Bovine rotavirus (BRV) is one of the common gastrointestinal diseases that can occur in calves, causing severe diarrhea. This study was conducted to investigate the effects of oral administration of single-domain antibodies, single variable domain of heavy chain of HCAb (VHH), on the prevention and treatment of rotavirus-induced diarrhea in calves. Thirty calves were divided into five experimental groups (negative control, positive control, group A, group B and group C). Except for the negative control group, the experimental groups were inoculated with BRV on the 4th day after birth. The VHH supplementation groups were fed with a substitute milk containing different concentrations of VHH (1%, 2.5% and 7.5%). Changes in body weight, clinical symptoms, serum antibodies, and virus detection in feces were observed for a total of 10 days in each experimental group. The results showed a higher survival rate in the VHH supplementation groups compared to the positive control group. Clinical symptoms caused by rotavirus were alleviated in the VHH supplementation groups. The detection rate of rotavirus in feces also decreased. Gross findings indicated that the severity of disease in the VHH supplementation groups was milder than that in the positive control group. Therefore, VHH could be proposed as a useful alternative for the prevention and treatment of rotavirus-induced diarrhea in calves.

Obesity, a global health concern characterized by excessive fat accumulation, necessitates the discovery of anti-obesity compounds. Rottlerin, known for its anti-cancer effects as a mitochondrial uncoupler, has been a subject of interest. However, its impact on reducing intracellular lipid accumulation remains a gap in our understanding. This study aimed to fill this gap by dissecting the mechanism of rottlerin in 3T3-L1 adipocytes. We treated differentiated 3T3-L1 cells with 0-20 mM of rottlerin for 48 hours to assess its capability to induce lipid accumulation. Notably, we observed no cytotoxicity associated with the treatment of rottlerin up to 20 mM, indicating its safety at these concentrations. Lipid accumulation, measured by oil Red O, was downregulated dose-independently by rottlerin. We also found that key lipogenic enzymes, including SCD1 and DGAT1, were decreased. The transcription factor of lipogenic genes, SREBP1, was reduced by approximately 80% with rottlerin. LRP6, a crucial link between de novo lipogenesis mechanism reactions and Wnt signaling, was also degraded by around 70%. Interestingly, the downstream regulation of LRP6, b-catenin, and TCFL2 was diminished by rottlerin. Our data indicate that rottlerin alleviates adipocyte lipid accumulation by suppressing the LRP6/β-catenin/SREBP1c pathway. These findings underscore the potential of rottlerin as a safe nutraceutical for combating obesity.

The objective of this study was to investigate the anticancer effects of EMPS (edible mushroom mycelium polysaccharide: Tremella fuciformis) in animal models with colorectal cancer induced by AOM/DSS. The experimental groups consisted of Nor (normal), NC (AOM/DSS), EMPS (EMPS 50, EMPS 100), and PC (Fluorouracil). The NC group had the highest number of colon tumors, whereas it was observed that tumor occurrence was significantly reduced in the EMPS consumption group. The expression of Bcl-2, an apoptosis inhibitor, was significantly lower in the EMPS 50 & 100 and PC groups. On the other hand, the mRNA gene expression of Bax, a factor that induces apoptosis, was significantly higher in the EMPS 50 & 100 and PC groups compared to the NC group. The mRNA expression levels of TNF-α and COX-2 significantly increased in the NC group, but showed a significant decrease in the EMPS and PC groups, indicating inhibition of the cancer-promoting response of cells. At the phylum level of the mice's intestinal microbial composition, the proportion of Bacteroidetes tended to decrease, while the proportion of Firmicutes tended to increase with EMPS administration. This suggests that changes in the gut microbiota caused by inflammation can be influenced by dietary intake.

Obesity, characterized by excessive fat accumulation, poses global health risks, including metabolic disorders like type 2 diabetes and cardiovascular diseases, with its prevalence steadily rising. This study proposes that rottlerin induces anti-obesity effects by enhancing non-shivering thermogenesis in beige adipocytes D16 via LRP6 inhibition. As a result, treatment of D16 cells with rottlerin up to 5 mM showed no cytotoxicity. Rottlerin significantly increased the expression of proteins involved in substrate oxidation, such as UCP1 and PGC1a, while decreasing the expression of C/EBPb associated with adipogenesis. Additionally, PRDM16, regulating brown adipocyte development, exhibited increased expression. The phosphorylation of LRP6, an indicator for Wnt signaling and nutrient-sensing pathway, is decreased by rottlerin. In conclusion, the study highlights the reduced phosphorylation of LRP6 as a pivotal mechanism by which rottlerin promotes the “beigeing” of D16 adipocytes, subsequently inducing non-shivering thermogenesis. This underscores rottlerin’s potential as a natural bioactive compound with anti-obesity effects.

In this study, the expression of genes related to fruit ripening was investigated using realtime PCR. The study aimed to determine the effective conditions of calcium compounds (Calcium citrate, calcium chloride, calcium nitrate, GH-Ca, OS-Ca) and chitosan treatment to extend the shelf-life in the ‘Kumhong’ nectarine and ‘Madoka’ peach fruits. In this study, in the ‘Kumhong’ and ‘Madoka’ fruits, the expression of genes related to cell wall degradation, pectin lyase (PL), polygalacturonase (PG), and pectin methylesterase (PME), was inhibited by calcium, chitosan, or a mixture (Chitosan+CaCl2) treatment. In ‘Madoka’ peach, although the expression of the PG gene was suppressed at pre-harvest Ca and chitosan treatment, the expression of the PL gene was induced at pre- and post-harvest Ca and chitosan treatment compared to the control. The spray of calcium, chitosan itself, or a mixture (Chitosan+ CaCl2) on the trees at the ripening stage and harvested fruits can extend the shelf-life by suppressing the expression of cell wall degrading enzymes genes (PL, PG, PME) in ‘Kumhong’ nectarine and ‘Madoka’ peach tree. These results provide valuable information for the development of technology for the extension of the shelf-life of peach and nectarine fruits.

PURPOSES : Sprinkler facilities play a crucial role in extinguishing fires in the early stages of a building blaze. Data indicate that more than 96% of fires are suppressed by sprinklers before growing out of control. However, corrosion and pitting of the sprinkler piping can reduce system performance as facilities age. The purpose of this study is to develop an eco-friendly water-soluble corrosion inhibitor to improve the reliability and longevity of sprinkler piping. METHODS : This study compared and analyzed silicate-based sprinkler piping shrinkage agents as corrosion inhibitor against existing commercial options. Tests were conducted to evaluate reactivity with fire extinguishing water, including electrolyzed reduced water and normal water. The anticorrosive performance of the silicate-based corrosion inhibitor was evaluated under various conditions to establish suitability before potential development or commercialization. RESULTS : The new corrosion inhibitor demonstrated eco-friendly performance. In testing, none of the primary four hazardous substances that pose the most risk of harm to the human body (specifically, arsenic (As), lead (Pb), cadmium (Cd), and mercury (Hg)) were detected. The inhibitor effectively reduced corrosion of carbon steel (SPP), with weight loss rates averaging 0.12% and not exceeding 0.27%. For copper (CDA 110) weight loss rates were up to 0.03%. Testing under constant temperature and humidity conditions show that the inhibitor kept weight loss was below 0.002 g, with no significant numerical value for the weight loss rate. Overall, the results indicate the potential for an environmentally-safe corrosion inhibitor. CONCLUSIONS : The optimal silicate-based sprinkler system can provide real-time sensor information such as oxygen saturation, pH concentration, and total dissolved solids (TDS). These metrics are closely related to the aging process. By linking this system with an aging monitoring solution, maintenance costs and safety could be improved over the lifespan of the sprinkler system. The sensors and monitoring capabilities are expected to enhance maintenance efficiency and equipment reliability.

배경/목적: ROS는 악성종양의 성장 및 공격과 관련이 있다. UDCA는 담도암 세포에서 진행과 전이에 밀접한 EGFR-MAPK 신 호 경 로 와 EMT를 억 제 한 다 . 이 연 구 는 UDCA가 담도암세포에서 ROS 생성 및 그에 관련된 바이오마커에 어떠한 영향을 주는지 알아보기 위해 시행되었다. 방 법 : 인간 간외 담관암 세포주인 SNU-245세포를 배양하였다. 세포생존율은 MTT assays로, ROS는 세포 ROS assays kit로 측정하였다. Western blotting으로 다양한 표적 단백질의 발현 수준을 측정하였다. 특정 유전자의 억제를 위해 siRNA를 사용하였고, 특정 유전자의 과발현을 위해 shRNA를 사용하였다. 결과: UDCA는 담도암 세포에서 DCA에 의한 peroxide와 ROS가 생성되는 것을 억제하였으며, DCA로 발현이 증강된 STAT3, PRX2 및 SOD2를 억제하였고, IGF-1에 의해 발현이 증강된 NOX2 및 NOX4를 억제하였다. 또한, 담도암 세포에서 SiRNA를 이용한 STAT3 및 PRX2의 억제는 UDCA 처치와 상관없이 EGF에 의해 약화된 E-cadherin 발현을 복원하고 EGF에 의해 증가된 N-cadherin 발현을 억제하였는데, 이는 UDCA의 EMT 억제에 PRX2/STAT3가 상당한 역할을 하는 것을 의미한다. 덧붙여, UDCA는 담도암 세포에서 DCA에 의해 억제된 catalase의 발현을 복원하였다. 한편, ShRNA를 사용한 NOX4의 과발현의 유도는 UDCA의 항종양 효과를 상쇄하였다. 결론: UDCA는 담도암 세포에서 ROS 생성을 억제하고, ROS 제거를 향상시킴으로써, 결국 EMT와 관련된 STAT3 및 PRX2를 억제한다, 따라서, UDCA는 ROS 활성도 및 EMT의 억제를 통하여 담도암 세포의 성장 및 침습을 억제하는 데 기여한다.

헤스페리딘(Hesperidin, HD)은 다양한 식물체에 존재하는, 강한 항산화 기능을 가진 대표적인 flavonoid의 일종이다. 본 연구에서는 수용성 HD인 Hesperidin glucoside(HDG)가 가지는 세포손상 회복, 항염증 인자억제 및 melanin 생성억제 활성을 세포수준에서 비교하였다. HDG는 HD에 당전이 효소반응 으로 제조되었으며, HD에 비해 20,000배 이상 수용해도가 증가되었다. HaCaT 세포주에 대한 세포독성은 HDG가 HD에 비해 월등히 낮았다. HD와 HDG는 모두 자외선 조사된 HaCaT 세포에서 세포생존율 회 복효과를 나타내었다. 또한 HD와 HDG는 세포내 산화질소(NO), 종양괴사인자-α(TNF-α) 및 인터루킨 -6(IL-6)과 같은 염증 매개체 및 cytokine을 감소시켰으며, HD 보다는 HDG의 효과가 다소 우수하였다. Melanoma B16F10 세포주를 이용한 melanin 형성능과 tyrosinase 저해활성을 측정한 결과, HD와 HDG 모두 효과를 나타내었으며 HDG가 약간 우수한 결과를 보였다. 결론적으로, HD의 당전이체인 HDG는 HD에 비해 동등이상의 세포손상 회복, 염증성 매개체 및 cytokine 억제능과 melanin 형성억제능을 나타내 었으며, HDG의 높은 수용성과 낮은 세포독성 등의 특성은 다양한 분야에서의 용도를 확대시킬 수 있을 것으로 보인다.

본 연구는 여름 및 초가을(6-9월)에 한반도에서 관측된 레윈존데 사운딩을 분석하여 대류가용잠재에너지와 대류 억제도가 깊은 습윤 대류 및 강수 발생 예측에 유용성이 있는지를 확인해보았다. 레원존데 사운딩은 열역학적으로 깊은 습윤 대류가 발생할 가능성이 높은 고 대류가용잠재에너지 저 대류억제도 그룹과 대류 발생을 억제시킬 수 있는 저 대 류가용잠재에너지 고 대류억제도 그룹으로 분류하였다. 이후, 두 그룹의 12시간 누적 강수량, 12시간 평균 최저운고, 12시간 평균 중하층운량의 분포 차이가 유의미한지 여부를 통계적 가설검정을 통해 확인하였다. 그 결과, 무강수인 경 우 21:01-09:00 KST 시간대의 12시간 평균 최저운고를 제외하고 두 그룹은 통계적으로 유의미한 차이가 있음이 검증 되었다. 이 결과는 고 대류가용잠재에너지 저 대류억제도 그룹이 저 대류가용잠재에너지 고 대류억제도 그룹보다 깊은 습윤 대류 및 강수 발생에 더 유리함을 시사한다.

The fungus Alternaria alternata, responsible for causing brown to black spotting on numerous fruits and vegetables globally, was identified in 2022 as the causative pathogen of brown spot disease in potatoes in Korea. In pursuing potential inhibitors against A. alternata growth, we evaluated 15 mushroom culture filtrates: eight from Trametes spp. and seven from Polyporus spp., known for their antibacterial and anticancer properties. Antifungal activity was assessed by exposing each filtrate to A. alternata on a paper disc. Four filtrates displayed inhibitory action against the fungus, albeit with mild effects. Our findings highlight the potential of Trametes and Polyporus fungi as emerging antifungal candidates, offering promise in preventing potato brown spots.

To develop a functional probiotic that inhibits gingipain, a major virulence factor of Porphyromonas gingivlais (P. gingivalis), we screened over 30 probiotic strains for their ability to inhibit gingipian activity. We investigated the inhibition of expression of gingipain genes kgp, rgpA, and rgpB as well as gingipain activity, using freeze dried cell-free supernatants of Weisiella cibaria SPM402 (WC402) and Lactobacillus paracasei SMP412 (LP412), both of which demonstrated antibacterial activity against P. gingivalis. Thus, it was verified that kgp expression was reduced by approximately 0.71±0.02 folds and rgpB expression was reduced by approximately 0.71±0.14 folds at a concentration of WC402 10 mg/mL. Meanwhile, at the same concentration of 10 mg/mL of LP412, kgp expression was reduced by approximately 0.19±0.08 folds, rgpA expression was reduced by approximately 0.09±0.02 folds, and rgpB expression was reduced by approximately 0.24±0.03 folds. At a concentration of 10 mg/mL, Kgp activity was inhibited by approximately 78.65±3.58% (cell associated gingipain, CAG), 82.45±1.22% (cell-free gingipain, CFG) by WC402 and 80.71±2.11% (CAG), and 85.81±0.05% (CFG) by LP412 respectively. Rgp activity was also effectively inhibited by approximately 78.6±1.01% (CAG), 86.78±0.47% (CFG) and 82.93±1.26% (CAG), 88.81±0.36% (CFG) by WC402 and LP412 respectively. Based on these results, W. cibaria SPM402 and L. paracasei SPM412 can be regarded as functional probiotics with the ability to inhibit gingipain activity and exhibit antibacterial effects against P. gingivalis.

The consumption of enoki mushrooms has been associated with cases of listeriosis produced by Listeria monocytogenes, highlighting the significance of sanitizing food-contact surface, such as the velcro used in welding processing of enoki mushrooms, to ensure microbial safety. We investigated the inhibitory activity of nine chemical disinfectants at regular concentrations against L. monocytogenes isolated from a mushroom farm environment. The bacterial suspension was prepared in phosphate buffered saline and mushroom extract broth and inoculated onto the velcro surface. After inoculation, most disinfectants reduced the initial 8 log CFU/coupon concentration by less than 2 log CFU/coupon during a 5-min treatment. Slightly acidic hypochlorous water showed a reduction of approximately 4 log CFU/coupon when tested for more than 30 min at the maximum allowable concentration of 200 mg/L. Sodium hypochlorite solution showed a reduction of approximately 5 log CFU/coupon when used at 100 mg/L for 60 min. Peracetic acid, at the maximum allowable concentration of 300 mg/L, showed the most effective reduction of 5 log CFU/coupon or more when the surface was treated with 37.5 mg/L for 30 min. These results indicate that peracetic acid can be used as the disinfectant strategy to control cross-contamination of L. monocytogenes on the velcro surface of plastic wrappers used in the welding processing of enoki mushrooms.

본 연구는 미국의 동아시아 안보전략과 해외주둔군 전략, 확장억제전 략의 변화를 분석하고, 북한의 핵무기 고도화에 대비한 주한미군과 핵우 산 정책을 고찰한다. 한미동맹이 체결된 1953년 이후, 미국은 한반도 안 보의 중심 역할을 수행했으나 비대칭 동맹으로 인한 동맹 딜레마와 미군 의 역할 및 규모 변화가 발생했다. 핵우산 정책은 냉전기 소련의 핵전술 에 대항하여 확장되어 현재까지 적용되고 있으며, 2023년에는 워싱턴 선 언을 통해 한미 핵협의그룹(NCG)이 창설되었다. 이를 통해 상시적인 확 장억제가 논의되고 있으며, 굳건한 한미 동맹체제와 안보공약이 SCM을 통해 재확인되었다. 이제는 냉전 시기의 미군 주둔과 자동개입 문제는 중국의 G2국가 발전, 북한의 핵과 미사일 도발 등으로 시대적 변수가 달 라져 있다. 따라서 미국의 주한미군 정책, 개입 문제, 확장억제전략에 대 한 신중한 검토와 대비가 필요하다. 본 연구는 미국의 동아시아 전략, 주 한미군, 확장억제전략에 대해 고찰하고 한반도 안보에 대한 전망을 목적 으로 한다.