Tuberculosis (TB) is a significant disease for both humans and animals worldwide. The genus Mycobacterium includes several species that cause TB disease in humans and other animals. Amongst the members of the Mycobacterium tuberculosis complex (MTC), M. tuberculosis is mainly a human pathogen, whereas M. bovis has a broad host range and is the principal agent responsible for TB in domestic and wild mammals. M. bovis also infects humans, causing zoonotic TB through ingestion, inhalation. M. bovis accounts for only a small percentage of the reported cases of TB in humans. In recent years, TB in farmed deer has become a disease as public health importance in several countries. Nowadays, there has been rapid outbreak of bovine TB in cattle and deer in Korea. Investigations are needed to elucidate the relative importance of M. bovis on TB incidence in humans, especially in Korea where bovine TB remains a problem. Also, the human sources as the cause of animal infection, M. tuberculosis from the farm workers also important for TB control of animals in Korea. Differentiation between the causative organisms may only be achieved by sophisticated laboratory methods involving bacteriological characteristics, biochemical properties, and routine resistance to pyrazinamide (PZA). M. bovis shows inherently resistant to PZA whereas M. tuberculosis is susceptible to PZA. In this study, we developed a multiplex-PCR assay based on a 12.7-kb fragment for the differential detection of M. bovis and M. tuberculosis. A total of 131 M. tuberculosis complex isolates were randomly obtained from cattle and deers that were PPD positive. they all yielded M. bovis. M. tuberculosis was not isolated from animals. and, a total of 25 M. tuberculosis complex isolates which is resistant to PZA were obtained from patient. PZA resistant MTC in humans was caused entirely by M. tuberculosis. The multiplex-PCR protocol was highly species-specific and time saving for identification of M. bovis and M. tuberculosis. This multiplex-PCR assay will be easily used as a routine monitoring tool in veterinary and medical laboratories.

Avian influenza (AI) and Newcastle disease (ND) distress a variety of avian species, especially domestic poultry. Severe syndromes are caused by highly virulent specific virus strains termed highly pathogenic AI and velogenic ND viruses, which are potential agrobioterrorism agents. This outbreak emphasizes the need for continuing cooperation between public health and veterinary medical communities in controlling AI and ND when it has a zoonotic potential. Up to date, the stamping out and burying system were applied for controlling methods against these highly infectious diseases in the ordinary way, however these methods had many environmental problems, including leachat and effluvium. Thus, we assessed that sterilization effect of AI and ND virus dependent on several treatment conditions, such as autoclaving time and cutting types of chicken. As a result, we found that the cutting type of chicken meat revealed a reduced HA titer (20) against both of AI and ND virus after 10 min of autoclaving, while whole chicken showed same titer after 30 and 60 min. Therefore, we propose that the conditions of treatment on infected chicken should be developed for convenient, affordable, and effective prevention methods against for AI and ND.

하절기 온실 내 온도와 상대습도를 동시에 제어할 목적으로 압축공기를 이용한 이류체 분무 포그 냉방 을 도입한 유리온실에서 측창의 개폐수준이 온실내의 온도와 상대습도 제어에 어떠한 영향을 주는지 분석하였다. 제어과정은 온실 환경변수들을 측정하고, 이를 반영하여 온실 내 수분평형방정식과 열평형방정식을 정립한 다음, 이 방정식들을 프로그램으로 코딩하여 각 사이클의 천창 개폐량과 포그 분무량을 결정하고 제어하였다. 온실의 온습도 제어목표는 온도와 습도를 각각 28, 75%로 설정하였고, 실험은 측창이 전혀 열리지 않은 0%, 50% 열린 경우, 100% 열린 경우 세 가지 모드를 비교분석하였다. 제어실험결과 온실 내 건구온도의 평균은 모드에 따라 각각 28.2, 27.2 26.3℃로 나타났으며, 표준편차는 0.4∼0.8℃이 었다. 상대습도는 측창을 완전히 닫은 모드인 0%의 경우 평균 76.3% 정도로서 목표 상대습도인 75%에 상당히 근접해서 제어되고 있었으며, 표준편차가 2.1%로서 다른 모드에 비해 상당히 안정된 범위에서 유지되는 것으로 나타났다. 0%의 모드에서 가장 목표온도로 잘 제어되고 있는 것으로 나타났다.

Salmonellosis constitutes an important public health problem in both developing and developed countries, including Korea. The aims of present study were to investigate the serovar and antimicrobial resistance of Salmonella isolated from food animals and animal products in slaughterhouses and farms. A total of 323 Salmonella were isolated from food animals (n=277) and meats (n=46) during 2010. Of the isolates, 21 different serovars were identified. The predominant serovars were S. Rissen (35%) and S. Montevideo (24.3) in healthy pigs, while S. Enteritidis (25.5%) in healthy chicken. S. Typhimurium (88.8%) was predominant in disease pigs, while S. Gallinarum (29.2%) and S. Montevideo (26.9%) were in diseased chickens. Among meat samples, S. Typhimurium (57.1%) was the most common serovar in pork but S. Enteritidis (38.7%) and S. Montevideo(32.3%) were in chikcen meats. Analysis of antimicrobial resistance patterns revealed that 20.7% of the isolates were sensitive to all the 15 drugs tested. The isolates were frequently resistant to nalidixic acid (47.7%), tetracycline (38.4%), streptomycin (33.7%), and ampicillin (32.8%). The resistance to quinolone and 3rd generation cephalosporins was higher in chicken and chicken meat isolates. Of the 323 isolates, 174 (53.9%) were resistant to one or more CLSI subclass, and 117 (36.2%) showed multiple-resistance. Our findings showed that multiple resistant Salmonella organism are widespread in animals and animal products in Korea. To prevent the transmission or exposure for consumers of antimicrobial resistant Salmonella, policies and guidelines aiming at prudential use of critical antimicrobials for humans are needed.

Campylobacterosis is the most common food borne bacterial disease in many countries. Food animals and animal products are considered to be the reservoir of the Campylobacter species. The aim of this study was to investigate the antimicrobial resistance of Campylobacter spp. from food animals and raw meats in slaughterhouses. A total of 90 Campylobacter jejuni (C. jejuni) and 127 Campylobacter coli (C. coli) were isolated, for which antimicrobial susceptibility was examined using broth dilution method. Resistance to macrolide antimicrobials was higher among C. coli isolates than among C. jejuni. Among both C. jejuni and C. coli isolates, the most frequently observed resistance was to nalidixic acid, ciprofloxacin, and tetracycline. No erythromycin resistance was observed among C. jejuni isolates from cattle, pig and beef. However, 28.3% (n=13) and 25% (n=3) of C. coli isolates from pigs and pork showed resistance to erythromycin, respectively. The predominant profile of multiple resistance among C. jejuni and C. coli isolates was ciprofloxacin/tetracycline/nalidixic acid resistance (46.7%) and ciprofloxacin/nalidixic acid resistance (31.5%), respectively. This finding has important implication for food safety and public health.

본 연구에서는 농촌 소하천의 적정 환경유지용수 산정을 위한 기초조사로 경상남도 고성군 하이면 와룡 리에 위치한 봉현저수지를 수원으로 하는 봉현천과 하이저수지를 수원으로 하는 석지천을 대상으로 시기 적 우기이면서 농번기인 2010년 5월에서 10월까지 총 6회에 걸쳐 18개 지점을 선정하여 하천조사 및 유 량 모니터링을 실시하였다. 그 결과 선행 강수량이 많을 경우 하천의 전 구간에 유량이 증가하였고, 선행 강수량이 적거나 없는 경우에는 계측이 불가능할 정도로 하천이 메말라 있었다. 각 하천의 상류부의 경우 에는 항시 일정량의 유량을 확인 할 수 있었는데 이는 댐 침윤에 기인한 것으로 추정되었다. 유량 프로파 일을 작성한 결과 봉현천 하류부 2,000 m 지점과 3,500 m 지점, 석지천 하류부 1,200 m 지점에서 주변 참다래 과수원의 관개용수 양수, 참다래 보관 및 선별공장에서의 취수 및 관측지점 상류부의 연속적인 보 의 영향으로 유량이 감소하는 것을 확인할 수 있었고, 봉현천 하류부 4,200 m 지점에서는 유량이 증가하 였는데 이는 배수로를 통한 유출수의 유입에 의한 것으로 추정되었다. 향후 지속적이고 장기적인 현장 모 니터링을 통하여 농어촌환경용수 공급을 위한 적정 유지유량을 산정하는데 기초자료로 활용할 수 있을 것 으로 사료된다.

Staphylococcus aureus is an important food-borne pathogen, which is present on the skin and mucosa of animals. Some of the S. aureus strains are causative agent of food poisoning syndrome. The aim of this study was to investigate the antimicrobial resistance of S. aureus isolates from raw meats in slaughterhouses during 2010. From 17,874 raw meat samples tested, a total of 190 S. aureus were isolated, for which antimicrobial susceptibility to 17 agents was examined using broth dilution method. Among isolates from beef, chicken and pork, 20 (51.3%), 20 (24.7%) and 9 (12.9%) were sensitive to all antimicrobials tested, respectively. Isolates from pork and chicken meats showed much higher resistance, compared to isolates from beef. Penicillin resistance was the most frequent among isolates from beef (35.3%) and pork (75.7%), while tetracycline resistance was among those from chicken meats (48.1%). A total of 3 methicillin resistant S. aureus (MRSA) were detected from beef (5.1%, 2/39) and pork (1.4%, 1/70). Although the prevalence of MRSA was low, the presence of antimicrobial resistant S. aureus such as MRSA suggests that further investigation and strict surveillance on MRSA and antimicrobial resistance are needed.

아세틸콜린 분해효소(acetylcholine esterase, AChE) 억제제는 아세틸콜린 함량을 높여 콜린성 neuron 을 활성화함으로써 기억 능력의 개선 및 치매 개선을 가져와 현재 다양한 AChE 억제제들이 개발되어 사용되고 있다. 본 연구에서는 AChE에 대한 억제 활성을 갖는 천연물을 다양한 식물추출물 및 에센스오일로부터 탐색하였으며, 탐색한 추출물의 scopolamine으로 기억손상을 유발한 쥐의 기억력 개선 활성을 치매 치료제로 사용하고 있는 donepizil과 비교 분석하였다. 그 결과 자몽(Citrus paradisi) 유래의 에센스 오일이 AChE 억제 활성이 가장 높아 20 ug/ml의 농도로 처리하였을 때 90% 이상의 효소 억제 활성을 나타내었다. 수동회피 실험 결과, 자몽 유래의 에센스오일(100 mg/kg, p.o.)을 투여한 쥐는 치매 치료제로 사용하고 있는 donepizile (0.5 mg/kg)을 투여한 쥐와 유사한 latency time을 나타내어 인지기능이 개선되었다. 또한, 수중미로 시험 결과, 자몽 유래 에센스오일(100 mg/Kg, p.o.)을 투여한 쥐는 donepizile(0.5 mg/kg)을 투여한 쥐와 유사한 latency time을 나타내어 인지기능이 개선되었다. 이상의 결과로부터 자몽 유래 에센스오일은 매우 효과적으로 기억력을 개선하여 인지기능을 개선해 줄 수 있는 안전하고 효과적인 후보물질이라고 사료된다.

인천 연안의 심하게 오염된 갯벌로부터 강력한 세포외 알카리성 단백질 분해효소를 생산하는 호알카리 성 Bacillus clausii I-52를 분리하였으며, 이 균주로부터 알카리성 단백질 분해효소의 유전자를 cloning 하여 서열 분석을 하였다. Chromosome 서열이 완전히 밝혀진 Bacillus subtilis의 서열을 기초로 하여 알카리성 단백질 분해효소 및 promoter를 포함하도록 primer를 고안하여 PCR을 수행하여 2,277 bp의 DNA 단편을 얻었으며 BLAST 분석 결과 29 개의 아미노산으로 이루어진 signal peptide, 77 개의 아미 노산으로 이루어진 propeptide 및 275 개의 아미노산을 갖는 활성형의 BCAP으로 구성된 총 381 개의 아미노산을 코딩하는 1,143 bp의 open reading frame을 확인하였다. 활성형 BCAP의 N-말단 아미노산은 Ala이며, 분자량 및 pI 값은 각각 27698.7 Da과 6.30으로 계산되었다. 아미노산 상동성을 분석한 결과, B. subtilis 유래의 nattokinase precursor 및 B. subtilis BSn5 유래의 subtilisin E precursor와 99%의 서열 상동성을 나타내어 B. clausii I-52 유래의 BCAP은 subtilisin 계열의 단백질 분해효소임을 확인하였다. E. coli BL21(DE3)에서 발현한 재조합 BCAP는 N-Suc-Ala-Ala-Pro-Phe-pNA 를 효율적으로 분해하였다. Refolding한 재조합 BCAP은 전형적인 serine protease inhibitor인 PMSF에 의하여 강하게 효소 활성이 억제됨으로써 serine protease 계열의 단백질 분해효소임을 알 수 있었다.

This study examined the effect of active and passive listening condition on young children's musical understanding of familiar and unfamiliar melody through researcher-invented notation. All subjects in this study were Korean children from 3 to 5 years of ages in a private prekindergarten in Seoul Korea. A pre-and posttest experimental design guided this study, which was conducted for five consecutive weeks, including three treatment sessions between pre-and posttest sessions in summer of 2009. The following research questions for the study were posed: 1) Is there a significant difference in young children's apparent understanding of tempo when comparing active and passive listening condition? and 2) Is there a significant difference in young children's apparent understanding of tempo according to familiarity of the melody? Listening conditions (independent variable) in this study were the presence or absence of body movement while children listened to short musical examples. The children's apparent musical understanding of tempo (independent variable) was captured through children's guided drawings that indicated where they understood tempo changes to occur in the music. The drawings were evaluated by three independent judges who scored four drawings for each child: for familiar and unfamiliar melodies in both the pre-and posttest. A two-way ANOVA was used to analyze the scores. The results revealed that there is no statistically significant difference between treatment and control groups, between familiar and unfamiliar melody, and no difference with the interaction of group and melody familiarity. Therefore, the null hypotheses for this study were accepted.

본 연구는 콘덴싱 보일러 내부에 들어가는 원심 터보형 송풍기의 성능 향상 및 그 유동특성을 파악하고자 하였다. 보일러는 연소를 위해 반드시 필요한 가스와 공기의 양이 조절되어야하며, 이를 위해 송풍기는 보일러 내부의 한정된 영역을 차지한다. 한정된 영역에서 최적의 효율을 얻기 위해서는 케이싱 및 임펠러의 형상을 변경하는 것이 일반적으로 사용되며, 형상변화에 따른 입/출구압력, 유량, 임펠러의 토크 등의 성능특성을 파악하였다. MRF(Multiple Rotating Frame)기법 및 k-ù SST 난류 모델을 적용하여 회전 임펠러에 대한 수치해석을 수행하였으며, 형상의 변경에 따라 송풍기의 성능특성 및 최적화된 설계형상을 제안하였고, 압력측정실험을 통해 그 결과를 검증하였다.

The bracket sets which are supporting the weight of the roof, has to meet both requirements which are structural functions and act as an ornamental element. Therefore the bracket sets differ in many types from time and space and has done an important role in the history of architecture with many studies being presented. The first form of the bracket set has been found in the bronze table relics in zhōng shān wáng líng (中山王陵). Through the Eastern Han dynasty (A.D. 25-220) it became more specific in the shique (石闕) and huaxiangshi (畵像石) in the Han dynasty (206 B.C.-A.D. 220). Afterwards, as Buddhism was introduced to China, the bracket construction techniques shown in the Mogao Caves, Yungang Grottoes, and Longmen Grottoes has given much help for understanding the building techniques of wooden architecture. Especially the Xia-ang structure seen in the Mogao caves shows a vast development in wooden structure and a typical building would be the main hall of Fo Guang Shan monastery in Mt. Wutaishan from the Tang dynasty (A.D. 618-907). This accumulated techniques is inscribed in the 'Ying Zao Fa Shi (營造法式)' wooden structure designs which was published during the Northern Song dynasty (A.D. 960-1127) and many buildings were constructed following this technique after the publication. During this period, it is assumed that Baek-jae (B.C.18-A.D.660) in the Korean peninsula also used the Xia-ang technique, but there havn't been many studies on this field. In this thesis it is introducing the development of the building techniques and structural features of the Xia-ang wooden architecture during the Tang and Song dynasty.

The purpose of this study is to understand Liszt's music using the intertextuality theory of Kristeva. The intertextuality theory is based on post-structuralism that there might not be a purely new work on any composer's. It is a mere fabricated thing which is reflected by unconsciousness of composer's experiences. Liszt is one of the most influenced composers by literature, philosophy, and art. Especially he composed not a few piano works on poets. 《Mazeppa》is the one he composed using poem of Victor Hugo. It comprised intertextual elements of carnaval languages and 'menippea' which Kisteva suggested for analyzing literature. The other conception of 'chora sémiotique' was also involved in his work. As the result of this study, the conversion of diachrony into synchrony in turn and the distinction between carnaval languages and menipea were found to be able to apply to music not only literature. This result would suggest a new significant method, as an alternative of traditional method, in music understandings.

It is known that the content of saturated fatty acids methyl ester (SFAME) affect the pour point of biodiesel at low temperature. In this study, biodiesel (BD) was produced from beef tallow (TAL) by alkali catalyst. To reduce the saturation in BD, acetone fractionation was applied. Besides, TAL was also solvent-fractionated to reduce the saturated fatty acid (SFA) content for further producing BD. With acetone, TAL or TAL methyl ester (5:1 v/w) were fractionated at 10, 0, -10, and -15℃, respectively. At -10℃, 17.35% of SFA was observed in fractionated TAL (liquid part, -10TAL) when 5:1 solvent ratio was used for 24 hr. Under the same condition, fractionated BD (liquid part, -10BD) showed SFA (33.14%) with 78wt % yield. Also, fractionation of BD with different concentration of crystallizer 209 (0.1, 0.5, and 1%) along with different time (2, 6, 12, and 24 hr.) was observed. The best condition for reducing the SFA was 0.5% of crystallizer 209 addition for 12 hr of fractionation time at -10℃, in which 30.14% of SFA content was observed in BD (liquid part). Among different crystallizer, ps 66 showed the least content of SFA content (23.28%) in BD after fractionation (-10℃ and 24 hr) with 0.5wt% addition.