Obesity occurs when the body consumes more energy than it requires and uses less energy, resulting in the accumulation of fat, which increases the number and size of fat cells in the body. It causes high blood pressure, type 2 diabetes, and joint crowding, making it difficult to fully treat. We investigated the effects of chito oligosaccharide(CHO) functional material, which suppresses fat accumulation, restores dyslipidemia values, and has no side effects.In this study, we investigated the ameliorative effects of CHO on obese mice fed a high-fat diet. The experimental groups were divided into 5 groups (n=10) of C57BL/6 mice: normal control (N), control group (C), and chito oligosaccharide low concentration (L), medium concentration (M), and high concentration (H) groups. We tested whether CHO has an effect on obesity through body weight and adipose tissue weighing, serum lipid testing, and histological examination. Experimental analysis showed that CHO reduced body weight and adipose tissue weight, improved the concentrations of TCHO, TG, HDL, and LDL, which are factors for dyslipidemia diagnosis, and decreased the diameter size of adipose tissue. These results suggest that CHO alleviates the levels of fat growth inhibition and dyslipidemia levels in obese-induced mice, and has a positive effect on obesity.

본 연구는 자일로올리고당의 과학적이고 체계적인 표준 화된 시험법을 마련하여 다양한 제형의 제품에 적용하고 자 하였다. 최적화된 시험법을 마련하기 위해 초음파 처 리 시간, 산 가수분해 시간 및 농도를 검토하여 전처리 방 법을 비교 평가하였으며, HPLC-UVD를 이용하여 시료 중 의 자일로올리고당을 분석하였다. 분석 시 1-phenyl-3- methyl-5-pyrazoline (PMP)으로 유도체화하고, photo diode array (PDA)가 장착된 high performance liquid chromatography (HPLC) (Nanospace SI-2)를 사용하였으며, 칼럼은 Cadenza C18 (4.6 × 250 mm, 3 μm)이었으며, 이동상은 pH를 6.0으 로 맞춘 20 mM 인산완충용액과 아세토니트릴을 78:22 비 율로 혼합하여 사용하였고, 0.5 mL/min 유속으로 254 nm 로 하여 분석하였다. 건강기능식품 등 시험법 마련 표준 절차에 관한 가이드라인에 따라 밸리데이션을 수행하고, 표준화된 시험법을 이용하여 유통 중인 건강기능식품 대 상 품목에 대해 시험법 적용 여부를 확인하였다. 표준화 된 시험법은 자일로올리고당을 함유한 건강기능식품 품질 관리에 대한 신뢰성을 더 높일 것으로 본다.

The global pet market is continuously growing as the number of single-person households increases along with the preponderance of the nuclear family, so pet-related industries are steadily growing. In addition, the market related to the health of companion animals is also increasing significantly. Regarding the health of companion animals, it is reported that the incidence of adult diseases, like humans, is growing mainly due to activities in indoor environments and the lack of exercise due to the lack of time for companion animals. This paper studies the antioxidant properties of Jerusalem Artichoke extract, which can be used as a raw material for functional foods related to adult diseases in companion animals by using substances extracted from Jerusalem Artichokes grown by the first author. Samples to be tested were extracted from hot water extraction and ethanol extraction methods according to conditions. To confirm the antioxidant properties, absorbance analysis, thin-layer chromatography (TLC) analysis, polyphenol measurement, flavonoid measurement, and radical scavenging ability measurement by DPPH were performed to determine whether the raw materials applied in this paper could be used. It was confirmed that Jerusalem Artichoke extract is a suitable additive raw material for functional pet food.

The endothelium, a continuous monolayer of cells that surrounds blood vessels, has a variety of physiological roles. Chronic exposure to psychological and physical stress, oxidative stress, and inflammation activates the endothelial signaling cascade, resulting in vascular dysfunction such as vasospasm, thrombosis, and abnormal vascular proliferation. Aging is also a significant factor in vascular dysfunction, mainly developing structural and functional changes in the endothelium. The molecules involved in endothelial dysfunction are typically angiopoietin-1 (Ang-1), Tie2, and tight junction proteins. Ang-1, an oligomeric-secreted glycoprotein, is required for the correct organization and maturation of newly-formed vessels. Ang-1 binding to tyrosine kinase receptors Tie-2 leads to the phosphorylation and activation of multiple signaling pathways related to vascular permeability. Endothelial junctions are another vital target of Tie-2 activation. Nutrition and food are closely connected with vascular dysfunction and permeability. The caloric restriction prevents age-related declines in endothelial function. Dietary patterns that prioritize moderate intake of fruits and vegetables, whole grains, low-fat dairy products, and lean meats improve endothelial function in the elderly. Natural products such as Centella asiatica, Pueraria montana, and Piper retrofractum have also been shown to help inhibit endothelial dysfunction. This review provides an update on aging-related vascular dysfunction and the role of food and nutrition.

This study was performed to investigate the quality characteristics and antioxidant activity of black soybean Sunsik product with functional food ingredients and functional labeling system. We prepared black soybean Sunsik (BS) containing black beans, cereals, and vegetables. Black soybean Sunsik with nondigestible maltodextrin and calcium lactate (BSN) was prepared by adding non-digestible maltodextrin and calcium lactate to the base recipe to apply a functional labeling system. The particle size in BS was 118.00 μm, whereas BSN was 127.00 μm. The respective L, a, and b color values of BS were 73.25, 2.36, and 14.21. The respective L, a, and b values of BSN were 73.21, 2.36, and 14.31. The respective water retention capacities of BS and BSN were 241.67% and 216.33%. No significant difference was observed between BS and BSN in the three physicochemical properties described above. However, the pH of BSN was 5.45, which was significantly lower than that of BS. The total respective phenolic contents of BS and BSN were 1.75 mg GAE/g and 1.61 mg GAE/g, and total respective flavonoid contents of BS and BSN were 6.36 mg RE/g and 5.95 mg RE/g. The antioxidant capacities of BS and BSN were compared via assays of DPPH and ABTS radical scavenging activities, FRAP, and reducing power. The antioxidant activities of BS and BSN increased in a dose-dependant manner. No significant difference between BS and BSN was observed in any measure of antioxidant capacity. These results suggested that the addition of functional food ingredients (non-digestible maltodextrin and calcium lactate) did not affect the quality characteristics and antioxidant activity of black soybean Sunsik.

본 연구는 건강기능식품 프랑스해안송껍질추출물 중 catechin, caffeic acid, ferulic acid, taxifolin에 대한 동시분석법을 개발하는 연구이다. 최적분석조건을 확립하기 위해 시료 채취량, 용매 조건을 비교 검토하였으며, UPLCMS/ MS를 이용하여 각 4개 성분에 대한 정확한 분석 및 분석시간의 효율성도 향상하였다. 분석 시 사용한 컬럼은 Acquity UPLC BEH C18이며, 정량이온으로 catechin, caffeic acid, ferulic acid, taxifolin 각각 133, 135, 245 및 248을 선정하였다. 확립된 시험법에 대해 특이성, 직선성, 검출한계, 정량한계, 정확성, 정밀성 등의 밸리데이션을 수행하였다. 4개 성분 모두 50-25000 mg/L 농도에서 결정계수(R2) 0.999이상으로 높은 직선성을 확인하였다. 또한 회수율은 84.9-104%이었고, 정밀성은 1.2-4.3%의 RSD를 확인하였다. 개발된 시험법은 프랑스해안송껍질추출물 중 catechin, caffeic acid, ferulic acid, taxifolin 분석을 위한 시험법으로 활용되기에 적합한 것으로 판단된다.

This study aim to evaluate dietary quality and nutritional status according to the consumption of health functional food using Nutrition Quotient for Korean elderly (NQ-E) for 288 elderly people attending senior welfare centers in Gyeonggi-do. The questionnaire consisted of items about general information, health functional food, and Nutrition Quotient for Korean elderly (NQ-E). Chi-squared test, Fisher's exact tests, and Analysis of Covariance (ANCOVA) were performed using the SAS program ver. 9.4. Among the male and female subjects, the female subjects consumed more health functional food. The results of the dietary quality and nutritional status difference according to the intake of health functional food showed significant differences only in the areas of variety and abstinence among nutrition quotient factors for men, while no significant differences were observed in any of the nutrition quotient factors for women. In conclusion, focused-nutrition education program and useful guideline is needed for promoting adequate consumption of health functional foods in elderly.

The objective of this study was to analyze labeling improvements and evaluate willingness to pay for health functional foods with a focus on the caution for intake. For this study, we conducted a survey on health functional food intake behavior, confirmation and improvements of cautions for intake. We assessed the willingness to pay for improvement of the caution for intake. Consumers anticipate improved immune function, and fatigue improvement after consumption of health functional foods. They mainly checked the function components related to efficacy and effectiveness, ingredients and their contents, ingestion amount and method, expiry date and best mode of storage, product name, and cautions associated with ingestion of health functional foods. They has been difficulties in obtaining sufficient caution information for intake from the current labeling method. Therefore, it is necessary to improve the labeling of caution for intake. The analysis indicated that about 5.14% of the respondents were willing to pay more if new labeling was introduces. However, there is still controversy over their safety, which is damaging to the consumers. Therefore, by providing consumers with accurate and detailed information on cautions for intake, it can contribute to securing safety and improving the quality of health functional foods.

본 연구는 건강기능식품의 복합제품 개발 및 일반식품 형태의 제품개발 확대에 따라 옥타코사놀의 특이성 및 감도 등이 개선된 분석법을 마련하여 향후 공정시험법에 활용할 수 있도록 하였다. 건강기능식품 중 옥타코사놀의 함량분석을 위해 GC-MS를 이용한 분석법을 확립하고 유통중인 제품을 대상으로 적용성을 검토하였다. 분석법 검증은 특이성, 직선성, 검출한계 및 정량한계, 정확성, 정밀성에 대해 수행하였다. 12.5~200.0 μg/L의 농도범위에서 r2 = 0.999 이상의 우수한 직선성을 확인할 수 있었으며, 검 출한계와 정량한계는 각각 4.5, 13.8 μg/L이었다. 공시료에 표준물질 첨가법을 이용하여 정확성을 검토하였으며 50, 100, 150 μg/L의 농도에서 각각 94, 93, 109%의 회수율을 확인할 수 있었다. 정밀성 결과에서는 시료 채취량을 80 mg, 120 mg, 220 mg으로 각기 달라하여 측정한 결과 상대표준편차는 1.8~2.4%로 확인되었다. 실험실간재현성은 1.0~2.3%로 나타났다. 본 연구에서 확립된 분석법을 적용하여 유통제품 9건에 대해 적용성 검토를 실시한 결과 전체 시료에서 분석이 용이하였으며, 모두 표시기준에 적합함을 확인하였다. 본 연구를 통하여 확립된 GC-MS를 이용한 건강기능식품 중 옥타코사놀 분석법은 향후 공정시험법으로 활용할 수 있으며 국내 건강기능식품의 관리 기반을 강화하는데 기여할 것으로 사료된다.

The purpose of this study was to establish valerenic acid as a marker compound for the standardization of ethanol extract of Valerinan officinalis (valerian) root as a functional health food. We established valerenic acid as a marker compound using HPLC. HPLC was used to quantify the marker compound in the valerian extract after validation of methods with linearity, accuracy, and precision. The specificity for retention time was met by comparative analysis of the valerian extract and standard compound using HPLC. The method showed high linearity of the calibration curve with a coefficient of correlation (R2) of 0.9999. The limit of quantification (LOQ) was 10 μg/mL. The accuracy of measurement was 99.88~ 00.68% and the relative standard deviation (RSD) value was 0.59%. In addition, our analytical method yielded a 29% mean content of valerenic acid in the valerian ethanol extract. These results indicate that the established HPLC method facilitated the determination of marker compounds in the valerian extract for the standardization of health functional foods. Key words: Valerinan officinalis, valerenic acid, HPLC, validation, functional health food

Functional foods for health are often consumed among adolescents. To investigate functional food usage, this study surveyed 326 students aged 14-16. 56.3% of the subjects answered that they were interested in health. More than 76% of the subjects thought themselves to be very healthy. Users of functional foods made up 56.7%, and among all types of functional food, probiotic supplements were taken most frequently, followed by nutritional supplements>red ginseng products>enzyme containing products. Most users of functional food got the information on functional food through family (71.3%). 50.3% of users experienced the effectiveness of the functional food when they supplemented. After use, repurchase intent was 58.1%. In order to investigate the relationship between academic stress and functional food usage, groups were classified as stressful (n=124), intermediate (n=122), and low stress (n=84) according to self-perception about stress. There was no significant difference in the intake of functional foods according to academic stress. However, in the intermediate stress group, academic stress index was improved in the users of functional foods. In conclusion, the results of this study can be used to develop functional foods in the future, possibly helping to prepare nutrition education materials, reducing academic stress among adolescents with middle stress.

The aim of this study was to forecast the future Health Functional Food (HFF) market trends of Korea by studied about policies and market situation in Japan. The HFF is a food that is given an additional health function. In contrast with traditional foods, the HFF has unique characteristics in that it can be produced under the political regulations. The market size of the HFF was growing gradually due to the increased of an aging population and consumer need for high quality and diversified foods. The reason why the HFF market in Japan grew up earlier than other countries, Japan was the first country that institutionalized the HFF policies. Therefore, in this paper, by examining the policies and the market situation of Japan through literatures, we forecasted upcoming political and market situational changes of Korea’s HFF market. We noticed that consumers needs about the HFFs which diversified, confidently ensured safety and ingredients will increase. In this regard, the government will reorganize legislation in order to increase the confidence of the safety of HFFs. In addition, the market size of domestic HFFs which are produced by major food companies will grow.

건강기능식품에서 이산화규소 분석 방법을 확립하기 위하여 산(불산과 붕산)분해를 이용한 ICP-OES 방법을 수행하였다. 이 방법의 검출한계와 정량한계는 각각 0.07 mg/ L, 0.20 mg/L 이었다. 검량선은 0.2~20.0 mg/L의 농도범위 에서 우수한 직선성(r2 0.99)을 보였다. 글루코사민 제품에 이산화규소 0.4, 1.0, 2.0% (w/w)를 첨가하여 시험한 결과 90.22~94.14%의 회수율과 0.72~1.67%의 정밀성을 나타내었다. 확립된 방법으로 시중에 유통되는 건강기능식품 11 품목의 이산화규소 함량을 분석한 결과 0.02~1.80% (w/w) 로 나타났다. 이 결과는 건강기능식품에 이산화규소의 사 용기준 2% (w/w) 이하를 만족하는 결과로 시험한 제품들 은 규격에 적합하였다. 따라서 본 연구에서 확립된 이 방법은 건강기능식품 중 이산화규소를 쉽고, 빠르게 분석할 수 있으며, 건강기능식품 중 이산화규소 함량 분석에 효율 적으로 사용될 수 있다

본 연구에서는 1차 농수산물 및 축산물의 기능성 표시광고를 제한하는 법 제도적 제한요인을 탐색하 였다. 우선 이를 위해 선행연구들을 참조하여 농수산물의 품질 및 안전성과 직결되거나 식품표시와 관 련된 법령인 양곡관리법, 축산물위생관리법, 식품위생법, 건강기능식품에 관한 법률, 시행령, 시행규칙 및 표시광고 관련 조항들을 고찰하였다. 법 조항의 적용 시 법률간 관리대상 식품 형태 및 범위의 모호 성, 규제대상 영업자 모호한 설정이 농수산물 기능성 표시광고의 주요 제한요인으로 판단된다. 또한 법 령 주관부처 별 중복규제 가능성, 행정처분 기준의 모호성 등이 확인되었다. 본 연구를 통해 농식품의 기능성 표시광고 제한요인을 도출하였으며, 이는 1차 농수산물 경쟁력 강화를 위한 정책 기초자료로 활 용될 수 있다.