Aloin [1,8-Dihydroxy-10-(β-D-glucopyranosyl)-3- (hydroxymethyl)-9(10H)-anthracenone]은 알로에에서 추출한 천연 안트라퀴논이다. 다양한 유형의 인간 암세포에서 항산화, 항암 효과가 있는 것으로 밝혀졌지만 인간 대장암 세포 HT-29에서 aloin의 항암 효과는 밝혀지지 않았다. 본 연구에서는 aloin이 인간 대장암 HT-29 세포에서 세포 사멸 작용을 발휘할 수 있는 메커니즘을 조사하였다. Aloin 이 세포 생존율에 영향을 미치는지 알아보기 위해 대장암 세포 HT-29, 흑색종 세포 A375SM, 위암 세포 AGS를 aloin(0, 100, 200, 300 및 400 μM)으로 처리하였을 때, HT- 29에서는 농도 의존적으로 세포 생존율을 감소시켰고, A375SM과 AGS 세포에서는 암세포 생존율의 감소가 보이지 않았다. 이러한 HT-29에서의 세포 생존율 감소가 세포자멸사로 인한 감소인지 확인하기 위해 DAPI stain과 flow cytometry를 실시한 결과 apoptotic body가 유의적으로 증가하고 세포 자멸사가 증가한 것을 확인할 수 있었다. 이와 같은 결과를 바탕으로 aloin이 대장암 세포 HT- 29에서 세포 사멸 관련 단백질 발현 양상에 미치는 영향을 확인하기 위해 western blotting을 실시하였다. Aloin은 Bax, PARP의 분절을 농도 의존적으로 증가시켰고, caspase- 3, -8을 활성화시켰지만, Bcl-2는 대조군에 비해 변화가 없었다. Aloin에 의해 유도된 세포자멸사 기전을 확인하기 위해 MAPK pathway 중 p-p38과 p-ERK의 발현을 확인한 결과, p-p38을 up-regulation시키고 p-ERK의 downregulation을 유도했다. 따라서, aloin은 인간 대장암에서 암 세포 성장 억제 효과 및 암세포 사멸 유도로 암예방 약제로서의 개발 가능성이 있다고 사료된다.

The purpose of this study is to analyze the distribution characteristics of mist spray particle size by devising a rotary mist spraying device to develop the evaporative salt water desalination system. The rotary mist spraying device was consisted of a BLDC sirocco fan, a spinning fan, a fan fixed shaft and a salt water supply device etc. In this study we analyzed the characteristics of spray particle size and distribution according to the variation of sirocco fan surface roughness(Ra, μm), revolutions(rpm) and salt water flow rate(mL/min). When sirocco fan surface roughness( Ra) was in the range of 0.27~7.65 μm, the spray particle size was 0.117~1.360 μm. And then more than 90% of spray particles were found to be less than 0.50 μm. When sirocco fan surface roughness(Ra) was in the range of 12.70~22.84 μm, the spray particle size was 2.51~184.79 μm and more than 98% of spray particles were found to be less than 13.59 μm. To analyze the effect of fan rotation speed on the size and distribution of spray particles, when surface roughness Ra was fixed 0.27 μm and fan rotation speed and salt water flow rate was respectively changed at 3,800~5,600 rpm and 2.77~8.28 mL/min, spray particle size was 0.341~0.541 μm. And when salt water flow rate was 9.74 mL/min and fan rotation speed was 3,800~5,200 rpm, spray particle size was in the range of 29.29~341.46 μm and in case of 5,600 rpm more than 98.23% of spray particles were in the range of 2.51~13.59 μm.

본 연구에서는 chlorogenic acid 이성질체 9종과 arbutin을 분석하기 위하여, 전처리 과정과 UHPLC-MS/MS 동시 분석 조건을 확립하였다. UHPLC-MS/MS에서 C18 column을 사용하여 15분 동안 분석할 수 있는 용매 조성을 만들고, 10가지 물질에 대한 정량, 정성 이온을 선택하여 negative 모드에서 분석하는 동시분석법을 확립하였고 과일류를 대상으로 추출, 진탕 추출, 초음파 추출, 원심분리, 농축 등의 과정을 거치는 전처리법을 개발하였다. 기기분석과 전처리법에 대한 유효성은 특이성, 직선성, 정확성, 정밀성 그리고 검출한계 및 정량한계 등을 통하여 확인하였다. 회수율은 48.1-120.3%, intraday precision (RSD)는 0.4-7.9%, interday precision (RSD)는 0.0-7.2%로 확인되었다. 따라서, 본 연구에서 확립한 동시분석방법은 과일류 중 chlorogenic acid와 arbutin을 효과적으로 분석하는데 유용할 것으로 사료된다.

The purpose of this study is to suggest structural model and analyze design factors for the development of small greenhouse standardization model. The average dimensions of small greenhouse desired by urban farmers were 3.3m in width, 1.9m in eaves height, 2.7m in ridge height, 5.7m in length. The cladding materials for small greenhouse were preferred to glass, PC board and plastic film, framework to aluminum alloy and steel, and heating method in electrical energy. In addition, it was analyzed that small greenhouses need to develop structural model by dividing them into entry-level type and high-level type. The roof type that was used for entry-level type was arch shape, framework was steel pipe, cladding material was plastic film. On the other hand, high-level type was used in even span or dutch light type, framework with square hollow steel, cladding materials with glass or PC board. In consideration of these findings and practicality, this study developed four types of small greenhouses. The width, eaves height, ridges height, and length of the small greenhouses of even span type, which were covered with 5mm thick glass and 6mm thick PC board were 3m, 2.2m, 2.9m, and 6m, respectively. The small greenhouse of dutch light type covered with 5mm thick glass was designed with 3.8m in with, 2.2m in eaves height, 2.9m in ridges height, and 6m in length. The width, eaves height, ridges height, and length of the arch shape small greenhouse covered with a 0.15mm PO film were 3m, 1.5m, 2.8m, and 6m, respectively.

Basaloid squamous cell carcinoma (BSCC) is a rare and aggressive variant of squamous cell carcinoma arising in various anatomical sites. This article describes a case history of BSCC of the floor of mouth with mandibular involvement, and further discusses the appropriate management of such case with reference to the literature review. A 52-year-old male patient was referred to our clinic from another university hospital. Segmental mandibulectomy with supraomohyoid neck dissection and mandible reconstruction with left fibular free flap under general anesthesia was performed, followed by radiotherapy. Histopathological examination on the tumor lesion revealed features of squamous cell carcinoma with comedo-type necrosis. A diagnosis of BSCC was given by the oral pathology specialist. Basaloid squamous cell carcinoma (BSCC) is a biphasic variant of SCC with both basaloid and squamous cell histology. A recent report showed that there is no significant difference in the prognosis. Due to the lack of accumulated research, close follow-up and continuous research are deemed necessary. Treatment that focuses on the stage of the tumor is appropriate. A periodic follow-up observation is also very important due to the occurrence of distant metastasis to the lungs.

Tissue engineering has been rapidly developed in oral and maxillofacial reconstruction. Biocompatible scaffold from chemically composites seeded with stem cells is essential and several growth factors for bone formation and angiogenesis are also required. To overcome limited activity of new bone formation with scaffolds, several biomechanical stimulation methods on cells have been made to grow cells in scaffold. Several bioreactors have been developed for real tissue growth in culture laboratory. In addition to biological stimulants like BMP, growth factors and exogenous drugs, biomechanical stimulation technique has also been known as an effective method in cell differentiation. We developed our own bioreactor with tensile mechanical strains. Then we tested with it for detection of suitable biomechanical effect on the cell differentiation and proliferation. And we also compared the results with the effect of low intensity pulsed ultrasound (LIPUS). Mechanical strain group showed more rapid reaction with cell differentiation and proliferation than non-mechanical strain group. Mechanical strain groups stimulated with 0.5∼0.7Hz for 6 hours and 8 hours showed more active cell differentiation than the group with 0.5∼0.7Hz for 2.5 hours tensile strain stimulation. Group of LIPUS also showed more rapid reaction in cell differentiation and proliferation. LIPUS with 3MHz showed more cell reaction than the LIPUS group with 1MHz. Our results showed the positive effect on differentiation and proliferation of cell with mechanical tensile strain, LIPUS both.

The vascular origin diseases occurring in the oral and maxillofacial region can be divided into hemangiomas and vascular malformation. They are rare and early lesions are not easy to diagnosis, so they may be misdiagnosed as clinicians, or the method and timing of treatment may be inappropriate. For 10 years, from August 2005 to August 2015, we analyzed the clinical records, diagnosis, treatment, and prognosis of 23 patients with hemangioma or vascular malformation confirmed by clinical, histopathological or radiological examination of maxillofacial lesions at department of oral and maxillofacial surgery, Pusan National University Hospital and Pusan National University Dental Hospital. Chief complants of patients were mainly swelling, mass, and spot-like lesion on various intraoral sites. Treatments were performed with excision, embolization, sclerotherapy, depending on each lesions condition. Arteriovenous malformation cases were more frequently recurred than other lesions. Prognosis of lesions were relatively good but close observation should be needed.

본 연구는 전국 임의의 산양삼 재배지를 선정하여 재배 지 내의 토양 특성 및 토양세균군집을 분석하고, 토양이 화학적 특성, 토양세균군집 및 산양삼 생육특성 간의 상관 관계를 구명하기 위하여 수행되었다. 토양세균군집 분석은 pyrosequencing analysis (Illumina platform)를 이용하였고, 토양세균군집과 생육특성 간의 상관관계는 Spearman’s rank correlation을 이용하여 분석하였다. 8개 산양삼 재배 지로부터 분리한 토양세균군집은 2개의 군집으로 군집화를 이루는 것을 확인하였다. 모든 토양샘플에서 Proteobacteria와 Alphaproteobacteria가 각각 35.4%, 24.4%로 가장 높은 상대적 빈도수를 보였다. 산양삼의 생육은 토양 pH가 낮고 Acidobacteria의 상대적 빈도수가 높은 토양에서 증가 하였으며, Acidobacteriia (class)와 Koribacteraceae (family)의 상대적 빈도수는 산양삼의 생육과 유의적인 정의 상관관계를 보이는 것으로 나타났다. 본 연구 결과는 토양세균군 집과 산양삼 생육 간의 상관관계를 구명하는 중요한 자료가 될 것으로 생각되고, 나아가 산양삼 재배 이전에 산양삼의 생육에 유용한 토양세균군집을 확인할 수 있다면 산양삼 재배적지를 선정하는데 도움을 줄 수 있을 것이다. 또한 토양이화학성과 더불어 임상 및 주변식생에 따른 토양 세균군집과 산양삼 생육특성에 대한 상관관계 연구를 추 가로 수행한다면 보다 명확한 정보를 대한 제공할 수 있을 것으로 사료된다.

We used three gas sensors to monitor hydrogen sulfide, ammonia, and volatile organic compounds (VOCs), which were frequently emitted from environmental facilities, such as municipal wastewater treatment, livestock manure treatment, and food waste composting facilities. Two electrochemical (EC) sensors for detecting hydrogen sulfide and ammonia, and a photoionization detector (PID) sensor for detecting VOCs were characterized in this study. The performance of their linearity by concentration levels, lower detection limit (LDL), repeatability, reproducibility, precision, and response time were tested under the laboratory condition. The linearity according to concentration levels were favorable for all three sensors with high correlation coefficients (R2 > 0.98). The ammonia sensor showed the highest LDL (18.6 ppb) and the hydrogen sulfide and VOC sensors showed 22.3 ppb and 26.7 ppb of LDL, respectively. The reproducibility and precision were favorable for all three sensors, indicating a lower relative standard deviation (RSD) than 0.9% in the reproducibility test and 7.2% in the precision test. The response times to reach target concentration were varied from 1 to 12 minutes. The ammonia sensor needed 12 minutes of response time at 1 ppm target the NH3 concentration and the hydrogen sulfide and VOC sensors needed less than 2 minutes of response time.

고위험해충의 국내 유입 및 발생 여부를 조사하기 위한 예찰협력 네트워크 구성을 위해 7개 대학(경상대학교, 군산대학교, 서울대학교, 순천대학교, 안동대학교, 제주대학교, 충북대학교)이 참여하여 고위험해충 9종(Aceria diospyri, 오리엔탈과실파리(Bactrocera dorsalis), 일본과실 파리(Bactrocera minax), Bactrocera tsuneonis, 코드린나방(Cydia pomonella), 포도애기잎말이나방(Lobesia botrana), Proeulia sp., 붉은불개미 (Solenopsis invicta), Stephanitis takeyai)에 대한 예찰조사를 2018년 6월부터 10월까지 실시하였다. 전국을 7개 권역으로 구분한 뒤, 105개 지역 내 315개 지점에서 총 7,560개의 트랩운용/달관조사를 실시하였으며, 조사결과 A. diospyri, 오리엔탈과실파리, 일본과실파리, B. tsuneonis, C. pomonella, 포도애기잎말이나방, Proeulia sp., 붉은불개미, S. takeyai는 발견되지 않았다. 본 조사 연구를 통하여 고위험해충을 조기 탐지할 수 있는 전국단위의 감시체계를 구축하였으며, 국경 이후 외래침입해충들의 예찰조사를 위한 거점 지역을 확보하였다.

This study was designed as the precedent research to develop improve the HMR type flavored cooked rice, by analyzing the consumer perception and purchase pattern. This will in turn provide base data to that will help in the development of products that meet consumer’s convenience and quality demands. An online survey of 247 people (63.5%) in the age of 20 to 60 was conducted on January 2019. For the primary reason of buying those products, the survey respondents chose a convenience of 71.7%. They are likely to buy 1~2 times a month (40.5%) in hypermarkets (63.6%). The channel choice showed meaningful difference between segments (p<0.01). 89.1% of respondents replied that they are willing to purchase frozen flavored cooked rice in the future. The Key Purchasing factor appeared to be the flavor (5.80), while nutrition (4.89) was chosen as the least important factor. For flavor preference, sea food received the highest score (5.05), while radish kimchi flavor received the lowest (4.02) with a meaningful difference by gender and age (p<0.01). Consumers who sought nutrition, convenience and adventure in HMR products had higher willingness to re-purchase. Developing healthy, flavorful, sensory, and enjoyable HMR products will be critical in the fulfillment of diverse consumer needs.

본 연구에서는 건조농산물의 잔류농약 안전성 조사를 위해 경기도에서 유통되는 건조농산물 110건을 수거하여 다종농약다성분 분석법으로 잔류농약 263종을 검사하였다. 조사 결과 총 10건의 시료에서 10종의 잔류농약이 검출되었으며, 건피마자에서 chlorpyrifos, 건취나물에서 chlorpyrifos, hexaconazole, pyridalyl, 무시래기에서 diniconazole, isoprothiolane, lufenuron, 건곤드레에서 hexaconazole, 건고춧 잎에서 bifenthrin, chlorothalonil, boscalid, pyraclostrobin이 각각 검출되었다. 검출률은 9.1%였으며, 이 중 건피마자 1 건에서 chlorpyrifos가 잔류허용기준을 초과하였고 부적합률은 0.9%로 나타났다. 유효성 검증 실험 결과 검출한계(LOD)는 0.002~0.027 mg/kg, 정량한계(LOQ)는 0.006~0.083 mg/kg 으로 나타났으며, 결정계수(R2)는 0.9964~1.0000으로 나타났다. 회수율은 전반적으로 74.8~118.9%로 나타났다. 2019 년부터 PLS가 시행됨에 따라 농산물에 대한 안전성 관리가 강화되었지만 건조농산물의 경우 잔류허용기준이 미비 하기 때문에 본 연구는 향후 기준 설정을 위한 기초자료로 제공하고자 한다.

Squamous odontogenic tumor (SOT) is a relatively rare, benign, small (rarely > 1.5 cm in diameter), but locally infiltrative and occasionally aggressive odontogenic epithelial lesion that appears to have harmatomous and neoplastic characteristics [1]. SOT is often asymptomatic, although it can present with mildly painful gingival swelling or tooth mobility. Radiographically, SOT is usually visualized as an irregularly or triangularly shaped unilocular radiolucency associated with the lateral roots of teeth. We report a case of the squamous odontogenic tumor occurring in a 60-year-old female in the right posterior maxilla with unusual radiographic and clinical appearances.

The multi-layer insulating curtains used in the experiment was produced in six combinations using non-woven fabric containing aerogel and compared and analyzed by measuring heat flux and heat perfusion rates due to weight, thickness and temperature changes. Using silica aerogel, which have recently been noted as new material insulation, this study tries to produce a new combination of multi-layer insulating curtains that can complement the shortcomings of the multi-layer insulating curtains currently in use and maintain and improve its warmth, and analyze the thermal properties. The heat flux means the amount of heat passing per unit time per unit area, and the higher the value, the more heat passing through the multi-layer insulating curtain, and it can be judged that the heat retention is low. The weight and thickness of multi-layer insulation curtains were found to be highly correlated with thermal insulation. In particular, insulation curtains combined with aerogel meltblown non-woven fabric had relatively higher thermal insulation than insulation curtains with the same number of insulation materials. However, the aerogel meltblown non-woven fabric is weak in light resistance and durability, and there is a problem that the production process and aerogel are scattering. In order to solve this problems, the combination of expanded aerogel non-woven fabric and hollow fiber non-woven fabric, which are relatively simple manufacturing processes and excellent warmth, are suitable for use in real farms.