Dendropanax morbifera Leveille (Araliaceae) is an endemic species growing in the south-western part of South Korea that has been used in folk medicine and health functional food. In this study, we investigated an extract of quercetin in Jeju D. morbifera by varying different parts (fruit, sprouts, leaves, sprigs, and branches), harvest times, and extraction solvents. In addition, we aimed to establish a simple and reliable HPLC/UV analytical method to determination of quercetin for the quality control and base line data of the Jeju D. morbifera extract as a health functional food ingredient. The analytical specificity was determined with retention time and photo diode array (PDA) spectrum by analyzing quercetin using HPLC and comparing the results to those of extracts. This analytical method for quercetin was validated for its limit of detection (LOD), limit of quantitation (LOQ), precision, and accuracy. A high linearity in the standard calibration curve was obtained, with a coefficient of determination (R2) of 0.9996. Also, the LOD and LOQ values were found to be 0.28 μg/mL and 0.85 μg/mL, respectively, and the recoveries of quantified compounds ranged from 97.91% to 104.10%. Furthermore, the relative standard deviation (RSD) values of data from the intra- and inter-day precision analyses were less than 1.36% and 3.65%, respectively. As a result, the highest quercetin content among the extracts of Jeju D. morbifera leaves was found to be 20.14 mg/g, which was extracted at harvest in May (cultivation period 10 years) with 60% EtOH. All in all, we believe that the results obtained would be helpful in the development of nutraceutics and natural medicines and for the quality control of D. morbifera.

Background : Currently sprout ginsengs are produced in extensive variations of cultivation times, methods and specifications that are different in each farm in Korea. Also, regardless the effective ingredient content of ginseng, overgrown sprout ginseng are distributed so that the quality standardization of sprout ginseng is urgently required. The sprout ginseng market is only a segment of the Korean ginseng market still. However, If development of processed products utilizing the functionalities is connected, the market may exponentially grow. Also, if the use of sprout ginseng is extended to productions of drinks, cosmetics, facial masks and others in the cosmetics and health functional food industries, the export competitiveness to the global market is expected to increase. Therefore, this study aims the quality standardization of sprout ginseng to secure the market competitiveness in Korea and overseas by examining the property change following the cultivation phases. Methods and Results : For the overground growing in green houses, the sprout ginsengs planted in early April and early June show to be better in growing than the ones planted in early August. For the underground growing, while no significance is found among the cultivation periods, the sprout ginsengs planted in early June are excellent in terms of the root length and the weight of overground portion. Also, according to the examination on the stem properties of 2-year grown sprout ginsengs in each cultivation time, a trend that, as the growing period is longer from 25 days to 35 days, 45 days and 95 days, the hardness, gumminess, chewingness and springiness of stem are higher. And, on the hardness, gumminess, chewingness and springiness of leaf, while no statistically significance is found in the growing periods from 25 days to 45 days, the 95-day sprout ginsengs show high figures referring that the leaves becomes tougher. Conclusion : In case of cultivating sprout ginsengs in a green house, cultivating in April - June is advantageous for the overground and underground growing. And, it is determined that cultivating sprout ginsengs for 35 - 45 days produces leaves and stems with the most excellent chewing textures felt by consumers when chewing.

This study investigates, the physicochemical characteristics of Sengmaksan (SM) prepared with Liriope platyphylla (LP) that had been roasted for different times (0, 30, 60, and 90 min, denoted as S-0, S-30, S-60, and S-90, respectively) The Hunter’s color values such as lightness (L), redness (a), and yellowness (b) were the highest in S-0, while the lowest was found in S-90. The amount of soluble solid and reducing sugar content of S-60 were higher than the others. None of the samples exhibit significant differences in, their pH and acidity. The total content of phenolic compounds increased with the LP roasting time, but the total flavonoid and total anthocyanin contents of the SM decreased at the same time. The total ginsenoside (Ro, Rb2, Re, Rf, Rg1, Rg2, Rg3, Rh1, and Rh2) content did not show significant differences. The DPPH and ABTS radical scavenging activities increased according to the concentration, as well as with the LP roasting time. The ferric reducing antioxidant power (FRAP) showed trends similar to the radical scavenging activity, but it was more sensitive to the LP roasting time. From these results, the active ingredient in S-60 was higher, and the antioxidant activities of SM increased along with the roasting time of LP.

본 연구는 2015년과 2016년에 ‘상주둥시’ 감의 수확시기 를 달리하여 수확 후 PE필름, 1-MCP 및 AVG를 처리가 저온저장동안 과실의 품질에 미치는 영향을 조사하였다. 2016년 과실 수확시기는 2015년에 비하여 10일 빠른 조기 수확을 실시하였다. 과실의 에틸렌 발생 정도는 조기수확 한 과실들에서 현저히 낮았고, 과실의 경도는 저온저장동안 1-MCP 처리구들이 다른 처리구의 과실에 비하여 높게 유지되었다. 과실의 감모율은 수확기를 달리한 모든 과실 들에서 PE 필름 처리 과실들에서 현저히 억제되었다. 성숙기에 수확한 과실은 저온저장 중 과실의 꽃받침 부위과 경와부의 과피색(L* 및 b*) 변화를 보면 무처리와 PE필름 처리구에 비하여 1-MCP 처리구들이 그 변화정도가 낮았다. 과실의 부패와 연화정도는 PE필름 처리 과실들에서 높게 나타났지만 AVG 처리구들에서는 부패과 발생이 없었다. 그리고 조기수확한 과일은 성숙기에 수확한 과실에 비하여 경도가 높게 유지되었고, 과피색의 변화가 낮은 경향을 보였고, 성숙기에 수확한 과실들은 당도, 과피의 적색 (Hunter a 값) 발현 및 호흡율이 높게 나타났다. 따라서 감 과실의 저장성 향상을 위해서는 조기수확이 중요한 요인으로 작용하고 또한 수확 후 1-MCP 처리가 과일의 경도를 높게 유지하고 과피색의 변화를 억제하였으며, 과실의 감 모율은 PE 필름 처리에 의해 현저하게 억제됨을 확인하였다.

본 연구는 일반인을 대상으로 시대성이 반영된 각종 잠재적 위험요소와 원자력(방사선)발전의 위험성 대 한 인식을 분석하였다. 설문 대상은 다양한 계층으로 하고 그 중에 총 293부를 분석하였다. 그 결과, 잠재 적 위험요소 중에서 화재에 대한 위험도 인식이 높게 나타났으며, 다음으로 방사선 테러와 핵(원자력)에너 지의 위험도를 다른 위험요소들에 비해 비교적 높게 인식하였다. 연령별, 학력별, 정치 이념 성향에 따른 분석에서 원자력발전의 필요성과 위험성, 안전성에 대해서 상반된 결과를 나타내었다. 정치이념의 성향에 따른 잠재적 위험요소와 원자력발전에 대한 인식은 보수적 이념집단에서 긍정적인 인식을, 진보적 집단에 서는 부정적인 인식을 하고 있는 것으로 나타났다. 즉, 정치 이념의 성향에 따라 원자력발전 인식 분석에서 통계적 차이를 보였다. 따라서 원자력(방사선)발전 정책 방향 설정과 방사선 이용 관련 산업에 있어서 전문 가 의견과 일반인의 다양한 의견을 반영해서 결정되어져야 할 것으로 판단되고, 일반인도 사실을 바탕으로 객관적이고 과학적인 근거에 입각해 각종 잠재적 위험과 원자력(방사선)에 대해 막연한 불안감을 가지지 말고 유연한 대처를 할 필요가 있다고 판단된다.

Background : Recently, there have been dynamic researches conducted on stevia as natural sweetener subtitute for sugar, However, Researches related to harvest period and parts of plant in stevia are few. Therefore, this study was conducted to select optimum harvest time and parts by measuring the natural sweeteners contents and analyzing antioxidant materials and activity according to harvest times, parts of plant. Methods and Results : Stevia was cultivated in plastic house, The leaves were harvested from April to October and the stem were only harvest in July and September. Stevia leaves and stems were extracted using high temperature and pressure extraction: Dried stevia leaves of 5g were added by 100ml of distilled water equivalent to 20 times of dry weight, and the mixture were extracted by autoclave at 121℃ for 15min. The contents of Rebaudioside A and Stevioside of stevia leaves harvested from April to October showed a tendency to increase gradually from July to October as the temperature increased, but the contents of rebaudioside A and stevioside decreased slightly in August due to excessively high temperature. The extraction yield of stevia leaves were highest in October and September, and there was no significant difference in the other period. In the stevia stems, the extraction yield was lower than that of stevia leaves in general regardless of harvest time. Total phenolic contents and flavonoid contents according to harvest time showed little difference among treatment. Conclution : stevia leaves were better than stevia stems regarding the use of rebaudioside A and stevioside as natural sweeteners. Also, it was confirmed that the stevia leaves of July, September and October, except for the high temperature period of August, had superior in quality and quantity.

본 논문의 주 목적은 ｢J. 알프레드 프루프록의 사랑 노래｣에 나타 난 근대성과 시간의 의제가 지닌 중요성을 탐구하는 것이다. 산업화 와 도시화의 결과로 인간소외 현상이 심화되는 후기 근대의 도시로 부터 탈주하고자 하는 프루프록은 대립적인 인간관계가 해소된 마법 화된 시공간을 꿈꾼다. ‘상상적인 동일시’를 통해 구성된 이 세계는 기본적으로 여성적인 영역으로, 방안의 여인들로 대표되는 이 공간 에서는 교감과 친밀성을 바탕으로 하는 인간관계가 유지되고 있다. 또한 프루프록은 동일한 것의 반복을 특징으로 하는 근대의 일상적 시간을 특이성과 우발성이 충만한 차이의 시간으로 대체하고자 한 다. 하지만, 프루프록을 표준화하고 사물화하는 근대성의 시선의 회 귀와 더불어 그는 인간의 시간이 중단된 신화적 세계에 감금되며, 이것은 사회적 존재로서의 프루프록의 상징적 죽음을 의미한다.

Background : This study was conducted to determine the impact of temperature elevated and the effect of transplanting times based on climate change scenario on growth of 2-year-old korean ginseng (Panax ginseng C. A. Meyer.) in temperature gradient chambers (TGC). Methods and Results : As a plant materials, ‘Yunpoong’ was cultivated in TGC at ambient temperature(Amb), Amb+2℃, Amb+4℃ and Amb+6℃ respectively. Ginseng was also transplanted on March 29, April 12 and 26 respectively. Investigation on characteristic of aerial parts were carried out on 28, 56, 84 and 112 days after transplanting and characteristic of roots were conducted on October 19. As transplanting time was faster and temperature was higher, the growth of aerial parts were increased. Compared with those of ginseng transplanted on March 29 with Amb, the root weight which tend to decrease depending on late transplanting time and high temperature decreased about 11.1%, 35.4% and 42.4% in Amb+ 2℃, Amb+4℃ and Amb+6℃ respectively. Ginseng transplanted on April 12 and 26 decreased about 20.9%, 33.9% respectively. Conclusion : Consequently, the more transplanting time extend, the more quantity increased in all temperature treatment. So, it is possible to increase in quantity to advance transplanting time although high temperature will be caused by the climate change.

The evolution of the electro-mechanical impedance (EMI) of piezoelectricity (PZT) sensor was investigated to determine the setting times of fiber reinforced cementitious materials in this study. Penetration resistance test was also conducted to validate the EMI sensing technique. As a result, the setting times of fiber reinforced cementitious materials can be effectively monitored through the EMI sensing technique using an embedded PZT sensor.

Background : This study examined the effect of harvesting time on the growth, yield characteristics, and major beneficial components in Salvia miltiorrhiza. Methods and Results : Although plant height, stem diameter and branch length were not affected by harvesting time, the number of stems was highest when harvested in mid October. There were no differences in root length and thickness, however, the rhizome was thicker when it was harvested at the end of October or early November than when it was harvested in early and mid October. The dried root weight also showed a similar pattern. However, there was a statistically significant increase to 408 ㎏ (16%) in the rhizome weight when in late October and a rise to 455 ㎏ (29%) when harvested in early November. Harvest time had little effect on the content of the major component of S. miltiorrhiza. For example, salvianolic acid content rose from 9.42 to 9.64% with later harvest times, and tanshinone ΠA content was tended to be slightly more increased in mid October which S. miltiorrhiza has 0.22% tanshinon ΠA than in early October. Conclusions : According to these results, the optimum harvest time for S. miltiorrhiza is early November when plant or major component yields are hightest. There were no significant harvest time effects on the major beneficial components.

There has been little research on the harvesting time-dependent changes in the antioxidant activities of new varieties of highland cultures of Gomchwi (Ligularia fischeri) crossed with Turcs (Ligularia fischeri (Ledeb.)) and Nakai (Ligularia fischeri var. spiciformis), namely Sammany (S), Gommany (G) and Damogy (D). This study was conducted to assess the effect of different harvesting times on nutritional and health-related properties such as total phenolic contents, flavonoids, DPPH (1,1-diphenyl-2-picrylhydrasyl) free radical scavenging activities and reducing power. From these harvests, extracts were prepared using methanol. Total phenolic content in Jul 14-G (Gommany harvested on July 14, 0.172 ㎎·GAE/㎖) was higher than that in other products harvested after the same period (S, 0.154; D, 0.141 ㎎·GAE/㎖). Flavonoid content was higher in Jul 3-G (0.114 ㎎·QE/㎖), compared to Jul 3-S (0.113 ㎎·QE/㎖) and Jul 14-D (0.089 ㎎·QE/㎖). Antioxidant activities were higher in samples harvested after June 12 in all cases. On July 14, the highest DPPH free radical scavenging activities among all harvest dates were seen (92.875~94.595%). The reducing power was also dependent on harvest day (Abs 0.5~0.6 on July 14), showing a pattern similar to that of DPPH free radical-scavenging activities. Antioxidant activity and harvesting times seem to correlate with total polyphenol and flavonoid contents.

경남 남해군에 자생하는 고유품종으로 품종보호 등록된 섬애약쑥의 채취시기와 건조방법에 따른 이화학적 특성과 항산화활성을 평가하였다. 섬애약쑥은 5, 6, 7월에 동일한 재배지에서 수확한 후 각각을 12일 동안 음건(SD) 및 60℃에서 7일간 숙성한 다음 90℃에서 220분간 저장하여(AD) 건조하였다. 전처리방법을 달리하여 건조한 섬애약쑥의 유리당을 분석한 결과 glucose만이 검출되었으며 함량은 0.42±0.02~0.43±0.01 g/100 g과 0.41±0.02~0.47±0.04 g/100 g이었다. 총 페놀화합물의 함량은 AD(1.29±0.08~2.90±0.08 g/100 g)와 비교하여 SD(1.85±0.09~3.45±0.14 g/100 g)에서 더 높았다. 물 추출물을 31.5, 62.5, 125, 250, 500 μg/mL의 농도로 제조하여 항산화활성을 평가한 결과 DPPH와 ABTS 라디칼 소거활성은 SD 5월 시료와 AD 7월 섬애약쑥 추출물의 활성이 가장 높았으며, FRAP도 이들 시료에서 여타 시료에 비해 유의적으로 활성이 높았다. β-Carotene에 대한 탈색 저해활성도 SD의 5월과 AD의 7월 시료에서 각각 25.53±2.85~81.43±2.56%, 35.98±2.22~79.00±1.42%로 가장 활성이 높았다. 이상의 결과 섬애약쑥은 5월 채취하여 음건하였을 때와 7월에 채취하여 숙성 가공하였을 때 유용성분의 섭취가 더 용이하며, 높은 항산화활성을 나타내어 기능성 식품 소재로 활용도가 높을 것으로 생각된다.

In this study we followed biofilm formation and development in a granular activated carbon (GAC) filter on pilot-scale during the 12 months of operation. GAC particles and water samples were sampled from four different depths (-5, -25, -50 and –90 cm from surface of GAC bed) and attached biomass were measured with adenosine tri-phosphate (ATP) analysis and heterotrophic plate count (HPC) method. The attached biomass accumulated rapidly on the GAC particles of top layer throughout all levels in the filter during the 160 days (BV 23,000) of operation and maintained a steady-state afterward. During steady-state, biomass (ATP and HPC) concentrations of top layer in the BAC filer were 2.1 μg·ATP/g·GAC and 3.3×108 cells/g·GAC, and 85%, 83% and 99% of the influent total biodegradable dissolved organic carbon (BDOCtotal), BDOCslow and BDOCrapid were removed, respectively. During steady-state process, biomass (ATP and HPC) concentrations of middle layer (-50 cm) and bottom layer (-90 cm) in the BAC filter were increased consistently. Biofilm development (growth rate) proceed highest rate in the top layer of filter (μATP = 0.73 day-1; μHPC = 1,74 day-1) and 78%∼87% slower in the bottom layer (μATP = 0.14 day-1; μHPC = 0.34 day-1). This study shows that the combination of different analytical methods allows detailed quantification of the microbiological activity in drinking water biofilter.

본고에서는 이용도의 시대인식과 조선적기독교를 연구하였다. 먼저 그의 시대인식을 파악하기 위해 그가 조선교회, 선교사 및 서구교회, 그리고 일본을 어떻게 인식하였는지를 고찰하였다. 또 이런 시대인식이 예수교회의 설립과는 어떻게 연결되는지를 살펴봄으로써 그의 조선적 기독교 운동의 특징이 무엇인지를 고찰하였다. 이용도는 조선교회의 정통주의적 주류 신앙에 만족하지 않았으며, 선교사 및 서구교회에 대해서도 비판적이었다. 그러나 그를 전형적인 반교회적, 반선교사적인 인물로는 평가할 수 없다. 그는 협성신학교를 입학한 후 정치적 민족주의를 포기하였다. 때문에 예수교회의 설립은 일본에 대한 민족주의적 동기가 내포되어 있었던 것은 아니다. 결론적으로 이용도가 예수교회를 설립한 이유는 조선교회의 신앙적 체질을 바꾸고자 한 그의 시도가 장로교회의 견해와 상충되었기 때문이다. 조선적 기독교 운동의 관점에서 예수교회는 서구교회로부터의 신학적, 경제적 독립보다는 신앙의 본질에 대한 견해 차이에서 발생하였다. 체험적이고 실천적인 신앙을 추구하였던 이용도 지지자들은 기성교회의 권위에 부딪히자 독자적인 교회의 설립으로 나아갔던 것이다.

Gastrodia elata Blume is a achlorophyllous orchid plant that has been used in traditional medicine to treat many human illnesses, such as vertigo, hemiplegia, and epilepsy. It contains three major pharmacological substances; gastrodin, ergothioneine, and vanillyl alcohol. Here we investigated the quantitative changes in those functional components by cultivation conditions and harvest times. The content of ergothioneine was different with cultivation areas, while gastrodin accumulated the higher levels than vanillyl alcohol regardless of cultivation areas. The smaller tubers contained the higher concentrations of gastrodin and the lower concentrations of ergothioneine than did the larger tubers. The cultivation under sun shade screen increased the concentrations of both gastrodin and vanillyl alcohol but decreased the levels of ergothioneine. We also determined the contents of functional substances depending on the harvest times. The concentration of gastrodin was higher in October than in April, while the level of ergothioneine was higher in April than in October. Interestingly, the use of sexually propagated seed tubers produced 1.9 times higher levels of gastrodin compared to vegetatively propagated seed tubers. In this study, we found that three major functional substances in G. elata tubers altered depending on various cultivation practices, which might be important to increase the economic values of G. elata tubers.