A strain of Alexandrium species was established by isolating cells from Jangmok Bay, Korea. Its morphology and molecular phylogeny based on LSU rRNA gene sequences were examined. In addition, growth responses of this Alexandrium species to changes in temperature, salinity, and nutrient concentrations were investigated. This Alexandrium species from Jangmok Bay had a ventral pore on the 1′, which was morphologically consistent with previously described Alexandrium tamarense and A. catenella. Phylogenetic analyses revealed that this isolate was assigned to A. pacificum (Group IV) within A. tamarense species complex. In growth experiments, relatively high growth rates and cell densities of A. pacificum (Group IV) were observed at 15°C and 20°C. This species also grew under a wide range of salinity. This indicates that this Korean isolate of A. pacificum (Group IV) is a stenothermic and euryhaline species. In growth responses to changes in nutrient levels, enhanced growth rates and cell densities of A. pacificum (Group IV) were observed with additions of nitrate and phosphate. In particular, rapid uptakes of phosphate by A. pacificum (Group IV) were observed in experimental treatments, indicating that the increase in phosphate concentration could stimulate the growth of A. pacificum (Group IV).

Anthropogenic disturbances on freshwater ecosystem are known to degrade biodiversity, especially on fish assemblage. In this study, we have conducted fish surveys to identify impact of a bridge construction on fish assemblages. A total of eight study sites were surveyed in the Danjang and the Dong Stream in southern part of South Korea from June to November in 2021. The fish samplings were carried out five times, using cast-nets (10×10 mm mesh size), scoop-nets (4×4 mm, 5×5 mm mesh size), set-nets (10×10 mm mesh size), and fish traps (3×3 mm mesh size), along with the Stream/River Ecosystem Survey and Health Assessment by the Ministry of Environment of Korea and basic water quality measurement. Also, we applied the species diversity index and length-weight relationship regressions on certain species to identify interspecific growth rate differences in accordance with study sites. As a result, a total of 782 individuals, 23 species and 10 families were collected. The dominant species was Zacco Koreanus and relative abundance was 50.89%. When applying the length-weight relationship regressions on certain species, the ‘b’ value for Z. Koreanus was lower at the downstream points than at the upstream points of the construction site. In addition, when comparing to the results of the past survey, relative density of demersal fish at the upstream and downstream points decreased from 26% to 1.4%, and from 18% to 6.3%, respectively. In conclusion, it is considered that bridge construction negatively affects the habitat of fishes, especially on demersal fishes. Therefore, appropriate conservation efforts such as installation of silt protector and sand sedimentation pond are needed to alleviate the disturbance in habitat such as occurrence of turbidity and destruction of micro-habitats.

Coffee is the most popular beverage in the world and various pollutants, including volatile organic compounds (VOCs), are emitted from the coffee manufacturing workplace (roasting process). In this study, we analyzed the characteristics of VOCs emissions from roasted Arabica coffee bean powder using a VOCs emission chamber with a PTR-ToF-MS. The emission test was maintained under constant temperature (20 ± 2oC) and humidity (50 ± 5%) conditions. As a result of the emission test, most of the target compounds had a high concentration in the initial period, and decreased emissions as time lapsed. Acetaldehyde showed the highest concentration and was initially 78 ppm during the test period. Acetaldehyde was followed by propionic acid at 61 ppm, propanal at 51 ppm, and isobutanal at 50 ppm. As a result of comparing the occupational exposure limits (OELs) of individual VOC emitted during the coffee roasting process, the OELs of four substances, including acetaldehyde, propionic acid, acetic acid, and pyridine were identified. Of all four substances, only pyridine exceeded the OELs, and the other compounds had levels of 10% to 30% of the OELs.

The purpose of this study is to evaluate seismic performances of a modular house system developed by a simple 4-clip fastening method and double metal assembly made of lightweight metals. In order to evaluate structural and non-structural seismic performances of the system. Shaking table test was carried out with full-scale modular units, and a nonlinear pushover analysis was performed to obtain suitable seismic responses for story drifts, displacements, force resistances and dynamic properties of the system. Through 3D analysis and shaking table test, the current method of lightweight modular metal unit assembly and systems with seismic performance of a 4-clip fastening type modular house were demonstrated safe and effective to seismic design.

In this paper, the instability of the domed spatial truss structure using wood and the characteristics of the buckling critical load were studied. Hexagonal space truss was adopted as the model to be analyzed, and two boundary conditions were considered. In the first case, the deformation of the inclined member is only considered, and in the second case, the deformation of the horizontal member is also considered. The materials of the model adopted in this paper are steel and timbers, and the considered timbers are spruce, pine, and larch. Here, the inelastic properties of the material are not considered. The instability of the target structure was observed through non-linear incremental analysis, and the buckling critical load was calculated through the singularities and eigenvalues of the tangential stiffness matrix at each incremental step. From the analysis results, in the example of the boundary condition considering only the inclined member, the critical buckling load was lower when using timber than when using steel, and the critical buckling load was determined according to the modulus of elasticity of timber. In the case of boundary conditions considering the effect of the horizontal member, using a mixture of steel and timber case had a lower buckling critical load than the steel case. But, the result showed that it was more effective in structural stability than only timber was used.

큰앵초의 특성조사는 소백산(연화봉), 주흘산(주봉), 덕유산(삿갓봉), 가야산(두리봉), 지리산(노고단) 등 5개 지역을 대상으로 2018년 4월부터 2020년 11월까지 실시하였다. 큰앵초의 높이는 23.1∼31.3㎝이고, 평균 26.8㎝이며, 잎의 수는 2.9∼4.7개이고, 평균 3.8개이다. 엽신의 길이는 6.2∼7.6㎝이고, 평균 7.1㎝이며, 엽신의 너비는 7.8∼9.3㎝이고, 평균 8.7㎝이며, 엽병의 길이는 10.9∼16.5㎝이고, 평균 13.5㎝이다. 잎의 길이와 폭의 비율은 0.79∼0.82로 광타원형이다. 엽연의 형태는 결각이 둔하고 불규칙적인 것과 예리하고 깊으며 규칙적인 것으로 나타났다. 뿌리의 길이는 5.9∼10.9㎝이고 평균 7.2㎝이며, 뿌리 수는 27.6∼48.2개이고, 평균 37.7개이다. 개화기는 5월말∼6월 초이며, 꽃의 수는 5.4∼10.1개이고, 평균 7.2개이다. 화관너비는 21.7∼26.2㎜이고, 평균 23.4㎜이다. 열매의 길이는 1.3㎜∼2㎜이고, 평균 1.8㎜이며, 종자의 수는 10개∼18.2개이고, 평균 14.2개이다. 조사지의 상층우점종은 신갈나무이고, 중층은 당단풍나무, 노린재나무, 함박꽃나무 등이며, 하층은 철쭉, 진달래, 미역줄나무, 국수나무 등으로 나타났고, 종다양도는 0.7534∼0.9950으로 나타났다. 조사지의 토양 pH는 4.1∼4.6이고 염기포화도는 3.18%∼9.09%로 나타났다. EC는 0.18∼0.7dSm-1으로 나타났고, NaCl은 0.003∼0.006%로 나타났다.

PURPOSES : A methodology for estimating micro-emission factors using vehicle trajectory data collected from navigation and DTG devices and basic emission factors for each vehicle type of the MOtor Vehicle Emission Simulator (MOVES) is presented. The methodology can calculate micro-emissions based on only the traffic volume and average speed for each vehicle type. METHODS : Cluster analysis was performed by accumulating the trajectories of individual vehicles on a specific road section into speed groups in which vehicles drove with the same range of average speed. Then, the micro-emission factors were estimated for each speed group. RESULTS : Using the vehicle trajectory data revealed that the emissions calculated from micro-emission factors estimated by the proposed methodology were similar to the sum of the emissions calculated from the vehicle trajectories for each vehicle. CONCLUSIONS : The micro-emission factor database for each road type and vehicle type proposed in this study should be useful for estimating vehicle emissions on the road. The proposed method can calculate emissions in the same way as the macroscopic analysis method, using the traffic volume, average speed, and link length.

PURPOSES : The purpose of this study was to reduce greenhouse gases and prevent potholes on roads by evaluating the performances of hot and warm mixed asphalt mixtures. METHODS : Quality tests were conducted using an appropriate aggregate ratio of the asphalt mixture. The tests for comparing the warm mixed asphalt mixture are the indirect tensile strength and toughness, Marshall stability and flow, tensile strength ratio, and dynamic immersion test. A performance evaluation was conducted using a mixture that satisfied the quality test results. A performance evaluation test was also conducted using the dynamic modulus and Hamburg wheel tracking test. To analyze the performance based on the amine content, the performance was compared with that of a hot mixed asphalt mixture. RESULTS : All tests for the mixture results satisfied the standard values. The optimal amine content was analyzed through the high and low frequencies of the dynamic modulus test results and stripping inflection point with the final rut depth of the Hamburg wheel tracking test. The dynamic modulus test results demonstrated better crack resistance and plastic deformation when a high amine content ratio was used. The Hamburg wheel-tracking test showed water resistance and plastic deformation resistance. The test results of the Hamburg wheel tracking indicated better deformation resistance and water resistance when a high amine content ratio was used. CONCLUSIONS : The plastic deformation and crack resistance increased with an increase in amine content. Analysis of the comprehensive test revealed that the optimal amine content was between that of additives B(50%) and C(65%). Tests with a granular amine content are planned to confirm the specific components. Also planned are a simplified viscoelastic continuum damage test and a semicircular bending test to evaluate the performance better.

Novel Ni- and Fe-based alloys are developed to impart improved mechanical properties and corrosion resistance. The designed alloys are manufactured as a powder and deposited on a steel substrate using a high-velocity oxygen-fuel process. The coating layer demonstrates good corrosion resistance, and the thus-formed passive film is beneficial because of the Cr contained in the alloy system. Furthermore, during low-temperature heat treatment, factors that deteriorate the properties and which may arise during high-temperature heat treatment, are avoided. For the heattreated coating layers, the hardness increases by up to 32% and the corrosion resistance improves. The influence of the heat treatment is investigated through various methods and is considered to enhance the mechanical properties and corrosion resistance of the coating layer.

Asthma is a chronic inflammatory disease characterized by recurring symptoms, airflow obstruction, and bronchial hyper-responsiveness. The onset of asthma for most patients begins early in life, and current asthma treatment with anti-inflammatory agents can have adverse effects, eventually leading to impaired quality of life. In the pathogenesis of asthma, macrophages and basophils play a vital role during progression. Macrophages not only induce inflammation by secreting inflammatory cytokines but also promote DNA damage and mucus production through nitric oxide (NO) production. Basophils enhance eosinophil recruitment and aggravate asthma through the FcεRIα receptor with high affinity for histamine and IgE. Therefore, in this study, we investigated whether the activation of macrophages and basophils is suppressed by the individual extracts of 28 natural products. RAW 264.7 cells (mouse macrophages) were treated with the natural products in LPS, and 4 natural product extracts resulted in decreased NO production. In β-hexosaminidase assay using RBL-2H3 cells (rat basophils), 19 natural product extracts decreased β-hexosaminidase production. In NO production and β-hexosaminidase assay using macrophages and basophils, 3 natural product extracts (Plantago asiatica, Centella asiatica, and Perilla frutescens var. japonica) significantly inhibited NO production and β-hexosaminidase release. Overall, we examined the inhibitory effects of 28 natural product extracts on macrophage and basophil activity, and the findings demonstrated the potential of natural product extracts for treating asthma and macrophage- and basophil-related diseases.

Benthic macroinvertebrates are important ecological and environmental indicators as primary or secondary consumers, and therefore are widely used in the evaluation of aquatic environments. However, there are no comprehensive river ecosystem monitoring surveys that link the major physicochemical water quality items with benthic macroinvertebrates in urban streams. Therefore, this study investigated the distribution characteristics of benthic macroinvertebrates and physicochemical water quality items (17 items) in Yangjaecheon and Yeouicheon from 2019 to 2020. At the same time, by applying Spearman’s rank correlation analysis and nonmetric multidimensional scaling (nMDS) analysis in the water quality data and biotic index, we tried to provide basic data for diagnosing the current status of river ecosystems in major urban rivers in Seoul. Based on the study results, a total of 39 species and 3,787 individuals were identified in Yangjaecheon, the water quality (based on BOD, TOC, and TP) of Yangjaecheon was higher than Grade Ib (good), and the BMI using benthic macroinvertebrates appeared as Grade C (normal) at all the sites. In Yeouicheon, a total of 51 species and 4,199 individuals were identified, the water quality (based on BOD, TOC, TP) was higher than Grade Ib (good) similar to Yangjaecheon, and the BMI of both Upstream and Saewon bridge was Grade B (good), while Yeoui bridge was Grade C (normal). Overall, analysis results for the distribution of benthic macroinvertebrates by a nonmetric multidimensional scaling method showed no significant difference between the two streams (p=0.1491). Also, significant environmental variables related to benthic macroinvertebrates distribution were determined as water temperature and DO. On the other hand, the results of the correlation analysis between biotic index and major water quality items confirmed that R1 and BMI could be used for on-site urban river water quality evaluation.

글루탐산은 식물의 생장과 발달에 중요한 역할을 하는 필수 아미노산의 전구체이며, 저온 보호 물질로 이어지는 생합성 경로를 자극하여 저온 피해를 줄이는 생물자극제 중 하나이 다. 본 연구에서는 저온 스트레스 조건에서 글루탐산 엽면 처 리가 배추에 미치는 영향을 평가하였다. 글루탐산 2가지 엽면 시비 농도(0 및 10mg·L-1)와 3가지 주/야간 온도 수준(11/－1 °C extremely low, E; 16/4°C moderately low, M; 21/9°C optimal, O)을 결합하여 6개의 처리가 수행되었다. 글루탐산 의 엽면 처리는 정식 후 10일에 1회 살포하고, 글루탐산 처리 직후 온도 처리는 최대 4일 동안 실시하였다. 처리 4일 후, ABA, PA, DPA 및 ABA-GE 함량은 M 처리에서 Glu 0 처리 보다 Glu 10 처리에서 함량이 더 높았다. Glucose 함량은 E 및 Glu 10 처리에서 가장 높은 반면(52.1mg·100g-1 dry weight), fructose 함량은 O 및 Glu 0 처리에서 함량이 가장 높았다 (134.6mg·100g-1 dry weight). GLP, GBS, 4MGBS, GNBS 및 GNS 함량은 E 및 Glu 10 처리에서 모든 처리 중 가장 높았 다(0.72, 2.05, 1.67, 9.40 및 0.85μmol·g-1 dry weight). 처리 2일 후 E 및 Glu 10 처리의 PA와 DPA함량에서 급격한 변화 를 확인하였고, 몇몇 개별 glucosinolate 함량(GLP, GBS, 4MGBS, GNBS 및 GNS)은 저온과 글루탐산 처리에 따라 유 의적 차이를 확인할 수 있었다. 또한, fructose는 glucose 대신 fructan을 에너지원으로 사용하였기 때문에 처리 4일후 E와 M처리에서 O 처리에 비하여 유의적으로 낮은 함량을 보였다. 따라서, 저온과 글루탐산 엽면 처리에 따른 PA, DPA, glucose, fructose 및 개별 glucosinolate 물질들의 변이를 확 인하였지만, 저온과 글루탐산의 효과에 관한 명확한 상관관계를 평가할 수는 없었다. 배추과 작물은 호냉성 채소로서 저 온에 민감하게 반응하지 않고, 대부분 내한성을 가지고 있기 때문으로 판단된다.

대기오염 등의 영향으로 실내 활동 시간이 증가하는 상황에서 뇌기능과 신체활동 측면에서 보다 건강한 실내 생활 을 위한 다양한 방안에 대한 고려가 절실한 실정이다. 본 연구는 실내 산소의 농도와 호흡의 방법에 따라 인지능력과 생체신호에 어떤 영향을 미치는지에 대해 연구하여 보다 건강한 실내 일상생활을 제안하고자 한다. 건강한 성인 20 명을 대상으로 공기 전달 시스템을 통해 공급된 공기를 산소마스크를 통해 흡입하도록 하였다. 호흡 방법과 산소 농도에 따른 변화를 확인하기 위해 모든 피험자는 3가지 호흡(비강, 구강, 및 고농도 산소공급과 함께 구강 호흡)을 인지부하자극(눈 감은 휴식기, 눈 뜬 휴식기, 1-back 및 2-back 작업기억 과제) 별로 각각 수행하였다. 자극에 대한 반응시간, 정확도 및 생체신호를 측정하여 호흡에 따른 인지부하별 변화를 분석하였다. 그 결과, 3가지 호흡에서 모 두 인지부하의 증가에 따라 심박동수는 유의하게 증가하면서 인지부하와 높은 상관성이 있는 생체신호임을 확인하 였다. 또한 구강호흡에서는 분당 호흡 횟수가 인지부하증가에 따라 유의하게 증가하는 변화가 나타났으며, 인지활동 시 구강호흡에서 부족한 산소공급을 보완하기 위한 생체신호의 변화를 확인하게 되었다. 반면 고농도 산소공급과 함께 수행한 구강호흡에서는 비강호흡과 같이 분당 호흡 횟수의 유의한 변화는 없는 것으로 나타났다. 이는 고농도 산소공급이 구강호흡과 같이 비효율적인 산소 흡입이나 산소 농도가 부족한 환경에서 이를 보완해 주는 역할을 할 가능성이 있음을 시사한다. 본 연구 결과를 기반으로 후속 연구에서는 자율신경계 변화까지 분석하여 인지 뿐만 아 니라 심리에 미치는 영향까지 종합적으로 규명하고자 한다.

동물복지 향상에 대한 국민적 인식과 요구가 높아진만큼 작년 동물보호법 개정과 지자 체에서도 동물복지팀 신설 등 다양한 활동들이 이루어지고 있다. 말은 동물보호법에 기재 되어 있는 동물 범위에 포함되어 있지만 구체적인 말에 관한 법령은 기재되어 있지 않아 아직까지 국내 경주마에 대한 복지체계는 턱없이 부족하다. 이에 본 논문에서는 국내 경 주마의 육성과 생애를 시작으로 은퇴한 퇴역마까지의 전반적인 삶에 관해 학대 및 도축, 채찍의 측면에서 나누어 해외 사례와 비교 분석한 뒤 국내 경주마 복지의 미비점 및 해결 방안에 대해 고찰하였다. 미국의 경우 2009년 말 학대 방지법과 2011년에는 말 도축 방 지법을 제정하였으며 영국 또한 경주마 관련 법이 존재한다. 1986년 노르웨이에서는 채 찍 사용을 전면 금지하였고 2009년에는 관련 법안을 더 정교하게 개정하였다. 스웨덴 역 시 올해 2022년부터 채찍 사용이 법적으로 제한된다. 이와는 대조적으로 국내 동물보호 법에 말에 관한 사항은 전무하며 이렇기에 다른 국가들에 비해 법적 책임과 복지 측면에 서의 관리 방안들이 미흡하다는 결과가 나타났다. 따라서 국내 경주마의 복지 향상을 위 해서 체계적인 법 규정이 필요한 것으로 보인다.

수의사 보조원은 동물병원에서 동물에 대한 처치, 접수, 보호자 안내, 수납 업무 등을 통해 수의사를 지원하고 보조한다. 이러한 역할을 수행하기 위해서는 적극적 경청과 원활 한 의사소통 능력을 갖춰야 한다. 이것과 관련하여 조사와 분석을 통해 도출된 결과로 인 식의 함양이 필요하지만 현재 이와 관련된 선행연구가 부족한 실정이다. 본 연구는 동물 병원 서비스 이용 경험이 있는 전라북도 반려인의 이용 만족도 현황과 정도를 파악하고 이에 수의사 보조원의 언어적, 비언어적 의사소통 능력이 미치는 영향의 유무를 규명하고 자 한다. 또한, 전반적인 의사소통 능력의 중요성과 영향력을 인식하는 데 목적을 둔다. 2022년 4월 30일부터 2022년 5월 29일까지 온라인 설문조사를 실시하였다. 응답 결과 의 데이터는 빈도 분석, 신뢰도 분석, 상관 분석을 사용하여 인구통계학적 기준으로 분석 하였다. 첫째, 수의사 보조원의 언어적, 비언어적 의사소통 능력의 개념을 정리하기 위한 신뢰도 분석 결과 Cronbach’s alpha 계수는 모두 0.8 이상으로 나타나 신뢰도가 검증되 었다. 둘째, 수의사 보조원의 언어적, 비언어적 의사소통 능력이 동물병원 이용자의 만족 도에 미치는 영향의 여부와 정도를 파악하기 위해 상관분석을 실시하였다. 그 결과 모두 정(+)의 상관관계로 나타났다. 하여, 본 연구는 수의사 보조원의 언어적, 비언어적 의사 소통 능력의 영향력 인식을 통해 중요성 함양하고자 하는 데 의의가 있다.