132 μg/100 g) of vitamin B12. The highest content of vitamin C was in yangnyeom myeongran-jeot (84.508 mg/100 g). In addition, the analysis methods of each water-soluble vitamin were verified. These results showed that seafood-based ingredients in several dishes could be a good source of water-soluble vitamins.

This study examined the factors influencing the purchase intention of meat substitutes. A survey was conducted on 589 consumers in Korea (297 people) and China (292 people). The perception of meat substitute foods was lower in Korean consumers than in Chinese consumers, but there was no significant difference. The purchase intention of meat substitute foods was lower in Korean consumers than in Chinese consumers (p<0.01). Korean consumers’ perception of meat substitute foods was higher in males than in females (p<0.01). The purchase intention of meat substitute foods also showed the same trend (p<0.001). Chinese consumers’ perception of meat substitute foods was higher in males than in females. The overall purchase intention of meat substitute foods was significantly higher in males than in females (p<0.05). The perception of meat substitute foods by Korean consumers’ was higher in their 40s and 50s than in their 20s and 30s. The purchase intention of meat substitute foods was also high in their 40s and 50s (p<0.01). On the other hand, the perception of meat substitute foods by Chinese consumers’ was higher in their 20s and 30s than in their 40s and 50s (p<0.01). The purchase intention of meat substitute foods also showed the same trend (p<0.01). Korean consumers’ perception of meat substitute foods had a significant positive effect on the purchase intention of meat substitutes (p<0.001). Chinese consumers’ perception of meat substitute foods also had a significant positive effect on the purchase intention of meat substitute foods (p<0.001). Overall, food technology neophobia has a significant negative effect on the purchase intention of meat substitutes (p<0.05).

In this study, hardness, which is one of the most important texture properties of meat products, was investigated in order to evaluate the potential of using crushed meat products as food for seniors. The hardness of crushed meat products in the Korean market, such as hamburg steak (12 products), meatball (8 products), ddeokgalbi (rib patties, 13 products), and wanja (meat dumplings, 10 products) were analyzed through a texture analyzer. Also, the hardness of UDF 1st grade hamburg steak (Japanese care food) was assessed in comparison with the texture characteristics of crushed meat products. The mean value of hardness of 43 crushed meat products purchased in the Korean market was 64,733±12,319 N/m2 (23,650-102,780 N/m2) while the hardness of UDF 1st grade product was 50,910±4,990 N/m2. The hardness of 16 crushed meat products was not significantly different from that of UDF 1st grade products (p<0.05). On the other hand, crushed meat products had higher relative standard deviation (RSD, %) values of hardness (mean: 19.6%, from 7.8 to 43.1%) than the RSD of UDF 1st grade products (9.8%) except for three products. Therefore, crushed meat products could be a developable “food for seniors” if deviations of texture are minimized. These findings on texture properties could be an effective data base for the development and quality control of “food for seniors” using meat materials.

본 연구에서는 한국에 거주하고 있는 파키스탄인을 중심으로 각각 다른 유형의 육류에 대한 파키스탄인 소비자의 행동, 인식 및 선호도를 분석하였다. 본 분석을 위한 자료는 한국에 거주하는 파키스탄인을 대상으로 한 설문조사를 통하여 수집되었다. 본 연구에서는 설문에 응한 응답자 중 423 명의 설문지를 토대로 빈도분석과 다항로짓회귀분석을 실시하였다. 분석 결과 닭고기가 가장 선호하는 고기 (38.3 %)로 나타났으며, 양고기 (25.5%), 어류 (24.3%), 쇠고기 (7.3%) 순으로 선호하는 것으로 나타났다. 설문조사 결과 응답자 중 남성이 62.9%, 연령대는 30-40세가 51.4%로 높게 나타났다. 분석결과에 의하면, 나이, 신앙수준, 제품포장, 영양가, 감각적 특성 (맛, 냄새, 냄새, 색) 그리고 할랄 인증 유무가 각각의 육류 유형에 따른 소비자의 만족도에 중요하게 영향을 미치는 것으로 나타났다.

바다속 60 km 깊이의 수압과 같은 압력을 사용하여 순간적이고 균일한 압력전달로 가열공정을 최소화함으로써 신선한 맛과 향, 텍스처, 비타민의 유지 등 품질보존을 할 수 있는 기술인 HPP는 식중독과 부패미생물을 억제하는 효과를 가져올 수 있는 기술로 현재 가장 활발히 연구되고 있는 분야이다. 기존의 열처리가 단백질의 변성, 화학적 변화, 전분의 호화, 효소의 활성에 영향을 미쳐서 보존성을 유지한다면 HPP는 열처리의 장점은 그대로 갖고 있으면서 열처리에서 야기되는 원치 않는 화학적 변화를 최소화할 수 있다는 장점을 갖고 있다 보존기간이 길어 소비자들이 우려하는 첨가물의 사용이 필수적인 육류가공품에 대해서는 초고압처리에 대한 국내 연구가 미비하여 본 연구에서는 대표적인 육가공품인 슬라이스햄에 대해 600MPa 초고압처리(0분, 4분, 6분)가 이들의 신선도에 미치는 영향을 실험하였다. 수분함량은 48~69%, 염도 1.07-1.11%에서 변화를 보이고 있었고, pH는 처음 6.4-6.5에서 6.1-6.15까지 낮아졌으나 대조군과 처리군간의 차이는 나타나지 않았다. 초고압처리 후 20oC에서 보관한 일반세균 결과는 4주까지 모든 군에서 미미한 수준에서 일반미생물이 발견되었으나 5주부터는 대조군과 HPP 6분 처리군에서 105을 초과하였고 7주에는 4분 처리군, 6분처리군에서 식품으로 섭취가 불가능한 106을 초과하는 것으로 나타났다. 대장균군은 20oC 가중실험으로 7주간 관찰하였음에도 불구하고 모든 군에서 대장균군이 발견되지 않았다. 육류의 단백질 신선도 판정에 사용되는 방법인 VBN은 4주차까지는 1 mg% 미만의 VBN값을 보였고 5주부터는 1~2 mg%의 값을 나타냈다. 그러나 군별 차이는 나타나지 않았다(p > 0.05). 육의 조직내 지방 산패 정도의 지표로 사용되는 TBA는 7주가 끝날 때까지 0.18 mgMA/kg 이하였고 이 수치는 신선육의 범위에 들며, 처리군별 차이를 보이지 않았다. 본 실험에서는 포장재의 변성이 발생하지 않았고 가스의 발생으로 인한 부풀어 오름도 발생하지 않았는데, HPP의 효과가 전혀 관찰되지 않은 사실은 포장재의 밀착으로 인한 공기와의 차단만으로 기본적인 보존 효과를 보인 것이 아닌가 사료된다.

칠면조는 슈퍼 푸드 중 유일한 육류 식품으로 선정되어 칠면조의 육류 소비는 더욱 증대될 것으로 기대된다. 이 에 따라 식품 중 사용된 동물성 원료인 칠면조의 신속하고 정확한 판별법 개발이 요구된다. 본 연구에서는 칠면조 를 정확하고 민감하게 검출하기 위해 TaqMan® probe를 활용한 real-time PCR 방법을 개발하였다. 종 특이 primer와 probe는 미토콘드리아의 cytochrome b 유전자를 대상으로 개발하였고, 특이성은 총 19 종의 동물성 원료를 대상으 로 확인하였다. 개발된 검출법의 검출한계는 칠면조 DNA를 10 ng 으로 부터 10배씩 희석하여 확인한 결과 0.1 pg 이었고, 동물성 식품원료인 소와 닭에 칠면조를 50, 25, 5, 1, 0.1% 비율로 혼합하여 확인한 결과 0.1 %까지 검출되 었다.

Recently, the incidence of cardiovascular diseases including high blood pressure and heart disease has increased with increased meat consumption in Korea. This study was performed to determine the optimal mixing ratio among ratios of 0, 1:0, 0:1, 1:1, 2:1, and 1:2 of mulberry leaf powder to mulberry fruit powder based on the anti-inflammatory effects. Then, the quality characteristics of the sauce for meat prepared with different mixing ratios (0, 1:1, 1:2, 1:3, 1:4, and 1:5) of mulberry leaf powder to mulberry fruit powder were assessed. The inhibitory effects of ML2MF1 (2:1 ratio of mulberry leaf powder to mulberry fruit powder) on NO and TNF-α production were superior to those of other mixing ratios. With respect to color values of the sauce for meat prepared with different mixing ratio of mulberry leaf powder to mulberry fruit powder, as the mulberry fruit powder mixing ratios was increased, redness was increased but lightness and yellowness were decreased. The sensory evaluation of ML1MF5S (1:5 ratio of mulberry leaf powder to mulberry fruit powder) sauce for meat showed the highest score with respect to color, smell, taste, harmony with meat and the overall preference. In conclusion, as the mulberry leaf powder mixing ratio was increased, the anti-inflammatory activities were increased. But sensory evaluation scores were increased as the mulberry fruit powder mixing ratio was increased. Therefore, by performing further study, the method to increase mulberry leaf powder in the mixing ratio for improving the sensory evaluation should be provided.

본 연구에서는 포장방법 (진공, 일반)과 저장온도(4℃, -20℃)를 달리한 육류 (소고기, 닭고기) 및 생선에 대해 저선량 감마선 반복조사를 적용하였을 때 나타나는 병원성미생물 E. coli O157:H7 및 S. Typhimurium의 사멸효과를 확인하였다. 소고기와 닭고기의 경우 E. coli O157: H7은 최소 2 kGy 이상의 단일조사와 0.5 kGy 이상으로 반복조사 시 생육이 확인되지 않았고, S. Typhimurium은 최소 2kGy 이상의 단일조사와 1 kGy 이상으로 반복조사 시 생육이 확인되지 않았다. 생선의 경우 최소 0.5 kGy 이상의 단일 및 반복조사 시 E. coli O157:H7 와 S. Typhimurium 의 생육이 확인되지 않았다. 육류보다 생선이 낮은 선량으로 미생물을 사멸시킬 수 있었으며, 전반적으로 포장 방법에 따른 조사선량의 차이는 없었으나 저장온도의 경우 냉장보다 냉동의 경우가 좀 더 높은 조사선량을 요구하는 것으로 확인되었다.

The objective of this study was to develop a simultaneous method of 8 penicillin antibiotics including amoxicillin, ampicillin, cloxacillin, dicloxacillin, nafcillin, oxacillin, penicillin G and penicillin V in meat using LC-MS/MS. The procedure involves solid phase extraction with HLB cartridge and subsequent analysis by LC-MS/ MS. To optimize MS analytical condition of 8 compounds, each parameter was established by multiple reaction monitoring in positive ion mode. The chromatographic separation was achieved on a C18 column with a mobile phase of 0.05% formic acid and 0.05% formic acid in acetonitrile at a flow rate of 0.2 mL/min for 20 min with a gradient elution. The developed method was validated for specificity, linearity, accuracy and precision in beef, pork and chicken. The recoveries were 71.0~106%, and relative standard deviations (RSD) were 4.0~11.2%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.003~0.008 mg/kg and 0.01~0.03 mg/kg, respectively, that are below maximum residue limit (MRL) of the penicillins. This study also performed survey of residual penicillin antibiotics for 193 samples of beef, pork and chicken collected from 9 cities in Korea. Penicillins were not found in all the samples except a sample of pork which contained cloxacillin (concentration of 0.08 mg/kg) below the MRL (0.3 mg/kg).

A simultaneous determination was developed for 9 aminoglycoside antibiotics (amikacin, apramycin, dihydrostreptomycin, gentamicin, hygromycin B, kanamycin, neomycin, spectinomycin, and streptomycin) in meat by liquid chromatography tandem mass spectrometry (LC-MS/MS). Each parameter was established by multiple reaction monitoring in positive ion mode. The developed method was validated for specificity, linearity, accuracy, and precision based on CODEX validation guideline. Linearity was over 0.98 with calibration curves of the mixed standards. Recovery of 9 aminoglycosides ranged on 60.5~114% for beef, 60.1~112% for pork and 63.8~131% for chicken. The limit of detection (LOD) and limit of quantification (LOQ) were 0.001~0.009 mg/kg and 0.006~ 0.03 mg/kg, respectively in livestock products including beef, pork and chicken. This study also performed survey of residual aminoglycoside antibiotics for 193 samples of beef, pork and chicken collected from 9 cities in Korea. Aminoglycosides were not found in any of the samples.

The purpose of this study was to investigate changes in Korean meat consumption as well as meat consumption trends. During the Japanese occupation period, the supply of meat was considerably insufficient. However, meat consumption mainly in large cities has gradually increased. Especially, 'Pyeongyang cow', a specialty of Pyeongyang, started being raised as edible beef cattle in 1933. During the chaotic period following liberation from Japan, the price of meat sharply increased. However, as the meat supply stabilized, the 'beef grade system' was introduced in 1967. Since then, beef has sold according to region. During the early economic growth period of the mid-1970s, meat consumption rapidly increased, and foreign beef was first imported in 1976. The preference for beef was somewhat attenuated due to the outbreak of mad cow disease and economic slowdown of the 1990s, resulting in an increase in the consumption of pork, a replacement meat. During the recent period of economic development, meat consumption has somewhat fallen and remained low. In late 2003, with the occurrence of mad cow disease in the US, the demand for pork, and especially pork fatback, has sharply increased.

Physicochemical, microbiological and sensory characteristics were determined during storage at 15, 25, and 35℃ for six months to predict shelf-life of four fried rice retort dishes. Thiobarbituric acid (TBA) values were increased significantly and pH and acid values did not show any significant differences. Microbiological contamination levels were estimated as safe. Sensory evaluation were tested over 4 points during storage period. It maintained the commercial value during the shelf-life. TBA value as an effective quality indicator was used to estimate shelf-life with Arrhenius equation. The estimated shelf-life were 1,408 days (46 months) for beef fried rice, 1,353 days (44 months) for Ham fried rice, 1,164 (38 months) days for chicken curry fried rice and 1,182 (39 months) days for bacon tuna fried rice. In conclusion, shelf-life of all four fried rice dishes was predicted as longer than three years (36months) at room temperature.

The objective of this study was to develop a stock that can be used to make a variety of dishes. For this, stock was prepared with beef, chicken, and pork meat as animal meats as well as anchovy, mushroom, and sea tangle as other ingredients. The qualities of six[only four though] kinds of stocks made with beef only (B), beef and chicken (BC), beef and pork (BP), and beef added with chicken and pork (BCP), and seven kinds of stocks made with anchovy (A), mushroom (M), sea tangle (S), anchovy and mushroom (AM), anchovy and sea tangle (AS), mushroom and sea tangle (MS), and anchovy added with mushroom and sea tangle (AMS) were investigated by sensory evaluation. The most preferred stock made with animal meat was mixed with the most preferred stock made with other ingredients at a 5% level to test the synergistic effects of palatability. As a result, BCP stock and AMS stock obtained the highest scores for overall preference. BCP stock within the range of 30%-65% along with the mixed stock containing AMS (35%-70%) showed synergistic effects for palatability.

The purpose of this study was to investigate the Jorim of meat and poultry in Korean modern cookbooks. Jorim is a traditional Korean method of braising meat and poultry with soy sauce or gochujang. This study evaluated jorim in 10 books published in Korea from the end of 1800 to 1987 and to develop cooking models of beef jorim, pork jorim, and chicken jorim. The main ingredients in the jorim were beef, pork, chicken and pheasant. Other ingredients in the jorim included pine nuts, beef, eggs, radishes, bean sprouts, onions, bamboo shoots and pepper. In Sieuijeanseo, which was published at the end of the nineteenth century, the method called for braising meat directly in soy sauce. However, other modern cookbooks suggested boiling the meat prior to braising. Despite jorim being a popular sub-dish in Korea, there has been a decrease in the varieties available.